Site Directed Mutagenesis / RFLP Calculator

The yeast mutation calculator allows you to change a codon to introduce a single amino acid change and then see all the sequence permutations in the 15 nucleotides surrounding that change. A list of restriction sites is generated for each sequence permutation to help define useful restriction polymorphisms for the mutated sequence. In addition, the product of the codon frequencies for the sequence are listed for comparison to the wild-type sequence.

The sequence should be 15 nt in length and be in frame so that the middle codon is the mutated codon.

For example,CTGGGCACTTCCAAAtranslates to:
where T is the amino acid to be mutated.

15nt sequence: