mat a Synced to SGD on Thu Dec 24 14:41:25 2015 Plate # Row Column ORF Gene Alias Description GO Biological Process GO Molecular Function GO Cellular Component record no. strain batch 1 A 2 YAL068C PAU8 seripauperin PAU8 Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions meiotic cell cycle, sporulation, transcription from RNA polymerase II promoter molecular_function cellular_component 338 BY4741 01_1 1 A 3 YAL067C SEO1 putative permease SEO1 Putative permease; member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide other other membrane 339 BY4741 01_1 1 A 4 YAL066W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 340 BY4741 01_1 1 A 5 YAL065C - - Putative protein of unknown function; shows sequence similarity to FLO1 and other flocculins biological_process molecular_function cellular_component 341 BY4741 01_1 1 A 6 YAL062W GDH3 glutamate dehydrogenase (NADP(+)) GDH3|FUN51 NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication cellular amino acid metabolic process oxidoreductase activity nucleus, mitochondrion, cytoplasm 345 BY4741 01_1 1 A 7 YAL061W BDH2 putative dehydrogenase BDH2 Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 biological_process oxidoreductase activity nucleus, cytoplasm 346 BY4741 01_1 1 A 8 YAL060W BDH1 (R,R)-butanediol dehydrogenase|BDH NAD-dependent (R,R)-butanediol dehydrogenase; catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source other oxidoreductase activity cytoplasm 347 BY4741 01_1 1 A 9 YAL059W ECM1 - Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm ribosomal subunit export from nucleus, nuclear transport molecular_function nucleus, nucleolus 348 BY4741 01_1 1 A 10 YAL058W CNE1 calnexin|FUN48 Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast response to chemical, proteolysis involved in cellular protein catabolic process, protein folding unfolded protein binding endomembrane system, membrane, endoplasmic reticulum, cytoplasm 349 BY4741 01_1 1 A 11 YAL056W GPB2 KRH1 Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication meiotic cell cycle, response to chemical, invasive growth in response to glucose limitation, sporulation, pseudohyphal growth, transcription from RNA polymerase II promoter, signaling enzyme regulator activity, signal transducer activity mitochondrion, membrane, cytoplasm, plasma membrane 351 BY4741 01_1 1 A 12 YAL055W PEX22 ubiquitin-protein transferase activating protein PEX22|YAF5 Putative peroxisomal membrane protein; required for import of peroxisomal proteins; functionally complements a Pichia pastoris pex22 mutation protein targeting, transmembrane transport, peroxisome organization, regulation of protein modification process, protein modification by small protein conjugation or removal, signaling enzyme regulator activity peroxisome, membrane, cytoplasm 352 BY4741 01_1 1 B 1 YAL053W FLC2 flavin adenine dinucleotide transporter FLC2|HUF2 Putative calcium channel involved in calcium release under hypotonic stress; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication transmembrane transport, ion transport, nucleobase-containing compound transport, protein folding, cell wall organization or biogenesis, response to osmotic stress transmembrane transporter activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 354 BY4741 01_1 1 B 2 YAL051W OAF1 oleate-activated transcription factor OAF1|YAF1 Oleate-activated transcription factor; subunit of a heterodimeric complex with Pip2p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; regulates chromatin silencing at telomeres; involved in diauxic shift; OAF1 has a paralog, PIP2, that arose from the whole genome duplication response to chemical, chromatin organization, regulation of organelle organization, monocarboxylic acid metabolic process, lipid metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 355 BY4741 01_1 1 B 3 YAL049C AIM2 protein AIM2 Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor biological_process molecular_function cytoplasm 356 BY4741 01_1 1 B 4 YAL048C GEM1 ERMES complex Ca(2+)-binding regulatory GTPase GEM1|GON1 Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases mitochondrion organization, regulation of organelle organization, organelle inheritance GTPase activity, hydrolase activity, ion binding endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 357 BY4741 01_1 1 B 5 YAL046C AIM1 - Protein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular_component 359 BY4741 01_1 1 B 6 YAL045C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A biological_process molecular_function cellular_component 360 BY4741 01_1 1 B 7 YAL044C GCV3 glycine decarboxylase subunit H H subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 361 BY4741 01_1 1 B 8 YAL042W ERV46 FUN9 Protein localized to COPII-coated vesicles; forms a complex with Erv41p; involved in the membrane fusion stage of transport Golgi vesicle transport molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, endoplasmic reticulum, cytoplasm, Golgi apparatus 363 BY4741 01_1 1 B 9 YAL043C-a membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, endoplasmic reticulum, cytoplasm, Golgi apparatus membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, endoplasmic reticulum, cytoplasm, Golgi apparatus membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, endoplasmic reticulum, cytoplasm, Golgi apparatus - - - 364 BY4741 01_1 1 B 10 YAL040C CLN3 cyclin CLN3|WHI1|FUN10|DAF1 G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote G1 to S phase transition; plays a role in regulating transcription of other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis; acetyl-CoA induces CLN3 transcription in response to nutrient repletion to promote cell-cycle entry; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1 vacuole organization, mitotic cell cycle, protein phosphorylation, organelle fusion, membrane fusion, regulation of protein modification process, transcription from RNA polymerase II promoter, regulation of cell cycle enzyme regulator activity nucleus 366 BY4741 01_1 1 B 11 YAL039C CYC3 holocytochrome c synthase CYC3|CCHL Cytochrome c heme lyase (holocytochrome c synthase); attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in mitochondrial intermembrane space; human homolog HCCS implicated in microphthalmia with linear skin defects (MLS), and can complement yeast null mutant protein complex biogenesis lyase activity mitochondrial envelope, mitochondrion, cytoplasm 367 BY4741 01_1 1 B 12 YAL037W - - Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication biological_process molecular_function cellular_component 369 BY4741 01_1 1 C 1 YAL036C RBG1 GTP-binding protein RBG1|FUN11 Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid cytoplasmic translation ion binding ribosome, cytoplasm 370 BY4741 01_1 1 C 2 YAL035W FUN12 translation initiation factor eIF5B|eIF5B|yIF2 Translation initiation factor eIF5B; GTPase that promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2 cytoplasmic translation, translational initiation, regulation of translation, rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly RNA binding, GTPase activity, hydrolase activity, ion binding, translation factor activity, RNA binding ribosome, mitochondrion, cytoplasm 371 BY4741 01_1 1 C 3 YAL034C FUN19 - Non-essential protein of unknown function; expression induced in response to heat stress; FUN19 has a paralog, YOR338W, that arose from the whole genome duplication biological_process molecular_function cellular_component 374 BY4741 01_1 1 C 4 YAL031C GIP4 protein phosphatase regulator GIP4|FUN21 Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; protein overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate regulation of protein modification process, protein dephosphorylation, chromosome segregation enzyme binding, enzyme regulator activity cytoplasm 377 BY4741 01_1 1 C 5 YAL030W SNC1 SNAP receptor SNC1 Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication organelle fusion, vesicle organization, membrane fusion, Golgi vesicle transport, exocytosis, endocytosis other endomembrane system, cellular bud, cytoplasmic membrane-bounded vesicle, membrane, site of polarized growth, Golgi apparatus, cytoplasm, plasma membrane 378 BY4741 01_1 1 C 6 YAL029C MYO4 myosin 4|SHE1|FUN22 Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication organelle inheritance cytoskeletal protein binding, hydrolase activity cellular bud, site of polarized growth, cytoskeleton, mitochondrion, cytoplasm 379 BY4741 01_1 1 C 7 YAL028W FRT2 HPH2 Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication response to osmotic stress molecular_function endomembrane system, endoplasmic reticulum, cytoplasm 380 BY4741 01_1 1 C 8 YAL027W SAW1 DNA-binding protein SAW1 5'- and 3'-flap DNA binding protein; recruits Rad1p-Rad10p to single-strand annealing intermediates with 3' non-homologous tails for removal during double-strand break repair; complexes with Rad1p-Rad10p and stimulates its endonuclease activity; green fluorescent protein (GFP)-fusion protein localizes to the nucleus DNA repair, regulation of DNA metabolic process, cellular response to DNA damage stimulus DNA binding nucleus 381 BY4741 01_1 1 C 9 YAL026C DRS2 aminophospholipid-translocating P4-type ATPase DRS2|SWA3|FUN38 Trans-golgi network aminophospholipid translocase (flippase); maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation, endocytosis, protein trafficking between the Golgi and endosomal system and the cellular response to mating pheromone; autoinhibited by its C-terminal tail; localizes to the trans-Golgi network; mutations in human homolog ATP8B1 result in liver disease response to chemical, ribosome assembly, Golgi vesicle transport, lipid transport, conjugation, ion transport, organelle assembly, ribosomal small subunit biogenesis, endosomal transport, endocytosis hydrolase activity, ATPase activity Golgi apparatus, endomembrane system, membrane, cytoplasm 382 BY4741 01_1 1 C 10 YAL023C PMT2 dolichyl-phosphate-mannose-protein mannosyltransferase PMT2|FUN25 Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication carbohydrate metabolic process, response to chemical, proteolysis involved in cellular protein catabolic process, protein glycosylation, signaling transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 385 BY4741 01_1 1 C 11 YAL022C FUN26 nucleoside transmembrane transporter FUN26 High affinity, broad selectivity, nucleoside/nucleobase transporter; vacuolar membrane localized transporter which may regulate the balance of nicotinamide riboside (NmR) levels between the cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis; equilibrative nucleoside transporter (ENT) family member transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, vacuole, cytoplasm 386 BY4741 01_1 1 C 12 YAL021C CCR4 CCR4-NOT core exoribonuclease subunit CCR4|NUT21|FUN27 Component of the CCR4-NOT transcriptional complex; CCR4-NOT is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening mitotic cell cycle, DNA replication, RNA catabolic process, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, regulation of cell cycle, DNA-templated transcription, elongation hydrolase activity, nuclease activity nucleus, cytoplasm 387 BY4741 01_1 1 D 1 YAL020C ATS1 KTI13|FUN28 Protein required for modification of wobble nucleosides in tRNA; acts with Elongator complex, Kti11p, and Kti12p; has a potential role in regulatory interactions between microtubules and the cell cycle; forms a stable heterodimer with Kti11p tRNA processing, cell budding, cytoskeleton organization, RNA modification molecular_function cytoplasm 388 BY4741 01_1 1 D 2 YAL019W FUN30 DNA-dependent ATPase FUN30 Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate DNA repair, chromatin organization, cellular response to DNA damage stimulus, transcription from RNA polymerase II promoter chromatin binding, hydrolase activity, DNA binding, ATPase activity nucleus, chromosome, mitochondrion, cytoplasm 389 BY4741 01_1 1 D 3 YAL018C LDS1 - Protein Involved in spore wall assembly; localizes to lipid droplets found on or outside of the prospore membrane; shares similarity with Lds2p and Rrt8p, and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function cell wall, membrane 390 BY4741 01_1 1 D 4 YAL017W PSK1 serine/threonine protein kinase PSK1|FUN31 PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication carbohydrate metabolic process, protein phosphorylation, generation of precursor metabolites and energy kinase activity, transferase activity cytoplasm 391 BY4741 01_1 1 D 5 YAL015C NTG1 bifunctional N-glycosylase/AP lyase NTG1|ogg2|SCR1|FUN33 DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; required for maintaining mitochondrial genome integrity; NTG1 has a paralog, NTG2, that arose from the whole genome duplication DNA repair, response to chemical, DNA replication, mitochondrion organization, regulation of organelle organization, cellular response to DNA damage stimulus, response to oxidative stress, regulation of DNA metabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds, lyase activity, nuclease activity nucleus, mitochondrion, cytoplasm 393 BY4741 01_1 1 D 6 YAL014C SYN8 syntaxin|SLT2|UIP2 Endosomal SNARE related to mammalian syntaxin 8 other other endomembrane system, cytoplasm 394 BY4741 01_1 1 D 7 YAL013W DEP1 Rpd3L histone deacetylase complex subunit DEP1|FUN54 Component of the Rpd3L histone deacetylase complex; required for diauxic shift-induced histone H2B deposition onto rDNA genes; transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation response to heat, carbohydrate metabolic process, chromatin organization, DNA replication, regulation of organelle organization, histone modification, cellular amino acid metabolic process, lipid metabolic process, regulation of DNA metabolic process, transcription from RNA polymerase II promoter molecular_function nucleus, chromosome 395 BY4741 01_1 1 D 8 YAL011W SWC3 SWC1 Protein of unknown function; component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae chromatin organization molecular_function nucleus, chromosome, mitochondrion, cytoplasm 397 BY4741 01_1 1 D 9 YAL010C MDM10 FUN37 Subunit of both the ERMES and the SAM complex; component of ERMES complex which acts as a molecular tether between the mitochondria and the ER, necessary for efficient phospholipid exchange between organelles and for mitophagy; SAM/TOB complex component that functions in the assembly of outer membrane beta-barrel proteins; involved in mitochondrial inheritance and morphology; ERMES complex is often co-localized with peroxisomes and concentrated areas of pyruvate dehydrogenase protein targeting, mitochondrion organization, transmembrane transport, ion transport, protein complex biogenesis, lipid transport molecular_function endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 398 BY4741 01_1 1 D 10 YAL009W SPO7 Nem1-Spo7 phosphatase regulatory subunit SPO7 Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation protein dephosphorylation, lipid metabolic process, regulation of protein modification process, nucleus organization phosphatase activity, hydrolase activity endomembrane system, membrane 399 BY4741 01_1 1 D 11 YAL008W FUN14 - Mitochondrial protein of unknown function biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 400 BY4741 01_1 1 D 12 YAL007C ERP2 - Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP2 has a paralog, ERP4, that arose from the whole genome duplication Golgi vesicle transport molecular_function cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm 401 BY4741 01_1 1 E 1 YAL004W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C biological_process molecular_function cellular_component 402 BY4741 01_1 1 E 2 YAL005C SSA1 Hsp70 family ATPase SSA1|YG100 ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils cytoplasmic translation, transmembrane transport, protein targeting, mitochondrion organization, protein folding, protein modification by small protein conjugation or removal, nuclear transport RNA binding, hydrolase activity, unfolded protein binding, ATPase activity nucleus, vacuole, cell wall, plasma membrane, membrane, cytoplasm 403 BY4741 01_1 1 E 3 YAL002W VPS8 CORVET complex membrane-binding subunit VPS8|VPL8|VPT8|FUN15 Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif protein targeting, endosomal transport enzyme binding membrane, endomembrane system, cytoplasm 405 BY4741 01_1 1 E 4 YAR002W NUP60 FG-nucleoporin NUP60 FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; relocalizes to the cytosol in response to hypoxia; both NUP1 and NUP60 are homologous to human NUP153 response to heat, peptidyl-amino acid modification, chromatin organization, DNA repair, protein targeting, cellular response to DNA damage stimulus, nucleobase-containing compound transport, regulation of protein modification process, protein modification by small protein conjugation or removal, nuclear transport ion binding, lipid binding, structural molecule activity nucleus, endomembrane system, membrane, cytoplasm 407 BY4741 01_1 1 E 5 YAR003W SWD1 COMPASS subunit protein SWD1|SAF49|CPS50|FUN16 Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 peptidyl-amino acid modification, telomere organization, protein alkylation, chromatin organization, histone modification transferase activity, methyltransferase activity nucleus 408 BY4741 01_1 1 E 6 YAR014C BUD14 protein phosphatase regulator BUD14 Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; forms a complex with Kel1p and Kel2p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress cell morphogenesis, cytoskeleton organization, regulation of organelle organization, cytokinesis, regulation of transport, protein complex biogenesis, regulation of cell cycle enzyme regulator activity nucleus, cellular bud, site of polarized growth, cytoplasm 413 BY4741 01_1 1 E 7 YAR015W ADE1 phosphoribosylaminoimidazolesuccinocarboxamide synthase N-succinyl-5-aminoimidazole-4-carboxamide ribotide synthetase; required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress nucleobase-containing small molecule metabolic process ligase activity nucleus, cytoplasm 414 BY4741 01_1 1 E 8 YAR018C KIN3 serine/threonine protein kinase KIN3|NPK1|FUN52 Nonessential serine/threonine protein kinase; possible role in DNA damage response; influences tolerance to high levels of ethanol protein phosphorylation, chromosome segregation kinase activity, transferase activity cellular_component 415 BY4741 01_1 1 E 9 YAR020C PAU7 seripauperin PAU7 Member of the seripauperin multigene family; active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme biological_process molecular_function cellular_component 417 BY4741 01_1 1 E 10 YAR023C - DUP240 family protein Putative integral membrane protein; member of DUP240 gene family biological_process molecular_function cellular_component 418 BY4741 01_1 1 E 11 YAR027W UIP3 DUP240 family protein UIP3 Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family biological_process molecular_function nucleus, endomembrane system 419 BY4741 01_1 1 E 12 YAR028W - DUP240 family protein Putative integral membrane protein; member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function membrane, vacuole, cytoplasm 420 BY4741 01_1 1 F 1 YAR029W - DUP240 family protein Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function cytoplasm 421 BY4741 01_1 1 F 2 YAR031W PRM9 pheromone-regulated DUP240 family protein PRM9 Pheromone-regulated protein; contains 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family; PRM9 has a paralog, PRM8, that arose from a segmental duplication biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, plasma membrane, cytoplasm 422 BY4741 01_1 1 F 3 YAR030C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 biological_process molecular_function cellular_component 423 BY4741 01_1 1 F 4 YAR035W YAT1 carnitine O-acetyltransferase YAT1 Outer mitochondrial carnitine acetyltransferase; minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated monocarboxylic acid metabolic process transferase activity mitochondrion, cytoplasm 425 BY4741 01_1 1 F 5 YAR037W mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - 426 BY4741 01_1 1 F 6 YAR040C - - - - - - 427 BY4741 01_1 1 F 7 YAR042W SWH1 oxysterol-binding protein related protein SWH1|OSH1|YAR044W Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats and FFAT motif; interacts with ER anchor Scs2p at the nucleus-vacuole junction; regulated by sterol binding; SWH1 has a paralog, OSH2, that arose from the whole genome duplication lipid transport, membrane invagination, exocytosis, endocytosis lipid binding, ion binding nucleus, endomembrane system, endoplasmic reticulum, Golgi apparatus, cytoplasm 428 BY4741 01_1 1 F 8 YAR043C nucleus, endomembrane system, endoplasmic reticulum, Golgi apparatus, cytoplasm nucleus, endomembrane system, endoplasmic reticulum, Golgi apparatus, cytoplasm nucleus, endomembrane system, endoplasmic reticulum, Golgi apparatus, cytoplasm - - - 429 BY4741 01_1 1 F 9 YAR044W - - - - - - 430 BY4741 01_1 1 F 10 YAR047C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 431 BY4741 01_1 1 F 11 YLL001W DNM1 dynamin-related GTPase DNM1 Dynamin-related GTPase involved in mitochondrial organization; required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance; human homolog Drp1 plays an important role in mediating maintenance of mitochondrial function, autophagy, and mitochondrial autophagy in the heart and cardiomyocytes organelle fission, mitochondrion organization, peroxisome organization, protein complex biogenesis, organelle inheritance GTPase activity, hydrolase activity peroxisome, mitochondrion, membrane, mitochondrial envelope, cytoplasm 1489 BY4741 12_1 1 F 12 YLL002W RTT109 H3 histone acetyltransferase RTT109|KAT11|REM50|KIM2 Histone acetyltransferase; critical for cell survival in the presence of DNA damage during S phase; prevents hyper-amplification of rDNA; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p peptidyl-amino acid modification, DNA repair, protein acylation, chromatin organization, cellular response to DNA damage stimulus, transposition, histone modification, transcription from RNA polymerase II promoter transferase activity nucleus 1490 BY4741 12_1 1 G 1 YLL005C SPO75 - Meiosis-specific protein of unknown function; required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function membrane 1493 BY4741 12_1 1 G 2 YLL006W MMM1 ERMES complex subunit MMM1|YME6 ER integral membrane protein, ERMES complex subunit; ERMES links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase protein targeting, mitochondrion organization, transmembrane transport, ion transport, protein complex biogenesis, lipid transport molecular_function endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 1494 BY4741 12_1 1 G 3 YLL009C COX17 copper metallochaperone COX17 Copper metallochaperone that transfers copper to Sco1p and Cox11p; eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs; interacts with the MICOS complex, and interaction is promoted by copper ions; human homolog COX17 partially complements yeast null mutant mitochondrion organization, ion transport, protein complex biogenesis ion binding mitochondrial envelope, mitochondrion, cytoplasm 1497 BY4741 12_1 1 G 4 YLL010C PSR1 - Plasma membrane associated protein phosphatase; involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p; PSR1 has a paralog, PSR2, that arose from the whole genome duplication response to osmotic stress phosphatase activity, hydrolase activity membrane, plasma membrane 1498 BY4741 12_1 1 G 5 YLL012W YEH1 sterol esterase Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes; YEH1 has a paralog, YEH2, that arose from the whole genome duplication lipid metabolic process hydrolase activity membrane 1500 BY4741 12_1 1 G 6 YLL013C PUF3 mRNA-binding protein PUF3 Protein of the mitochondrial outer surface; links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins mitochondrion organization, generation of precursor metabolites and energy, cellular respiration, RNA catabolic process RNA binding, mRNA binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 1501 BY4741 12_1 1 G 7 YLL014W EMC6 - Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 protein folding molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1502 BY4741 12_1 1 G 8 YLL015W BPT1 ATP-binding cassette bilirubin transporter BPT1 ABC type transmembrane transporter of MRP/CFTR family; found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p vacuole organization, ion transport, organelle fusion, membrane fusion transmembrane transporter activity vacuole, membrane, cytoplasm 1503 BY4741 12_1 1 G 9 YLL016W vacuole, membrane, cytoplasm vacuole, membrane, cytoplasm vacuole, membrane, cytoplasm - - - 1504 BY4741 12_1 1 G 10 YLL017W - - - - - - 1505 BY4741 12_1 1 G 11 YLL019C KNS1 serine/threonine protein kinase KNS1|L124 Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation peptidyl-amino acid modification, protein phosphorylation, transcription from RNA polymerase III promoter kinase activity, transferase activity nucleus, cytoplasm 1507 BY4741 12_1 1 G 12 YLL020C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 biological_process molecular_function cellular_component 1508 BY4741 12_1 1 H 1 YLL021W SPA2 PPF1|PEA1|FUS6 Component of the polarisome; functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate; coding sequence contains length polymorphisms in different strains; SPA2 has a paralog, SPH1, that arose from the whole genome duplication mitotic cell cycle, response to chemical, cell morphogenesis, cell budding, cytoskeleton organization, regulation of organelle organization, cytokinesis, conjugation, pseudohyphal growth enzyme binding, structural molecule activity cell cortex, cellular bud, site of polarized growth, cytoplasm 1509 BY4741 12_1 1 H 3 YLL023C POM33 nucleoporin POM33 Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress protein complex biogenesis, nucleus organization structural molecule activity nucleus, endomembrane system, ribosome, membrane, endoplasmic reticulum, cytoplasm 1511 BY4741 12_1 1 H 4 YLL024C SSA2 Hsp70 family chaperone SSA2|YG102 ATP-binding protein; involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical to paralog Ssa1p, but has distinct functional specificity in propagation of yeast [URE3] prions and vacuolar-mediated degradation of gluconeogenesis enzymes; binds tRNA and has a role in tRNA nuclear import during starvation protein targeting, transmembrane transport, nucleobase-containing compound transport, protein folding, nuclear transport RNA binding, hydrolase activity, unfolded protein binding, ion binding, ATPase activity vacuole, mitochondrion, cell wall, membrane, cytoplasm, plasma membrane 1512 BY4741 12_1 1 H 5 YLL025W PAU17 seripauperin PAU17 Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene biological_process molecular_function cellular_component 1513 BY4741 12_1 1 H 6 YLL026W HSP104 chaperone ATPase HSP104 Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; potentiated Hsp104p variants decrease TDP-43 proteotoxicity by eliminating its cytoplasmic aggregation response to heat, carbohydrate metabolic process, response to chemical, response to oxidative stress, protein folding, oligosaccharide metabolic process hydrolase activity, ion binding, unfolded protein binding, ATPase activity nucleus, cytoplasm 1514 BY4741 12_1 1 H 7 YLL028W TPO1 - Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; during oxidative stress exports spermine, spermidine from the cell, which controls timing of expression of stress-responsive genes; phosphorylation enhances activity and sorting to the plasma membrane transmembrane transport, ion transport transmembrane transporter activity vacuole, cellular bud, membrane, cytoplasm, plasma membrane 1516 BY4741 12_1 1 H 8 YLL029W FRA1 RUP2 Protein involved in negative regulation of iron regulon transcription; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation response to chemical, transcription from RNA polymerase II promoter molecular_function cytoplasm 1517 BY4741 12_1 1 H 9 YLL032C - - Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene biological_process RNA binding, mRNA binding ribosome, cytoplasm 1520 BY4741 12_1 1 H 10 YLL033W IRC19 RRG4|GON2 Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci meiotic cell cycle, sporulation, DNA recombination molecular_function cellular_component 1521 BY4741 12_1 1 H 11 YLL038C ENT4 - Protein of unknown function; contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome cytoskeleton organization, endocytosis other cell cortex, cytoskeleton, cytoplasm 1526 BY4741 12_1 1 H 12 YLL039C UBI4 ubiquitin|UB14|SCD2 Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress meiotic cell cycle, sporulation, protein modification by small protein conjugation or removal other cytoplasm 1527 BY4741 12_1 2 A 1 YLL040C VPS13 membrane morphogenesis protein VPS13|VPT2|SOI1 Protein involved in prospore membrane morphogenesis; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation during sporulatoin, and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively meiotic cell cycle, protein targeting, sporulation, endosomal transport molecular_function endomembrane system, mitochondrion, membrane, cytoplasm 1528 BY4741 12_1 2 A 3 YLL041C SDH2 succinate dehydrogenase iron-sulfur protein subunit SDH2|SDHB|ACN17 Iron-sulfur protein subunit of succinate dehydrogenase; the complex couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; other members are Sdh1p, Sdh3p, and Sdh4p generation of precursor metabolites and energy, cellular respiration oxidoreductase activity vacuole, mitochondrion, membrane, mitochondrial envelope, cytoplasm 1529 BY4741 12_1 2 A 4 YLL042C ATG10 E2-like conjugating enzyme|APG10 Conserved E2-like conjugating enzyme; mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy protein targeting, membrane invagination, mitochondrion organization transferase activity cellular_component 1530 BY4741 12_1 2 A 5 YLL043W FPS1 - Aquaglyceroporin, plasma membrane channel; involved in efflux of glycerol and xylitol, and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; role in mediating passive diffusion of glycerol is key factor in maintenance of redox balance; phosphorylated by Hog1p MAPK under acetate stress; deletion improves xylose fermentation; regulated by Rgc1p and Ask10p, which themselves are regulated by Hog1p phosphorylation under osmotic stress response to chemical, ion transport, conjugation, carbohydrate transport transmembrane transporter activity vacuole, membrane, cytoplasm, plasma membrane 1531 BY4741 12_1 2 A 6 YLL045C RPL8B ribosomal 60S subunit protein L8B|L8e|rp6|YL5|L8B|L4B|SCL41|KRB1 Ribosomal 60S subunit protein L8B; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal large subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 1533 BY4741 12_1 2 A 7 YLL046C RNP1 - Ribonucleoprotein that contains two RNA recognition motifs (RRM); RNP1 has a paralog, SBP1, that arose from the whole genome duplication other RNA binding cytoplasm 1534 BY4741 12_1 2 A 8 YLL047W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 biological_process molecular_function cellular_component 1535 BY4741 12_1 2 A 9 YLL051C FRE6 putative ferric-chelate reductase Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels cellular ion homeostasis, ion transport oxidoreductase activity membrane, vacuole, cytoplasm 1539 BY4741 12_1 2 A 10 YLL052C AQY2 - Water channel that mediates water transport across cell membranes; only expressed in proliferating cells; controlled by osmotic signals; may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains transmembrane transport transmembrane transporter activity membrane, endomembrane system, endoplasmic reticulum, plasma membrane, cytoplasm 1540 BY4741 12_1 2 A 11 YLL053C - - Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin biological_process molecular_function membrane 1541 BY4741 12_1 2 A 12 YLL054C - - Putative protein of unknown function with similarity to Pip2p; an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene biological_process nucleic acid binding transcription factor activity, DNA binding cellular_component 1542 BY4741 12_1 2 B 1 YLL055W YCT1 - High-affinity cysteine-specific transporter; has similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene ion transport, amino acid transport transmembrane transporter activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1543 BY4741 12_1 2 B 2 YLL056C - - Putative protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin biological_process molecular_function cellular_component 1544 BY4741 12_1 2 B 3 YLL057C JLP1 - Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase; involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation other oxidoreductase activity cellular_component 1545 BY4741 12_1 2 B 4 YLL058W - cystathionine gamma-synthase Putative protein of unknown function with similarity to Str2p; Str2p is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene other transferase activity cellular_component 1546 BY4741 12_1 2 B 5 YLL060C GTT2 glutathione transferase GTT2 Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress other transferase activity mitochondrion, cytoplasm 1548 BY4741 12_1 2 B 6 YLL061W MMP1 S-methylmethionine permease MMP1 High-affinity S-methylmethionine permease; required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p other transmembrane transporter activity membrane, plasma membrane 1549 BY4741 12_1 2 B 7 YLL062C MHT1 S-adenosylmethionine-homocysteine S-methyltransferase MHT1 S-methylmethionine-homocysteine methyltransferase; functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio cellular amino acid metabolic process transferase activity, methyltransferase activity cytoplasm 1550 BY4741 12_1 2 B 8 YLL063C AYT1 - Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis other transferase activity cellular_component 1551 BY4741 12_1 2 B 9 YLR001C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated biological_process molecular_function membrane, vacuole, mitochondrion, cytoplasm 1556 BY4741 12_1 2 B 10 YLR003C CMS1 - Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress biological_process molecular_function nucleus, cytoplasm 1558 BY4741 12_1 2 B 11 YLR004C THI73 - Putative plasma membrane permease; proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs other other membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1559 BY4741 12_1 2 B 12 YLR011W LOT6 flavin-dependent quinone reductase FMN-dependent NAD(P)H:quinone reductase; role in apoptosis-like cell death; may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions response to chemical, protein targeting, regulation of transport, response to oxidative stress, nuclear transport oxidoreductase activity, transcription factor binding nucleus, cytoplasm 1566 BY4741 12_1 2 C 1 YLR012C - - Putative protein of unknown function; YLR012C is not an essential gene biological_process molecular_function cellular_component 1567 BY4741 12_1 2 C 2 YLR013W GAT3 - Protein containing GATA family zinc finger motifs; involved in spore wall assembly; sequence similarity to GAT4, and the double mutant gat3 gat4 exhibits reduced dityrosine fluorescence relative to the single mutants other nucleic acid binding transcription factor activity, DNA binding nucleus 1568 BY4741 12_1 2 C 3 YLR014C PPR1 - Zinc finger transcription factor; contains a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p nucleobase-containing small molecule metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding, ion binding nucleus 1569 BY4741 12_1 2 C 4 YLR015W BRE2 CPS60 Subunit of COMPASS (Set1C) complex; COMPASS methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L peptidyl-amino acid modification, telomere organization, protein alkylation, chromatin organization, histone modification transferase activity, methyltransferase activity nucleus 1570 BY4741 12_1 2 C 5 YLR016C PML1 - Subunit of the RES complex; RES complex is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p RNA splicing, mRNA processing, nucleobase-containing compound transport, nuclear transport molecular_function nucleus, cytoplasm 1571 BY4741 12_1 2 C 6 YLR017W MEU1 S-methyl-5-thioadenosine phosphorylase Methylthioadenosine phosphorylase (MTAP); catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression cellular amino acid metabolic process transferase activity, transferring glycosyl groups, RNA binding, transferase activity, mRNA binding cytoplasm 1572 BY4741 12_1 2 C 7 YLR018C POM34 - Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication protein targeting, cytoskeleton organization, protein complex biogenesis, nuclear transport, nucleus organization structural molecule activity nucleus, endomembrane system, membrane 1573 BY4741 12_1 2 C 8 YLR019W PSR2 - Plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p; PSR2 has a paralog, PSR1, that arose from the whole genome duplication response to osmotic stress phosphatase activity, hydrolase activity membrane, plasma membrane 1574 BY4741 12_1 2 C 9 YLR020C YEH2 sterol esterase Steryl ester hydrolase; catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism; YEH2 has a paralog, YEH1, that arose from the whole genome duplication lipid metabolic process, cell wall organization or biogenesis hydrolase activity membrane, plasma membrane 1575 BY4741 12_1 2 C 10 YLR021W IRC25 DMP2|PBA3|POC3 Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions; upregulates proteasome assembly in response to the unfolded protein response activated by mistargeting of proteins (UPRam) RNA catabolic process, protein complex biogenesis molecular_function other 1576 BY4741 12_1 2 C 11 YLR023C IZH3 - Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity cellular ion homeostasis molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1578 BY4741 12_1 2 C 12 YLR024C UBR2 putative ubiquitin-protein ligase UBR2 Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex response to chemical, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process transferase activity cytoplasm 1579 BY4741 12_1 Incorrect 2 D 1 YLR025W SNF7 ESCRT-III subunit protein SNF7|VPL5|RNS4|VPS32|DID1 One of four subunits of the ESCRT-III complex; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes; ESCRT-III stands for endosomal sorting complex required for transport III response to chemical, vesicle organization, proteolysis involved in cellular protein catabolic process, endosomal transport other endomembrane system, membrane, cytoplasm 1580 BY4741 12_1 2 D 2 YLR027C AAT2 aspartate transaminase AAT2|ASP5 Cytosolic aspartate aminotransferase involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells cellular amino acid metabolic process transferase activity peroxisome, cytoplasm 1582 BY4741 12_1 2 D 3 YLR028C ADE16 bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase ADE16 Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine meiotic cell cycle, sporulation, generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process transferase activity, hydrolase activity cytoplasm 1583 BY4741 12_1 2 D 4 YLR042C - - Cell wall protein of unknown function; localizes to the cytoplasm; deletion improves xylose fermentation in industrially engineered strains; YLL042C is not an essential gene biological_process molecular_function cell wall 2653 BY4741 12_2 2 D 5 YLR043C TRX1 thioredoxin TRX1|LMA1 Cytoplasmic thioredoxin isoenzyme; part of thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; with Trx2p, facilitates mitochondrial import of small Tims Tim9p, Tim10p, Tim13p by maintaining them in reduced form; abundance increases iunder DNA replication stress; TRX1 has a paralog, TRX2, that arose from the whole genome duplication vacuole organization, response to chemical, organelle fusion, response to oxidative stress, Golgi vesicle transport, membrane fusion, organelle inheritance oxidoreductase activity vacuole, mitochondrion, mitochondrial envelope, cytoplasm 2654 BY4741 12_2 2 D 6 YLR044C PDC1 indolepyruvate decarboxylase 1 Major of three pyruvate decarboxylase isozymes; key enzyme in alcoholic fermentation; decarboxylates pyruvate to acetaldehyde; involved in amino acid catabolism; subject to glucose-, ethanol-, and autoregulation; activated by phosphorylation in response to glucose levels; N-terminally propionylated in vivo carbohydrate metabolic process, cofactor metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, cellular amino acid metabolic process, nucleobase-containing small molecule metabolic process lyase activity nucleus, cytoplasm 2655 BY4741 12_2 2 D 7 YLR046C - RLA1 Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; YLR046C has a paralog, RTA1, that arose from the whole genome duplication biological_process molecular_function membrane 2657 BY4741 12_2 2 D 8 YLR047C FRE8 putative ferric-chelate reductase Protein with sequence similarity to iron/copper reductases; involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p cellular ion homeostasis oxidoreductase activity membrane, vacuole, cytoplasm 2658 BY4741 12_2 2 D 9 YLR048W RPS0B ribosomal 40S subunit protein S0B|S2|S0B|YST2|NAB1B Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 cytoplasmic translation, rRNA processing, organelle assembly, nucleobase-containing compound transport, ribosomal small subunit biogenesis, ribosome assembly, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 2659 BY4741 12_2 2 D 10 YLR049C - - Putative protein of unknown function biological_process molecular_function cellular_component 2660 BY4741 12_2 2 D 11 YLR053C - - Putative protein of unknown function biological_process molecular_function cellular_component 2664 BY4741 12_2 2 D 12 YLR054C OSW2 - Protein of unknown function reputedly involved in spore wall assembly meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function membrane, cytoplasm 2665 BY4741 12_2 2 E 1 YLR055C SPT8 SAGA complex subunit SPT8 Subunit of the SAGA transcriptional regulatory complex; not present in SAGA-like complex SLIK/SALSA; required for SAGA-mediated inhibition at some promoters peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification, transcription from RNA polymerase II promoter transcription factor activity, protein binding, transcription factor binding nucleus 2666 BY4741 12_2 2 E 2 YLR056W ERG3 C-5 sterol desaturase|PSO6|SYR1 C-5 sterol desaturase; glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; transcriptionally down-regulated when ergosterol is in excess; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of HRD ubiquitin ligase; mutation is functionally complemented by human SC5D lipid metabolic process oxidoreductase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 2667 BY4741 12_2 2 E 3 YLR057W MNL2 putative mannosidase MNL2 Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene response to chemical, proteolysis involved in cellular protein catabolic process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm 2668 BY4741 12_2 2 E 4 YLR058C SHM2 glycine hydroxymethyltransferase SHM2|SHMT2 Cytosolic serine hydroxymethyltransferase; converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis other transferase activity membrane, site of polarized growth, plasma membrane, cytoplasm 2669 BY4741 12_2 2 E 5 YLR059C REX2 YNT20 3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease rRNA processing, mitochondrion organization nuclease activity, hydrolase activity mitochondrion, cytoplasm 2670 BY4741 12_2 2 E 6 YLR061W RPL22A ribosomal 60S subunit protein L22A|L22e|rp4|l1c|YL31|L22A Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 2672 BY4741 12_2 2 E 7 YLR062C BUD28 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay mitotic cell cycle, cytokinesis molecular_function cellular_component 2673 BY4741 12_2 2 E 8 YLR063W BMT6 25S rRNA (uracil2843-N3)-methyltransferase Methyltransferase required for m3U2843 methylation of the 25S rRNA; S-adenosylmethionine-dependent; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene rRNA processing, RNA modification transferase activity, methyltransferase activity cytoplasm 2674 BY4741 12_2 2 E 9 YLR064W PER33 - Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm 2675 BY4741 12_2 2 E 10 YLR065C ENV10 - Protein proposed to be involved in vacuolar functions; putative role in secretory protein quality control; mutant shows defect in CPY processing; YLR065C is not an essential gene protein maturation molecular_function membrane 2676 BY4741 12_2 2 E 11 YLR067C PET309 - Specific translational activator for the COX1 mRNA; binds to the COX1 mRNA; also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains 12 pentatricopeptide repeats (PPRs) translational initiation, mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis RNA binding, mRNA binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 2678 BY4741 12_2 2 E 12 YLR068W FYV7 - Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 2679 BY4741 12_2 2 F 1 YLR069C MEF1 - Mitochondrial elongation factor involved in translational elongation mitochondrial translation, mitochondrion organization, translational elongation RNA binding, translation factor activity, RNA binding mitochondrion, cytoplasm 2680 BY4741 12_2 2 F 2 YLR070C XYL2 D-xylulose reductase XYL2 Xylitol dehydrogenase; converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect carbohydrate metabolic process oxidoreductase activity cellular_component 2681 BY4741 12_2 2 F 3 YLR072W LAM6 LTC1 Sterol transporter that transfers sterols between membranes; may regulate and coordinate formation of contact sites between organelles; localizes to ER-mitochondrial contact sites in a Tom70p- and Tom71p-dependent manner; mitochondrial localization requires GRAM domain; also localizes to ER-vacuole contact sites, in a Vac8p-dependent manner; has GRAM and StART-like (VASt) domains; one of six StART-like domain-containing proteins in yeast; conserved across eukaryotes lipid transport other cytoplasm 2683 BY4741 12_2 2 F 4 YLR073C RFU1 - Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes other enzyme regulator activity endomembrane system, cytoplasm 2684 BY4741 12_2 2 F 5 YLR074C BUD20 - C2H2-type zinc finger protein required for ribosome assembly; shuttling factor which associates with pre-60S particles in the nucleus, accompanying them to the cytoplasm; cytoplasmic dissociation of Bud20p requires Drg1p; N-terminus harbors a nuclear localization signal (NLS) and a nuclear export signal (NES); cytoplasmic Bud20p is reimported by Kap123-dependent pathway; involved in bud-site selection; diploid mutants display a random budding pattern; similar to human ZNF593 mitotic cell cycle, cytokinesis, ribosomal subunit export from nucleus, nuclear transport other nucleus, cytoplasm 2685 BY4741 12_2 2 F 6 YLR077W FMP25 BCA1 Protein required for assembly of respiratory complex III; mitochondrial inner membrane protein; required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria mitochondrion organization, protein complex biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 2688 BY4741 12_2 2 F 7 YLR079W SIC1 cyclin-dependent protein serine/threonine kinase inhibiting protein SIC1|SDB25 Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylated by Clb5/6-Cdk1 and Cln1/2-Cdk1 kinase which regulate timing of Sic1p degradation; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 mitotic cell cycle, regulation of protein modification process, protein phosphorylation, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm 2690 BY4741 12_2 2 F 8 YLR080W EMP46 - Integral membrane component of ER-derived COPII-coated vesicles; functions in ER to Golgi transport; EMP46 has a paralog, EMP47, that arose from the whole genome duplication Golgi vesicle transport other membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 2691 BY4741 12_2 2 F 9 YLR081W GAL2 galactose permease GAL2 Galactose permease; required for utilization of galactose; also able to transport glucose carbohydrate metabolic process, carbohydrate transport transmembrane transporter activity membrane, plasma membrane 2692 BY4741 12_2 2 F 10 YLR082C SRL2 - Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation other molecular_function nucleus, cytoplasm 2693 BY4741 12_2 2 F 11 YLR083C EMP70 TMN1|p24a Protein with a role in cellular adhesion and filamentous growth; also endosome-to-vacuole sorting; similar to Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments; EMP70 has a paralog, TMN2, that arose from the whole genome duplication invasive growth in response to glucose limitation, cellular ion homeostasis, endosomal transport, pseudohyphal growth molecular_function endomembrane system, vacuole, membrane, cytoplasm 2694 BY4741 12_2 2 F 12 YLR084C RAX2 - N-glycosylated protein; involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p mitotic cell cycle, cytokinesis molecular_function mitochondrion, cell wall, cellular bud, membrane, site of polarized growth, cytoplasm 2695 BY4741 12_2 2 G 1 YLR085C ARP6 - Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A chromatin organization chromatin binding nucleus, chromosome, cytoplasm 2696 BY4741 12_2 2 G 2 YLR087C CSF1 - Protein required for fermentation at low temperature; plays a role in the maturation of secretory proteins; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies protein maturation, generation of precursor metabolites and energy molecular_function mitochondrion, cytoplasm 2698 BY4741 12_2 2 G 3 YLR089C ALT1 alanine transaminase ALT1 Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthesis and catabolism; TOR1-independent role in determining chronological lifespan; expression is induced in the presence of alanine; repression is mediated by Nrg1p; ALT1 has a paralog, ALT2, that arose from the whole genome duplication; Alt2p is catalytically inactive cellular amino acid metabolic process transferase activity mitochondrion, cytoplasm 2700 BY4741 12_2 2 G 4 YLR090W XDJ1 - Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies protein targeting, mitochondrion organization, transmembrane transport, protein folding enzyme regulator activity nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm 2701 BY4741 12_2 2 G 5 YLR091W GEP5 RRG5 Protein of unknown function; required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine mitochondrion organization molecular_function mitochondrion, cytoplasm 2702 BY4741 12_2 2 G 6 YLR092W SUL2 - High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates ion transport transmembrane transporter activity membrane, plasma membrane 2703 BY4741 12_2 2 G 7 YLR093C NYV1 MAM2 v-SNARE component of the vacuolar SNARE complex; involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane vacuole organization, organelle fusion, vesicle organization, membrane fusion other vacuole, membrane, cytoplasm 2704 BY4741 12_2 2 G 8 YLR094C GIS3 - Protein of unknown function signaling molecular_function nucleus, cytoplasm 2705 BY4741 12_2 2 G 9 YLR095C IOC2 - Subunit of the Isw1b complex; exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing; contains a PHD finger motif; other complex members are Isw1p and Ioc4p chromatin organization chromatin binding, DNA binding, ATPase activity, hydrolase activity nucleus 2706 BY4741 12_2 2 G 10 YLR096W KIN2 serine/threonine protein kinase KIN2 Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; KIN2 has a paralog, KIN1, that arose from the whole genome duplication protein phosphorylation, exocytosis kinase activity, transferase activity membrane, plasma membrane 2707 BY4741 12_2 2 G 11 YLR097C HRT3 SCF ubiquitin ligase complex subunit HRT3 Putative SCF-ubiquitin ligase F-box protein; based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies response to chemical, proteolysis involved in cellular protein catabolic process protein binding, bridging, transferase activity other 2708 BY4741 12_2 2 G 12 YLR098C CHA4 SIL3|SIL2 DNA binding transcriptional activator; mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus 2709 BY4741 12_2 2 H 1 YLR099C ICT1 lysophosphatidic acid acyltransferase ICT1 Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication lipid metabolic process transferase activity cellular_component 2710 BY4741 12_2 2 H 3 YLR102C APC9 anaphase promoting complex subunit 9 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition chromatin organization, protein modification by small protein conjugation or removal transferase activity nucleus 2713 BY4741 12_2 2 H 4 YLR104W LCL2 - Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) response to chemical, proteolysis involved in cellular protein catabolic process molecular_function cellular_component 2715 BY4741 12_2 2 H 5 YLR107W REX3 RNA exonuclease RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases rRNA processing nuclease activity, hydrolase activity nucleus, cytoplasm 2718 BY4741 12_2 2 H 6 YLR108C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication biological_process molecular_function nucleus 2719 BY4741 12_2 2 H 7 YLR109W AHP1 thioredoxin peroxidase AHP1|cTPxIII Thiol-specific peroxiredoxin; reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p response to chemical, response to oxidative stress oxidoreductase activity membrane, plasma membrane, cytoplasm 2720 BY4741 12_2 2 H 8 YLR111W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 2722 BY4741 12_2 2 H 9 YLR112W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 2723 BY4741 12_2 2 H 10 YLR113W HOG1 mitogen-activated protein kinase HOG1|SSK3 Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNAPII in osmotic shock; activates CDC28 by stimulating antisense RNA transcription; mediates recruitment/activation of RNAPII at Hot1p-dependent promoters; with Mrc1p defines novel S-phase checkpoint that prevent conflicts between DNA replication and transcription; nuclear form represses pseudohyphal growth; autophosphorylates; protein abundance increases under DNA replication stress response to heat, response to chemical, protein phosphorylation, DNA replication, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, signaling, response to osmotic stress, regulation of cell cycle chromatin binding, kinase activity, transferase activity, signal transducer activity nucleus, cytoplasm 2724 BY4741 12_2 2 H 11 YLR114C AVL9 - Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Golgi vesicle transport molecular_function cellular bud, site of polarized growth, cytoplasm 2725 BY4741 12_2 2 H 12 YLR118C - palmitoyl-(protein) hydrolase|APT1 Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS other hydrolase activity nucleus, cytoplasm 2729 BY4741 12_2 3 A 1 YLR119W SRN2 ESCRT-I subunit protein SRN2|VPL16|VPS37|SRN10 Component of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus protein targeting, proteolysis involved in cellular protein catabolic process other membrane, endomembrane system, cytoplasm 2730 BY4741 12_2 3 A 2 YLR120C YPS1 YAP3 Aspartic protease; hyperglycosylated member of the yapsin family of proteases, attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor; involved in nutrient limitation-induced cleavage of the extracellular inhibitory domain of signaling mucin Msb2p, resulting in activation of the filamentous growth MAPK pathway; involved with other yapsins in the cell wall integrity response; role in KEX2-independent processing of the alpha factor precursor protein maturation, conjugation, cell wall organization or biogenesis, signaling peptidase activity, hydrolase activity membrane, plasma membrane 2731 BY4741 12_2 3 A 4 YLR121C YPS3 YPS4 Aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor cell wall organization or biogenesis peptidase activity, hydrolase activity membrane, plasma membrane 2732 BY4741 12_2 3 A 5 YLR122C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C biological_process molecular_function cellular_component 2733 BY4741 12_2 3 A 6 YLR123C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif biological_process molecular_function cellular_component 2734 BY4741 12_2 3 A 7 YLR124W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 2735 BY4741 12_2 3 A 8 YLR125W - - Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene biological_process molecular_function cellular_component 2736 BY4741 12_2 Incorrect 3 A 9 YML089C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage biological_process molecular_function cellular_component 481 BY4730 13_1 3 A 10 YML088W UFO1 SCF ubiquitin ligase complex subunit UFO1 F-box receptor protein; subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation response to chemical, cellular response to DNA damage stimulus, proteolysis involved in cellular protein catabolic process protein binding, bridging, transferase activity nucleus, cytoplasm 482 BY4730 13_1 3 A 11 YML087C AIM33 - Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication biological_process molecular_function cellular_component 483 BY4730 13_1 3 A 12 YML086C ALO1 D-arabinono-1,4-lactone oxidase D-Arabinono-1,4-lactone oxidase; catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress response to chemical, response to oxidative stress oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 484 BY4730 13_1 3 B 1 YML084W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 486 BY4730 13_1 3 B 2 YML083C - - Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions biological_process molecular_function cellular_component 487 BY4730 13_1 3 B 3 YML082W - putative cystathionine gamma-synthase Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; not an essential gene; YML082W has a paralog, STR2, that arose from the whole genome duplication other lyase activity nucleus, cytoplasm 488 BY4730 13_1 3 B 4 YML081W TDA9 AAF1 Transcription factor that regulates acetate production; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication monocarboxylic acid metabolic process DNA binding nucleus 489 BY4730 13_1 3 B 5 YML080W DUS1 - Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 tRNA processing, RNA modification oxidoreductase activity nucleus 490 BY4730 13_1 3 B 6 YML079W - - Non-essential protein of unknown function; has structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm biological_process molecular_function nucleus, cytoplasm 491 BY4730 13_1 3 B 7 YML078W CPR3 peptidylprolyl isomerase CPR3|CYP3 Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria protein folding isomerase activity mitochondrion, cytoplasm 492 BY4730 13_1 3 B 8 YML063W RPS1B ribosomal 40S subunit protein S1B|S1e|rp10B|S1B|RP10B|PLC2 Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 507 BY4741 13_1 3 B 9 YML062C MFT1 MFT52 Subunit of the THO complex; THO is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance nucleobase-containing compound transport, DNA recombination, nuclear transport, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation structural molecule activity nucleus, chromosome 508 BY4741 13_1 3 B 10 YML061C PIF1 DNA helicase PIF1|TST1 DNA helicase, potent G-quadruplex DNA binder/unwinder; possesses strand annealing activity; promotes DNA synthesis during break-induced replication; important for crossover recombination; translation from different start sites produces mitochondrial and nuclear forms; nuclear form is a catalytic inhibitor of telomerase; mitochondrial form involved in DNA repair and recombination; mutations affect Zn, Fe homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells DNA replication, DNA recombination, DNA repair, telomere organization, mitochondrion organization, regulation of organelle organization, cellular response to DNA damage stimulus, regulation of DNA metabolic process hydrolase activity, enzyme regulator activity, DNA binding, helicase activity nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm 509 BY4741 13_1 3 B 11 YML060W OGG1 8-oxoguanine glycosylase OGG1 Nuclear and mitochondrial glycosylase/lyase; specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance DNA repair, telomere organization, DNA replication, cellular response to DNA damage stimulus hydrolase activity, hydrolase activity, acting on glycosyl bonds, lyase activity, nuclease activity nucleus, chromosome, mitochondrion, cytoplasm 510 BY4741 13_1 3 B 12 YML059C NTE1 - Serine esterase; homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes lipid metabolic process hydrolase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 511 BY4741 13_1 3 C 1 YML058W SML1 ribonucleotide reductase inhibiting protein SML1 Ribonucleotide reductase inhibitor; involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase; SML1 has a paralog, DIF1, that arose from the whole genome duplication mitochondrion organization, cellular response to DNA damage stimulus enzyme regulator activity nucleus, cytoplasm 512 BY4741 13_1 3 C 2 YML057W CMP2 calcineurin catalytic subunit A|CNA2 Calcineurin A; one isoform (the other is Cna1p) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1; regulates the function of Aly1p alpha-arrestin; CMP2 has a paralog, CNA1, that arose from the whole genome duplication response to chemical, cellular ion homeostasis, conjugation, signaling phosphatase activity, hydrolase activity cytoplasm 513 BY4741 13_1 3 C 3 YML058C-A cytoplasm cytoplasm cytoplasm - - - 514 BY4741 13_1 3 C 4 YML056C IMD4 IMP dehydrogenase IMD4 Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in the de novo synthesis of GTP; member of a four-gene family in S. cerevisiae, constitutively expressed; IMD4 has a paralog, IMD3, that arose from the whole genome duplication biological_process RNA binding, mRNA binding, oxidoreductase activity cytoplasm 515 BY4741 13_1 3 C 5 YML055W SPC2 signal peptidase complex subunit SPC2|SPY1 Subunit of signal peptidase complex; complex catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25; other members of the complex are Spc1p, Spc1p, and Sec11p protein targeting, protein maturation peptidase activity, hydrolase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 516 BY4741 13_1 3 C 6 YML054C CYB2 FCB2 Cytochrome b2 (L-lactate cytochrome-c oxidoreductase); component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions monocarboxylic acid metabolic process oxidoreductase activity mitochondrial envelope, mitochondrion, cytoplasm 517 BY4741 13_1 3 C 7 YML053C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene biological_process molecular_function nucleus, cytoplasm 518 BY4741 13_1 3 C 8 YML052W SUR7 - Plasma membrane protein, component of eisosomes; long-lived protein that remains stable in eisosomes of mother cells while other eisosome proteins, Pil1p and Lsp1p, turn over; may function to anchor the eisosome in place; sporulation and plasma membrane sphingolipid content are altered in mutants; localizes to furrow-like invaginations (MCC patches) meiotic cell cycle, sporulation, endocytosis molecular_function cell cortex, mitochondrion, membrane, cytoplasm, plasma membrane 519 BY4741 13_1 3 C 9 YML051W GAL80 transcription regulator GAL80 Transcriptional regulator involved in the repression of GAL genes; involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding carbohydrate metabolic process, transcription from RNA polymerase II promoter transcription factor binding, enzyme regulator activity nucleus, cytoplasm 520 BY4741 13_1 3 C 10 YML050W AIM32 - Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular_component 521 BY4741 13_1 3 C 11 YML048W GSF2 ECM6 Endoplasmic reticulum (ER) localized integral membrane protein; may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression protein folding unfolded protein binding membrane, endomembrane system, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm 523 BY4741 13_1 3 C 12 YML048W-A membrane, endomembrane system, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm membrane, endomembrane system, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm membrane, endomembrane system, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm - - - 524 BY4741 13_1 3 D 1 YML037C - - Putative protein of unknown function; has some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene biological_process molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm 534 BY4741 13_2 3 D 2 YML035C AMD1 AMP deaminase|AMD3 AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools nucleobase-containing small molecule metabolic process hydrolase activity cytoplasm 536 BY4741 13_2 3 D 3 YML034W SRC1 HEH1|YML033W Inner nuclear membrane protein; highly enriched at telomeres and subtelomeric regions; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance; SEC1 has a paralog, HEH2, that arose from the whole genome duplication mitotic cell cycle, organelle fission, chromosome segregation molecular_function nucleus, endomembrane system, chromosome 537 BY4741 13_2 3 D 4 YML035C-A nucleus, endomembrane system, chromosome nucleus, endomembrane system, chromosome nucleus, endomembrane system, chromosome - - - 538 BY4741 13_2 3 D 5 YML033W - - - - - - 539 BY4741 13_2 3 D 6 YML032C RAD52 recombinase RAD52 Protein that stimulates strand exchange; stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis and UV induced sister chromatid recombination meiotic cell cycle, DNA repair, telomere organization, organelle fission, cellular response to DNA damage stimulus, DNA recombination other nucleus, chromosome 540 BY4741 13_2 3 D 7 YML030W RCF1 AIM31 Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast mitochondrion organization, protein complex biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 543 BY4741 13_2 3 D 8 YML029W USA1 - Scaffold subunit of the Hrd1p ubiquitin ligase; also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p response to chemical, RNA splicing, proteolysis involved in cellular protein catabolic process, protein complex biogenesis, mRNA processing structural molecule activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 544 BY4741 13_2 3 D 9 YML028W TSA1 thioredoxin peroxidase TSA1|cTPxI|TPX1|ZRG14 Thioredoxin peroxidase; acts as both ribosome-associated and free cytoplasmic antioxidant; self-associates to form high-molecular weight chaperone complex under oxidative stress; chaperone activity essential for growth in zinc deficiency; required for telomere length maintenance; protein abundance increases, forms cytoplasmic foci during DNA replication stress; TSA1 has a paralog, TSA2, that arose from the whole genome duplication response to chemical, protein folding, cellular response to DNA damage stimulus, response to oxidative stress unfolded protein binding, oxidoreductase activity cytoplasm 545 BY4741 13_2 3 D 10 YML026C RPS18B ribosomal 40S subunit protein S18B|S18B|S13 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation, rRNA processing, nucleobase-containing compound transport, ribosomal small subunit biogenesis, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm 547 BY4741 13_2 3 D 11 YML024W RPS17A ribosomal 40S subunit protein S17A|S17e|rp51A|S17A|RPL51A|RP51A Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication cytoplasmic translation, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 549 BY4741 13_2 3 D 12 YML020W - - Putative protein of unknown function biological_process molecular_function cellular_component 553 BY4741 13_2 3 E 1 YML019W OST6 dolichyl-diphosphooligosaccharide--protein glycotransferase Subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p peptidyl-amino acid modification, carbohydrate metabolic process, protein complex biogenesis, protein glycosylation molecular_function endomembrane system, membrane, endoplasmic reticulum, cytoplasm 554 BY4741 13_2 3 E 2 YML018C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication biological_process molecular_function membrane, vacuole, cytoplasm 555 BY4741 13_2 3 E 3 YML017W PSP2 MRS15 Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing biological_process RNA binding, mRNA binding cytoplasm 556 BY4741 13_2 3 E 4 YML016C PPZ1 - Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance cellular ion homeostasis, protein dephosphorylation phosphatase activity, hydrolase activity nucleus, membrane, plasma membrane, cytoplasm 557 BY4741 13_2 3 E 5 YML014W TRM9 tRNA (carboxymethyluridine(34)-5-O)-methyltransferase|KTI1 tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses response to chemical, tRNA processing, RNA modification transferase activity, methyltransferase activity nucleus, cytoplasm 559 BY4741 13_2 3 E 6 YML013W UBX2 SEL1 Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis response to chemical, proteolysis involved in cellular protein catabolic process protein binding, bridging endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm, plasma membrane 560 BY4741 13_2 3 E 7 YML013C-A endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm, plasma membrane - - - 561 BY4741 13_2 3 E 8 YML012W ERV25 - Member of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, Golgi vesicle transport molecular_function cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm 562 BY4741 13_2 3 E 9 YML011C RAD33 - Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus DNA repair, cellular response to DNA damage stimulus molecular_function nucleus 563 BY4741 13_2 3 E 10 YML009c nucleus nucleus nucleus - - - 567 BY4741 13_2 3 E 11 YML008C ERG6 sterol 24-C-methyltransferase|VID1|SED6|LIS1|ISE1 Delta(24)-sterol C-methyltransferase; converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to lipid particles, the plasma membrane-associated endoplasmic reticulum, and the mitochondrial outer membrane lipid metabolic process transferase activity, methyltransferase activity endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 568 BY4741 13_2 3 E 12 YML007W YAP1 PDR4|DNA-binding transcription factor YAP1|SNQ3|PAR1 Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; relative distribution to the nucleus increases upon DNA replication stress; YAP1 has a paralog, CAD1, that arose from the whole genome duplication response to heat, response to chemical, transcription from RNA polymerase II promoter, response to oxidative stress nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 569 BY4741 13_2 3 F 1 YML006C GIS4 - CAAX box containing protein of unknown function; proposed to be involved in the RAS/cAMP signaling pathway cellular ion homeostasis, signaling molecular_function membrane, plasma membrane 570 BY4741 13_2 3 F 2 YML005W TRM12 TYW2 S-adenosylmethionine-dependent methyltransferase; required for wybutosine formation in phenylalanine-accepting tRNA; member of the seven beta-strand family tRNA processing, RNA modification transferase activity, methyltransferase activity cytoplasm 571 BY4741 13_2 3 F 3 YML004C GLO1 lactoylglutathione lyase GLO1 Monomeric glyoxalase I; catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress monocarboxylic acid metabolic process lyase activity nucleus, cytoplasm 572 BY4741 13_2 3 F 4 YML003W - - Putative protein of unknown function biological_process molecular_function cellular_component 573 BY4741 13_2 3 F 5 YML002W - - Putative protein of unknown function; expression induced by heat and by calcium shortage biological_process molecular_function cellular_component 574 BY4741 13_2 3 F 6 YML001W YPT7 Rab family GTPase YPT7|VAM4|AST4 Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs); interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 vacuole organization, organelle fusion, protein targeting, regulation of organelle organization, membrane fusion, membrane invagination, endosomal transport, organelle inheritance, endocytosis GTPase activity, hydrolase activity vacuole, mitochondrion, endomembrane system, membrane, mitochondrial envelope, cytoplasm 575 BY4741 13_2 3 F 7 YMR002W MIX17 MIC17 Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress generation of precursor metabolites and energy, cellular respiration molecular_function nucleus, mitochondrial envelope, mitochondrion, cytoplasm 577 BY4741 13_2 3 F 8 YMR003W AIM34 - Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 578 BY4741 13_2 3 F 9 YMR006C PLB2 - Phospholipase B (lysophospholipase) involved in lipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine lipid metabolic process hydrolase activity cell wall, extracellular region 581 BY4741 13_2 3 F 10 YMR007W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 582 BY4741 13_2 3 F 11 YMR008C PLB1 - Phospholipase B (lysophospholipase) involved in lipid metabolism; required for efficient acyl chain remodeling of newly synthesized phosphatidylethanolamine-derived phosphatidylcholine; required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol; PLB1 has a paralog, PLB3, that arose from the whole genome duplication lipid metabolic process hydrolase activity membrane, endomembrane system, endoplasmic reticulum, plasma membrane, cytoplasm 583 BY4741 13_2 3 F 12 YMR009W ADI1 acireductone dioxygenase (Ni2+-requiring) Acireductone dioxygenease involved in methionine salvage pathway; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions; human ortholog ADI1 can complement yeast adi1 mutant cellular amino acid metabolic process oxidoreductase activity nucleus, cytoplasm 584 BY4741 13_2 3 G 1 YMR010W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 biological_process molecular_function membrane, cytoplasm 585 BY4741 13_2 3 G 2 YMR011W HXT2 hexose transporter HXT2 High-affinity glucose transporter of the major facilitator superfamily; expression is induced by low levels of glucose and repressed by high levels of glucose carbohydrate transport transmembrane transporter activity membrane, plasma membrane 586 BY4741 13_2 3 G 3 YMR012W CLU1 translation initiation factor 3 subunit CLU1|TIF31 Subunit of the eukaryotic translation initiation factor 3 (eIF3); component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation; can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant; eIF3 is also involved in programmed stop codon readthrough cytoplasmic translation, translational initiation RNA binding, translation factor activity, RNA binding, mRNA binding cytoplasm 587 BY4741 13_2 3 G 4 YMR014W BUD22 - Protein required for rRNA maturation and ribosomal subunit biogenesis; required for 18S rRNA maturation; also required for small ribosomal subunit biogenesis; cosediments with pre-ribosomal particles; mutation decreases efficiency of +1 Ty1 frameshifting and transposition, and affects budding pattern rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 589 BY4741 13_2 3 G 5 YMR015C ERG5 C-22 sterol desaturase|CYP61 C-22 sterol desaturase; a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs lipid metabolic process oxidoreductase activity endomembrane system, endoplasmic reticulum, cytoplasm 590 BY4741 13_2 3 G 6 YMR016C SOK2 - Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; relocalizes to the cytosol in response to hypoxia; SOK2 has a paralog, PHD1, that arose from the whole genome duplication pseudohyphal growth nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 591 BY4741 13_2 3 G 7 YMR017W SPO20 DBI9 Meiosis-specific subunit of the t-SNARE complex; required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog meiotic cell cycle, sporulation other membrane, cytoplasm 592 BY4741 13_2 3 G 8 YMR018W - - Putative protein of unknown function with similarity to human PEX5Rp; transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene; PEX5Rp is also known as peroxin protein 5 related protein biological_process molecular_function cellular_component 593 BY4741 13_2 3 G 9 YMR019W STB4 - Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; biological_process DNA binding nucleus 594 BY4741 13_2 3 G 10 YMR020W FMS1 polyamine oxidase Polyamine oxidase; converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis cofactor metabolic process, vitamin metabolic process, monocarboxylic acid metabolic process oxidoreductase activity cytoplasm 595 BY4741 13_2 3 G 11 YMR021C MAC1 CUA1 Copper-sensing transcription factor; involved in regulation of genes required for high affinity copper transport; required for regulation of yeast copper genes in response to DNA-damaging agents; undergoes changes in redox state in response to changing levels of copper or MMS cellular ion homeostasis, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 596 BY4741 13_2 3 G 12 YMR022W UBC7 E2 ubiquitin-conjugating protein UBC7|DER2|QRI8 Ubiquitin conjugating enzyme; involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly response to chemical, chromatin organization, proteolysis involved in cellular protein catabolic process, cell wall organization or biogenesis transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 597 BY4741 13_2 3 H 1 YMR023C MSS1 PET53 Mitochondrial protein; forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 tRNA processing, mitochondrion organization, RNA modification ion binding membrane, mitochondrial envelope, mitochondrion, cytoplasm 598 BY4741 13_2 3 H 3 YMR024W MRPL3 mitochondrial 54S ribosomal protein YmL3|YmL3 Mitochondrial ribosomal protein of the large subunit; located in close proximity to the polypeptide exit channel of the ribosome; mutations in human homolog MRPL44 cause childhood cardiomyopathy; human MRPL44 deficiency results in inefficient assembly of the mitochondrial ribosome, and in tissue-specific respiratory chain deficiency, manifesting as either Complex I+Complex IV or Complex IV deficiency, depending on a cell type mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 599 BY4741 13_2 3 H 4 YMR025W CSI1 - Subunit of the Cop9 signalosome; which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome response to chemical, protein modification by small protein conjugation or removal, conjugation, signaling molecular_function nucleus 600 BY4741 13_2 3 H 5 YMR026C PEX12 ubiquitin-protein ligase peroxin 12|PAS11 C3HC4-type RING-finger peroxin and E3 ubiquitin ligase; required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder protein targeting, protein modification by small protein conjugation or removal, transmembrane transport, peroxisome organization transferase activity peroxisome, membrane, cytoplasm 601 BY4741 13_2 3 H 6 YMR027W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene biological_process molecular_function nucleus, cytoplasm 602 BY4741 13_2 3 H 7 YMR029C FAR8 - Protein involved in recovery from arrest in response to pheromone; acts in a cell cycle arrest recovery pathway independent from Far1p; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p response to chemical, conjugation, signaling molecular_function endomembrane system, endoplasmic reticulum, cytoplasm 604 BY4741 13_2 3 H 8 YMR030W RSF1 - Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes mitochondrion organization, generation of precursor metabolites and energy, cellular respiration, transcription from RNA polymerase II promoter, signaling molecular_function nucleus, mitochondrion, cytoplasm 605 BY4741 13_2 3 H 9 YMR031W-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C biological_process molecular_function cellular_component 606 BY4741 13_2 3 H 10 YMR031C EIS1 - Component of the eisosome required for proper eisosome assembly; similar to Uso1p; authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress; EIS1 has a paralog, YKL050C, that arose from the whole genome duplication other molecular_function membrane, mitochondrion, plasma membrane, cytoplasm 607 BY4741 13_2 3 H 11 YMR032W HOF1 formin-binding protein HOF1|CYK2 Protein that regulates actin cytoskeleton organization; required for cytokinesis, actin cable organization, and secretory vesicle trafficking; localized to bud neck; phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; contains an SH3 domain; N terminus controls cell size and levels of actin cables, while C terminus controls actin cable organization via direct regulation of the formin Bnr1p mitotic cell cycle, cytoskeleton organization, regulation of organelle organization, cytokinesis, cellular response to DNA damage stimulus cytoskeletal protein binding cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 608 BY4741 13_2 3 H 12 YMR034C RCH1 - Putative transporter; localizes to the plasma membrane in response to high levels of extracellular calcium; member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene biological_process other membrane 610 BY4741 13_2 4 A 1 YMR035W IMP2 - Catalytic subunit of mitochondrial inner membrane peptidase complex; required for maturation of mitochondrial proteins of the intermembrane space; complex contains two catalytic subunits (Imp1p and Imp2p that differ in substrate specificity), and Som1p protein targeting, mitochondrion organization, protein maturation peptidase activity, hydrolase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 611 BY4741 13_2 4 A 2 YMR036C MIH1 putative tyrosine protein phosphatase MIH1 Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 mitotic cell cycle, protein phosphorylation, regulation of protein modification process, regulation of cell cycle phosphatase activity, hydrolase activity nucleus, cytoplasm 612 BY4741 13_2 4 A 3 YMR039C SUB1 chromatin-binding transcription coactivator SUB1|TSP1 Transcriptional regulator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in nonhomologous end-joining (NHEJ) of ds breaks in plasmid DNA, but not chromosomal DNA; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; negatively regulates sporulation; protein abundance increases in response to DNA replication stress; functionally complemented by human SUB1 (PC4) meiotic cell cycle, organelle fission, transcription from RNA polymerase III promoter, DNA-templated transcription, termination, DNA-templated transcription, initiation, transcription from RNA polymerase II promoter, response to osmotic stress, DNA-templated transcription, elongation, DNA repair, sporulation, regulation of organelle organization, cellular response to DNA damage stimulus, regulation of cell cycle chromatin binding, transcription factor activity, protein binding nucleus 615 BY4741 13_2 4 A 5 YMR040W YET2 - Protein of unknown function that may interact with ribosomes; based on co-purification experiments; homolog of human BAP31 protein; YET2 has a paralog, YET1, that arose from the whole genome duplication biological_process molecular_function membrane, ribosome, cytoplasm 616 BY4741 13_2 4 A 6 YMR041C ARA2 D-arabinose 1-dehydrogenase (NAD(P)(+)) ARA2 NAD-dependent arabinose dehydrogenase; involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase other oxidoreductase activity cellular_component 617 BY4741 13_2 4 A 7 YMR042W ARG80 ARGRI|ARGR1 Transcription factor involved in regulating arginine-responsive genes; acts with Arg81p and Arg82p transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus 618 BY4741 13_2 4 A 8 YMR044W IOC4 - Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing chromatin organization chromatin binding, DNA binding, ATPase activity, hydrolase activity nucleus 620 BY4741 13_2 4 A 9 YMR140W SIP5 - Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress response to starvation molecular_function cytoplasm 721 BY4741 13_4 4 A 10 YMR141C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 722 BY4741 13_4 4 A 11 YMR143W RPS16A ribosomal 40S subunit protein S16A|S9|rp61R|S16A Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 724 BY4741 13_4 4 A 12 YMR144W - - Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene biological_process molecular_function nucleus 725 BY4741 13_4 4 B 1 YMR145C NDE1 NADH-ubiquinone reductase (H(+)-translocating) NDE1|NDH1 Mitochondrial external NADH dehydrogenase; type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain; NDE1 has a paralog, NDE2, that arose from the whole genome duplication carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 726 BY4741 13_4 4 B 2 YMR147W - - Putative protein of unknown function biological_process molecular_function cellular_component 728 BY4741 13_4 4 B 3 YMR148W OSW5 - Protein of unknown function with possible role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion biological_process molecular_function membrane 729 BY4741 13_4 4 B 4 YMR151W YIM2 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 biological_process molecular_function cellular_component 731 BY4741 13_4 4 B 5 YMR150C IMP1 endopeptidase catalytic subunit IMP1|PET-TS2858 Catalytic subunit of mitochondrial inner membrane peptidase complex; required for maturation of mitochondrial proteins of the intermembrane space; complex contains two catalytic subunits (Imp1p and Imp2p that differ in substrate specificty) and Som1p protein targeting, mitochondrion organization, protein maturation peptidase activity, hydrolase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 732 BY4741 13_4 4 B 6 YMR152W YIM1 - Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; may have a role in lipid metabolism, based on localization to lipid droplets; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress cellular response to DNA damage stimulus molecular_function mitochondrion, cytoplasm 733 BY4741 13_4 4 B 7 YMR153W NUP53 FG-nucleoporin NUP53 FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication peptidyl-amino acid modification, mitotic cell cycle, organelle fission, protein targeting, regulation of organelle organization, regulation of protein modification process, protein modification by small protein conjugation or removal, nuclear transport, nucleus organization, regulation of cell cycle ion binding, lipid binding, DNA binding nucleus, endomembrane system, membrane 734 BY4741 13_4 4 B 8 YMR153C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 3' end of verified gene NUP53/YMR153W biological_process molecular_function cellular_component 735 BY4741 13_4 4 B 9 YMR155W - - Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen biological_process molecular_function membrane 737 BY4741 13_4 4 B 10 YMR156C TPP1 - DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3'-phosphatase DNA repair, cellular response to DNA damage stimulus phosphatase activity, DNA binding, hydrolase activity nucleus 738 BY4741 13_4 4 B 11 YMR157C AIM36 FMP39 Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 739 BY4741 13_4 4 B 12 YMR158W-A mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - 741 BY4741 13_4 4 C 1 YMR159C ATG16 APG15|APG16|CVT11|SAP18 Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which binds to membranes and localizes to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress protein targeting, mitochondrion organization, membrane invagination, protein lipidation protein binding, bridging, transferase activity nucleus, cytoplasm 742 BY4741 13_4 4 C 2 YMR161W HLJ1 type I HSP40 co-chaperone HLJ1 Co-chaperone for Hsp40p; anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ response to chemical, proteolysis involved in cellular protein catabolic process enzyme regulator activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 744 BY4741 13_4 4 C 3 YMR162C DNF3 aminophospholipid-translocating P4-type ATPase DNF3 Trans-golgi network aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to the maintenance of membrane lipid asymmetry in post-Golgi secretory vesicles; role in protein trafficking between the Golgi and endosomal system; localizes to the trans-Golgi network; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone response to chemical, ion transport, lipid transport, conjugation hydrolase activity, ATPase activity endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, site of polarized growth, Golgi apparatus, cytoplasm, plasma membrane 745 BY4741 13_4 4 C 4 YMR163C INP2 - Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene organelle inheritance, peroxisome organization other peroxisome, membrane, cytoplasm 746 BY4741 13_4 4 C 5 YMR164C MSS11 - Transcription factor; involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals; forms a heterodimer with Flo8p that interacts with the Swi/Snf complex during transcriptional activation of FLO1, FLO11, and STA1 carbohydrate metabolic process, invasive growth in response to glucose limitation, pseudohyphal growth, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity nucleus 747 BY4741 13_4 4 C 6 YMR166C MME1 - Transporter of the mitochondrial inner membrane that exports magnesium; involved in mitochondrial Mg2+ homeostasis; has similarity to human mitochondrial ATP-Mg/Pi carriers transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 749 BY4741 13_4 4 C 7 YMR167W MLH1 mismatch repair ATPase MLH1|PMS2 Protein required for mismatch repair in mitosis and meiosis; also required for crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer meiotic cell cycle, DNA repair, organelle fission, cellular response to DNA damage stimulus, DNA recombination hydrolase activity, DNA binding, ion binding, ATPase activity nucleus, mitochondrion, cytoplasm 750 BY4741 13_4 4 C 8 YMR169C ALD3 aldehyde dehydrogenase (NAD(+)) ALD3 Cytoplasmic aldehyde dehydrogenase; involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose cellular amino acid metabolic process oxidoreductase activity cytoplasm 752 BY4741 13_4 4 C 9 YMR170C ALD2 aldehyde dehydrogenase (NAD(+)) ALD2 Cytoplasmic aldehyde dehydrogenase; involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p cellular amino acid metabolic process oxidoreductase activity cytoplasm 753 BY4741 13_4 4 C 10 YMR172C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 3' end of ORF HOT1/YMR172W biological_process molecular_function cellular_component 756 BY4741 13_4 4 C 11 YMR173W-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W biological_process molecular_function membrane 758 BY4741 13_4 4 C 12 YMR174C PAI3 IA3 Cytoplasmic proteinase A (Pep4p) inhibitor; dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact other enzyme regulator activity nucleus, cytoplasm 759 BY4741 13_4 4 D 1 YMR175W SIP18 - Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress; SIP18 has a paralog, GRE1, that arose from the whole genome duplication response to chemical ion binding, lipid binding cytoplasm 760 BY4741 13_4 4 D 2 YMR176W ECM5 - Subunit of the Snt2C complex; physically associates with Snt2p and Rpd3p; along with Snt2p, recruits Rpd3p to a small number of promoters; also colocalizes with Snt2p, independently of Rpd3p, to promoters of stress response genes in response to oxidative stress; contains ATP/GTP-binding site motif A; null mutant exhibits increased cellular volume, large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress response to chemical, response to oxidative stress molecular_function nucleus, chromosome, cytoplasm 761 BY4741 13_4 4 D 3 YMR177W MMT1 MFT1 Putative metal transporter involved in mitochondrial iron accumulation; MMT1 has a paralog, MMT2, that arose from the whole genome duplication cellular ion homeostasis molecular_function membrane, mitochondrion, cytoplasm 762 BY4741 13_4 4 D 4 YMR178W - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus, cytoplasm 763 BY4741 13_4 4 D 5 YMR179W SPT21 - Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress chromatin organization, transcription from RNA polymerase II promoter molecular_function nucleus 764 BY4741 13_4 4 D 6 YMR180C CTL1 polynucleotide 5'-phosphatase|CTH1 RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm; CTL1 has a paralog, CET1, that arose from the whole genome duplication biological_process phosphatase activity, hydrolase activity nucleus, cytoplasm 765 BY4741 13_4 4 D 7 YMR182C RGM1 - Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y' telomeric elements and subtelomeric COS genes; relocalizes to the cytosol in response to hypoxia; RGM1 has a paralog, USV1, that arose from the whole genome duplication transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, chromosome, cytoplasm 767 BY4741 13_4 4 D 8 YMR183C SSO2 - Plasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p; SSO2 has a paralog, SSO1, that arose from the whole genome duplication meiotic cell cycle, sporulation, organelle fusion, vesicle organization, membrane fusion, Golgi vesicle transport lipid binding, ion binding endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane 768 BY4741 13_4 4 D 9 YMR184W ADD37 - Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress response to chemical, proteolysis involved in cellular protein catabolic process molecular_function cytoplasm 769 BY4741 13_4 4 D 10 YMR186W HSC82 Hsp90 family chaperone HSC82|HSP90 Cytoplasmic chaperone of the Hsp90 family; plays a role in determining prion variants; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication response to heat, telomere organization, protein complex biogenesis, protein folding hydrolase activity, unfolded protein binding, ATPase activity mitochondrion, membrane, cytoplasm, plasma membrane 771 BY4741 13_4 4 D 11 YMR187C - - Putative protein of unknown function; YMR187C is not an essential gene biological_process molecular_function membrane 772 BY4741 13_4 4 D 12 YMR188C MRPS17 mitochondrial 37S ribosomal protein MRPS17 Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 773 BY4741 13_4 4 E 1 YMR189W GCV2 glycine decarboxylase subunit P|GSD2 P subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 774 BY4741 13_4 4 E 2 YMR190C SGS1 ATP-dependent DNA helicase SGS1 RecQ family nucleolar DNA helicase; role in genome integrity maintenance, chromosome synapsis, meiotic joint molecule/crossover formation; stimulates activity of Top3p; rapidly lost in response to rapamycin in Rrd1p-dependent manner; forms nuclear foci upon DNA replication stress; yeast SGS1 complements mutations in human homolog BLM implicated in Bloom syndrome; also similar to human WRN implicated in Werner syndrome; human BLM and WRN can each complement yeast null mutant mitotic cell cycle, meiotic cell cycle, organelle fission, DNA replication, DNA recombination, DNA repair, telomere organization, regulation of organelle organization, cellular response to DNA damage stimulus, chromosome segregation, regulation of DNA metabolic process, regulation of cell cycle hydrolase activity, helicase activity, ATPase activity nucleus, chromosome, nucleolus 775 BY4741 13_4 4 E 3 YMR191W SPG5 - Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources protein complex biogenesis other cellular_component 776 BY4741 13_4 4 E 4 YMR192W GYL1 APP2 Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress; GYL1 has a paralog, GYP5, that arose from the whole genome duplication regulation of transport, Golgi vesicle transport, exocytosis other endomembrane system, mitochondrion, cellular bud, cytoplasmic membrane-bounded vesicle, membrane, site of polarized growth, Golgi apparatus, cytoplasm, plasma membrane 777 BY4741 13_4 4 E 5 YMR193W MRPL24 mitochondrial 54S ribosomal protein YmL24/YmL14|YmL14|YmL24|MRPL14 Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 778 BY4741 13_4 4 E 6 YMR194W RPL36A ribosomal 60S subunit protein L36A|L36e|YL39|L39|L36A|RPL39B Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 779 BY4741 13_4 4 E 7 YMR193C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 780 BY4741 13_4 4 E 8 YMR195W ICY1 - Protein of unknown function; required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation; ICY1 has a paralog, ICY2, that arose from the whole genome duplication biological_process molecular_function membrane, vacuole, cytoplasm 781 BY4741 13_4 4 E 9 YMR196W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene biological_process molecular_function cytoplasm 782 BY4741 13_4 4 E 10 YMR198W CIK1 - Kinesin-associated protein; required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; locus encodes a long and short transcript with differing functions; CIK1 has a paralog, VIK1, that arose from the whole genome duplication mitotic cell cycle, meiotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, organelle fusion, chromosome segregation, conjugation, regulation of cell cycle, nucleus organization hydrolase activity, ATPase activity cytoskeleton, microtubule organizing center, cytoplasm 784 BY4741 13_4 4 E 11 YMR199W CLN1 cyclin CLN1 G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN1 has a paralog, CLN2, that arose from the whole genome duplication; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1 regulation of protein modification process, protein phosphorylation, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm 785 BY4741 13_4 4 E 12 YMR201C RAD14 - Protein that recognizes and binds damaged DNA during NER; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein; NER stands for nucleotide excision repair DNA repair, cellular response to DNA damage stimulus DNA binding, ion binding nucleus 787 BY4741 13_4 4 F 1 YMR202W ERG2 C-8 sterol isomerase ERG2|END11 C-8 sterol isomerase; catalyzes isomerization of delta-8 double bond to delta-7 position at an intermediate step in ergosterol biosynthesis; transcriptionally down-regulated when ergosterol is in excess; mutation is functionally complemented by human EBP lipid metabolic process isomerase activity endomembrane system, endoplasmic reticulum, cytoplasm 788 BY4741 13_4 4 F 2 YMR204C INP1 - Peripheral membrane protein of peroxisomes; involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane organelle inheritance, peroxisome organization protein binding, bridging peroxisome, membrane, cytoplasm 790 BY4741 13_4 4 F 3 YMR205C PFK2 6-phosphofructokinase subunit beta Beta subunit of heterooctameric phosphofructokinase; involved in glycolysis; indispensable for anaerobic growth; activated by fructose-2,6-bisphosphate and AMP; mutation inhibits glucose induction of cell cycle-related genes carbohydrate metabolic process, cofactor metabolic process, ion transport, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, nucleobase-containing small molecule metabolic process RNA binding, kinase activity, transferase activity, mRNA binding mitochondrion, cytoplasm 791 BY4741 13_4 4 F 4 YMR206W - - Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication biological_process molecular_function cellular_component 792 BY4741 13_4 4 F 5 YMR207C HFA1 acetyl-CoA carboxylase HFA1 Mitochondrial acetyl-coenzyme A carboxylase; catalyzes production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress; genetic and comparative analysis suggests that translation begins at a non-canonical (Ile) start codon at -372 relative to the annotated start codon lipid metabolic process, monocarboxylic acid metabolic process ligase activity mitochondrion, cytoplasm 793 BY4741 13_4 4 F 6 YMR210W - putative carboxylic ester hydrolase Putative acyltransferase with similarity to Eeb1p and Eht1p; has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification lipid metabolic process, monocarboxylic acid metabolic process transferase activity cellular_component 796 BY4741 13_4 4 F 7 YMR214W SCJ1 - One of several homologs of bacterial chaperone DnaJ; located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins response to chemical, protein folding, proteolysis involved in cellular protein catabolic process other endomembrane system, endoplasmic reticulum, cytoplasm 800 BY4741 13_4 4 F 8 YMR215W GAS3 putative 1,3-beta-glucanosyltransferase Putative 1,3-beta-glucanosyltransferase; has similarity go other GAS family members; low abundance, possibly inactive member of the GAS family of GPI-containing proteins; localizes to the cell wall; mRNA induced during sporulation biological_process transferase activity cell wall, membrane, plasma membrane 801 BY4741 13_4 4 F 9 YMR216C SKY1 serine/threonine protein kinase SKY1 SR protein kinase (SRPK); involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 response to chemical, protein targeting, RNA splicing, protein phosphorylation, regulation of transport, cellular ion homeostasis, mRNA processing, nuclear transport kinase activity, transferase activity cytoplasm 802 BY4741 13_4 4 F 10 YMR219W ESC1 - Protein localized to the nuclear periphery; involved in telomeric silencing; interacts with PAD4-domain of Sir4p chromatin organization molecular_function nucleus 805 BY4741 13_4 4 F 11 YMR221C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy biological_process molecular_function membrane, vacuole, mitochondrion, cytoplasm 807 BY4741 13_4 4 F 12 YMR222C FSH2 - Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 biological_process molecular_function cytoplasm 808 BY4741 13_4 4 G 1 YMR223W UBP8 ubiquitin-specific protease UBP8 Ubiquitin-specific protease component of the SAGA acetylation complex; required for SAGA (Spt-Ada-Gcn5-Acetyltransferase)-mediated deubiquitination of histone H2B peptidyl-amino acid modification, RNA splicing, protein alkylation, chromatin organization, histone modification, protein modification by small protein conjugation or removal peptidase activity, hydrolase activity, structural molecule activity nucleus 809 BY4741 13_4 4 G 2 YMR224C MRE11 MRX complex nuclease subunit|NGS1|XRS4|RAD58 Nuclease subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; Mre11p associates with Ser/Thr-rich ORFs in premeiotic phase; nuclease activity required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress meiotic cell cycle, DNA repair, organelle fission, sporulation, cellular response to DNA damage stimulus, DNA recombination kinase activity, transferase activity, hydrolase activity, DNA binding, structural molecule activity, nuclease activity nucleus, mitochondrion, cytoplasm 810 BY4741 13_4 4 G 3 YMR225C MRPL44 mitochondrial 54S ribosomal protein YmL44|YmL44|YMR44 Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 811 BY4741 13_4 4 G 4 YMR226C - oxidoreductase|TMA29 NADP(+)-dependent serine dehydrogenase and carbonyl reductase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments biological_process oxidoreductase activity nucleus, membrane, ribosome, cytoplasm 812 BY4741 13_4 4 G 5 YMR228W MTF1 RNA polymerase specificity factor Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation DNA-templated transcription, initiation, mitochondrion organization transcription factor activity, protein binding mitochondrial envelope, mitochondrion, cytoplasm 814 BY4741 13_4 4 G 6 YMR230W RPS10B ribosomal 40S subunit protein S10B|S10e|S10B Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication cytoplasmic translation, nucleobase-containing compound transport, nuclear transport structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 816 BY4741 13_4 4 G 7 YMR231W PEP5 tethering complex subunit PEP5|VPT11|VPS11|VPL9|VAM1|END1 Histone E3 ligase, component of CORVET membrane tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p; involved in ubiquitylation and degradation of excess histones vacuole organization, chromatin organization, regulation of transport, organelle fusion, Golgi vesicle transport, regulation of organelle organization, vesicle organization, histone modification, protein complex biogenesis, membrane fusion, endosomal transport, protein modification by small protein conjugation or removal ion binding, transferase activity, lipid binding vacuole, endomembrane system, membrane, cytoplasm 817 BY4741 13_5 4 G 8 YMR232W FUS2 - Cell fusion regulator; cytoplasmic protein localized to shmoo tip; required for alignment of parental nuclei before nuclear fusion during mating; contains a Dbl-homology domain; binds specifically with activated Cdc42p response to chemical, cell morphogenesis, organelle fusion, conjugation, nucleus organization molecular_function nucleus, mitochondrion, site of polarized growth, cytoplasm 818 BY4741 13_5 4 G 9 YMR233W TRI1 - Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication biological_process molecular_function nucleus, nucleolus, cytoplasm 819 BY4741 13_5 4 G 10 YMR234W RNH1 - Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I. RNA catabolic process nuclease activity, hydrolase activity nucleus, cytoplasm 820 BY4741 13_5 4 G 11 YMR237W BCH1 - Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication carbohydrate metabolic process, Golgi vesicle transport, cell wall organization or biogenesis molecular_function endomembrane system, ribosome, cytoplasmic membrane-bounded vesicle, Golgi apparatus, cytoplasm 823 BY4741 13_5 4 G 12 YMR238W DFG5 putative mannan endo-1,6-alpha-mannosidase Putative mannosidase; essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p cell budding, pseudohyphal growth, cell wall organization or biogenesis molecular_function endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane 824 BY4741 13_5 4 H 1 YMR241W YHM2 - Citrate and oxoglutarate carrier protein; exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome mitochondrion organization, ion transport transmembrane transporter activity, DNA binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 827 BY4741 13_5 4 H 3 YMR242C RPL20A ribosomal 60S subunit protein L20A|L20e|L20A|L18A|RPL18A2 Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 828 BY4741 13_5 4 H 4 YMR243C ZRC1 Zn(2+) transporter ZRC1|OSR1 Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication cellular ion homeostasis transmembrane transporter activity membrane, vacuole, cytoplasm 829 BY4741 13_5 4 H 5 YMR244W - - Putative protein of unknown function biological_process molecular_function cellular_component 830 BY4741 13_5 4 H 6 YMR245W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 831 BY4741 13_5 4 H 7 YMR244C-A COA6 - Protein involved in cytochrome c oxidase (Complex IV) assembly; involved in delivery of copper to Complex IV; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; ortholog implicated in cardiac defects in zebrafish and human; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress mitochondrion organization, protein complex biogenesis, cellular ion homeostasis molecular_function nucleus, mitochondrion, mitochondrial envelope, cytoplasm 832 BY4741 13_5 4 H 8 YMR246W FAA4 long-chain fatty acid-CoA ligase FAA4 Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; mutation is complemented by any of human ACSBG1, ACSL1, ACSL3, ACSL4, ACSL5, ACSL6, SLC27A2, or SLC27A4 lipid transport, cofactor metabolic process, ion transport ligase activity cytoplasm 833 BY4741 13_5 4 H 9 YMR247C RKR1 ubiquitin-protein ligase RKR1|LTN1 RING domain E3 ubiquitin ligase; involved in ubiquitin-mediated degradation of non-stop proteins and translationally stalled ER membrane proteins; component of ribosome-bound RQC (ribosome quality control) complex; degrades products of mRNAs lacking a termination codon regardless of a poly(A) tail; functional connections to chromatin modification; homolog of mouse Listerin, mutations in which reported to cause neurodegeneration chromatin organization, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process transferase activity nucleus, ribosome, cytoplasm 834 BY4741 13_5 4 H 10 YMR250W GAD1 glutamate decarboxylase GAD1 Glutamate decarboxylase; converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress response to chemical, response to oxidative stress, cellular amino acid metabolic process lyase activity cytoplasm 835 BY4741 13_5 4 H 11 YMR251W GTO3 - Omega class glutathione transferase; putative cytosolic localization other transferase activity cytoplasm 836 BY4741 13_5 4 H 12 YMR251W-A HOR7 - Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication other molecular_function membrane, endomembrane system, endoplasmic reticulum, plasma membrane, cytoplasm 837 BY4741 13_5 5 A 1 YMR252C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene biological_process molecular_function mitochondrion, cytoplasm 838 BY4741 13_5 5 A 2 YMR253C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene biological_process molecular_function membrane, cytoplasm 839 BY4741 13_5 5 A 3 YMR254C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 840 BY4741 13_5 5 A 4 YMR255W GFD1 - Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress nucleobase-containing compound transport, nuclear transport molecular_function nucleus, membrane, endomembrane system, cytoplasm 841 BY4741 13_5 5 A 6 YMR256C COX7 cytochrome c oxidase subunit VII Subunit VII of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 842 BY4741 13_5 5 A 7 YMR257C PET111 - Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other mitochondrion, membrane, mitochondrial envelope, cytoplasm 843 BY4741 13_5 5 A 8 YMR258C ROY1 - GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p protein targeting, endocytosis enzyme regulator activity nucleus, cytoplasm 844 BY4741 13_5 5 A 9 YMR259C TRM732 tRNA methylation protein TRM732 Protein involved in 2'-O-methylation of C32 of substrate tRNAs; interacts with 2'-O-ribose methyltransferase Trm7p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene; yeast null mutant can be functionally complemented by human THADA, mutations in which have been implicated in epithelial thyroid adenomas, type 2 diabetes, and polycystic ovary syndrome (PCOS) tRNA processing, RNA modification molecular_function cytoplasm 845 BY4741 13_5 5 A 10 YMR261C TPS3 - Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication carbohydrate metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups, phosphatase activity, transferase activity, hydrolase activity, enzyme regulator activity cytoplasm 847 BY4741 13_5 5 A 11 YMR262W - - Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene biological_process molecular_function cellular_component 848 BY4741 13_5 5 A 12 YNL047C SLM2 LIT1 Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; TORC2 complex substrate and effector; SLM2 has a paralog, SLM1, that arose from the whole genome duplication endosomal transport, cytoskeleton organization, signaling ion binding, lipid binding membrane, plasma membrane 7372 BY4741 00_18 5 B 1 YMR264W CUE1 KIS4 Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication response to chemical, proteolysis involved in cellular protein catabolic process enzyme regulator activity, ubiquitin-like protein binding endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm 850 BY4741 13_5 5 B 2 YMR265C - - Putative protein of unknown function biological_process molecular_function cellular_component 851 BY4741 13_5 5 B 3 YMR266W RSN1 - Membrane protein of unknown function; overexpression suppresses NaCl sensitivity of sro7 mutant cells by restoring sodium pump (Ena1p) localization to the plasma membrane Golgi vesicle transport molecular_function membrane, vacuole, cytoplasm 852 BY4741 13_5 5 B 4 YMR267W PPA2 inorganic diphosphatase PPA2|IPP2 Mitochondrial inorganic pyrophosphatase; required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate generation of precursor metabolites and energy, cellular respiration hydrolase activity mitochondrion, cytoplasm 853 BY4741 13_5 5 B 5 YMR269W TMA23 YMR268W-A Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan ribosomal small subunit biogenesis molecular_function nucleus, nucleolus, ribosome, cytoplasm 855 BY4741 13_5 5 B 6 YMR272C SCS7 fatty acid alpha-hydroxylase|FAH1 Sphingolipid alpha-hydroxylase; functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth lipid metabolic process oxidoreductase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 858 BY4741 13_5 5 B 7 YMR273C ZDS1 OSS1|NRC1|CKM1|CES1 Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication mitotic cell cycle, chromatin organization, nucleobase-containing compound transport, nuclear transport, regulation of cell cycle enzyme regulator activity cellular bud, site of polarized growth, cytoplasm 859 BY4741 13_5 5 B 8 YMR274C RCE1 CAAX prenyl protease Type II CAAX prenyl protease; involved in the proteolysis and maturation of Ras and the a-factor mating pheromone protein maturation, conjugation peptidase activity, hydrolase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 860 BY4741 13_5 5 B 9 YMR275C BUL1 ubiquitin-ubiquitin ligase BUL1|SMM2|RDS1|DAG1 Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication protein modification by small protein conjugation or removal, organelle inheritance, mitochondrion organization transferase activity membrane, plasma membrane 861 BY4741 13_5 5 B 10 YMR276W DSK2 - Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress response to chemical, cytoskeleton organization, proteolysis involved in cellular protein catabolic process, protein complex biogenesis protein binding, bridging nucleus 862 BY4741 13_5 5 B 11 YMR278W PRM15 PGM3|phosphoribomutase PRM15 Phosphoribomutase; catalyzes interconversion of ribose-1-phosphate and ribose-5-phosphate; has some phosphoglucomutase activity but primary activity in vivo is phosphoribomutase; contributes to ribose recycling in the pentose phosphate pathway; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential nucleobase-containing small molecule metabolic process isomerase activity nucleus, cytoplasm 864 BY4741 13_5 5 B 12 YMR280C CAT8 DNA-binding transcription factor CAT8|MSP8|DIL1 Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress carbohydrate metabolic process, response to chemical, transcription from RNA polymerase II promoter, monocarboxylic acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 866 BY4741 13_5 5 C 1 YMR282C AEP2 ATP13 Mitochondrial protein; likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader translational initiation, mitochondrial translation, mitochondrion organization molecular_function mitochondrion, cytoplasm 868 BY4741 13_5 5 C 2 YMR283C RIT1 tRNA A64-2'-O-ribosylphosphate transferase Initiator methionine 2'-O-ribosyl phosphate transferase; modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA tRNA processing, RNA modification transferase activity, transferring glycosyl groups, transferase activity cytoplasm 869 BY4741 13_5 5 C 3 YMR284W YKU70 ATP-dependent DNA helicase YKU70|KU70|NES24|HDF1 Subunit of the telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein to nucleus via interaction with the TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair DNA repair, telomere organization, chromatin organization, cellular response to DNA damage stimulus, DNA recombination RNA binding, DNA binding nucleus, endomembrane system, chromosome 870 BY4741 13_5 5 C 4 YMR285C NGL2 RNA exonuclease Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication rRNA processing nuclease activity, hydrolase activity other 871 BY4741 13_5 5 C 5 YMR286W MRPL33 mitochondrial 54S ribosomal protein YmL33|YmL33 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 872 BY4741 13_5 5 C 6 YMR287C DSS1 MSU1 3'-5' exoribonuclease; component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs RNA catabolic process nuclease activity, hydrolase activity mitochondrion, cytoplasm 873 BY4741 13_5 5 C 7 YMR289W ABZ2 aminodeoxychorismate lyase ABZ2 Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase); catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis cofactor metabolic process, vitamin metabolic process, cellular amino acid metabolic process lyase activity cytoplasm 875 BY4741 13_5 5 C 8 YMR291W TDA1 protein kinase TDA1 Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress protein phosphorylation kinase activity, transferase activity nucleus, cytoplasm 878 BY4741 13_5 5 C 9 YMR292W GOT1 - Homodimeric protein that is packaged into COPII vesicles; cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Golgi vesicle transport, endosomal transport molecular_function endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm 879 BY4741 13_5 5 C 10 YMR293C HER2 glutamyl-tRNA(Gln) amidotransferase subunit HER2|RRG6|GEP6|LRC6 Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 880 BY4741 13_5 5 C 11 YMR294W JNM1 PAC3|INS1 Component of the yeast dynactin complex; consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B mitotic cell cycle, cytoskeleton organization structural molecule activity cell cortex, cytoskeleton, microtubule organizing center, cytoplasm 881 BY4741 13_5 5 C 12 YMR294W-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents biological_process molecular_function cellular_component 882 BY4741 13_5 5 D 1 YMR295C - IBI2 Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; not an essential gene; protein abundance increases in response to DNA replication stress; YMR295C has a paralog, YGR273C, that arose from the whole genome duplication biological_process molecular_function cellular bud, site of polarized growth, ribosome, cytoplasm 883 BY4741 13_5 5 D 2 YMR297W PRC1 CPY1|carboxypeptidase C PRC1|CPY|LBC1 Vacuolar carboxypeptidase Y (proteinase C, CPY); broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family other peptidase activity, hydrolase activity endomembrane system, vacuole, endoplasmic reticulum, cytoplasm 885 BY4741 13_5 5 D 3 YMR299C DYN3 dynein light intermediate chain Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration other hydrolase activity cytoskeleton, cytoplasm 887 BY4741 13_5 5 D 4 YMR300C ADE4 amidophosphoribosyltransferase Phosphoribosylpyrophosphate amidotransferase (PRPPAT); catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway; also known as amidophosphoribosyltransferase nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups, transferase activity cytoplasm 888 BY4741 13_5 5 D 5 YMR302C YME2 PRP12|RNA12 Integral inner mitochondrial membrane protein; role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 890 BY4741 13_5 5 D 6 YMR303C ADH2 alcohol dehydrogenase ADH2|ADR2 Glucose-repressible alcohol dehydrogenase II; catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 cofactor metabolic process, nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process oxidoreductase activity cytoplasm 891 BY4741 13_5 5 D 7 YMR304W UBP15 ubiquitin-specific protease UBP15 Ubiquitin-specific protease involved in protein deubiquitination; forms a complex with AAA peroxins Pex1p and Pex6p; deubiquitinates mono- and polyubiquitinated forms of Pex5p; deubiquitinates Clb5p, counteracting APC activity, and facilitating both Clb5p accumulation and S phase entry; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences protein modification by small protein conjugation or removal peptidase activity, hydrolase activity peroxisome, cytoplasm 892 BY4741 13_5 5 D 8 YMR304C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 3' end of verified ORF SCW10/YMR305C biological_process molecular_function cellular_component 893 BY4741 13_5 5 D 9 YMR305C SCW10 putative family 17 glucosidase Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p; SWC10 has a paralog, SCW4, that arose from the whole genome duplication conjugation hydrolase activity, hydrolase activity, acting on glycosyl bonds cell wall, extracellular region, endomembrane system, endoplasmic reticulum, cytoplasm 894 BY4741 13_5 5 D 10 YMR306C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 3' end of ORF FKS3/YMR306W biological_process molecular_function cellular_component 896 BY4741 13_5 5 D 11 YMR307W GAS1 1,3-beta-glucanosyltransferase GAS1|GGP1|CWH52 Beta-1,3-glucanosyltransferase; required for cell wall assembly and also has a role in transcriptional silencing; localizes to cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at nuclear periphery; genetic interactions with histone H3 lysine acetyltransferases GCN5 and SAS3 indicate previously unsuspected functions for Gas1 in DNA damage response and cell cycle regulation chromatin organization, cell wall organization or biogenesis transferase activity nucleus, endomembrane system, mitochondrion, cell wall, cytoplasmic membrane-bounded vesicle, membrane, cytoplasm, plasma membrane 897 BY4741 13_5 5 D 12 YMR310C - - Putative methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; not an essential gene; YMR310C has a paralog, YGR283C, that arose from the whole genome duplication other transferase activity, methyltransferase activity nucleus 900 BY4741 13_5 5 E 1 YNL339C YRF1-6 Y' element ATP-dependent helicase protein 1 copy 6|YRF1 Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p telomere organization, DNA recombination helicase activity, hydrolase activity nucleus 1105 BY4741 14_1 5 E 2 YNL338W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is a telomeric X element core sequence on the left arm of chromosome XIV biological_process molecular_function cellular_component 1106 BY4741 14_1 5 E 3 YNL336W COS1 - Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins protein targeting, proteolysis involved in cellular protein catabolic process, endosomal transport molecular_function endomembrane system, vacuole, membrane, cytoplasm 1108 BY4741 14_1 5 E 4 YNL335W DDI3 - Cyanamide hydratase that detoxifies cyanamide; member of the HD domain metalloprotein superfamily; expression is induced over 100-fold by cyanamide and by SN2-type DNA alkylating agents such as MMS and DMA; induction decreased in rad6 and rad18 mutants; gene and protein are identical to DDI2 and Ddi2p response to chemical lyase activity cellular_component 1109 BY4741 14_1 5 E 5 YNL334C SNO2 putative pyridoxal 5'-phosphate synthase Protein of unknown function; nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin vitamin metabolic process molecular_function cellular_component 1110 BY4741 14_1 5 E 6 YNL333W SNZ2 pyridoxine biosynthesis protein SNZ2 Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p vitamin metabolic process ligase activity cellular_component 1111 BY4741 14_1 5 E 7 YNL332W THI12 - Protein involved in synthesis of the thiamine precursor HMP; member of a subtelomeric gene family including THI5, THI11, THI12, and THI13; hydroxymethylpyrimidine is also known as HMP vitamin metabolic process molecular_function cellular_component 1112 BY4741 14_1 5 E 8 YNL330C RPD3 histone deacetylase RPD3|MOF6|SDS6|SDI2|REC3 Histone deacetylase, component of both the Rpd3S and Rpd3L complexes; regulates transcription, silencing, autophagy and other processes by influencing chromatin remodeling; forms at least two different complexes which have distinct functions and members; Rpd3(L) recruitment to the subtelomeric region is regulated by interaction with the arginine methyltransferase, Hmt1p transcription from RNA polymerase I promoter, mitotic cell cycle, meiotic cell cycle, organelle fission, chromatin organization, DNA replication, DNA recombination, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation, regulation of organelle organization, histone modification, regulation of DNA metabolic process, nucleus organization, regulation of cell cycle hydrolase activity, transcription factor activity, protein binding nucleus, chromosome 1114 BY4741 14_1 5 E 9 YNL329C PEX6 AAA family ATPase peroxin 6|PAS8 AAA-peroxin; heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; mutations in human PEX6 can lead to severe peroxisomal disorders and early death protein targeting, transmembrane transport, peroxisome organization, signaling ATPase activity, hydrolase activity peroxisome, cytoplasm 1115 BY4741 14_1 5 E 10 YNL328C MDJ2 - Constituent of the mitochondrial import motor; associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain protein targeting, mitochondrion organization, transmembrane transport protein transporter activity, enzyme regulator activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1116 BY4741 14_1 5 E 11 YNL327W EGT2 - Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase; required for proper cell separation after cytokinesis; expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner other hydrolase activity, hydrolase activity, acting on glycosyl bonds cellular bud, cell wall 1117 BY4741 14_1 5 E 12 YNL326C PFA3 palmitoyltransferase PFA3 Palmitoyltransferase for Vac8p; required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions vacuole organization, protein acylation, organelle fusion, membrane fusion, protein lipidation transferase activity vacuole, membrane, cytoplasm 1118 BY4741 14_1 5 F 1 YNL324W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1119 BY4741 14_1 5 F 2 YNL325C FIG4 phosphatidylinositol-3,5-bisphosphate 5-phosphatase Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain; homologous to human FIG4, which is associated with CMT4J, a form of Charcot-Marie-Tooth disorder lipid metabolic process phosphatase activity, hydrolase activity nucleus, membrane, vacuole, cytoplasm 1120 BY4741 14_1 5 F 3 YNL323W LEM3 ROS3|BRE3 Membrane protein of the plasma membrane and ER; interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane; null mutant requires tryptophan due to mislocalization of tryptophan permease Tat2p vacuole organization, regulation of organelle organization, ion transport, lipid transport, signaling hydrolase activity, ATPase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane 1121 BY4741 14_1 5 F 4 YNL322C KRE1 - Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor cell wall organization or biogenesis structural molecule activity cell wall, membrane 1122 BY4741 14_1 5 F 5 YNL321W VNX1 - Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate ion transport transmembrane transporter activity membrane, endomembrane system, vacuole, endoplasmic reticulum, cytoplasm 1123 BY4741 14_1 5 F 6 YNL320W - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 1124 BY4741 14_1 5 F 7 YNL319W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HXT14 biological_process molecular_function cellular_component 1125 BY4741 14_1 5 F 8 YNL318C HXT14 - Protein with similarity to hexose transporter family members; expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies carbohydrate transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm 1126 BY4741 14_1 5 F 9 YNL314W DAL82 DURM Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 1130 BY4741 14_1 5 F 10 YNL311C SKP2 putative SCF ubiquitin ligase complex subunit SKP2 F-box protein of unknown function; predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments cellular amino acid metabolic process protein binding, bridging, transferase activity ribosome, cytoplasm 1133 BY4741 14_1 5 F 11 YNL309W STB1 - Protein with role in regulation of MBF-specific transcription at Start; phosphorylated by Cln-Cdc28p kinases in vitro; unphosphorylated form binds Swi6p, which is required for Stb1p function; expression is cell-cycle regulated; STB1 has a paralog, YOL131W, that arose from the whole genome duplication mitotic cell cycle, regulation of DNA metabolic process, transcription from RNA polymerase II promoter transcription factor activity, protein binding, transcription factor binding nucleus, chromosome, cytoplasm 1135 BY4741 14_1 5 F 12 YNL053W MSG5 tyrosine/serine/threonine protein phosphatase MSG5 Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p; MSG5 has a paralog, SDP1, that arose from the whole genome duplication response to chemical, protein phosphorylation, protein dephosphorylation, regulation of transport, regulation of protein modification process, conjugation, cell wall organization or biogenesis, nuclear transport, signaling phosphatase activity, hydrolase activity nucleus, cytoplasm 7373 BY4741 00_18 5 G 1 YNL305C BXI1 YBH3 Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p response to chemical, signaling molecular_function endomembrane system, vacuole, mitochondrion, membrane, endoplasmic reticulum, cytoplasm 1139 BY4741 14_1 5 G 2 YNL304W YPT11 Rab family GTPase YPT11 Rab GTPase; Myo2p-binding protein implicated in mother-to-bud transport of cortical endoplasmic reticulum (ER), late Golgi, and mitochondria during cell division; function is regulated at multiple levels; abundance of active Ypt11p forms is controlled by phosphorylation status and degradation; normally a low-abundance protein whose ER localization is only detected when protein is highly over expressed mitochondrion organization, organelle inheritance molecular_function endomembrane system, mitochondrion, cellular bud, site of polarized growth, endoplasmic reticulum, cytoplasm 1140 BY4741 14_1 5 G 3 YNL303W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1141 BY4741 14_1 5 G 4 YNL302C RPS19B ribosomal 40S subunit protein S19B|S19e|rp55B|YS16B|S19B|S16aB|RP55B Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19B has a paralog, RPS19A, that arose from the whole genome duplication cytoplasmic translation, nucleobase-containing compound transport, ribosomal small subunit biogenesis, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 1142 BY4741 14_1 5 G 5 YNL301C RPL18B ribosomal 60S subunit protein L18B|L18e|rp28B|L18B|RP28B Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 1143 BY4741 14_1 5 G 6 YNL299W TRF5 non-canonical poly(A) polymerase TRF5 Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p RNA catabolic process, snoRNA processing transferase activity, nucleotidyltransferase activity nucleus, nucleolus 1145 BY4741 14_1 5 G 7 YNL298W CLA4 serine/threonine protein kinase CLA4|ERC10 Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication vacuole organization, mitotic cell cycle, response to chemical, organelle fission, cytoskeleton organization, transmembrane transport, regulation of transport, lipid transport, transcription from RNA polymerase II promoter, protein targeting, protein phosphorylation, regulation of organelle organization, organelle inheritance, nuclear transport, regulation of cell cycle kinase activity, transferase activity nucleus, vacuole, cellular bud, cytoplasm 1146 BY4741 14_1 5 G 8 YNL086W SNN1 BLS1 Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; green fluorescent protein (GFP)-fusion protein localizes to endosomes other molecular_function endomembrane system, cytoplasm 7377 BY4741 00_18 5 G 9 YNL297C MON2 YSL2 Protein with a role in endocytosis and vacuole integrity; peripheral membrane protein; interacts with and negatively regulates Arl1p; localizes to the endosome; member of the Sec7p family of proteins protein targeting, Golgi vesicle transport, endosomal transport, endocytosis guanyl-nucleotide exchange factor activity endomembrane system, membrane, Golgi apparatus, cytoplasm 1148 BY4741 14_1 5 G 10 YNL295W MRX6 - Protein that associates with mitochondrial ribosome biological_process molecular_function cellular_component 1149 BY4741 14_1 5 G 11 YNL294C RIM21 PAL2 pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH vacuole organization, meiotic cell cycle, invasive growth in response to glucose limitation, sporulation, regulation of organelle organization, cell wall organization or biogenesis molecular_function membrane, plasma membrane 1150 BY4741 14_1 5 G 12 YNL293W MSB3 Rab GTPase-activating protein MSB3|GYP3 Rab GTPase-activating protein; regulates endocytosis via inactivation of Vps21p at endosomes and vacuole fusion via inactivation of Ypt7p at vacuoles; also acts on Ypt52p and Sec4p; localizes to plasma membrane, sites of polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; similar to TBC-domain Tre2 oncogene; MSB3 has a paralog, MSB4, that arose from the whole genome duplication; human homolog USP6NL can complement yeast msb3 msb4 double null cytoskeleton organization, exocytosis, endocytosis enzyme regulator activity cell cortex, endomembrane system, vacuole, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane 1151 BY4741 14_1 5 H 1 YNL089C - - Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins biological_process molecular_function cellular_component 7378 BY4741 00_18 5 H 3 YNL291C MID1 - N-glycosylated integral membrane protein of the ER and plasma membrane; functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer ion transport transmembrane transporter activity membrane, endomembrane system, endoplasmic reticulum, plasma membrane, cytoplasm 1153 BY4741 14_1 5 H 4 YNL289W PCL1 HCS26 Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth regulation of protein modification process, protein phosphorylation, cytoskeleton organization, regulation of cell cycle enzyme regulator activity nucleus 1155 BY4741 14_1 5 H 5 YNL288W CAF40 CCR4-NOT core subunit CAF40 Component of the CCR4-NOT transcriptional complex; evolutionarily conserved; involved in controlling mRNA initiation, elongation, and degradation; binds Cdc39p transcription from RNA polymerase II promoter, DNA-templated transcription, elongation molecular_function other 1156 BY4741 14_1 5 H 6 YNL286W CUS2 U2 snRNP complex subunit CUS2 Putative checkpoint factor in transcription; binds to U2 snRNA and Prp11p; may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) RNA splicing, mRNA processing RNA binding nucleus 1158 BY4741 14_1 5 H 7 YNL285W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1159 BY4741 14_1 5 H 8 YNL283C WSC2 STA3 Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity and recovery from heat shock; required for the arrest of secretion response; WSC2 has a paralog, WSC3, that arose from the whole genome duplication response to heat, cell wall organization or biogenesis, signaling signal transducer activity cellular bud, site of polarized growth, cytoplasm 1161 BY4741 14_1 5 H 9 YNL281W HCH1 - Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; role in regulating Hsp90 inhibitor drug sensitivity; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress response to heat, protein folding enzyme regulator activity nucleus, cytoplasm 1163 BY4741 14_1 5 H 10 YNL280C ERG24 delta(14)-sterol reductase C-14 sterol reductase; acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions but inviable on rich medium under aerobic conditions lipid metabolic process oxidoreductase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1164 BY4741 14_1 5 H 11 YNL278W CAF120 - Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication transcription from RNA polymerase II promoter molecular_function cellular_component 1166 BY4741 14_1 5 H 12 YNL096C RPS7B ribosomal 40S subunit protein S7B|S7e|rp30|S7B Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 7379 BY4741 00_18 6 A 1 YNL276C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W biological_process molecular_function cellular_component 1168 BY4741 14_1 6 A 2 YNL275W BOR1 - Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 protein targeting, ion transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm 1169 BY4741 14_1 6 A 3 YNL273W TOF1 - Subunit of a replication-pausing checkpoint complex; Tof1p-Mrc1p-Csm3p acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase; relocalizes to the cytosol in response to hypoxia mitotic cell cycle, DNA repair, organelle fission, DNA replication, cellular response to DNA damage stimulus, chromosome segregation, regulation of DNA metabolic process molecular_function nucleus, chromosome, cytoplasm 1171 BY4741 14_1 6 A 4 YNL271C BNI1 formin BNI1|SHE5|PPF3 Formin; polarisome component; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 mitotic cell cycle, cell budding, cytoskeleton organization, regulation of organelle organization, cytokinesis, protein complex biogenesis other cell cortex, cytoskeleton, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane 1173 BY4741 14_1 6 A 5 YNL270C ALP1 arginine permease ALP1|APL1 Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication ion transport, amino acid transport transmembrane transporter activity membrane, plasma membrane 1174 BY4741 14_1 6 A 7 YNL269W BSC4 - Protein of unknown function; protein-coding gene that evolved de novo via a series of point mutations in noncoding sequence; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p; may be involved in DNA repair pathway during stationary phase and contribute to robustness of cells when shifted to a nutrient-poor environment biological_process molecular_function cellular_component 1175 BY4741 14_1 6 A 8 YNL268W LYP1 lysine permease Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids ion transport, amino acid transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm 1176 BY4741 14_1 6 A 9 YNL266W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C biological_process molecular_function cellular_component 1178 BY4741 14_1 6 A 10 YNL265C IST1 - Protein with positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p endosomal transport molecular_function endomembrane system, cytoplasm 1179 BY4741 14_1 6 A 11 YNL264C PDR17 SFH4|ISS1 Phosphatidylinositol transfer protein (PITP); downregulates Plb1p-mediated turnover of phosphatidylcholine; forms a complex with Psd2p which appears essential for maintenance of vacuolar PE levels; found in the cytosol and microsomes; homologous to Pdr16p; deletion affects phospholipid composition response to chemical, ion transport, cellular amino acid metabolic process, Golgi vesicle transport, lipid metabolic process, lipid transport other endomembrane system, cytoplasm 1180 BY4741 14_1 6 A 12 YNL259C ATX1 copper metallochaperone ATX1 Cytosolic copper metallochaperone; transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake; human homolog ATOX1 can complement yeast atx1 mutant; overexpression of human ATOX1 suppresses lysine auxotrophy of the yeast sod1 null mutant, as does overexpression of yeast ATX1 response to chemical, cellular ion homeostasis, ion transport, response to oxidative stress ion binding cytoplasm 1185 BY4741 14_1 6 B 1 YNL257C SIP3 LAM3 Putative sterol transfer protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; co-localizes to puncta in the cortical ER with Ysp2p; has GRAM and StART-like (VASt) domains; one of 6 StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; previously identified as a transcription cofactor that interacts with DNA-bound Snf1p lipid transport, transcription from RNA polymerase II promoter transcription factor activity, protein binding cell cortex, endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane 1187 BY4741 14_1 6 B 2 YNL255C GIS2 mRNA-binding translational activator GIS2 Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 regulation of translation RNA binding, DNA binding, mRNA binding ribosome, cytoplasm 1189 BY4741 14_1 6 B 3 YNL254C RTC4 - Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 1190 BY4741 14_1 6 B 4 YNL253W TEX1 - Protein involved in mRNA export; component of the transcription export (TREX) complex nucleobase-containing compound transport, nuclear transport RNA binding, mRNA binding nucleus 1191 BY4741 14_1 6 B 5 YNL249C MPA43 - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 1195 BY4741 14_1 6 B 6 YNL248C RPA49 DNA-directed RNA polymerase I subunit RPA49|A49 RNA polymerase I subunit A49; essential for nucleolar assembly and for high polymerase loading rate; required for nucleolar localization of Rpa34p transcription from RNA polymerase I promoter, DNA-templated transcription, elongation transferase activity, nucleotidyltransferase activity nucleus, nucleolus 1196 BY4741 14_1 6 B 7 YNL246W VPS75 - NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia peptidyl-amino acid modification, DNA repair, protein acylation, chromatin organization, regulation of organelle organization, cellular response to DNA damage stimulus, protein complex biogenesis, histone modification, regulation of protein modification process histone binding, enzyme regulator activity nucleus, chromosome, cytoplasm 1198 BY4741 14_1 6 B 8 YOR001W RRP6 exosome nuclease subunit RRP6 Nuclear exosome exonuclease component; has 3'-5' exonuclease activity that is regulated by Lrp1p; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; role in sn/snoRNAs precursor degradation; forms a stable heterodimer with Lrp1p; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based RNA catabolic process, snoRNA processing, rRNA processing nuclease activity, hydrolase activity nucleus, nucleolus 1777 BY4741 15_1 6 B 9 YOR002W ALG6 dolichyl-P-Glc:Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase Alpha 1,3 glucosyltransferase; involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; C998T transition in human ortholog ALG6 causes carbohydrate-deficient glycoprotein syndrome type-Ic; wild-type human ortholog ALG6 can partially complement yeast alg6 mutant carbohydrate metabolic process, generation of precursor metabolites and energy, cellular respiration, protein glycosylation, lipid metabolic process transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 1778 BY4741 15_1 6 B 10 YOR003W YSP3 putative subtilisin-like protease YSP3 Putative precursor to the subtilisin-like protease III; YSP3 has a paralog, PRB1, that arose from the whole genome duplication other peptidase activity, hydrolase activity cellular_component 1779 BY4741 15_1 6 B 11 YOR005C DNL4 DNA ligase (ATP) DNL4|LIG4 DNA ligase required for nonhomologous end-joining (NHEJ); forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth; mutations in human ortholog lead to ligase IV syndrome and Dubowitz syndrome DNA repair, cellular response to DNA damage stimulus ligase activity nucleus, chromosome 1781 BY4741 15_1 6 B 12 YOR006C TSR3 - Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, cytoplasm 1782 BY4741 15_1 6 C 1 YOR007C SGT2 - Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; similar to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress response to heat, protein targeting structural molecule activity cytoplasm 1783 BY4741 15_1 6 C 2 YOR008C SLG1 WSC1|HCS77 Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response response to heat, cytoskeleton organization, regulation of transport, peroxisome organization, cell wall organization or biogenesis, signaling, response to osmotic stress, endocytosis signal transducer activity cellular bud, membrane, site of polarized growth, plasma membrane 1784 BY4741 15_1 6 C 3 YOR009W TIR4 - Cell wall mannoprotein; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins biological_process molecular_function cell wall 1785 BY4741 15_1 6 C 4 YOR010C TIR2 SRP2 Putative cell wall mannoprotein; member of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis; TIR2 has a paralog, TIR3, that arose from the whole genome duplication other molecular_function cell wall 1786 BY4741 15_1 6 C 5 YOR011W AUS1 ATP-binding cassette sterol transporter AUS1 Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen; AUS1 has a paralog, PDR11, that arose from the whole genome duplication transmembrane transport, lipid transport hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, cytoplasm, plasma membrane 1787 BY4741 15_1 6 C 6 YOR012W - - Putative protein of unknown function biological_process molecular_function cellular_component 1788 BY4741 15_1 6 C 7 YOR013W IRC11 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci DNA recombination molecular_function cellular_component 1789 BY4741 15_1 6 C 8 YOR014W RTS1 protein phosphatase 2A regulatory subunit RTS1|SCS1 B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A mitotic cell cycle, meiotic cell cycle, organelle fission, protein dephosphorylation, cytoskeleton organization, proteolysis involved in cellular protein catabolic process, regulation of organelle organization, cytokinesis, chromosome segregation, regulation of cell cycle enzyme regulator activity nucleus, cytoskeleton, chromosome, microtubule organizing center, cellular bud, site of polarized growth, cytoplasm 1790 BY4741 15_1 6 C 9 YOR015W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1791 BY4741 15_1 6 C 10 YOR016C ERP4 - Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; ERP4 has a paralog, ERP2, that arose from the whole genome duplication Golgi vesicle transport molecular_function membrane 1792 BY4741 15_1 6 C 11 YOR017W PET127 - Protein with a role in 5'-end processing of mitochondrial RNAs; located in the mitochondrial membrane mitochondrion organization molecular_function mitochondrial envelope, mitochondrion, cytoplasm 1793 BY4741 15_1 6 C 12 YOR018W ROD1 ART4 Alpha-arrestin involved in ubiquitin-dependent endocytosis; activating dephosphorylation relays glucose signaling to transporter endocytosis; calcineurin dephosphorylation is required for Rsp5p-dependent internalization of agonist-occupied Ste2p, as part of signal desensitization; recruits Rsp5p to Ste2p via its 2 PPXY motifs; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication response to chemical, regulation of transport, conjugation, endocytosis, signaling enzyme binding membrane, plasma membrane 1794 BY4741 15_1 6 D 1 YOR019W - - Protein of unknown function; may interact with ribosomes, based on co-purification experiments; YOR019W has a paralog, JIP4, that arose from the whole genome duplication mitochondrion organization molecular_function ribosome, cytoplasm 1795 BY4741 15_1 6 D 2 YOR021C SFM1 protein-arginine N-methyltransferase SFM1 SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism peptidyl-amino acid modification, protein alkylation transferase activity, methyltransferase activity cellular_component 1797 BY4741 15_1 6 D 3 YOR022C - putative carboxylic ester hydrolase Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process hydrolase activity mitochondrion, cytoplasm 1798 BY4741 15_1 6 D 4 YOR023C AHC1 - Subunit of the Ada histone acetyltransferase complex; required for structural integrity of the complex peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity nucleus 1799 BY4741 15_1 6 D 5 YOR024W - IRC12 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1800 BY4741 15_1 6 D 6 YOR025W HST3 NAD-dependent histone deacetylase HST3 Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism lipid metabolic process, chromatin organization, monocarboxylic acid metabolic process, histone modification hydrolase activity nucleus 1801 BY4741 15_1 6 D 7 YOR026W BUB3 PAC9 Kinetochore checkpoint WD40 repeat protein; localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p; functions at kinetochore to activate APC/C-Cdc20p for normal mitotic progression mitotic cell cycle, organelle fission, regulation of organelle organization, proteolysis involved in cellular protein catabolic process, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, regulation of cell cycle ubiquitin-like protein binding nucleus, chromosome 1802 BY4741 15_1 6 D 8 YOR027W STI1 Hsp90 cochaperone STI1 Hsp90 cochaperone; regulates spatial organization of amyloid-like proteins in the cytosol, thereby buffering the proteotoxicity caused by amyloid-like proteins; interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop protein folding RNA binding, mRNA binding, enzyme regulator activity cytoplasm 1803 BY4741 15_1 6 D 9 YOR028C CIN5 YAP4|HAL6 Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication response to chemical, transcription from RNA polymerase II promoter, response to osmotic stress DNA binding, transcription factor binding nucleus, cytoplasm 1804 BY4741 15_1 6 D 10 YOR029W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1805 BY4741 15_1 6 D 11 YOR030W DFG16 ZRG12|ECM41 Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p pseudohyphal growth, protein maturation molecular_function membrane, plasma membrane 1806 BY4741 15_1 6 D 12 YOR031W membrane, plasma membrane membrane, plasma membrane membrane, plasma membrane - - - 1807 BY4741 15_1 6 E 1 YOR032C HMS1 - bHLH protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant pseudohyphal growth nucleic acid binding transcription factor activity cellular_component 1808 BY4741 15_1 6 E 2 YOR033C EXO1 Rad2 family nuclease EXO1|DHS1 5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair, MMS2 error-free branch of the post replication (PRR) pathway and DNA mismatch repair; role in telomere maintenance; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress; EXO1 has a paralog, DIN7, that arose from the whole genome duplication meiotic cell cycle, DNA repair, telomere organization, cellular response to DNA damage stimulus, DNA recombination hydrolase activity, nuclease activity nucleus, cytoplasm 1809 BY4741 15_1 6 E 3 YOR034C AKR2 putative palmitoyltransferase AKR2 Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication protein acylation, protein lipidation transferase activity endomembrane system, vacuole, membrane, Golgi apparatus, cytoplasm 1810 BY4741 15_1 6 E 4 YOR035C SHE4 DIM1 Protein containing a UCS (UNC-45/CRO1/SHE4) domain; binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization cytoskeleton organization cytoskeletal protein binding cytoplasm 1811 BY4741 15_1 6 E 5 YOR036W PEP12 SNAP receptor PEP12|VPT13|VPS6|VPL6 Target membrane receptor (t-SNARE); for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin vacuole organization, protein targeting, Golgi vesicle transport, organelle inheritance other endomembrane system, Golgi apparatus, cytoplasm 1812 BY4741 15_1 6 E 6 YOR037W CYC2 - Mitochondrial peripheral inner membrane protein; contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) mitochondrion organization, protein complex biogenesis oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 1813 BY4741 15_1 6 E 7 YOR038C HIR2 SPT1 Subunit of HIR nucleosome assembly complex; involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p; relocalizes to the cytosol in response to hypoxia chromatin organization, protein complex biogenesis, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation chromatin binding, DNA binding, transcription factor activity, protein binding nucleus, cytoplasm 1814 BY4741 15_1 6 E 8 YOR039W CKB2 casein kinase 2 regulatory subunit CKB2 Beta' regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase transcription from RNA polymerase I promoter, protein phosphorylation, transcription from RNA polymerase III promoter, cellular response to DNA damage stimulus enzyme regulator activity nucleus, nucleolus 1815 BY4741 15_1 6 E 9 YOR040W GLO4 hydroxyacylglutathione hydrolase GLO4 Mitochondrial glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO4 has a paralog, GLO2, that arose from the whole genome duplication monocarboxylic acid metabolic process hydrolase activity mitochondrion, cytoplasm 1816 BY4741 15_1 6 E 10 YOR041C - SRF5 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance biological_process molecular_function cellular_component 1817 BY4741 15_1 6 E 11 YOR042W CUE5 ubiquitin-binding protein CUE5 Ubiquitin-binding protein; functions as ubiquitin-Atg8p adaptor in ubiquitin-dependent autophagy; contains CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; GFP-fusion protein localizes to cytoplasm in punctate pattern; CUE5 has a paralog, DON1, that arose from the whole genome duplication; human TOLLIP is a functional CUE-domain homolog, can complement yeast null mutant, rescuing hypersensitivity of cue5 null mutant cells to Htt-96Q proteolysis involved in cellular protein catabolic process protein binding, bridging, ubiquitin-like protein binding cytoplasm 1818 BY4741 15_1 6 E 12 YOR043W WHI2 - Protein required for full activation of the general stress response; required with binding partner Psr1p, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression response to heat, mitochondrion organization, cytoskeleton organization, response to osmotic stress, endocytosis enzyme regulator activity cellular_component 1819 BY4741 15_1 6 F 1 YOR044W IRC23 - Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm 1820 BY4741 15_1 6 F 2 YOR045W TOM6 MOM8B|ISP6 Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex protein targeting, mitochondrion organization, transmembrane transport, protein complex biogenesis protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1821 BY4741 15_1 6 F 3 YOR047C STD1 SFS3|MSN3 Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+]; STD1 has a paralog, MTH1, that arose from the whole genome duplication carbohydrate metabolic process, nucleobase-containing small molecule metabolic process, transcription from RNA polymerase II promoter, response to osmotic stress, signaling enzyme regulator activity nucleus, membrane, plasma membrane 1823 BY4741 15_1 6 F 4 YOR049C RSB1 phospholipid-translocating ATPase RSB1 Suppressor of sphingoid LCB sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport long chain bases (LCBs) from the cytoplasmic side toward the extracytoplasmic side of the membrane ion transport, lipid transport hydrolase activity, ATPase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane 1825 BY4741 15_1 6 F 5 YOR050C - YOR29-01 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant is viable biological_process molecular_function cellular_component 1826 BY4741 15_1 6 F 6 YOR051C ETT1 NRO1|YOR29-02 Nuclear protein that inhibits replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough other molecular_function nucleus 1827 BY4741 15_1 6 F 7 YOR052C TMC1 YOR29-03 AN1-type zinc finger protein of unknown function; may protect cells from trivalent metalloid induced proteotoxicity; contains a PACE promoter element, a transcriptional profile similar to CUZ1 and RPN2, and decreased expression in an RPN4 mutant; induced by nitrogen limitation and weak acid; ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress response to chemical molecular_function nucleus 1828 BY4741 15_1 6 F 8 YOR053W - YOR29-04 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C biological_process molecular_function cellular_component 1829 BY4741 15_1 6 F 9 YOR054C VHS3 phosphopantothenoylcysteine decarboxylase complex subunit VHS3|YOR29-05 Negative regulatory subunit of protein phosphatase 1 Ppz1p; involved in coenzyme A biosynthesis; subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex and the CoA-Synthesizing Protein Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p) cofactor metabolic process, cellular ion homeostasis, nucleobase-containing small molecule metabolic process lyase activity, enzyme regulator activity cytoplasm 1830 BY4741 15_1 6 F 10 YOR055W - YOR29-06 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1831 BY4741 15_1 6 F 11 YOR058C ASE1 YOR29-09 Mitotic spindle midzone-localized microtubule bundling protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress mitotic cell cycle, cytoskeleton organization, regulation of organelle organization, organelle assembly, protein complex biogenesis, regulation of cell cycle cytoskeletal protein binding nucleus, cytoskeleton, cytoplasm 1834 BY4741 15_1 6 F 12 YOR059C LPL1 putative hydrolase|YOR29-10 Phospholipase; contains lipase specific GXSXG motif; maintains lipid droplet (LD) morphology; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress other hydrolase activity other 1835 BY4741 15_1 6 G 1 YOR061W CKA2 casein kinase 2 catalytic subunit CKA2|YOR29-12 Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching transcription from RNA polymerase I promoter, protein phosphorylation, transcription from RNA polymerase III promoter, cellular response to DNA damage stimulus, response to osmotic stress kinase activity, transferase activity nucleus, nucleolus 1837 BY4741 15_1 6 G 2 YOR062C - YOR29-13 Protein of unknown function; similar to Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; YOR062C has a paralog, YKR075C, that arose from the whole genome duplication biological_process molecular_function nucleus, cytoplasm 1838 BY4741 15_1 6 G 3 YOR064C YNG1 YOR29-15 Subunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3; shares significant sequence identity with the human candidate tumor suppressor p33-ING1 in C-terminal region peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation transferase activity, histone binding nucleus 1840 BY4741 15_1 6 G 4 YOR065W CYT1 ubiquinol--cytochrome-c reductase catalytic subunit CYT1|YOR29-16|CTC1 Cytochrome c1; component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process other mitochondrion, membrane, mitochondrial envelope, cytoplasm 1841 BY4741 15_1 6 G 5 YOR066W MSA1 YOR29-17 Activator of G1-specific transcription factors MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; MSA1 has a paralog, MSA2, that arose from the whole genome duplication mitotic cell cycle, transcription from RNA polymerase II promoter molecular_function nucleus, cytoplasm 1842 BY4741 15_1 6 G 6 YOR067C ALG8 dolichyl-P-Glc:Glc1Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase|YOR29-18 Glucosyl transferase; involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p; human homolog ALG8 can complement yeast null mutant carbohydrate metabolic process, protein glycosylation, lipid metabolic process transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 1843 BY4741 15_1 6 G 7 YOR068C VAM10 YOR29-19 Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering vacuole organization, membrane fusion, organelle fusion molecular_function membrane, vacuole, cytoplasm 1844 BY4741 15_1 6 G 8 YOR069W VPS5 sorting nexin 1|YOR29-20|VPT5|PEP10|GRD2 Nexin-1 homolog; required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to late Golgi; structural component of retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes; VPS5 has a paralog, YKR078W, that arose from the whole genome duplication endosomal transport protein transporter activity, ion binding, lipid binding membrane, endomembrane system, cytoplasm 1845 BY4741 15_1 6 G 9 YOR070C GYP1 YOR29-21 Cis-golgi GTPase-activating protein (GAP) for yeast Rabs; the Rab family members are Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion other enzyme regulator activity endomembrane system, mitochondrion, cytoplasm, Golgi apparatus 1846 BY4741 15_1 6 G 10 YOR071C NRT1 THI71|YOR29-22 High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; major transporter for 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine) uptake; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, plasma membrane 1847 BY4741 15_1 6 G 11 YOR072W - YOR29-23 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive biological_process molecular_function cellular_component 1848 BY4741 15_1 6 G 12 YOR073W SGO1 YOR29-24 Component of the spindle checkpoint; involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability; recruits condensin to the pericentric region of chromosomes during meiosis; dissociates from pericentromeres when sister kinetochores are under tension mitotic cell cycle, meiotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, proteolysis involved in cellular protein catabolic process, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, regulation of cell cycle molecular_function nucleus, cytoskeleton, microtubule organizing center, chromosome 1849 BY4741 15_1 6 H 1 YOR076C SKI7 YOR29-27 GTP-binding protein that couples the Ski complex and exosome; putative pseudo-translational GTPase involved in 3'-to-5' mRNA decay pathway; interacts with both the cytoplasmic exosome and the Ski complex; eRF3-like domain targets nonstop mRNA for degradation; null mutants have a superkiller phenotype; SKI7 has a paralog, HBS1, that arose from the whole genome duplication RNA catabolic process protein binding, bridging, ion binding cytoplasm 1852 BY4741 15_1 6 H 3 YOR078W BUD21 YOR29-29|UTP16 Component of small ribosomal subunit (SSU) processosome; this complex contains U3 snoRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; originally isolated as bud-site selection mutant that displays a random budding pattern rRNA processing, ribosomal small subunit biogenesis RNA binding nucleus, nucleolus 1854 BY4741 15_1 6 H 4 YOR079C ATX2 Mn(2+) transporter ATX2|YOR29-30 Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation cellular ion homeostasis transmembrane transporter activity membrane, endomembrane system, cytoplasm, Golgi apparatus 1855 BY4741 15_1 6 H 5 YOR080W DIA2 DNA-binding SCF ubiquitin ligase subunit DIA2|YOR29-31 Origin-binding F-box protein; forms SCF ubiquitin ligase complex with Skp1p and Cdc53p; functions in ubiquitylation of silent chromatin structural protein Sir4p; required to target Cdc6p for destruction during G1 phase; required for deactivation of Rad53 checkpoint kinase, completion of DNA replication during recovery from DNA damage, assembly of RSC complex, RSC-mediated transcription regulation, and nucleosome positioning; involved in invasive and pseudohyphal growth invasive growth in response to glucose limitation, chromatin organization, DNA replication, proteolysis involved in cellular protein catabolic process, protein modification by small protein conjugation or removal, regulation of DNA metabolic process transferase activity, DNA binding nucleus, chromosome 1856 BY4741 15_1 6 H 6 YOR081C TGL5 STC2 Bifunctional triacylglycerol lipase and LPA acyltransferase; lipid particle-localized triacylglycerol (TAG) lipase involved in triacylglycerol mobilization; catalyzes acylation of lysophosphatidic acid (LPA); potential Cdc28p substrate; TGL5 has a paralog, TGL4, that arose from the whole genome duplication lipid metabolic process transferase activity, hydrolase activity other 1857 BY4741 15_1 6 H 7 YOR082C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR083W biological_process molecular_function membrane 1858 BY4741 15_1 6 H 8 YOR083W WHI5 transcriptional repressor WHI5 Repressor of G1 transcription; binds to SCB binding factor (SBF) at SCB target promoters in early G1; dilution of Whi5p concentration during cell growth determines cell size; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1 mitotic cell cycle, transcription from RNA polymerase II promoter transcription factor activity, protein binding nucleus, cytoplasm 1859 BY4741 15_1 6 H 9 YOR084W LPX1 - Peroxisomal matrix-localized lipase; required for normal peroxisome morphology; contains a peroxisomal targeting signal type 1 (PTS1) and a lipase motif; peroxisomal import requires the PTS1 receptor, Pex5p and self-interaction; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; oleic acid inducible lipid metabolic process, peroxisome organization hydrolase activity peroxisome, cytoplasm 1860 BY4741 15_1 6 H 10 YOR085W OST3 dolichyl-diphosphooligosaccharide--protein glycotransferase OST3 Gamma subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins carbohydrate metabolic process, protein complex biogenesis, protein glycosylation transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 1861 BY4741 15_1 6 H 11 YOR086C TCB1 - Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in PM; contains 3 calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact; TCB1 has a paralog, TCB2, that arose from the whole genome duplication lipid metabolic process lipid binding cell cortex, endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm, plasma membrane 1862 BY4741 15_1 6 H 12 YOR087W YVC1 TRPY1|YOR088W Vacuolar cation channel; mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock cellular ion homeostasis transmembrane transporter activity membrane, vacuole, cytoplasm 1863 BY4741 15_1 7 A 1 YOR088W membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - 1864 BY4741 15_1 7 A 2 YOR089C VPS21 Rab family GTPase VPS21|YPT51|YPT21|VPT12|VPS12 Endosomal Rab family GTPase; required for endocytic transport and sorting of vacuolar hydrolases; required for endosomal localization of the CORVET complex; required with YPT52 for MVB biogenesis and sorting; involved in autophagy and ionic stress tolerance; geranylgeranylation required for membrane association; protein abundance increases in response to DNA replication stress; mammalian Rab5 homolog; VPS21 has a paralog, YPT53, that arose from the whole genome duplication vacuole organization, protein targeting, organelle assembly, Golgi vesicle transport, organelle inheritance, endosomal transport, endocytosis GTPase activity, hydrolase activity endomembrane system, mitochondrion, membrane, mitochondrial envelope, cytoplasm 1865 BY4741 15_1 7 A 3 YOR090C PTC5 type 2C protein phosphatase PTC5|PPP1 Mitochondrial type 2C protein phosphatase (PP2C); involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p protein dephosphorylation phosphatase activity, hydrolase activity mitochondrial envelope, mitochondrion, cytoplasm 1866 BY4741 15_1 7 A 4 YOR091W TMA46 RBF46 Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p cytoplasmic translation RNA binding, mRNA binding ribosome, cytoplasm 1867 BY4741 15_1 7 A 5 YOR092W ECM3 putative ATPase ECM3|YOR3165W Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication biological_process ATPase activity, hydrolase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1868 BY4741 15_1 7 A 6 YOR093C - - Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents biological_process molecular_function membrane 1869 BY4741 15_1 7 A 8 YOR094W ARF3 Arf family GTPase ARF3|ARL2 Glucose-repressible ADP-ribosylation factor; GTPase of Ras superfamily involved in regulating cell polarity and invasive growth; localizes to dynamic spots at plasma membrane and modulates PtdIns(4,5)P2 levels to facilitate endocytosis; required for localization of endocytic protein Lsb5p to correct cortical site in cells; also has mRNA binding activity; homolog of mammalian Arf6 mitotic cell cycle, cytoskeleton organization, cytokinesis, lipid metabolic process, endocytosis RNA binding, GTPase activity, hydrolase activity, enzyme regulator activity, mRNA binding cellular bud, site of polarized growth 1870 BY4741 15_1 7 A 9 YOR289W - - Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 1585 BY4741 15_4 7 A 10 YOR290C SNF2 SWI/SNF catalytic subunit SNF2|TYE3|SWI2|HAF1|GAM1 Catalytic subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p chromatin organization, DNA replication, DNA recombination, transcription from RNA polymerase II promoter, DNA repair, carbohydrate metabolic process, invasive growth in response to glucose limitation, cellular response to DNA damage stimulus, response to starvation, regulation of DNA metabolic process, oligosaccharide metabolic process chromatin binding, hydrolase activity, histone binding, DNA binding, ATPase activity, transcription factor binding nucleus 1586 BY4741 15_4 7 A 11 YOR291W YPK9 - Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome biological_process molecular_function membrane, vacuole, cytoplasm 1587 BY4741 15_4 7 A 12 YOR292C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene biological_process molecular_function membrane, vacuole, cytoplasm 1588 BY4741 15_4 7 B 1 YOR293W RPS10A ribosomal 40S subunit protein S10A|S10e|S10A Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication cytoplasmic translation, nucleobase-containing compound transport, nuclear transport structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 1589 BY4741 15_4 7 B 2 YOR295W UAF30 - Subunit of UAF (upstream activation factor) complex; UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; targeting factor for the UAF that facilitates activation of many rDNA genes; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication transcription from RNA polymerase I promoter, chromatin organization nucleic acid binding transcription factor activity, DNA binding nucleus, nucleolus 1591 BY4741 15_4 7 B 3 YOR296W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene biological_process molecular_function cytoplasm 1592 BY4741 15_4 7 B 4 YOR297C TIM18 - Component of the mitochondrial TIM22 complex; involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex response to chemical, protein targeting, mitochondrion organization, transmembrane transport, response to oxidative stress, response to osmotic stress protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1593 BY4741 15_4 7 B 5 YOR298W MUM3 - Protein of unknown function involved in outer spore wall organization; has similarity to the tafazzins superfamily of acyltransferases meiotic cell cycle, lipid metabolic process, sporulation, cell wall organization or biogenesis transferase activity cellular_component 1594 BY4741 15_4 7 B 6 YOR299W BUD7 - Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication mitotic cell cycle, carbohydrate metabolic process, cytokinesis, Golgi vesicle transport, cell wall organization or biogenesis molecular_function endomembrane system, cytoplasmic membrane-bounded vesicle, Golgi apparatus, cytoplasm 1595 BY4741 15_4 7 B 7 YOR301W RAX1 - Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily mitotic cell cycle, cytokinesis molecular_function cellular bud, membrane, site of polarized growth 1597 BY4741 15_4 7 B 8 YOR302W - - CPA1 uORF; Arginine attenuator peptide, regulates translation of the CPA1 mRNA regulation of translation other cytoplasm 1598 BY4741 15_4 7 B 9 YOR303W CPA1 carbamoyl-phosphate synthase (glutamine-hydrolyzing) CPA1 Small subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader cellular amino acid metabolic process ligase activity cytoplasm 1599 BY4741 15_4 7 B 10 YOR304C-A BIL1 EDO1 Protein that binds Bud6p and has a role in actin cable assembly; involved in the Bnr1p-dependent pathway of cable assembly; localizes to bud tip and bud neck cytoskeleton organization molecular_function cellular bud, site of polarized growth, cytoplasm 1600 BY4741 15_4 7 B 11 YOR304W ISW2 - ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth; the Isw2 complex exhibits basal levels of chromatin binding throughout the genome as well as target-specific chromatin interactions; targeted by Ume6p- and Sua7p-dependent DNA looping to many loci genome-wide response to chemical, chromatin organization, DNA-templated transcription, termination, transcription from RNA polymerase II promoter chromatin binding, hydrolase activity, DNA binding, ATPase activity nucleus 1601 BY4741 15_4 7 B 12 YOR305W RRG7 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene biological_process molecular_function mitochondrion, cytoplasm 1602 BY4741 15_4 7 C 1 YOR307C SLY41 - Protein involved in ER-to-Golgi transport Golgi vesicle transport molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1604 BY4741 15_4 7 C 2 YOR308C SNU66 U4/U6-U5 snRNP complex subunit SNU66 Component of the U4/U6.U5 snRNP complex; involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 RNA splicing, rRNA processing, mRNA processing molecular_function nucleus 1605 BY4741 15_4 7 C 3 YOR311C DGK1 diacylglycerol kinase|HSD1 Diacylglycerol kinase; localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain lipid metabolic process kinase activity, transferase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1608 BY4741 15_4 7 C 4 YOR312C RPL20B ribosomal 60S subunit protein L20B|L20e|L20B|L18B|RPL18A1 Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 1609 BY4741 15_4 7 C 5 YOR313C SPS4 - Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage meiotic cell cycle, sporulation molecular_function other 1610 BY4741 15_4 7 C 6 YOR314W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1611 BY4741 15_4 7 C 7 YOR315W SFG1 - Nuclear protein putative transcription factor; required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate mitotic cell cycle, pseudohyphal growth molecular_function nucleus, cytoplasm 1612 BY4741 15_4 7 C 8 YOR316C COT1 metal cation transporter COT1 Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication cellular ion homeostasis, ion transport transmembrane transporter activity membrane, vacuole, mitochondrion, cytoplasm 1613 BY4741 15_4 7 C 9 YOR318C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo biological_process molecular_function cellular_component 1615 BY4741 15_4 7 C 10 YOR320C GNT1 glucose N-acetyltransferase N-acetylglucosaminyltransferase; capable of modification of N-linked glycans in the Golgi apparatus protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups, transferase activity endomembrane system, cytoplasm, Golgi apparatus 1617 BY4741 15_4 7 C 11 YOR321W PMT3 dolichyl-phosphate-mannose-protein mannosyltransferase PMT3 Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication carbohydrate metabolic process, protein glycosylation transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 1618 BY4741 15_4 7 C 12 YOR322C LDB19 ART1 Alpha-arrestin involved in ubiquitin-dependent endocytosis; regulates endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its targets; involved in the basal internalization and turnover of alpha-factor receptor Ste2p; recruits ubiquitin ligase Rsp5p to Ste2p via its 2 PPXY motifs; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane regulation of transport, endocytosis enzyme binding endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, Golgi apparatus, cytoplasm, plasma membrane 1619 BY4741 15_4 7 D 1 YOR323C PRO2 glutamate-5-semialdehyde dehydrogenase Gamma-glutamyl phosphate reductase; catalyzes the second step in proline biosynthesis cellular amino acid metabolic process oxidoreductase activity nucleus, cytoplasm 1620 BY4741 15_4 7 D 2 YOR324C FRT1 HPH1 Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication response to osmotic stress molecular_function endomembrane system, endoplasmic reticulum, cytoplasm 1621 BY4741 15_4 7 D 3 YOR327C SNC2 SNAP receptor SNC2 Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; Snc2p levels regulated by Vps45p; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication organelle fusion, vesicle organization, membrane fusion, Golgi vesicle transport, exocytosis, endocytosis other endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, Golgi apparatus, cytoplasm 1624 BY4741 15_4 7 D 4 YOR328W PDR10 ATP-binding cassette multidrug transporter PDR10 ATP-binding cassette (ABC) transporter; multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p response to chemical, transmembrane transport ATPase activity, hydrolase activity, transmembrane transporter activity membrane, plasma membrane 1625 BY4741 15_4 7 D 5 YOR330C MIP1 DNA-directed DNA polymerase gamma MIP1 Mitochondrial DNA polymerase gamma; single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoan complex of catalytic and accessory subunits; polymorphic in yeast, petites occur more frequently in some lab strains; human ortholog POLG complements yeast mip1 mutant; mutations in human POLG associated with Alpers-Huttenlocher syndrome (AHS), progressive external ophthalmoplegia (PEO), parkinsonism, other mitochondrial diseases DNA replication, mitochondrion organization transferase activity, nucleotidyltransferase activity, hydrolase activity, nuclease activity mitochondrion, cytoplasm 1627 BY4741 15_4 7 D 6 YOR332W VMA4 H(+)-transporting V1 sector ATPase subunit E Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress cellular ion homeostasis ATPase activity, hydrolase activity, transmembrane transporter activity membrane, vacuole, cytoplasm 1629 BY4741 15_4 7 D 7 YOR334W MRS2 - Mitochondrial inner membrane Mg(2+) channel; required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns; similar to bacterial CorA ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 1631 BY4741 15_4 7 D 8 YOR337W TEA1 - Ty1 enhancer activator involved in Ty enhancer-mediated transcription; required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein other DNA binding nucleus, cell cortex, cytoplasm 1634 BY4741 15_4 7 D 9 YOR338W - - Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication meiotic cell cycle, sporulation, transcription from RNA polymerase II promoter molecular_function cellular_component 1635 BY4741 15_4 7 D 10 YOR339C UBC11 putative E2 ubiquitin-protein ligase UBC11 Ubiquitin-conjugating enzyme; most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 protein modification by small protein conjugation or removal transferase activity cytoplasm 1636 BY4741 15_4 7 D 11 YOR342C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication biological_process molecular_function nucleus, cytoplasm 1639 BY4741 15_4 7 D 12 YOR343C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1640 BY4741 15_4 7 E 1 YOR344C TYE7 SGC1 Serine-rich protein that contains a bHLH DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression; bHLH stands for basic-helix-loop-helix carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus, chromosome 1641 BY4741 15_4 7 E 2 YOR346W REV1 - Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress DNA repair, cellular response to DNA damage stimulus transferase activity, nucleotidyltransferase activity nucleus, chromosome, mitochondrion, cytoplasm 1643 BY4741 15_4 7 E 3 YOR347C PYK2 pyruvate kinase PYK2 Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication monocarboxylic acid metabolic process transferase activity mitochondrion, cytoplasm 1644 BY4741 15_4 7 E 4 YOR348C PUT4 proline permease PUT4 Proline permease; required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells ion transport, amino acid transport transmembrane transporter activity membrane, plasma membrane 1645 BY4741 15_4 7 E 5 YOR349W CIN1 - Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl protein folding, protein complex biogenesis cytoskeletal protein binding cellular_component 1646 BY4741 15_4 7 E 6 YOR350C MNE1 - Protein involved in splicing Group I aI5-beta intron from COX1 mRNA; mitochondrial matrix protein RNA splicing molecular_function mitochondrion, cytoplasm 1647 BY4741 15_4 7 E 7 YOR351C MEK1 serine/threonine protein kinase MEK1|MRE4 Meiosis-specific serine/threonine protein kinase; functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids; stabilizes Hop1-Thr318 phosphorylation to promote interhomolog recombination and checkpoint responses during meiosis meiotic cell cycle, protein phosphorylation, organelle fission, regulation of organelle organization, regulation of cell cycle kinase activity, transferase activity nucleus 1648 BY4741 15_4 7 E 8 YOR352W TFB6 TFIIH complex subunit TFB6 Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus, cytoplasm 1649 BY4741 15_4 7 E 9 YOR354C MSC6 - Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies meiotic cell cycle, organelle fission, DNA recombination RNA binding, mRNA binding mitochondrion, cytoplasm 1651 BY4741 15_4 7 E 10 YOR355W GDS1 - Protein of unknown function; required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies generation of precursor metabolites and energy, cellular respiration molecular_function nucleus, mitochondrion, cytoplasm 1652 BY4741 15_4 7 E 11 YOR356W CIR2 - Putative ortholog of human ETF-dH; found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response; ETF-dH is also known as electron transfer flavoprotein dehydrogenase biological_process molecular_function mitochondrion, cytoplasm 1653 BY4741 15_4 7 E 12 YOR357C SNX3 GRD19 Sorting nexin for late-Golgi enzymes; required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Golgi vesicle transport, endosomal transport ion binding, lipid binding endomembrane system, cytoplasm 1654 BY4741 15_4 7 F 1 YOR358W HAP5 - Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex response to chemical, transcription from RNA polymerase II promoter, generation of precursor metabolites and energy, cellular respiration nucleic acid binding transcription factor activity, DNA binding nucleus 1655 BY4741 15_4 7 F 2 YOR359W VTS1 - Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity RNA catabolic process, regulation of DNA metabolic process RNA binding, DNA binding nucleus, cytoplasm 1656 BY4741 15_4 7 F 3 YOR360C PDE2 3',5'-cyclic-nucleotide phosphodiesterase PDE2|SRA5 High-affinity cyclic AMP phosphodiesterase; component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon signaling hydrolase activity nucleus, cytoplasm 1657 BY4741 15_4 7 F 4 YOR363C PIP2 oleate-activated transcription factor PIP2|OAF2 Autoregulatory, oleate-activated transcription factor; subunit of a heterodimeric complex with Oaf1p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; PIP2 has a paralog, OAF1, that arose from the whole genome duplication response to chemical, lipid metabolic process, transcription from RNA polymerase II promoter, monocarboxylic acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus, membrane 1660 BY4741 15_4 7 F 5 YOR365C - putative FAD transporter Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication biological_process molecular_function membrane 1662 BY4741 15_4 7 F 6 YOR367W SCP1 - Component of yeast cortical actin cytoskeleton; binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin cytoskeleton organization protein binding, bridging, cytoskeletal protein binding cell cortex, cytoskeleton, cytoplasm 1664 BY4741 15_4 7 F 7 YOR368W RAD17 - Checkpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins meiotic cell cycle, DNA repair, organelle fission, DNA recombination, cellular response to DNA damage stimulus DNA binding nucleus, chromosome 1665 BY4741 15_4 7 F 8 YOR371C GPB1 KRH2 Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication response to chemical, invasive growth in response to glucose limitation, proteolysis involved in cellular protein catabolic process, protein modification by small protein conjugation or removal, pseudohyphal growth, signaling enzyme regulator activity, signal transducer activity membrane, cytoplasm, plasma membrane 1668 BY4741 15_4 7 F 9 YOR374W ALD4 aldehyde dehydrogenase (NADP(+)) ALD4|ALDH2|ALD7 Mitochondrial aldehyde dehydrogenase; required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed; can substitute for cytosolic NADP-dependent aldehyde dehydrogenase when directed to the cytosol; human homolog ALDH2 can complement yeast ald4 mutant cofactor metabolic process, nucleobase-containing small molecule metabolic process, monocarboxylic acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 1671 BY4741 15_4 7 F 10 YOR375C GDH1 glutamate dehydrogenase (NADP(+)) GDH1|DHE4|GDHA|GDH-A|URE1 NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication cellular amino acid metabolic process oxidoreductase activity nucleus, cytoplasm 1672 BY4741 15_4 7 F 11 YOR376W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene biological_process molecular_function membrane 1673 BY4741 15_4 7 F 12 YOR377W ATF1 alcohol O-acetyltransferase Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism generation of precursor metabolites and energy transferase activity other 1674 BY4741 15_4 7 G 1 YOR378W AMF1 - Low affinity NH4+ transporter; member of the DHA2 family of drug:H+ anti porters; putative paralog of ATR1; but not required for boron tolerance; non-essential gene ion transport transmembrane transporter activity membrane, plasma membrane 1675 BY4741 15_4 7 G 2 YOR380W RDR1 - Transcriptional repressor involved in regulating multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins response to chemical nucleic acid binding transcription factor activity, DNA binding nucleus 1677 BY4741 15_4 7 G 3 YOR381W FRE3 ferric-chelate reductase Ferric reductase; reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels cellular ion homeostasis oxidoreductase activity membrane, plasma membrane 1678 BY4741 15_4 7 G 4 YOR382W FIT2 - Mannoprotein that is incorporated into the cell wall; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell wall other molecular_function cell wall 1679 BY4741 15_4 7 G 5 YOR383C FIT3 - Mannoprotein that is incorporated into the cell wall; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell wall other molecular_function cell wall 1680 BY4741 15_4 7 G 6 YOR384W FRE5 putative ferric-chelate reductase Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process oxidoreductase activity membrane, mitochondrion, cytoplasm 1681 BY4741 15_5 7 G 7 YOR385W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene biological_process molecular_function cytoplasm 1682 BY4741 15_5 7 G 8 YOR386W PHR1 deoxyribodipyrimidine photo-lyase PHR1 DNA photolyase involved in photoreactivation; repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p DNA repair, cellular response to DNA damage stimulus RNA binding, lyase activity, mRNA binding nucleus, mitochondrion, cytoplasm 1683 BY4741 15_5 7 G 9 YOL001W PHO80 phoR|VAC5|TUP7|AGS3 Cyclin; interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling protein phosphorylation, cellular ion homeostasis, regulation of protein modification process, transcription from RNA polymerase II promoter, regulation of cell cycle, signaling enzyme regulator activity nucleus 1692 BY4741 15_5 7 G 10 YOL002C IZH2 PHO36 Plasma membrane receptor for plant antifungal osmotin; involved in zinc ion homeostasis, apoptosis; negatively regulates ZRT1 and other functionally divergent genes through CCCTC promoter motif (IzRE); modulates FET3 activity in iron-independent manner; affects gene expression by influencing balance of competition between Msn2p/Msn4p and Nrg1p/Nrg2p for binding to IzRE; transcription regulated by Zap1p, zinc, fatty acid levels; homolog of mammalian adiponectin receptor response to chemical, cellular ion homeostasis, transcription from RNA polymerase II promoter other membrane, plasma membrane 1693 BY4741 15_5 7 G 11 YOL003C PFA4 palmitoyltransferase PFA4 Palmitoyltransferase with autoacylation activity; required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases protein acylation, protein lipidation transferase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1694 BY4741 15_5 7 G 12 YOL004W SIN3 transcriptional regulator SIN3|UME4|SDS16|SDI1|RPD1|GAM2|CPE1 Component of both the Rpd3S and Rpd3L histone deacetylase complexes; involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity transcription from RNA polymerase I promoter, response to heat, mitotic cell cycle, meiotic cell cycle, DNA repair, chromatin organization, DNA replication, regulation of organelle organization, cellular response to DNA damage stimulus, histone modification, regulation of DNA metabolic process, transcription from RNA polymerase II promoter transcription factor activity, protein binding nucleus, chromosome 1695 BY4741 15_5 7 H 1 YOL006C TOP1 DNA topoisomerase 1|MAK17|MAK1 Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks; enzymatic activity and interaction with Nsr1p are negatively regulated by polyphosphorylation mitotic cell cycle, organelle fission, chromatin organization, DNA replication, chromosome segregation, DNA recombination, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation isomerase activity nucleus, chromosome, nucleolus 1697 BY4741 15_5 7 H 3 YOL007C CSI2 - Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene biological_process molecular_function cellular bud, site of polarized growth, vacuole, cytoplasm 1698 BY4741 15_5 7 H 4 YOL008W COQ10 ubiquinone-binding protein COQ10 Coenzyme Q (ubiquinone) binding protein; functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes; respiratory growth defect of the null mutant is functionally complemented by human COQ10A cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration other mitochondrion, membrane, mitochondrial envelope, cytoplasm 1699 BY4741 15_5 7 H 5 YOL009C MDM12 ERMES complex subunit MDM12 Mitochondrial outer membrane protein, ERMES complex subunit; required for transmission of mitochondria to daughter cells; required for mitophagy; may influence import and assembly of outer membrane beta-barrel proteins; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase mitochondrion organization, ion transport, protein complex biogenesis, lipid transport, organelle inheritance molecular_function nucleus, endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm, plasma membrane 1700 BY4741 15_5 7 H 6 YOL011W PLB3 - Phospholipase B (lysophospholipase) involved in lipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro; PLB3 has a paralog, PLB1, that arose from the whole genome duplication lipid metabolic process, cellular amino acid metabolic process hydrolase activity membrane, extracellular region, plasma membrane 1702 BY4741 15_5 7 H 7 YOL012C HTZ1 histone H2AZ|H2AZ|H2A.F/Z|HTA3 Histone variant H2AZ; exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin; Htz1p-containing nucleosomes facilitate RNA Pol II passage by affecting correct assembly and modification status of RNA Pol II elongation complexes and by favoring efficient nucleosome remodeling RNA catabolic process, chromatin organization, transcription from RNA polymerase II promoter chromatin binding nucleus, chromosome 1703 BY4741 15_5 7 H 8 YOL013C HRD1 E3 ubiquitin-protein ligase HRD1|DER3 Ubiquitin-protein ligase; functions in ER retention of misfolded proteins; required for ER-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon response to chemical, proteolysis involved in cellular protein catabolic process, cell wall organization or biogenesis, signaling transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 1704 BY4741 15_5 7 H 9 YOL014W - - Putative protein of unknown function biological_process molecular_function cellular_component 1705 BY4741 15_5 7 H 10 YOL015W IRC10 - Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function membrane 1706 BY4741 15_5 7 H 11 YOL017W ESC8 - Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication chromatin organization molecular_function nucleus 1708 BY4741 15_5 7 H 12 YOL018C TLG2 - Syntaxin-like t-SNARE; forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) protein targeting, organelle fusion, vesicle organization, membrane fusion, Golgi vesicle transport, lipid metabolic process, endocytosis other endomembrane system, membrane, Golgi apparatus, cytoplasm 1709 BY4741 15_5 8 A 1 YOL019W - TOS7 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication biological_process molecular_function membrane, vacuole, plasma membrane, cytoplasm 1710 BY4741 15_5 8 A 2 YOL020W TAT2 aromatic amino acid transmembrane transporter TAT2|TAP2|SCM2|SAB2|LTG3 High affinity tryptophan and tyrosine permease; overexpression confers FK506 and FTY720 resistance ion transport, amino acid transport transmembrane transporter activity membrane, plasma membrane 1711 BY4741 15_5 8 A 3 YOL023W IFM1 translation initiation factor 2 Mitochondrial translation initiation factor 2 translational initiation, mitochondrial translation, mitochondrion organization RNA binding, GTPase activity, hydrolase activity, translation factor activity, RNA binding mitochondrion, cytoplasm 1714 BY4741 15_5 8 A 4 YOL024W - - Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication biological_process molecular_function cellular_component 1715 BY4741 15_5 8 A 5 YOL025W LAG2 ECM36 Protein that negatively regulates the SCF E3-ubiquitin ligase; regulates by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 regulation of protein modification process, protein modification by small protein conjugation or removal molecular_function mitochondrion, cytoplasm 1716 BY4741 15_5 8 A 6 YOL027C MDM38 ribosome-binding protein MDM38|MKH1 Mitochondrial protein; forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, ion transport, protein complex biogenesis, cellular ion homeostasis other mitochondrion, membrane, mitochondrial envelope, cytoplasm 1718 BY4741 15_5 8 A 7 YOL028C YAP7 - Putative basic leucine zipper (bZIP) transcription factor; YAP7 has a paralog, YAP5, that arose from the whole genome duplication transcription from RNA polymerase II promoter DNA binding nucleus 1719 BY4741 15_5 8 A 9 YOL029C - - Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens biological_process molecular_function cellular_component 1720 BY4741 15_5 8 A 10 YOL030W GAS5 1,3-beta-glucanosyltransferase 1,3-beta-glucanosyltransferase; has similarity to Gas1p; localizes to the cell wall carbohydrate metabolic process transferase activity cell wall, membrane 1721 BY4741 15_5 8 A 11 YOL031C SIL1 SLS1 Nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; required for protein translocation into the endoplasmic reticulum (ER); homolog of Yarrowia lipolytica SLS1; GrpE-like protein protein targeting, transmembrane transport enzyme regulator activity endomembrane system, endoplasmic reticulum, cytoplasm 1722 BY4741 15_5 8 A 12 YOL032W OPI10 - Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress carbohydrate metabolic process molecular_function nucleus, cytoplasm 1723 BY4741 15_5 8 B 1 YOL033W MSE1 glutamate--tRNA ligase MSE1 Mitochondrial glutamyl-tRNA synthetase; predicted to be palmitoylated mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 1724 BY4741 15_5 8 B 2 YOL035C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1726 BY4741 15_5 8 B 3 YOL036W - - Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication biological_process molecular_function cellular_component 1727 BY4741 15_5 8 B 4 YOL037C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W biological_process molecular_function cellular_component 1728 BY4741 15_5 8 B 5 YOL039W RPP2A ribosomal protein P2A|RPLA2|RPL44 Ribosomal protein P2 alpha; a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P2 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm cytoplasmic translation structural molecule activity, structural constituent of ribosome, enzyme regulator activity ribosome, cytoplasm 1730 BY4741 15_5 8 B 6 YOL041C NOP12 - Nucleolar protein involved in pre-25S rRNA processing; also involved in biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p rRNA processing, ribosomal large subunit biogenesis RNA binding nucleus, nucleolus 1732 BY4741 15_5 8 B 7 YOL042W NGL1 RNA exonuclease Putative endonuclease; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process nuclease activity, hydrolase activity mitochondrion, cytoplasm 1733 BY4741 15_5 8 B 8 YOL043C NTG2 bifunctional N-glycosylase/AP lyase NTG2|SCR2 DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication DNA repair, cellular response to DNA damage stimulus lyase activity, nuclease activity, hydrolase activity, hydrolase activity, acting on glycosyl bonds nucleus 1734 BY4741 15_5 8 B 9 YOL044W PEX15 PAS21 Tail-anchored type II integral peroxisomal membrane protein; required for peroxisome biogenesis; cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol; overexpression results in impaired peroxisome assembly protein targeting, transmembrane transport, peroxisome organization, signaling other peroxisome, membrane, cytoplasm 1735 BY4741 15_5 8 B 10 YOL045W PSK2 serine/threonine protein kinase PSK2 PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication cytoplasmic translation, carbohydrate metabolic process, regulation of translation, protein phosphorylation, generation of precursor metabolites and energy kinase activity, transferase activity mitochondrion, cytoplasm 1736 BY4741 15_5 8 B 11 YOL046C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene PSK2/YOL045W biological_process molecular_function cellular_component 1737 BY4741 15_5 8 B 12 YOL047C LDS2 - Protein Involved in spore wall assembly; localizes to lipid droplets found on or outside of the prospore membrane; shares similarity with Lds1p and Rrt8p, and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function cell wall, membrane, cytoplasm 1738 BY4741 15_5 8 C 1 YOL048C RRT8 - Protein involved in spore wall assembly; shares similarity with Lds1p and Lds2p and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function cell wall, membrane 1739 BY4741 15_5 8 C 2 YOL049W GSH2 glutathione synthase Glutathione synthetase; catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock other ligase activity other 1740 BY4741 15_5 8 C 3 YOL050C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) biological_process molecular_function cellular_component 1741 BY4741 15_5 8 C 4 YOL051W GAL11 MED15|ABE1|SPT13|SDS4|RAR3 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator invasive growth in response to glucose limitation, DNA-templated transcription, initiation, transcription from RNA polymerase II promoter transcription factor activity, protein binding, transcription factor binding nucleus 1742 BY4741 15_5 8 C 5 YOL052C SPE2 adenosylmethionine decarboxylase SPE2 S-adenosylmethionine decarboxylase; required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically cofactor metabolic process, vitamin metabolic process, monocarboxylic acid metabolic process lyase activity nucleus, cytoplasm 1743 BY4741 15_5 8 C 6 YOL053C-A nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - 1744 BY4741 15_5 8 C 7 YOL053W AIM39 - Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 1745 BY4741 15_5 8 C 8 YOL054W PSH1 ubiquitin-protein ligase PSH1 E3 ubiquitin ligase targeting centromere-binding protein Cse4p; mediates poyubiquitination and degradation of Cse4p; prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process transferase activity nucleus, chromosome 1746 BY4741 15_5 8 C 9 YOL055C THI20 trifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase/thiaminase Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p vitamin metabolic process kinase activity, transferase activity, hydrolase activity cytoplasm 1747 BY4741 15_5 8 C 10 YOL056W GPM3 phosphoglycerate mutase family protein GPM3 Homolog of Gpm1p phosphoglycerate mutase; converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional; GPM3 has a paralog, GPM2, that arose from the whole genome duplication biological_process molecular_function cytoplasm 1748 BY4741 15_5 8 C 11 YOL057W - dipeptidyl-peptidase III Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers other peptidase activity, hydrolase activity nucleus, cytoplasm 1749 BY4741 15_5 8 C 12 YOL058W ARG1 argininosuccinate synthase|ARG10 Arginosuccinate synthetase; catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate cellular amino acid metabolic process ligase activity cytoplasm 1750 BY4741 15_5 8 D 1 YOL059W GPD2 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD2|GPD3 NAD-dependent glycerol 3-phosphate dehydrogenase; expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1; GPD2 has a paralog, GPD1, that arose from the whole genome duplication carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity mitochondrion, cytoplasm 1751 BY4741 15_5 8 D 2 YOL060C MAM3 - Protein required for normal mitochondrial morphology; has similarity to hemolysins cellular ion homeostasis, mitochondrion organization molecular_function membrane, vacuole, cytoplasm 1752 BY4741 15_5 8 D 3 YOL061W PRS5 ribose phosphate diphosphokinase subunit PRS5 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress cell wall organization or biogenesis transferase activity cytoplasm 1753 BY4741 15_5 8 D 4 YOL062C APM4 AMP1 Cargo-binding mu subunit of AP-2; AP-2 is a heterotetrameric endocytic cargo-binding adaptor that facilitates uptake of membrane proteins during clathrin-mediated endocytosis; Apm4p is required for AP-2 function and localization, and binds cell wall stress receptor Mid2p; AP-2 is required for cell polarity responses to pheromone, nutritional status and cell wall damage in S. cerevisiae, and for hyphal growth in C. albicans; AP-2 complex is conserved in mammals other other membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, plasma membrane, cytoplasm 1754 BY4741 15_5 8 D 5 YOL063C CRT10 HUS1 Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat transcription from RNA polymerase II promoter molecular_function cellular_component 1755 BY4741 15_5 8 D 6 YOL064C MET22 3'(2'),5'-bisphosphate nucleotidase|HAL2 Bisphosphate-3'-nucleotidase; involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway; human homolog BPNT1 complements yeast null mutant response to osmotic stress, cellular amino acid metabolic process phosphatase activity, hydrolase activity cellular_component 1756 BY4741 15_5 8 D 7 YOL065C INP54 phosphoinositide 5-phosphatase INP54 Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; role in secretion; localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins lipid metabolic process phosphatase activity, hydrolase activity endomembrane system, endoplasmic reticulum, cytoplasm 1757 BY4741 15_5 8 D 8 YOL067C RTG1 - Transcription factor (bHLH) involved in interorganelle communication; contributes to communication between mitochondria, peroxisomes, and nucleus; target of Hog1p; activated in stochastic pulses of nuclear localization response to chemical, transcription from RNA polymerase II promoter, signaling nucleic acid binding transcription factor activity nucleus, cytoplasm 1759 BY4741 15_5 8 D 9 YOL068C HST1 histone deacetylase HST1 NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance; HST1 has a paralog, SIR2, that arose from the whole genome duplication chromatin organization, DNA recombination, regulation of DNA metabolic process, histone modification hydrolase activity nucleus, chromosome 1760 BY4741 15_5 8 D 10 YOL070C NBA1 - Protein of unknown function; localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate biological_process molecular_function cellular bud, site of polarized growth, ribosome, cytoplasm 1762 BY4741 15_5 8 D 11 YOL071W SDH5 EMI5|succinate dehydrogenase assembly factor SDH5 Protein required for flavinylation of Sdh1p; binds to Sdh1p and promotes FAD cofactor attachment, which is necessary for succinate dehydrogenase (SDH) complex assembly and activity; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma) meiotic cell cycle, sporulation, mitochondrion organization, generation of precursor metabolites and energy, protein complex biogenesis, cellular respiration, transcription from RNA polymerase II promoter molecular_function mitochondrion, cytoplasm 1763 BY4741 15_5 8 D 12 YOL072W THP1 BUD29 Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding mitotic cell cycle, DNA repair, cytokinesis, cellular response to DNA damage stimulus, nucleobase-containing compound transport, RNA catabolic process, mRNA processing, transcription from RNA polymerase II promoter, nuclear transport, DNA-templated transcription, elongation RNA binding, DNA binding nucleus, endomembrane system 1764 BY4741 15_5 8 E 1 YOL075C - - Putative ABC transporter other ATPase activity, hydrolase activity, transmembrane transporter activity membrane, vacuole, cytoplasm 1766 BY4741 15_5 8 E 2 YOL076W MDM20 NAA25|DEC1 Non-catalytic subunit of the NatB N-terminal acetyltransferase; NatB catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly peptidyl-amino acid modification, protein acylation, organelle inheritance, mitochondrion organization, cytoskeleton organization, regulation of organelle organization transferase activity cytoplasm 1767 BY4741 15_5 8 E 3 YOL079W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane 1770 BY4741 15_5 8 E 4 YOL080C REX4 - Putative RNA exonuclease; possibly involved in pre-rRNA processing and ribosome assembly ribosome assembly, rRNA processing, organelle assembly, ribosomal large subunit biogenesis nuclease activity, hydrolase activity nucleus, nucleolus 1771 BY4741 15_5 8 E 5 YOL081W IRA2 Ras GTPase activating protein IRA2|GLC4|CCS1 GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; IRA2 has a paralog, IRA1, that arose from the whole genome duplication; defects in human homolog NF1 are associated with neurofibromatosis response to chemical, response to oxidative stress, nucleobase-containing small molecule metabolic process, signaling enzyme regulator activity endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm 1772 BY4741 15_5 8 E 6 YOL082W ATG19 CVT19 Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles; interaction with Atg19p during the Cvt pathway requires phosphorylation by Hrr25p response to chemical, protein targeting, vesicle organization, proteolysis involved in cellular protein catabolic process, protein maturation protein binding, bridging membrane, cytoplasm 1773 BY4741 15_5 8 E 7 YOL083W ATG34 ATG19-B Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog protein targeting, protein complex biogenesis protein binding, bridging cytoplasm 1774 BY4741 15_5 8 E 8 YOL084W PHM7 - Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress biological_process molecular_function membrane, vacuole, cytoplasm 1775 BY4741 15_5 8 E 9 YOL085C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A biological_process molecular_function cellular_component 1776 BY4741 15_5 8 E 10 YPL274W SAM3 bifunctional polyamine/amino acid permease SAM3 High-affinity S-adenosylmethionine permease; required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p ion transport, nucleobase-containing compound transport transmembrane transporter activity membrane, plasma membrane 1018 BY4741 16_1 8 E 11 YPL273W SAM4 S-adenosylmethionine-homocysteine S-methyltransferase SAM4 S-adenosylmethionine-homocysteine methyltransferase; functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio; SAM4 has a paralog, YMR321C, that arose from a single-locus duplication cofactor metabolic process, nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process transferase activity, methyltransferase activity nucleus, cytoplasm 1019 BY4741 16_1 8 E 12 YPL272C PBI1 - Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene biological_process molecular_function cellular_component 1020 BY4741 16_1 8 F 1 YPL271W ATP15 F1F0 ATP synthase subunit epsilon|ATPEPSILON Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated transmembrane transport, ion transport, nucleobase-containing small molecule metabolic process hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1021 BY4741 16_1 8 F 2 YPL270W MDL2 ATP-binding cassette permease MDL2 Mitochondrial inner membrane half-type ABC transporter; required for respiratory growth at high temperature; localizes to vacuole membrane in response to H2O2; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis response to chemical, transmembrane transport, response to oxidative stress hydrolase activity, transmembrane transporter activity, ATPase activity vacuole, mitochondrion, membrane, mitochondrial envelope, cytoplasm 1022 BY4741 16_1 8 F 3 YPL269W KAR9 - Karyogamy protein; required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules; localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, proteolysis involved in cellular protein catabolic process, chromosome segregation, regulation of cell cycle molecular_function cell cortex, cytoskeleton, microtubule organizing center, site of polarized growth, cytoplasm 1023 BY4741 16_1 8 F 4 YPL267W ACM1 - Pseudosubstrate inhibitor of the APC/C; suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein; the anaphase-promoting complex/cyclosome is also known as APC/C mitotic cell cycle, regulation of protein modification process, protein modification by small protein conjugation or removal enzyme regulator activity nucleus, cytoplasm 1025 BY4741 16_1 8 F 5 YPL265W DIP5 - Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress ion transport, amino acid transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm 1027 BY4741 16_1 8 F 6 YPL264C - - Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene biological_process molecular_function membrane 1028 BY4741 16_1 8 F 7 YPL263C KEL3 - Cytoplasmic protein of unknown function biological_process molecular_function cytoplasm 1029 BY4741 16_1 8 F 8 YPL262W FUM1 fumarase FUM1 Fumarase; converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria generation of precursor metabolites and energy, cellular respiration lyase activity mitochondrion, cytoplasm 1030 BY4741 16_1 8 F 9 YPL260W - - Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus, cytoplasm 1031 BY4741 16_1 8 F 10 YPL261C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YPL260W biological_process molecular_function cellular_component 1032 BY4741 16_1 8 F 11 YPL259C APM1 YAP54 Mu1-like medium subunit of the AP-1 complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; the AP-1 complex is the clathrin-associated protein complex Golgi vesicle transport other membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 1033 BY4741 16_1 8 F 12 YPL258C THI21 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p vitamin metabolic process kinase activity, transferase activity cellular_component 1034 BY4741 16_1 8 G 1 YPL257W - - Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene biological_process molecular_function membrane 1035 BY4741 16_1 8 G 2 YPL256C CLN2 cyclin CLN2 G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN2 has a paralog, CLN1, that arose from the whole genome duplication; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1 response to chemical, protein phosphorylation, regulation of protein modification process, conjugation, signaling, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm 1036 BY4741 16_1 8 G 3 YPL254W HFI1 GAN1|SRM12|SUP110|ADA1 Adaptor protein required for structural integrity of the SAGA complex; a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions peptidyl-amino acid modification, protein acylation, chromatin organization, transcription from RNA polymerase II promoter, histone modification transcription factor activity, protein binding nucleus 1038 BY4741 16_1 8 G 4 YPL253C VIK1 - Protein that forms a kinesin-14 heterodimeric motor with Kar3p; localizes Kar3p at mitotic spindle poles; has a structure similar to a kinesin motor domain but lacks an ATP-binding site and is catalytically inactive; binds microtubules; required for sister chromatid cohesion; VIK1 has a paralog, CIK1, that arose from the whole genome duplication mitotic cell cycle, organelle fission, chromosome segregation cytoskeletal protein binding cytoskeleton, microtubule organizing center 1039 BY4741 16_1 8 G 5 YPL250C ATG41 ICY2 Protein of unknown function; required for selective and nonselective autophagy, and mitophagy; regulates the rate of autophagosome formation; interacts with Atg9p, and has a similar peri-mitochondrial localization; elevated Gcn4p-dependent expression under autophagy-inducing conditions; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate; ATG41 has a paralog, ICY1, that arose from the whole genome duplication vacuole organization, protein targeting, mitochondrion organization, organelle assembly molecular_function cellular_component 1042 BY4741 16_1 8 G 6 YPL249C GYP5 - GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; GYP5 has a paralog, GYL1, that arose from the whole genome duplication Golgi vesicle transport enzyme regulator activity endomembrane system, cellular bud, cytoplasmic membrane-bounded vesicle, membrane, site of polarized growth, Golgi apparatus, cytoplasm, plasma membrane 1043 BY4741 16_1 8 G 7 YPL248C GAL4 galactose-responsive transcription factor GAL4|GAL81 DNA-binding transcription factor required for activating GAL genes; responds to galactose; repressed by Gal80p and activated by Gal3p carbohydrate metabolic process, response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding, transcription factor binding nucleus 1044 BY4741 16_1 8 G 8 YPL247C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest biological_process molecular_function nucleus, cytoplasm 1045 BY4741 16_1 8 G 9 YPL246C RBD2 putative rhomboid protease RBD2 Possible rhomboid protease; has similarity to eukaryotic rhomboid proteases including Pcp1p biological_process molecular_function nucleus, membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 1046 BY4741 16_1 8 G 10 YPL245W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm biological_process molecular_function nucleus, cytoplasm 1047 BY4741 16_1 8 G 11 YPL244C HUT1 - Protein with a role in UDP-galactose transport to the Golgi lumen; has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 ion transport, nucleobase-containing compound transport transmembrane transporter activity membrane 1048 BY4741 16_1 8 G 12 YPL241C CIN2 GTPase-activating protein CIN2 GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; human homolog RP2 complements yeast null mutant protein folding, protein complex biogenesis enzyme regulator activity cellular_component 1051 BY4741 16_1 8 H 1 YPL240C HSP82 Hsp90 family chaperone HSP82|HSP90 Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication telomere organization, protein targeting, DNA replication, mitochondrion organization, regulation of organelle organization, protein complex biogenesis, protein folding, regulation of DNA metabolic process, response to osmotic stress hydrolase activity, unfolded protein binding, ATPase activity cytoplasm 1052 BY4741 16_1 8 H 3 YPL239W YAR1 - Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with nascent Rps3p during its translation in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock response to chemical, ribosomal subunit export from nucleus, ribosomal small subunit biogenesis, response to oxidative stress, nuclear transport, response to osmotic stress unfolded protein binding nucleus, cytoplasm 1053 BY4741 16_1 8 H 4 YPL236C ENV7 putative serine/threonine protein kinase ENV7 Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) vacuole organization, regulation of organelle organization, organelle fusion, protein maturation, membrane fusion kinase activity, transferase activity vacuole, membrane, cytoplasm 1056 BY4741 16_1 8 H 5 YPL234C VMA11 H(+)-transporting V0 sector ATPase subunit c'|TFP3|CLS9 Vacuolar ATPase V0 domain subunit c'; involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen cellular ion homeostasis ATPase activity, hydrolase activity, transmembrane transporter activity membrane, vacuole, cytoplasm 1058 BY4741 16_1 8 H 6 YPL232W SSO1 - Plasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p; SSO1 has a paralog, SSO2, that arose from the whole genome duplication meiotic cell cycle, sporulation, organelle fusion, vesicle organization, membrane fusion lipid binding, ion binding vacuole, membrane, cytoplasm, plasma membrane 1060 BY4741 16_1 8 H 7 YPL230W USV1 NSF1 Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication response to chemical, transcription from RNA polymerase II promoter, response to osmotic stress DNA binding nucleus 1062 BY4741 16_1 8 H 8 YPL229W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication biological_process molecular_function cytoplasm 1063 BY4741 16_1 8 H 9 YPL227C ALG5 dolichyl-phosphate beta-glucosyltransferase UDP-glucose:dolichyl-phosphate glucosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum; human ortholog ALG5 can partially complement yeast alg5 mutant carbohydrate metabolic process, protein glycosylation transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 1065 BY4741 16_1 8 H 10 YPL226W NEW1 - ATP binding cassette protein; cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction nucleobase-containing compound transport, ribosomal small subunit biogenesis, nuclear transport RNA binding, hydrolase activity, ion binding, ATPase activity, mRNA binding mitochondrion, cytoplasm 1066 BY4741 16_1 8 H 11 YPL225W - - Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress biological_process molecular_function ribosome, cytoplasm 1067 BY4741 16_1 8 H 12 YPL223C GRE1 - Hydrophilin essential in desiccation-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; GRE1 has a paralog, SIP18, that arose from the whole genome duplication biological_process molecular_function cytoplasm 1069 BY4741 16_1 9 A 1 YPL222W FMP40 - Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 1070 BY4741 16_1 9 A 2 YPL221W FLC1 flavin adenine dinucleotide transporter FLC1|BOP1|HUF1 Flavin adenine dinucleotide transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC1 has a paralog, FLC3, that arose from the whole genome duplication ion transport, nucleobase-containing compound transport, protein folding, cell wall organization or biogenesis transmembrane transporter activity endomembrane system, vacuole, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm 1071 BY4741 16_1 9 A 3 YPL220W RPL1A PUB2|ribosomal 60S subunit protein L1A|L1|L1A|SSM1 Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 1072 BY4741 16_1 9 A 4 YPL219W PCL8 - Cyclin; interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation; PCL8 has a paralog, PCL10, that arose from the whole genome duplication carbohydrate metabolic process, generation of precursor metabolites and energy enzyme regulator activity other 1073 BY4741 16_1 9 A 5 YPL216W - - Putative protein of unknown function; not an essential gene; YPL216W has a paralog, ITC1, that arose from the whole genome duplication biological_process molecular_function cellular_component 1076 BY4741 16_1 9 A 6 YPL215W CBP3 - Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other vacuole, ribosome, mitochondrion, membrane, mitochondrial envelope, cytoplasm 1077 BY4741 16_1 9 A 7 YPL214C THI6 bifunctional hydroxyethylthiazole kinase/thiamine-phosphate diphosphorylase Thiamine-phosphate diphosphorylase and hydroxyethylthiazole kinase; required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern vitamin metabolic process kinase activity, transferase activity cytoplasm 1078 BY4741 16_1 9 A 8 YPL213W LEA1 U2 snRNP complex subunit LEA1 Component of U2 snRNP complex; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein RNA splicing, mRNA processing molecular_function nucleus, cytoplasm 1079 BY4741 16_1 9 A 10 YPL212C PUS1 pseudouridine synthase PUS1 tRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; also acts on U2 snRNA; also pseudouridylates some mRNAs, and pseudouridylation level varies with growth phase; nuclear protein that appears to be involved in tRNA export; PUS1 has a paralog, PUS2, that arose from the whole genome duplication tRNA processing, RNA modification RNA binding, mRNA binding, isomerase activity nucleus 1080 BY4741 16_1 9 A 11 YPL208W RKM1 - SET-domain lysine-N-methyltransferase; catalyzes the formation of dimethyllysine residues on the large ribosomal subunit proteins L23 (Rpl23Ap and Rpl23Bp) and monomethyllysine residues on L18 (Rps18Ap and Rps18Bp) peptidyl-amino acid modification, protein alkylation transferase activity, methyltransferase activity nucleus, cytoplasm 1084 BY4741 16_1 9 A 12 YPL207W TYW1 - Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron tRNA processing, RNA modification molecular_function endomembrane system, endoplasmic reticulum, cytoplasm 1085 BY4741 16_1 9 B 1 YPL206C PGC1 - Phosphatidylglycerol phospholipase C; regulates phosphatidylglycerol (PG) accumulation via a phospholipase C-type degradation mechanism; PG levels affect mitochondrial function; contains glycerophosphodiester phosphodiesterase motifs lipid metabolic process hydrolase activity mitochondrion, cytoplasm 1086 BY4741 16_1 9 B 2 YPL205C - - Hypothetical protein; deletion of locus affects telomere length meiotic cell cycle, sporulation, transcription from RNA polymerase II promoter molecular_function cellular_component 1087 BY4741 16_1 9 B 3 YPL203W TPK2 cAMP-dependent protein kinase catalytic subunit TPK2|PKA3|YKR1|PKA2 cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase; relocalizes to the cytosol in response to hypoxia protein phosphorylation, invasive growth in response to glucose limitation, signaling kinase activity, transferase activity nucleus, chromosome, cytoplasm 1089 BY4741 16_1 9 B 4 YPL202C AFT2 - Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication response to chemical, response to oxidative stress, cellular ion homeostasis, response to starvation, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, mitochondrion, cytoplasm 1090 BY4741 16_1 9 B 5 YPL201C YIG1 - Protein that interacts with glycerol 3-phosphatase; plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol carbohydrate metabolic process molecular_function nucleus, cytoplasm 1091 BY4741 16_1 9 B 6 YPL200W CSM4 - Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements; functions with meiosis-specific telomere-binding protein Ndj1p; CSM4 has a paralog, MPS2, that arose from the whole genome duplication meiotic cell cycle, organelle fission, regulation of organelle organization, chromosome segregation, DNA recombination, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, endomembrane system, chromosome, membrane, endoplasmic reticulum, cytoplasm 1092 BY4741 16_1 9 B 7 YPL199C - - Putative protein of unknown function; predicted to be palmitoylated biological_process molecular_function cytoplasm 1093 BY4741 16_1 9 B 8 YPL198W RPL7B ribosomal 60S subunit protein L7B|YL8|L7B|L6B|L30|rp11 Ribosomal 60S subunit protein L7B; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); binds to Domain II of 25S and 5.8S rRNAs; homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal large subunit biogenesis structural constituent of ribosome, structural molecule activity nucleus, ribosome, nucleolus, cytoplasm 1094 BY4741 16_1 9 B 9 YPL197C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the ribosomal gene RPL7B biological_process molecular_function cellular_component 1095 BY4741 16_1 9 B 10 YPL196W OXR1 - Protein of unknown function required for oxidative damage resistance; required for normal levels of resistance to oxidative damage; null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes response to chemical, response to oxidative stress molecular_function mitochondrion, cytoplasm 1096 BY4741 16_1 9 B 11 YPL195W APL5 YKS4 Delta adaptin-like subunit of the clathrin associated protein complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; suppressor of loss of casein kinase 1 function; the clathrin associated protein complex is also known as AP-3 Golgi vesicle transport, protein targeting other membrane, cytoplasm 1097 BY4741 16_1 9 B 12 YPL194W DDC1 - DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress mitotic cell cycle, meiotic cell cycle, DNA repair, organelle fission, regulation of organelle organization, cellular response to DNA damage stimulus, DNA recombination, regulation of cell cycle molecular_function nucleus, chromosome 1098 BY4741 16_1 9 C 1 YPL193W RSA1 - Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly ribosome assembly, organelle assembly, ribosomal large subunit biogenesis molecular_function nucleus 1099 BY4741 16_1 9 C 2 YPL192C PRM3 pheromone-regulated protein PRM3 Protein required for nuclear envelope fusion during karyogamy; pheromone-regulated; peripheral protein of the nuclear membrane; interacts with Kar5p at the spindle pole body membrane fusion, conjugation, organelle fusion, nucleus organization molecular_function nucleus, membrane, cytoskeleton, microtubule organizing center, endomembrane system 1100 BY4741 16_1 9 C 3 YPL191C - - Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL191C has a paralog, YGL082W, that arose from the whole genome duplication biological_process molecular_function cytoplasm 1101 BY4741 16_1 9 C 4 YPL189W GUP2 - Probable membrane protein; possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; homolog of the mammalian Hedgehog pathway modulator HHAT; GUP2 has a paralog, GUP1, that arose from the whole genome duplication carbohydrate transport transferase activity membrane 1103 BY4741 16_1 9 C 5 YPL188W POS5 NADH kinase Mitochondrial NADH kinase; phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress response to chemical, cofactor metabolic process, response to oxidative stress, nucleobase-containing small molecule metabolic process kinase activity, transferase activity mitochondrion, cytoplasm 1104 BY4741 16_1 9 C 6 YPL187W MF(ALPHA)1 - Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor; MF(ALPHA)1 has a paralog, MF(ALPHA)2, that arose from the whole genome duplication response to chemical, conjugation, signaling other extracellular region 2065 BY4741 16_2 9 C 7 YPL185W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C biological_process molecular_function membrane 2066 BY4741 16_2 9 C 8 YPL186C UIP4 - Protein that interacts with Ulp1p; a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope biological_process molecular_function nucleus, membrane, endomembrane system, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm 2067 BY4741 16_2 9 C 9 YPL184C MRN1 PTR69 RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation RNA splicing, chromatin organization, mRNA processing RNA binding, mRNA binding nucleus, cytoplasm 2068 BY4741 16_2 9 C 10 YPL181W CTI6 RXT1 Component of the Rpd3L histone deacetylase complex; relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain chromatin organization, DNA replication, regulation of organelle organization, regulation of DNA metabolic process histone binding, transcription factor binding nucleus, chromosome 2070 BY4741 16_2 9 C 11 YPL182C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W biological_process molecular_function cellular_component 2071 BY4741 16_2 9 C 12 YPL180W TCO89 - Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p); regulates global H3K56ac; TORC1 complex regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin carbohydrate metabolic process, cell wall organization or biogenesis, signaling, response to osmotic stress molecular_function vacuole, membrane, cytoplasm, plasma membrane 2072 BY4741 16_2 9 D 1 YPL179W PPQ1 SAL6 Protein phosphatase that regulates the mating response; negatively regulates the MAP kinase signaling cascade during mating; member of the serine/threonine phosphatase PP1 family response to chemical, protein dephosphorylation, conjugation, signaling phosphatase activity, hydrolase activity cytoplasm 2073 BY4741 16_2 9 D 2 YPL178W CBC2 CBP20|SAE1|MUD13 Small subunit of the heterodimeric cap binding complex with Sto1p; interacts with Npl3p, possibly to package mRNA for export from the nucleus; may have a role in telomere maintenance; contains an RNA-binding motif RNA splicing, mRNA processing RNA binding nucleus 2074 BY4741 16_2 9 D 3 YPL177C CUP9 - Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress regulation of transport, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding, transcription factor binding nucleus 2075 BY4741 16_2 9 D 4 YPL176C TRE1 - Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication protein targeting, endosomal transport, proteolysis involved in cellular protein catabolic process molecular_function vacuole, cytoplasm 2076 BY4741 16_2 9 D 5 YPL174C NIP100 PAC13 Large subunit of the dynactin complex; dynactin is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) mitotic cell cycle, cytoskeleton organization cytoskeletal protein binding cell cortex, cytoskeleton, microtubule organizing center, cytoplasm 2078 BY4741 16_2 9 D 6 YPL173W MRPL40 mitochondrial 54S ribosomal protein YmL40|YmL40 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 2079 BY4741 16_2 9 D 7 YPL172C COX10 protoheme IX farnesyltransferase Heme A:farnesyltransferase; catalyzes first step in conversion of protoheme to heme A prosthetic group required for cytochrome c oxidase activity; human ortholog COX10 can complement yeast cox10 null mutant; human ortholog COX10 is associated with mitochondrial disorders cofactor metabolic process transferase activity membrane, mitochondrion, cytoplasm 2080 BY4741 16_2 9 D 8 YPL171C OYE3 ZRG6 Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death other oxidoreductase activity cellular_component 2081 BY4741 16_2 9 D 9 YPL170W DAP1 - Heme-binding protein; involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis lipid metabolic process other membrane, endomembrane system, cytoplasm 2082 BY4741 16_2 9 D 10 YPL168W MRX4 - Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated biological_process molecular_function mitochondrion, cytoplasm 2084 BY4741 16_2 9 D 11 YPL167C REV3 PSO1 Catalytic subunit of DNA polymerase zeta; involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair; forms a complex with Rev7p, Pol31p and Pol32p DNA repair, cellular response to DNA damage stimulus transferase activity, nucleotidyltransferase activity nucleus, chromosome, mitochondrion, cytoplasm 2085 BY4741 16_2 9 D 12 YPL166W ATG29 - Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress membrane invagination, mitochondrion organization molecular_function nucleus, cytoplasm 2086 BY4741 16_2 9 E 1 YPL165C SET6 - SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability biological_process molecular_function cellular_component 2087 BY4741 16_2 9 E 2 YPL164C MLH3 mismatch repair protein MLH3 Protein involved in DNA mismatch repair and meiotic recombination; involved in crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability meiotic cell cycle, DNA repair, organelle fission, DNA recombination, cellular response to DNA damage stimulus ATPase activity, hydrolase activity nucleus 2088 BY4741 16_2 9 E 3 YPL163C SVS1 - Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication response to chemical molecular_function cell wall, vacuole, cytoplasm 2089 BY4741 16_2 9 E 4 YPL162C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology biological_process molecular_function membrane, vacuole, cytoplasm 2090 BY4741 16_2 9 E 5 YPL161C BEM4 ROM7 Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length cytoskeleton organization, signaling molecular_function nucleus, cytoplasm 2091 BY4741 16_2 9 E 6 YPL159C PET20 - Mitochondrial protein; required for respiratory growth under some conditions and for stability of the mitochondrial genome generation of precursor metabolites and energy, cellular respiration molecular_function mitochondrion, cytoplasm 2093 BY4741 16_2 9 E 7 YPL157W TGS1 - Trimethyl guanosine synthase, conserved nucleolar methyl transferase; converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs; interacts with Swm2p, which may confer substrate specificity on Tgs1p meiotic cell cycle, telomere organization, organelle fission, DNA replication, regulation of organelle organization, regulation of DNA metabolic process, RNA modification, nucleus organization transferase activity, methyltransferase activity nucleus, nucleolus 2095 BY4741 16_2 9 E 8 YPL156C PRM4 pheromone-regulated protein PRM4 Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift biological_process molecular_function membrane 2096 BY4741 16_2 9 E 9 YPL155C KIP2 - Kinesin-related motor protein involved in mitotic spindle positioning; stabilizes microtubules by targeting Bik1p to the plus end; functions as a microtubule polymerase and catastrophe inhibitor in vitro; Kip2p levels are controlled during the cell cycle cytoskeleton organization, regulation of organelle organization, protein complex biogenesis hydrolase activity cytoskeleton, mitochondrion, cytoplasm 2097 BY4741 16_2 9 E 10 YPL154C PEP4 proteinase A|yscA|PRA1|PHO9 Vacuolar aspartyl protease (proteinase A); required for posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; plays a protective role in acetic acid induced apoptosis; synthesized as a zymogen, self-activates protein targeting, proteolysis involved in cellular protein catabolic process, membrane invagination, response to starvation peptidase activity, hydrolase activity vacuole, mitochondrion, cytoplasm 2098 BY4741 16_2 9 E 11 YPL152W RRD2 peptidylprolyl isomerase RRD2|YPA2 Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress mitotic cell cycle, cytoskeleton organization, response to osmotic stress isomerase activity, enzyme regulator activity other 2100 BY4741 16_2 9 E 12 YPL150W - non-specific serine/threonine protein kinase Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study protein phosphorylation kinase activity, transferase activity cellular_component 2102 BY4741 16_2 9 F 1 YPL149W ATG5 APG5 Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; Atg5p-Atg12p conjugate also forms a complex with Atg16p; the Atg5-Atg12/Atg16 complex binds to membranes and is essential for autophagosome formation; also involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner protein targeting, mitochondrion organization, membrane invagination, protein lipidation transferase activity, enzyme regulator activity vacuole, membrane, cytoplasm 2103 BY4741 16_2 9 F 2 YPL147W PXA1 ATP-binding cassette long-chain fatty acid transporter PXA1|SSH2|PAT2|PAL1|LPI1 Subunit of heterodimeric peroxisomal ABC transport complex, with Pxa2p; required for import of long-chain fatty acids into peroxisomes; similar to human adrenoleukodystrophy transporters ABCD1and ABCD2, and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder; human ABCD1 and ABCD2 can each partially complement yeast pxa1 pxa2 double null mutant ion transport, lipid transport hydrolase activity, transmembrane transporter activity, ATPase activity peroxisome, membrane, cytoplasm 2105 BY4741 16_2 9 F 3 YPL145C KES1 oxysterol-binding protein KES1|BSR3|OSH4|LPI3 One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication protein dephosphorylation, Golgi vesicle transport, regulation of protein modification process, lipid transport, lipid metabolic process, membrane invagination, exocytosis, endocytosis lipid binding, ion binding endomembrane system, membrane, Golgi apparatus, cytoplasm 2107 BY4741 16_2 9 F 4 YPL144W POC4 DMP1|PBA4 Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions; upregulates proteasome assembly in response to the unfolded protein response activated by mistargeting of proteins (UPRam) protein complex biogenesis molecular_function cytoplasm 2108 BY4741 16_2 9 F 5 YPL141C FRK1 protein kinase FRK1 Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication protein phosphorylation kinase activity, transferase activity cytoplasm 2111 BY4741 16_2 9 F 6 YPL140C MKK2 putative mitogen-activated protein kinase kinase MKK2|SSP33|LPI6 MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication protein phosphorylation, peroxisome organization, cell wall organization or biogenesis, signaling kinase activity, transferase activity cellular bud, site of polarized growth, cytoplasm 2112 BY4741 16_2 9 F 7 YPL139C UME1 WTM3 Component of both the Rpd3S and Rpd3L histone deacetylase complexes; negative regulator of meiosis; required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p; UME1 has a paralog, WTM2, that arose from the whole genome duplication transcription from RNA polymerase I promoter, meiotic cell cycle, organelle fission, chromatin organization, regulation of organelle organization, transcription from RNA polymerase II promoter, regulation of cell cycle transcription factor activity, protein binding nucleus, chromosome, cytoplasm 2113 BY4741 16_2 9 F 8 YPL138C SPP1 SAF41|CPS40 Subunit of COMPASS (Set1C); a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein; relocalizes to the cytosol in response to hypoxia peptidyl-amino acid modification, protein alkylation, chromatin organization, histone modification chromatin binding, transferase activity, histone binding, methyltransferase activity nucleus, cytoplasm 2114 BY4741 16_2 9 F 9 YPL136W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C biological_process molecular_function cellular_component 2115 BY4741 16_2 9 F 10 YPL135W ISU1 iron-binding protein ISU1|NUA1 Conserved protein of the mitochondrial matrix; performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); ISU1 has a paralog, ISU2, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant cellular ion homeostasis, tRNA processing, RNA modification ion binding mitochondrion, cytoplasm 2117 BY4741 16_2 9 F 11 YPL133C RDS2 - Transcription factor involved in regulating gluconeogenesis; also involved in the regulation of glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole carbohydrate metabolic process, response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 2119 BY4741 16_2 9 F 12 YPL130W SPO19 - Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation; SPO19 has a paralog, YOR214C, that arose from the whole genome duplication meiotic cell cycle, organelle fission, sporulation molecular_function cell wall, membrane 2122 BY4741 16_2 9 G 1 YPL129W TAF14 TATA-binding protein-associated factor TAF14|TafII30|ANC1|TFG3|TAF30|SWP29 Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexes; involved in RNA polymerase II transcription initiation and in chromatin modification; contains a YEATS domain peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification, DNA-templated transcription, initiation, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation nucleic acid binding transcription factor activity, hydrolase activity, transcription factor activity, protein binding, ATPase activity nucleus, chromosome 2123 BY4741 16_2 9 G 2 YPL127C HHO1 histone H1 Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination chromatin organization, regulation of organelle organization, DNA recombination, regulation of DNA metabolic process DNA binding nucleus, chromosome 2125 BY4741 16_2 9 G 3 YPL125W KAP120 LPH2 Karyopherin responsible for the nuclear import of Rpf1p; Rpf1p is a ribosome maturation factor protein targeting, nuclear transport protein transporter activity nucleus, membrane, endomembrane system, cytoplasm 2127 BY4741 16_2 9 G 4 YPL123C RNY1 ribonuclease T2 Vacuolar RNase of the T(2) family; relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; required for tRNA-specific translational pausing suring oxidative stress; promotes apoptosis under stress conditions and this function is independent of Rny1p catalytic activity RNA catabolic process, cell morphogenesis nuclease activity, hydrolase activity extracellular region, vacuole, cytoplasm 2129 BY4741 16_2 9 G 5 YPL121C MEI5 LPH6 Meiosis-specific protein involved in meiotic recombination; involved in DMC1-dependent meiotic recombination; forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p meiotic cell cycle, DNA repair, organelle fission, DNA recombination, cellular response to DNA damage stimulus molecular_function nucleus, chromosome 2131 BY4741 16_2 9 G 6 YPL120W VPS30 beclin 1|ATG6|VPT30|APG6 Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy, Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; C-terminus has novel globular fold essential for autophagy through the targeting of the PI3-kinase complex I to the pre-autophagosomal structure; ortholog of higher eukaryote gene Beclin 1; human BECN1 can complement yeast null mutant protein targeting, peroxisome organization, lipid metabolic process, membrane invagination, endosomal transport molecular_function vacuole, membrane, cytoplasm 2132 BY4741 16_2 9 G 7 YPL119C DBP1 putative DEAD-box ATP-dependent RNA helicase DBP1|LPH8 Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress; DBP1 has a paralog, DED1, that arose from the whole genome duplication translational initiation RNA binding, mRNA binding, helicase activity, hydrolase activity cytoplasm 2133 BY4741 16_2 9 G 8 YPL118W MRP51 mitochondrial 37S ribosomal protein MRP51 Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences translational initiation, mitochondrial translation, mitochondrion organization structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm 2134 BY4741 16_2 9 G 9 YPL116W HOS3 histone deacetylase Trichostatin A-insensitive homodimeric histone deacetylase (HDAC); specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats mitotic cell cycle, chromatin organization, histone modification, transcription from RNA polymerase II promoter hydrolase activity nucleus, cellular bud, membrane, site of polarized growth, cytoplasm 2136 BY4741 16_2 9 G 10 YPL115C BEM3 - Rho GTPase activating protein (RhoGAP); involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly cytoskeleton organization, signaling lipid binding, enzyme regulator activity, ion binding cell cortex, cellular bud, site of polarized growth, cytoplasm 2137 BY4741 16_2 9 G 11 YPL114W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index biological_process molecular_function cellular_component 2138 BY4741 16_2 9 G 12 YPL113C - glyoxylate reductase Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene biological_process oxidoreductase activity cellular_component 2139 BY4741 16_2 9 H 1 YPL112C PEX25 - Peripheral peroxisomal membrane peroxin; required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with Pex27p; PEX25 has a paralog, PEX27, that arose from the whole genome duplication protein targeting, transmembrane transport, peroxisome organization molecular_function peroxisome, membrane, cytoplasm 2140 BY4741 16_2 9 H 3 YPL111W CAR1 arginase|cargA|LPH15 Arginase, catabolizes arginine to ornithine and urea; expression responds to both induction by arginine and nitrogen catabolite repression; disruption decreases production of carcinogen ethyl carbamate during wine fermentation and also enhances freeze tolerance cellular amino acid metabolic process ion binding, hydrolase activity, enzyme regulator activity nucleus, site of polarized growth, cytoplasm 2141 BY4741 16_2 9 H 4 YPL110C GDE1 - Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes lipid metabolic process hydrolase activity ribosome, cytoplasm 2142 BY4741 16_2 9 H 5 YPL109C - - UbiB family protein; contains transmembrane domain and mitochondrial targeting sequence; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 2143 BY4741 16_2 9 H 6 YPL108W - - Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function cytoplasm 2144 BY4741 16_2 9 H 7 YPL107W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene biological_process molecular_function mitochondrion, cytoplasm 2145 BY4741 16_2 9 H 8 YPL106C SSE1 adenyl-nucleotide exchange factor SSE1|MSI3|LPG3 ATPase component of heat shock protein Hsp90 chaperone complex; plays a role in determining prion variants; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; deletion results in spindle elongation in S phase; SSE1 has a paralog, SSE2, that arose from the whole genome duplication protein folding ion binding, enzyme regulator activity cytoplasm 2146 BY4741 16_2 9 H 9 YPL105C SYH1 MYR1 Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication nucleus organization molecular_function membrane, ribosome, mitochondrion, cytoplasm 2147 BY4741 16_2 9 H 10 YPL104W MSD1 aspartate--tRNA ligase MSD1|LPG5 Mitochondrial aspartyl-tRNA synthetase; required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 2148 BY4741 16_2 9 H 11 YPL103C FMP30 N-acetylphosphatidylethanolamine-hydrolyzing phospholipase D Protein with a role in maintaining mitochondrial morphology; also involved in maintaining normal cardiolipin levels; mitochondrial inner membrane protein; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D lipid metabolic process hydrolase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 2149 BY4741 16_2 9 H 12 YPL101W ELP4 Elongator subunit ELP4|KTI9|TOT7|HAP1 Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity; null mutation is functionally complemented by human ELP4 tRNA processing, transcription from RNA polymerase II promoter, RNA modification RNA binding, ATPase activity, hydrolase activity cytoplasm 2150 BY4741 16_2 10 A 1 YPL102C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 biological_process molecular_function cellular_component 2151 BY4741 16_2 10 A 2 YPL100W ATG21 HSV1|MAI1 Phosphoinositide binding protein; required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein protein targeting, mitochondrion organization, vesicle organization, membrane invagination, protein lipidation lipid binding, ion binding endomembrane system, vacuole, membrane, cytoplasm 2152 BY4741 16_2 10 A 3 YPL099C INA17 AIM43|FMP14 F1F0 ATPase synthase peripheral stalk assembly factor; subunit of the matrix-exposed inner mitochondrial membrane localized INA complex (Ina22p-Ina17p) involved in assembly of the F1F0 peripheral stalk; co-purifies with Ina22p and ATP synthase subunits; null mutant displays elevated frequency of mitochondrial genome loss and has a respiratory growth defect mitochondrion organization, protein complex biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 2153 BY4741 16_2 10 A 4 YPL098C MGR2 - Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; provides quality control for insertion of membrane proteins by regulating their release into the inner membrane by the TIM23 complex; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) protein targeting, mitochondrion organization, transmembrane transport molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 2154 BY4741 16_2 10 A 5 YPL097W MSY1 tyrosine--tRNA ligase MSY1 Mitochondrial tyrosyl-tRNA synthetase mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 2155 BY4741 16_2 10 A 6 YPL096W PNG1 - Conserved peptide N-glycanase; required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p proteolysis involved in cellular protein catabolic process hydrolase activity nucleus, mitochondrion, cytoplasm 2156 BY4741 16_2 10 A 7 YPL095C EEB1 - Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication lipid metabolic process, monocarboxylic acid metabolic process transferase activity, hydrolase activity cellular_component 2157 BY4741 16_2 10 A 8 YPL092W SSU1 LPG16 Plasma membrane sulfite pump involved in sulfite metabolism; required for efficient sulfite efflux; major facilitator superfamily protein ion transport transmembrane transporter activity membrane, plasma membrane 2160 BY4741 16_2 10 A 9 YBR174C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective biological_process molecular_function cellular_component 3314 BY4741 02_4 10 A 11 YBR175W SWD3 SAF35|CPS30 Essential subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 peptidyl-amino acid modification, telomere organization, protein alkylation, chromatin organization, histone modification transferase activity, methyltransferase activity nucleus 3315 BY4741 02_4 10 A 12 YBR176W ECM31 3-methyl-2-oxobutanoate hydroxymethyltransferase Ketopantoate hydroxymethyltransferase; required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate cofactor metabolic process, vitamin metabolic process, monocarboxylic acid metabolic process transferase activity mitochondrion, cytoplasm 3316 BY4741 02_4 10 B 1 YBR177C EHT1 - Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication monocarboxylic acid metabolic process, lipid metabolic process transferase activity, hydrolase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 3317 BY4741 02_4 10 B 2 YBR178W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C biological_process molecular_function cellular_component 3318 BY4741 02_4 10 B 3 YBR179C FZO1 mitofusin Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system mitochondrion organization, organelle fusion, membrane fusion GTPase activity, hydrolase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 3319 BY4741 02_4 10 B 4 YBR180W DTR1 - Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; sequence similarity to QDR1 and QDR3, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expressed during sporulation meiotic cell cycle, sporulation, transmembrane transport, cell wall organization or biogenesis transmembrane transporter activity membrane 3320 BY4741 02_4 10 B 5 YBR181C RPS6B ribosomal 40S subunit protein S6B|S6e|RPS102|RPS101|LPG18 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 3321 BY4741 02_4 10 B 6 YBR182C SMP1 - MADS-box transcription factor involved in osmotic stress response; SMP1 has a paralog, RLM1, that arose from the whole genome duplication transcription from RNA polymerase II promoter, response to osmotic stress nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 3322 BY4741 02_4 10 B 7 YBR183W YPC1 phytoceramidase Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication lipid metabolic process transferase activity, hydrolase activity, hydrolase activity, acting on glycosyl bonds cell cortex, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 3323 BY4741 02_4 10 B 8 YBR184W - - Putative protein of unknown function; YBR184W is not an essential gene biological_process molecular_function cellular_component 3324 BY4741 02_4 10 B 9 YBR185C MBA1 - Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other mitochondrion, membrane, mitochondrial envelope, cytoplasm 3325 BY4741 02_4 10 B 10 YBR186W PCH2 - Hexameric ring ATPase that remodels chromosome axis protein Hop1p; nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in rDNA; required for meiotic double-stranded break formation meiotic cell cycle, organelle fission, regulation of organelle organization, DNA recombination, regulation of cell cycle hydrolase activity, ATPase activity nucleus, nucleolus 3326 BY4741 02_4 10 B 11 YBR187W GDT1 putative ribosome biosynthesis protein GDT1 Protein of unknown function involved in calcium homeostasis; localizes to the cis- and medial-Golgi apparatus; GFP-fusion protein localizes to the vacuole; TMEM165, a human gene which causes Congenital Disorders of Glycosylation is orthologous and functionally complements the null allele; expression pattern and physical interactions suggest a possible role in ribosome biogenesis; expression reduced in a gcr1 null mutant cellular ion homeostasis molecular_function membrane, endomembrane system, vacuole, cytoplasm, Golgi apparatus 3327 BY4741 02_4 10 B 12 YBR188C NTC20 - Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs RNA splicing, mRNA processing other nucleus 3328 BY4741 02_4 10 C 1 YBR194W AIM4 SOY1 Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress biological_process molecular_function cytoplasm 3334 BY4741 02_4 10 C 2 YBR195C MSI1 CAC3 Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 affects multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of DNA damage checkpoint after DNA repair; chromatin dynamics during transcription; and repression of divergent noncoding transcription; Msi1p localizes to nucleus and cytoplasm and independently regulates the RAS/cAMP pathway via sequestration of Npr1p kinase chromatin organization, protein complex biogenesis histone binding nucleus, cytoplasm 3335 BY4741 02_4 10 C 3 YBR197C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication biological_process molecular_function nucleus, cytoplasm 3337 BY4741 02_4 10 C 4 YBR199W KTR4 putative mannosyltransferase Glycosyltransferase involved in protein glycosylation; transfers GDP-mannose to methyl-alpha-mannoside in vitro; member of the KRE2/MNT1 mannosyltransferase family of type II membrane proteins with a short cytoplasmic N-terminus, a membrane-spanning region and a highly conserved catalytic lumenal domain protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups, transferase activity endomembrane system, cytoplasm, Golgi apparatus 3339 BY4741 02_4 10 C 5 YBR200W BEM1 phosphatidylinositol-3-phosphate-binding protein BEM1|SRO1 Protein containing SH3-domains; involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p response to chemical, cell morphogenesis, conjugation lipid binding, structural molecule activity, ion binding mitochondrion, cellular bud, site of polarized growth, cytoplasm 3340 BY4741 02_4 10 C 6 YBR201W DER1 - ER membrane protein that promotes export of misfolded polypeptides; required for ER-associated protein degradation of misfolded or unassembled proteins; initiates export of aberrant polypeptides from ER lumen by threading them into the ER membrane and routing them to Hrd1p for ubiquitylation; N- and C- termini protrude into the cytoplasm; similar to Dfm1p; homolog of mammalian derlin-1 response to chemical, proteolysis involved in cellular protein catabolic process other membrane, endomembrane system, endoplasmic reticulum, cytoplasm 3341 BY4741 02_4 10 C 7 YBR203W COS111 - Protein required for antifungal drug ciclopirox olamine resistance; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies signaling molecular_function mitochondrion, cytoplasm 3343 BY4741 02_4 10 C 8 YBR204C LDH1 - Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) other hydrolase activity other 3344 BY4741 02_4 10 C 9 YBR205W KTR3 mannosyltransferase KTR3 Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress carbohydrate metabolic process, protein glycosylation, oligosaccharide metabolic process transferase activity, transferring glycosyl groups, transferase activity nucleus, vacuole, membrane, cytoplasm 3345 BY4741 02_4 10 C 10 YBR206W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 biological_process molecular_function cellular_component 3346 BY4741 02_4 10 C 11 YBR207W FTH1 - Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress transmembrane transport, ion transport, endocytosis transmembrane transporter activity membrane, vacuole, cytoplasm 3347 BY4741 02_4 10 C 12 YBR208C DUR1,2 bifunctional urea carboxylase/allophanate hydrolase|DUR80 Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress other ligase activity, hydrolase activity cytoplasm 3348 BY4741 02_4 10 D 1 YBR209W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YBR209W is not an essential gene biological_process molecular_function cellular_component 3349 BY4741 02_4 "Hypothetical protein -- growth on -met, no growth on -lys, no growth on drop-in media" 10 D 2 YBR210W ERV15 - Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication mitotic cell cycle, cell budding, cytokinesis, Golgi vesicle transport molecular_function endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, cytoplasm 3350 BY4741 02_4 10 D 3 YBR212W NGR1 RBP1 RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase regulation of translation, mitochondrion organization RNA binding, mRNA binding cytoplasm 3352 BY4741 02_4 10 D 4 YBR213W MET8 bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase MET8 Bifunctional dehydrogenase and ferrochelatase; involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis cofactor metabolic process lyase activity, oxidoreductase activity cellular_component 3353 BY4741 02_4 10 D 5 YBR214W SDS24 - Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication endocytosis molecular_function cytoplasm 3354 BY4741 02_4 10 D 6 YBR215W HPC2 - Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes mitotic cell cycle, chromatin organization, transcription from RNA polymerase II promoter, protein complex biogenesis, DNA-templated transcription, elongation chromatin binding, DNA binding nucleus 3355 BY4741 02_4 10 D 7 YBR216C YBP1 - Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication response to chemical, response to oxidative stress molecular_function cytoplasm 3356 BY4741 02_4 10 D 8 YBR217W ATG12 APG12 Ubiquitin-like modifier involved in autophagy and the Cvt pathway; conserved; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg5p-Atg12p cojugate also forms a complex with Atg16p; the Atg5-Atg12/Atg16 complex binds to membranes and is essential for autophagosome formation vacuole organization, protein targeting, membrane invagination, mitochondrion organization, organelle assembly, protein lipidation transferase activity, enzyme regulator activity cytoplasm 3357 BY4741 02_4 10 D 9 YBR218C PYC2 pyruvate carboxylase 2 Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication carbohydrate metabolic process ligase activity cytoplasm 3358 BY4741 02_4 10 D 10 YBR219C - - Putative protein of unknown function; YBR219C is not an essential gene biological_process molecular_function membrane 3359 BY4741 02_4 10 D 11 YBR220C - - Putative protein of unknown function; YBR220C is not an essential gene biological_process molecular_function membrane 3360 BY4741 02_4 10 D 12 YBR221C PDB1 pyruvate dehydrogenase (acetyl-transferring) subunit E1 beta E1 beta subunit of the pyruvate dehydrogenase (PDH) complex; PDH is an evolutionarily conserved multi-protein complex found in mitochondria cofactor metabolic process, monocarboxylic acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 3361 BY4741 02_4 10 E 1 YBR222C PCS60 FAT2 Oxalyl-CoA synthetase; capable of catalyzing conversion of oxalate to oxalyl-CoA; catalyzes first step in pathway of oxalate degradation that functions to protect yeast from inhibitory effects of oxalate; peroxisomal protein that binds mRNA; localizes to both peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid; similar to E. coli long chain acyl-CoA synthetase other RNA binding, mRNA binding, ligase activity peroxisome, membrane, cytoplasm 3362 BY4741 02_4 10 E 2 YBR223C TDP1 tyrosyl-DNA phosphodiesterase 1 Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCAN1 DNA repair, cellular response to DNA damage stimulus hydrolase activity nucleus 3363 BY4741 02_4 10 E 3 YBR224W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 biological_process molecular_function membrane 3364 BY4741 02_4 10 E 4 YBR225W - - Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components biological_process molecular_function cellular_component 3365 BY4741 02_4 10 E 5 YBR226C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W biological_process molecular_function cellular_component 3366 BY4741 02_4 10 E 6 YBR227C MCX1 - Non-proteolytic ATPase of the AAA family; stimulates incorporation of the pyridoxal phosphate cofactor into Hem1p (5-aminolevulinic acid synthase); localized to the mitochondrial matrix; ortholog of vertebrate CLPX, which promotes erythropoiesis regulation of protein modification process, protein folding ATPase activity, hydrolase activity mitochondrion, cytoplasm 3367 BY4741 02_4 10 E 7 YBR228W SLX1 endonuclease Endonuclease involved in DNA recombination and repair; subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p DNA replication nuclease activity, hydrolase activity nucleus, chromosome 3368 BY4741 02_4 10 E 8 YBR229C ROT2 glucan 1,3-alpha-glucosidase ROT2|GLS2 Glucosidase II catalytic subunit; required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations carbohydrate metabolic process, cell wall organization or biogenesis hydrolase activity, hydrolase activity, acting on glycosyl bonds endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm 3369 BY4741 02_4 10 E 9 YBR230C OM14 - Mitochondrial outer membrane receptor for cytosolic ribosomes; integral protein of the outer membrane that interacts with the nascent chain-associated complex (NAC) bound to ribosomes, contributing to co-translational mitochondrial import; interacts with porin (Por1p) and Om45p; abundance is decreased in cells grown in glucose relative to other carbon sources protein targeting, mitochondrion organization other membrane, mitochondrial envelope, mitochondrion, cytoplasm 3370 BY4741 02_4 10 E 10 YBR231C SWC5 AOR1 Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia chromatin organization molecular_function nucleus, chromosome, cytoplasm 3371 BY4741 02_4 10 E 11 YBR233W PBP2 HEK1 RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress telomere organization RNA binding, mRNA binding nucleus, cytoplasm 3373 BY4741 02_4 10 E 12 YBR235W VHC1 - Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs); similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family transmembrane transport transmembrane transporter activity membrane, vacuole, cytoplasm 3375 BY4741 02_4 10 F 1 YBR238C - - Mitochondrial membrane protein; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span; YBR238C has a paralog, RMD9, that arose from the whole genome duplication generation of precursor metabolites and energy, cellular respiration RNA binding, mRNA binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 3378 BY4741 02_4 10 F 2 YBR239C ERT1 - Transcriptional regulator; involved in regulation of gluconeogenesis and fermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition; member of the zinc cluster family of proteins, similar to Rds2p carbohydrate metabolic process, response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 3379 BY4741 02_4 10 F 3 YBR240C THI2 phoF|PHO6 Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type vitamin metabolic process, transcription from RNA polymerase II promoter DNA binding nucleus 3380 BY4741 02_4 10 F 4 YBR241C - - Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene; YBR241C has a paralog, VPS73, that arose from the whole genome duplication transmembrane transport transmembrane transporter activity membrane, vacuole, cytoplasm 3381 BY4741 02_4 10 F 5 YBR242W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene; YBR242W has a paralog, YGL101W, that arose from the whole genome duplication biological_process molecular_function nucleus, cytoplasm 3382 BY4741 02_4 10 F 6 YBR244W GPX2 glutathione peroxidase GPX2|AMI1 Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress response to chemical, response to oxidative stress oxidoreductase activity nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm 3384 BY4741 02_4 10 F 7 YBR245C ISW1 chromatin-remodeling ATPase ISW1|SGN2 ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; with Ioc3p forms Isw1a complex involved in repression of transcription initiation; with Ioc2p and Ioc4p forms Isw1b complex involved in regulation of transcription elongation; Isw1b recruited to ORFs by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions; Isw1p import into nucleus depends on C-terminal bipartite nuclear targeting signal KRIR X19 KKAK transcription from RNA polymerase I promoter, chromatin organization, regulation of organelle organization, protein complex biogenesis, DNA-templated transcription, termination, DNA-templated transcription, initiation, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation chromatin binding, hydrolase activity, DNA binding, ATPase activity nucleus, chromosome, nucleolus 3385 BY4741 02_4 10 F 8 YBR246W RRT2 DPH7|diphthamide synthase|ERE1 Methylesterase performing penultimate step of diphthamide biosynthesis; hydrolyzes methylated diphthine to produce diphthine and allows Dph6-catalyzed amidation reaction to occur; deletion leads to resistance to sordarin and accumulation of methylatediphthine; WD40 domain-containing protein; involved in endosomal recycling; forms complex with Rtt10p that functions in retromer-mediated pathway for recycling internalized cell-surface proteins peptidyl-amino acid modification, endosomal transport hydrolase activity endomembrane system, cytoplasm 3386 BY4741 02_4 10 F 9 YBR248C HIS7 imidazoleglycerol-phosphate synthase Imidazole glycerol phosphate synthase; glutamine amidotransferase:cyclase that catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor cellular amino acid metabolic process transferase activity, transferring glycosyl groups, transferase activity, hydrolase activity other 3388 BY4741 02_4 10 F 10 YBR249C ARO4 3-deoxy-7-phosphoheptulonate synthase ARO4 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress other transferase activity nucleus, cytoplasm 3389 BY4741 02_4 10 F 11 YBR250W SPO23 - Protein of unknown function; associates with meiosis-specific protein Spo1p meiotic cell cycle, organelle fission molecular_function cellular_component 3390 BY4741 02_4 10 F 12 YBR251W MRPS5 mitochondrial 37S ribosomal protein MRPS5 Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 3391 BY4741 02_4 10 G 1 YBR255W MTC4 - Protein of unknown function; required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 biological_process molecular_function cytoplasm 3395 BY4741 02_4 10 G 2 YBR258C SHG1 CPS15 Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres peptidyl-amino acid modification, protein alkylation, chromatin organization, histone modification transferase activity, methyltransferase activity nucleus 3398 BY4741 02_4 10 G 3 YBR259W - - Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress biological_process molecular_function cellular_component 3399 BY4741 02_4 10 G 4 YBR260C RGD1 - GTPase-activating protein (RhoGAP) for Rho3p and Rho4p; possibly involved in control of actin cytoskeleton organization signaling, response to osmotic stress lipid binding, enzyme regulator activity, ion binding cell cortex, cytoskeleton, cellular bud, membrane, site of polarized growth, cytoplasm 3400 BY4741 02_4 10 G 5 YBR261C TAE1 N-terminal protein methyltransferase|NTM1 AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm peptidyl-amino acid modification, cytoplasmic translation, protein alkylation transferase activity, methyltransferase activity cytoplasm 3401 BY4741 02_4 10 G 6 YBR262C MIC12 AIM5|MCS12|FMP51 Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; forms a subcomplex with Mic10p and Mic27p whose assembly and stability requires cardiolipin mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 3402 BY4741 02_4 10 G 7 YBR263W SHM1 glycine hydroxymethyltransferase SHM1|TMP3|SHMT1 Mitochondrial serine hydroxymethyltransferase; converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine cellular amino acid metabolic process transferase activity mitochondrion, cytoplasm 3403 BY4741 02_4 10 G 8 YBR264C YPT10 Rab family GTPase YPT10 Rab family GTP-binding protein; contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles other GTPase activity, hydrolase activity cytoplasm 3404 BY4741 02_4 10 G 9 YBR266C SLM6 - Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase endocytosis molecular_function cellular_component 3406 BY4741 02_4 10 G 10 YBR267W REI1 - Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network mitotic cell cycle, cell budding, ribosomal large subunit biogenesis, nuclear transport DNA binding ribosome, cytoplasm 3407 BY4741 02_4 Incorrect -- PCR mating type A/Alpha 10 G 11 YBR268W MRPL37 mitochondrial 54S ribosomal protein YmL37|YmL37 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 3408 BY4741 02_4 10 G 12 YDR049W VMS1 - Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans response to chemical, proteolysis involved in cellular protein catabolic process peptidase activity, hydrolase activity endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 3985 BY4741 04_4 10 H 1 YDR050C TPI1 triose-phosphate isomerase TPI1 Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human homolog TPI1 causes a rare autosomal disease; human TPI1 can complement yeast null mutant carbohydrate metabolic process, cofactor metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, nucleobase-containing small molecule metabolic process isomerase activity mitochondrion, membrane, cytoplasm, plasma membrane 3986 BY4741 04_4 10 H 3 YDR051C DET1 acid phosphatase DET1 Acid phosphatase; involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel lipid transport phosphatase activity, hydrolase activity nucleus, cytoplasm 3987 BY4741 04_4 10 H 4 YDR055W PST1 HPF2 Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1; PST1 has a paralog, ECM33, that arose from the whole genome duplication cell wall organization or biogenesis molecular_function cell wall, membrane, plasma membrane 3991 BY4741 04_4 10 H 5 YDR056C EMC10 - Putative protein of unknown function; subunit of evolutionarily conserved EMC (Endoplasmic Reticulum Membrane Complex) implicated in ERAD (ER-associated degradation) and proper assembly of multi-pass transmembrane (TM) proteins; EMC acts in yeast as an ER-mitochondria tether that interacts with outer membrane protein Tom5p of TOM (Translocase of the Mitochondrial Outer Membrane) complex; YDR056C is not an essential protein biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm 3992 BY4741 04_4 10 H 6 YDR057W YOS9 - ER quality-control lectin; integral subunit of the HRD ligase; participates in efficient ER retention of misfolded proteins by recognizing them and delivering them to Hrd1p; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family response to chemical, proteolysis involved in cellular protein catabolic process, signaling other endomembrane system, membrane, endoplasmic reticulum, cytoplasm 3993 BY4741 04_4 10 H 7 YDR059C UBC5 E2 ubiquitin-conjugating protein UBC5 Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication response to heat, protein modification by small protein conjugation or removal transferase activity other 3994 BY4741 04_4 10 H 8 YDR061W - - Protein with similarity to ABC transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance biological_process molecular_function mitochondrion, cytoplasm 3996 BY4741 04_4 10 H 9 YDR063W AIM7 GMF1|GMF Protein that interacts with Arp2/3 complex; interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss cytoskeleton organization, regulation of organelle organization, protein complex biogenesis molecular_function nucleus, cell cortex, cytoskeleton, cytoplasm 3998 BY4741 04_4 10 H 10 YDR065W RRG1 - Protein of unknown function; required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies cellular ion homeostasis, mitochondrion organization molecular_function mitochondrion, cytoplasm 4000 BY4741 04_4 10 H 11 YDR066C RTR2 putative protein-serine/threonine phosphatase Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication biological_process molecular_function nucleus, cytoplasm 4001 BY4741 04_4 10 H 12 YDR067C OCA6 - Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT biological_process molecular_function cytoplasm 4002 BY4741 04_4 11 A 1 YDR068W DOS2 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm 4003 BY4741 04_4 11 A 2 YDR069C DOA4 ubiquitin-specific protease DOA4|UBP4|SSV7|NPI2|MUT4|DOS1 Ubiquitin hydrolase; deubiquitinates intralumenal vesicle (ILVs) cargo proteins; required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins destined for the vacuole; DOA4 has a paralog, UBP5, that arose from the whole genome duplication DNA replication, vesicle organization, proteolysis involved in cellular protein catabolic process, regulation of DNA metabolic process, endocytosis peptidase activity, hydrolase activity endomembrane system, mitochondrion, cytoplasm 4004 BY4741 04_4 11 A 3 YDR070C FMP16 - Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress biological_process molecular_function mitochondrion, cytoplasm 4005 BY4741 04_4 11 A 4 YDR072C IPT1 MIC2|inositolphosphotransferase|KTI6|SYR4 Inositolphosphotransferase; involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin lipid metabolic process transferase activity membrane 4007 BY4741 04_4 11 A 5 YDR073W SNF11 - Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions transcription from RNA polymerase II promoter ATPase activity, hydrolase activity nucleus, cytoplasm 4008 BY4741 04_4 11 A 6 YDR075W PPH3 phosphoprotein phosphatase PP4 catalytic subunit PPH3 Catalytic subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form active complex, Psy4p may provide substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes meiotic cell cycle, response to chemical, organelle fission, protein dephosphorylation, DNA recombination, DNA repair, regulation of organelle organization, cellular response to DNA damage stimulus, regulation of DNA metabolic process, regulation of cell cycle, signaling phosphatase activity, hydrolase activity nucleus, chromosome, cytoplasm 4010 BY4741 04_4 11 A 7 YDR076W RAD55 putative DNA-dependent ATPase RAD55 Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p meiotic cell cycle, DNA repair, organelle fission, DNA recombination, cellular response to DNA damage stimulus ATPase activity, hydrolase activity nucleus 4011 BY4741 04_4 11 A 8 YDR077W SED1 - Major stress-induced structural GPI-cell wall glycoprotein; associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites; SED1 has a paralog, SPI1, that arose from the whole genome duplication mitochondrion organization, cell wall organization or biogenesis structural molecule activity cell wall, ribosome, mitochondrion, cytoplasm 4012 BY4741 04_4 11 A 9 YDR078C SHU2 - Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Csm2, Shu1, and promotes error-free DNA repair, Shu complex mediates inhibition of Srs2p function; promotes formation of Rad51p filaments; important for both mitotic and meiotic homologous recombination, and contains a conserved SWIM domain that is necessary for both DNA repair, DNA recombination, cellular response to DNA damage stimulus molecular_function chromosome 4013 BY4741 04_4 11 A 10 YDR079W PET100 - Chaperone that facilitates the assembly of cytochrome c oxidase; integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme mitochondrion organization, protein complex biogenesis unfolded protein binding membrane, mitochondrial envelope, mitochondrion, cytoplasm 4014 BY4741 04_4 11 A 12 YDR080W VPS41 VPL20|VAM2|SVL2|FET2|CVT8 Subunit of the HOPS endocytic tethering complex; vacuole membrane protein that functions as a Rab GTPase effector, interacting specifically with the GTP-bound conformation of Ypt7p, facilitating tethering, docking and promoting membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; Yck3p-mediated phosphorylation regulates the organization of vacuolar fusion sites vacuole organization, regulation of transport, organelle fusion, protein maturation, protein targeting, regulation of organelle organization, vesicle organization, protein complex biogenesis, membrane fusion, membrane invagination enzyme binding, ion binding, lipid binding vacuole, endomembrane system, membrane, cytoplasm 4015 BY4741 04_4 11 B 1 YDR083W RRP8 25S rRNA (adenine645-N1)-methyltransferase Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 rRNA processing, ribosomal large subunit biogenesis, RNA modification transferase activity, methyltransferase activity nucleus, nucleolus 4018 BY4741 04_4 11 B 2 YDR084C TVP23 - Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern other molecular_function membrane, endomembrane system, cytoplasm, Golgi apparatus 4019 BY4741 04_4 11 B 3 YDR085C AFR1 - Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication response to chemical, cell morphogenesis, conjugation, signaling signal transducer activity other 4020 BY4741 04_4 11 B 4 YDR089W - - Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress other molecular_function membrane, vacuole, cytoplasm 4024 BY4741 04_4 11 B 5 YDR090C - - Putative protein of unknown function biological_process molecular_function membrane 4025 BY4741 04_4 11 B 6 YDR092W UBC13 E2 ubiquitin-conjugating protein UBC13 E2 ubiquitin-conjugating enzyme; involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus DNA repair, protein modification by small protein conjugation or removal, cellular response to DNA damage stimulus, protein complex biogenesis transferase activity nucleus, cytoplasm 4027 BY4741 04_4 11 B 7 YDR093W DNF2 aminophospholipid-translocating P4-type ATPase DNF2 Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF2 has a paralog, DNF1, that arose from the whole genome duplication response to chemical, ion transport, lipid transport, conjugation, endocytosis hydrolase activity, ATPase activity membrane, site of polarized growth, plasma membrane 4028 BY4741 04_4 11 B 8 YDR094W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2/YDR093W biological_process molecular_function cellular_component 4029 BY4741 04_4 11 B 9 YDR095C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4030 BY4741 04_4 11 B 10 YDR096W GIS1 histone demethylase GIS1 Histone demethylase and transcription factor; regulates genes during nutrient limitation; activity modulated by proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminus that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; relocalizes to the cytosol in response to hypoxia; GIS1 has a paralog, RPH1, that arose from the whole genome duplication chromatin organization, histone modification, lipid metabolic process, response to starvation, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, oxidoreductase activity, DNA binding nucleus, mitochondrion, cytoplasm 4031 BY4741 04_4 11 B 11 YDR097C MSH6 PMS6|mismatch repair ATPase MSH6|PMS3 Protein required for mismatch repair in mitosis and meiosis; forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p meiotic cell cycle, DNA repair, organelle fission, cellular response to DNA damage stimulus hydrolase activity, DNA binding, ion binding, ATPase activity nucleus, cytoplasm 4032 BY4741 04_4 11 B 12 YDR098C GRX3 monothiol glutaredoxin GRX3 Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; with Grx4p, binds to Aft1p in iron-replete conditions, promoting its dissociation from promoters; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36; GRX3 has a paralog, GRX4, that arose from the whole genome duplication response to chemical, cytoskeleton organization, response to oxidative stress, cellular ion homeostasis oxidoreductase activity, transcription factor binding nucleus, cytoplasm 4033 BY4741 04_4 11 C 1 YDR099W BMH2 14-3-3 family protein BMH2|SCD3 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; abundance relative to Bmh1p increases during sporulation mitotic cell cycle, meiotic cell cycle, DNA replication, generation of precursor metabolites and energy, cell wall organization or biogenesis, carbohydrate metabolic process, sporulation, cellular response to DNA damage stimulus, regulation of protein modification process, protein modification by small protein conjugation or removal, pseudohyphal growth, signaling ion binding, DNA binding nucleus, plasma membrane, membrane, cytoplasm 4034 BY4741 04_4 11 C 2 YDR100W TVP15 - Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p other molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 4035 BY4741 04_4 11 C 3 YDR101C ARX1 - Nuclear export factor for the ribosomal pre-60S subunit; shuttling factor which directly binds FG rich nucleoporins and facilities translocation through the nuclear pore complex; interacts directly with Alb1p; responsible for Tif6p recycling defects in the absence of Rei1; associated with the ribosomal export complex ribosomal subunit export from nucleus, nuclear transport molecular_function nucleus, nucleolus, ribosome, cytoplasm 4036 BY4741 04_4 11 C 4 YDR102C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; homozygous diploid deletion strain exhibits high budding index biological_process molecular_function cellular_component 4037 BY4741 04_4 11 C 5 YDR103W STE5 NUL3|HMD3 Pheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation response to chemical, protein phosphorylation, invasive growth in response to glucose limitation, transposition, regulation of protein modification process, conjugation, signaling lipid binding, enzyme binding, structural molecule activity, ion binding nucleus, membrane, site of polarized growth, cytoplasm, plasma membrane 4038 BY4741 04_4 11 C 6 YDR104C SPO71 - Meiosis-specific protein required for prospore membrane morphogenesis; localizes to the prospore membrane (PSM) during sporulation; required for PSM elongation and closure; genetically antagonistic to SPO1; recruits Vps13p to the PSM during sporulation; interacts and functions cooperatively with Spo73p; mutants have defects in the PSM, aberrant spore wall formation and reduced PtdIns-phosphate pools in the PSM; contains two PH domains meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function membrane, plasma membrane 4039 BY4741 04_4 11 C 7 YDR105C TMS1 - Vacuolar membrane protein of unknown function; is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance biological_process molecular_function membrane, vacuole, cytoplasm 4040 BY4741 04_4 11 C 8 YDR107C TMN2 - Protein with a role in cellular adhesion and filamentous growth; similar to Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments; TMN2 has a paralog, EMP70, that arose from the whole genome duplication cellular ion homeostasis, invasive growth in response to glucose limitation, pseudohyphal growth molecular_function membrane, vacuole, cytoplasm 4041 BY4741 04_4 11 C 9 YDR108W TRS85 GSG1|MUM1 Component of transport protein particle (TRAPP) complex III; TRAPPIII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating endosome-Golgi traffic and required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role vacuole organization, meiotic cell cycle, protein targeting, organelle fission, organelle assembly, vesicle organization, peroxisome organization, Golgi vesicle transport, membrane invagination molecular_function endomembrane system, Golgi apparatus, cytoplasm 4042 BY4741 04_4 11 C 10 YDR109C - putative phosphotransferase Putative kinase biological_process molecular_function cellular_component 4043 BY4741 04_4 11 C 11 YDR110W FOB1 replication fork barrier binding protein FOB1|HRM1 Nucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to replication fork barrier (RFB), and rDNA repeat segregation; related to retroviral integrases chromatin organization, DNA replication, chromosome segregation, DNA recombination, regulation of DNA metabolic process, nucleus organization DNA binding nucleus, chromosome, nucleolus 4044 BY4741 04_4 11 C 12 YDR111C ALT2 alanine transaminase ALT2 Catalytically inactive alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p; ALT2 has a paralog, ALT1, that arose from the whole genome duplication biological_process transferase activity nucleus, cytoplasm 4045 BY4741 04_4 11 D 1 YDR112W IRC2 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ALT2/YDR111C; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function cellular_component 4046 BY4741 04_4 11 D 2 YDR114C - - Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium biological_process molecular_function cellular_component 4048 BY4741 04_4 11 D 3 YDR115W - putative mitochondrial 54S ribosomal protein Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress mitochondrial translation, mitochondrion organization structural constituent of ribosome, structural molecule activity nucleus, ribosome, mitochondrion, cytoplasm 4049 BY4741 04_4 11 D 4 YDR116C MRPL1 mitochondrial 54S ribosomal protein MRPL1 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4050 BY4741 04_4 11 D 5 YDR117C TMA64 RBF64 Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity rRNA processing RNA binding ribosome, cytoplasm 4051 BY4741 04_4 11 D 6 YDR119W VBA4 - Protein of unknown function; proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene biological_process molecular_function membrane, vacuole, cytoplasm 4053 BY4741 04_4 11 D 7 YDR120C TRM1 tRNA (guanine26-N2)-dimethyltransferase tRNA methyltransferase; two forms of protein are made by alternative translation starts; localizes to both nucleus and mitochondrion to produce modified base N2,N2-dimethylguanosine in tRNAs in both compartments; nuclear Trm1p is evenly distributed around inner nuclear membrane in WT, but mislocalizes as puncta near ER-nucleus junctions in spindle pole body (SPB) mutants; both Trm1p inner nuclear membrane targeting and maintenance depend upon SPB tRNA processing, RNA modification transferase activity, methyltransferase activity nucleus, endomembrane system, mitochondrion, membrane, cytoplasm 4054 BY4741 04_4 11 D 8 YDR121W DPB4 - Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization DNA repair, chromatin organization, DNA replication, cellular response to DNA damage stimulus chromatin binding, transferase activity, nucleotidyltransferase activity, DNA binding nucleus, chromosome 4055 BY4741 04_4 11 D 9 YDR122W KIN1 serine/threonine protein kinase KIN1 Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; KIN1 has a paralog, KIN2, that arose from the whole genome duplication protein phosphorylation, exocytosis kinase activity, transferase activity membrane, plasma membrane 4056 BY4741 04_4 11 D 10 YDR123C INO2 SCS1|DIE1 Transcription factor; component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion; involved in diauxic shift lipid metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity nucleus 4057 BY4741 04_4 11 D 11 YDR124W - - Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor biological_process molecular_function cellular_component 4058 BY4741 04_4 11 D 12 YDR125C ECM18 alpha/beta hydrolase family protein Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication biological_process molecular_function mitochondrion, cytoplasm 4059 BY4741 04_4 11 E 1 YDR126W SWF1 palmitoyltransferase SWF1|PSL10 Palmitoyltransferase that acts on transmembrane proteins; including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion vacuole organization, meiotic cell cycle, protein acylation, cytoskeleton organization, regulation of transport, organelle fusion, cell wall organization or biogenesis, protein lipidation, sporulation, membrane fusion, exocytosis transferase activity cell cortex, cytoskeleton, endomembrane system, membrane, cytoplasm 4060 BY4741 04_4 11 E 2 YDR127W ARO1 pentafunctional protein ARO1p Pentafunctional arom protein; catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids other lyase activity, kinase activity, transferase activity, oxidoreductase activity cytoplasm 4061 BY4741 04_4 11 E 3 YDR128W MTC5 SEA3 Subunit of SEACAT, a subcomplex of the SEA complex; Mtc1p, along with Rtc1p and Sea4p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; relative distribution to the vacuolar membrane decreases upon DNA replication stress signaling molecular_function membrane, vacuole, cytoplasm 4062 BY4741 04_4 11 E 4 YDR129C SAC6 fimbrin|ABP67 Fimbrin, actin-bundling protein; cooperates with calponin/transgelin Scp1p in organization and maintenance of actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress; human homologs PLS3 and LCP1 implicated in spinocerebellar ataxia type 2 (SCA2) can each complement yeast null mutant cytoskeleton organization protein binding, bridging, cytoskeletal protein binding cell cortex, cytoskeleton, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane 4063 BY4741 04_4 11 E 5 YDR130C FIN1 - Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress mitotic cell cycle, organelle fission, cytoskeleton organization, proteolysis involved in cellular protein catabolic process, regulation of organelle organization, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, regulation of cell cycle cytoskeletal protein binding, structural molecule activity nucleus, cytoskeleton, chromosome, microtubule organizing center, cytoplasm 4064 BY4741 04_4 11 E 6 YDR131C - - F-box protein subunit of SCF ubiquitin ligase complex; substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex proteolysis involved in cellular protein catabolic process protein binding, bridging, transferase activity other 4065 BY4741 04_4 11 E 7 YDR132C - - Protein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication biological_process molecular_function nucleus, mitochondrion, cytoplasm 4066 BY4741 04_4 11 E 8 YDR133C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps YDR134C biological_process molecular_function cellular_component 4067 BY4741 04_4 11 E 9 YDR134C cellular_component cellular_component cellular_component - - - 4068 BY4741 04_4 11 E 10 YDR135C YCF1 ATP-binding cassette glutathione S-conjugate transporter YCF1 Vacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR vacuole organization, response to chemical, transmembrane transport, ion transport, organelle fusion, membrane fusion hydrolase activity, transmembrane transporter activity, ATPase activity vacuole, membrane, cytoplasm 4069 BY4741 04_4 11 E 11 YDR136C VPS61 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect protein targeting molecular_function cellular_component 4070 BY4741 04_4 11 E 12 YDR137W RGP1 - Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p); this complex catalyzes nucleotide exchange on Ypt6p endosomal transport guanyl-nucleotide exchange factor activity membrane, endomembrane system, cytoplasm, Golgi apparatus 4071 BY4741 04_4 11 F 1 YPR021C AGC1 amino acid transporter AGC1 Mitochondrial amino acid transporter; acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis; human homolog SLC25A13 complements yeast null mutant ion transport, amino acid transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 7400 BY4741 00_18 "Hyperrecombination protein related to Top 1 p -- growth on -met, growth on -lys, mates poorly" 11 F 2 YDR139C RUB1 NEDD8 family protein RUB1 Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) protein modification by small protein conjugation or removal other cytoplasm 4073 BY4741 04_4 11 F 3 YDR140W MTQ2 - S-adenosylmethionine-dependent methyltransferase; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC; member of the seven beta-strand family regulation of translation, protein alkylation transferase activity, methyltransferase activity nucleus, cytoplasm 4074 BY4741 04_4 11 F 4 YDR142C PEX7 PEB1|PAS7 Peroxisomal signal receptor for peroxisomal matrix proteins; recognizes the N-terminal nonapeptide signal (PTS2); WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) protein targeting, transmembrane transport, peroxisome organization other peroxisome, cytoplasm 4076 BY4741 04_4 11 F 5 YDR143C SAN1 ubiquitin-protein ligase SAN1 Ubiquitin-protein ligase; involved in proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition; prefers a window of exposed hydrophobicity that causes a particular level of protein insolubility, suggesting that San1p evolved to target highly aggregation-prone proteins response to chemical, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process transferase activity nucleus, cytoplasm 4077 BY4741 04_4 11 F 6 YDR144C MKC7 YPS2 GPI-anchored aspartyl protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p; MKC7 has a paralog, YPS1, that arose from the whole genome duplication cell wall organization or biogenesis, signaling peptidase activity, hydrolase activity cell wall 4078 BY4741 04_4 11 F 7 YDR146C SWI5 DNA-binding transcription factor SWI5 Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication mitotic cell cycle, organelle fission, regulation of DNA metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding nucleus, cytoplasm 4080 BY4741 04_4 "Transcription factor -- growth on -met, growth on -lys, mates poorly" 11 F 8 YDR338C - - Putative protein of unknown function; member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily response to chemical transmembrane transporter activity membrane 4177 BY4741 04_7 11 F 9 YDR340W - - Putative protein of unknown function biological_process molecular_function cellular_component 4179 BY4741 04_7 11 F 10 YDR344C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4181 BY4741 04_7 11 F 11 YDR345C HXT3 hexose transporter HXT3 Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication transmembrane transport, carbohydrate transport transmembrane transporter activity membrane, plasma membrane 4182 BY4741 04_7 11 F 12 YDR346C SVF1 SGI1 Protein with a potential role in cell survival pathways; required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance response to chemical, response to oxidative stress molecular_function nucleus, cytoplasm 4183 BY4741 04_7 11 G 1 YDR347W MRP1 mitochondrial 37S ribosomal protein MRP1 Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4184 BY4741 04_7 11 G 2 YDR348C PAL1 - Protein of unknown function thought to be involved in endocytosis; physically interacts with Ede1p and is found at endocytic sites at cell periphery during early stages of endocytosis; green fluorescent protein (GFP)-fusion protein localizes to bud neck; potential Cdc28p substrate; similar to S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress; PAL1 has a paralog, YHR097C, that arose from the whole genome duplication biological_process molecular_function cellular bud, cell cortex, site of polarized growth, cytoplasm 4185 BY4741 04_7 11 G 3 YDR349C YPS7 - Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum cell wall organization or biogenesis peptidase activity, hydrolase activity endomembrane system, endoplasmic reticulum, cytoplasm 4186 BY4741 04_7 11 G 4 YDR350C ATP22 TCM10 Specific translational activator for the mitochondrial ATP6 mRNA; Atp6p encodes a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other mitochondrion, membrane, mitochondrial envelope, cytoplasm 4187 BY4741 04_7 11 G 5 YDR351W SBE2 - Protein required for bud growth; involved in transport of cell wall components from the Golgi to the cell surface; SBE2 has a paralog, SBE22, that arose from the whole genome duplication cell wall organization or biogenesis molecular_function endomembrane system, cytoplasm, Golgi apparatus 4188 BY4741 04_7 11 G 6 YDR352W YPQ2 - Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter transmembrane transport, ion transport, amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm 4189 BY4741 04_7 11 G 7 YDR354W TRP4 anthranilate phosphoribosyltransferase Anthranilate phosphoribosyl transferase; transferase of the tryptophan biosynthetic pathway; catalyzes the phosphoribosylation of anthranilate; subject to the general control system of amino acid biosynthesis cellular amino acid metabolic process transferase activity, transferring glycosyl groups, transferase activity nucleus, cytoplasm 4191 BY4741 04_7 11 G 8 YDR357C CNL1 BLC1 Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm other molecular_function cytoplasm 4194 BY4741 04_7 11 G 9 YDR358W GGA1 - Golgi-localized protein with homology to gamma-adaptin; interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi; GGA1 has a paralog, GGA2, that arose from the whole genome duplication Golgi vesicle transport, protein targeting, endosomal transport, proteolysis involved in cellular protein catabolic process ubiquitin-like protein binding endomembrane system, cytoplasm, Golgi apparatus 4195 BY4741 04_7 11 G 10 YDR359C EAF1 VID21 Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 DNA repair, chromatin organization, cellular response to DNA damage stimulus, protein complex biogenesis molecular_function nucleus 4196 BY4741 04_7 11 G 11 YDR360W OPI7 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C biological_process molecular_function cellular_component 4197 BY4741 04_7 11 G 12 YDR363W ESC2 - Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member peptidyl-amino acid modification, mitotic cell cycle, DNA repair, organelle fission, chromatin organization, cellular response to DNA damage stimulus, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, DNA recombination, regulation of cell cycle molecular_function nucleus 4200 BY4741 04_7 11 H 1 YDR364C CDC40 SLU4|SLT15|PRP17 Pre-mRNA splicing factor; important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression; required for DNA synthesis during mitosis and meiosis; has WD repeats; thermosensitivity of the cdc40 null mutant is functionally complemented by a chimeric construct containing the N-terminal 156 amino acids of yeast Cdc40p fused to the C-terminal two thirds (297 amino acids) of human CDC40 mitotic cell cycle, RNA splicing, mRNA processing other nucleus 4201 BY4741 04_7 11 H 3 YDR368W YPR1 trifunctional aldehyde reductase/carbonyl reductase (NADPH)/glucose 1-dehydrogenase (NADP(+)) YPR1 NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication; human homolog AKR1B1 can complement yeast null mutant carbohydrate metabolic process, response to chemical, response to oxidative stress oxidoreductase activity nucleus, cytoplasm 4204 BY4741 04_7 11 H 4 YDR369C XRS2 - Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling meiotic cell cycle, DNA repair, telomere organization, organelle fission, sporulation, cellular response to DNA damage stimulus, DNA recombination protein binding, bridging, DNA binding nucleus 4205 BY4741 04_7 11 H 5 YDR370C DXO1 - mRNA 5'-end-capping quality-control protein; has distributive, 5'-3' exoRNase activity; similar to Rai1p; RNA catabolic process nuclease activity, hydrolase activity cytoplasm 4206 BY4741 04_7 11 H 6 YDR371W CTS2 - Putative chitinase; functionally complements A. gossypii cts2 mutant sporulation defect sporulation molecular_function cytoplasm 4207 BY4741 04_7 11 H 7 YDR372C VPS74 MNN3|API1 Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 response to chemical, Golgi vesicle transport, lipid metabolic process, signaling lipid binding, enzyme binding, ion binding nucleus, endomembrane system, membrane, Golgi apparatus, cytoplasm 4208 BY4741 04_7 11 H 8 YDR374C PHO92 mRNA-binding phosphate metabolism regulator Posttranscriptional regulator of phosphate metabolism; facilitates PHO4 mRNA degradation by interacting with Pop2p; regulates PHO4 mRNA stability by binding to PHO4's 3'UTR in a phosphate-dependent manner; contains highly conserved YTH (YT521-B Homology) domain that exhibits RNA-binding activity; human homolog YTHDF2 can complement yeast null mutant other RNA binding, mRNA binding cytoplasm 4210 BY4741 04_7 11 H 9 YDR375C BCS1 bifunctional AAA family ATPase chaperone/translocase BCS1 Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane and delivers it to an assembly intermediate of respiratory Complex III; also required for assembly of the Qcr10p subunit; mutation is functionally complemented by human homolog BCS1L, linked to neonatal diseases mitochondrion organization, protein complex biogenesis protein transporter activity, hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 4211 BY4741 04_7 11 H 10 YDR377W ATP17 F1F0 ATP synthase subunit f Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis transmembrane transport, ion transport, nucleobase-containing small molecule metabolic process hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 4213 BY4741 04_7 11 H 11 YDR378C LSM6 U4/U6-U5 snRNP complex subunit LSM6 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA RNA splicing, rRNA processing, ribosomal small subunit biogenesis, mRNA processing RNA binding nucleus, nucleolus, cytoplasm 4214 BY4741 04_7 11 H 12 YDR379W RGA2 - GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p; RGA2 has a paralog, RGA1, that arose from the whole genome duplication cytoskeleton organization, signaling enzyme regulator activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 4215 BY4741 04_7 12 A 1 YDR380W ARO10 phenylpyruvate decarboxylase ARO10 Phenylpyruvate decarboxylase; catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway; involved in protein N-terminal Met and Ala catabolism cellular amino acid metabolic process lyase activity cytoplasm 4216 BY4741 04_7 12 A 2 YDR382W RPP2B ribosomal protein P2B|YP2beta|P2B|L45|YPA1|RPL45 Ribosomal protein P2 beta; a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P2 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm cytoplasmic translation, regulation of protein modification process, protein phosphorylation structural molecule activity, structural constituent of ribosome, enzyme regulator activity ribosome, cytoplasm 4218 BY4741 04_7 12 A 3 YDR383C NKP1 - Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 biological_process molecular_function chromosome 4219 BY4741 04_7 12 A 4 YDR384C ATO3 putative ammonium permease ATO3 Plasma membrane protein, putative ammonium transporter; regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm 4220 BY4741 04_7 12 A 5 YDR385W EFT2 elongation factor 2 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication regulation of translation, translational elongation RNA binding, translation factor activity, RNA binding ribosome, cytoplasm 4221 BY4741 04_7 12 A 6 YDR386W MUS81 SLX3 Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); helix-hairpin-helix protein; phosphorylation of non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates function of Mms4p-Mus81p meiotic cell cycle, DNA repair, organelle fission, cellular response to DNA damage stimulus, chromosome segregation, DNA recombination hydrolase activity, enzyme regulator activity, nuclease activity nucleus 4222 BY4741 04_7 12 A 7 YDR387C - - Putative transporter; member of the sugar porter family; YDR387C is not an essential gene transmembrane transport transmembrane transporter activity membrane 4223 BY4741 04_7 12 A 8 YDR388W RVS167 amphiphysin Actin-associated protein with roles in endocytosis and exocytosis; N-BAR domain protein that interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin endocytosis cytoskeletal protein binding, lipid binding cell cortex, site of polarized growth, cytoskeleton, cytoplasm 4224 BY4741 04_7 12 A 9 YDR389W SAC7 - GTPase activating protein (GAP) for Rho1p; regulator of a Tor2p-mediated, Rho1p GTPase switch that controls organization of the actin cytoskeleton; negative regulator of the RHO1-PKC1-MAPK cell integrity (CWI) and membrane fluidity homeostasis signaling pathways; potential Cdc28p substrate; SAC7 has a paralog, BAG7, that arose from the whole genome duplication cytoskeleton organization, regulation of organelle organization, cell wall organization or biogenesis, signaling enzyme regulator activity cell cortex, cytoplasm 4225 BY4741 04_7 12 A 10 YDR391C - - Putative protein of unknown function; possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 4227 BY4741 04_7 12 A 11 YDR392W SPT3 transcriptional regulator SPT3 Subunit of the SAGA and SAGA-like transcriptional regulatory complexes; interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters; relocalizes to the cytosol in response to hypoxia peptidyl-amino acid modification, protein acylation, invasive growth in response to glucose limitation, chromatin organization, histone modification, pseudohyphal growth, transcription from RNA polymerase II promoter transcription factor activity, protein binding nucleus, cytoplasm 4228 BY4741 04_7 12 B 1 YDR393W SHE9 MDM33 Protein required for normal mitochondrial morphology; mitochondrial inner membrane protein; may be involved in fission of the inner membrane; forms a homo-oligomeric complex mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4229 BY4741 04_7 12 B 2 YDR395W SXM1 KAP108 Nuclear transport factor (karyopherin); involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 nucleobase-containing compound transport, nuclear transport protein transporter activity nucleus, membrane, cytoplasm 4231 BY4741 04_7 12 B 3 YDR399W HPT1 hypoxanthine phosphoribosyltransferase|HGPRTase|HPRT|BRA6 Dimeric hypoxanthine-guanine phosphoribosyltransferase; catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups, transferase activity nucleus, cytoplasm 4235 BY4741 04_7 12 B 4 YDR400W URH1 trifunctional uridine nucleosidase/nicotinamide riboside hydrolase/nicotinic acid riboside hydrolase Uridine nucleosidase (uridine-cytidine N-ribohydrolase); cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways cofactor metabolic process, nucleobase-containing small molecule metabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds nucleus, cytoplasm 4236 BY4741 04_7 12 B 5 YDR401W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4237 BY4741 04_7 12 B 6 YDR402C DIT2 putative cytochrome P450|CYP56 N-formyltyrosine oxidase; sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s meiotic cell cycle, sporulation, cell wall organization or biogenesis oxidoreductase activity cellular_component 4238 BY4741 04_7 12 B 7 YDR403W DIT1 - Sporulation-specific enzyme required for spore wall maturation; involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure meiotic cell cycle, sporulation, cell wall organization or biogenesis other cellular_component 4239 BY4741 04_7 12 B 8 YDR405W MRP20 mitochondrial 54S ribosomal protein YmL41|YmL41|MRPL41 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4241 BY4741 04_7 12 B 9 YDR406W PDR15 ATP-binding cassette multidrug transporter PDR15 Plasma membrane ATP binding cassette (ABC) transporter; multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element; PDR15 has a paralog, PDR5, that arose from the whole genome duplication response to chemical ATPase activity, hydrolase activity, transmembrane transporter activity membrane 4242 BY4741 04_7 12 B 10 YDR408C ADE8 phosphoribosylglycinamide formyltransferase Phosphoribosyl-glycinamide transformylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway nucleobase-containing small molecule metabolic process transferase activity nucleus, cytoplasm 4244 BY4741 04_7 12 B 11 YDR409W SIZ1 SUMO ligase SIZ1|ULL1 SUMO/Smt3 ligase; promotes attachment of small ubiquitin-related modifier sumo (Smt3p) to proteins; regulates Rsp5p ubiquitin ligase activity and is in turn itself regulated by Rsp5p; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring; SIZ1 has a paralog, NFI1, that arose from the whole genome duplication peptidyl-amino acid modification, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal transferase activity nucleus, cell cortex, cytoskeleton, chromosome, cytoplasm 4245 BY4741 04_7 12 B 12 YDR410C STE14 protein-S-isoprenylcysteine carboxyl O-methyltransferase Farnesyl cysteine-carboxyl methyltransferase; mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane protein alkylation, protein maturation transferase activity, methyltransferase activity nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 4246 BY4741 04_7 Sterile [expected phenotype] 12 C 1 YDR411C DFM1 - Endoplasmic reticulum (ER) localized protein; involved in ER-associated protein degradation (ERAD), ER stress, and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p response to chemical, proteolysis involved in cellular protein catabolic process, signaling molecular_function endomembrane system, membrane, endoplasmic reticulum, cytoplasm 4247 BY4741 04_7 12 C 2 YDR414C ERD1 LDB2 Predicted membrane protein required for lumenal ER protein retention; mutants secrete the endogenous ER protein, BiP (Kar2p) protein glycosylation, carbohydrate metabolic process molecular_function membrane 4250 BY4741 04_7 12 C 3 YDR415C - putative aminopeptidase Putative aminopeptidase biological_process molecular_function cellular_component 4251 BY4741 04_7 12 C 4 YDR418W RPL12B ribosomal 60S subunit protein L12B|L11|YL23|L15B|L12B Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 4254 BY4741 04_7 12 C 5 YDR419W RAD30 DNA-directed DNA polymerase eta|DBH1 DNA polymerase eta; involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; involved in formation of post-replicative damage-induced genome-wide cohesion; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV mitotic cell cycle, DNA repair, organelle fission, cellular response to DNA damage stimulus, chromosome segregation transferase activity, nucleotidyltransferase activity nucleus, chromosome, mitochondrion, cytoplasm 4255 BY4741 04_7 12 C 6 YDR420W HKR1 - Mucin family member that functions as an osmosensor in the HOG pathway; functions in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection mitotic cell cycle, carbohydrate metabolic process, cytokinesis, cell wall organization or biogenesis, signaling, response to osmotic stress signal transducer activity membrane, site of polarized growth, plasma membrane 4256 BY4741 04_7 12 C 7 YDR421W ARO80 - Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus 4257 BY4741 04_7 12 C 8 YDR422C SIP1 - Alternate beta-subunit of the Snf1p kinase complex; may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions protein phosphorylation, signaling, protein complex biogenesis kinase activity, transferase activity vacuole, cytoplasm 4258 BY4741 04_7 12 C 9 YDR423C CAD1 YAP2 AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 4259 BY4741 04_7 12 C 10 YDR425W SNX41 - Sorting nexin; involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p endosomal transport ion binding, lipid binding endomembrane system, cytoplasm 4261 BY4741 04_7 12 C 11 YDR426C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 biological_process molecular_function cellular_component 4262 BY4741 04_7 12 C 12 YDR428C BNA7 arylformamidase Formylkynurenine formamidase; involved in the de novo biosynthesis of NAD from tryptophan via kynurenine cofactor metabolic process, nucleobase-containing small molecule metabolic process hydrolase activity cellular_component 4264 BY4741 04_7 12 D 1 YDR430C CYM1 MOP112 Lysine-specific metalloprotease of the pitrilysin family; metalloprotease of the intermembrane space; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology proteolysis involved in cellular protein catabolic process peptidase activity, hydrolase activity mitochondrial envelope, mitochondrion, cytoplasm 4266 BY4741 04_7 12 D 2 YDR431W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4267 BY4741 04_7 12 D 3 YDR432W NPL3 mRNA-binding protein NPL3|NAB1|NOP3|MTS1|MTR13 RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ubiquitin ligase Bre1p, linking histone ubiquitination to mRNA processing; may have role in telomere maintenance; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in cytoplasm; protein abundance increases in response to DNA replication stress RNA splicing, regulation of translation, nucleobase-containing compound transport, DNA-templated transcription, termination, mRNA processing, transcription from RNA polymerase II promoter, nuclear transport, DNA-templated transcription, elongation RNA binding, enzyme binding, mRNA binding nucleus, cytoplasm 4268 BY4741 04_7 12 D 4 YDR433W - KRE22 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data cellular response to DNA damage stimulus molecular_function cellular_component 4269 BY4741 04_7 12 D 5 YDR435C PPM1 leucine carboxy methyltransferase Carboxyl methyltransferase; methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits; required for methionine to inhibit autophagy and promote growth protein alkylation, protein complex biogenesis transferase activity, methyltransferase activity cellular_component 4271 BY4741 04_7 12 D 6 YDR436W PPZ2 - Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance cellular ion homeostasis phosphatase activity, hydrolase activity cellular_component 4272 BY4741 04_7 12 D 7 YEL001C IRC22 - Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm 241 BY4741 05_2 12 D 8 YEL003W GIM4 tubulin-binding prefolding complex subunit GIM4|PFD2 Subunit of the heterohexameric cochaperone prefoldin complex; complex binds specifically to cytosolic chaperonin and transfers target proteins to it protein complex biogenesis cytoskeletal protein binding cytoplasm 243 BY4741 05_2 12 D 9 YEL004W YEA4 - Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter; required for cell wall chitin synthesis; localized to the ER carbohydrate metabolic process, transmembrane transport, ion transport, nucleobase-containing compound transport, cell wall organization or biogenesis transmembrane transporter activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 244 BY4741 05_2 "Similar to K. lactis golgi uridine diphosphate-N-acetylglucosamine transporter -- growth on -met, no growth on -lys, no growth on drop-in media, confirmed alpha -- CORRECT STRAIN CAN BE FOUND IN PLATE 121 D6" 12 D 10 YEL005C VAB2 VAB31 Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Vps21p-GFP; has potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of; Vab2p-GFP-fusion localizes to cytoplasm in punctate pattern other molecular_function cytoplasm 245 BY4741 05_2 12 D 11 YEL006W YEA6 NDT2 Putative mitochondrial NAD+ transporter; member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog; YEA6 has a paralog, YIA6, that arose from the whole genome duplication transmembrane transport, nucleobase-containing compound transport other membrane, mitochondrial envelope, mitochondrion, cytoplasm 246 BY4741 05_2 12 D 12 YEL007W MIT1 TOS9 Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 biological_process molecular_function nucleus, cytoplasm 247 BY4741 05_2 12 E 1 YEL008W - - Hypothetical protein predicted to be involved in metabolism biological_process molecular_function cellular_component 248 BY4741 05_2 12 E 2 YEL009C GCN4 amino acid starvation-responsive transcription factor GCN4|AAS101|ARG9|AAS3 bZIP transcriptional activator of amino acid biosynthetic genes; activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels response to chemical, protein complex biogenesis, DNA-templated transcription, initiation, response to starvation, transcription from RNA polymerase II promoter chromatin binding, nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding nucleus 249 BY4741 05_2 12 E 3 YEL010W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 250 BY4741 05_2 12 E 4 YEL013W VAC8 protein anchor VAC8|YEB3 Phosphorylated and palmitoylated vacuolar membrane protein; interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions vacuole organization, protein targeting, organelle assembly, organelle fusion, vesicle organization, membrane fusion, membrane invagination, organelle inheritance other vacuole, membrane, cytoplasm 253 BY4741 05_2 12 E 5 YEL014C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 254 BY4741 05_2 12 E 6 YEL015W EDC3 LSM16|DCP3 Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; mediates decay of the RPS28B mRNA via binding to both Rps28Bp (or Rps28Ap) and the RPS28B mRNA; mediates decay of the YRA1 mRNA by a different, translation-independent mechanism; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress RNA catabolic process, organelle assembly RNA binding, mRNA binding nucleus, cytoplasm 255 BY4741 05_2 12 E 7 YEL016C NPP2 nucleotide diphosphatase/phosphodiesterase NPP2 Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; involved in spore wall assembly; NPP2 has a paralog, NPP1, that arose from the whole genome duplication, and an npp1 npp2 double mutant exhibits reduced dityrosine fluorescence relative to the single mutants nucleobase-containing small molecule metabolic process nuclease activity, hydrolase activity cellular_component 256 BY4741 05_2 12 E 8 YEL017C-A PMP2 - Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication ion transport molecular_function membrane 257 BY4741 05_2 12 E 9 YEL017W GTT3 - Protein of unknown function may be involved in glutathione metabolism; function suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery biological_process molecular_function cellular_component 258 BY4741 05_2 12 E 10 YPR083W MDM36 - Mitochondrial protein; required for normal mitochondrial morphology and inheritance; component of the mitochondria-ER-cortex-ancor (MECA); interacts with Num1p to link the ER and mitochondria at the cell cortex; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission organelle fission, organelle inheritance, mitochondrion organization molecular_function mitochondrion, cytoplasm 7401 BY4741 00_18 12 E 11 YEL020C - putative indolepyruvate decarboxylase family protein Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data biological_process molecular_function cytoplasm 261 BY4741 05_2 12 E 12 YEL023C - - Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene biological_process molecular_function cellular_component 264 BY4741 05_2 12 F 1 YEL024W RIP1 ubiquinol--cytochrome-c reductase catalytic subunit RIP1 Ubiquinol-cytochrome-c reductase; a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm 265 BY4741 05_2 12 F 2 YEL025C - SRI1 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 266 BY4741 05_2 12 F 3 YEL027W VMA3 H(+)-transporting V0 sector ATPase subunit c|CUP5|GEF2|CLS7 Proteolipid subunit c of the V0 domain of vacuolar H(+)-ATPase; dicyclohexylcarbodiimide binding subunit; required for vacuolar acidification and important for copper and iron metal ion homeostasis vacuole organization, protein targeting, ion transport, cellular ion homeostasis, endocytosis transmembrane transporter activity vacuole, membrane, cytoplasm 268 BY4741 05_2 12 F 4 YEL028W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 269 BY4741 05_2 12 F 5 YEL030W ECM10 Hsp70 family ATPase ECM10|SSC3 Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication protein targeting, protein folding, mitochondrion organization molecular_function mitochondrion, cytoplasm 271 BY4741 05_2 12 F 6 YEL031W SPF1 ion-transporting P-type ATPase SPF1|PER9|PIO1|COD1 P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 transmembrane transport, cellular ion homeostasis hydrolase activity, transmembrane transporter activity, ATPase activity endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm 272 BY4741 05_2 12 F 7 YEL033W MTC7 - Protein of unknown function; predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant biological_process molecular_function cellular_component 274 BY4741 05_2 12 F 8 YPR091C NVJ2 - Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial-lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments biological_process lipid binding endomembrane system, ribosome, endoplasmic reticulum, cytoplasm 7403 BY4741 00_18 12 F 9 YEL037C RAD23 - Protein with ubiquitin-like N terminus; subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover; Rad4p-Rad23p heterodimer binds to promoters of DNA damage response genes to repress their transcription in the absence of DNA damage response to chemical, proteolysis involved in cellular protein catabolic process, transcription from RNA polymerase II promoter protein binding, bridging, hydrolase activity, DNA binding, ubiquitin-like protein binding nucleus, mitochondrion, cytoplasm 278 BY4741 05_2 12 F 10 YEL038W UTR4 putative acireductone synthase UTR4 Protein with sequence similarity to acireductone synthases; involved in methionine salvage; found in both the cytoplasm and nucleus cellular amino acid metabolic process phosphatase activity, hydrolase activity nucleus, cytoplasm 279 BY4741 05_2 12 F 11 YEL039C CYC7 cytochrome c isoform 2 Cytochrome c isoform 2, expressed under hypoxic conditions; also known as iso-2-cytochrome c; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, cellular respiration other mitochondrial envelope, mitochondrion, cytoplasm 280 BY4741 05_2 12 F 12 YEL040W UTR2 CRH2 Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck carbohydrate metabolic process, cell wall organization or biogenesis transferase activity, transferring glycosyl groups, transferase activity cell cortex, cytoskeleton, cell wall, cellular bud, site of polarized growth, cytoplasm 281 BY4741 05_2 12 G 1 YEL041W YEF1 - ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication cofactor metabolic process, nucleobase-containing small molecule metabolic process kinase activity, transferase activity cellular_component 282 BY4741 05_2 12 G 2 YEL042W GDA1 guanosine diphosphatase Guanosine diphosphatase located in the Golgi; involved in the transport of GDP-mannose into the Golgi lumen, converting GDP to GMP after mannose is transferred to substrates; null mutants are defective in sporulation and pre-meiotic S phase entry; orthologous to human ENTPD6, a meiosis-associated non-obstructive azoospermia (NOA) related gene identified in GWAS studies protein glycosylation, carbohydrate metabolic process hydrolase activity endomembrane system, cytoplasm, Golgi apparatus 283 BY4741 05_2 12 G 3 YEL043W - - Predicted cytoskeleton protein involved in intracellular signaling; based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold biological_process molecular_function endomembrane system, ribosome, endoplasmic reticulum, cytoplasm 284 BY4741 05_2 12 G 4 YEL045C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress biological_process molecular_function membrane 286 BY4741 05_2 "Similar to cytochrome c oxidase III of T. brucei kinetoplast -- no growth on -met, super slow growth on -lys, super slow growth on drop in media" 12 G 5 YEL046C GLY1 threonine aldolase GLY1 Threonine aldolase; catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis cellular amino acid metabolic process lyase activity cytoplasm 287 BY4741 05_2 "L-threonine aldolase -- no growth on -met, super slow growth on -lys, super slow growth on drop in media" 12 G 6 YEL047C FRD1 FRDS1 Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication response to chemical oxidoreductase activity membrane, ribosome, mitochondrion, plasma membrane, cytoplasm 288 BY4741 05_2 12 G 7 YEL048C TCA17 - Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; promotes association of TRAPPII-specific subunits with the TRAPP core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder protein complex biogenesis, Golgi vesicle transport, endosomal transport molecular_function endomembrane system, cytoplasmic membrane-bounded vesicle, Golgi apparatus, cytoplasm 289 BY4741 05_2 12 G 8 YEL049W PAU2 seripauperin PAU2 Member of the seripauperin multigene family; encoded mainly in subtelomeric region; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme biological_process molecular_function cellular_component 290 BY4741 05_2 12 G 9 YEL050C RML2 mitochondrial 54S ribosomal protein RML2 Mitochondrial ribosomal protein of the large subunit (L2); has similarity to E. coli L2 ribosomal protein; mutant allele (fat21) causes inability to utilize oleate, and induce oleic acid oxidation; may interfere with activity of the Adr1p transcription factor mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 291 BY4741 05_2 12 G 10 YEL051W VMA8 H(+)-transporting V1 sector ATPase subunit D Subunit D of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits cellular ion homeostasis ATPase activity, hydrolase activity, transmembrane transporter activity membrane, vacuole, cytoplasm 292 BY4741 05_2 12 G 11 YEL052W AFG1 - Protein that may act as a chaperone for cytochrome c oxidase subunits; conserved protein; may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain response to chemical, protein targeting, transmembrane transport, proteolysis involved in cellular protein catabolic process, peroxisome organization, response to oxidative stress molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm 293 BY4741 05_2 12 G 12 YEL053C MAK10 NAA35 Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; expression is glucose-repressible protein acylation transferase activity cytoplasm 294 BY4741 05_2 12 H 1 YEL054C RPL12A ribosomal 60S subunit protein L12A|L11|YL23|L15A|L12A Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 295 BY4741 05_2 12 H 3 YEL056W HAT2 - Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity, histone binding nucleus, cytoplasm 297 BY4741 05_2 12 H 4 YEL057C SDD1 - Protein of unknown function; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P; may have a role in telomere maintenance; target of UME6 regulation biological_process molecular_function cellular_component 298 BY4741 05_2 12 H 5 YEL059W HHY1 ENV6 Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking biological_process molecular_function membrane 301 BY4741 05_2 12 H 6 YEL060C PRB1 proteinase B|CVT1 Vacuolar proteinase B (yscB) with H3 N-terminal endopeptidase activity; serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress; PRB1 has a paralog, YSP3, that arose from the whole genome duplication sporulation, response to starvation peptidase activity, hydrolase activity vacuole, cytoplasm 302 BY4741 05_2 12 H 7 YEL061C CIN8 kinesin motor protein CIN8|SDS15|KSL2 Kinesin motor protein; involved in mitotic spindle assembly and chromosome segregation mitotic cell cycle, organelle fission, cytoskeleton organization, organelle assembly, protein complex biogenesis, chromosome segregation hydrolase activity, ATPase activity nucleus, cytoskeleton, chromosome, mitochondrion, cytoplasm 303 BY4741 05_2 12 H 8 YEL062W NPR2 nitrogen permease regulating protein NPR2 Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of the SEA complex, a coatomer-related complex that associates dynamically with the vacuole; Npr2p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and resulting in inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; homolog of human tumor suppressor NPRL2 vacuole organization, response to chemical, regulation of organelle organization, organelle assembly, ion transport, response to starvation, amino acid transport, signaling molecular_function vacuole, membrane, cytoplasm 304 BY4741 05_2 12 H 9 YEL063C CAN1 arginine permease CAN1 Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication transmembrane transport, ion transport, amino acid transport transmembrane transporter activity mitochondrion, membrane, cytoplasm, plasma membrane 305 BY4741 05_2 12 H 10 YEL064C AVT2 - Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 306 BY4741 05_2 12 H 11 YEL065W SIT1 ARN3 Ferrioxamine B transporter; member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p cellular ion homeostasis, transmembrane transport transmembrane transporter activity membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm 307 BY4741 05_2 12 H 12 YEL066W HPA3 - D-Amino acid N-acetyltransferase that detoxifies D-amino acids; catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; acetylates histones and polyamines, also autoacetylates response to chemical, cellular amino acid metabolic process transferase activity nucleus, cytoplasm 308 BY4741 05_2 13 A 1 YEL067C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 309 BY4741 05_2 13 A 2 YEL068C - - Protein of unknown function; expressed at both mRNA and protein levels biological_process molecular_function cellular_component 310 BY4741 05_2 13 A 3 YEL071W DLD3 - D-lactate dehydrogenase; part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm monocarboxylic acid metabolic process oxidoreductase activity cytoplasm 313 BY4741 05_2 13 A 4 YEL072W RMD6 - Protein required for sporulation biological_process molecular_function cellular_component 314 BY4741 05_2 Hypothetical protein -- mates like alpha PCR mating type alpha 13 A 5 YER001W MNN1 alpha-1,3-mannosyltransferase MNN1 Alpha-1,3-mannosyltransferase; integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family carbohydrate metabolic process, protein glycosylation, oligosaccharide metabolic process transferase activity, transferring glycosyl groups, transferase activity endomembrane system, Golgi apparatus, cytoplasm 322 BY4741 05_2 13 A 6 YER002W NOP16 - Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleus, nucleolus 323 BY4741 05_2 13 A 7 YER004W FMP52 - Protein of unknown function; localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation biological_process molecular_function membrane, endomembrane system, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm 325 BY4741 05_2 13 A 8 YER005W YND1 apyrase|YEJ5|APY1 Apyrase with wide substrate specificity; helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity carbohydrate metabolic process, protein glycosylation hydrolase activity endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, Golgi apparatus, cytoplasm 326 BY4741 05_2 13 A 9 YER007C-A TMA20 RBF20 Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress other RNA binding ribosome, cytoplasm 328 BY4741 05_2 13 A 10 YER007W PAC2 - Microtubule effector required for tubulin heterodimer formation; binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl protein folding, protein complex biogenesis cytoskeletal protein binding cellular_component 329 BY4741 05_2 13 A 11 YER010C - - Bifunctional HMG aldolase/oxaloacetate decarboxylase; requires divalent metal ions for activity; competitively inhibited by oxalate; forms a ring-shaped homotrimer; similar to members of the prokaryotic RraA family of class II (divalent metal ion dependent) pyruvate aldolases from the meta cleavage pathways of protocatechuate and gallate monocarboxylic acid metabolic process lyase activity cellular_component 332 BY4741 05_2 13 A 12 YER011W TIR1 SRP1 Cell wall mannoprotein; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking; member of the Srp1p/Tip1p family of serine-alanine-rich proteins biological_process structural molecule activity cell wall 333 BY4741 05_2 13 B 2 YPR011C - - Mitochondrial transporter; major substrates are adenosine 5'-phosphosulfate (APS) and 3'-phospho-adenosine 5'-phosphosulfate (PAPS); member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies transmembrane transport, ion transport, nucleobase-containing compound transport transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 7399 BY4741 00_18 13 B 3 YER017C AFG3 AAA family ATPase AFG3|YTA10 Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging; expression of human homolog AFG3L2 can complement yeast yta12 afg3 double mutant cytoplasmic translation, protein maturation, protein complex biogenesis peptidase activity, hydrolase activity, ATPase activity vacuole, mitochondrion, membrane, mitochondrial envelope, cytoplasm 148 BY4741 05_3 13 B 4 YER019W ISC1 inositol phosphosphingolipid phospholipase Inositol phosphosphingolipid phospholipase C; mitochondrial membrane localized; hydrolyzes complex sphingolipids to produce ceramide; activates genes required for non-fermentable carbon source metabolism during diauxic shift; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance; ortholog of mammalian neutral sphingomyelinase type 2 lipid metabolic process, response to osmotic stress hydrolase activity endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm 150 BY4741 05_3 13 B 5 YER019C-A SBH2 Arf family guanine nucleotide exchange factor SBH2|SEB2 Ssh1p-Sss1p-Sbh2p complex component; involved in protein translocation into the endoplasmic reticulum; SBH2 has a paralog, SBH1, that arose from the whole genome duplication protein targeting guanyl-nucleotide exchange factor activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 151 BY4741 05_3 13 B 6 YER020W GPA2 guanine nucleotide-binding protein subunit alpha|SSP101 Nucleotide binding alpha subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p, has signaling role in response to nutrients; required for the recruitment of Ras-GTP at the plasma membrane and in the nucleus meiotic cell cycle, response to chemical, sporulation, pseudohyphal growth, signaling GTPase activity, hydrolase activity mitochondrion, membrane, cytoplasm, plasma membrane 152 BY4741 05_3 13 B 7 YER024W YAT2 carnitine O-acetyltransferase YAT2 Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane monocarboxylic acid metabolic process transferase activity cytoplasm 156 BY4741 05_3 13 B 8 YER030W CHZ1 - Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress chromatin organization histone binding nucleus 162 BY4741 05_3 13 B 9 YER032W FIR1 PIP1 Protein involved in 3' mRNA processing; interacts with Ref2p; APCC(Cdh1) substrate; potential Cdc28p substrate mRNA processing molecular_function cellular bud, site of polarized growth 164 BY4741 05_3 13 B 10 YER033C ZRG8 - Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency cell wall organization or biogenesis molecular_function cellular bud, site of polarized growth, mitochondrion, cytoplasm 165 BY4741 05_3 13 B 11 YER034W - - Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus, cytoplasm 166 BY4741 05_3 13 B 12 YER035W EDC2 - RNA-binding protein that directly activates mRNA decapping; binds mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein increases in abundance and relocalizes to nucleolus and to nuclear foci upon DNA replication stress; EDC2 has a paralog, EDC1, that arose from the whole genome duplication RNA catabolic process, regulation of translation RNA binding, mRNA binding nucleus, nucleolus, cytoplasm 167 BY4741 05_3 13 C 1 YER038W-A - - Mitochondrial protein of unknown function; almost completely overlaps ORF HVG1/YER039C biological_process molecular_function mitochondrion, cytoplasm 171 BY4741 05_3 13 C 2 YER039C HVG1 YEM9 Protein of unknown function; HVG1 has a paralog, VRG4, that arose from the whole genome duplication biological_process molecular_function membrane 172 BY4741 05_3 13 C 3 YER040W GLN3 nitrogen-responsive transcriptional regulator GLN3 Transcriptional activator of genes regulated by nitrogen catabolite repression; localization and activity regulated by quality of nitrogen source and Ure2p response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 173 BY4741 05_3 13 C 4 YER041W YEN1 - Holliday junction resolvase; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1; similar to S. cerevisiae endonuclease Rth1p DNA repair, cellular response to DNA damage stimulus nuclease activity, hydrolase activity nucleus, cytoplasm 174 BY4741 05_3 13 C 5 YER042W MXR1 peptide-methionine-S-sulfoxide reductase|msrA Methionine-S-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan; reduced activity of human homolog implicated in Alzheimer disease response to chemical, response to oxidative stress oxidoreductase activity nucleus, cytoplasm 175 BY4741 05_3 13 C 6 YER044C-A MEI4 - Meiosis-specific protein involved in forming DSBs; involved in double-strand break (DSBs) formation during meiotic recombination; required for chromosome synapsis and production of viable spores meiotic cell cycle, organelle fission molecular_function nucleus, chromosome 178 BY4741 05_3 13 C 7 YER045C ACA1 - ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 179 BY4741 05_3 13 C 8 YER046W-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SAP1/YER047C biological_process molecular_function cellular_component 181 BY4741 05_3 13 C 9 YER047C SAP1 putative AAA family ATPase SAP1 Putative ATPase of the AAA family; interacts with the Sin1p transcriptional repressor in the two-hybrid system biological_process ATPase activity, hydrolase activity cytoplasm 182 BY4741 05_3 13 C 10 YER048C CAJ1 - Nuclear type II J heat shock protein of the E. coli dnaJ family; contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly protein folding molecular_function nucleus 183 BY4741 05_3 13 C 11 YER049W TPA1 - Fe(II)/2-oxoglutarate-dependent dioxygenase family member; catalyzes the repair of methyl-base lesions in both ss and dsDNA by oxidative demethylation; Poly(rA)-binding protein involved in mRNA poly(A) tail length and mRNA stability; role in translation termination efficiency; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to human prolyl 4-hydroxylase OGFOD1; binds Fe(II) and 2-oxoglutarate RNA catabolic process, DNA repair, cellular response to DNA damage stimulus RNA binding, ion binding, oxidoreductase activity nucleus 184 BY4741 05_3 13 C 12 YER050C RSM18 mitochondrial 37S ribosomal protein RSM18 Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S18 ribosomal protein mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 185 BY4741 05_3 13 D 1 YER051W JHD1 [Histone H3]-lysine-36 demethylase|KDM2|JHDM1 JmjC domain family histone demethylase specific for H3-K36; similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe chromatin organization, transcription from RNA polymerase II promoter, histone modification, DNA-templated transcription, elongation histone binding, oxidoreductase activity cellular_component 186 BY4741 05_3 13 D 2 YER052C HOM3 aspartate kinase|THR3|SIL4|BOR1 Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis cellular amino acid metabolic process kinase activity, transferase activity cytoplasm 187 BY4741 05_3 13 D 3 YER053C PIC2 Cu/Pi carrier Mitochondrial copper and phosphate carrier; imports copper and inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature cellular ion homeostasis, transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrion, cytoplasm 188 BY4741 05_3 13 D 4 YER054C GIP2 - Putative regulatory subunit of protein phosphatase Glc7p; involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p; GIP2 has a paralog, PIG2, that arose from the whole genome duplication carbohydrate metabolic process, protein dephosphorylation, generation of precursor metabolites and energy enzyme regulator activity cytoplasm 189 BY4741 05_3 13 D 5 YER056C FCY2 BRA7 Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm 191 BY4741 05_3 13 D 6 YER056C-A RPL34A ribosomal 60S subunit protein L34A|L34e|L34A Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome other 192 BY4741 05_3 13 D 7 YER057C HMF1 putative isoleucine biosynthesis protein HMF1|HIG1 Member of the p14.5 protein family; functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro; HMF1 has a paralog, MMF1, that arose from the whole genome duplication biological_process molecular_function nucleus, mitochondrial envelope, mitochondrion, cytoplasm 193 BY4741 05_3 13 D 8 YER058W PET117 - Protein required for assembly of cytochrome c oxidase mitochondrion organization, protein complex biogenesis molecular_function mitochondrion, cytoplasm 194 BY4741 05_3 13 D 9 YER059W PCL6 - Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding; PCL6 has a paralog, PCL7, that arose from the whole genome duplication carbohydrate metabolic process, generation of precursor metabolites and energy enzyme regulator activity other 195 BY4741 05_3 13 D 10 YER060W FCY21 - Putative purine-cytosine permease; very similar to Fcy2p but cannot substitute for its function transmembrane transport transmembrane transporter activity membrane, plasma membrane 196 BY4741 05_3 13 D 11 YER060W-A FCY22 - Putative purine-cytosine permease; very similar to Fcy2p but cannot substitute for its function transmembrane transport transmembrane transporter activity membrane, plasma membrane 197 BY4741 05_3 "similar to Fcy2p -- growth on -met, no growth on -lys, no growth on drop-in media" 13 D 12 YER061C CEM1 fatty acid synthase CEM1 Mitochondrial beta-keto-acyl synthase; possible role in fatty acid synthesis; required for mitochondrial respiration; human homolog OXSM can complement yeast cem1 null mutant lipid metabolic process, monocarboxylic acid metabolic process transferase activity mitochondrion, cytoplasm 198 BY4741 05_3 13 E 1 YER062C GPP2 HOR2|glycerol-1-phosphatase HOR2 DL-glycerol-3-phosphate phosphatase involved in glycerol biosynthesis; also known as glycerol-1-phosphatase; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; GPP2 has a paralog, GPP1, that arose from the whole genome duplication carbohydrate metabolic process, response to osmotic stress phosphatase activity, hydrolase activity nucleus, cytoplasm 199 BY4741 05_3 13 E 2 YER065C ICL1 isocitrate lyase 1 Isocitrate lyase; catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose carbohydrate metabolic process, monocarboxylic acid metabolic process lyase activity cytoplasm 202 BY4741 05_3 13 E 3 YER066C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YER067W biological_process molecular_function cellular_component 204 BY4741 05_3 13 E 4 YER067W RGI1 - Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion or in response to DNA replication stress; RGI1 has a paralog, RGI2, that arose from the whole genome duplication generation of precursor metabolites and energy molecular_function nucleus, cytoplasm 205 BY4741 05_3 13 E 5 YER067C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF RGI1/YER067W biological_process molecular_function cellular_component 206 BY4741 05_3 13 E 6 YER068W MOT2 CCR4-NOT core ubiquitin-protein ligase subunit MOT2|SIG1|NOT4 Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication proteolysis involved in cellular protein catabolic process, RNA catabolic process, protein modification by small protein conjugation or removal, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation transferase activity, DNA binding cytoplasm 207 BY4741 05_3 13 E 7 YER068C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF ARG5,6/YER069W biological_process molecular_function cellular_component 208 BY4741 05_3 13 E 8 YER069W ARG5,6 bifunctional acetylglutamate kinase/N-acetyl-gamma-glutamyl-phosphate reductase Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine cellular amino acid metabolic process kinase activity, transferase activity, oxidoreductase activity mitochondrion, cytoplasm 209 BY4741 05_3 13 E 9 YPR118W MRI1 S-methyl-5-thioribose-1-phosphate isomerase MRI1 5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway cellular amino acid metabolic process isomerase activity nucleus, cytoplasm 7405 BY4741 00_18 13 E 10 YER071C TDA2 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function site of polarized growth, cytoplasm 211 BY4741 05_3 13 E 11 YER072W VTC1 PHM4|NRF1 Subunit of the vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity; protein abundance increases in response to DNA replication stress vacuole organization, organelle fusion, membrane fusion, membrane invagination RNA binding, mRNA binding endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm 212 BY4741 05_3 13 E 12 YER073W ALD5 aldehyde dehydrogenase (NAD(P)(+)) ALD5 Mitochondrial aldehyde dehydrogenase; involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed monocarboxylic acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 213 BY4741 05_3 13 F 1 YER074W RPS24A ribosomal 40S subunit protein S24A|S24e|S24A|RPS24EA Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm 214 BY4741 05_3 13 F 2 YER075C PTP3 tyrosine protein phosphatase PTP3 Phosphotyrosine-specific protein phosphatase; involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm response to chemical, protein phosphorylation, sporulation, protein dephosphorylation, regulation of protein modification process, conjugation, signaling, response to osmotic stress phosphatase activity, hydrolase activity nucleus, cytoplasm 215 BY4741 05_3 13 F 3 YER079W - - Putative protein of unknown function biological_process molecular_function nucleus, cytoplasm 219 BY4741 05_3 13 F 4 YER080W AIM9 FMP29 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 220 BY4741 05_3 13 F 5 YER081W SER3 phosphoglycerate dehydrogenase SER3 3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication cellular amino acid metabolic process oxidoreductase activity cytoplasm 221 BY4741 05_3 13 F 6 YER083C GET2 GET complex subunit GET2|RMD7|HUR2 Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Golgi vesicle transport other membrane, endomembrane system, endoplasmic reticulum, cytoplasm 223 BY4741 05_3 13 F 7 YER084W - - Protein of unknown function; expressed at both mRNA and protein levels biological_process molecular_function cellular_component 224 BY4741 05_3 13 F 8 YER085C - - Putative protein of unknown function biological_process molecular_function cellular_component 225 BY4741 05_3 13 F 9 YER086W ILV1 threonine ammonia-lyase ILV1|ISO1 Threonine deaminase, catalyzes first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation cellular amino acid metabolic process lyase activity mitochondrion, cytoplasm 226 BY4741 05_3 13 F 10 YER087C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF AIM10/YER087W biological_process molecular_function cellular_component 228 BY4741 05_3 13 F 11 YGR123C PPT1 - Protein serine/threonine phosphatase; regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth protein dephosphorylation phosphatase activity, hydrolase activity nucleus, cytoplasm 4753 BY4741 07_5 13 F 12 YGR124W ASN2 asparagine synthase (glutamine-hydrolyzing) 2 Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication cellular amino acid metabolic process ligase activity cytoplasm 4754 BY4741 07_5 13 G 1 YGR125W - - Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function membrane, vacuole, cytoplasm 4755 BY4741 07_5 13 G 2 YGR126W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus, cytoplasm 4756 BY4741 07_5 13 G 3 YGR127W - - Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response biological_process molecular_function cellular_component 4757 BY4741 07_5 13 G 4 YGR129W SYF2 NTC31 Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; relocalizes to the cytosol in response to hypoxia; isy1 syf2 cells have defective spindles activiating cell cycle arrest RNA splicing, mRNA processing other nucleus, cytoplasm 4759 BY4741 07_5 13 G 5 YGR130C - - Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) biological_process molecular_function membrane, cytoplasm 4760 BY4741 07_5 13 G 6 YGR131W FHN1 - Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain; FHN1 has a paralog, NCE102, that arose from the whole genome duplication other molecular_function membrane 4761 BY4741 07_5 13 G 7 YGR132C PHB1 prohibitin subunit PHB1 Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR) protein folding, organelle inheritance, mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4762 BY4741 07_5 13 G 8 YGR133W PEX4 E2 ubiquitin-protein ligase peroxin 4|UBC10|PAS2 Peroxisomal ubiquitin conjugating enzyme; required for peroxisomal matrix protein import and peroxisome biogenesis protein targeting, protein modification by small protein conjugation or removal, transmembrane transport, peroxisome organization, signaling transferase activity peroxisome, cytoplasm 4763 BY4741 07_5 13 G 9 YGR135W PRE9 proteasome core particle subunit alpha 3 Alpha 3 subunit of the 20S proteasome; the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform proteolysis involved in cellular protein catabolic process, protein complex biogenesis molecular_function nucleus, membrane, endomembrane system, cytoplasm 4765 BY4741 07_5 13 G 10 YGR136W LSB1 - Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with PIN3 cooperatively inhibits the nucleation of actin filaments; overexpression blocks receptor-mediated endocytosis; protein increases in abundance and forms nuclear foci in response to DNA replication stress; LSB1 has a paralog, PIN3, that arose from the whole genome duplication cytoskeleton organization, regulation of organelle organization, protein complex biogenesis molecular_function nucleus, cell cortex, cytoskeleton, cytoplasm 4766 BY4741 07_5 13 G 11 YGR137W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4767 BY4741 07_5 13 G 12 YGR138C TPO2 - Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication ion transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm 4768 BY4741 07_5 13 H 1 YGR139W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4769 BY4741 07_5 13 H 3 YGR141W VPS62 - Vacuolar protein sorting (VPS) protein; required for cytoplasm to vacuole targeting of proteins; VPS62 has a paralog, TDA6, that arose from the whole genome duplication protein targeting molecular_function membrane, vacuole, cytoplasm 4771 BY4741 07_5 13 H 4 YGR142W BTN2 - v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; contributes to prion curing; BTN2 has a paralog, CUR1, that arose from the whole genome duplication protein folding, endosomal transport, ion transport, amino acid transport other nucleus, endomembrane system, cytoplasm 4772 BY4741 07_5 13 H 5 YGR143W SKN1 beta-glucan synthesis-associated protein SKN1 Protein involved in sphingolipid biosynthesis; type II membrane protein; SKN1 has a paralog, KRE6, that arose from the whole genome duplication carbohydrate metabolic process, lipid metabolic process, cell wall organization or biogenesis hydrolase activity, hydrolase activity, acting on glycosyl bonds membrane 4773 BY4741 07_5 13 H 6 YGR144W THI4 thiamine thiazole synthase|MOL1|ESP35 Thiazole synthase; abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents mitochondrion organization, vitamin metabolic process ion binding cytoplasm 4774 BY4741 07_5 13 H 7 YGR146C ECL1 - Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation biological_process molecular_function cellular_component 4776 BY4741 07_5 13 H 8 YGR148C RPL24B ribosomal 60S subunit protein L24B|L24e|rp29|YL21|L30B|L24B|RPL30B Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 4778 BY4741 07_5 13 H 9 YGR149W - - Putative protein of unknown function; predicted to be an integal membrane protein biological_process molecular_function membrane 4779 BY4741 07_5 13 H 10 YGR150C CCM1 DMR1|RRG2 Mitochondrial 15S rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; has separable roles in stabilizing mitochondrial 15S rRNA and in maturation of the COB and COX1 mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport RNA splicing, mitochondrion organization RNA binding, rRNA binding mitochondrion, cytoplasm 4780 BY4741 07_5 13 H 11 YGR151C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus, cytoplasm 4781 BY4741 07_5 13 H 12 YGR152C RSR1 Ras family GTPase RSR1|BUD1 GTP-binding protein of the Ras superfamily; required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases mitotic cell cycle, cell budding, cytokinesis, conjugation, signaling GTPase activity, hydrolase activity, signal transducer activity cellular bud, membrane, site of polarized growth, plasma membrane 4782 BY4741 07_5 14 A 1 YGR153W - TOS10 Putative protein of unknown function biological_process molecular_function cellular_component 4783 BY4741 07_5 14 A 2 YGR154C GTO1 - Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization other transferase activity peroxisome, cytoplasm 4784 BY4741 07_5 14 A 3 YGR157W CHO2 phosphatidylethanolamine N-methyltransferase|PEM1 Phosphatidylethanolamine methyltransferase (PEMT); catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis lipid metabolic process transferase activity, methyltransferase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 4787 BY4741 07_5 14 A 4 YGR159C NSR1 SHE5 Nucleolar protein that binds nuclear localization sequences; required for pre-rRNA processing and ribosome biogenesis; binds to single stranded telomeric DNA and mRNA; methylated by Hmt1p; interaction with Top1p and nucleolar localization are negatively regulated by polyphosphorylation rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly RNA binding, DNA binding, mRNA binding nucleus, endomembrane system, mitochondrion, nucleolus, cytoplasm 4789 BY4741 07_5 14 A 5 YGR160W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4790 BY4741 07_5 14 A 6 YGR161C RTS3 - Putative component of the protein phosphatase type 2A complex biological_process molecular_function nucleus, cytoplasm 4791 BY4741 07_5 14 A 7 YGR163W GTR2 - Subunit of a TORC1-stimulating GTPase complex; subunit of the Gtr1-Gtr2 GTPase complex that stimulates TORC1 in response to amino acid stimulation; stimulates the GTPase activity of Gtr1p; negatively regulates the Ran/Tc4 GTPase cycle; activates transcription; tethered to the vacuolar membrane as part of the EGO complex (EGOC); required for sorting of Gap1p; activated by the the Lst4p-Lst7p GAP complex; localizes to cytoplasm and to chromatin; homolog of human RagC and membrane invagination, transcription from RNA polymerase II promoter, signaling GTPase activity, hydrolase activity, ion binding nucleus, endomembrane system, chromosome, vacuole, membrane, cytoplasm 4793 BY4741 07_5 14 A 8 YGR164W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4794 BY4741 07_5 14 A 9 YGR165W MRPS35 mitochondrial 37S ribosomal protein MRPS35 Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4795 BY4741 07_5 14 A 10 YGR166W TRS65 KRE11 Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; role in cell wall beta-glucan biosynthesis and the stress response Golgi vesicle transport, protein complex biogenesis guanyl-nucleotide exchange factor activity endomembrane system, cytoplasm, Golgi apparatus 4796 BY4741 07_5 14 A 11 YOL153C endomembrane system, cytoplasm, Golgi apparatus endomembrane system, cytoplasm, Golgi apparatus endomembrane system, cytoplasm, Golgi apparatus - - - 7133 BY4741 00_15 14 A 12 YGR168C - - Putative protein of unknown function; YGR168C is not an essential gene biological_process molecular_function membrane 4798 BY4741 07_5 "Hypothetical protein -- growth on -met, no growth on -lys, no growth on drop-in media" 14 B 1 YGR169C PUS6 pseudouridine synthase PUS6 tRNA:pseudouridine synthase; catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability tRNA processing, RNA modification isomerase activity mitochondrion, cytoplasm 4799 BY4741 07_5 14 B 3 YGR170W PSD2 phosphatidylserine decarboxylase 2 Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes; converts phosphatidylserine to phosphatidylethanolamine; controls vacuolar membrane phospholipid content by regulating phospholipids in compartments that will eventually give rise to the vacuole; loss of Psd2p causes a specific reduction in vacuolar membrane PE levels while total PE levels are not significantly affected lipid metabolic process lyase activity endomembrane system, cytoplasm 4800 BY4741 07_5 14 B 4 YGR171C MSM1 methionine--tRNA ligase MSM1 Mitochondrial methionyl-tRNA synthetase (MetRS); functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p tRNA aminoacylation for protein translation, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 4801 BY4741 07_5 14 B 5 YGR173W RBG2 GIR1 Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein cytoplasmic translation ion binding cytoplasm 4803 BY4741 07_5 14 B 6 YGR174C CBP4 - Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex mitochondrion organization, protein complex biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4804 BY4741 07_5 14 B 7 YGR176W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4806 BY4741 07_5 14 B 8 YGR177C ATF2 alcohol O-acetyltransferase Alcohol acetyltransferase; may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking response to chemical, generation of precursor metabolites and energy, lipid metabolic process transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 4807 BY4741 07_5 14 B 9 YGR178C PBP1 MRS16 Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress regulation of translation, organelle assembly, mRNA processing RNA binding, mRNA binding nucleus, mitochondrion, cytoplasm 4808 BY4741 07_5 14 B 10 YGR180C RNR4 ribonucleotide-diphosphate reductase subunit RNR4|PSO3|CRT3 Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress; RNR4 has a paralog, RNR2, that arose from the whole genome duplication cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity nucleus, cytoplasm 4810 BY4741 07_5 14 B 11 YGR181W TIM13 protein translocase subunit TIM13 Mitochondrial intermembrane space protein; forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane protein targeting, mitochondrion organization, transmembrane transport protein transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 4811 BY4741 07_5 14 B 12 YGR182C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W biological_process molecular_function cellular_component 4812 BY4741 07_5 14 C 1 YGR183C QCR9 ubiquinol--cytochrome-c reductase subunit 9|UCR9 Subunit 9 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex mitochondrion organization, generation of precursor metabolites and energy, protein complex biogenesis, cellular respiration, nucleobase-containing small molecule metabolic process transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 4813 BY4741 07_5 14 C 2 YGR184C UBR1 E3 ubiquitin-protein ligase UBR1|PTR1 E3 ubiquitin ligase (N-recognin); heterodimerizes with Rad6p to recognize and ubiquitinate substrates of the N-end rule pathway; role in endoplasmic reticulum-associated protein degradation (ERAD) in the absence of canonical ER membrane ligases or after stress; major role in targeting misfolded cytosolic proteins for degradation; regulates peptide transport via Cup9p ubiquitination; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) response to chemical, protein modification by small protein conjugation or removal, regulation of transport, proteolysis involved in cellular protein catabolic process transferase activity cytoplasm 4814 BY4741 07_5 14 C 3 YGR187C HGH1 - Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus, cytoplasm 4817 BY4741 07_5 14 C 4 YGR189C CRH1 - Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress carbohydrate metabolic process, cell wall organization or biogenesis transferase activity, transferring glycosyl groups, transferase activity cell wall 4819 BY4741 07_5 14 C 5 YGR192C TDH3 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) TDH3|GAPDH|GPD|SSS2|HSP36|HSP35|GLD1 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bacteria; binds AU-rich RNA carbohydrate metabolic process, cofactor metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, nucleobase-containing small molecule metabolic process RNA binding, oxidoreductase activity mitochondrion, cell wall, membrane, cytoplasm, plasma membrane 4822 BY4741 07_5 14 C 6 YGR193C PDX1 - E3-binding protein of the mitochondrial pyruvate dehydrogenase complex; plays a structural role in the complex by binding and positioning dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide acetyltransferase (E2) core cofactor metabolic process, monocarboxylic acid metabolic process structural molecule activity mitochondrion, cytoplasm 4823 BY4741 07_5 14 C 7 YGR194C XKS1 xylulokinase Xylulokinase; converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains carbohydrate metabolic process kinase activity, transferase activity cytoplasm 4824 BY4741 07_5 14 C 8 YGR196C FYV8 - Protein of unknown function; required for survival upon exposure to K1 killer toxin biological_process molecular_function cytoplasm 4826 BY4741 07_5 14 C 9 YGR197C SNG1 - Protein involved in resistance to nitrosoguanidine and 6-azauracil; expression is regulated by transcription factors involved in multidrug resistance; SNG1 has a paralog, YJR015W, that arose from the whole genome duplication nucleobase-containing compound transport molecular_function membrane, plasma membrane 4827 BY4741 07_5 14 C 10 YGR199W PMT6 dolichyl-phosphate-mannose-protein mannosyltransferase PMT6 Protein O-mannosyltransferase; transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups, transferase activity membrane 4829 BY4741 07_5 14 C 11 YGR200C ELP2 Elongator subunit ELP2|KTI3|TOT2 Subunit of Elongator complex; binds to microtubules via conserved alkaline residues; has two seven-bladed WD40 β propellers; Elongator complex is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin tRNA processing, protein modification by small protein conjugation or removal, transcription from RNA polymerase II promoter, RNA modification cytoskeletal protein binding nucleus, cytoplasm 4830 BY4741 07_5 14 C 12 YGR202C PCT1 choline-phosphate cytidylyltransferase|CCT1|BSR2 Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity lipid metabolic process transferase activity, nucleotidyltransferase activity nucleus, endomembrane system, cytoplasm, Golgi apparatus 4832 BY4741 07_5 14 D 1 YGR203W YCH1 phosphatase YCH1 Phosphatase with sequence similarity to Cdc25p; Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus other phosphatase activity, transferase activity, hydrolase activity nucleus, cytoplasm 4833 BY4741 07_5 14 D 2 YGR205W TDA10 putative ATP-dependent kinase ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele biological_process ion binding nucleus, cytoplasm 4835 BY4741 07_5 14 D 3 YGR206W MVB12 ubiquitin-binding ESCRT-I subunit protein MVB12 ESCRT-I subunit required to stabilize ESCRT-I core complex oligomers; the ESCRT-I core complex (Stp22p, Vps28p, Srn2p) is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin protein targeting, endosomal transport, protein complex biogenesis ubiquitin-like protein binding membrane, endomembrane system, cytoplasm 4836 BY4741 07_5 14 D 4 YGR207C CIR1 - Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response biological_process molecular_function mitochondrion, cytoplasm 4837 BY4741 07_5 14 D 5 YGR208W SER2 phosphoserine phosphatase Phosphoserine phosphatase of the phosphoglycerate pathway; involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source cellular amino acid metabolic process phosphatase activity, hydrolase activity nucleus, cytoplasm 4838 BY4741 07_5 14 D 6 YGR209C TRX2 thioredoxin TRX2|LMA1 Cytoplasmic thioredoxin isoenzyme; part of thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; with Trx1p, facilitates mitochondrial import of small Tims Tim9p, Tim10p, Tim13p by maintaining them in reduced form; abundance increases under DNA replication stress; TRX2 has a paralog, TRX1, that arose from the whole genome duplication vacuole organization, organelle fusion, Golgi vesicle transport, membrane fusion, organelle inheritance oxidoreductase activity vacuole, cytoplasm 4839 BY4741 07_5 14 D 7 YGR212W SLI1 - N-acetyltransferase; confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin response to chemical transferase activity nucleus, membrane, endomembrane system, plasma membrane 4842 BY4741 07_5 14 D 8 YGR213C RTA1 - Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein; RTA1 has a paralog, YLR046C, that arose from the whole genome duplication biological_process molecular_function membrane, plasma membrane 4843 BY4741 07_5 14 D 9 YGR214W RPS0A ribosomal 40S subunit protein S0A|S2|S0A|YST1|NAB1A|NAB1 Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; cytoplasmic translation, rRNA processing, organelle assembly, nucleobase-containing compound transport, ribosomal small subunit biogenesis, ribosome assembly, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 4844 BY4741 07_5 14 D 10 YGR215W RSM27 mitochondrial 37S ribosomal protein RSM27 Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4845 BY4741 07_5 14 D 11 YGR217W CCH1 - Voltage-gated high-affinity calcium channel; involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and partially co-localizes with Mid1p; however, evidence suggests CCH1 is not required for Mid1p function ion transport transmembrane transporter activity membrane, plasma membrane 4847 BY4741 07_5 14 D 12 YHL047C ARN2 TAF1 Transporter; member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C cofactor metabolic process, cellular ion homeostasis transmembrane transporter activity membrane, plasma membrane 916 BY4741 08_1 14 E 1 YHL046C PAU13 seripauperin PAU13 Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock biological_process molecular_function cellular_component 917 BY4741 08_1 14 E 2 YHL045W - - Putative protein of unknown function; not an essential gene biological_process molecular_function cellular_component 918 BY4741 08_1 14 E 3 YHL044W - DUP240 family protein Putative integral membrane protein; member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern biological_process molecular_function membrane, plasma membrane 919 BY4741 08_1 14 E 4 YHL043W ECM34 - Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins biological_process molecular_function cellular_component 920 BY4741 08_1 14 E 5 YHL042W - - Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins biological_process molecular_function cellular_component 921 BY4741 08_1 14 E 6 YHL041W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 922 BY4741 08_1 14 E 7 YHL040C ARN1 - ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress other transmembrane transporter activity nucleus, endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, cytoplasm, plasma membrane 923 BY4741 08_1 14 E 8 YHL038C CBP2 - Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene RNA splicing RNA binding mitochondrion, cytoplasm 925 BY4741 08_1 14 E 9 YHL037C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 926 BY4741 08_1 14 E 10 YHL036W MUP3 - Low affinity methionine permease; similar to Mup1p ion transport, amino acid transport transmembrane transporter activity membrane 927 BY4741 08_1 14 E 11 YHL035C VMR1 putative ATP-binding cassette multidrug transporter VMR1 Vacuolar membrane protein; involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions; VMR1 has a paralog, YBT1, that arose from the whole genome duplication response to chemical hydrolase activity, transmembrane transporter activity, ATPase activity vacuole, ribosome, mitochondrion, membrane, cytoplasm 928 BY4741 08_1 14 E 12 YHL034C SBP1 SSB1|SSBR1 Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; binds to mRNAs under glucose starvation stress, most often in the 5' UTR; found associated with small nucleolar RNAs snR10 and snR11; SBP1 has a paralog, RNP1, that arose from the whole genome duplication regulation of translation RNA binding, mRNA binding nucleus, nucleolus, cytoplasm 929 BY4741 08_1 14 F 1 YHL033C RPL8A ribosomal 60S subunit protein L8A|L8e|rp6|YL5|L8A|L4A|MAK7 Ribosomal 60S subunit protein L8A; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal large subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 930 BY4741 08_1 14 F 2 YHL032C GUT1 glycerol kinase Glycerol kinase; converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p carbohydrate metabolic process kinase activity, transferase activity mitochondrion, cytoplasm 931 BY4741 08_1 14 F 3 YHL031C GOS1 - v-SNARE protein involved in Golgi transport; homolog of the mammalian protein GOS-28/GS28 membrane fusion, Golgi vesicle transport, organelle fusion, vesicle organization other membrane, endomembrane system, cytoplasm, Golgi apparatus 932 BY4741 08_1 14 F 4 YHL030W ECM29 - Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; inhibits proteasomal ATPase activity; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress protein complex biogenesis structural molecule activity nucleus, cytoplasm 933 BY4741 08_1 "Cell wall biogenesis & architecture -- growth on -met, no growth on -lys, no growth on drop-in media -- APPEARS ALPHA " 14 F 5 YHL029C OCA5 - Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts biological_process molecular_function cytoplasm 934 BY4741 08_1 14 F 6 YHL028W WSC4 YFW1|YHC8 Endoplasmic reticulum (ER) membrane protein; involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 response to heat, protein targeting protein transporter activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 935 BY4741 08_1 14 F 7 YHL027W RIM101 alkaline-responsive transcriptional regulator RIM101|RIM1 Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC mitotic cell cycle, meiotic cell cycle, response to chemical, organelle fission, sporulation, cytokinesis, cell wall organization or biogenesis, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 936 BY4741 08_1 14 F 8 YHL026C - - Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) biological_process molecular_function cellular_component 937 BY4741 08_1 14 F 9 YHL023C NPR3 RMD11 Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of SEAC, a coatomer-related complex that associates dynamically with the vacuole; Npr3p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and leading to inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; null mutant has meiotic defects; human NPRL3 homolog vacuole organization, meiotic cell cycle, organelle fission, regulation of organelle organization, organelle assembly, response to starvation, pseudohyphal growth, signaling molecular_function vacuole, membrane, cytoplasm 940 BY4741 08_1 14 F 10 YHL022C SPO11 - Meiosis-specific protein that initiates meiotic recombination; initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation meiotic cell cycle, organelle fission nuclease activity, hydrolase activity nucleus, chromosome 941 BY4741 08_1 14 F 11 YHL021C AIM17 FMP12 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss mitochondrion organization molecular_function mitochondrion, cytoplasm 942 BY4741 08_1 14 F 12 YHL020C OPI1 transcriptional regulator OPI1 Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance; null exhibits disrupted mitochondrial metabolism and low cardiolipin content, strongly correlated with overproduction of inositol response to chemical, lipid metabolic process, transcription from RNA polymerase II promoter, signaling transcription factor activity, protein binding nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 943 BY4741 08_1 14 G 1 YHL019C APM2 - Protein of unknown function; homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Golgi vesicle transport other membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 944 BY4741 08_1 14 G 2 YHL017W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication biological_process molecular_function membrane, cytoplasmic membrane-bounded vesicle, cytoplasm 946 BY4741 08_1 14 G 3 YHL016C DUR3 - Plasma membrane transporter for both urea and polyamines; expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway ion transport transmembrane transporter activity membrane, plasma membrane 947 BY4741 08_1 14 G 4 YHL014C YLF2 YLF1 Protein of unknown function; has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process ion binding mitochondrion, cytoplasm 949 BY4741 08_1 14 G 5 YHL013C OTU2 - Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress biological_process molecular_function ribosome, cytoplasm 950 BY4741 08_1 14 G 6 YHL012W - UTP--glucose-1-phosphate uridylyltransferase Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication biological_process molecular_function cellular_component 951 BY4741 08_1 14 G 7 YHL010C ETP1 BRP2 Putative protein of unknown function required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences response to chemical ubiquitin-like protein binding cellular_component 953 BY4741 08_1 14 G 8 YHL009C YAP3 - Basic leucine zipper (bZIP) transcription factor transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 954 BY4741 08_1 14 G 9 YHL008C - - Putative protein of unknown function; may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole ion transport other membrane, vacuole, cytoplasm 955 BY4741 08_1 14 G 10 YHL007C STE20 mitogen-activated protein kinase kinase kinase kinase STE20 Cdc42p-activated signal transducing kinase; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p; member of the PAK (p21-activated kinase) family response to heat, response to chemical, organelle fission, transmembrane transport, regulation of transport, lipid transport, protein phosphorylation, regulation of organelle organization, pseudohyphal growth, mitotic cell cycle, vacuole organization, conjugation, transcription from RNA polymerase II promoter, response to osmotic stress, invasive growth in response to glucose limitation, cell budding, organelle assembly, cytokinesis, regulation of protein modification process, organelle inheritance, regulation of cell cycle, signaling RNA binding, signal transducer activity, mRNA binding, kinase activity, transferase activity nucleus, plasma membrane, cytoplasm, membrane, site of polarized growth 956 BY4741 08_1 Sterile [expected phenotype] 14 G 11 YHL006C SHU1 - Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Csm2, Shu2, and promotes error-free DNA repair, mediates inhibition of Srs2p function; essential for promoting the establishment of homolog bias during meiotic homologous recombination; promotes both crossover (CO) and non-crossover (NCO) pathways of meiotic recombination and formation of Rad51p filaments DNA repair, DNA recombination, cellular response to DNA damage stimulus molecular_function nucleus, nucleolus, chromosome 957 BY4741 08_1 14 G 12 YHL005C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W biological_process molecular_function cellular_component 959 BY4741 08_1 "Hypothetical protein -- growth on -met, no growth on -lys, no growth on drop-in media, petite -- APPEARS ALPHA" 14 H 1 YHL003C LAG1 sphingosine N-acyltransferase LAG1 Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enahnces invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication lipid metabolic process transferase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 960 BY4741 08_1 "Longevity assurance protein -- growth on -met, no growth on -lys, no growth on drop-in media -- APPEARS ALPHA" 14 H 3 YHR001W-A QCR10 ubiquinol--cytochrome-c reductase subunit 10 Subunit of the ubiqunol-cytochrome c oxidoreductase complex; this complex comprises part of the mitochondrial respiratory chain; members include Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 964 BY4741 08_1 14 H 4 YHR011W DIA4 putative serine--tRNA ligase DIA4 Probable mitochondrial seryl-tRNA synthetase; mutant displays increased invasive and pseudohyphal growth mitochondrial translation, invasive growth in response to glucose limitation, mitochondrion organization, cellular amino acid metabolic process, tRNA aminoacylation for protein translation, pseudohyphal growth ligase activity mitochondrion, cytoplasm 974 BY4741 08_1 14 H 5 YHR012W VPS29 retromer subunit VPS29|VPT6|PEP11 Subunit of the membrane-associated retromer complex; endosomal protein; essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval endosomal transport protein transporter activity membrane, endomembrane system, cytoplasm 975 BY4741 08_1 14 H 6 YHR013C ARD1 peptide alpha-N-acetyltransferase complex A subunit ARD1|NAA10 Subunit of protein N-terminal acetyltransferase NatA; NatA comprises Nat1p, Ard1p, Nat5p; acetylates many proteins to influence telomeric silencing, cell cycle, heat-shock resistance, mating, sporulation, early stages of mitophagy; protein abundance increases under DNA replication stress; mutations in human homolog X-linked NAA10 lead to Ogden syndrome (S37P) and intellectual disability (R116W); expression of human NAA10 and NAA15 can complement ard1 nat1 double mutant peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity ribosome, cytoplasm 976 BY4741 08_1 14 H 7 YHR014W SPO13 - Meiotic regulator; involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II; anaphase-promoting complex (APC) substrate that is degraded during anaphase I; expressed only in meiotic cells meiotic cell cycle, organelle fission, regulation of organelle organization, chromosome segregation, regulation of cell cycle molecular_function nucleus, cytoskeleton, chromosome 977 BY4741 08_1 14 H 8 YHR015W MIP6 - Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication nucleobase-containing compound transport, nuclear transport RNA binding cellular_component 978 BY4741 08_1 14 H 9 YHR018C ARG4 argininosuccinate lyase ARG4 Argininosuccinate lyase; catalyzes the final step in the arginine biosynthesis pathway cellular amino acid metabolic process lyase activity cytoplasm 981 BY4741 08_1 14 H 10 YHR022C - - Putative protein of unknown function; YHR022C is not an essential gene biological_process molecular_function cellular_component 985 BY4741 08_1 14 H 11 YHR029C YHI9 - Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production response to chemical, signaling molecular_function cellular_component 992 BY4741 08_1 14 H 12 YHR030C SLT2 mitogen-activated serine/threonine-protein kinase SLT2|LYT2|SLK2|MPK1|BYC2 Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, cell cycle progression, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to phagophore assembly site (PAS); plays a role in adaptive response of cells to cold; regulated by the PKC1-mediated signaling pathway; SLT2 has a paralog, KDX1, that arose from the whole genome duplication mitotic cell cycle, response to chemical, regulation of transport, peroxisome organization, mRNA processing, cell wall organization or biogenesis, protein targeting, RNA splicing, protein phosphorylation, cytokinesis, nuclear transport, signaling signal transducer activity, kinase activity, transferase activity nucleus, mitochondrion, cellular bud, site of polarized growth, cytoplasm 993 BY4741 08_1 15 A 1 YHR031C RRM3 RTT104 DNA helicase involved in rDNA replication and Ty1 transposition; binds to and suppresses DNA damage at G4 motifs in vivo; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p DNA replication, mitochondrion organization, regulation of DNA metabolic process hydrolase activity, ATPase activity, helicase activity nucleus, chromosome 994 BY4741 08_1 15 A 2 YHR034C PIH1 NOP17 Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II protein folding, rRNA processing molecular_function nucleus, cytoplasm 997 BY4741 08_1 15 A 3 YHR035W NEL1 - Activator of Sar1p GTPase activity; paralog of Sec23 but does not associate with the COPII components; not an essential gene biological_process enzyme regulator activity cytoplasm 998 BY4741 08_1 15 A 4 YHR037W PUT2 1-pyrroline-5-carboxylate dehydrogenase Delta-1-pyrroline-5-carboxylate dehydrogenase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of human homolog ALDH4A1 causes type II hyperprolinemia (HPII), an autosomal recessive inborn error of metabolism; human homolog ALDH4A1 can complement yeast null mutant cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 1000 BY4741 08_1 15 A 5 YHR038W RRF1 FIL1|KIM4 Mitochondrial ribosome recycling factor; essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria mitochondrial translation, mitochondrion organization RNA binding, translation factor activity, RNA binding mitochondrion, cytoplasm 1001 BY4741 08_1 15 A 6 YHR039C MSC7 meiotic recombination directing protein Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids meiotic cell cycle, organelle fission, DNA recombination molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1002 BY4741 08_1 15 A 7 YHR043C DOG2 - 2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed; DOG2 has a paralog, DOG1, that arose from a single-locus duplication; the last half of DOG1 and DOG2 are subject to gene conversions among S. cerevisiae, S. paradoxus, and S. mikatae carbohydrate metabolic process phosphatase activity, hydrolase activity nucleus, cytoplasm 1006 BY4741 08_1 15 A 8 YHR044C DOG1 - 2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed; DOG1 has a paralog, DOG2, that arose from a single-locus duplication; the last half of DOG1 and DOG2 are subject to gene conversions among S. cerevisiae, S. paradoxus, and S. mikatae carbohydrate metabolic process phosphatase activity, hydrolase activity cellular_component 1007 BY4741 08_1 15 A 9 YHR046C INM1 inositol monophosphate 1-phosphatase INM1 Inositol monophosphatase; involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate carbohydrate metabolic process phosphatase activity, hydrolase activity nucleus, cytoplasm 1873 BY4741 08_2 15 A 10 YHR047C AAP1 AAP1' Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication carbohydrate metabolic process, generation of precursor metabolites and energy peptidase activity, hydrolase activity nucleus, cytoplasm 1874 BY4741 08_2 15 A 11 YHR048W YHK8 - Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles response to chemical molecular_function membrane, plasma membrane 1875 BY4741 08_2 15 A 12 YHR049W FSH1 putative serine hydrolase Putative serine hydrolase; localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 biological_process molecular_function nucleus, cytoplasm 1876 BY4741 08_2 15 B 1 YHR049C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1877 BY4741 08_2 15 B 2 YHR050W SMF2 divalent metal ion transporter SMF2 Divalent metal ion transporter involved in manganese homeostasis; has broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins ion transport, cellular ion homeostasis transmembrane transporter activity endomembrane system, mitochondrion, membrane, Golgi apparatus, cytoplasm 1878 BY4741 08_2 15 B 4 YHR051W COX6 cytochrome c oxidase subunit VI Subunit VI of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1879 BY4741 08_2 APPEARS TO BE ALPHA 15 B 5 YHR057C CPR2 peptidylprolyl isomerase CPR2|CYP2 Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; CPR2 has a paralog, CPR5, that arose from the whole genome duplication; suppresses toxicity of slow-folding human Z-type alpha1-antitrypsin variant associated with liver cirrhosis and emphysema biological_process isomerase activity cellular_component 1885 BY4741 08_2 15 B 6 YHR060W VMA22 VPH6|CEV1 Protein that is required for vacuolar H+-ATPase (V-ATPase) function; peripheral membrane protein; not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) cellular ion homeostasis, protein complex biogenesis unfolded protein binding membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1888 BY4741 08_2 15 B 7 YHR061C GIC1 - Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress; GIC1 has a paralog, GIC2, that arose from the whole genome duplication mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, regulation of cell cycle enzyme regulator activity, enzyme binding nucleus, cellular bud, site of polarized growth, cytoplasm 1889 BY4741 08_2 15 B 8 YHR066W SSF1 - Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication ribosome assembly, rRNA processing, conjugation, organelle assembly, ribosomal large subunit biogenesis RNA binding, rRNA binding nucleus, nucleolus 1894 BY4741 08_2 15 B 9 YHR073W OSH3 oxysterol-binding protein related protein OSH3 Member of an oxysterol-binding protein family; this family has seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; contains FFAT motif; interacts with ER anchor Scs2p at patches at the plasma membrane; regulated by sterol binding protein dephosphorylation, organelle fusion, lipid transport, conjugation, invasive growth in response to glucose limitation, regulation of protein modification process, membrane invagination, exocytosis, pseudohyphal growth, endocytosis, nucleus organization ion binding, lipid binding cell cortex, plasma membrane, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 1901 BY4741 08_2 15 B 10 YHR075C PPE1 carboxylesterase-mitochondrial 37S ribosomal protein YmS2|YmS2|MRPS2 Protein with carboxyl methyl esterase activity; may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein mitochondrial translation, mitochondrion organization structural constituent of ribosome, hydrolase activity, structural molecule activity ribosome, mitochondrion, cytoplasm 1903 BY4741 08_2 15 B 11 YHR076W PTC7 type 2C protein phosphatase PTC7 Type 2C serine/threonine protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope; activates coenzyme Q6 biosynthesis by dephosphorylation of demethoxy-Q6 hydroxylase Coq7p protein dephosphorylation phosphatase activity, hydrolase activity nucleus, endomembrane system, mitochondrion, cytoplasm 1904 BY4741 08_2 15 B 12 YHR077C NMD2 SUP111|UPF2|SUA1|IFS1 Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance RNA catabolic process, DNA recombination molecular_function cytoplasm 1905 BY4741 08_2 15 C 1 YHR078W - - High osmolarity-regulated gene of unknown function biological_process molecular_function membrane 1906 BY4741 08_2 15 C 2 YHR079C IRE1 bifunctional endoribonuclease/protein kinase IRE1|ERN1 Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; role in homeostatic adaptation to ER stress; Kar2p binds inactive Ire1p and releases from it upon ER stress response to chemical, cell wall organization or biogenesis, carbohydrate metabolic process, protein phosphorylation, protein complex biogenesis, signaling hydrolase activity, unfolded protein binding, kinase activity, transferase activity, nuclease activity nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 1907 BY4741 08_2 15 C 3 YHR080C LAM4 LTC3 Sterol-binding protein that localizes to puncta in the cortical ER; sterol binding occurs via two StART-like domains; one of six StART-like domain-containing proteins in yeast that may be involved in intracellular sterol transfer between membranes; conserved across eukaryotes; has both GRAM and StART-like (VASt) domains; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies lipid transport lipid binding cell cortex, endomembrane system, ribosome, mitochondrion, endoplasmic reticulum, cytoplasm 1908 BY4741 08_2 15 C 4 YHR081W LRP1 RRP47|YC1D Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; forms a stable heterodimer with Rrp6p and regulates its exonucleolytic activity; rapidly degraded by the proteasome in the absence of Rrp6p; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination RNA catabolic process, snoRNA processing, rRNA processing RNA binding, DNA binding, enzyme regulator activity nucleus 1909 BY4741 08_2 15 C 5 YHR082C KSP1 putative serine/threonine protein kinase KSP1 Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress protein phosphorylation, pseudohyphal growth, signaling RNA binding, kinase activity, transferase activity, mRNA binding nucleus, cytoplasm 1910 BY4741 08_2 15 C 6 YHR086W NAM8 MUD15|MRE2 RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function RNA splicing, mRNA processing RNA binding, mRNA binding nucleus, cytoplasm 1914 BY4741 08_2 15 C 7 YHR087W RTC3 HGI1 Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress other molecular_function nucleus, cytoplasm 1915 BY4741 08_2 15 C 8 YHR091C MSR1 arginine--tRNA ligase MSR1 Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6; MSR1 has a paralog, YDR341C, that arose from the whole genome duplication mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 1919 BY4741 08_2 15 C 9 YHR092C HXT4 hexose transporter HXT4|RAG1|LGT1 High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication carbohydrate transport transmembrane transporter activity membrane, plasma membrane 1920 BY4741 08_2 15 C 10 YHR093W AHT1 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region biological_process molecular_function cellular_component 1921 BY4741 08_2 15 C 11 YHR094C HXT1 hexose transporter HXT1|HOR4 Low-affinity glucose transporter of the major facilitator superfamily; expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication carbohydrate transport transmembrane transporter activity membrane, plasma membrane 1922 BY4741 08_2 15 C 12 YHR095W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1923 BY4741 08_2 15 D 1 YHR096C HXT5 hexose transporter HXT5 Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication carbohydrate transport transmembrane transporter activity membrane, plasma membrane 1924 BY4741 08_2 15 D 2 YHR097C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YHR097C has a paralog, PAL1, that arose from the whole genome duplication biological_process molecular_function nucleus, cytoplasm 1925 BY4741 08_2 15 D 3 YHR100C GEP4 - Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase); dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT carbohydrate metabolic process, lipid metabolic process phosphatase activity, hydrolase activity mitochondrion, cytoplasm 1928 BY4741 08_2 15 D 4 YHR103W SBE22 - Protein involved in bud growth; involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; SBE22 has a paralog, SBE2, that arose from the whole genome duplication cell wall organization or biogenesis molecular_function cytoplasm 1931 BY4741 08_2 15 D 5 YHR104W GRE3 trifunctional aldehyde reductase/xylose reductase/glucose 1-dehydrogenase (NADP(+)) Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress carbohydrate metabolic process, response to chemical, response to oxidative stress, response to osmotic stress RNA binding, oxidoreductase activity, mRNA binding nucleus, cytoplasm 1932 BY4741 08_2 15 D 6 YHR105W YPT35 - Endosomal protein of unknown function; contains a phox (PX) homology domain; binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport biological_process ion binding, lipid binding endomembrane system, cytoplasm 1933 BY4741 08_2 15 D 7 YHR106W TRR2 thioredoxin-disulfide reductase TRR2 Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication response to chemical, response to oxidative stress oxidoreductase activity mitochondrion, cytoplasm 1934 BY4741 08_2 15 D 8 YHR108W GGA2 - Protein that regulates Arf1p, Arf2p to facilitate Golgi trafficking; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin; GGA2 has a paralog, GGA1, that arose from the whole genome duplication protein targeting, proteolysis involved in cellular protein catabolic process, Golgi vesicle transport, endosomal transport lipid binding, ubiquitin-like protein binding, ion binding endomembrane system, Golgi apparatus, cytoplasm 1936 BY4741 08_2 15 D 9 YHR109W CTM1 - Cytochrome c lysine methyltransferase; trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth peptidyl-amino acid modification, protein alkylation transferase activity, methyltransferase activity cytoplasm 1937 BY4741 08_2 15 D 10 YHR110W ERP5 - Protein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport other molecular_function membrane 1938 BY4741 08_2 15 D 11 YHR111W UBA4 YHR1 E1-like protein that activates Urm1p before urmylation; also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p response to chemical, invasive growth in response to glucose limitation, cell budding, response to oxidative stress, tRNA processing, protein modification by small protein conjugation or removal, pseudohyphal growth, RNA modification transferase activity, nucleotidyltransferase activity, ligase activity cytoplasm 1939 BY4741 08_2 15 D 12 YHR112C - putative cystathionine beta-lyase Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway other lyase activity nucleus, cytoplasm 1940 BY4741 08_2 15 E 1 YHR113W APE4 aspartyl aminopeptidase Cytoplasmic aspartyl aminopeptidase with possible vacuole function; Cvt pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family other peptidase activity, hydrolase activity vacuole, ribosome, cytoplasm 1941 BY4741 08_2 15 E 2 YHR114W BZZ1 LSB7 SH3 domain protein implicated in regulating actin polymerization; able to recruit actin polymerization machinery through its SH3 domains; colocalizes with cortical actin patches and Las17p; interacts with type I myosins cytoskeleton organization, regulation of organelle organization, protein complex biogenesis, response to osmotic stress, endocytosis enzyme regulator activity cell cortex, cytoskeleton, membrane, site of polarized growth, cytoplasm, plasma membrane 1942 BY4741 08_2 15 E 3 YHR115C DMA1 ubiquitin-conjugating protein DMA1|CHF1 Ubiquitin-protein ligase (E3); controls septin dynamics, spindle position checkpoint (SPOC) with ligase Dma2p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8, similar to human Chfr; contains FHA, RING fingers; DMA1 has a paralog, DMA2, that arose from the whole genome duplication mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, organelle assembly, cytokinesis, proteolysis involved in cellular protein catabolic process, protein complex biogenesis, chromosome segregation, protein modification by small protein conjugation or removal, regulation of cell cycle transferase activity cytoplasm 1943 BY4741 08_2 15 E 4 YHR116W COX23 - Protein that functions in mitochondrial copper homeostasis; mitochondrial intermembrane space protein; essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs mitochondrion organization, protein complex biogenesis molecular_function mitochondrion, cytoplasm 1944 BY4741 08_2 15 E 5 YHR117W TOM71 protein channel TOM71|TOM72 Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication protein targeting, mitochondrion organization protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1945 BY4741 08_2 15 E 6 YHR120W MSH1 mismatch repair ATPase MSH1 DNA-binding protein of the mitochondria; involved in repair of mitochondrial DNA; has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis DNA repair, mitochondrion organization, cellular response to DNA damage stimulus hydrolase activity, DNA binding, ATPase activity mitochondrion, cytoplasm 1948 BY4741 08_2 15 E 7 YHR121W LSM12 - Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress other RNA binding nucleus, ribosome, cytoplasm 1949 BY4741 08_2 15 E 8 YHR123W EPT1 bifunctional diacylglycerol cholinephosphotransferase/ethanolaminephosphotransferase sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication lipid metabolic process transferase activity membrane, endomembrane system, cytoplasm, Golgi apparatus 1951 BY4741 08_2 15 E 9 YHR124W NDT80 transcription factor NDT80 Meiosis-specific transcription factor; required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) meiotic cell cycle, organelle fission, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, chromosome 1952 BY4741 08_2 15 E 10 YHR125W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1953 BY4741 08_2 15 E 11 YHR126C ANS1 - Putative GPI protein; transcription dependent upon Azf1p biological_process molecular_function cellular_component 1954 BY4741 08_2 15 E 12 YHR129C ARP1 actin-related protein 1|ACT5 Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; forms actin-like short filament composed of 9 or 10 Arp1p monomers; putative ortholog of mammalian centractin mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, proteolysis involved in cellular protein catabolic process, chromosome segregation, regulation of cell cycle structural molecule activity cell cortex, cytoskeleton, microtubule organizing center, cytoplasm 1957 BY4741 08_2 15 F 1 YHR130C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1958 BY4741 08_2 15 F 2 YHR132C ECM14 - Putative metalloprotease with similarity to zinc carboxypeptidases; required for normal cell wall assembly other peptidase activity, hydrolase activity vacuole, cytoplasm 1960 BY4741 08_2 15 F 3 YHR133C NSG1 - Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; forms foci at the nuclear periphery upon DNA replication stress; relocalizes to the cytosol in response to hypoxia; homolog of mammalian INSIG proteins; NSG1 has a paralog, NSG2, that arose from the whole genome duplication lipid metabolic process unfolded protein binding nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1961 BY4741 08_2 15 F 4 YHR134W WSS1 metalloendopeptidase WSS1 Metalloprotease involved in DNA repair, removes DNA-protein crosslinks at stalled replication forks during replication of damaged DNA; sumoylated protein localizing to the nuclear periphery of mother cells; localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; activated by DNA binding peptidyl-amino acid modification, DNA repair, protein modification by small protein conjugation or removal, cellular response to DNA damage stimulus peptidase activity, hydrolase activity nucleus, endomembrane system 1962 BY4741 08_2 15 F 5 YHR135C YCK1 serine/threonine protein kinase YCK1|CKI2 Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; YCK1 has a paralog, YCK2, that arose from the whole genome duplication response to chemical, protein phosphorylation, cell morphogenesis, endocytosis kinase activity, transferase activity endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm, plasma membrane 1963 BY4741 08_2 15 F 6 YHR136C SPL2 - Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation by targeting Pho87p to the vacuole; upstream region harbors putative hypoxia response element (HRE) cluster; overproduction suppresses a plc1 null mutation; promoter shows an increase in Snf2p occupancy after heat shock; GFP-fusion protein localizes to the cytoplasm protein targeting enzyme regulator activity cytoplasm 1964 BY4741 08_2 15 F 7 YHR137W ARO9 aromatic-amino-acid:2-oxoglutarate transaminase Aromatic aminotransferase II; catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism cellular amino acid metabolic process transferase activity nucleus, cytoplasm 1965 BY4741 08_2 15 F 8 YHR138C - - Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress vacuole organization, membrane fusion, organelle fusion enzyme regulator activity cellular_component 1966 BY4741 08_2 15 F 9 YHR139C SPS100 - Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin; SPS100 has a paralog, YGP1, that arose from the whole genome duplication meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function cell wall 1967 BY4741 08_2 15 F 10 YHR139C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1968 BY4741 08_2 15 F 11 YHR142W CHS7 - Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress carbohydrate metabolic process, Golgi vesicle transport, protein folding, cell wall organization or biogenesis unfolded protein binding endomembrane system, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm 2835 BY4741 08_3 15 F 12 YHR143W DSE2 - Daughter cell-specific secreted protein with similarity to glucanases; degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP pseudohyphal growth hydrolase activity, hydrolase activity, acting on glycosyl bonds cell wall, extracellular region 2836 BY4741 08_3 15 G 1 YHR147C MRPL6 mitochondrial 54S ribosomal protein YmL16|YmL16 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 2841 BY4741 08_3 15 G 2 YHR150W PEX28 - Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p peroxisome organization molecular_function peroxisome, membrane, cytoplasm 2844 BY4741 08_3 15 G 3 YHR151C MTC6 - Protein of unknown function; mtc6 is synthetically sick with cdc13-1 biological_process molecular_function cellular_component 2845 BY4741 08_3 15 G 4 YHR152W SPO12 SDB21 Nucleolar protein of unknown function; positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis; SPO12 has a paralog, BNS1, that arose from the whole genome duplication mitotic cell cycle, meiotic cell cycle, organelle fission, regulation of organelle organization, regulation of cell cycle molecular_function nucleus, nucleolus 2846 BY4741 08_3 15 G 5 YHR153C SPO16 - Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation peptidyl-amino acid modification, meiotic cell cycle, organelle fission, sporulation, regulation of organelle organization, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, DNA recombination, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, chromosome 2847 BY4741 08_3 15 G 6 YHR154W RTT107 ESC4 Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress DNA repair, cellular response to DNA damage stimulus, transposition molecular_function nucleus 2848 BY4741 08_3 15 G 7 YHR155W LAM1 YSP1 Putative sterol transfer protein; localizes to puncta in the cortical ER; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; has both GRAM and StART-like (VASt) domains lipid transport other cell cortex, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm 2849 BY4741 08_3 15 G 8 YHR156C LIN1 U5 snRNP complex subunit LIN1|SNU40 Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication biological_process other nucleus, chromosome 2850 BY4741 08_3 15 G 9 YHR157W REC104 - Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination meiotic cell cycle, organelle fission, DNA recombination molecular_function nucleus, chromosome 2851 BY4741 08_3 15 G 10 YHR158C KEL1 - Protein required for proper cell fusion and cell morphology; forms a complex with Bud14p and Kel2p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate mitotic cell cycle, organelle fission, cell morphogenesis, cytoskeleton organization, regulation of organelle organization, cytokinesis, regulation of transport, conjugation, regulation of cell cycle molecular_function cell cortex, cellular bud, membrane, site of polarized growth, cytoplasm 2852 BY4741 08_3 15 G 11 YHR159W TDA11 - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function cytoplasm 2853 BY4741 08_3 15 G 12 YHR160C PEX18 - Peroxin; required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p; primarily responsible for peroxisomal import during growth on oleate, and expression is induced during oleate growth protein targeting, transmembrane transport, peroxisome organization other peroxisome, cytoplasm 2854 BY4741 08_3 15 H 1 YHR161C YAP1801 - Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication endocytosis other membrane, cell cortex, cytoskeleton, cytoplasm 2855 BY4741 08_3 15 H 3 YHR163W SOL3 6-phosphogluconolactonase SOL3 6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication cofactor metabolic process, nucleobase-containing small molecule metabolic process hydrolase activity nucleus, cytoplasm 2857 BY4741 08_3 15 H 4 YHR167W THP2 - Subunit of the THO and TREX complexes; THO connects transcription elongation and mitotic recombination, and TREX is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance telomere organization, nucleobase-containing compound transport, DNA recombination, nuclear transport, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation other nucleus 2861 BY4741 08_3 15 H 5 YHR176W FMO1 - Flavin-containing monooxygenase; localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins protein folding oxidoreductase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 2870 BY4741 08_3 15 H 6 YHR177W - GON3 Putative transcription factor containing a WOPR domain; binds DNA in vitro; similar to C. albicans Wor1p transcription factor that regulates white-opaque switching; overexpression causes a cell cycle delay or arrest transcription from RNA polymerase II promoter DNA binding nucleus 2871 BY4741 08_3 15 H 7 YHR178W STB5 - Transcription factor; involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro response to chemical, transcription from RNA polymerase II promoter, response to oxidative stress nucleic acid binding transcription factor activity, DNA binding nucleus 2872 BY4741 08_3 15 H 8 YHR179W OYE2 - Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress other oxidoreductase activity nucleus, mitochondrion, cytoplasm 2873 BY4741 08_3 15 H 9 YHR182W - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress biological_process molecular_function cellular bud, site of polarized growth, cytoplasm 2876 BY4741 08_3 15 H 10 YHR183W GND1 phosphogluconate dehydrogenase (decarboxylating) GND1 6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication response to chemical, cofactor metabolic process, nucleobase-containing small molecule metabolic process, response to oxidative stress oxidoreductase activity mitochondrion, cytoplasm 2877 BY4741 08_3 15 H 11 YHR184W SSP1 SPO3 Protein involved in the control of meiotic nuclear division; also involved in the coordination of meiosis with spore formation; transcription is induced midway through meiosis meiotic cell cycle, sporulation, cell wall organization or biogenesis, protein complex biogenesis molecular_function membrane 2878 BY4741 08_3 15 H 12 YHR189W PTH1 aminoacyl-tRNA hydrolase|PTH One of two mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium; see also PTH2 mitochondrial translation, mitochondrion organization hydrolase activity mitochondrion, cytoplasm 2883 BY4741 08_3 16 A 1 YHR195W NVJ1 VAB36 Nuclear envelope protein; anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) membrane invagination other nucleus, endomembrane system 2889 BY4741 08_3 16 A 2 YHR198C AIM18 FMP22 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 2892 BY4741 08_3 16 A 3 YHR199C AIM46 FMP34 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 2893 BY4741 08_3 16 A 4 YHR200W RPN10 proteasome regulatory particle base subunit RPN10|SUN1|MCB1 Non-ATPase base subunit of the 19S RP of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the regulatory particle (RP); binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein proteolysis involved in cellular protein catabolic process ubiquitin-like protein binding, structural molecule activity other 2894 BY4741 08_3 16 A 5 YHR202W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization biological_process molecular_function vacuole, cytoplasm 2896 BY4741 08_3 16 A 6 YHR203C RPS4B ribosomal 40S subunit protein S4B|S4e|rp5|YS6|S7B|S4B Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 2897 BY4741 08_3 16 A 7 YHR204W MNL1 alpha-1,2-mannosidase MNL1|HTM1 Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation; human homolog EDEM1 can complement yeast null mutant response to chemical, proteolysis involved in cellular protein catabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds endomembrane system, endoplasmic reticulum, cytoplasm 2898 BY4741 08_3 16 A 8 YHR206W SKN7 kinase-regulated stress-responsive transcription factor SKN7|POS9|BRY1 Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; relocalizes to the cytosol in response to hypoxia; SKN7 has a paralog, HMS2, that arose from the whole genome duplication response to chemical, response to oxidative stress, transcription from RNA polymerase II promoter, response to osmotic stress nucleic acid binding transcription factor activity, DNA binding, signal transducer activity nucleus, cytoplasm 2900 BY4741 08_3 16 A 9 YHR207C SET5 - Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus peptidyl-amino acid modification, protein alkylation, chromatin organization, histone modification transferase activity, methyltransferase activity nucleus, chromosome, cytoplasm 2901 BY4741 08_3 16 A 10 YHR209W CRG1 - S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin other RNA binding, transferase activity, methyltransferase activity, mRNA binding cellular_component 2903 BY4741 08_3 16 A 11 YHR210C - aldose 1-epimerase superfamily protein Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions biological_process molecular_function cellular_component 2904 BY4741 08_3 16 A 12 YCL001W RER1 protein retreival receptor Protein involved in retention of membrane proteins; including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Golgi vesicle transport molecular_function membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, vacuole, cytoplasm, Golgi apparatus 3409 BY4741 03_1 16 B 1 YCL002C - - Putative protein of unknown function; YCL002C is not an essential gene biological_process molecular_function membrane 3410 BY4741 03_1 16 B 2 YCL005W LDB16 - Protein involved in lipid droplet (LD) assembly; forms a complex with Sei1p at ER-LD contact sites, and stabilizes the sites of contact; null mutants have decreased net negative cell surface charge; integral ER membrane protein; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; null mutant can be complemented by the human seipin, BSCL2 biological_process molecular_function mitochondrion, cytoplasm 3413 BY4741 03_1 16 B 3 YMR118C SHH3 protein SHH3 Putative mitochondrial inner membrane protein of unknown function; although similar to paralogous Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase; SHH3 has a paralog, SDH3, that arose from the whole genome duplication biological_process molecular_function membrane 7121 BY4741 00_15 16 B 5 YCL008C STP22 ubiquitin-binding ESCRT-I subunit protein STP22|AGS1|VPL15|VPS23 Component of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; prevents polyubiquitination of the arrestin-related protein Rim8p, thereby directing its monoubiquitination by Rsp5p; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype; protein targeting, proteolysis involved in cellular protein catabolic process, regulation of protein modification process, endosomal transport, protein modification by small protein conjugation or removal ubiquitin-like protein binding endomembrane system, membrane, cytoplasm, plasma membrane 3416 BY4741 03_1 16 B 6 YCL009C ILV6 acetolactate synthase regulatory subunit Regulatory subunit of acetolactate synthase; acetolactate synthase catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria cellular amino acid metabolic process transferase activity, enzyme regulator activity mitochondrion, cytoplasm 3417 BY4741 03_1 16 B 7 YCL010C SGF29 - Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation peptidyl-amino acid modification, protein acylation, chromatin organization, transcription from RNA polymerase II promoter, histone modification histone binding, transcription factor activity, protein binding nucleus 3418 BY4741 03_1 16 B 8 YCL011C GBP2 RLF6 Poly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; relocalizes to the cytosol in response to hypoxia; GBP2 has a paralog, HRB1, that arose from the whole genome duplication telomere organization, nucleobase-containing compound transport, RNA catabolic process, nuclear transport RNA binding, DNA binding, mRNA binding nucleus, cytoplasm 3419 BY4741 03_1 16 B 9 YCL012W nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - 3420 BY4741 03_1 16 B 10 YCL013W - - - - - - 3421 BY4741 03_1 16 B 11 YCL014W BUD3 YCL012W Guanine nucleotide exchange factor (GEF) for Cdc42p; activates Cdc42p in early G1, accounting for the first stage of biphasic activation, with Cdc24p accounting for the second stage in late G1; involved in the Cdc42p-mediated assembly of the axial landmark that dictates the site for the next round of budding, resulting in the axial budding pattern observed in haploids; localizes with septins to the bud neck contractile ring in mitosis mitotic cell cycle, cell budding, cytokinesis, conjugation guanyl-nucleotide exchange factor activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 3422 BY4741 03_1 16 B 12 YCL016C DCC1 - Subunit of a complex with Ctf8p and Ctf18p; shares some components with Replication Factor C; required for sister chromatid cohesion and telomere length maintenance mitotic cell cycle, organelle fission, cellular response to DNA damage stimulus, chromosome segregation molecular_function chromosome 3423 BY4741 03_1 16 C 1 YCL023C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 biological_process molecular_function cellular_component 3430 BY4741 03_1 16 C 2 YCL024W KCC4 - Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication mitotic cell cycle, cytoskeleton organization, cell budding, protein phosphorylation, organelle assembly, protein complex biogenesis, regulation of cell cycle ion binding, kinase activity, transferase activity, lipid binding cell cortex, cytoskeleton, plasma membrane, cellular bud, membrane, site of polarized growth, cytoplasm 3431 BY4741 03_1 16 C 3 YCL025C AGP1 amino acid transporter AGP1|YCC5 Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication transmembrane transport, ion transport, amino acid transport transmembrane transporter activity membrane, plasma membrane 3432 BY4741 03_1 16 C 4 YCL026C membrane, plasma membrane membrane, plasma membrane membrane, plasma membrane - - - 3433 BY4741 03_1 16 C 5 YCL027W FUS1 - Membrane protein localized to the shmoo tip; required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate response to chemical, cell morphogenesis, conjugation molecular_function membrane, site of polarized growth, plasma membrane 3434 BY4741 03_1 16 C 6 YCL028W RNQ1 prion domain-containing protein RNQ1|[PIN(+)] [PIN(+)] prion; an infectious protein conformation that is generally an ordered protein aggregate biological_process molecular_function cytoplasm 3435 BY4741 03_1 16 C 7 YCL029C BIK1 PAC14|ARM5 Microtubule-associated protein; component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 mitotic cell cycle, cytoskeleton organization, regulation of organelle organization, organelle fusion, protein complex biogenesis, conjugation, nucleus organization cytoskeletal protein binding nucleus, cell cortex, cytoskeleton, microtubule organizing center, chromosome, site of polarized growth, cytoplasm 3436 BY4741 03_1 16 C 8 YCL030C HIS4 trifunctional histidinol dehydrogenase/phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase; phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis cellular amino acid metabolic process hydrolase activity, oxidoreductase activity cytoplasm 3437 BY4741 03_1 16 C 9 YCL032W STE50 - Adaptor protein for various signaling pathways; involved in mating response, invasive/filamentous growth, osmotolerance; acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction response to heat, response to chemical, protein phosphorylation, conjugation, response to osmotic stress, signaling enzyme regulator activity cytoplasm 3439 BY4741 03_1 16 C 10 YCL033C MXR2 MSRB Methionine-R-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan response to chemical, response to oxidative stress oxidoreductase activity mitochondrion, cytoplasm 3440 BY4741 03_1 16 C 11 YCL034W LSB5 - Protein involved in membrane-trafficking events at plasma membrane; interacts with actin regulators Sla1p and Las17p, ubiquitin, Arf3p to couple actin dynamics to membrane trafficking processes; similar structure to GGA family of proteins with N-terminal VHS domain and GAT domain; binds Las17p, which is homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly, actin polymerization; may mediate disassembly of Pan1 complex from endocytic coat cytoskeleton organization, endocytosis molecular_function cell cortex, cytoskeleton, cytoplasm 3441 BY4741 03_1 16 C 12 YCL036W GFD2 YCD6 Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication biological_process molecular_function cellular_component 3443 BY4741 03_1 16 D 1 YCL037C SRO9 - Cytoplasmic RNA-binding protein; shuttles between nucleus and cytoplasm and is exported from the nucleus in an mRNA export-dependent manner; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication cytoplasmic translation RNA binding, mRNA binding cytoplasm 3444 BY4741 03_1 16 D 2 YCL039W GID7 glucose-induced degradation complex subunit GID7|MOH2 Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process molecular_function nucleus, cytoplasm 3446 BY4741 03_1 16 D 3 YCL040W GLK1 glucokinase|HOR3 Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication carbohydrate metabolic process, cofactor metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, carbohydrate transport, nucleobase-containing small molecule metabolic process kinase activity, transferase activity membrane, cytoplasm, plasma membrane 3447 BY4741 03_1 16 D 4 YCL042W - - Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm biological_process molecular_function cytoplasm 3449 BY4741 03_1 16 D 5 YCL044C MGR1 - Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA proteolysis involved in cellular protein catabolic process other membrane, mitochondrial envelope, mitochondrion, cytoplasm 3451 BY4741 03_1 16 D 6 YCL045C EMC1 - Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 protein folding molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 3452 BY4741 03_1 16 D 7 YCL046W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C biological_process molecular_function cellular_component 3453 BY4741 03_1 16 D 8 YCL047C POF1 nicotinamide-nucleotide adenylyltransferase Nicotinamide mononucleotide-specific adenylyltransferase (NMNAT); catalyzes the conversion of nicotinamide mononucleotide (NMN) to nicotinamide adenine dinucleotide (NAD+); role in the nicotinamide riboside (NR) salvage pathway of NAD+ biosynthesis; involved in NR and NAD+ homeostasis; ATPase involved in protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion response to chemical, cofactor metabolic process, invasive growth in response to glucose limitation, proteolysis involved in cellular protein catabolic process, pseudohyphal growth, nucleobase-containing small molecule metabolic process transferase activity, nucleotidyltransferase activity, hydrolase activity, ATPase activity nucleus, cytoplasm 3454 BY4741 03_1 16 D 9 YCL048W SPS22 - Protein of unknown function; SPS22 has a paralog, SPS2, that arose from the whole genome duplication; redundant with Sps2p for the organization of the beta-glucan layer of the spore wall meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function membrane, plasma membrane 3455 BY4741 03_1 16 D 10 YCL049C - - Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene biological_process molecular_function membrane 3456 BY4741 03_1 16 D 11 YCL050C APA1 DTP1 AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication nucleobase-containing small molecule metabolic process transferase activity, nucleotidyltransferase activity, hydrolase activity nucleus, cytoplasm 3457 BY4741 03_1 16 D 12 YCL051W LRE1 - Protein involved in control of cell wall structure and stress response; direct inhibitor of the nuclear Dbf2 related (NDR) kinase Cbk1p-Mob2p; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; LRE1 has a paralog, HLR1, that arose from the whole genome duplication regulation of protein modification process, protein phosphorylation, cell wall organization or biogenesis enzyme regulator activity cellular bud, site of polarized growth 3458 BY4741 03_1 16 E 1 YCL055W KAR4 - Transcription factor required for response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone meiotic cell cycle, response to chemical, organelle fission, organelle fusion, conjugation, transcription from RNA polymerase II promoter, nucleus organization nucleic acid binding transcription factor activity nucleus, cytoplasm 3462 BY4741 03_1 16 E 2 YCL056C PEX34 - Protein that regulates peroxisome populations; peroxisomal integral membrane protein; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation peroxisome organization molecular_function peroxisome, membrane, cytoplasm 3463 BY4741 03_1 16 E 3 YCL057W PRD1 - Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress other peptidase activity, hydrolase activity endomembrane system, vacuole, mitochondrion, mitochondrial envelope, Golgi apparatus, cytoplasm 3464 BY4741 03_1 16 E 4 YCL060C endomembrane system, vacuole, mitochondrion, mitochondrial envelope, Golgi apparatus, cytoplasm endomembrane system, vacuole, mitochondrion, mitochondrial envelope, Golgi apparatus, cytoplasm endomembrane system, vacuole, mitochondrion, mitochondrial envelope, Golgi apparatus, cytoplasm - - - 3467 BY4741 03_1 16 E 5 YCL061C MRC1 chromatin-modulating protein MRC1|YCL060C S-phase checkpoint protein required for DNA replication; couples DNA helicase and polymerase; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; defines a novel S-phase checkpoint with Hog1p that coordinates DNA replication and transcription upon osmostress; protects uncapped telomeres; Dia2p-dependent degradation mediates checkpoint recovery; mammalian claspin homolog mitotic cell cycle, DNA repair, telomere organization, organelle fission, chromatin organization, DNA replication, cellular response to DNA damage stimulus, chromosome segregation, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, chromosome 3468 BY4741 03_1 16 E 6 YCL062W nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - 3469 BY4741 03_1 16 E 7 YCL063W VAC17 YCL062W Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p vacuole organization, organelle inheritance other membrane, vacuole, cytoplasm 3470 BY4741 03_1 16 E 8 YCL064C CHA1 L-serine/L-threonine ammonia-lyase CHA1 Catabolic L-serine (L-threonine) deaminase; catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine cellular amino acid metabolic process lyase activity mitochondrion, cytoplasm 3471 BY4741 03_1 16 E 9 YCL069W VBA3 - Permease of basic amino acids in the vacuolar membrane; VBA3 has a paralog, VBA5, that arose from a segmental duplication transmembrane transport, ion transport, amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm 3476 BY4741 03_1 16 E 10 YCR001W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene biological_process molecular_function cellular_component 3481 BY4741 03_1 16 E 11 YCR002C CDC10 septin CDC10 Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress mitotic cell cycle, organelle fission, cytoskeleton organization, sporulation, cytokinesis, organelle assembly, protein complex biogenesis GTPase activity, hydrolase activity, ion binding, lipid binding, structural molecule activity cell cortex, cytoskeleton, cellular bud, membrane, site of polarized growth, cytoplasm 3482 BY4741 03_1 16 E 12 YCR003W MRPL32 mitochondrial 54S ribosomal protein YmL32|YmL32 Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress mitochondrial translation, mitochondrion organization structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, membrane, mitochondrial envelope, cytoplasm 3483 BY4741 03_1 16 F 1 YCR004C YCP4 - Protein of unknown function; has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process other membrane, mitochondrion, plasma membrane, cytoplasm 3484 BY4741 03_1 16 F 2 YCR005C CIT2 citrate (Si)-synthase CIT2 Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle; catalyzes condensation of acetyl coenzyme A and oxaloacetate to form citrate; expression is controlled by Rtg1p and Rtg2p transcription factors; SCF-Ucc1 regulates level of Cit2p to maintain citrate homeostasis; oxaloacetate-dependent positive feedback loop inhibits Cit2p ubiquitination; CIT2 has a paralog, CIT1, that arose from the whole genome duplication carbohydrate metabolic process, monocarboxylic acid metabolic process, cellular amino acid metabolic process transferase activity peroxisome, mitochondrion, cytoplasm 3485 BY4741 03_1 16 F 3 YCR006C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 3486 BY4741 03_1 16 F 4 YCR007C - DUP240 family protein Putative integral membrane protein; member of DUP240 gene family; YCR007C is not an essential gene biological_process molecular_function cellular_component 3487 BY4741 03_1 16 F 5 YCR008W SAT4 serine/threonine protein kinase SAT4|HAL4 Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; overexpression affects the Fe-S and lipoamide containing proteins in the mitochondrion; required for lipoylation of Lat1p, Kgd2p and Gcv3p; partially redundant with Hal5p; has similarity to Npr1p; localizes to the cytoplasm and mitochondrion mitotic cell cycle, protein phosphorylation, protein dephosphorylation, cellular ion homeostasis kinase activity, transferase activity mitochondrion, cytoplasm 3488 BY4741 03_1 16 F 6 YCR009C RVS161 amphiphysin-like protein RVS161|SPE161|FUS7|END6 Amphiphysin-like lipid raft protein; N-BAR domain protein that interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress cytoskeleton organization, conjugation, response to starvation, response to osmotic stress, endocytosis lipid binding, cytoskeletal protein binding cell cortex, cytoskeleton, membrane, site of polarized growth, cytoplasm 3489 BY4741 03_1 16 F 7 YCR010C ADY2 ATO1 Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm 3490 BY4741 03_1 16 F 8 YCR011C ADP1 putative ATP-dependent permease ADP1 Putative ATP-dependent permease of the ABC transporter family transmembrane transport hydrolase activity, transmembrane transporter activity, ATPase activity endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm 3491 BY4741 03_1 16 F 9 YCR014C POL4 DNA-directed DNA polymerase IV|POLX DNA polymerase IV; undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta DNA repair, cellular response to DNA damage stimulus transferase activity, nucleotidyltransferase activity nucleus 3494 BY4741 03_1 16 F 10 YCR015C CTO1 - Protein required for cold tolerance; involved in phosphate uptake; YCR015C is not an essential gene other molecular_function cellular_component 3495 BY4741 03_1 16 F 11 YCR016W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis biological_process molecular_function nucleus, nucleolus 3496 BY4741 03_1 16 F 12 YCR017C CWH43 - Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion lipid metabolic process, cell wall organization or biogenesis, protein lipidation molecular_function cellular bud, membrane, site of polarized growth, plasma membrane 3497 BY4741 03_1 16 G 1 YCR019W MAK32 - Protein necessary for stability of L-A dsRNA-containing particles other molecular_function cellular_component 3499 BY4741 03_1 16 G 2 YCR020C PET18 HIT2 Protein of unknown function; has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin vitamin metabolic process molecular_function cytoplasm 3500 BY4741 03_1 16 G 3 YCR020C-A MAK31 NAA38|SMX1|LSM9 Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family protein acylation transferase activity cytoplasm 3501 BY4741 03_1 16 G 4 YCR021C HSP30 YRO1 Negative regulator of the H(+)-ATPase Pma1p; stress-responsive protein; hydrophobic plasma membrane localized; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase response to heat, response to chemical, cellular response to DNA damage stimulus, response to oxidative stress, response to osmotic stress molecular_function membrane, plasma membrane 3502 BY4741 03_1 16 G 5 YCR022C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene biological_process molecular_function cellular_component 3503 BY4741 03_1 16 G 6 YCR023C - - Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene biological_process molecular_function membrane, vacuole, cytoplasm 3504 BY4741 03_1 16 G 7 YLR420W URA4 dihydroorotase Dihydroorotase; catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate nucleobase-containing small molecule metabolic process hydrolase activity nucleus, cytoplasm 4081 BY4741 12_3 16 G 8 YLR451W LEU3 leucine-responsive transcriptional regulator LEU3 Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus 4082 BY4741 12_3 16 G 9 YLR126C - putative amidotransferase Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress cellular ion homeostasis molecular_function nucleus, cytoplasm 4083 BY4741 12_3 16 G 10 YLR128W DCN1 NEDD8 ligase DCN1 Scaffold-type E3 ligase; required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation regulation of protein modification process, protein modification by small protein conjugation or removal enzyme binding, protein binding, bridging, ubiquitin-like protein binding cellular_component 4085 BY4741 12_3 16 G 11 YLR130C ZRT2 low-affinity Zn(2+) transporter ZRT2 Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor transmembrane transport, ion transport transmembrane transporter activity membrane, plasma membrane 4087 BY4741 12_3 16 G 12 YLR131C ACE2 DNA-binding transcription factor ACE2 Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication mitotic cell cycle, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 4088 BY4741 12_3 16 H 1 YLR133W CKI1 bifunctional choline kinase/ethanolamine kinase CKI1 Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication lipid metabolic process kinase activity, transferase activity cytoplasm 4090 BY4741 12_3 16 H 3 YLR134W PDC5 indolepyruvate decarboxylase 5 Minor isoform of pyruvate decarboxylase; key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism carbohydrate metabolic process, cofactor metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, cellular amino acid metabolic process, nucleobase-containing small molecule metabolic process lyase activity nucleus, cytoplasm 4091 BY4741 12_3 16 H 4 YLR135W SLX4 - Endonuclease involved in processing DNA; acts during recombination, repair; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); cleaves branched structures in complex with Slx1p; involved in interstrand cross-link repair, Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress; FANCP-related factor DNA repair, DNA replication, DNA recombination, cellular response to DNA damage stimulus nuclease activity, hydrolase activity nucleus, chromosome 4092 BY4741 12_3 16 H 5 YLR136C TIS11 CTH2 mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; TIS11 has a paralog, CTH1, that arose from the whole genome duplication RNA catabolic process, cellular ion homeostasis RNA binding, mRNA binding nucleus, cytoplasm 4093 BY4741 12_3 16 H 6 YLR137W RKM5 - Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species protein alkylation transferase activity, methyltransferase activity cellular_component 4094 BY4741 12_3 16 H 7 YLR138W NHA1 - Na+/H+ antiporter; involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH cellular ion homeostasis, transmembrane transport, ion transport, response to osmotic stress transmembrane transporter activity membrane, plasma membrane 4095 BY4741 12_3 16 H 8 YLR139C SLS1 - Mitochondrial membrane protein; coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery mitochondrial translation, mitochondrion organization, generation of precursor metabolites and energy, cellular respiration molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm 4096 BY4741 12_3 16 H 9 YLR142W PUT1 proline dehydrogenase Proline oxidase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 4099 BY4741 12_3 16 H 10 YLR143W DPH6 diphthine--ammonia ligase Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene peptidyl-amino acid modification ligase activity cytoplasm 4100 BY4741 12_3 16 H 11 YLR144C ACF2 PCA1|ENG2 Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress cytoskeleton organization hydrolase activity, hydrolase activity, acting on glycosyl bonds other 4101 BY4741 12_3 16 H 12 YMR074C SDD2 - Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress other molecular_function nucleus, cytoplasm 7118 BY4741 00_15 17 A 1 YLR149C - - Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress biological_process molecular_function cellular_component 4106 BY4741 12_3 17 A 2 YLR150W STM1 MPT4 Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery translational initiation, telomere organization, regulation of translation, translational elongation, signaling DNA binding cytoplasm 4107 BY4741 12_3 17 A 3 YLR151C PCD1 8-oxo-dGTP diphosphatase 8-oxo-dGTP diphosphatase; prevents spontaneous mutagenesis via sanitization of oxidized purine nucleoside triphosphates; can also act as peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member; similar E. coli MutT and human, rat and mouse MTH1 DNA repair, cofactor metabolic process, nucleobase-containing small molecule metabolic process, cellular response to DNA damage stimulus hydrolase activity peroxisome, cytoplasm 4108 BY4741 12_3 17 A 4 YLR152C - - Putative protein of unknown function; YLR152C is not an essential gene biological_process molecular_function membrane 4109 BY4741 12_3 17 A 5 YLR154C RNH203 Rnh2C Ribonuclease H2 subunit; required for RNase H2 activity; role in ribonucleotide excision repair; related to human AGS3 that causes Aicardi-Goutieres syndrome RNA catabolic process, DNA replication nuclease activity, hydrolase activity nucleus, cytoplasm 4111 BY4741 12_3 17 A 6 YLR164W SHH4 protein SHH4 Putative alternate subunit of succinate dehydrogenase (SDH); mitochondrial inner membrane protein; genetic interaction with SDH4 suggests that Shh4p can function as a functional SDH subunit; a fraction copurifies with SDH subunit Sdh3p; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner; Shh4p has greater similarity to human SDHD (subunit D of SDH, implicated in paraganglioma) than does its paralog Sdh4p generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 4113 BY4741 12_3 17 A 7 YLR165C PUS5 pseudouridine synthase PUS5 Pseudouridine synthase; catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability rRNA processing, RNA modification lyase activity mitochondrion, cytoplasm 4114 BY4741 12_3 17 A 8 YLR168C UPS2 GEP1|AIM30|MSF1|MSF1' Mitochondrial intermembrane space protein; involved in phospholipid metabolism; has role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI; UPS2 has a paralog, UPS3, that arose from the whole genome duplication lipid metabolic process, mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4117 BY4741 12_3 17 A 9 YLR169W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4118 BY4741 12_3 17 A 10 YLR170C APS1 YAP19 Small subunit of the clathrin-associated adaptor complex AP-1; AP-1 is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Golgi vesicle transport other membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 4119 BY4741 12_3 17 A 11 YLR171W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane 4120 BY4741 12_3 17 A 12 YLR172C DPH5 diphthine synthase Methyltransferase required for synthesis of diphthamide; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm peptidyl-amino acid modification transferase activity, methyltransferase activity cytoplasm 4121 BY4741 12_3 17 B 1 YLR173W - - Putative protein of unknown function biological_process molecular_function membrane, vacuole, cytoplasm 4122 BY4741 12_3 17 B 2 YLR174W IDP2 isocitrate dehydrogenase (NADP(+)) IDP2 Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication other oxidoreductase activity cytoplasm 4123 BY4741 12_3 17 B 3 YLR176C RFX1 CRT1 Major transcriptional repressor of DNA-damage-regulated genes; recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins transcription from RNA polymerase II promoter DNA binding nucleus, cytoplasm 4125 BY4741 12_3 17 B 4 YLR177W - - Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication biological_process molecular_function cytoplasm 4126 BY4741 12_3 17 B 6 YLR178C TFS1 DKA1 Inhibitor of carboxypeptidase Y (Prc1p), and Ras GAP (Ira2p); phosphatidylethanolamine-binding protein (PEBP) family member and ortholog of hPEBP1/RKIP, a natural metastasis suppressor; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress signaling ion binding, lipid binding, enzyme regulator activity membrane, vacuole, cytoplasm 4127 BY4741 12_3 17 B 7 YLR179C - - Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus, cytoplasm 4128 BY4741 12_3 17 B 8 YLR180W SAM1 methionine adenosyltransferase SAM1|ETH10 S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication cofactor metabolic process, nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process transferase activity cytoplasm 4129 BY4741 12_3 17 B 9 YLR181C VTA1 - Multivesicular body (MVB) protein; involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain endosomal transport, protein complex biogenesis enzyme regulator activity membrane, endomembrane system, cytoplasm 4130 BY4741 12_3 17 B 10 YLR182W SWI6 transcriptional regulator SWI6|SDS11|PSL8 Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators response to heat, mitotic cell cycle, meiotic cell cycle, organelle fission, regulation of organelle organization, DNA recombination, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, regulation of cell cycle transcription factor activity, protein binding nucleus, cytoplasm 4131 BY4741 12_3 17 B 11 YLR183C TOS4 - Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; involved in DNA replication checkpoint response; interacts with Rpd3 and Set3 histone deacetylase (HDAC) complexes; APCC(Cdh1) substrate; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication cellular response to DNA damage stimulus chromatin binding nucleus, chromosome, mitochondrion, cytoplasm 4132 BY4741 12_3 17 B 12 YLR184W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4133 BY4741 12_3 17 C 1 YLR185W RPL37A ribosomal 60S subunit protein L37A|L37e|YL35|L43|L37A Ribosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 4134 BY4741 12_3 17 C 2 YLR187W SKG3 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication biological_process molecular_function cellular bud, site of polarized growth, cytoplasm 4136 BY4741 12_3 17 C 3 YLR188W MDL1 ATP-binding cassette permease MDL1 Mitochondrial inner membrane half-type ABC transporter; mediates export of peptides generated upon proteolysis of mitochondrial proteins; plays a role in the regulation of cellular resistance to oxidative stress transmembrane transport ATPase activity, hydrolase activity, transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 4137 BY4741 12_3 17 C 4 YLR189C ATG26 UGT51 UDP-glucose:sterol glucosyltransferase; conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy meiotic cell cycle, lipid metabolic process, sporulation transferase activity, transferring glycosyl groups, transferase activity cytoplasm 4138 BY4741 12_3 17 C 5 YLR190W MMR1 - Phosphorylated protein of the mitochondrial outer membrane; localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p mitochondrion organization, organelle inheritance molecular_function mitochondrion, cellular bud, membrane, site of polarized growth, mitochondrial envelope, cytoplasm 4139 BY4741 12_3 17 C 6 YLR191W PEX13 peroxin PEX13|PAS20 Peroxisomal importomer complex component; integral peroxisomal membrane protein required for docking and translocation of peroxisomal matrix proteins; interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p; forms a complex with Pex14p and Pex17p; human homolog PEX13 complements yeast null mutant protein targeting, transmembrane transport, peroxisome organization protein binding, bridging peroxisome, membrane, cytoplasm 4140 BY4741 12_3 17 C 7 YLR193C UPS1 - Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI ion transport, lipid metabolic process, lipid transport other mitochondrion, membrane, mitochondrial envelope, cytoplasm 4142 BY4741 12_3 17 C 8 YLR194C - - Structural constituent of the cell wall; attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress cell wall organization or biogenesis structural molecule activity cell wall, membrane, plasma membrane 4143 BY4741 12_3 17 C 9 YLR199C PBA1 POC1 Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly protein complex biogenesis molecular_function cytoplasm 4148 BY4741 12_3 17 C 10 YLR200W YKE2 tubulin-binding prefolding complex subunit YKE2|PFD6|GIM1 Subunit of the heterohexameric Gim/prefoldin protein complex; involved in the folding of alpha-tubulin, beta-tubulin, and actin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation protein folding, transcription from RNA polymerase II promoter, protein complex biogenesis, DNA-templated transcription, elongation cytoskeletal protein binding cytoplasm 4149 BY4741 12_3 17 C 11 YLR201C COQ9 ubiquinone biosynthesis protein COQ9|FMP53 Protein required for ubiquinone biosynthesis and respiratory growth; localizes to matrix face of mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; ubiquinone is also known as coenzyme Q; human homolog COQ9 can complement yeast coq9 null mutant cofactor metabolic process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4150 BY4741 12_3 17 C 12 YLR202C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron biological_process molecular_function cellular_component 4151 BY4741 12_3 17 D 1 YLR203C MSS51 - Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; localizes to vacuole membrane in response to H2O2; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other vacuole, mitochondrion, membrane, mitochondrial envelope, cytoplasm 4152 BY4741 12_3 17 D 2 YLR204W QRI5 COX24 Mitochondrial inner membrane protein; required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA mRNA processing molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4153 BY4741 12_3 17 D 3 YLR205C HMX1 - ER localized heme oxygenase; involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants cofactor metabolic process, response to oxidative stress, cellular ion homeostasis oxidoreductase activity nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 4154 BY4741 12_3 17 D 4 YLR206W ENT2 - Epsin-like protein required for endocytosis and actin patch assembly; functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus; ENT2 has a paralog, ENT1, that arose from the whole genome duplication cytoskeleton organization, endocytosis other site of polarized growth 4155 BY4741 12_3 17 D 5 YLR207W HRD3 ubiquitin ligase complex subunit HRD3 ER membrane protein that plays a central role in ERAD; forms HRD complex with Hrd1p and ER-associated protein degradation (ERAD) determinants that engages in lumen to cytosol communication and coordination of ERAD events response to chemical, proteolysis involved in cellular protein catabolic process transferase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 4156 BY4741 12_3 17 D 6 YLR209C PNP1 purine-nucleoside phosphorylase Purine nucleoside phosphorylase; specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway cofactor metabolic process, nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups, transferase activity, hydrolase activity, hydrolase activity, acting on glycosyl bonds other 4158 BY4741 12_3 17 D 7 YLR210W CLB4 B-type cyclin CLB4 B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; CLB4 has a paralog, CLB3, that arose from the whole genome duplication mitotic cell cycle, protein phosphorylation, organelle fission, cytoskeleton organization, regulation of organelle organization, organelle assembly, protein complex biogenesis, regulation of protein modification process, regulation of cell cycle enzyme regulator activity nucleus, cytoskeleton, microtubule organizing center, cytoplasm 4159 BY4741 12_3 17 D 8 YLR211C ATG38 - Homodimeric subunit of autophagy-specific PtdIns-3-kinase complex I; required for the integrity of the active PtdIns-3-kinase complex I by maintaining an association between Vps15p-Vps34p and Atg14p-Vps30p subcomplexes; localizes to the pre-autophagosomal structure (PAS) in an Atg14p-dependent manner; ATG38 is non-essential but is required for macroautophagy other structural molecule activity membrane, cytoplasm 4160 BY4741 12_3 17 D 9 YLR213C CRR1 - Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation meiotic cell cycle, sporulation, cell wall organization or biogenesis hydrolase activity cell wall 4162 BY4741 12_3 17 D 10 YLR214W FRE1 ferric/cupric-chelate reductase Ferric reductase and cupric reductase; reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels ion transport oxidoreductase activity membrane, plasma membrane 4163 BY4741 12_3 17 D 11 YLR216C CPR6 peptidylprolyl isomerase CPR6|CYP40 Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; plays a role in determining prion variants; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress protein folding unfolded protein binding, isomerase activity cytoplasm 4165 BY4741 12_3 17 D 12 YLR217W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 biological_process molecular_function cellular_component 4166 BY4741 12_3 17 E 1 YLR218C COA4 CMC3 Twin Cx(9)C protein involved in cytochrome c oxidase organization; organization includes assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors mitochondrion organization, protein complex biogenesis molecular_function nucleus, mitochondrial envelope, mitochondrion, cytoplasm 4167 BY4741 12_3 17 E 2 YLR219W MSC3 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress meiotic cell cycle, organelle fission, DNA recombination molecular_function cellular_component 4168 BY4741 12_3 17 E 3 YLR220W CCC1 - Vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress ion transport, cellular ion homeostasis transmembrane transporter activity endomembrane system, vacuole, membrane, Golgi apparatus, cytoplasm 4169 BY4741 12_3 17 E 4 YLR221C RSA3 - Protein with a likely role in ribosomal maturation; required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus ribosome assembly, organelle assembly, ribosomal large subunit biogenesis molecular_function nucleus, nucleolus 4170 BY4741 12_3 17 E 5 YLR224W UCC1 - F-box protein and component of SCF ubiquitin ligase complexes; involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; SCF-Ucc1 regulates level of Cit2 citrate synthase protein to maintain citrate homeostasis, acts as metabolic switch for glyoxylate cycle; UCC1 transcription is downregulated in cells grown on C2-compounds response to chemical, proteolysis involved in cellular protein catabolic process protein binding, bridging, transferase activity other 4173 BY4741 12_3 17 E 6 YLR225C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YLR225C has a paralog, YDR222W, that arose from the whole genome duplication biological_process molecular_function cytoplasm 4174 BY4741 12_3 17 E 7 YLR227C ADY4 - Structural component of the meiotic outer plaque; outer plaque is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane sporulation structural molecule activity cytoskeleton, microtubule organizing center 4176 BY4741 12_3 17 E 8 YKL001C MET14 adenylyl-sulfate kinase Adenylylsulfate kinase; required for sulfate assimilation and involved in methionine metabolism; human homolog PAPSS2 complements yeast null mutant cellular amino acid metabolic process kinase activity, transferase activity cytoplasm 4849 BY4741 11_1 17 E 9 YKL002W DID4 ESCRT-III subunit protein DID4|VPS14|CHM2|VPS2|VPT14|VPL2|REN1|GRD7 Class E Vps protein of the ESCRT-III complex; required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis endosomal transport, vesicle organization, proteolysis involved in cellular protein catabolic process molecular_function membrane, endomembrane system, cytoplasm 4850 BY4741 11_1 17 E 10 YKL003C MRP17 mitochondrial 37S ribosomal protein YmS16 Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4851 BY4741 11_1 17 E 11 YKL006W RPL14A ribosomal 60S subunit protein L14A|L14e|L14A Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 4855 BY4741 11_1 17 E 12 YKL007W CAP1 - Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress cytoskeleton organization, regulation of organelle organization, protein complex biogenesis cytoskeletal protein binding cell cortex, cytoskeleton, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane 4856 BY4741 11_1 17 F 1 YKL008C LAC1 sphingosine N-acyltransferase LAC1|DGT1 Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p; LAC1 has a paralog, LAG1, that arose from the whole genome duplication lipid metabolic process transferase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 4857 BY4741 11_1 17 F 2 YKL009W MRT4 - Protein involved in mRNA turnover and ribosome assembly; required at post-transcriptional step for efficient retrotransposition; localizes to the nucleolus rRNA processing, organelle assembly, ribosomal large subunit biogenesis, RNA catabolic process, ribosome assembly RNA binding, rRNA binding nucleus, nucleolus 4858 BY4741 11_1 17 F 3 YKL010C UFD4 putative ubiquitin-protein ligase UFD4 Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress DNA replication, protein modification by small protein conjugation or removal, regulation of DNA metabolic process, protein complex biogenesis transferase activity nucleus, mitochondrion, cytoplasm 4859 BY4741 11_1 17 F 4 YKL011C CCE1 cruciform cutting endonuclease|MGT1 Mitochondrial cruciform cutting endonuclease; cleaves Holliday junctions formed during recombination of mitochondrial DNA; CCE1 has a paralog, MRS1, that arose from the whole genome duplication mitochondrion organization nuclease activity, hydrolase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 4860 BY4741 11_1 17 F 5 YKL015W PUT3 - Transcriptional activator; binds specific gene recruitment sequences and is required for DNA zip code-mediated targeting of genes to nuclear periphery; regulates proline utilization genes, constitutively binds PUT1 and PUT2 promoters as a dimer, undergoes conformational change to form active state; binds other promoters only under activating conditions; differentially phosphorylated in presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus 4864 BY4741 11_1 17 F 6 YKL016C ATP7 F1F0 ATP synthase subunit d Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis transmembrane transport, ion transport, nucleobase-containing small molecule metabolic process hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 4865 BY4741 11_1 17 F 7 YKL017C HCS1 ATP-dependent 5'-3' DNA helicase HCS1|DIP1 Hexameric DNA polymerase alpha-associated DNA helicase A; involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities DNA replication helicase activity, ATPase activity, hydrolase activity nucleus, chromosome 4866 BY4741 11_1 17 F 8 YKL020C SPT23 - ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; SPT23 has a paralog, MGA2, that arose from the whole genome duplication chromatin organization, monocarboxylic acid metabolic process, lipid metabolic process, transcription from RNA polymerase II promoter DNA binding nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 4869 BY4741 11_1 17 F 9 YKL023W - - Putative protein of unknown function; predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm 4872 BY4741 11_1 17 F 10 YKL025C PAN3 ECM35 Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex; poly (A) mRNA binding subunit which recruits mRNA to the complex; the Pan2p-Pan3p complex controls poly(A) tail length and regulates the stoichiometry and activity of postreplication repair complexes RNA catabolic process, DNA repair, cellular response to DNA damage stimulus RNA binding, nuclease activity, hydrolase activity cytoplasm 4874 BY4741 11_1 17 F 11 YKL026C GPX1 glutathione peroxidase GPX1 Phospholipid hydroperoxide glutathione peroxidase; induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; GPX1 has a paralog, HYR1, that arose from the whole genome duplication response to chemical, peroxisome organization, response to oxidative stress oxidoreductase activity peroxisome, mitochondrion, membrane, mitochondrial envelope, cytoplasm 4875 BY4741 11_1 17 F 12 YKL027W TCD2 - tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD2 has a paralog, TCD1, that arose from the whole genome duplication tRNA processing, RNA modification lyase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 4876 BY4741 11_1 17 G 1 YKL029C MAE1 malate dehydrogenase (oxaloacetate-decarboxylating) Mitochondrial malic enzyme; catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids monocarboxylic acid metabolic process, cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 4878 BY4741 11_1 17 G 2 YKL031W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species biological_process molecular_function cellular_component 4880 BY4741 11_1 17 G 3 YKL032C IXR1 DNA-binding transcription repressor IXR1|ORD1 Transcriptional repressor that regulates hypoxic genes during normoxia; involved in the aerobic repression of genes such as COX5b, TIR1, and HEM13; binds DNA intrastrand cross-links formed by cisplatin; HMG (high mobility group box) domain containing protein which binds and bends cisplatin-modified DNA, blocking excision repair; IXR1 has a paralog, ABF2, that arose from the whole genome duplication DNA repair, response to chemical, cellular response to DNA damage stimulus, transcription from RNA polymerase II promoter DNA binding nucleus, chromosome 4881 BY4741 11_1 17 G 4 YKL034W TUL1 ubiquitin-protein ligase TUL1 Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process transferase activity membrane, endomembrane system, cytoplasm, Golgi apparatus 4883 BY4741 11_1 17 G 5 YKL037W AIM26 - Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT mitochondrion organization molecular_function membrane, mitochondrion, cytoplasm 4886 BY4741 11_1 17 G 6 YKL038W RGT1 - Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; recruits Tup1p/Cyc8p to target gene promoters; RGT1 has a paralog, EDS1, that arose from the whole genome duplication carbohydrate metabolic process, carbohydrate transport, regulation of transport, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding nucleus 4887 BY4741 11_1 17 G 7 YKL039W PTM1 - Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication biological_process molecular_function membrane 4888 BY4741 11_1 17 G 8 YKL040C NFU1 NUB1 Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria cellular ion homeostasis molecular_function mitochondrion, cytoplasm 4889 BY4741 11_1 17 G 9 YKL041W VPS24 ESCRT-III subunit protein VPS24|VPL26|DID3 One of four subunits of the ESCRT-III complex; forms an endosomal sorting complex required for transport III (ESCRT-III) subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway endosomal transport, vesicle organization, proteolysis involved in cellular protein catabolic process molecular_function membrane, endomembrane system, cytoplasm 4890 BY4741 11_1 17 G 10 YKL043W PHD1 - Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication pseudohyphal growth, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 4892 BY4741 11_1 17 G 11 YKL044W - - Protein of unknown function; mRNA identified as translated by ribosome profiling data biological_process molecular_function cellular_component 4893 BY4741 11_1 17 G 12 YKL046C DCW1 putative mannan endo-1,6-alpha-mannosidase Putative mannosidase; GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p cell budding, cell wall organization or biogenesis molecular_function cellular bud, membrane, endomembrane system, endoplasmic reticulum, plasma membrane, cytoplasm 4895 BY4741 11_1 17 H 1 YKL047W ANR2 - Putative protein of unknown function; may have a role in lipid metabolism, based on localization to lipid droplets; predicted to be palmitoylated biological_process molecular_function cytoplasm 4896 BY4741 11_1 17 H 3 YKL048C ELM1 serine/threonine protein kinase ELM1|LDB9 Serine/threonine protein kinase that regulates cellular morphogenesis; septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring mitotic cell cycle, carbohydrate metabolic process, response to chemical, protein phosphorylation, cell morphogenesis, cell budding, cytoskeleton organization, organelle assembly, cytokinesis, protein complex biogenesis, regulation of protein modification process, pseudohyphal growth kinase activity, transferase activity cellular bud, site of polarized growth 4897 BY4741 11_1 17 H 4 YKL050C - - Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p; YKL050C has a paralog, EIS1, that arose from the whole genome duplication biological_process molecular_function cellular_component 4899 BY4741 11_1 17 H 5 YKL051W SFK1 - Plasma membrane protein that may act to generate normal levels of PI4P; may act together with or upstream of Stt4p; at least partially mediates proper localization of Stt4p to the plasma membrane vacuole organization, cytoskeleton organization, signaling molecular_function membrane, plasma membrane 4900 BY4741 11_1 17 H 6 YKL053W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 biological_process molecular_function membrane 4902 BY4741 11_1 17 H 7 YKL054C DEF1 DNA damage-responsive RNA polymerase-degradation factor DEF1|VID31 RNAPII degradation factor; forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis DNA repair, telomere organization, protein modification by small protein conjugation or removal, cellular response to DNA damage stimulus, proteolysis involved in cellular protein catabolic process molecular_function nucleus 4903 BY4741 11_1 17 H 8 YKL055C OAR1 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase; may comprise a type II mitochondrial fatty acid synthase along with Mct1p; human homolog CBR4 complements yeast null mutant generation of precursor metabolites and energy, monocarboxylic acid metabolic process, cellular respiration, lipid metabolic process transferase activity, oxidoreductase activity mitochondrion, cytoplasm 4904 BY4741 11_1 17 H 9 YKL056C TMA19 MMI1|RBF18 Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress cytoplasmic translation, response to chemical, response to oxidative stress molecular_function ribosome, mitochondrion, cytoplasm 4905 BY4741 11_1 17 H 10 YKL057C NUP120 RAT2 Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 response to heat, DNA repair, protein targeting, chromatin organization, cellular response to DNA damage stimulus, ribosomal subunit export from nucleus, nucleobase-containing compound transport, transcription from RNA polymerase II promoter, nuclear transport, nucleus organization structural molecule activity nucleus, endomembrane system, membrane 4906 BY4741 11_1 17 H 11 YKL061W BLI1 - Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; green fluorescent protein (GFP)-fusion protein localizes to the endosome other molecular_function endomembrane system, cytoplasm 4910 BY4741 11_1 17 H 12 YKL062W MSN4 stress-responsive transcriptional activator MSN4 Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes, inducing gene expression; involved in diauxic shift response to heat, response to chemical, chromatin organization, response to oxidative stress, response to starvation, transcription from RNA polymerase II promoter, response to osmotic stress nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 4911 BY4741 11_1 18 A 1 YKL063C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi biological_process molecular_function endomembrane system, cytoplasm, Golgi apparatus 4912 BY4741 11_1 18 A 2 YKL064W MNR2 putative Mg(2+) transporter MNR2 Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs) cellular ion homeostasis transmembrane transporter activity membrane, vacuole, cytoplasm 4913 BY4741 11_1 18 A 3 YKL065C YET1 - Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein; YET1 has a paralog, YET2, that arose from the whole genome duplication biological_process molecular_function membrane, endomembrane system, ribosome, endoplasmic reticulum, cytoplasm 4914 BY4741 11_1 18 A 4 YKL066W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1 biological_process molecular_function cellular_component 4915 BY4741 11_1 18 A 5 YKL067W YNK1 nucleoside diphosphate kinase|NDK1 Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress nucleobase-containing small molecule metabolic process, cellular response to DNA damage stimulus kinase activity, transferase activity mitochondrial envelope, mitochondrion, cytoplasm 4916 BY4741 11_1 18 A 6 YKL068W NUP100 FG-nucleoporin NUP100|[NUP100+]|NSP100 FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; NUP100 has a paralog, NUP116, that arose from the whole genome duplication response to heat, protein targeting, nucleobase-containing compound transport, nuclear transport, nucleus organization structural molecule activity nucleus, endomembrane system, membrane 4917 BY4741 11_1 18 A 7 YKL069W - L-methionine (R)-S-oxide reductase|fRMsr|YKG9 Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress response to chemical, response to oxidative stress oxidoreductase activity nucleus, cytoplasm 4918 BY4741 11_1 18 A 8 YKL070W - - Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 4919 BY4741 11_1 18 A 9 YKL071W - - Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function membrane, cytoplasm 4920 BY4741 11_1 18 A 10 YKL072W STB6 - Protein that binds Sin3p in a two-hybrid assay; STB6 has a paralog, STB2, that arose from the whole genome duplication biological_process molecular_function cellular_component 4921 BY4741 11_1 18 A 11 YKL073W LHS1 Hsp70 family chaperone LHS1|SSI1|CER1 Molecular chaperone of the endoplasmic reticulum lumen; involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway response to chemical, protein targeting, transmembrane transport enzyme regulator activity, ion binding, unfolded protein binding endomembrane system, endoplasmic reticulum, cytoplasm 4922 BY4741 11_1 18 A 12 YKL074C MUD2 - Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 RNA splicing, mRNA processing RNA binding, mRNA binding nucleus 4923 BY4741 11_1 18 B 1 YKL075C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin response to chemical molecular_function cytoplasm 4924 BY4741 11_1 18 B 2 YKL076C PSY1 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C biological_process molecular_function cellular_component 4925 BY4741 11_1 18 B 3 YKL077W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function vacuole, cytoplasm 4926 BY4741 11_1 18 B 4 YKL079W SMY1 - Kinesin-like myosin passenger-protein; interacts with Myo2p; controls actin cable structure and dynamics; proposed to be involved in exocytosis exocytosis hydrolase activity cellular bud, site of polarized growth 4928 BY4741 11_1 18 B 5 YKL080W VMA5 H(+)-transporting V1 sector ATPase subunit C|VAT3|CSL5 Subunit C of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits cellular ion homeostasis ATPase activity, hydrolase activity, transmembrane transporter activity membrane, vacuole, cytoplasm 4929 BY4741 11_1 18 B 7 YKL081W TEF4 EFC1 Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex translational elongation guanyl-nucleotide exchange factor activity, RNA binding, translation factor activity, RNA binding ribosome, mitochondrion, cytoplasm 4930 BY4741 11_1 18 B 8 YKL084W HOT13 - Zinc-binding mitochondrial intermembrane space (IMS) protein; involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc protein targeting, mitochondrion organization, transmembrane transport ion binding mitochondrial envelope, mitochondrion, cytoplasm 4933 BY4741 11_1 18 B 9 YKL085W MDH1 malate dehydrogenase MDH1 Mitochondrial malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated generation of precursor metabolites and energy, cellular respiration RNA binding, mRNA binding, oxidoreductase activity mitochondrion, cytoplasm 4934 BY4741 11_1 18 B 10 YKL086W SRX1 sulfiredoxin Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress response to chemical, response to oxidative stress oxidoreductase activity nucleus, cytoplasm 4935 BY4741 11_1 18 B 11 YKL087C CYT2 cytochrome c1 heme lyase CYT2|CC1HL Cytochrome c1 heme lyase; involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1; human homolog HCCS can complement yeast cyt2 null mutant protein complex biogenesis lyase activity mitochondrial envelope, mitochondrion, cytoplasm 4936 BY4741 11_1 18 B 12 YKL090W CUE2 - Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination biological_process ubiquitin-like protein binding cellular_component 4939 BY4741 11_1 18 C 1 YKL091C - phosphatidylinositol/phosphatidylcholine-binding protein|SFH1 Putative phosphatidylinositol/phosphatidylcholine transfer protein; possibly involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study; YKL091C has a paralog, SEC14, that arose from the whole genome duplication biological_process ion binding, lipid binding nucleus 4940 BY4741 11_1 18 C 2 YKL092C BUD2 ERC25|CLA2 GTPase activating factor for Rsr1p/Bud1p; plays a role in spindle position checkpoint distinct from its role in bud site selection; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types mitotic cell cycle, organelle fission, cell budding, regulation of organelle organization, cytokinesis, proteolysis involved in cellular protein catabolic process, chromosome segregation, regulation of cell cycle enzyme regulator activity cellular bud, membrane, site of polarized growth, cytoplasm 4941 BY4741 11_1 18 C 3 YKL093W MBR1 - Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; MBR1 has a paralog, ISF1, that arose from the whole genome duplication generation of precursor metabolites and energy, cellular respiration molecular_function cellular_component 4942 BY4741 11_1 18 C 4 YKL094W YJU3 - Monoglyceride lipase (MGL); functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family lipid metabolic process hydrolase activity membrane, mitochondrial envelope, mitochondrion, plasma membrane, cytoplasm 4943 BY4741 11_1 18 C 5 YKL096W CWP1 YJU1 Cell wall mannoprotein that localizes to birth scars of daughter cells; linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; required for propionic acid resistance meiotic cell cycle, sporulation, cell wall organization or biogenesis structural molecule activity cell wall, membrane 4945 BY4741 11_2 18 C 6 YKL097C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species biological_process molecular_function cellular_component 4946 BY4741 11_2 18 C 7 YKL098W MTC2 - Protein of unknown function; mtc2 is synthetically sick with cdc13-1 biological_process molecular_function cellular_component 4948 BY4741 11_2 18 C 8 YKL100C YPF1 aspartic endopeptidase Intramembrane aspartyl protease of the perinuclear ER membrane; acts in a branch of ER-associated degradation (ERAD) that degrades functional proteins rather than misfolded proteins; regulates abundance of high-affinity plasma membrane transporters during the starvation response; has a presenilin fold; member of the GxGD family of intramembrane proteases; closest human homolog is signal peptide peptidase (SPP) proteolysis involved in cellular protein catabolic process, response to starvation peptidase activity, hydrolase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 4950 BY4741 11_2 18 C 9 YKL101W HSL1 protein kinase HSL1|ELM2|NIK1 Nim1p-related protein kinase; regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p peptidyl-amino acid modification, mitotic cell cycle, protein phosphorylation, regulation of cell cycle kinase activity, transferase activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 4951 BY4741 11_2 18 C 10 YKL102C - - Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site biological_process molecular_function cellular_component 4952 BY4741 11_2 18 C 11 YKL103C APE1 metalloaminopeptidase APE1|LAP4|API|YSC1 Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress other peptidase activity, hydrolase activity vacuole, cytoplasm 4953 BY4741 11_2 18 C 12 YKL105C SEG2 - Eisosome component; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes; SEG2 has a paralog, SEG1, that arose from the whole genome duplication biological_process molecular_function cellular_component 4955 BY4741 11_2 18 D 1 YKL106W AAT1 aspartate transaminase AAT1 Mitochondrial aspartate aminotransferase; catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis cellular amino acid metabolic process transferase activity mitochondrion, cytoplasm 4956 BY4741 11_2 18 D 2 YKL107W - putative short-chain dehydrogenase/reductase Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein biological_process molecular_function cellular_component 4957 BY4741 11_2 18 D 3 YKL109W HAP4 transcription factor HAP4 Transcription factor; subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex; involved in diauxic shift response to chemical, generation of precursor metabolites and energy, cellular respiration, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, transcription factor activity, protein binding, transcription factor binding nucleus 4959 BY4741 11_2 18 D 4 YKL110C KTI12 TOT4 Protein that plays a role in modification of tRNA wobble nucleosides; protein plays role in tRNA wobble nucleoside modification with Elongator complex; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p tRNA processing, transcription from RNA polymerase II promoter, RNA modification chromatin binding nucleus, cytoplasm 4960 BY4741 11_2 18 D 5 YKL113C RAD27 multifunctional nuclease RAD27|FEN1|RTH1|ERC11 5' to 3' exonuclease, 5' flap endonuclease; required for Okazaki fragment processing and maturation, for long-patch base-excision repair and large loop repair (LLR), ribonucleotide excision repair; member of the S. pombe RAD2/FEN1 family; relocalizes to the cytosol in response to hypoxia DNA repair, DNA replication, cellular response to DNA damage stimulus, RNA catabolic process, DNA recombination hydrolase activity, nuclease activity nucleus, mitochondrion, cytoplasm 4963 BY4741 11_2 18 D 6 YKL114C APN1 DNA-(apurinic or apyrimidinic site) lyase APN1 Major apurinic/apyrimidinic endonuclease; 3'-repair diesterase; involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine; genetically interacts with NTG1 to maintain mitochondrial genome integrity DNA repair, cellular response to DNA damage stimulus lyase activity, nuclease activity, hydrolase activity nucleus, mitochondrion, cytoplasm 4964 BY4741 11_2 18 D 7 YKL116C PRR1 serine/threonine protein kinase PRR1 Serine/threonine protein kinase; inhibits pheromone induced signaling downstream of MAPK, possibly at the level of the Ste12p transcription factor response to chemical, protein phosphorylation, conjugation, transcription from RNA polymerase II promoter kinase activity, transferase activity, signal transducer activity cytoplasm 4966 BY4741 11_2 18 D 8 YKL117W SBA1 Hsp90 cochaperone SBA1|CST18 Co-chaperone that binds and regulates Hsp90 family chaperones; plays a role in determining prion variants; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress telomere organization, DNA replication, regulation of organelle organization, protein folding, regulation of DNA metabolic process other nucleus, cytoplasm 4967 BY4741 11_2 18 D 9 YKL118W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPH2 biological_process molecular_function cellular_component 4968 BY4741 11_2 18 D 10 YKL119C VPH2 VMA12|CLS10 Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER); involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner cellular ion homeostasis, protein complex biogenesis molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 4969 BY4741 11_2 18 D 11 YKL120W OAC1 - Mitochondrial inner membrane transporter; transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 4970 BY4741 11_2 18 D 12 YKL121W DGR2 - Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds; DGR2 has a paralog, YMR102C, that arose from the whole genome duplication biological_process molecular_function cellular_component 4971 BY4741 11_2 18 E 1 YKL123W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SSH4 biological_process molecular_function cellular_component 4973 BY4741 11_2 18 E 2 YKL124W SSH4 MLF4 Specificity factor required for Rsp5p-dependent ubiquitination; also required for sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases protein targeting, endosomal transport, proteolysis involved in cellular protein catabolic process molecular_function vacuole, cytoplasm 4974 BY4741 11_2 18 E 3 YKL126W YPK1 serine/threonine protein kinase YPK1|SLI2 S/T protein kinase; phosphorylates, downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p by phosphorylation in response to compromised sphingolipid synthesis; involved in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6); mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; human homolog SGK1 can complement a null mutant; human homolog SGK2 can complement a ypk1 ypk2 double mutant response to chemical, protein phosphorylation, ion transport, regulation of transport, lipid transport, lipid metabolic process, conjugation kinase activity, transferase activity cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane 4976 BY4741 11_2 18 E 4 YKL127W PGM1 phosphoglucomutase PGM1 Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; PGM1 has a paralog, PGM2, that arose from the whole genome duplication carbohydrate metabolic process, generation of precursor metabolites and energy, oligosaccharide metabolic process isomerase activity cytoplasm 4977 BY4741 11_2 18 E 5 YKL128C PMU1 putative phosphomutase Putative phosphomutase; contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant biological_process molecular_function nucleus, cytoplasm 4978 BY4741 11_2 18 E 6 YKL129C MYO3 myosin 3 One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO3 has a paralog, MYO5, that arose from the whole genome duplication mitotic cell cycle, cell budding, cytokinesis, exocytosis, cell wall organization or biogenesis, response to osmotic stress, endocytosis hydrolase activity cell cortex, cytoskeleton, cytoplasm 4979 BY4741 11_2 18 E 7 YKL130C SHE2 - RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; binds to ER-derived membranes and targets mRNAs to cortical ER other RNA binding, lipid binding, mRNA binding endomembrane system, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm 4980 BY4741 11_2 18 E 8 YKL131W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4981 BY4741 11_2 18 E 9 YKL132C RMA1 putative tetrahydrofolate synthase Putative dihydrofolate synthetase; similar to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; RMA1 has a paralog, FOL3, that arose from the whole genome duplication biological_process ligase activity mitochondrion, cytoplasm 4982 BY4741 11_2 18 E 10 YKL133C - - Putative protein of unknown function; not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype); YKL133C has a paralog, MGR3, that arose from the whole genome duplication biological_process molecular_function cellular_component 4983 BY4741 11_2 18 E 11 YKL134C OCT1 - Mitochondrial intermediate peptidase; cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis protein targeting, mitochondrion organization, protein maturation, cellular ion homeostasis peptidase activity, hydrolase activity mitochondrion, cytoplasm 4984 BY4741 11_2 18 E 12 YKL135C APL2 - Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Golgi vesicle transport other membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm, Golgi apparatus 4985 BY4741 11_2 "AP! complex subunit B 1 adaptin -- growth on -met, super slow growth on -lys, super slow growth on drop in media, mates like alpha. Confirmed Alpha -- CORRECT STRAIN CAN BE FOUND IN PLATE 121 F8" 18 F 1 YKL136W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C biological_process molecular_function cellular_component 4986 BY4741 11_2 18 F 2 YKL137W CMC1 - Copper-binding protein of the mitochondrial intermembrane space; evolutionarily conserved; may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif mitochondrion organization, protein complex biogenesis ion binding membrane, mitochondrial envelope, mitochondrion, cytoplasm 4987 BY4741 11_2 18 F 3 YKL138C MRPL31 mitochondrial 54S ribosomal protein YmL31|YmL31 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4988 BY4741 11_2 18 F 4 YKL140W TGL1 YKL5 Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes lipid metabolic process hydrolase activity membrane 4990 BY4741 11_2 18 F 5 YKL142W MRP8 YKL3 Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis biological_process molecular_function cytoplasm 4992 BY4741 11_2 18 F 6 YKL143W LTV1 YKL2 Component of the GSE complex; GSE is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature response to chemical, ribosomal subunit export from nucleus, ribosomal small subunit biogenesis, response to oxidative stress, nuclear transport, response to osmotic stress molecular_function nucleus, endomembrane system, vacuole, membrane, cytoplasm 4993 BY4741 11_2 18 F 7 YKL146W AVT3 - Vacuolar transporter; exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters transmembrane transport, ion transport, amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm 4996 BY4741 11_2 18 F 8 YKL147C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 biological_process molecular_function cellular_component 4997 BY4741 11_2 18 F 9 YKL148C SDH1 succinate dehydrogenase flavoprotein subunit SDH1|SDHA Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits; mutations in human ortholog SDHA are associated with Leigh syndrome generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process oxidoreductase activity, ion binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 4998 BY4741 11_2 18 F 10 YKL149C DBR1 RNA lariat debranching enzyme|PRP26 RNA lariat debranching enzyme; catalyzes debranching of lariat introns formed during pre-mRNA splicing; required for efficient Ty1 transposition; knockdown of human homolog Dbr1 rescues toxicity of RNA-binding proteins TDP-43 and FUS which are implicated in amyotrophic lateral sclerosis (ALS), suggests potential therapeutic target for ALS and related TDP-43 proteinopathies; human homolog DBR1 can complement yeast dbr1 null mutant RNA catabolic process, RNA splicing, pseudohyphal growth, mRNA processing, transposition nuclease activity, hydrolase activity nucleus 4999 BY4741 11_2 18 F 11 YKL150W MCR1 - Mitochondrial NADH-cytochrome b5 reductase; involved in ergosterol biosynthesis response to chemical, response to oxidative stress, lipid metabolic process oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 5000 BY4741 11_2 18 F 12 YKL151C - NADHX dehydratase NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress cofactor metabolic process, nucleobase-containing small molecule metabolic process lyase activity cytoplasm 5001 BY4741 11_2 18 G 1 YPR151C SUE1 - Protein required for degradation of unstable forms of cytochrome c; located in the mitochondria other molecular_function mitochondrial envelope, mitochondrion, cytoplasm 7407 BY4741 00_18 18 G 2 YKL156W RPS27A ribosomal 40S subunit protein S27A|S27e|rp61|YS20|S27A Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation, rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 5006 BY4741 11_2 18 G 3 YKL157W APE2 YKL158W|LAP1 Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication other peptidase activity, hydrolase activity extracellular region, mitochondrion, cytoplasm 5007 BY4741 11_2 18 G 4 YKL158W extracellular region, mitochondrion, cytoplasm extracellular region, mitochondrion, cytoplasm extracellular region, mitochondrion, cytoplasm - - - 5008 BY4741 11_2 18 G 5 YKL159C RCN1 - Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region signaling enzyme binding, enzyme regulator activity cytoplasm 5009 BY4741 11_2 18 G 6 YKL160W ELF1 - Transcription elongation factor with a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression chromatin organization, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation enzyme binding nucleus 5010 BY4741 11_2 18 G 7 YKL161C KDX1 putative protein kinase KDX1|MLP1 Protein kinase; implicated in Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p; KDX1 has a paralog, SLT2, that arose from the whole genome duplication biological_process kinase activity, transferase activity cellular_component 5011 BY4741 11_2 18 G 8 YKL162C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion biological_process molecular_function mitochondrion, cytoplasm 5012 BY4741 11_2 18 G 9 YKL163W PIR3 CCW8 O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; coding sequence contains length polymorphisms in different strains; PIR3 has a paralog, HSP150, that arose from the whole genome duplication cell wall organization or biogenesis structural molecule activity cell wall 5013 BY4741 11_2 18 G 10 YKL164C PIR1 CCW6 O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication cell wall organization or biogenesis structural molecule activity cell wall, nucleus 5014 BY4741 11_2 18 G 11 YKL166C TPK3 cAMP-dependent protein kinase catalytic subunit TPK3 cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication protein phosphorylation, mitochondrion organization, signaling kinase activity, transferase activity nucleus, cytoplasm 5016 BY4741 11_2 18 G 12 YKL167C MRP49 mitochondrial 54S ribosomal protein MRP49 Mitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 5017 BY4741 11_2 18 H 1 YKL168C KKQ8 putative serine/threonine protein kinase KKQ8 Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication biological_process kinase activity, transferase activity cytoplasm 5018 BY4741 11_2 18 H 3 YKL169C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 biological_process molecular_function cellular_component 5019 BY4741 11_2 18 H 4 YKL170W MRPL38 mitochondrial 54S ribosomal protein YmL38/YmL34|YmL38|YmL34|MRPL34 Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 5020 BY4741 11_2 18 H 5 YKL171W NNK1 protein kinase NNK1 Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm protein phosphorylation kinase activity, transferase activity cytoplasm 5021 BY4741 11_2 18 H 6 YKL174C TPO5 - Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles other transmembrane transporter activity membrane, endomembrane system, cytoplasm, Golgi apparatus 5024 BY4741 11_2 18 H 7 YKL175W ZRT3 Zn(2+) transporter ZRT3 Vacuolar membrane zinc transporter; transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency cellular ion homeostasis, ion transport transmembrane transporter activity membrane, vacuole, cytoplasm 5025 BY4741 11_2 18 H 8 YKL176C LST4 - Subunit of the Lst4p-Lst7p GTPase activating protein complex for Gtr2p; stimulates the GTPase activity of Rag family GTPase Gtr2p, within the context of the Gtr1p-Gtr2p heterodimer, after amino acid stimulation; required for activation of TORC1 in response to amino acid stimulation; recruited to the vacuolar membrane during amino acid starvation and released from the membrane by TORC1; required for the transport of amino acid permease Gap1p from the Golgi to the cell surface signaling enzyme regulator activity membrane, vacuole, cytoplasm 5026 BY4741 11_2 18 H 9 YKL177W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene STE3 biological_process molecular_function cellular_component 5027 BY4741 11_2 18 H 10 YKL178C STE3 DAF2 Receptor for a factor pheromone; couples to MAP kinase cascade to mediate pheromone response; transcribed in alpha cells and required for mating by alpha cells, ligand bound receptors endocytosed and recycled to the plasma membrane; GPCR response to chemical, conjugation, signaling signal transducer activity membrane, plasma membrane 5028 BY4741 11_2 18 H 11 YKL179C COY1 - Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function Golgi vesicle transport molecular_function membrane, endomembrane system, cytoplasm, Golgi apparatus 5029 BY4741 11_2 18 H 12 YKL183W LOT5 - Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus, cytoplasm 5033 BY4741 11_2 19 A 1 YKL184W SPE1 ornithine decarboxylase SPE1|SPE10|ORD1 Ornithine decarboxylase; catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation cofactor metabolic process, vitamin metabolic process, monocarboxylic acid metabolic process lyase activity cytoplasm 5034 BY4741 11_2 19 A 2 YKL185W ASH1 DNA-binding transcription repressor ASH1 Component of the Rpd3L histone deacetylase complex; zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate mitotic cell cycle, pseudohyphal growth, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, regulation of cell cycle nucleic acid binding transcription factor activity, DNA binding cellular bud, nucleus, chromosome 5035 BY4741 11_2 19 A 3 YKL187C FAT3 - Protein required for fatty acid uptake; protein abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; FAT3 has a paralog, YLR413W, that arose from the whole genome duplication lipid transport, ion transport molecular_function membrane, mitochondrion, cytoplasm 5037 BY4741 11_2 19 A 4 YKL188C PXA2 ATP-binding cassette long-chain fatty acid transporter PXA2|PAT1 Subunit of heterodimeric peroxisomal ABC transport complex, with Pxa1p; required for import of long-chain fatty acids into peroxisomes; similar to human adrenoleukodystrophy transportesr ABCD1 and ABCD2, and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder; human ABCD1 and ABCD2 can each partially complement yeast pxa1 pxa2 double null mutant transmembrane transport, ion transport, lipid transport hydrolase activity, transmembrane transporter activity, ATPase activity peroxisome, membrane, cytoplasm 5038 BY4741 11_2 19 A 5 YKL190W CNB1 calcineurin regulatory subunit B|YCN2|CRV1 Calcineurin B; regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (stress-response transcription factor); other calcineurin subunit encoded by CNA1 and/or CMP1; regulates function of Aly1p alpha-arrestin; myristoylation by Nmt1p reduces calcineurin activity in response to submaximal Ca signals, is needed to prevent constitutive phosphatase activity; protein abundance increases in response to DNA replication stress response to chemical, cellular ion homeostasis, conjugation, cell wall organization or biogenesis, signaling phosphatase activity, hydrolase activity, ion binding other 5040 BY4741 11_2 19 A 6 YGR027C RPS25A ribosomal 40S subunit protein S25A|S25e|rp45|YS23|S31A|S25A|RPS31A Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 4657 BY4741 07_4 19 A 7 YGR031W IMO32 - Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 biological_process molecular_function mitochondrion, cytoplasm 4661 BY4741 07_4 19 A 8 YGR033C TIM21 FMP17 Nonessential component of the TIM23 complex; interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate the Translocase of the Inner Mitochondrial membrane (TIM23 complex) activity protein targeting, mitochondrion organization, transmembrane transport molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4663 BY4741 07_4 19 A 9 YGR034W RPL26B ribosomal 60S subunit protein L26B|L24|YL33|L33B|L26B Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 4664 BY4741 07_4 19 A 10 YGR035C - - Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; YGR035C has a paralog, YLR346C, that arose from the whole genome duplication biological_process molecular_function cellular_component 4665 BY4741 07_4 19 A 11 YGR036C CAX4 CWH8 Dolichyl pyrophosphate (Dol-P-P) phosphatase; has a luminally oriented active site in the ER; cleaves the anhydride linkage in Dol-P-P; required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation carbohydrate metabolic process, protein glycosylation, lipid metabolic process hydrolase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 4666 BY4741 07_4 19 A 12 YGR037C ACB1 long-chain fatty acid transporter ACB1 Acyl-CoA-binding protein; transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress lipid transport, ion transport ion binding nucleus, extracellular region, cytoplasm 4667 BY4741 07_4 19 B 1 YGR039W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722 biological_process molecular_function cellular_component 4669 BY4741 07_4 19 B 2 YGR041W BUD9 - Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole; BUD9 has a paralog, BUD8, that arose from the whole genome duplication mitotic cell cycle, cytokinesis molecular_function cellular bud, membrane, site of polarized growth, plasma membrane 4671 BY4741 07_4 19 B 3 YGR042W - - Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress biological_process molecular_function nucleus, cytoplasm 4672 BY4741 07_4 19 B 4 YGR043C NQM1 sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphate transaldolase NQM1 Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift; NQM1 has a paralog, TAL1, that arose from the whole genome duplication response to chemical, response to oxidative stress transferase activity nucleus 4673 BY4741 07_4 19 B 5 YGR044C RME1 CSP1 Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress mitotic cell cycle, meiotic cell cycle, organelle fission, invasive growth in response to glucose limitation, regulation of organelle organization, transcription from RNA polymerase II promoter, regulation of cell cycle nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 4674 BY4741 07_4 19 B 6 YGR045C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane 4675 BY4741 07_4 19 B 8 YGR049W SCM4 - Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation; SCM4 has a paralog, ATG33, that arose from the whole genome duplication biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4679 BY4741 07_4 19 B 9 YGR051C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene biological_process molecular_function cellular_component 4681 BY4741 07_4 19 B 10 YGR052W FMP48 protein kinase FMP48 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation protein phosphorylation kinase activity, transferase activity mitochondrion, cytoplasm 4682 BY4741 07_4 19 B 11 YGR054W - - Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A translational initiation RNA binding, translation factor activity, RNA binding, mRNA binding ribosome, cytoplasm 4684 BY4741 07_4 19 B 12 YGR055W MUP1 - High affinity methionine permease; integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake ion transport, amino acid transport transmembrane transporter activity membrane, plasma membrane 4685 BY4741 07_4 19 C 1 YGR056W RSC1 RSC subunit protein RSC1 Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook; RSC1 has a paralog, RSC2, that arose from the whole genome duplication DNA repair, chromatin organization, sporulation, cellular response to DNA damage stimulus, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation hydrolase activity, ATPase activity nucleus 4686 BY4741 07_4 19 C 2 YGR057C LST7 - Subunit of the Lst4p-Lst7p GTPase activating protein complex for Gtr2p; stimulates the GTPase activity of Rag family GTPase Gtr2p, within the context of the Gtr1p-Gtr2p heterodimer, after amino acid stimulation; required for activation of TORC1 in response to amino acid stimulation; recruited to the vacuolar membrane during amino acid starvation and released from the membrane by TORC1; required for the transport of amino acid permease Gap1p from the Golgi to the cell surface signaling enzyme regulator activity membrane, vacuole, cytoplasm 4687 BY4741 07_4 19 C 3 YGR058W PEF1 - Penta-EF-hand protein; required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly mitotic cell cycle, cell budding, regulation of organelle organization, cytokinesis, regulation of transport, vesicle organization, protein complex biogenesis, Golgi vesicle transport ion binding nucleus, cellular bud, site of polarized growth, cytoplasm 4688 BY4741 07_4 19 C 4 YGR059W SPR3 septin SPR3 Sporulation-specific homolog of the CDC3/10/11/12 family of genes; septin protein involved in sporulation; regulated by ABFI; the yeast CDC3/10/11/12 family is a family of bud neck microfilament genes meiotic cell cycle, sporulation structural molecule activity cell cortex, cytoskeleton, cell wall, membrane, cytoplasm 4689 BY4741 07_4 19 C 5 YGR061C ADE6 phosphoribosylformylglycinamidine synthase Formylglycinamidine-ribonucleotide (FGAM)-synthetase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway nucleobase-containing small molecule metabolic process ligase activity cytoplasm 4691 BY4741 07_4 19 C 6 YGR062C COX18 membrane insertase COX18|OXA2 Protein required for membrane insertion of C-terminus of Cox2p; mitochondrial integral inner membrane protein; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC; respiratory defect of the null mutant is functionally complemented by human COX18 carrying the N-terminal 54 amino acids of S. cerevisiae Cox18p mitochondrion organization protein transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 4692 BY4741 07_4 19 C 7 YGR064W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C other molecular_function cellular_component 4694 BY4741 07_4 19 C 8 YGR066C - - Putative protein of unknown function biological_process molecular_function cellular_component 4696 BY4741 07_4 19 C 9 YGR067C - - Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p biological_process DNA binding cellular_component 4697 BY4741 07_4 19 C 10 YGR068C ART5 - Protein proposed to regulate endocytosis of plasma membrane proteins; regulates by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane endocytosis enzyme binding cellular_component 4698 BY4741 07_4 19 C 11 YGR069W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4699 BY4741 07_4 19 C 12 YGR070W ROM1 Rho family guanine nucleotide exchange factor ROM1|SKC1 Guanine nucleotide exchange factor (GEF) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP; ROM1 has a paralog, ROM2, that arose from the whole genome duplication signaling guanyl-nucleotide exchange factor activity other 4700 BY4741 07_4 19 D 1 YGR071C ENV11 - Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; ENV11 has a paralog, VID22, that arose from the whole genome duplication vacuole organization, protein maturation molecular_function nucleus 4701 BY4741 07_4 19 D 2 YGR072W UPF3 SUP112|SUA6 Component of the nonsense-mediated mRNA decay (NMD) pathway; along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance RNA catabolic process, protein modification by small protein conjugation or removal, DNA recombination RNA binding, mRNA binding nucleus, nucleolus, cytoplasm 4702 BY4741 07_4 19 D 3 YGR076C MRPL25 mitochondrial 54S ribosomal protein YmL25|AFO1|YmL25|YMR26 Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4706 BY4741 07_4 19 D 4 YGR077C PEX8 PAS6 Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p protein targeting, transmembrane transport, peroxisome organization protein binding, bridging peroxisome, membrane, cytoplasm 4707 BY4741 07_4 19 D 5 YGR078C PAC10 tubulin-binding prefolding complex subunit PAC10|RKS2|PFD3|GIM2 Part of the heteromeric co-chaperone GimC/prefoldin complex; complex promotes efficient protein folding protein complex biogenesis cytoskeletal protein binding cytoplasm 4708 BY4741 07_4 19 D 6 YGR079W - - Putative protein of unknown function; YGR079W is not an essential gene biological_process molecular_function cellular_component 4709 BY4741 07_4 19 D 7 YGR080W TWF1 - Twinfilin; highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton; coordinates actin filament severing and monomer sequestering at sites of rapid actin turnover; composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly cytoskeleton organization, regulation of organelle organization, protein complex biogenesis cytoskeletal protein binding cell cortex, site of polarized growth, cytoskeleton, cytoplasm 4710 BY4741 07_4 19 D 8 YGR081C SLX9 - Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant rRNA processing, ribosomal subunit export from nucleus, nuclear transport, ribosomal small subunit biogenesis molecular_function nucleus, nucleolus 4711 BY4741 07_4 19 D 9 YGR084C MRP13 mitochondrial 37S ribosomal protein YmS-A Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 4714 BY4741 07_4 19 D 10 YGR085C RPL11B ribosomal 60S subunit protein L11B|rp39B|YL22|L5|L16A|L11B Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 4715 BY4741 07_4 19 D 11 YGR087C PDC6 indolepyruvate decarboxylase 6 Minor isoform of pyruvate decarboxylase; decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation cellular amino acid metabolic process lyase activity cytoplasm 4717 BY4741 07_4 19 D 12 YGR088W CTT1 catalase T|SPS101 Cytosolic catalase T; has a role in protection from oxidative damage by hydrogen peroxide response to chemical, response to oxidative stress, response to osmotic stress oxidoreductase activity cytoplasm 4718 BY4741 07_4 19 E 1 YGR096W TPC1 thiamine transporter TPC1 Mitochondrial membrane transporter; mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 4726 BY4741 07_4 19 E 2 YGR097W ASK10 RGC2 Regulator of the Fps1p glycerol channel; under nonstress conditions, binds to Fps1p to positively regulate glycerol transport; under osmotic stress, multiple phosphorylation by Hog1p causes Ask10p to dissociate from Fps1p; forms homodimers and heterodimerizes with paralog Rgc1p; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; associates with RNA polymerase II holoenzyme response to chemical, carbohydrate transport, regulation of transport, response to oxidative stress other nucleus, cytoplasm 4727 BY4741 07_4 19 E 3 YGR100W MDR1 GYP2|MIC1 Cytoplasmic GTPase-activating protein; activates Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function other enzyme regulator activity cytoplasm 4730 BY4741 07_4 19 E 4 YGR101W PCP1 rhomboid protease PCP1|RBD1|MDM37 Mitochondrial serine protease; required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases mitochondrion organization, regulation of organelle organization, protein maturation peptidase activity, hydrolase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 4731 BY4741 07_4 19 E 5 YGR102C GTF1 glutamyl-tRNA(Gln) amidotransferase subunit F Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 4732 BY4741 07_4 19 E 6 YGR104C SRB5 MED18 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance DNA-templated transcription, initiation, transcription from RNA polymerase II promoter, DNA-templated transcription, termination transcription factor activity, protein binding nucleus 4734 BY4741 07_4 19 E 7 YGR105W VMA21 - Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; diverged ortholog of human XMEA (X-linked Myopathy with Excessive Autophagy); functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) protein complex biogenesis molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 4735 BY4741 07_4 19 E 8 YGR107W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4737 BY4741 07_4 19 E 9 YGR108W CLB1 B-type cyclin CLB1|SCB1 B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB1 has a paralog, CLB2, that arose from the whole genome duplication mitotic cell cycle, meiotic cell cycle, protein phosphorylation, organelle fission, cytoskeleton organization, regulation of organelle organization, regulation of protein modification process, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm 4738 BY4741 07_4 19 E 10 YGR109C CLB6 B-type cyclin CLB6 B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1; CLB6 has a paralog, CLB5, that arose from the whole genome duplication mitotic cell cycle, meiotic cell cycle, regulation of protein modification process, protein phosphorylation, DNA replication, regulation of DNA metabolic process, regulation of cell cycle enzyme regulator activity cellular_component 4739 BY4741 07_4 19 E 11 YGR111W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus other molecular_function nucleus, cytoplasm 4741 BY4741 07_4 19 E 12 YGR112W SHY1 - Mitochondrial inner membrane protein required for complex IV assembly; associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome; complex IV is also known as cytochrome c oxidase mitochondrion organization, protein complex biogenesis unfolded protein binding membrane, mitochondrial envelope, mitochondrion, cytoplasm 4742 BY4741 07_4 19 F 1 YGR118W RPS23A ribosomal 40S subunit protein S23A|rp37|YS14|S28A|S23A|S12 Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal regulation of translation, rRNA processing, translational elongation, ribosomal small subunit biogenesis structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 4748 BY4741 07_4 19 F 2 YGR121C MEP1 ammonium permease MEP1|AMT1 Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation; human homolog RHCG complements yeast null mutant; mutations in human homolog RHCG implicated in metabolic acidosis; MEP1 has a paralog, MEP3, that arose from the whole genome duplication ion transport transmembrane transporter activity membrane, plasma membrane 4751 BY4741 07_4 19 F 3 YGR122W - - Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress RNA catabolic process, transcription from RNA polymerase II promoter molecular_function nucleus, membrane, plasma membrane, cytoplasm 4752 BY4741 07_4 19 F 4 YOR097C - - Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene biological_process molecular_function cellular_component 2353 BY4741 15_2 19 F 5 YOR099W KTR1 alpha-1,2-mannosyltransferase KTR1 Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress carbohydrate metabolic process, protein glycosylation transferase activity, transferring glycosyl groups, transferase activity endomembrane system, vacuole, membrane, Golgi apparatus, cytoplasm 2355 BY4741 15_2 19 F 6 YOR100C CRC1 carnitine:acyl carnitine antiporter Mitochondrial inner membrane carnitine transporter; required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation; human homolgy SLC25A20 complements yeast null mutant lipid metabolic process, monocarboxylic acid metabolic process transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 2356 BY4741 15_2 19 F 7 YOR101W RAS1 Ras family GTPase RAS1 GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress; RAS1 has a paralog, RAS2, that arose from the whole genome duplication nucleobase-containing small molecule metabolic process, signaling GTPase activity, hydrolase activity nucleus, membrane, cytoplasm, plasma membrane 2357 BY4741 15_2 19 F 8 YOR104W PIN2 - Exomer-dependent cargo protein; induces appearance of [PIN+] prion when overproduced; prion-like domain serves as a retention signal in the trans-Golgi network; predicted to be palmitoylated biological_process molecular_function cellular bud, membrane, site of polarized growth, plasma membrane 2360 BY4741 15_2 19 F 9 YOR105W - - Protein of unknown function; expressed at both mRNA and protein levels biological_process molecular_function cellular_component 2361 BY4741 15_2 19 F 10 YOR106W VAM3 SNAP receptor VAM3|PTH1 Syntaxin-like vacuolar t-SNARE; functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region vacuole organization, organelle fusion, vesicle organization, membrane fusion, membrane invagination other vacuole, membrane, cytoplasm 2362 BY4741 15_2 19 F 11 YOR107W RGS2 GTPase-activating protein RGS2 Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p signaling enzyme regulator activity nucleus, cytoplasm 2363 BY4741 15_2 19 F 12 YOR108W LEU9 2-isopropylmalate synthase LEU9 Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication cellular amino acid metabolic process transferase activity mitochondrion, cytoplasm 2364 BY4741 15_2 19 G 1 YOR109W INP53 phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP53|SOP2|SJL3 Polyphosphatidylinositol phosphatase; dephosphorylates multiple phosphatidylinositol phosphates; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains; INP53 has a paralog, INP52, that arose from the whole genome duplication lipid metabolic process phosphatase activity, hydrolase activity membrane, cell cortex, cytoskeleton, cytoplasm 2365 BY4741 15_2 19 G 2 YOR111W - - Putative protein of unknown function biological_process molecular_function cellular_component 2367 BY4741 15_2 19 G 3 YOR112W CEX1 - Component of nuclear aminoacylation-dependent tRNA export pathway; cytoplasmic; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a; required for activation of RAN GTPase Gsp1p and dissociation of receptor-tRNA-Gsp1p export complex; recruits Rna1p from cytoplasm to NPC, facilitates Rna1p activation of Gsp1p GTPase activity by enabling Rna1p to gain access to Gsp1p-GTP bound to export receptor tRNA complex nucleobase-containing compound transport, nuclear transport RNA binding nucleus, endomembrane system, cytoplasm 2368 BY4741 15_2 19 G 4 YOR113W AZF1 - Zinc-finger transcription factor; involved in diauxic shift; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity; relocalizes to the cytosol in response to hypoxia response to chemical, cell wall organization or biogenesis, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 2369 BY4741 15_2 19 G 5 YOR114W - - Putative protein of unknown function; null mutant is viable biological_process molecular_function cellular_component 2370 BY4741 15_2 19 G 6 YOR115C TRS33 - Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII) Golgi vesicle transport, protein complex biogenesis molecular_function endomembrane system, cytoplasm, Golgi apparatus 2371 BY4741 15_2 19 G 7 YOR118W RTC5 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity biological_process molecular_function cytoplasm 2374 BY4741 15_2 19 G 8 YOR120W GCY1 glycerol 2-dehydrogenase (NADP(+)) GCY1|GCY Glycerol dehydrogenase; involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; human homolog AKR1B1 can complement yeast null mutant; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication carbohydrate metabolic process, response to chemical, response to oxidative stress RNA binding, oxidoreductase activity, mRNA binding nucleus, cytoplasm 2376 BY4741 15_2 19 G 9 YOR121C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W biological_process molecular_function cellular_component 2377 BY4741 15_2 19 G 10 YOR123C LEO1 - Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay transcription from RNA polymerase I promoter, peptidyl-amino acid modification, chromatin organization, DNA-templated transcription, termination, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation, DNA repair, protein alkylation, regulation of organelle organization, cellular response to DNA damage stimulus, histone modification, regulation of protein modification process, protein modification by small protein conjugation or removal, regulation of DNA metabolic process RNA binding, ion binding, transcription factor activity, protein binding nucleus 2379 BY4741 15_2 19 G 11 YOR124C UBP2 ubiquitin-specific protease UBP2 Ubiquitin-specific protease; removes ubiquitin from ubiquitinated proteins; controls K63 homeostasis during oxidative stress; deubiquitinates Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process peptidase activity, hydrolase activity cytoplasm 2380 BY4741 15_2 19 G 12 YOR125C CAT5 putative monooxygenase CAT5|COQ7 Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation cofactor metabolic process oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 2381 BY4741 15_2 19 H 1 YOR126C IAH1 isoamyl acetate-hydrolyzing esterase Isoamyl acetate-hydrolyzing esterase; required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing monocarboxylic acid metabolic process hydrolase activity cellular_component 2382 BY4741 15_2 19 H 3 YOR127W RGA1 THE1|DBM1 GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; RGA1 has a paralog, RGA2, that arose from the whole genome duplication response to chemical, invasive growth in response to glucose limitation, cell budding, cytoskeleton organization, conjugation, pseudohyphal growth, signaling enzyme regulator activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 2383 BY4741 15_2 19 H 4 YOR129C AFI1 - Arf3p polarization-specific docking factor; required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p mitotic cell cycle, cytokinesis molecular_function nucleus, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane 2385 BY4741 15_2 19 H 5 YOR130C ORT1 ARG11 Ornithine transporter of the mitochondrial inner membrane; exports ornithine from mitochondria as part of arginine biosynthesis; functionally complemented by human ortholog, SLC25A15, which is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome, but HHH-associated variants fail to complement ion transport, cellular amino acid metabolic process, amino acid transport transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 2386 BY4741 15_2 19 H 6 YOR131C - putative haloacid dehalogenase-like hydrolase Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus, cytoplasm 2387 BY4741 15_2 19 H 7 YOR132W VPS17 retromer subunit VPS17|VPT3|PEP21 Subunit of the membrane-associated retromer complex; essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes endosomal transport protein transporter activity, ion binding, lipid binding membrane, endomembrane system, cytoplasm 2388 BY4741 15_2 19 H 8 YOR133W EFT1 elongation factor 2 Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication regulation of translation, translational elongation RNA binding, GTPase activity, hydrolase activity, translation factor activity, RNA binding ribosome, cytoplasm 2389 BY4741 15_2 19 H 9 YOR134W BAG7 - Rho GTPase activating protein (RhoGAP); stimulates the intrinsic GTPase activity of Rho1p, which plays a bud growth by regulating actin cytoskeleton organization and cell wall biosynthesis, resulting in the downregulation of Rho1p; structurally and functionally related to Sac7p; BAG7 has a paralog, SAC7, that arose from the whole genome duplication signaling enzyme regulator activity cellular_component 2390 BY4741 15_2 19 H 10 YOR135C IRC14 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci meiotic cell cycle, sporulation, DNA recombination, transcription from RNA polymerase II promoter molecular_function cellular_component 2391 BY4741 15_2 19 H 11 YOR136W IDH2 isocitrate dehydrogenase (NAD(+)) IDH2 Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; complex catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated generation of precursor metabolites and energy, cellular respiration, cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 2392 BY4741 15_2 19 H 12 YOR137C SIA1 - Protein of unassigned function; involved in activation of the Pma1p plasma membrane H+-ATPase by glucose; contains peptide signal for membrane localization ion transport molecular_function cellular_component 2393 BY4741 15_2 20 A 1 YOR138C RUP1 - Protein that regulates ubiquitination of Rsp5p; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress regulation of protein modification process, protein modification by small protein conjugation or removal other nucleus, cytoplasm 2394 BY4741 15_2 20 A 2 YOR139C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W biological_process molecular_function cellular_component 2395 BY4741 15_2 20 A 3 YOR140W SFL1 - Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p; premature stop codon (C1430T, Q477-stop) in SK1 background is linked to the aggressively invasive phenotype of SK1 relative to BY4741 (S288C) invasive growth in response to glucose limitation, pseudohyphal growth, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding, transcription factor binding nucleus 2396 BY4741 15_2 20 A 4 YOR141C ARP8 - Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes; has mRNA binding activity DNA repair, chromatin organization, cellular response to DNA damage stimulus, DNA recombination RNA binding, hydrolase activity, ATPase activity, helicase activity, mRNA binding nucleus, chromosome 2397 BY4741 15_2 20 A 5 YOR142W LSC1 succinate--CoA ligase (GDP-forming) subunit alpha Alpha subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration ligase activity mitochondrion, cytoplasm 2398 BY4741 15_2 20 A 6 YOR144C ELG1 RTT110 Subunit of an alternative replication factor C complex; important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication mitotic cell cycle, DNA repair, telomere organization, organelle fission, DNA replication, cellular response to DNA damage stimulus, transposition, chromosome segregation, DNA recombination, regulation of DNA metabolic process chromatin binding nucleus, chromosome, mitochondrion, cytoplasm 2400 BY4741 15_2 20 A 7 YOR152C ATG40 - Autophagy receptor with a role in endoplasmic reticulum degradation; involved specifically in autophagy of cortical and cytoplasmic ER in response to nitrogen starvation or rapamycin treatment; localizes to the cortical and cytoplasmic ER; similar to human FAM134B, which is also involved in ER autophagy and is associated with sensory neuropathy other protein binding, bridging membrane, cell cortex, endomembrane system, endoplasmic reticulum, cytoplasm 2408 BY4741 15_2 20 A 8 YOR153W PDR5 ATP-binding cassette multidrug transporter PDR5|STS1|YDR1|LEM1 Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter actively regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth; PDR5 has a paralog, PDR15, that arose from the whole genome duplication response to chemical, cellular ion homeostasis hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, cytoplasm, plasma membrane 2409 BY4741 15_2 20 A 9 YOR154W SLP1 - Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p protein folding molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 2410 BY4741 15_2 20 A 10 YOR155C ISN1 IMP 5'-nucleotidase Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase; catalyzes the breakdown of IMP to inosine; responsible for production of nicotinamide riboside and nicotinic acid riboside; expression positively regulated by nicotinic acid and glucose availability; does not show similarity to known 5'-nucleotidases from other organisms nucleobase-containing small molecule metabolic process phosphatase activity, hydrolase activity cellular_component 2411 BY4741 15_2 20 A 11 YOR156C NFI1 SUMO ligase NFI1|SIZ2 SUMO E3 ligase; catalyzes sumoylation of Yku70p/Yku80p and Sir4p promoting chromatin anchoring; DNA-bound form catalyzes a DNA-damaged triggered sumoylation wave resulting in multisite modification of several DNA repair proteins, enhancing interactions between these proteins and accelerating repair; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length; NFI1 has a paralog, SIZ1, that arose from the whole genome duplication peptidyl-amino acid modification, chromosome segregation, protein modification by small protein conjugation or removal transferase activity, DNA binding nucleus, chromosome, cytoplasm 2412 BY4741 15_2 20 A 12 YOR161C PNS1 - Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport biological_process molecular_function membrane, plasma membrane 2417 BY4741 15_2 20 B 1 YOR162C YRR1 PDR2 Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication response to chemical, transcription from RNA polymerase II promoter, response to oxidative stress nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 2418 BY4741 15_2 20 B 2 YOR163W DDP1 polyphosphatase DDP1 Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress carbohydrate metabolic process, nucleobase-containing small molecule metabolic process nuclease activity, hydrolase activity nucleus, cytoplasm 2419 BY4741 15_2 20 B 3 YOR164C GET4 ENV8 Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 protein targeting molecular_function cytoplasm 2420 BY4741 15_2 20 B 4 YOR165W SEY1 dynamin-like GTPase SEY1 Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 organelle fusion, membrane fusion, organelle inheritance GTPase activity, hydrolase activity cell cortex, endomembrane system, membrane, endoplasmic reticulum, cytoplasm 2421 BY4741 15_2 20 B 5 YOR166C SWT1 mRNA-processing endoribonuclease RNA endoribonuclease involved in perinuclear mRNP quality control; involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain RNA catabolic process nuclease activity, hydrolase activity nucleus, cytoplasm 2422 BY4741 15_2 20 B 6 YOR167C RPS28A ribosomal 40S subunit protein S28A|S28e|YS27|S33A|S28A|RPS33A Protein component of the small (40S) ribosomal subunit; has an extraribosomal function in regulation of RPS28B, in which Rps28Ap binds to a decapping complex via Edc3p, which then binds to RPS28B mRNA leading to its decapping and degradation; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28A has a paralog, RPS28B, that arose from the whole genome duplication cytoplasmic translation, nucleobase-containing compound transport, RNA catabolic process, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 2423 BY4741 15_2 20 B 7 YOR170W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4 biological_process molecular_function cellular_component 2426 BY4741 15_2 20 B 9 YOR171C LCB4 sphinganine kinase LCB4 Sphingoid long-chain base kinase; responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes; LCB4 has a paralog, LCB5, that arose from the whole genome duplication lipid metabolic process, signaling kinase activity, transferase activity cell cortex, endomembrane system, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm, plasma membrane 2427 BY4741 15_2 20 B 10 YOR172W YRM1 - Zinc finger transcription factor involved in multidrug resistance; Zn(2)-Cys(6) zinc finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 2428 BY4741 15_2 20 B 11 YOR173W DCS2 - m(7)GpppX pyrophosphatase regulator; non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance; DCS2 has a paralog, DCS1, that arose from the whole genome duplication RNA catabolic process hydrolase activity cytoplasm 2429 BY4741 15_2 20 B 12 YOR175C ALE1 lysophospholipid acyltransferase|LCA1|LPT1|SLC4 Broad-specificity lysophospholipid acyltransferase; part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids lipid metabolic process transferase activity membrane, endomembrane system, ribosome, endoplasmic reticulum, cytoplasm 2431 BY4741 15_2 20 C 1 YOR177C MPC54 - Component of the meiotic outer plaque; a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate meiotic cell cycle, sporulation, cell wall organization or biogenesis structural molecule activity cytoskeleton, microtubule organizing center 2433 BY4741 15_2 20 C 2 YOR178C GAC1 protein phosphatase regulator GAC1 Regulatory subunit for Glc7p type-1 protein phosphatase (PP1); tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock; GAC1 has a paralog, PIG1, that arose from the whole genome duplication response to heat, mitotic cell cycle, meiotic cell cycle, carbohydrate metabolic process, organelle fission, regulation of organelle organization, proteolysis involved in cellular protein catabolic process, generation of precursor metabolites and energy, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, regulation of cell cycle enzyme regulator activity cytoplasm 2434 BY4741 15_2 20 C 3 YOR182C RPS30B ribosomal 40S subunit protein S30B|S30e|S30B Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30B has a paralog, RPS30A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 2438 BY4741 15_2 20 C 4 YOR183W FYV12 - Protein of unknown function; required for survival upon exposure to K1 killer toxin biological_process molecular_function cellular_component 2439 BY4741 15_2 20 C 5 YOR184W SER1 O-phospho-L-serine:2-oxoglutarate transaminase|ADE9 3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process transferase activity cytoplasm 2440 BY4741 15_2 20 C 6 YOR185C GSP2 Ran GTPase GSP2|CNR2 GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); not required for viability; protein abundance increases in response to DNA replication stress; GSP2 has a paralog, GSP1, that arose from the whole genome duplication nucleus organization GTPase activity, hydrolase activity nucleus, membrane 2441 BY4741 15_2 20 C 7 YOR186W - - Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication biological_process molecular_function cellular_component 2442 BY4741 15_2 20 C 8 YOR187W TUF1 translation elongation factor Tu|tufM Mitochondrial translation elongation factor Tu (EF-Tu); involved in fundamental pathway of mtDNA homeostasis; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans; rare mutations in human mitochondrial elongation factor Tu (EFTu) associated with severe lactic acidosis, rapidly progressive fatal encephalopathy, severe infantile macrocystic leukodystrophy with micropolygyria mitochondrial translation, mitochondrion organization, translational elongation RNA binding, GTPase activity, hydrolase activity, translation factor activity, RNA binding mitochondrion, cytoplasm 2443 BY4741 15_2 20 C 9 YOR188W MSB1 - Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress other molecular_function cellular bud, membrane, site of polarized growth, mitochondrion, plasma membrane, cytoplasm 2444 BY4741 15_2 20 C 10 YOR189W IES4 - Component of the INO80 chromatiin remodeling complex; target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses telomere organization, DNA recombination, cellular response to DNA damage stimulus molecular_function nucleus, chromosome 2445 BY4741 15_2 20 C 11 YOR190W SPR1 SSG1 Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance; SPR1 has a paralog, EXG1, that arose from the whole genome duplication meiotic cell cycle, sporulation hydrolase activity, hydrolase activity, acting on glycosyl bonds cell wall, membrane 2446 BY4741 15_2 20 C 12 YOR191W ULS1 translocase ULS1|RIS1|TID4|DIS1 Swi2/Snf2-related translocase, SUMO-Targeted Ubiquitin Ligase (STUbL); required for maintenance of NHEJ inhibition at telomeres; functions at telomeres to translocate and ubiquitinylate poly-sumoylated Rap1p for proteosomal degradation; plays role in antagonizing silencing during mating-type switching; only known STUbL with a translocase activity; contains RING finger domain; relocalizes from nucleus to cytoplasm upon DNA replication stress DNA repair, chromatin organization, cellular response to DNA damage stimulus, regulation of DNA metabolic process hydrolase activity, ubiquitin-like protein binding, ATPase activity nucleus, mitochondrion, nucleolus, cytoplasm 2447 BY4741 15_2 20 D 1 YOR192C THI72 - Transporter of thiamine or related compound; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); shares sequence similarity with Thi7p transmembrane transport other membrane 2448 BY4741 15_2 20 D 2 YOR193W PEX27 - Peripheral peroxisomal membrane protein; involved in controlling peroxisome size and number, interacts with Pex25p; PEX27 has a paralog, PEX25, that arose from the whole genome duplication peroxisome organization molecular_function peroxisome, membrane, cytoplasm 2449 BY4741 15_3 20 D 3 YOR195W SLK19 - Kinetochore-associated protein; required for chromosome segregation and kinetochore clustering; required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate mitotic cell cycle, meiotic cell cycle, organelle fission, cytoskeleton organization, chromosome segregation molecular_function nucleus, cytoskeleton, chromosome 2451 BY4741 15_3 20 D 4 YOR196C LIP5 putative lipoate synthase Protein involved in biosynthesis of the coenzyme lipoic acid; has similarity to E. coli lipoic acid synthase other transferase activity, ligase activity mitochondrion, cytoplasm 2452 BY4741 15_3 20 D 5 YOR197W MCA1 Ca(2+)-dependent cysteine protease MCA1|YCA1 Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics and lifespan extension; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that block potential dimerization proteolysis involved in cellular protein catabolic process peptidase activity, hydrolase activity nucleus, cytoplasm 2453 BY4741 15_3 20 D 6 YOR198C BFR1 - Component of mRNP complexes associated with polyribosomes; involved in localization of mRNAs to P bodies; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity mitotic cell cycle, meiotic cell cycle, organelle fission, regulation of organelle organization, regulation of cell cycle RNA binding, mRNA binding endomembrane system, membrane, endoplasmic reticulum, cytoplasm 2454 BY4741 15_3 20 D 7 YOR199W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 2455 BY4741 15_3 20 D 8 YOR200W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c biological_process molecular_function cellular_component 2456 BY4741 15_3 20 D 9 YOR201C MRM1 PET56 Ribose methyltransferase; modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA rRNA processing, RNA modification transferase activity, methyltransferase activity mitochondrion, cytoplasm 2457 BY4741 15_3 20 D 10 YOR202W HIS3 imidazoleglycerol-phosphate dehydratase HIS3|HIS8|HIS10 Imidazoleglycerol-phosphate dehydratase; catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p cellular amino acid metabolic process lyase activity cellular_component 2458 BY4741 15_3 20 D 11 YOR205C GEP3 MTG3|AIM40|LRC5|FMP38 Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (Phb1p) rRNA processing, ribosomal small subunit biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 2461 BY4741 15_3 20 D 12 YOR208W PTP2 tyrosine protein phosphatase PTP2 Nuclear phosphotyrosine-specific phosphatase involved in osmosensing; involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; with Msg5p co-regulates the calcium signaling pathway protein phosphorylation, sporulation, protein dephosphorylation, regulation of protein modification process, cell wall organization or biogenesis, response to osmotic stress, signaling phosphatase activity, hydrolase activity nucleus 2464 BY4741 15_3 20 E 1 YOR209C NPT1 nicotinate phosphoribosyltransferase Nicotinate phosphoribosyltransferase; acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus cofactor metabolic process, chromatin organization, nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups, transferase activity nucleus 2465 BY4741 15_3 20 E 2 YOR211C MGM1 dynamin-related GTPase MGM1|MNA1 Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mitochondrial morphology, fusion, and genome maintenance; promotes membrane bending; exists as long and short form with different distributions; ratio of long to short forms is regulated by Psd1p; homolog of human OPA1 involved in autosomal dominant optic atrophy mitochondrion organization, organelle fusion, membrane fusion GTPase activity, hydrolase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 2467 BY4741 15_3 20 E 3 YOR212W STE4 G protein subunit beta|HMD2 G protein beta subunit; forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized growth site during mating; contains WD40 repeats response to chemical, invasive growth in response to glucose limitation, transposition, conjugation, signaling signal transducer activity membrane, plasma membrane 2468 BY4741 15_3 20 E 4 YOR213C SAS5 - Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity chromatin organization transferase activity nucleus, chromosome 2469 BY4741 15_3 20 E 5 YOR214C SPR2 - Putative spore wall protein; expression increases during sporulation; not an essential gene; YOR214C has a paralog, SPO19, that arose from the whole genome duplication biological_process molecular_function cell wall 2470 BY4741 15_3 20 E 6 YOR215C AIM41 - Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm 2471 BY4741 15_3 20 E 7 YOR216C RUD3 GRP1 Golgi matrix protein; involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Golgi vesicle transport enzyme binding membrane, endomembrane system, cytoplasm, Golgi apparatus 2472 BY4741 15_3 20 E 8 YOR219C STE13 YCI1 Dipeptidyl aminopeptidase; Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor protein maturation peptidase activity, hydrolase activity endomembrane system, cytoplasm, Golgi apparatus 2475 BY4741 15_3 20 E 9 YOR220W RCN2 WSP1 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress biological_process molecular_function cytoplasm 2476 BY4741 15_3 20 E 10 YOR221C MCT1 [acyl-carrier-protein] S-malonyltransferase Predicted malonyl-CoA:ACP transferase; putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling lipid metabolic process, monocarboxylic acid metabolic process transferase activity mitochondrion, cytoplasm 2477 BY4741 15_3 20 E 11 YOR222W ODC2 - Mitochondrial inner membrane transporter; 2-oxodicarboxylate transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism; ODC2 has a paralog, ODC1, that arose from the whole genome duplication other transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 2478 BY4741 15_3 20 E 12 YOR223W DSC3 - Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 biological_process molecular_function membrane, endomembrane system, vacuole, endoplasmic reticulum, cytoplasm 2479 BY4741 15_3 20 F 1 YOR225W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 2481 BY4741 15_3 20 F 2 YOR226C ISU2 putative iron-binding protein ISU2|NUA2 Mitochondrial protein required for iron-sulfur protein synthesis; performs scaffolding function during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; protein abundance increases under DNA replication stress; ISU2 has a paralog, ISU1, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant cellular ion homeostasis, tRNA processing, RNA modification molecular_function mitochondrion, cytoplasm 2482 BY4741 15_3 20 F 3 YOR227W HER1 - Protein of unknown function; required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication other molecular_function ribosome, mitochondrion, cytoplasm 2483 BY4741 15_3 20 F 4 YOR228C MCP1 - Mitochondrial protein of unknown function involved in lipid homeostasis; integral membrane protein that localizes to the mitochondrial outer membrane; involved in mitochondrial morphology; interacts genetically with MDM10, and other members of the ERMES complex; contains five predicted transmembrane domains mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 2484 BY4741 15_3 20 F 5 YOR229W WTM2 - Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats; relocalizes to the cytosol in response to hypoxia; WTM2 has a paralog, UME1, that arose from the whole genome duplication meiotic cell cycle, chromatin organization, regulation of organelle organization, cellular response to DNA damage stimulus, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, transcription factor activity, protein binding nucleus, cytoplasm 2485 BY4741 15_3 20 F 6 YOR230W WTM1 - Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats meiotic cell cycle, protein targeting, chromatin organization, regulation of organelle organization, nuclear transport, transcription from RNA polymerase II promoter transcription factor activity, protein binding nucleus 2486 BY4741 15_3 20 F 7 YOR231W MKK1 mitogen-activated protein kinase kinase MKK1|SSP32 MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication protein phosphorylation, peroxisome organization, cell wall organization or biogenesis, signaling kinase activity, transferase activity cellular bud, site of polarized growth, cytoplasm 2487 BY4741 15_3 20 F 8 YOR233W KIN4 putative serine/threonine protein kinase KIN4|KIN3|KIN31 Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication mitotic cell cycle, protein phosphorylation, organelle fission, regulation of organelle organization, proteolysis involved in cellular protein catabolic process, chromosome segregation, regulation of cell cycle kinase activity, transferase activity cell cortex, cytoskeleton, microtubule organizing center, cellular bud, site of polarized growth, cytoplasm 2489 BY4741 15_3 20 F 9 YOR234C RPL33B ribosomal 60S subunit protein L33B|L33e|rp47|Yl37|L37B|L33B|RPL37B Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 2490 BY4741 15_3 20 F 10 YOR235W IRC13 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci biological_process molecular_function cellular_component 2491 BY4741 15_3 20 F 11 YOR237W HES1 oxysterol-binding protein related protein HES1|OSH5 Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication lipid transport, membrane invagination, exocytosis, endocytosis lipid binding cellular_component 2493 BY4741 15_3 20 F 12 YOR238W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm 2494 BY4741 15_3 20 G 1 YOR239W ABP140 TRM140|YOR240W AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift cytoskeleton organization, tRNA processing, RNA modification protein binding, bridging, transferase activity, methyltransferase activity, cytoskeletal protein binding cell cortex, cytoskeleton, site of polarized growth, cytoplasm 2495 BY4741 15_3 20 G 2 YOR240W cell cortex, cytoskeleton, site of polarized growth, cytoplasm cell cortex, cytoskeleton, site of polarized growth, cytoplasm cell cortex, cytoskeleton, site of polarized growth, cytoplasm - - - 2496 BY4741 15_3 20 G 3 YOR241W MET7 tetrahydrofolate synthase|MET23 Folylpolyglutamate synthetase; catalyzes extension of the glutamate chains of the folate coenzymes, required for methionine synthesis and for maintenance of mitochondrial DNA; protein abundance increases in response to DNA replication stress other ligase activity mitochondrion, cytoplasm 2497 BY4741 15_3 20 G 4 YOR242C SSP2 - Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; expression controlled by a tightly regulated middle-meiotic promoter that is activated by Ndt80p; translation of SSP2 mRNA is delayed, such that the mRNA is present as nuclear divisions are taking place but is not engaged by ribosomes until relatively late in meiotic development meiotic cell cycle, protein phosphorylation, sporulation, regulation of protein modification process, cell wall organization or biogenesis molecular_function cell wall, membrane 2498 BY4741 15_3 20 G 5 YOR243C PUS7 pseudouridine synthase PUS7 Pseudouridine synthase; catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); also pseudouridylates some mRNAs; relocates from nucleus to cytoplasm during heat shock and differentially modifies some mRNAs during heat shock; conserved in archaea, vertebrates, and some bacteria tRNA processing, rRNA processing, RNA modification isomerase activity nucleus, cytoplasm 2499 BY4741 15_3 20 G 6 YOR245C DGA1 diacylglycerol O-acyltransferase Diacylglycerol acyltransferase; catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor; Lro1p and Dga1p can O-acylate ceramides; localized to lipid particles lipid metabolic process transferase activity membrane 2501 BY4741 15_3 20 G 7 YOR246C ENV9 - Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts vacuole organization, protein maturation oxidoreductase activity mitochondrion, cytoplasm 2502 BY4741 15_3 20 G 8 YOR247W SRL1 - Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication cell wall organization or biogenesis molecular_function cellular bud, cell wall, site of polarized growth, vacuole, cytoplasm 2503 BY4741 15_3 20 G 9 YOR248W - TOS11 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 2504 BY4741 15_3 20 G 10 YOR251C TUM1 - Rhodanese domain sulfur transferase; accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized tRNA processing, RNA modification transferase activity mitochondrion, cytoplasm 2507 BY4741 15_3 20 G 11 YOR252W TMA16 RBF17 Protein of unknown function that associates with ribosomes biological_process molecular_function nucleus, ribosome, cytoplasm 2508 BY4741 15_3 20 G 12 YOR253W NAT5 peptide alpha-N-acetyltransferase subunit NAT5|NAA50|ROG2|ARD2 Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing protein acylation transferase activity ribosome, cytoplasm 2509 BY4741 15_3 20 H 1 YOR255W OSW1 - Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function membrane 2511 BY4741 15_3 20 H 3 YOR258W HNT3 - DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress cellular response to DNA damage stimulus nuclease activity, hydrolase activity nucleus, cytoplasm 2514 BY4741 15_3 20 H 4 YOR263C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W biological_process molecular_function cellular_component 2519 BY4741 15_3 20 H 5 YOR264W DSE3 - Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress biological_process molecular_function cellular bud, nucleus, site of polarized growth, cytoplasm 2520 BY4741 15_3 20 H 6 YOR277C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 biological_process molecular_function cellular_component 2533 BY4741 15_3 20 H 7 YOR279C RFM1 - Component of the Sum1p-Rfm1p-Hst1p complex; Rfm1p tethers the Hst1p histone deacetylase to the DNA-binding protein Sum1p; complex is involved in transcriptional repression of middle sporulation genes and in initiation of DNA replication mitotic cell cycle, organelle fission, DNA replication, regulation of DNA metabolic process, transcription from RNA polymerase II promoter protein binding, bridging nucleus 2535 BY4741 15_3 20 H 8 YOR280C FSH3 - Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 biological_process molecular_function cellular_component 2536 BY4741 15_3 20 H 9 YOR283W - phosphoglycerate mutase Phosphatase with a broad substrate specificity; has some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene other phosphatase activity, hydrolase activity nucleus, cytoplasm 2539 BY4741 15_3 20 H 10 YOR284W HUA2 - Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly biological_process molecular_function cytoplasm 2540 BY4741 15_3 20 H 11 YOR285W RDL1 thiosulfate sulfurtransferase RDL1 Thiosulfate sulfurtransferase; contains a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress; similar to the human TSTD gene biological_process transferase activity membrane, endomembrane system, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm 2541 BY4741 15_3 20 H 12 YOR286W RDL2 thiosulfate sulfurtransferase RDL2|AIM42|FMP31 Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss biological_process transferase activity mitochondrion, cytoplasm 2542 BY4741 15_3 21 A 1 YOR288C MPD1 protein disulfide isomerase MPD1 Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation protein folding isomerase activity, oxidoreductase activity vacuole, cytoplasm 2544 BY4741 15_3 21 A 2 YJL218W - acetyltransferase Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene biological_process transferase activity cellular_component 1207 BY4741 10_1 21 A 3 YJL217W REE1 - Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle biological_process molecular_function cytoplasm 1208 BY4741 10_1 21 A 4 YJL216C IMA5 oligo-1,6-glucosidase IMA5 Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; most distant member of the IMA isomaltase family, but with similar catalytic properties as Ima1p and Ima2p; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose; can cleave alpha-1,3 linkage of nigerose and turanose and alpha-1,5 linkage of leucrose and is very sensitive to temperature in vitro carbohydrate metabolic process, oligosaccharide metabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds cellular_component 1209 BY4741 10_1 21 A 5 YJL215C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1210 BY4741 10_1 21 A 6 YJL214W HXT8 hexose transporter HXT8 Protein of unknown function with similarity to hexose transporters; expression is induced by low levels of glucose and repressed by high levels of glucose carbohydrate transport transmembrane transporter activity membrane, plasma membrane 1211 BY4741 10_1 21 A 7 YJL212C OPT1 oligopeptide transporter OPT1|GSH11|HGT1 Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family other transmembrane transporter activity membrane, plasma membrane 1213 BY4741 10_1 21 A 8 YJL210W PEX2 ubiquitin-protein ligase peroxin 2|PAS5|CRT1 RING-finger peroxin and E3 ubiquitin ligase; peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import protein targeting, protein modification by small protein conjugation or removal, transmembrane transport, peroxisome organization transferase activity peroxisome, membrane, cytoplasm 1214 BY4741 10_1 21 A 9 YJL211C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 biological_process molecular_function cellular_component 1215 BY4741 10_1 21 A 10 YJL209W CBP1 - Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress generation of precursor metabolites and energy, cellular respiration, RNA catabolic process RNA binding, mRNA binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 1216 BY4741 10_1 21 A 11 YJL208C NUC1 ribonuclease Major mitochondrial nuclease; has RNAse and DNA endo- and exonucleolytic activities; roles in mitochondrial recombination, apoptosis and maintenance of polyploidy; involved in fragmentation of genomic DNA during PND (programmed nuclear destruction); encodes ortholog of mammalian endoG RNA catabolic process, DNA recombination nuclease activity, hydrolase activity nucleus, membrane, mitochondrial envelope, mitochondrion, cytoplasm 1217 BY4741 10_1 21 A 12 YJL207C LAA1 - AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene endosomal transport molecular_function endomembrane system, ribosome, cytoplasmic membrane-bounded vesicle, membrane, Golgi apparatus, cytoplasm 1218 BY4741 10_1 21 B 1 YJL206C - - Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment biological_process molecular_function cellular_component 1219 BY4741 10_1 21 B 2 YJL206C-A cellular_component cellular_component cellular_component - - - 1220 BY4741 10_1 21 B 3 YJL204C RCY1 - F-box protein involved in recycling endocytosed proteins; involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth; direct interaction with C-terminal cytoplasmic region of Drs2p plays an important role for Drs2p function in endocytic recycling pathway Golgi vesicle transport, endosomal transport, endocytosis other site of polarized growth, endomembrane system, cytoplasm, Golgi apparatus 1221 BY4741 10_1 21 B 4 YJL201W ECM25 - Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p biological_process molecular_function cytoplasm 1224 BY4741 10_1 21 B 5 YJL200C ACO2 aconitate hydratase ACO2 Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol biological_process lyase activity mitochondrion, cytoplasm 1225 BY4741 10_1 21 B 6 YJL199C MBB1 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies biological_process molecular_function cellular_component 1226 BY4741 10_1 21 B 7 YJL198W PHO90 - Low-affinity phosphate transporter; acts upstream of Pho81p in regulation of the PHO pathway; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication transmembrane transport, ion transport, regulation of transport transmembrane transporter activity membrane, plasma membrane 1227 BY4741 10_1 21 B 8 YJL197W UBP12 putative ubiquitin-specific protease UBP12 Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm biological_process peptidase activity, hydrolase activity nucleus, cytoplasm 1228 BY4741 10_1 21 B 10 YJL196C ELO1 fatty acid elongase ELO1 Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, ELO2, that arose from the whole genome duplication lipid metabolic process, monocarboxylic acid metabolic process transferase activity membrane 1229 BY4741 10_1 21 B 11 YJL193W - - Putative protein of unknown function; predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect biological_process molecular_function membrane 1232 BY4741 10_1 21 B 12 YJL192C SOP4 EMC7 ER-membrane protein; subunit of evolutionarily conserved EMC (Endoplasmic Reticulum Membrane Complex) implicated in ERAD (ER-associated degradation) and proper assembly of multi-pass transmembrane (TM) proteins; EMC acts in yeast as an ER-mitochondria tether that interacts with outer membrane protein Tom5 of TOM (Translocase of the Mitochondrial Outer Membrane) complex; suppressor of pma1-7, deletion of SOP4 slows down export of wild-type Pma1p and Pma1-7 from the ER Golgi vesicle transport molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 1233 BY4741 10_1 21 C 1 YJL191W RPS14B ribosomal 40S subunit protein S14B|S11|rp59B|S14B|CRY2 Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly RNA binding, structural constituent of ribosome, rRNA binding, structural molecule activity ribosome, cytoplasm 1234 BY4741 10_1 21 C 2 YJL190C RPS22A ribosomal 40S subunit protein S22A|S8|rp50|YS22|S24A|S22A|RPS24 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 1235 BY4741 10_1 21 C 3 YJL189W RPL39 ribosomal 60S subunit protein L39|L39e|YL40|L46|L39|SPB2|RPL46 Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 1236 BY4741 10_1 21 C 4 YJL188C BUD19 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay mitotic cell cycle, cytokinesis molecular_function cellular_component 1237 BY4741 10_1 21 C 5 YJL187C SWE1 tyrosine protein kinase SWE1|WEE1 Protein kinase that regulates the G2/M transition; negative regulator of the Cdc28p kinase; morphogenesis checkpoint kinase; positive regulator of sphingolipid biosynthesis via Orm2p; phosphorylates a tyrosine residue in the N-terminus of Hsp90 in a cell-cycle associated manner, thus modulating the ability of Hsp90 to chaperone a selected clientele; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate mitotic cell cycle, meiotic cell cycle, protein phosphorylation, organelle fission, cytoskeleton organization, regulation of organelle organization, regulation of protein modification process, regulation of cell cycle kinase activity, transferase activity nucleus, cellular bud, site of polarized growth 1238 BY4741 10_1 21 C 6 YJL186W MNN5 alpha-1,2-mannosyltransferase MNN5 Alpha-1,2-mannosyltransferase; responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups, transferase activity endomembrane system, cytoplasm, Golgi apparatus 1239 BY4741 10_1 21 C 7 YJL185C ATG36 - Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene peroxisome organization molecular_function peroxisome, cytoplasm 1240 BY4741 10_1 21 C 8 YJL183W MNN11 alpha-1,6-mannosyltransferase Subunit of a Golgi mannosyltransferase complex; this complex also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p carbohydrate metabolic process, protein glycosylation transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, Golgi apparatus, cytoplasm 1242 BY4741 10_1 21 C 9 YJL181W - RBH1 Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication biological_process molecular_function cellular_component 1243 BY4741 10_1 21 C 10 YJL182C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W biological_process molecular_function cellular_component 1244 BY4741 10_1 21 C 11 YJL180C ATP12 ATP synthase complex assembly protein ATP12 Assembly factor for F1 sector of mitochondrial F1F0 ATP synthase; conserved protein; required for assembly of alpha and beta subunits into F1 sector of mitochondrial F1F0 ATP synthase; human homolog ATPAF2 can complement yeast atp12 mutant; mutation of human homolog reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency mitochondrion organization, protein complex biogenesis other mitochondrion, cytoplasm 1245 BY4741 10_1 21 C 12 YJL179W PFD1 prefolding complex chaperone subunit|GIM6 Subunit of heterohexameric prefoldin; prefoldin binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation protein folding, cytoskeleton organization, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation unfolded protein binding other 1246 BY4741 10_1 21 D 1 YJL178C ATG27 ETF1 Type I membrane protein involved in autophagy and the Cvt pathway; may be involved in membrane delivery to the phagophore assembly site vacuole organization, protein targeting, organelle assembly, vesicle organization, peroxisome organization lipid binding, ion binding endomembrane system, mitochondrion, membrane, Golgi apparatus, cytoplasm 1247 BY4741 10_1 21 D 2 YJL176C SWI3 HAF2|TYE2 Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; contains SANT domain that is required for SWI/SNF assembly; is essential for displacement of histone H2A-H2B dimers during ATP-dependent remodeling; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions carbohydrate metabolic process, response to chemical, chromatin organization, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, oligosaccharide metabolic process histone binding, hydrolase activity, DNA binding, ATPase activity nucleus, cytoplasm 1250 BY4741 10_1 21 D 3 YJL172W CPS1 - Vacuolar carboxypeptidase S; expression is induced under low-nitrogen conditions proteolysis involved in cellular protein catabolic process peptidase activity, hydrolase activity vacuole, cytoplasm 1253 BY4741 10_1 21 D 4 YJL171C - - GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress biological_process molecular_function cell wall, membrane, mitochondrion, plasma membrane, cytoplasm 1254 BY4741 10_1 21 D 5 YJL170C ASG7 - Protein that regulates signaling from G protein beta subunit Ste4p; contributes to relocalization of Ste4p within the cell; specific to a-cells and induced by alpha-factor conjugation molecular_function membrane, plasma membrane 1255 BY4741 10_1 21 D 6 YJL169W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 biological_process molecular_function cellular_component 1256 BY4741 10_1 21 D 7 YJL168C SET2 histone methyltransferase SET2|KMT3|EZL1 Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36 (H3K36), which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain(CTD) of Rpo21p; H3K36me3 (trimethylation) requires Spt6p, proline 38 on H3, CTD of Rpo21p, Ctk1p, and C-terminal SRI domain of Ste2p; relocalizes to the cytosol in response to hypoxia peptidyl-amino acid modification, meiotic cell cycle, protein acylation, organelle fission, chromatin organization, DNA replication, DNA-templated transcription, termination, DNA recombination, DNA-templated transcription, elongation, protein alkylation, sporulation, regulation of organelle organization, histone modification, regulation of protein modification process, regulation of DNA metabolic process, regulation of cell cycle methyltransferase activity, transferase activity nucleus, cytoplasm 1257 BY4741 10_1 21 D 8 YJL166W QCR8 ubiquinol--cytochrome-c reductase subunit 8|COR5 Subunit 8 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 1259 BY4741 10_1 21 D 9 YJL165C HAL5 protein kinase HAL5 Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication cellular ion homeostasis, protein phosphorylation, protein dephosphorylation kinase activity, transferase activity cellular_component 1260 BY4741 10_1 21 D 10 YJL164C TPK1 cAMP-dependent protein kinase catalytic subunit TPK1|SRA3|PKA1 cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; phosphorylates and inhibits Whi3p to promote G1/S phase passage; partially redundant with Tpk2p and Tpk3p; phosphorylates pre-Tom40p, which impairs its import into mitochondria under non-respiratory conditions; TPK1 has a paralog, TPK3, that arose from the whole genome duplication protein phosphorylation, signaling kinase activity, transferase activity nucleus, chromosome, cytoplasm 1261 BY4741 10_1 21 D 11 YJL163C - - Putative protein of unknown function biological_process molecular_function membrane 1262 BY4741 10_1 21 D 12 YJL162C JJJ2 - Protein of unknown function; contains a J-domain, which is a region with homology to the E. coli DnaJ protein biological_process molecular_function nucleus, cytoplasm 1263 BY4741 10_1 21 E 1 YJL161W FMP33 - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 1264 BY4741 10_1 21 E 2 YJL159W HSP150 heat shock protein HSP150|PIR2|ORE1|CCW7 O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication cell wall organization or biogenesis structural molecule activity, ATPase activity, hydrolase activity cell wall, extracellular region 1266 BY4741 10_1 21 E 3 YJL158C CIS3 SCW8|CCW5|PIR4|CCW11 Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family cell wall organization or biogenesis structural molecule activity endomembrane system, cell wall, cellular bud, extracellular region, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm, plasma membrane 1267 BY4741 10_1 21 E 4 YJL157C FAR1 cyclin-dependent protein serine/threonine kinase inhibiting protein FAR1 CDK inhibitor and nuclear anchor; during the cell cycle Far1p sequesters the GEF Cdc24p in the nucleus; phosphorylation by Cdc28p-Cln results in SCFCdc4 complex-mediated ubiquitin-dependent degradation, releasing Cdc24p for export and activation of GTPase Cdc42p; in response to pheromone, phosphorylation of Far1p by MAPK Fus3p results in association with, and inhibition of Cdc28p-Cln, as well as Msn5p mediated nuclear export of Far1p-Cdc24p, targeting Cdc24p to polarity sites response to chemical, conjugation, signaling, regulation of cell cycle enzyme regulator activity nucleus, membrane, site of polarized growth, cytoplasm 1268 BY4741 10_1 21 E 5 YJL155C FBP26 fructose-2,6-bisphosphatase Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress carbohydrate metabolic process phosphatase activity, hydrolase activity cytoplasm 1270 BY4741 10_1 21 E 6 YJL154C VPS35 retromer subunit VPS35|VPT7|GRD9 Endosomal subunit of membrane-associated retromer complex; required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p; overexpression of wild-type human VPS35 or Parkinson's-associated vps35-D686N or vps35-P299S variants complements Ni2+ resistance and Cd2+ sensitivity of yeast vps35 null mutant endosomal transport protein transporter activity membrane, endomembrane system, vacuole, cytoplasm 1271 BY4741 10_1 21 E 7 YJL153C INO1 inositol-3-phosphate synthase INO1|APR1 Inositol-3-phosphate synthase; involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element carbohydrate metabolic process isomerase activity cytoplasm 1272 BY4741 10_1 21 E 8 YJL152W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1273 BY4741 10_1 21 E 9 YJL151C SNA3 - Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles endosomal transport enzyme binding membrane, vacuole, cytoplasm 1274 BY4741 10_1 21 E 10 YJL150W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 1275 BY4741 10_1 21 E 11 YJL149W DAS1 SCF ubiquitin ligase complex subunit DAS1 Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C proteolysis involved in cellular protein catabolic process protein binding, bridging, transferase activity other 1276 BY4741 10_1 21 E 12 YJL148W RPA34 DNA-directed RNA polymerase I subunit RPA34|CST21|A34.5 RNA polymerase I subunit A34.5; essential for nucleolar assembly and for high polymerase loading rate; nucleolar localization depends on Rpa49p transcription from RNA polymerase I promoter, DNA-templated transcription, elongation transferase activity, nucleotidyltransferase activity nucleus, nucleolus 1277 BY4741 10_1 21 F 1 YJL147C MRX5 - Protein that associates with mitochondrial ribosome; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene biological_process molecular_function mitochondrion, cytoplasm 1278 BY4741 10_1 21 F 2 YJL146W IDS2 - Protein involved in modulation of Ime2p activity during meiosis; appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation meiotic cell cycle, organelle fission molecular_function nucleus, cytoplasm 1279 BY4741 10_1 21 F 3 YJL145W SFH5 - Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress ion transport, regulation of transport, Golgi vesicle transport, lipid transport, lipid metabolic process, exocytosis other nucleus, cell cortex, endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane 1280 BY4741 10_1 21 F 4 YJL144W - - Cytoplasmic hydrophilin essential in desiccation-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress response to chemical molecular_function cytoplasm 1281 BY4741 10_1 21 F 5 YJL142C IRC9 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci DNA recombination molecular_function membrane 1283 BY4741 10_1 21 F 6 YJL140W RPB4 DNA-directed RNA polymerase II subunit RPB4|B32|CTF15 RNA polymerase II subunit B32; forms dissociable heterodimer with Rpb7p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNAPII complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation response to heat, translational initiation, regulation of translation, nucleobase-containing compound transport, RNA catabolic process, DNA-templated transcription, initiation, mRNA processing, transcription from RNA polymerase II promoter, nuclear transport RNA binding, transferase activity, nucleotidyltransferase activity, DNA binding nucleus, cytoplasm 1285 BY4741 10_1 21 F 7 YJL139C YUR1 mannosyltransferase YUR1 Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication carbohydrate metabolic process, protein glycosylation, cell wall organization or biogenesis transferase activity, transferring glycosyl groups, transferase activity endomembrane system, Golgi apparatus, cytoplasm 1286 BY4741 10_1 21 F 8 YJL138C TIF2 translation initiation factor eIF4A|eIF4A Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication translational initiation, regulation of translation RNA binding, hydrolase activity, translation factor activity, RNA binding, ATPase activity, helicase activity ribosome, membrane, cytoplasm, plasma membrane 1287 BY4741 10_1 21 F 9 YJL135W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 biological_process molecular_function cellular_component 1290 BY4741 10_1 21 F 10 YJL134W LCB3 sphinganine kinase LCB3|YSR2|LBP1 Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication lipid metabolic process, signaling phosphatase activity, hydrolase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 1291 BY4741 10_1 21 F 11 YJL133W MRS3 - Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication RNA splicing, ion transport transmembrane transporter activity membrane, mitochondrion, cytoplasm 1292 BY4741 10_1 21 F 12 YJL132W - - Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene biological_process molecular_function membrane 1293 BY4741 10_1 21 G 1 YJL131C AIM23 - Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss translational initiation, mitochondrial translation, mitochondrion organization RNA binding, translation factor activity, RNA binding mitochondrion, cytoplasm 1294 BY4741 10_1 21 G 2 YJL130C URA2 bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase; catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process transferase activity, ligase activity membrane, mitochondrion, cytoplasm 1295 BY4741 10_1 21 G 3 YJL129C TRK1 - Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication cellular ion homeostasis transmembrane transporter activity membrane, plasma membrane 1296 BY4741 10_1 21 G 4 YLR324W PEX30 - Peroxisomal integral membrane protein; involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p; PEX30 has a paralog, PEX31, that arose from the whole genome duplication peroxisome organization molecular_function peroxisome, membrane, cytoplasm 5233 BY4741 12_5 21 G 5 YLR325C RPL38 ribosomal 60S subunit protein L38|L38e|L38 Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 5234 BY4741 12_5 21 G 6 YLR326W - - Putative protein of unknown function; predicted to be palmitoylated biological_process molecular_function cellular_component 5235 BY4741 12_5 21 G 7 YLR327C TMA10 RBF9|SFL2 Protein of unknown function that associates with ribosomes; protein abundance increases in response to DNA replication stress; TMA10 has a paralog, STF2, that arose from the whole genome duplication biological_process molecular_function nucleus, ribosome, cytoplasm 5236 BY4741 12_5 21 G 8 YLR328W NMA1 nicotinamide-nucleotide adenylyltransferase NMA1 Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; homolog of human NMNAT; NMA1 has a paralog, NMA2, that arose from the whole genome duplication cofactor metabolic process, nucleobase-containing small molecule metabolic process transferase activity, nucleotidyltransferase activity nucleus, cytoplasm 5237 BY4741 12_5 21 G 9 YLR329W REC102 - Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination meiotic cell cycle, organelle fission, DNA recombination molecular_function nucleus, chromosome 5238 BY4741 12_5 21 G 10 YLR330W CHS5 CAL3 Component of the exomer complex; exomer also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane; Chs5p is the only protein with a BRCT domain that is not localized to the nucleus mitotic cell cycle, meiotic cell cycle, carbohydrate metabolic process, sporulation, cytokinesis, Golgi vesicle transport, conjugation, cell wall organization or biogenesis molecular_function endomembrane system, cytoplasmic membrane-bounded vesicle, site of polarized growth, Golgi apparatus, cytoplasm 5239 BY4741 12_5 21 G 11 YLR331C JIP3 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 biological_process molecular_function cellular_component 5240 BY4741 12_5 21 G 12 YLR332W MID2 KAI1 O-glycosylated plasma membrane protein; acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p; MID2 has a paralog, MTL1, that arose from the whole genome duplication response to chemical, RNA splicing, cell morphogenesis, peroxisome organization, conjugation, cell wall organization or biogenesis, mRNA processing, signaling, response to osmotic stress signal transducer activity membrane, site of polarized growth, plasma membrane 5241 BY4741 12_5 21 H 1 YLR333C RPS25B ribosomal 40S subunit protein S25B|S25e|rp45|YS23|S31B|S25B Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25B has a paralog, RPS25A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 5242 BY4741 12_5 21 H 3 YLR335W NUP2 nucleoporin NUP2 Nucleoporin involved in nucleocytoplasmic transport; binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization response to heat, protein targeting, chromatin organization, nucleobase-containing compound transport, nuclear transport, nucleus organization enzyme binding nucleus, endomembrane system, mitochondrion, membrane, cytoplasm 5244 BY4741 12_5 21 H 4 YLR341W SPO77 - Meiosis-specific protein of unknown function; required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function other 5250 BY4741 12_5 21 H 5 YLR342W FKS1 1,3-beta-D-glucan synthase|PBR1|GSC1|ETG1|CWH53|CND1 Catalytic subunit of 1,3-beta-D-glucan synthase; functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling; FKS1 has a paralog, GSC2, that arose from the whole genome duplication carbohydrate metabolic process, regulation of transport, endocytosis transferase activity, transferring glycosyl groups, transferase activity cell cortex, cytoskeleton, mitochondrion, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane 5251 BY4741 12_5 21 H 6 YLR344W RPL26A ribosomal 60S subunit protein L26A|L24|YL33|L33A|L26A Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 5253 BY4741 12_5 21 H 7 YLR345W - bifunctional fructose-2,6-bisphosphate 2-phosphatase/6-phosphofructo-2-kinase Similar to 6-phosphofructo-2-kinase enzymes; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process kinase activity, transferase activity cytoplasm 5254 BY4741 12_5 21 H 8 YLR348C DIC1 - Mitochondrial dicarboxylate carrier; integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 5257 BY4741 12_5 21 H 9 YLR349W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C biological_process molecular_function cellular_component 5258 BY4741 12_5 21 H 10 YLR350W ORM2 sphingolipid homeostasis protein ORM2 Protein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress; ORM2 has a paralog, ORM1, that arose from the whole genome duplication response to chemical, lipid metabolic process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 5259 BY4741 12_5 21 H 11 YLR351C NIT3 putative hydolase Nit protein; one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member biological_process hydrolase activity mitochondrion, cytoplasm 5260 BY4741 12_5 21 H 12 YLR352W - - Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene biological_process molecular_function other 5261 BY4741 12_5 22 A 1 YLR353W BUD8 - Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole; BUD8 has a paralog, BUD9, that arose from the whole genome duplication mitotic cell cycle, cytokinesis, pseudohyphal growth molecular_function cellular bud, membrane, site of polarized growth, plasma membrane 5262 BY4741 12_5 22 A 2 YLR354C TAL1 sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphate transaldolase TAL1 Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate; TAL1 has a paralog, NQM1, that arose from the whole genome duplication cofactor metabolic process, nucleobase-containing small molecule metabolic process transferase activity nucleus, cytoplasm 5263 BY4741 12_5 22 A 3 YLR356W ATG33 - Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes; ATG33 has a paralog, SCM4, that arose from the whole genome duplication mitochondrion organization molecular_function membrane, mitochondrion, cytoplasm 5265 BY4741 12_5 22 A 4 YLR357W RSC2 - Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance; RSC2 has a paralog, RSC1, that arose from the whole genome duplication DNA repair, chromatin organization, cellular response to DNA damage stimulus, chromosome segregation, DNA recombination, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation hydrolase activity, ATPase activity nucleus 5266 BY4741 12_5 22 A 5 YLR360W VPS38 VPL17 Part of a Vps34p phosphatidylinositol 3-kinase complex; functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated endosomal transport molecular_function membrane, vacuole, cytoplasm 5269 BY4741 12_5 22 A 6 YLR362W STE11 mitogen-activated protein kinase kinase kinase STE11 Signal transducing MEK kinase; involved in pheromone response and pseudohyphal/invasive growth pathways where it phosphorylates Ste7p, and the high osmolarity response pathway, via phosphorylation of Pbs2p; regulated by Ste20p and Ste50p; protein abundance increases in response to DNA replication stress response to heat, response to chemical, protein phosphorylation, invasive growth in response to glucose limitation, transposition, regulation of protein modification process, conjugation, pseudohyphal growth, cell wall organization or biogenesis, signaling, response to osmotic stress kinase activity, transferase activity, signal transducer activity cytoplasm 5271 BY4741 12_5 22 A 7 YLR363C NMD4 - Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress RNA catabolic process molecular_function nucleus, cytoplasm 5272 BY4741 12_5 22 A 8 YLR364W GRX8 glutathione-disulfide reductase GRX8 Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic biological_process oxidoreductase activity cytoplasm 5273 BY4741 12_5 22 A 9 YLR365W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps dubious gene YLR364C-A; YLR365W is not an essential gene biological_process molecular_function cellular_component 5274 BY4741 12_5 22 A 10 YLR366W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A biological_process molecular_function cellular_component 5275 BY4741 12_5 22 A 11 YLR367W RPS22B ribosomal 40S subunit protein S22B|S8|rp50|YS22|S24B|S22B Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 5276 BY4741 12_5 22 A 12 YLR368W MDM30 SCF ubiquitin ligase complex subunit MDM30|DSG1 F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains mitochondrion organization, regulation of transport, organelle fusion, proteolysis involved in cellular protein catabolic process, nucleobase-containing compound transport, membrane fusion, protein modification by small protein conjugation or removal, nuclear transport transferase activity mitochondrion, cytoplasm 5277 BY4741 12_5 22 B 1 YLR371W ROM2 Rho family guanine nucleotide exchange factor ROM2 Guanine nucleotide exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p; ROM2 has a paralog, ROM1, that arose from the whole genome duplication regulation of transport, cell wall organization or biogenesis, signaling, endocytosis guanyl-nucleotide exchange factor activity, lipid binding, ion binding, signal transducer activity cellular bud, site of polarized growth 5280 BY4741 12_5 22 B 2 YLR372W ELO3 SUR4|fatty acid elongase ELO3|APA1|VBM1|SRE1 Elongase; involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis; lethality of the elo2 elo3 double null mutation is functionally complemented by human ELOVL1 and weakly complemented by human ELOVL3 or ELOV7 monocarboxylic acid metabolic process, Golgi vesicle transport, lipid metabolic process transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 5281 BY4741 12_5 22 B 3 YLR373C VID22 - Glycosylated integral membrane protein localized to plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles; VID22 has a paralog, ENV11, that arose from the whole genome duplication other molecular_function nucleus, membrane, plasma membrane 5282 BY4741 12_5 22 B 4 YLR374C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W biological_process molecular_function cellular_component 5283 BY4741 12_5 22 B 5 YLR375W STP3 - Zinc-finger protein of unknown function; possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids; STP3 has a paralog, STP4, that arose from the whole genome duplication biological_process DNA binding nucleus 5284 BY4741 12_5 22 B 6 YLR376C PSY3 - Component of Shu complex (aka PCSS complex); Shu complex also includes Shu1, Csm2, Shu2, and promotes error-free DNA repair; promotes Rad51p filament assembly; Shu complex mediates inhibition of Srs2p function; Psy3p and Csm2p contain similar DNA-binding regions which work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C DNA repair, DNA recombination, cellular response to DNA damage stimulus molecular_function nucleus, chromosome, cytoplasm 5285 BY4741 12_5 22 B 7 YLR377C FBP1 fructose 1,6-bisphosphate 1-phosphatase|ACN8 Fructose-1,6-bisphosphatase; key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; glucose starvation results in redistribution to the periplasm; interacts with Vid30p carbohydrate metabolic process phosphatase activity, hydrolase activity cytoplasm 5286 BY4741 12_5 22 B 8 YLR380W CSR1 SFH2 Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress ion transport, monocarboxylic acid metabolic process, Golgi vesicle transport, lipid transport, lipid metabolic process other endomembrane system, mitochondrion, membrane, cytoplasm 5289 BY4741 12_5 22 B 9 YLR381W CTF3 CHL3 Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 mitotic cell cycle, organelle fission, DNA replication, regulation of organelle organization, proteolysis involved in cellular protein catabolic process, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, regulation of cell cycle molecular_function nucleus, chromosome 5290 BY4741 12_5 22 B 11 YLR384C IKI3 Elongator subunit IKI3|KTI7|TOT1|ELP1 Subunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) tRNA processing, transcription from RNA polymerase II promoter, RNA modification RNA binding nucleus, cytoplasm 5293 BY4741 12_5 22 B 12 YLR385C SWC7 AWS1 Protein of unknown function; component of the Swr1p complex that incorporates Htz1p into chromatin chromatin organization molecular_function nucleus, chromosome 5294 BY4741 12_5 22 C 1 YLR386W VAC14 - Enzyme regulator; involved in synthesis of phosphatidylinositol 3,5-bisphosphate, in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; binds negative (Fig4p) and positive (Fab1p) regulators of PtdIns(3,5)P(2) to control endolysosome function; similar to mammalian Vac14p lipid metabolic process protein binding, bridging membrane, vacuole, cytoplasm 5295 BY4741 12_5 22 C 2 YLR387C REH1 - Cytoplasmic 60S subunit biogenesis factor; associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains ribosomal large subunit biogenesis molecular_function ribosome, cytoplasm 5296 BY4741 12_5 22 C 3 YLR388W RPS29A ribosomal 40S subunit protein S29A|S14|YS29|S36A|S29A|YS29A Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 5297 BY4741 12_5 22 C 4 YLR390W ECM19 - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 5299 BY4741 12_5 22 C 5 YLR392C ART10 - Protein of unknown function that contains 2 PY motifs; ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene biological_process molecular_function cytoplasm 5301 BY4741 12_5 22 C 6 YLR393W ATP10 - Assembly factor for the F0 sector of mitochondrial F1F0 ATP synthase; mitochondrial inner membrane protein; interacts genetically with ATP6 mitochondrion organization, protein complex biogenesis unfolded protein binding membrane, mitochondrial envelope, mitochondrion, cytoplasm 5302 BY4741 12_5 22 C 7 YLR395C COX8 cytochrome c oxidase subunit VIII Subunit VIII of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 5304 BY4741 12_5 22 C 8 YLR398C SKI2 SKI complex RNA helicase subunit SKI2 Ski complex component and putative RNA helicase; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, SKIV2L, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome RNA catabolic process RNA binding, mRNA binding, helicase activity, hydrolase activity cytoplasm 5307 BY4741 12_5 22 C 9 YLR400W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 5309 BY4741 12_5 22 C 10 YLR401C DUS3 tRNA dihydrouridine synthase DUS3 Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress tRNA processing, RNA modification RNA binding, mRNA binding, oxidoreductase activity nucleus, cytoplasm 5310 BY4741 12_5 22 C 11 YLR402W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 5311 BY4741 12_5 22 C 12 YLR404W SEI1 seipin|FLD1 Seipin involved in lipid droplet (LD) assembly; controls lipid particle morphology, number, and size; promotes initiation of LD formation on the ER, and ensures that LDs bud from the ER towards the cytosolic side of the membrane; forms a complex with Ldb1p at ER-LD contact sites, and stabilizes the sites of contact; integral ER membrane protein; human homolog BSCL2 implicated in congenital lipodystrophy, and can complement the yeast null mutant other molecular_function membrane, cell cortex, endomembrane system, endoplasmic reticulum, cytoplasm 5313 BY4741 12_5 22 D 1 YLR405W DUS4 - Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p tRNA processing, RNA modification oxidoreductase activity cellular_component 5314 BY4741 12_5 22 D 2 YLR407W - - Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus biological_process molecular_function cellular_component 5316 BY4741 12_5 22 D 3 YLR408C BLS1 BLB1 Subunit of the BLOC-1 complex involved in endosomal maturation; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene other molecular_function endomembrane system, cytoplasm 5317 BY4741 12_5 22 D 4 YLR410W VIP1 inositol polyphosphate kinase VIP1 Inositol hexakisphosphate and inositol heptakisphosphate kinase; inositol heptakisphosphate (IP7) production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1; inositol hexakisphosphate is also known as IP6 other kinase activity, transferase activity cytoplasm 5319 BY4741 12_5 22 D 5 YLR412W BER1 - Protein involved in microtubule-related processes; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene other molecular_function cytoplasm 5320 BY4741 12_5 22 D 6 YLR413W INA1 - Putative protein of unknown function; not an essential gene; YLR413W has a paralog, FAT3, that arose from the whole genome duplication biological_process molecular_function membrane, plasma membrane 5321 BY4741 12_5 22 D 7 YLR414C PUN1 - Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress cell wall organization or biogenesis, protein complex biogenesis molecular_function cellular bud, membrane, plasma membrane, cytoplasm 5322 BY4741 12_5 22 D 8 YLR415C - - Putative protein of unknown function; YLR415C is not an essential gene biological_process molecular_function cellular_component 5323 BY4741 12_5 22 D 9 YLR416C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 5324 BY4741 12_5 22 D 10 YLR417W VPS36 ESCRT-II subunit protein VPS36|VPL11|VAC3|GRD12 Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome; plays a role in the formation of mutant huntingtin (Htt) aggregates in yeast response to chemical, protein targeting, proteolysis involved in cellular protein catabolic process, transcription from RNA polymerase II promoter ubiquitin-like protein binding endomembrane system, membrane, cytoplasm 5325 BY4741 12_5 22 D 11 YLR418C CDC73 - Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress; human homologue, parafibromin, is a tumour suppressor linked to breast, renal and gastric cancers transcription from RNA polymerase I promoter, peptidyl-amino acid modification, chromatin organization, DNA recombination, mRNA processing, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation, DNA repair, protein alkylation, protein phosphorylation, regulation of organelle organization, cellular response to DNA damage stimulus, histone modification, regulation of protein modification process, protein modification by small protein conjugation or removal, regulation of DNA metabolic process chromatin binding, enzyme binding, ion binding, transcription factor activity, protein binding nucleus, chromosome 5326 BY4741 12_5 22 D 12 YLR421C RPN13 proteasome regulatory particle lid subunit RPN13 Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress proteolysis involved in cellular protein catabolic process ubiquitin-like protein binding cytoplasm 5328 BY4741 12_5 22 E 1 YLR228C ECM22 - Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes upon sterol depletion, after relocating from intracellular membranes to perinuclear foci; redundant activator of filamentation with UPC2, up-regulating the expression of genes involved in filamentous growth; contains Zn[2]-Cys[6] binuclear cluster; ECM22 has a paralog, UPC2, that arose from the whole genome duplication transmembrane transport, regulation of transport, lipid metabolic process, lipid transport, response to starvation, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, membrane, cytoplasm 5137 BY4741 12_4 22 E 2 YLR231C BNA5 kynureninase Kynureninase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p cofactor metabolic process, nucleobase-containing small molecule metabolic process hydrolase activity nucleus, cytoplasm 5140 BY4741 12_4 22 E 3 YLR232W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5 biological_process molecular_function cellular_component 5141 BY4741 12_4 22 E 4 YLR233C EST1 - TLC1 RNA-associated factor involved in telomere length regulation; recruitment subunit of telomerase; has G-quadruplex promoting activity required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA; EST1 has a paralog, EBS1, that arose from the whole genome duplication telomere organization, DNA replication RNA binding, DNA binding nucleus, nucleolus 5142 BY4741 12_4 22 E 5 YLR234W TOP3 DNA topoisomerase 3|EDR1 DNA Topoisomerase III; conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially; DNA catenation/decatenation activity is stimulated by RPA and Sgs1p-Top3p-Rmi1p; involved in telomere stability and regulation of mitotic recombination mitotic cell cycle, meiotic cell cycle, telomere organization, organelle fission, DNA replication, chromosome segregation, DNA recombination, regulation of DNA metabolic process isomerase activity nucleus, chromosome 5143 BY4741 12_4 22 E 6 YLR235C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORFs TOP3/YLR234W and YLR236C cellular response to DNA damage stimulus molecular_function cellular_component 5144 BY4741 12_4 22 E 7 YLR236C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF YLR235C biological_process molecular_function cellular_component 5145 BY4741 12_4 22 E 8 YLR238W FAR10 - Protein involved in recovery from arrest in response to pheromone; acts in a cell cycle arrest recovery pathway independent from Far1p; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate; FAR10 has a paralog, VPS64, that arose from the whole genome duplication response to chemical, conjugation, signaling molecular_function endomembrane system, endoplasmic reticulum, cytoplasm 5147 BY4741 12_4 22 E 9 YLR239C LIP2 lipoyl(octanoyl) transferase LIP2 Lipoyl ligase; involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups other ligase activity mitochondrion, cytoplasm 5148 BY4741 12_4 22 E 10 YLR241W CSC1 - Calcium permeable gated cation channel; may be involved in detoxification; similar to Arabidopsis CSC1 ion transport transmembrane transporter activity membrane, vacuole, cytoplasm 5150 BY4741 12_4 22 E 11 YLR242C ARV1 sterol homeostasis protein ARV1 Cortical ER protein; implicated in the membrane insertion of tail-anchored C-terminal single transmembrane domain proteins; may function in transport of glycosylphosphatidylinositol intermediates into ER lumen; required for normal intracellular sterol distribution; human ARV1 is required for normal cholesterol and bile acid homeostasis, can complement yeast arv1 null mutant transmembrane transport, lipid transport, lipid metabolic process, protein lipidation, endocytosis molecular_function cell cortex, endomembrane system, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm 5151 BY4741 12_4 22 E 12 YLR247C IRC20 E3 ubiquitin-protein ligase IRC20 E3 ubiquitin ligase and putative helicase; involved in synthesis-dependent strand annealing-mediated homologous recombination; ensures precise end-joining along with Srs2p in the Yku70p/Yku80p/Lig4p-dependent nonhomologous end joining (NHEJ) pathway; localizes to both the mitochondrion and the nucleus; contains a Snf2/Swi2 family ATPase/helicase and a RING finger domain; interacts with Cdc48p and Smt3p; null mutant displays increased levels of spontaneous Rad52p foci DNA repair, cellular response to DNA damage stimulus, DNA recombination transferase activity, hydrolase activity, helicase activity nucleus, mitochondrion, cytoplasm 5156 BY4741 12_4 22 F 1 YLR248W RCK2 serine/threonine protein kinase RCK2|CMK3|CLK1 Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication meiotic cell cycle, response to chemical, protein phosphorylation, organelle fission, regulation of organelle organization, response to oxidative stress, signaling, regulation of cell cycle, response to osmotic stress kinase activity, transferase activity cytoplasm 5157 BY4741 12_4 22 F 2 YLR250W SSP120 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function cytoplasm 5159 BY4741 12_4 22 F 3 YLR251W SYM1 ethanol metabolism protein Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17; human homolog MPV17 is implicated in hepatocerebral mtDNA depletion syndromes (MDDS), and complements yeast null mutant other molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 5160 BY4741 12_4 22 F 4 YLR252W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism biological_process molecular_function cellular_component 5161 BY4741 12_4 22 F 5 YLR253W MCP2 MRX13 Mitochondrial protein of unknown function involved in lipid homeostasis; associates with mitochondrial ribosome; integral membrane protein that localizes to the mitochondrial inner membrane; involved in mitochondrial morphology; non-essential gene which interacts genetically with MDM10, and other members of the ERMES complex; transcription is periodic during the metabolic cycle; homologous to human aarF domain containing kinase, ADCK1 mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 5162 BY4741 12_4 22 F 6 YLR254C NDL1 - Homolog of nuclear distribution factor NudE; NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends other cytoskeletal protein binding nucleus, cytoskeleton, cytoplasm 5163 BY4741 12_4 22 F 7 YLR255C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 5164 BY4741 12_4 22 F 8 YLR257W - - Protein of unknown function; protein abundance increases in response to DNA replication stress biological_process molecular_function cytoplasm 5166 BY4741 12_4 22 F 9 YLR258W GSY2 glycogen (starch) synthase GSY2 Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress carbohydrate metabolic process, generation of precursor metabolites and energy transferase activity, transferring glycosyl groups, transferase activity nucleus, cytoplasm 5167 BY4741 12_4 22 F 10 YLR260W LCB5 sphinganine kinase LCB5 Minor sphingoid long-chain base kinase; possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules; LCB5 has a paralog, LCB4, that arose from the whole genome duplication response to heat, lipid metabolic process, signaling kinase activity, transferase activity endomembrane system, membrane, Golgi apparatus, cytoplasm 5169 BY4741 12_4 22 F 11 YLR261C VPS63 - Putative protein of unknown function; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect; decreased levels of protein in enolase deficient mutant protein targeting molecular_function cellular_component 5170 BY4741 12_4 22 F 12 YLR262C YPT6 Rab family GTPase YPT6 Rab family GTPase; Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; resides temporarily at the Golgi, dissociates into cytosol upon arrival of the Rab GTPaseYpt32p, which also functions in the late Golgi; Golgi-localized form is bound to GTP, while cytosolic form is GDP-bound; homolog of the mammalian Rab6 endosomal transport GTPase activity, hydrolase activity endomembrane system, cytoplasm, Golgi apparatus 5171 BY4741 12_4 22 G 1 YLR263W RED1 - Protein component of the synaptonemal complex axial elements; involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; promotes interhomolog recombination by phosphorylating Hop1p; also interacts with Mec3p and Ddc1p meiotic cell cycle, organelle fission, regulation of organelle organization, chromosome segregation, regulation of cell cycle chromatin binding, DNA binding, structural molecule activity nucleus, chromosome 5172 BY4741 12_4 22 G 2 YLR264W RPS28B ribosomal 40S subunit protein S28B|S28e|YS27|S33B|S28B|RPS33B Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; has an extraribosomal function in autoregulation, in which Rps28Bp binds to a decapping complex via Edc3p, which then binds to RPS28B mRNA leading to its decapping and degradation; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication cytoplasmic translation, nucleobase-containing compound transport, RNA catabolic process, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 5173 BY4741 12_4 22 G 3 YLR265C NEJ1 LIF2 Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p DNA repair, cellular response to DNA damage stimulus DNA binding nucleus 5174 BY4741 12_4 22 G 4 YLR266C PDR8 - Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 5175 BY4741 12_4 22 G 5 YLR267W BOP2 - Protein of unknown function biological_process molecular_function cellular_component 5176 BY4741 12_4 22 G 6 YLR268W SEC22 SNAP receptor SEC22|TS26|TSL26|SLY2 R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog organelle fusion, vesicle organization, Golgi vesicle transport, membrane fusion other endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm 5177 BY4741 12_4 22 G 7 YLR269C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data meiotic cell cycle, sporulation, transcription from RNA polymerase II promoter molecular_function cellular_component 5178 BY4741 12_4 22 G 8 YLR270W DCS1 DcpS Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; acts as inhibitor of neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress; DCS1 has a paralog, DCS2, that arose from the whole genome duplication RNA catabolic process hydrolase activity, enzyme regulator activity nucleus, mitochondrion, cytoplasm 5179 BY4741 12_4 22 G 9 YLR271W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus, cytoplasm 5180 BY4741 12_4 22 G 10 YLR273C PIG1 protein phosphatase regulator PIG1 Putative targeting subunit for type-1 protein phosphatase Glc7p; tethers Glc7p to Gsy2p glycogen synthase; PIG1 has a paralog, GAC1, that arose from the whole genome duplication carbohydrate metabolic process, generation of precursor metabolites and energy enzyme regulator activity cytoplasm 5182 BY4741 12_4 22 G 11 YLR278C - - Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene biological_process DNA binding nucleus 5187 BY4741 12_4 22 G 12 YLR279W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 5188 BY4741 12_4 22 H 1 YLR280C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 5189 BY4741 12_4 22 H 3 YLR281C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene biological_process molecular_function mitochondrion, cytoplasm 5190 BY4741 12_4 22 H 4 YLR282C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition biological_process molecular_function cellular_component 5191 BY4741 12_4 22 H 5 YLR283W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene biological_process molecular_function mitochondrion, cytoplasm 5192 BY4741 12_4 22 H 6 YLR284C ECI1 dodecenoyl-CoA isomerase Peroxisomal delta3,delta2-enoyl-CoA isomerase; hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced; ECI1 has a paralog, DCI1, that arose from the whole genome duplication lipid metabolic process, monocarboxylic acid metabolic process isomerase activity peroxisome, cytoplasm 5193 BY4741 12_4 22 H 7 YLR285W NNT1 EFM7|S-adenosylmethionine-dependent methyltransferase S-adenosylmethionine-dependent methyltransferase; novel N-terminal protein methyltransferase that trimethylates the N-terminal glycine residue (G2) and also dimethylates lysine (K3) on elongation factor eEF1A (Tef1p/Tef2p); has a role in rDNA silencing and in lifespan determination peptidyl-amino acid modification, protein alkylation, chromatin organization transferase activity, methyltransferase activity cytoplasm 5194 BY4741 12_4 22 H 8 YLR287C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene biological_process molecular_function cytoplasm 5196 BY4741 12_4 22 H 9 YLR287-A cytoplasm cytoplasm cytoplasm - - - 5197 BY4741 12_4 22 H 10 YLR288C MEC3 PSO9|PIP3 DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 telomere organization, chromatin organization, DNA recombination, cellular response to DNA damage stimulus DNA binding nucleus, chromosome 5198 BY4741 12_4 22 H 11 YLR289W GUF1 GTPase GUF1 Mitochondrial matrix GTPase; associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor regulation of translation GTPase activity, hydrolase activity ribosome, mitochondrion, cytoplasm 5199 BY4741 12_4 22 H 12 YLR290C COQ11 ubiquinone biosynthesis protein COQ11|MRX2 Putative oxidoreductase, subunit of Coenzyme Q biosynthetic complexes; required for synthesis of wild-type levels of Coenzyme Q (ubiquinone); member of the short-chain dehydrogenase/reductase (SDR) superfamily; orthologous gene in some other fungi is fused to the COQ10 ortholog cofactor metabolic process molecular_function mitochondrion, cytoplasm 5200 BY4741 12_4 23 A 1 YLR292C SEC72 Sec63 complex subunit SEC72|SIM2|SEC67 Non-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec63p, Sec62p, and Sec66p protein targeting, transmembrane transport protein transporter activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 5202 BY4741 12_4 23 A 2 YLR294C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 generation of precursor metabolites and energy, cellular respiration molecular_function cellular_component 5204 BY4741 12_4 23 A 3 YLR295C ATP14 F1F0 ATP synthase subunit h Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress mitochondrion organization, transmembrane transport, ion transport, protein complex biogenesis, nucleobase-containing small molecule metabolic process hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 5205 BY4741 12_4 23 A 4 YLR296W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 5206 BY4741 12_4 23 A 5 YLR297W - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication biological_process molecular_function nucleus, membrane, vacuole, cytoplasm 5207 BY4741 12_4 23 A 6 YLR299W ECM38 gamma-glutamyltransferase|CIS2 Gamma-glutamyltranspeptidase; major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation response to chemical peptidase activity, hydrolase activity vacuole, cytoplasm 5209 BY4741 12_4 23 A 7 YLR300W EXG1 SCW6|BGL1 Major exo-1,3-beta-glucanase of the cell wall; involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes; EXG1 has a paralog, SPR1, that arose from the whole genome duplication carbohydrate metabolic process, cell wall organization or biogenesis hydrolase activity, hydrolase activity, acting on glycosyl bonds cell wall, extracellular region 5210 BY4741 12_4 23 A 8 YLR303W MET17 bifunctional cysteine synthase/O-acetylhomoserine aminocarboxypropyltransferase MET17|MET25|MET15 O-acetyl homoserine-O-acetyl serine sulfhydrylase; required for Methionine and cysteine biosynthesis cellular amino acid metabolic process lyase activity, transferase activity membrane, plasma membrane, cytoplasm 5211 BY4741 12_4 23 A 9 YLR304C ACO1 aconitate hydratase ACO1|GLU1 Aconitase; required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy; human homolog ACO2 can complement yeast null mutant mitochondrion organization, generation of precursor metabolites and energy, cellular respiration lyase activity, DNA binding mitochondrion, cytoplasm 5212 BY4741 12_4 23 A 10 YLR306W UBC12 NEDD8-conjugating protein UBC12 Enzyme that mediates the conjugation of Rub1p; a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes protein modification by small protein conjugation or removal transferase activity cellular_component 5214 BY4741 12_4 23 A 11 YLR307W CDA1 chitin deacetylase CDA1 Chitin deacetylase; together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall meiotic cell cycle, sporulation, cell wall organization or biogenesis hydrolase activity cell wall, membrane 5215 BY4741 12_4 23 A 12 YLR308W CDA2 chitin deacetylase CDA2 Chitin deacetylase; together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall meiotic cell cycle, sporulation, cell wall organization or biogenesis hydrolase activity cell wall 5216 BY4741 12_4 "mates like alpha, no growth on -met, growth on -lys. Confirmed Alpha -- CORRECT STRAIN CAN BE FOUND IN PLATE 122 D4" 23 B 1 YLR309C IMH1 SYS3 Protein involved in vesicular transport; mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi Golgi vesicle transport molecular_function endomembrane system, cytoplasm, Golgi apparatus 5217 BY4741 12_4 23 B 2 YLR311C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane 5219 BY4741 12_4 23 B 3 YLR312C ATG39 QNQ1 Autophagy receptor with a role in degradation of the ER and nucleus; involved specifically in autophagy of perinuclear endoplasmic reticulum in response to nitrogen starvation or rapamycin treatment; localizes to the perinuclear ER other protein binding, bridging endomembrane system, endoplasmic reticulum, cytoplasm 5220 BY4741 12_4 23 B 4 YLR312W-A MRPL15 mitochondrial 54S ribosomal protein YmL15|YmL15 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 5221 BY4741 12_4 "slow growth, petite" 23 B 5 YLR313C SPH1 YLR312C-B Protein involved in shmoo formation and bipolar bud site selection; localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p; SPH1 has a paralog, SPA2, that arose from the whole genome duplication mitotic cell cycle, response to chemical, cell morphogenesis, cell budding, cytokinesis, conjugation, pseudohyphal growth molecular_function cellular bud, site of polarized growth 5222 BY4741 12_4 23 B 6 YLR315W NKP2 - Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 chromosome segregation molecular_function cytoskeleton, microtubule organizing center, chromosome 5224 BY4741 12_4 23 B 7 YLR318W EST2 TERT Reverse transcriptase subunit of the telomerase holoenzyme; essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia telomere organization, DNA replication transferase activity, DNA binding, nucleotidyltransferase activity nucleus, nucleolus 5227 BY4741 12_4 slow growth 23 B 8 YLR319C BUD6 AIP3 Actin- and formin-interacting protein; participates in actin cable assembly and organization as a nucleation-promoting factor (NPF) for formins Bni1p and Bnr1p; a triple helical coiled-coil domain in the C-terminal region interacts with Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate mitotic cell cycle, chromatin organization, cytoskeleton organization, cell budding, cytokinesis, regulation of organelle organization, pseudohyphal growth cytoskeletal protein binding, enzyme regulator activity cell cortex, cytoskeleton, microtubule organizing center, cellular bud, membrane, site of polarized growth, cytoplasm 5228 BY4741 12_4 23 B 9 YLR320W MMS22 SLM2 Subunit of E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation meiotic cell cycle, DNA repair, organelle fission, DNA replication, cellular response to DNA damage stimulus, chromosome segregation, DNA recombination molecular_function nucleus 5229 BY4741 12_4 23 B 10 YLR322W VPS65 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth protein targeting molecular_function cellular_component 5231 BY4741 12_4 23 B 12 YDR147W EKI1 bifunctional choline kinase/ethanolamine kinase EKI1 Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication lipid metabolic process kinase activity, transferase activity cytoplasm 3505 BY4741 04_5 23 C 1 YDR148C KGD2 alpha-ketoglutarate dehydrogenase KGD2 Dihydrolipoyl transsuccinylase; component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated mitochondrion organization, generation of precursor metabolites and energy, cellular respiration transferase activity mitochondrion, cytoplasm 3506 BY4741 04_5 "slow growth, petite" 23 C 2 YDR149C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1/YDR150W; null mutation blocks anaerobic growth biological_process molecular_function cellular_component 3507 BY4741 04_5 23 C 3 YDR150W NUM1 PAC12 Protein required for nuclear migration; component of the mitochondria-ER-cortex-ancor (MECA); required for the association of mitochondria with the cell cortex and for accurate distribution of mitochondrial network; interacts with Mdm36p to link the ER and mitochondria at the cortex; localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex organelle fission, mitochondrion organization, cytoskeleton organization, organelle inheritance cytoskeletal protein binding cell cortex, endomembrane system, mitochondrion, cellular bud, site of polarized growth, endoplasmic reticulum, cytoplasm 3508 BY4741 04_5 23 C 4 YDR151C CTH1 putative mRNA-binding protein CTH1 Member of the CCCH zinc finger family; similar to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis; CTH1 has a paralog, TIS11, that arose from the whole genome duplication RNA catabolic process, cellular ion homeostasis RNA binding, mRNA binding nucleus 3509 BY4741 04_5 23 C 5 YDR152W GIR2 - Highly-acidic RWD domain-containing cytoplasmic protein; forms a highly conserved complex with Rbg2p that is responsible for efficient cell growth under amino acid starvation and binds translational activator Gcn1p in dose-dependent manner according to stress level; associates with translating ribosomes; intrinsically unstructured protein whose stability is enhanced upon binding Rbg2p cytoplasmic translation molecular_function cytoplasm 3510 BY4741 04_5 23 C 6 YDR153C ENT5 - Protein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Golgi vesicle transport, endosomal transport ion binding, lipid binding membrane, cytoplasmic membrane-bounded vesicle, endomembrane system, cytoplasm 3511 BY4741 04_5 23 C 7 YDR154C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component 3512 BY4741 04_5 23 C 8 YDR155C CPR1 peptidylprolyl isomerase CPR1|CPH1|CYP1 Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; N-terminally propionylated in vivo; protein abundance increases in response to DNA replication stress meiotic cell cycle, organelle fission, chromatin organization, sporulation, regulation of organelle organization, histone modification, regulation of cell cycle RNA binding, isomerase activity, mRNA binding nucleus, chromosome, mitochondrion, mitochondrial envelope, cytoplasm 3513 BY4741 04_5 23 C 9 YDR156W RPA14 DNA-directed RNA polymerase I subunit RPA14|A14 RNA polymerase I subunit A14 transcription from RNA polymerase I promoter transferase activity, nucleotidyltransferase activity nucleus, nucleolus 3514 BY4741 04_5 23 C 10 YDR157W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 3515 BY4741 04_5 23 C 11 YDR158W HOM2 aspartate-semialdehyde dehydrogenase|THR2 Aspartic beta semi-aldehyde dehydrogenase; catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis cellular amino acid metabolic process oxidoreductase activity nucleus, membrane, plasma membrane, cytoplasm 3516 BY4741 04_5 no growth on drop-in media 23 C 12 YDR159W SAC3 LEP1 mRNA export factor; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) mitotic cell cycle, DNA repair, organelle fission, cellular response to DNA damage stimulus, nucleobase-containing compound transport, ribosomal small subunit biogenesis, RNA catabolic process, mRNA processing, nuclear transport other nucleus, endomembrane system 3517 BY4741 04_5 slow growth 23 D 1 YDR161W ACL4 - Specific assembly chaperone for ribosomal protein Rpl4p; binds to an evolutionarily conserved surface extension of nascent Rpl4p and chaperones Rpl4p until its assembly into the pre-ribosome; transcriptionally co-regulated with rRNA and ribosome biosynthesis genes response to chemical, proteolysis involved in cellular protein catabolic process molecular_function nucleus, cytoplasm 3519 BY4741 04_5 23 D 2 YDR162C NBP2 - Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) response to heat, protein phosphorylation, regulation of protein modification process, cell wall organization or biogenesis, signaling, response to osmotic stress protein binding, bridging nucleus, cytoplasm 3520 BY4741 04_5 23 D 3 YDR163W CWC15 U2-type spliceosomal complex subunit CWC15 Non-essential protein involved in pre-mRNA splicing; component of a complex containing Cef1p; has similarity to S. pombe Cwf15p RNA splicing, mRNA processing molecular_function nucleus 3521 BY4741 04_5 23 D 4 YDR165W TRM82 - Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p; relocalizes to the cytosol in response to hypoxia; mutation in human ortholog WDR4 causes microcephalic primordial dwarfism tRNA processing, RNA modification transferase activity, methyltransferase activity nucleus, cytoplasm 3523 BY4741 04_5 23 D 5 YDR169C STB3 - Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress response to chemical, transcription from RNA polymerase II promoter DNA binding nucleus, cytoplasm 3527 BY4741 04_5 23 D 6 YDR171W HSP42 heat shock protein HSP42 Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress cytoskeleton organization unfolded protein binding cytoskeleton, cytoplasm 3529 BY4741 04_5 23 D 7 YDR173C ARG82 inositol polyphosphate multikinase|ARGRIII|IPK2|ARGR3 Inositol polyphosphate multikinase (IPMK); sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes lipid metabolic process, transcription from RNA polymerase II promoter, cellular amino acid metabolic process protein binding, bridging, kinase activity, transferase activity nucleus 3531 BY4741 04_5 23 D 8 YDR175C RSM24 mitochondrial 37S ribosomal protein RSM24 Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 3533 BY4741 04_5 "slow growth, petite" 23 D 9 YDR176W NGG1 histone acetyltransferase NGG1|SWI7|ADA3 Subunit of chromatin modifying histone acetyltransferase complexes; member of the ADA complex, the SAGA complex, and the SLIK complex; transcriptional regulator involved in glucose repression of Gal4p-regulated genes peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity nucleus 3534 BY4741 04_5 23 D 10 YDR178W SDH4 succinate dehydrogenase membrane anchor subunit SDH4|ACN18 Membrane anchor subunit of succinate dehydrogenase (SDH); involved in coupling the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; has similarity to human SDH subunit D (SDHD), which is implicated in paraganglioma generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 3536 BY4741 04_5 23 D 11 YDR179C CSN9 - Subunit of the Cop9 signalosome; Cop9 signalosome is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling response to chemical, protein modification by small protein conjugation or removal, conjugation, signaling molecular_function nucleus 3537 BY4741 04_5 23 D 12 YDR179W-A NVJ3 - Protein with a potential role in tethering ER and vacuoles; localizes to nucleus-vacuole junctions in an Mdm1p-dependent manner; contains a lipid-binding PXA domain other ion binding, lipid binding other 3538 BY4741 04_5 23 E 1 YDR181C SAS4 - Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p chromatin organization transferase activity nucleus, chromosome 3540 BY4741 04_5 23 E 2 YDR183W PLP1 - Protein that interacts with CCT (chaperonin containing TCP-1) complex; has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators response to chemical, protein folding, transcription from RNA polymerase II promoter other cellular_component 3542 BY4741 04_5 23 E 3 YDR184C ATC1 LIC4 Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress mitotic cell cycle, cellular ion homeostasis, cell budding, cytokinesis molecular_function nucleus, cytoplasm 3543 BY4741 04_5 23 E 4 YDR185C UPS3 GEP2 Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations; UPS3 has a paralog, UPS2, that arose from the whole genome duplication mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 3544 BY4741 04_5 23 E 5 YDR186C - - Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function ribosome, cytoplasm 3545 BY4741 04_5 23 E 6 YDR191W HST4 NAD-dependent histone deacetylase HST4 NAD(+)-dependent protein deacetylase; deacetylation targets are primarily mitochondrial proteins; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism; accumulates in mitochondria in response to biotin starvation and may link biotin metabolism with energy homeostasis; member of the Sir2 family and may be the functional equivalent of human SIRT3 chromatin organization, monocarboxylic acid metabolic process, histone modification, lipid metabolic process, response to starvation hydrolase activity nucleus, mitochondrion, cytoplasm 3550 BY4741 04_5 23 E 7 YDR192C NUP42 FG-nucleoporin NUP42|UIP1|RIP1 FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p response to heat, protein targeting, nucleobase-containing compound transport, nuclear transport, response to osmotic stress structural molecule activity nucleus, membrane, endomembrane system 3551 BY4741 04_5 23 E 8 YDR193W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane 3552 BY4741 04_5 23 E 9 YDR194C MSS116 - Mitochondrial transcription elongation factor; DEAD-box protein; required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing; promotes RNA folding by stabilizing an early assembly intermediate RNA splicing, mitochondrion organization, DNA-templated transcription, elongation RNA binding, hydrolase activity, ATPase activity, helicase activity, mRNA binding mitochondrion, cytoplasm 3553 BY4741 04_5 "slow growth, petite" 23 E 10 YDR195W REF2 RNA-processing protein REF2 RNA-binding protein; involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes; relocalizes to the cytosol in response to hypoxia snoRNA processing, mRNA processing, transcription from RNA polymerase II promoter, DNA-templated transcription, termination chromatin binding, RNA binding nucleus, cytoplasm 3554 BY4741 04_5 slow growth 23 E 11 YDR197W CBS2 CBP7 Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other ribosome, mitochondrion, membrane, mitochondrial envelope, cytoplasm 3556 BY4741 04_5 slow growth 23 E 12 YDR198C RKM2 - Ribosomal protein lysine methyltransferase; responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp peptidyl-amino acid modification, protein alkylation transferase activity, methyltransferase activity cellular_component 3557 BY4741 04_5 23 F 1 YDR199W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64/YDR200C; computationally predicted to have thiol-disulfide oxidoreductase activity biological_process molecular_function cellular_component 3558 BY4741 04_5 23 F 2 YDR200C VPS64 FAR9 Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance; VPS64 has a paralog, FAR10, that arose from the whole genome duplication response to chemical, protein targeting, conjugation, signaling molecular_function endomembrane system, membrane, endoplasmic reticulum, cytoplasm 3559 BY4741 04_5 23 F 3 YDR203W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF RAV2/YDR202C biological_process molecular_function cellular_component 3562 BY4741 04_5 23 F 4 YDR204W COQ4 ubiquinone biosynthesis protein COQ4 Protein with a role in ubiquinone (Coenzyme Q) biosynthesis; possibly functioning in stabilization of Coq7p; located on matrix face of mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex; human homolog COQ4 can complement yeast coq4 null mutant cofactor metabolic process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 3563 BY4741 04_5 "slow growth, petite" 23 F 5 YDR206W EBS1 - Protein involved in translation inhibition and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors; EBS1 has a paralog, EST1, that arose from the whole genome duplication RNA catabolic process, regulation of translation, DNA recombination molecular_function cytoplasm 3565 BY4741 04_5 23 F 6 YDR207C UME6 DNA-binding transcriptional regulator UME6|RIM16|NIM2|CAR80 Rpd3L histone deacetylase complex subunit; key transcriptional regulator of early meiotic genes; involved in chromatin remodeling and transcriptional repression via DNA looping; binds URS1 upstream regulatory sequence, represses transcription by recruiting conserved histone deacetylase Rpd3p (through co-repressor Sin3p) and chromatin-remodeling factor Isw2p; couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms compl mitotic cell cycle, meiotic cell cycle, response to chemical, organelle fission, chromatin organization, lipid metabolic process, transcription from RNA polymerase II promoter, carbohydrate metabolic process, regulation of organelle organization, cellular amino acid metabolic process, pseudohyphal growth, regulation of cell cycle nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding, transcription factor binding nucleus, chromosome 3566 BY4741 04_5 23 F 7 YDR209C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W biological_process molecular_function cellular_component 3568 BY4741 04_5 23 F 8 YDR210W - - Predicted tail-anchored plasma membrane protein; contains a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function cellular_component 3569 BY4741 04_5 23 F 9 YDR213W UPC2 MOX4 Sterol regulatory element binding protein; induces sterol biosynthetic genes, upon sterol depletion; acts as a sterol sensor, binding ergosterol in sterol rich conditions; relocates from intracellular membranes to perinuclear foci upon sterol depletion; redundant activator of filamentation with ECM22, up-regulating the expression of filamentous growth genes; contains a Zn[2]-Cys[6] binuclear cluster; UPC2 has a paralog, ECM22, that arose from the whole genome duplication response to chemical, transmembrane transport, regulation of transport, lipid metabolic process, lipid transport, response to starvation, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, membrane, cytoplasm 3572 BY4741 04_5 23 F 10 YDR214W AHA1 - Co-chaperone that binds Hsp82p and activates its ATPase activity; plays a role in determining prion variants; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress response to heat, protein folding enzyme regulator activity cytoplasm 3573 BY4741 04_5 23 F 11 YDR215C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein biological_process molecular_function cellular_component 3574 BY4741 04_5 23 F 12 YDR216W ADR1 DNA-binding transcription factor ADR1 Carbon source-responsive zinc-finger transcription factor; required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization response to chemical, chromatin organization, regulation of organelle organization, peroxisome organization, monocarboxylic acid metabolic process, lipid metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding, transcription factor binding nucleus, cytoplasm 3575 BY4741 04_5 23 G 1 YDR217C RAD9 chromatin-binding protein RAD9 DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p mitotic cell cycle, DNA repair, cellular response to DNA damage stimulus, transcription from RNA polymerase II promoter, regulation of cell cycle histone binding, enzyme regulator activity, DNA binding nucleus, chromosome 3576 BY4741 04_5 23 G 2 YDR218C SPR28 septin SPR28 Sporulation-specific homolog of the CDC3/10/11/12 family of genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation; the yeast CDC3/10/11/12 family is a family of bud neck microfilament genes meiotic cell cycle, sporulation structural molecule activity cell cortex, cytoskeleton, cell wall, membrane, cytoplasm 3577 BY4741 04_5 23 G 3 YDR219C MFB1 - Mitochondria-associated F-box protein; involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding mitochondrion organization, proteolysis involved in cellular protein catabolic process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 3578 BY4741 04_5 23 G 4 YDR220C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component 3579 BY4741 04_5 23 G 5 YDR221W GTB1 - Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress protein glycosylation, carbohydrate metabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds endomembrane system, endoplasmic reticulum, cytoplasm 3580 BY4741 04_5 23 G 6 YDR222W - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YDR222W has a paralog, YLR225C, that arose from the whole genome duplication biological_process molecular_function cytoplasm 3581 BY4741 04_5 23 G 7 YDR223W CRF1 - Transcriptional corepressor; involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain; CRF1 has a paralog, IFH1, that arose from the whole genome duplication transcription from RNA polymerase II promoter transcription factor activity, protein binding nucleus, chromosome, cytoplasm 3582 BY4741 04_5 23 G 8 YDR225W HTA1 histone H2A|SPT11|H2A1 Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; N-terminally propionylated in vivo DNA repair, chromatin organization, cellular response to DNA damage stimulus, transcription from RNA polymerase II promoter DNA binding nucleus, chromosome 3584 BY4741 04_5 23 G 9 YDR226W ADK1 adenylate kinase ADK1|AKY2|AKY1 Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress; mutations affecting Adk1p catalytic activity deregulate expression of phosphate utilization genes PHO5 and PHO84; human homolog AK1 can complement yeast adk1 mutant DNA replication, nucleobase-containing small molecule metabolic process kinase activity, transferase activity mitochondrial envelope, mitochondrion, cytoplasm 3585 BY4741 04_5 slow growth 23 G 10 YDR227W SIR4 chromatin-silencing protein SIR4|UTH2|STE9|ASD1 SIR protein involved in assembly of silent chromatin domains; silent information regulator (SIR) along with SIR2 and SIR3; involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; potentially phosphorylated by Cdc28p; some alleles of SIR4 prolong lifespan chromatin organization, regulation of organelle organization chromatin binding, DNA binding nucleus, chromosome 3586 BY4741 04_5 "does not mate, sterile" 23 G 11 YDR229W IVY1 - Phospholipid-binding protein that interacts with both Ypt7p and Vps33p; may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase vacuole organization, organelle fusion, membrane fusion lipid binding, ion binding vacuole, membrane, cytoplasm 3588 BY4741 04_5 23 G 12 YDR230W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20/YDR231C biological_process molecular_function cellular_component 3589 BY4741 04_5 "slow growth, petite" 23 H 1 YDR231C COX20 - Mitochondrial inner membrane protein; required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase mitochondrion organization, protein maturation, generation of precursor metabolites and energy, protein complex biogenesis, cellular respiration unfolded protein binding mitochondrion, membrane, mitochondrial envelope, cytoplasm 3590 BY4741 04_5 "slow growth, petite" 23 H 3 YDR233C RTN1 - Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication protein complex biogenesis, nucleus organization molecular_function cell cortex, endomembrane system, mitochondrion, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm 3592 BY4741 04_5 23 H 4 YDR234W LYS4 homoaconitate hydratase LYS4|LYS3 Homoaconitase; catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway cellular amino acid metabolic process lyase activity mitochondrion, cytoplasm 3593 BY4741 04_5 "no growth on -lys, no growth on drop-in media" 23 H 5 YDR237W MRPL7 mitochondrial 54S ribosomal protein YmL7/YmL5|YmL7|YmL5 Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 3596 BY4741 04_5 "slow growth, petite" 23 H 6 YDR239C - - Protein of unknown function; may interact with ribosomes, based on co-purification experiments biological_process molecular_function ribosome, cytoplasm 3598 BY4741 04_5 23 H 7 YDR241W BUD26 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay mitotic cell cycle, cytokinesis molecular_function cellular_component 3600 BY4741 04_5 23 H 8 YGL194C HOS2 histone deacetylase HOS2|RTL1 Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS meiotic cell cycle, protein phosphorylation, organelle fission, chromatin organization, regulation of organelle organization, histone modification, regulation of protein modification process, signaling, regulation of cell cycle hydrolase activity nucleus, chromosome, cytoplasm 4561 BY4741 07_3 23 H 9 YGL195W GCN1 AAS103|NDR1 Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA regulation of translation, translational elongation enzyme regulator activity ribosome, mitochondrion, cytoplasm 4562 BY4741 07_3 23 H 10 YGL196W DSD1 D-serine ammonia-lyase DSD1 D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate cellular amino acid metabolic process lyase activity cellular_component 4563 BY4741 07_3 23 H 11 YGL197W MDS3 - Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain; MDS3 has a paralog, PMD1, that arose from the whole genome duplication sporulation, pseudohyphal growth, signaling molecular_function mitochondrion, cytoplasm 4564 BY4741 07_3 23 H 12 YGL198W YIP4 - Protein that interacts with Rab GTPases; localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport biological_process enzyme binding membrane, endomembrane system, cytoplasm, Golgi apparatus 4565 BY4741 07_3 24 A 1 YGL199C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W biological_process molecular_function cellular_component 4566 BY4741 07_3 24 A 2 YGL200C EMP24 BST2 Component of the p24 complex; role in misfolded protein quality control; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles vesicle organization, Golgi vesicle transport molecular_function endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, endoplasmic reticulum, cytoplasm 4567 BY4741 07_3 "Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport" super slow growth 24 A 3 YGL202W ARO8 bifunctional 2-aminoadipate transaminase/aromatic-amino-acid:2-oxoglutarate transaminase Aromatic aminotransferase I; expression is regulated by general control of amino acid biosynthesis cellular amino acid metabolic process transferase activity cytoplasm 4569 BY4741 07_3 24 A 4 YGL203C KEX1 - Cell death protease essential for hypochlorite-induced apoptosis; involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins other peptidase activity, hydrolase activity endomembrane system, cytoplasm, Golgi apparatus 4570 BY4741 07_3 24 A 5 YGL205W POX1 acyl-CoA oxidase|FOX1 Fatty-acyl coenzyme A oxidase; involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix lipid metabolic process, monocarboxylic acid metabolic process oxidoreductase activity peroxisome, cytoplasm 4571 BY4741 07_3 24 A 6 YGL208W SIP2 SPM2 One of three beta subunits of the Snf1 kinase complex; involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane; SIP2 has a paralog, GAL83, that arose from the whole genome duplication protein phosphorylation, invasive growth in response to glucose limitation, protein complex biogenesis, response to starvation, signaling kinase activity, transferase activity membrane, cytoplasm, plasma membrane 4574 BY4741 07_3 24 A 7 YGL209W MIG2 MLZ1 Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced gene repression; under low glucose conditions relocalizes to mitochondrion, where it interacts with Ups1p, antagonizes mitochondrial fission factor Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth in response to glucose depletion; activated in stochastic pulses of nuclear localization in response to low glucose response to chemical, response to starvation, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, mitochondrion, cytoplasm 4575 BY4741 07_3 24 A 8 YGL210W YPT32 Rab family GTPase YPT32 Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication Golgi vesicle transport, exocytosis, endosomal transport GTPase activity, hydrolase activity endomembrane system, mitochondrion, membrane, mitochondrial envelope, Golgi apparatus, cytoplasm 4576 BY4741 07_3 24 A 9 YGL211W NCS6 TUC1|YGL210W-A Protein required for uridine thiolation of Gln, Lys, and Glu tRNAs; required for the thiolation of uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae tRNA processing, protein modification by small protein conjugation or removal, RNA modification RNA binding mitochondrion, cytoplasm 4577 BY4741 07_3 24 A 10 YGL212W VAM7 VPL24|VPS43 Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress vacuole organization, protein targeting, organelle fusion, vesicle organization, membrane fusion, membrane invagination lipid binding, ion binding vacuole, membrane, cytoplasm 4578 BY4741 07_3 slow growth 24 A 11 YGL213C SKI8 SKI complex subunit WD repeat protein SKI8|REC103 Ski complex component and WD-repeat protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype meiotic cell cycle, organelle fission, protein complex biogenesis, RNA catabolic process, DNA recombination molecular_function nucleus, chromosome, cytoplasm 4579 BY4741 07_3 24 A 12 YGL214W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C biological_process molecular_function cellular_component 4580 BY4741 07_3 24 B 1 YGL215W CLG1 - Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 other enzyme regulator activity other 4581 BY4741 07_3 24 B 2 YGL216W KIP3 tubulin-dependent ATPase KIP3 Kinesin-related antiparallel sliding motor protein; involved in mitotic spindle positioning; sliding activity promotes bipolar spindle assembly and maintenance of genome stability; inhibits spindle elongation, destabilizing late anaphase spindle microtubules that polymerize beyond the midzone mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, organelle assembly, protein complex biogenesis, regulation of cell cycle hydrolase activity, ATPase activity nucleus, cytoskeleton, cytoplasm 4582 BY4741 07_3 24 B 3 YGL217C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W biological_process molecular_function cellular_component 4583 BY4741 07_3 24 B 4 YGL218W - SRF1 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance biological_process molecular_function cellular_component 4584 BY4741 07_3 24 B 5 YGL220W FRA2 AIM15 Protein involved in negative regulation of iron regulon transcription; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel response to chemical, transcription from RNA polymerase II promoter molecular_function nucleus, cytoplasm 4586 BY4741 07_3 "slow growth, petite" 24 B 6 YGL221C NIF3 hypothetical protein Protein of unknown function; similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 4587 BY4741 07_3 24 B 7 YGL222C EDC1 - RNA-binding protein that activates mRNA decapping directly; binds to mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; EDC1 has a paralog, EDC2, that arose from the whole genome duplication RNA catabolic process, regulation of translation RNA binding, mRNA binding nucleus, cytoplasm 4588 BY4741 07_3 24 B 8 YGL224C SDT1 SSM1 Pyrimidine nucleotidase; responsible for production of nicotinamide riboside and nicotinic acid riboside; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives; SDT1 has a paralog, PHM8, that arose from the whole genome duplication nucleobase-containing small molecule metabolic process phosphatase activity, hydrolase activity cellular_component 4590 BY4741 07_3 24 B 9 YGL226C-A OST5 - Zeta subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins protein glycosylation, carbohydrate metabolic process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 4592 BY4741 07_3 24 B 10 YGL226W MTC3 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 biological_process molecular_function mitochondrion, cytoplasm 4593 BY4741 07_3 24 B 11 YGL227W VID30 glucose-induced degradation complex subunit VID30|GID1 Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process molecular_function nucleus, cytoplasm 4594 BY4741 07_3 24 C 1 YGL228W SHE10 - Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; putative GPI-anchored protein; overexpression causes growth arrest; SHE10 has a paralog, OSW7/YFR039C, that arose from the whole genome duplication and deletion of both SHE10 and OWS7 results in reduced dityrosine fluorescence from the spore wall relative to other mutants meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function cellular_component 4595 BY4741 07_3 24 C 2 YGL229C SAP4 - Protein required for function of the Sit4p protein phosphatase; member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p; SAP4 has a paralog, SAP155, that arose from the whole genome duplication mitotic cell cycle phosphatase activity, hydrolase activity mitochondrion, cytoplasm 4596 BY4741 07_3 24 C 3 YGL230C - - Putative protein of unknown function; non-essential gene biological_process molecular_function mitochondrion, cytoplasm 4597 BY4741 07_3 24 C 4 YGL231C EMC4 chaperone EMC4 Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4 and fly CG11137; mutation is functionally complemented by human EMC4 protein folding molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 4598 BY4741 07_3 24 C 5 YGL232W TAN1 putative tRNA acetyltransferase Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress tRNA processing, RNA modification RNA binding nucleus, cytoplasm 4599 BY4741 07_3 24 C 6 YGL234W ADE5,7 bifunctional aminoimidazole ribotide synthase/glycinamide ribotide synthase Enzyme of the 'de novo' purine nucleotide biosynthetic pathway; contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities nucleobase-containing small molecule metabolic process ligase activity cytoplasm 4601 BY4741 07_3 "slow, no growth on drop-in media" 24 C 7 YGL235W - - Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 biological_process molecular_function cellular_component 4602 BY4741 07_3 24 C 8 YGL236C MTO1 tRNA modification protein MTO1|IPS1 Mitochondrial protein; forms heterodimer complex with Mss1p that performs 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants; human homolog MTO1 can complement yeast null mutant tRNA processing, mitochondrion organization, RNA modification molecular_function mitochondrion, cytoplasm 4603 BY4741 07_3 24 C 9 YGL237C HAP2 - Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding; respiratory defect of the null mutant is functionally complemented by human NFYA response to chemical, transcription from RNA polymerase II promoter, generation of precursor metabolites and energy, cellular respiration nucleic acid binding transcription factor activity, DNA binding nucleus 4604 BY4741 07_3 24 C 10 YGL241W KAP114 - Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress protein targeting, nuclear transport protein transporter activity nucleus, membrane, cytoplasm 4608 BY4741 07_3 "slow growth, petite" 24 C 11 YGL242C - - Protein of unknown function; N-terminally propionylated in vivo; deletion mutant is viable biological_process molecular_function cellular_component 4609 BY4741 07_3 24 C 12 YGL243W TAD1 tRNA-specific adenosine deaminase tRNA-specific adenosine deaminase; deaminates adenosine-37 to inosine in tRNA-Ala tRNA processing, RNA modification hydrolase activity nucleus 4610 BY4741 07_3 24 D 1 YGL244W RTF1 CSL3 Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay transcription from RNA polymerase I promoter, peptidyl-amino acid modification, snoRNA processing, chromatin organization, DNA-templated transcription, termination, mRNA processing, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation, DNA repair, protein alkylation, protein phosphorylation, regulation of organelle organization, cellular response to DNA damage stimulus, histone modification, regulation of protein modification process, protein modification by small protein conjugation or removal, regulation of DNA metabolic process RNA binding, ion binding, transcription factor activity, protein binding nucleus, chromosome 4611 BY4741 07_3 24 D 2 YGL246C RAI1 decapping nuclease Nuclear protein with decapping endonuclease activity; targets mRNAs with unmethylated 7-methylguanosine cap structures and 5'-triphosphates; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; relocalizes to the cytosol in response to hypoxia; homologous to human DOM3Z rRNA processing, ribosomal large subunit biogenesis, DNA-templated transcription, termination, RNA catabolic process, transcription from RNA polymerase II promoter hydrolase activity, enzyme regulator activity, nuclease activity nucleus, cytoplasm 4613 BY4741 07_3 "slow growth, petite" 24 D 3 YGL248W PDE1 3',5'-cyclic-nucleotide phosphodiesterase PDE1 Low-affinity cyclic AMP phosphodiesterase; controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation signaling hydrolase activity cellular_component 4615 BY4741 07_3 24 D 4 YGL249W ZIP2 - Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress peptidyl-amino acid modification, meiotic cell cycle, organelle fission, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, DNA recombination molecular_function nucleus, chromosome, mitochondrion, cytoplasm 4616 BY4741 07_3 24 D 5 YGL250W RMR1 - Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus meiotic cell cycle, organelle fission, DNA recombination molecular_function nucleus, cytoplasm 4617 BY4741 07_3 24 D 6 YGL251C HFM1 MER3 Meiosis specific DNA helicase; involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity meiotic cell cycle, organelle fission, DNA replication, chromosome segregation, DNA recombination hydrolase activity, helicase activity nucleus 4618 BY4741 07_3 24 D 7 YGL252C RTG2 - Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p response to chemical, protein targeting, chromatin organization, regulation of organelle organization, transcription from RNA polymerase II promoter, nuclear transport, signaling transcription factor activity, protein binding nucleus, chromosome, cytoplasm 4619 BY4741 07_3 24 D 8 YGL253W HXK2 hexokinase 2|SCI2|HKB|HEX1 Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; antiapoptotic; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication carbohydrate metabolic process, cofactor metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, carbohydrate transport, nucleobase-containing small molecule metabolic process kinase activity, transferase activity nucleus, mitochondrion, cytoplasm 4620 BY4741 07_3 24 D 9 YGL254W FZF1 SUL1|RSU1|NRC299 Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress ion transport, regulation of transport, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus 4621 BY4741 07_3 24 D 10 YGL255W ZRT1 high-affinity Zn(2+) transporter ZRT1 High-affinity zinc transporter of the plasma membrane; responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor transmembrane transport, ion transport transmembrane transporter activity membrane, plasma membrane 4622 BY4741 07_3 24 D 11 YGL256W ADH4 alcohol dehydrogenase ADH4|ZRG5|NRC465 Alcohol dehydrogenase isoenzyme type IV; dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency generation of precursor metabolites and energy, cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 4623 BY4741 07_3 24 D 12 YGL257C MNT2 alpha-1,3-mannosyltransferase MNT2 Mannosyltransferase; involved in adding the 4th and 5th mannose residues of O-linked glycans protein glycosylation, carbohydrate metabolic process transferase activity, transferring glycosyl groups, transferase activity endomembrane system, cytoplasm, Golgi apparatus 4624 BY4741 07_3 24 E 1 YGL258W VEL1 - Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has a paralog, YOR387C, that arose from a single-locus duplication biological_process molecular_function cytoplasm 4625 BY4741 07_3 24 E 2 YGL259W YPS5 - Protein with similarity to GPI-anchored aspartic proteases; such proteases are Yap1p and Yap3p biological_process peptidase activity, hydrolase activity cellular_component 4626 BY4741 07_3 24 E 3 YGL260W - - Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium biological_process molecular_function cellular_component 4627 BY4741 07_3 24 E 4 YGL261C PAU11 seripauperin PAU11 Putative protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 biological_process molecular_function cellular_component 4628 BY4741 07_3 24 E 5 YGL262W - - Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene biological_process molecular_function cellular_component 4629 BY4741 07_3 24 E 6 YGL263W COS12 - Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins protein targeting, endosomal transport, proteolysis involved in cellular protein catabolic process molecular_function membrane, endomembrane system, cytoplasm 4630 BY4741 07_3 24 E 7 YGR001C EFM5 protein-lysine N-methyltransferase|AML1 S-adenosylmethionine-dependent lysine methyltransferase; involved in the trimethylation of eEF1A (Tef1p/Tef2p) at lysine 79; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in budding yeast; expresses a circular RNA; originally misclassified as a N-6-adenine specific DNA methyltransferase based on sequence similarity; both Efm5p and human ortholog N6AMT2 can methylate eEF1a from either species in vitro peptidyl-amino acid modification, protein alkylation transferase activity, methyltransferase activity cytoplasm 4631 BY4741 07_3 no growth on drop-in media 24 E 8 YGR003W CUL3 cullin CUL3|CULLIN B|CULB Ubiquitin-protein ligase; forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process transferase activity nucleus, cytoplasm 4633 BY4741 07_3 24 E 9 YGR004W PEX31 - Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p; PEX31 has a paralog, PEX30, that arose from the whole genome duplication peroxisome organization molecular_function peroxisome, membrane, cytoplasm 4634 BY4741 07_3 24 E 10 YGR006W PRP18 mRNA splicing protein PRP18 Splicing factor and component of snRNP U5; factor involved in the positioning of the 3' splice site during the second catalytic step of splicing; interacts with Slu7p RNA catabolic process, RNA splicing, mRNA processing other nucleus 4636 BY4741 07_3 slow growth 24 E 11 YGR007W ECT1 ethanolamine-phosphate cytidylyltransferase|MUQ1 Ethanolamine-phosphate cytidylyltransferase; catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases; inability of the null mutant to synthesize phosphatidylethanolamine and phosphatidylcholine from ethanolamine is functionally complemented by human PCYT2 lipid metabolic process transferase activity, nucleotidyltransferase activity nucleus, cytoplasm 4637 BY4741 07_3 24 E 12 YGR008C STF2 - Protein involved in resistance to desiccation stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to F0 sector of mitochondrial F1F0 ATPase in vitro and may modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress; STF2 has a paralog, TMA10, that arose from the whole genome duplication response to chemical molecular_function cytoplasm 4638 BY4741 07_3 24 F 1 YGR010W NMA2 nicotinamide-nucleotide adenylyltransferase NMA2 Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); homolog of human NMNAT; NMA2 has a paralog, NMA1, that arose from the whole genome duplication cofactor metabolic process, nucleobase-containing small molecule metabolic process transferase activity, nucleotidyltransferase activity nucleus 4640 BY4741 07_3 24 F 2 YGR011W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4641 BY4741 07_3 24 F 3 YGR012W - putative cysteine synthase Putative cysteine synthase; localized to the mitochondrial outer membrane biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 4642 BY4741 07_3 24 F 4 YGR014W MSB2 - Mucin family member involved in various signaling pathways; functions as osmosensor in the Sho1p-mediated HOG pathway; functions in Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; processed into secreted and cell-associated forms by aspartyl protease Yps1p; potential Cdc28p substrate signaling, response to osmotic stress signal transducer activity membrane, extracellular region, site of polarized growth, plasma membrane 4644 BY4741 07_3 24 F 5 YGR015C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion biological_process molecular_function mitochondrion, cytoplasm 4645 BY4741 07_3 24 F 6 YGR016W - - Putative protein of unknown function biological_process molecular_function membrane 4646 BY4741 07_3 24 F 7 YGR017W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm biological_process molecular_function nucleus, cytoplasm 4647 BY4741 07_3 24 F 8 YGR018C - - Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W biological_process molecular_function cellular_component 4648 BY4741 07_3 24 F 9 YGR019W UGA1 4-aminobutyrate transaminase Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress monocarboxylic acid metabolic process, cellular amino acid metabolic process transferase activity cytoplasm 4649 BY4741 07_3 24 F 10 YGR020C VMA7 H(+)-transporting V1 sector ATPase subunit F Subunit F of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits cellular ion homeostasis ATPase activity, hydrolase activity, transmembrane transporter activity membrane, vacuole, cytoplasm 4650 BY4741 07_3 "slow growth, petite" 24 F 11 YGR021W - HAH1 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 4651 BY4741 07_3 24 F 12 YGR022C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W biological_process molecular_function cellular_component 4652 BY4741 07_3 24 G 1 YGR023W MTL1 - Putative plasma membrane sensor; involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p; MTL1 has a paralog, MID2, that arose from the whole genome duplication response to chemical, response to starvation, cell wall organization or biogenesis, response to oxidative stress molecular_function membrane, plasma membrane 4653 BY4741 07_3 24 G 2 YGR025W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 4655 BY4741 07_3 24 G 3 YGR026W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function membrane 4656 BY4741 07_3 24 G 4 YPL091W GLR1 glutathione-disulfide reductase GLR1|LPG17 Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; cytosolic Glr1p is the main determinant of the glutathione redox state of the mitochondrial intermembrane space; mitochondrial Glr1p has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress response to chemical, response to oxidative stress oxidoreductase activity nucleus, mitochondrion, cytoplasm 2737 BY4741 16_3 24 G 5 YPL090C RPS6A ribosomal 40S subunit protein S6A|S6e|rp9|YS4|S6A|S10A Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 2738 BY4741 16_3 24 G 6 YPL089C RLM1 - MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication transcription from RNA polymerase II promoter, signaling nucleic acid binding transcription factor activity, DNA binding nucleus 2739 BY4741 16_3 24 G 7 YPL088W - aldo-keto reductase superfamily protein Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance other oxidoreductase activity cellular_component 2740 BY4741 16_3 24 G 8 YPL087W YDC1 alkaline dihydroceramidase Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication lipid metabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm 2741 BY4741 16_3 24 G 9 YPL086C ELP3 Elongator subunit ELP3|KAT9|KTI8|TOT3|HPA1 Subunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin; human homolog ELP3 can partially complement yeast elp3 null mutant tRNA processing, transcription from RNA polymerase II promoter, RNA modification transferase activity nucleus, cytoplasm 2742 BY4741 16_3 24 G 10 YPL084W BRO1 NPI3|ASI6|VPS31|LPF2 Cytoplasmic class E vacuolar protein sorting (VPS) factor; coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes response to chemical, protein targeting, vesicle organization, proteolysis involved in cellular protein catabolic process, endosomal transport, protein modification by small protein conjugation or removal enzyme regulator activity endomembrane system, cytoplasm 2744 BY4741 16_3 24 G 11 YPL081W RPS9A ribosomal 40S subunit protein S9A|rp21|YS11|S9A|S4|S13 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis RNA binding, structural constituent of ribosome, rRNA binding, structural molecule activity ribosome, cytoplasm 2747 BY4741 16_3 24 G 12 YPL080C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 2748 BY4741 16_3 24 H 1 YPL079W RPL21B ribosomal 60S subunit protein L21B|L21e|L21B Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 2749 BY4741 16_3 24 H 3 YPL078C ATP4 F1F0 ATP synthase subunit 4|LPF7 Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase; ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex, which in turn determines the shape of inner membrane cristae; phosphorylated transmembrane transport, ion transport, protein complex biogenesis, nucleobase-containing small molecule metabolic process hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 2750 BY4741 16_3 slow growth 24 H 4 YPL077C - - Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication biological_process molecular_function cellular_component 2751 BY4741 16_3 24 H 5 YPL074W YTA6 putative AAA family ATPase YTA6 Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress biological_process ATPase activity, hydrolase activity cell cortex, cytoplasm 2754 BY4741 16_3 24 H 6 YPL072W UBP16 putative ubiquitin-specific protease UBP16 Deubiquitinating enzyme anchored to the outer mitochondrial membrane; probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria protein modification by small protein conjugation or removal peptidase activity, hydrolase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 2755 BY4741 16_3 24 H 7 YPL073C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments biological_process molecular_function ribosome, cytoplasm 2756 BY4741 16_3 24 H 8 YPL071C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 2757 BY4741 16_3 24 H 9 YPL070W MUK1 guanine nucleotide exchange factor MUK1 Guanine nucleotide exchange factor (GEF); involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); specifically stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF VPS9; required for localization of the CORVET complex to endosomes; contains a VPS9 domain Golgi vesicle transport, endosomal transport guanyl-nucleotide exchange factor activity cytoplasm 2758 BY4741 16_3 24 H 10 YPL069C BTS1 farnesyltranstransferase Geranylgeranyl diphosphate synthase (GGPS); increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic lipid metabolic process transferase activity mitochondrion, cytoplasm 2759 BY4741 16_3 24 H 11 YPL068C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus 2760 BY4741 16_3 24 H 12 YPL067C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene biological_process molecular_function cytoplasm 2761 BY4741 16_3 25 A 1 YPL066W RGL1 - Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source other molecular_function cellular bud, site of polarized growth, cytoplasm 2762 BY4741 16_3 25 A 2 YPL065W VPS28 ESCRT-I subunit protein VPS28|VPL13|VPT28 Component of the ESCRT-I complex; complex is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p; other members include Stp22p, Srn2p, Vps28p, and Mvb12p protein targeting, proteolysis involved in cellular protein catabolic process other membrane, endomembrane system, cytoplasm 2763 BY4741 16_3 25 A 3 YPL064C CWC27 putative peptidylprolyl isomerase CWC27 Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus 2764 BY4741 16_3 25 A 4 YPL062W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation biological_process molecular_function cellular_component 2766 BY4741 16_3 25 A 5 YPL061W ALD6 aldehyde dehydrogenase (NADP(+)) ALD6|ALD1 Cytosolic aldehyde dehydrogenase; activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress cofactor metabolic process, nucleobase-containing small molecule metabolic process, response to osmotic stress, monocarboxylic acid metabolic process oxidoreductase activity mitochondrion, cytoplasm 2767 BY4741 16_3 25 A 6 YPL060W MFM1 LPE10 Mitochondrial inner membrane magnesium transporter; involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 2768 BY4741 16_3 25 A 7 YPL058C PDR12 ATP-binding cassette multidrug transporter PDR12 Plasma membrane ATP-binding cassette (ABC) transporter; weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity other transmembrane transporter activity membrane, plasma membrane 2770 BY4741 16_3 25 A 8 YPL057C SUR1 mannosylinositol phosphorylceramide synthase catalytic subunit SUR1|LPE15|CSG1|BCL21 Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication lipid metabolic process transferase activity, transferring glycosyl groups, transferase activity membrane, vacuole, cytoplasm 2771 BY4741 16_3 25 A 9 YPL056C LCL1 - Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan biological_process molecular_function cellular_component 2772 BY4741 16_3 25 A 10 YPL055C LGE1 - Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division meiotic cell cycle, protein alkylation, chromatin organization, DNA replication, histone modification, protein modification by small protein conjugation or removal molecular_function nucleus 2773 BY4741 16_3 25 A 11 YPL054W LEE1 - Zinc-finger protein of unknown function biological_process molecular_function cellular_component 2774 BY4741 16_3 25 A 12 YPL053C KTR6 putative mannosyltransferase|MNN6 Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication protein glycosylation, carbohydrate metabolic process, cell wall organization or biogenesis transferase activity membrane 2775 BY4741 16_3 25 B 1 YPL052W OAZ1 YPL052W-A Regulator of ornithine decarboxylase Spe1p; antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p regulation of translation, translational elongation, proteolysis involved in cellular protein catabolic process enzyme regulator activity cytoplasm 2776 BY4741 16_3 25 B 2 YPL051W ARL3 Arf family GTPase ARL3 ARF-like small GTPase of the RAS superfamily; required for recruitment of Arl1p, a GTPase that regulates membrane traffic, to the Golgi apparatus; NatC-catalyzed N-terminal acetylation regulates Golgi membrane association mediated by interaction with membrane receptor, Sys1p; similar to ADP-ribosylation factor and orthologous to mammalian ARFRP1 Golgi vesicle transport GTPase activity, hydrolase activity membrane, endomembrane system, cytoplasm, Golgi apparatus 2777 BY4741 16_3 25 B 3 YPL049C DIG1 RST1 MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG1 has a paralog, DIG2, that arose from the whole genome duplication response to chemical, invasive growth in response to glucose limitation, pseudohyphal growth, transcription from RNA polymerase II promoter DNA binding, transcription factor binding nucleus 2779 BY4741 16_3 25 B 4 YPL048W CAM1 CPBP|TEF3 One of two isoforms of the gamma subunit of eEF1B; stimulates the release of GDP from eEF1A (Tef1p/Tef2p) post association with the ribosomal complex with eEF1Balpha subunit; nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids regulation of translation, translational elongation, transcription from RNA polymerase II promoter guanyl-nucleotide exchange factor activity, nucleic acid binding transcription factor activity, lipid binding, DNA binding, ion binding nucleus 2780 BY4741 16_3 25 B 5 YPL047W SGF11 SAGA histone acetyltransferase complex subunit SGF11 Integral subunit of SAGA histone acetyltransferase complex; regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation chromatin organization, protein modification by small protein conjugation or removal, transcription from RNA polymerase II promoter, histone modification enzyme regulator activity nucleus 2781 BY4741 16_3 25 B 6 YPL046C ELC1 elongin C Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair DNA repair, protein modification by small protein conjugation or removal, cellular response to DNA damage stimulus, proteolysis involved in cellular protein catabolic process transferase activity nucleus 2782 BY4741 16_3 25 B 7 YPL042C SSN3 cyclin-dependent serine/threonine protein kinase SSN3|URR1|SSX7|CDK8|RYE5|UME5|SRB10|NUT7|GIG2 Cyclin-dependent protein kinase; component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression RNA catabolic process, response to chemical, protein phosphorylation, transcription from RNA polymerase II promoter kinase activity, transferase activity nucleus 2786 BY4741 16_3 25 B 8 YPL041C MRX11 - Protein that associates with mitochondrial ribosome; involved in maintenance of telomere length biological_process molecular_function cellular_component 2787 BY4741 16_3 25 B 9 YPL040C ISM1 isoleucine--tRNA ligase ISM1 Mitochondrial isoleucyl-tRNA synthetase; null mutant is deficient in respiratory growth; human homolog IARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 2788 BY4741 16_3 25 B 10 YPL039W - - Putative protein of unknown function; YPL039W is not an essential gene biological_process molecular_function cellular_component 2789 BY4741 16_3 25 B 11 YPL038W MET31 - Zinc-finger DNA-binding transcription factor; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; involved in transcriptional regulation of the methionine biosynthetic genes; feedforward loop controlling expression of MET32 and the lack of such a loop for MET31 may account for the differential actions of Met31p and Met32p; MET31 has a paralog, MET32, that arose from the whole genome duplication transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding nucleus, cytoplasm 2790 BY4741 16_3 25 B 12 YPL037C EGD1 - Subunit beta1 of the nascent polypeptide-associated complex (NAC); involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b; EGD1 has a paralog, BTT1, that arose from the whole genome duplication protein targeting, protein folding unfolded protein binding cytoplasm 2791 BY4741 16_3 25 C 2 YPL035C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene biological_process molecular_function cellular_component 2794 BY4741 16_3 25 C 3 YPL033C SRL4 - Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p nucleobase-containing small molecule metabolic process molecular_function mitochondrion, cytoplasm 2795 BY4741 16_3 25 C 4 YPL032C SVL3 - Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress; SVL3 has a paralog, PAM1, that arose from the whole genome duplication endocytosis molecular_function cellular bud, site of polarized growth, cytoplasm 2796 BY4741 16_3 25 C 5 YPL030W TRM44 - tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene tRNA processing, RNA modification transferase activity, methyltransferase activity cytoplasm 2798 BY4741 16_3 25 C 6 YPL029W SUV3 ATP-dependent RNA helicase SUV3|LPB2 ATP-dependent RNA helicase; component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron RNA catabolic process, RNA splicing, DNA replication, mitochondrion organization helicase activity, ATPase activity, nuclease activity, hydrolase activity chromosome, mitochondrion, cytoplasm 2799 BY4741 16_3 25 C 7 YPL026C SKS1 putative serine/threonine protein kinase SKS1|SHA3 Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway; SKS1 has a paralog, VHS1, that arose from the whole genome duplication response to chemical, protein phosphorylation, carbohydrate transport, pseudohyphal growth, transcription from RNA polymerase II promoter kinase activity, transferase activity nucleus, cytoplasm 2802 BY4741 16_3 25 C 8 YPL025C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 2803 BY4741 16_3 25 C 9 YPL023C MET12 methylenetetrahydrofolate reductase (NAD(P)H) MET12 Protein with MTHFR activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of methylenetetrahydrofolate reductase (MTHFR) biological_process oxidoreductase activity cellular_component 2805 BY4741 16_3 25 C 10 YPL022W RAD1 RAD12|ssDNA endodeoxyribonuclease RAD1|LPB9 Single-stranded DNA endonuclease (with Rad10p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein meiotic cell cycle, DNA repair, organelle fission, cellular response to DNA damage stimulus, DNA recombination hydrolase activity, DNA binding, nuclease activity nucleus 2806 BY4741 16_3 25 C 11 YPL021W ECM23 SRD2 Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p pseudohyphal growth DNA binding cellular_component 2807 BY4741 16_3 25 C 12 YPL019C VTC3 PHM2 Subunit of vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC3 has a paralog, VTC2, that arose from the whole genome duplication vacuole organization, organelle fusion, membrane fusion, membrane invagination molecular_function endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm 2809 BY4741 16_3 25 D 1 YPL018W CTF19 MCM18 Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, proteolysis involved in cellular protein catabolic process, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, regulation of cell cycle molecular_function nucleus, chromosome 2810 BY4741 16_3 25 D 2 YPL015C HST2 histone deacetylase HST2 Cytoplasmic NAD(+)-dependent protein deacetylase; deacetylation targets are primarily cytoplasmic proteins; member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export chromatin organization, regulation of organelle organization, DNA recombination, regulation of DNA metabolic process hydrolase activity nucleus, cytoplasm 2813 BY4741 16_3 25 D 3 YPL014W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus biological_process molecular_function nucleus, cytoplasm 2814 BY4741 16_3 25 D 4 YPL013C MRPS16 mitochondrial 37S ribosomal protein MRPS16 Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 2815 BY4741 16_3 "slow growth, petite" 25 D 5 YPL009C RQC2 TAE2 Component of RQC, which mediates nascent chain degradation; RQC (ribosome quality control complex) is a ribosome-bound complex required for degradation of polypeptides arising from stalled translation; recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of nascent chains independently of mRNA or 40S subunits; monitors translation stress and signals this to Hsf1p proteolysis involved in cellular protein catabolic process RNA binding, mRNA binding ribosome, cytoplasm 2819 BY4741 16_3 25 D 6 YPL008W CHL1 MCM12|LPA9|CTF1 Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome mitotic cell cycle, DNA repair, organelle fission, cellular response to DNA damage stimulus, chromosome segregation hydrolase activity, helicase activity nucleus, chromosome 2820 BY4741 16_3 25 D 7 YPL006W NCR1 sphingolipid transporter Vacuolar membrane protein; transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; cells lacking Ncr1p exhibit high levels of long chain bases (LCB), similar to the accumulation of high amounts of lipids observed in patients with Neimann-Pick C, a disease caused by loss-of-function mutations in NPC1, the functional ortholog of Ncr1p lipid metabolic process other membrane, vacuole, cytoplasm 2822 BY4741 16_3 mates like alpha. Confirmed Alpha -- CORRECT STRAIN CAN BE FOUND IN PLATE 123 H11 25 D 8 YPL005W AEP3 - Peripheral mitochondrial inner membrane protein; may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; stabilizes the bicistronic AAP1-ATP6 mRNA translational initiation, mitochondrial translation, mitochondrion organization molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm 2823 BY4741 16_3 25 D 9 YPL003W ULA1 ENR2 Protein that activates Rub1p (NEDD8) before neddylation; acts together with Uba3p; may play a role in protein degradation protein modification by small protein conjugation or removal ligase activity cellular_component 2825 BY4741 16_3 25 D 10 YPL002C SNF8 ESCRT-II subunit protein SNF8|VPL14|VPS22 Component of the ESCRT-II complex; ESCRT-II is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression response to chemical, protein targeting, proteolysis involved in cellular protein catabolic process, transcription from RNA polymerase II promoter molecular_function endomembrane system, membrane, cytoplasm 2826 BY4741 16_3 25 D 11 YPL001W HAT1 histone acetyltransferase catalytic subunit HAT1|KAT1 Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex; uses the cofactor acetyl coenzyme A to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification chromatin binding, transferase activity, histone binding nucleus, cytoplasm 2827 BY4741 16_3 25 D 12 YPR001W CIT3 citrate (Si)-synthase CIT3 Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate lipid metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, cellular respiration transferase activity mitochondrion, cytoplasm 2828 BY4741 16_3 "slow growth, bi-mater" 25 E 1 YPR002W PDH1 putative 2-methylcitrate dehydratase Putative 2-methylcitrate dehydratase; mitochondrial protein that participates in respiration; induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate lipid metabolic process, monocarboxylic acid metabolic process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 2829 BY4741 16_3 25 E 2 YPR003C - - Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 2830 BY4741 16_3 25 E 3 YPR004C AIM45 - Putative ortholog of mammalian ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response; ETF-alpha is an electron transfer flavoprotein complex subunit biological_process molecular_function mitochondrion, cytoplasm 2831 BY4741 16_3 25 E 4 YPR005C HAL1 - Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p transcription from RNA polymerase II promoter, response to osmotic stress molecular_function cytoplasm 2832 BY4741 16_3 25 E 5 YPR106W ISR1 putative protein kinase ISR1 Predicted protein kinase; overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C biological_process kinase activity, transferase activity cellular_component 5522 BY4741 16_5 25 E 6 YPR109W - - Predicted membrane protein; diploid deletion strain has high budding index biological_process molecular_function cellular_component 5525 BY4741 16_5 25 E 7 YPR111W DBF20 serine/threonine-protein kinase DBF20 Ser/Thr kinase involved in late nuclear division; one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; DBF20 has a paralog, DBF2, that arose from the whole genome duplication mitotic cell cycle, protein phosphorylation, organelle fission, cytokinesis, RNA catabolic process kinase activity, transferase activity cytoskeleton, microtubule organizing center, cellular bud, site of polarized growth, cytoplasm 5527 BY4741 16_5 25 E 8 YPR114W - - Putative protein of unknown function biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 5530 BY4741 16_5 "grows on -met, grows on -lys " 25 E 9 YPR115W RGC1 GCA1 Putative regulator of the Fps1p glycerol channel; multiply phosphorylated by Hog1p under osmotic stress; contains a pleckstrin homology domain; forms homodimers and heterodimerizes with paralog Ask10p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm carbohydrate transport, regulation of transport other cytoplasm 5531 BY4741 16_5 25 E 10 YPR116W RRG8 - Putative protein of unknown function; required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport mitochondrion organization molecular_function mitochondrion, cytoplasm 5532 BY4741 16_5 "slow growth, petite" 25 E 11 YPR117W - BSH1 Putative protein of unknown function biological_process molecular_function cellular_component 5533 BY4741 16_5 25 E 12 YPR119W CLB2 B-type cyclin CLB2 B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB2 has a paralog, CLB1, that arose from the whole genome duplication mitotic cell cycle, protein phosphorylation, protein dephosphorylation, cytoskeleton organization, regulation of organelle organization, regulation of protein modification process, regulation of cell cycle enzyme regulator activity nucleus, cytoskeleton, microtubule organizing center, cellular bud, site of polarized growth, cytoplasm 5534 BY4741 16_5 25 F 1 YPR120C CLB5 B-type cyclin CLB5 B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase; CLB5 has a paralog, CLB6, that arose from the whole genome duplication mitotic cell cycle, meiotic cell cycle, protein phosphorylation, organelle fission, DNA replication, cytoskeleton organization, regulation of organelle organization, organelle assembly, protein complex biogenesis, regulation of protein modification process, regulation of DNA metabolic process, regulation of cell cycle enzyme regulator activity nucleus 5535 BY4741 16_5 25 F 2 YPR121W THI22 putative phosphomethylpyrimidine kinase Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis vitamin metabolic process molecular_function cellular_component 5536 BY4741 16_5 25 F 3 YPR122W AXL1 STE22|FUS5 Haploid specific endoprotease of a-factor mating pheromone; performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells mitotic cell cycle, cell budding, cytokinesis, protein maturation, conjugation peptidase activity, hydrolase activity cellular bud, site of polarized growth 5537 BY4741 16_5 25 F 4 YPR123C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR biological_process molecular_function membrane 5538 BY4741 16_5 petite 25 F 5 YPR124W CTR1 high-affinity Cu transporter CTR1 High-affinity copper transporter of plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress; human homolog SLC31A1 can complement a yeast ctr1 ctr3 double deletion ion transport transmembrane transporter activity nucleus, membrane, plasma membrane, cytoplasm 5539 BY4741 16_5 "slow growth, petite" 25 F 6 YPR125W YLH47 MRS7 Mitochondrial inner membrane protein; exposed to the mitochondrial matrix; associates with mitochondrial ribosomes; NOT required for respiratory growth; homolog of human Letm1, a protein implicated in Wolf-Hirschhorn syndrome mitochondrion organization, ion transport molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 5540 BY4741 16_5 25 F 7 YPR126C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 5541 BY4741 16_5 25 F 8 YPR127W - pyridoxine 4-dehydrogenase Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm 5542 BY4741 16_5 25 F 9 YPR128C ANT1 - Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation ion transport, peroxisome organization, nucleobase-containing compound transport, monocarboxylic acid metabolic process, lipid metabolic process transmembrane transporter activity peroxisome, membrane, cytoplasm 5543 BY4741 16_5 25 F 10 YPR129W SCD6 LSM13 Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress translational initiation, regulation of translation, organelle assembly RNA binding, mRNA binding cytoplasm 5544 BY4741 16_5 25 F 11 YPR130C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 5545 BY4741 16_5 25 F 12 YOL151W GRE2 methylglyoxal reductase (NADPH-dependent) GRE2 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; restores resistance to glycolaldehyde by coupling reduction of glycolaldehyde to ethylene glycol and oxidation of NADPH to NADP+; protein abundance increases in response to DNA replication stress; methylglyoxal reductase (NADPH-dependent) is also known as D-lactaldehyde dehydrogenase lipid metabolic process oxidoreductase activity nucleus, cytoplasm 7391 BY4741 00_18 25 G 1 YPR132W RPS23B ribosomal 40S subunit protein S23B|rp37|YS14|S28B|S23B|S12 Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal regulation of translation, rRNA processing, translational elongation, ribosomal small subunit biogenesis structural constituent of ribosome, structural molecule activity ribosome, cytoplasm 5547 BY4741 16_5 25 G 2 YPR134W MSS18 - Nuclear encoded protein needed for splicing of mitochondrial intron; required for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions RNA splicing molecular_function mitochondrion, cytoplasm 5549 BY4741 16_5 slow growth 25 G 3 YPR135W CTF4 chromatin-binding protein CTF4|POB1|CHL15 Chromatin-associated protein; required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion mitotic cell cycle, DNA repair, organelle fission, DNA replication, cellular response to DNA damage stimulus, chromosome segregation, DNA recombination chromatin binding, DNA binding nucleus, chromosome 5550 BY4741 16_5 25 G 4 YPR138C MEP3 ammonium permease MEP3 Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease; MEP3 has a paralog, MEP1, that arose from the whole genome duplication ion transport transmembrane transporter activity membrane, plasma membrane 5553 BY4741 16_5 25 G 5 YPR139C LOA1 lysophosphatidic acid acyltransferase LOA1|VPS66 Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA protein targeting transferase activity endomembrane system, endoplasmic reticulum, cytoplasm 5554 BY4741 16_5 25 G 6 YPR140W TAZ1 lysophosphatidylcholine acyltransferase Lyso-phosphatidylcholine acyltransferase; required for normal phospholipid content of mitochondrial membranes; major determinant of the final acyl chain composition of the mitochondrial-specific phospholipid cardiolipin; mutations in human ortholog tafazzin (TAZ) cause Barth syndrome, a rare X-linked disease characterized by skeletal and cardiomyopathy and bouts of cyclic neutropenia; a specific splice variant of human TAZ can complement yeast null mutant mitochondrion organization, generation of precursor metabolites and energy, protein complex biogenesis, cellular respiration, lipid metabolic process, nucleobase-containing small molecule metabolic process transferase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 5555 BY4741 16_5 25 G 7 YPR141C KAR3 OSR11 Minus-end-directed microtubule motor; functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate mitotic cell cycle, meiotic cell cycle, organelle fission, cytoskeleton organization, organelle fusion, conjugation, regulation of organelle organization, chromosome segregation, nucleus organization, regulation of cell cycle hydrolase activity, cytoskeletal protein binding, ATPase activity nucleus, cytoskeleton, microtubule organizing center, membrane, cytoplasm 5556 BY4741 16_5 25 G 8 YPR145W ASN1 asparagine synthase (glutamine-hydrolyzing) 1 Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication cellular amino acid metabolic process ligase activity cytoplasm 5560 BY4741 16_5 25 G 9 YPR146C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function mitochondrion, cytoplasm 5561 BY4741 16_5 25 G 10 YPR147C - - Putative protein of unknown function; may have a role in lipid metabolism, based on localization to lipid droplets; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS biological_process molecular_function cytoplasm 5562 BY4741 16_5 25 G 11 YPR148C - - Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function ribosome, cytoplasm 5563 BY4741 16_5 25 G 12 YPR149W NCE102 NCE2 Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs); NCE102 has a paralog, FHN1, that arose from the whole genome duplication protein phosphorylation, regulation of protein modification process molecular_function endomembrane system, mitochondrion, cellular bud, membrane, endoplasmic reticulum, cytoplasm, plasma membrane 5564 BY4741 16_5 25 H 1 YPR150W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C biological_process molecular_function membrane 5565 BY4741 16_5 25 H 3 YPR152C URN1 - Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase biological_process molecular_function nucleus 5567 BY4741 16_5 25 H 4 YPR153W - - Putative protein of unknown function biological_process molecular_function membrane 5568 BY4741 16_5 bi-mater 25 H 5 YPR154W PIN3 LSB2 Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with LSB1 cooperatively inhibits the nucleation of actin filaments; short-lived protein whose levels increase in response to thermal stress; induces the formation of the [PIN+] and [RNQ+] prions when overproduced; PIN3 has a paralog, LSB1, that arose from the whole genome duplication cytoskeleton organization, regulation of organelle organization, protein complex biogenesis molecular_function nucleus, cell cortex, cytoskeleton, cytoplasm 5569 BY4741 16_5 25 H 6 YPR155C NCA2 - Protein that regulates expression of Fo-F1 ATP synthase subunits; involved in the regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p generation of precursor metabolites and energy, cellular respiration molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 5570 BY4741 16_5 25 H 7 YPR156C TPO3 - Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication ion transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm 5571 BY4741 16_5 25 H 8 YPR157W TDA6 - Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele; TDA6 has a paralog, VPS62, that arose from the whole genome duplication biological_process molecular_function cellular_component 5572 BY4741 16_5 25 H 9 YPR158W CUR1 - Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication response to heat, protein folding other nucleus 5573 BY4741 16_5 25 H 10 YPR159W KRE6 beta-glucan synthesis-associated protein KRE6|CWH48 Type II integral membrane protein; required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p; KRE6 has a paralog, SKN1, that arose from the whole genome duplication carbohydrate metabolic process, cell wall organization or biogenesis hydrolase activity, hydrolase activity, acting on glycosyl bonds endomembrane system, cytoplasmic membrane-bounded vesicle, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm, plasma membrane 5574 BY4741 16_5 25 H 11 YPR160W GPH1 - Glycogen phosphorylase required for the mobilization of glycogen; non-essential; regulated by cyclic AMP-mediated phosphorylation; expression is regulated by stress-response elements and by the HOG MAP kinase pathway carbohydrate metabolic process, generation of precursor metabolites and energy transferase activity, transferring glycosyl groups, transferase activity cytoplasm 5575 BY4741 16_5 slow growth 25 H 12 YPR163C TIF3 eIF4B|STM1|RBL3 Translation initiation factor eIF-4B; contains an RNA recognition motif and binds to single-stranded RNA; has RNA annealing activity; interacts with Rps20p at the head of the 40S ribosomal subunit and alters the structure of the mRNA entry channel cytoplasmic translation, translational initiation, protein complex biogenesis RNA binding, mRNA binding ribosome, cytoplasm 5578 BY4741 16_5 26 A 1 YPR164W MMS1 SLM6|RTT108 Subunit of E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay RNA catabolic process, DNA repair, DNA replication, DNA recombination, cellular response to DNA damage stimulus, transposition molecular_function other 5579 BY4741 16_5 26 A 2 YPR166C MRP2 mitochondrial 37S ribosomal protein MRP2 Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 5581 BY4741 16_5 "slow growth, petite" 26 A 3 YPR167C MET16 phosphoadenylyl-sulfate reductase (thioredoxin) 3'-phosphoadenylsulfate reductase; reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism cellular amino acid metabolic process oxidoreductase activity cytoplasm 5582 BY4741 16_5 26 A 4 YPR170C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORFs YPR169W-A and YPR170W-B biological_process molecular_function cellular_component 5585 BY4741 16_5 26 A 5 YPR171W BSP1 - Adapter that links synaptojanins to the cortical actin cytoskeleton; the synaptojanins are Inp52p and Inp53p other protein binding, bridging cellular bud, membrane, cell cortex, site of polarized growth, cytoskeleton, cytoplasm 5586 BY4741 16_5 26 A 6 YPR172W - pyridoxal 5'-phosphate synthase Protein of unknown function; predicted to encode a pyridoxal 5'-phosphate synthase based on sequence similarity but purified protein does not possess this activity, nor does it bind flavin mononucleotide (FMN); transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication biological_process molecular_function nucleus, cytoplasm 5587 BY4741 16_5 26 A 7 YPR173C VPS4 AAA family ATPase VPS4|DID6|VPT10|VPL4|GRD13|END13|CSC1 AAA-ATPase involved in multivesicular body (MVB) protein sorting; ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism vesicle organization, protein complex biogenesis, lipid metabolic process, endosomal transport hydrolase activity, ion binding, ATPase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 5588 BY4741 16_5 26 A 8 YPR174C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication biological_process molecular_function nucleus, cytoskeleton, microtubule organizing center, endomembrane system 5589 BY4741 16_5 26 A 9 YPR179C HDA3 PLO1 Subunit of the HDA1 histone deacetylase complex; possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex contains Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; relocalizes to the cytosol in response to hypoxia; similar to Hda2p chromatin organization, histone modification, chromosome segregation, transcription from RNA polymerase II promoter chromatin binding, hydrolase activity, DNA binding nucleus, chromosome, cytoplasm 5594 BY4741 16_5 26 A 10 YPR184W GDB1 bifunctional 4-alpha-glucanotransferase/amylo-alpha-1,6-glucosidase Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress carbohydrate metabolic process, generation of precursor metabolites and energy transferase activity, transferring glycosyl groups, transferase activity, hydrolase activity, hydrolase activity, acting on glycosyl bonds mitochondrion, cytoplasm 5599 BY4741 16_5 26 A 11 YPR185W ATG13 serine/threonine protein kinase regulatory subunit ATG13|APG13 Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; contains a HORMA domain required for autophagy and for recruitment of the phosphatidylinositol 3-kinase complex subunit Atg14p to the pre-autophagosomal structure protein targeting, protein phosphorylation, mitochondrion organization, regulation of protein modification process, membrane invagination enzyme regulator activity membrane, cytoplasm 5600 BY4741 16_5 26 A 12 YPR188C MLC2 - Regulatory light chain for the type II myosin Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring cytokinesis cytoskeletal protein binding cellular bud, cell cortex, site of polarized growth, cytoskeleton, cytoplasm 5603 BY4741 16_5 26 B 1 YPR189W SKI3 SKI complex subunit tetratricopeptide repeat protein SKI3|SKI5 Ski complex component and TPR protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, TTC37, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome RNA catabolic process molecular_function cytoplasm 5604 BY4741 16_5 26 B 2 YPR191W QCR2 ubiquinol--cytochrome-c reductase subunit 2|UCR2|COR2 Subunit 2 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 5606 BY4741 16_5 "slow growth, petite" 26 B 3 YPR192W AQY1 - Spore-specific water channel; mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance meiotic cell cycle, sporulation, transmembrane transport transmembrane transporter activity membrane, plasma membrane 5607 BY4741 16_5 26 B 4 YPR193C HPA2 KAT10 Tetrameric histone acetyltransferase; has similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity; also acetylates polyamines peptidyl-amino acid modification, protein acylation, chromatin organization, protein complex biogenesis, histone modification transferase activity cytoplasm 5608 BY4741 16_5 26 B 5 YPR194C OPT2 - Oligopeptide transporter; localized to peroxisomes and affects glutathione redox homeostasis; also localizes to the plasma membrane (PM) and to the late Golgi, and has a role in maintenance of lipid asymmetry between the inner and outer leaflets of the PM; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles and in polarized cell growth vacuole organization, cell morphogenesis, regulation of organelle organization, ion transport, regulation of transport, organelle fusion, membrane fusion, lipid transport other peroxisome, endomembrane system, membrane, Golgi apparatus, cytoplasm, plasma membrane 5609 BY4741 16_5 26 B 6 YPR195C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 5610 BY4741 16_5 26 B 7 YPR196W - putative maltose-responsive transcription factor Putative maltose-responsive transcription factor other DNA binding cellular_component 5611 BY4741 16_5 26 B 8 YPR197C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SGE1/YPR198W biological_process molecular_function cellular_component 5612 BY4741 16_5 26 B 9 YPR198W SGE1 NOR1 Plasma membrane multidrug transporter; member of the major facilitator superfamily; acts as an extrusion permease; partial multicopy suppressor of gal11 mutations response to chemical transmembrane transporter activity membrane, plasma membrane 5613 BY4741 16_5 26 B 10 YPR199C ARR1 ACR1|YAP8 Transcriptional activator of the basic leucine zipper (bZIP) family; required for transcription of genes involved in resistance to arsenic compounds response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 5614 BY4741 16_5 26 B 11 YPR200C ARR2 ACR2 Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p response to chemical oxidoreductase activity cellular_component 5615 BY4741 16_5 26 B 12 YPR201W ARR3 ACR3 Plasma membrane metalloid/H+ antiporter; member of bile/arsenite/riboflavin transporter (BART) superfamily; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite ion transport transmembrane transporter activity membrane, plasma membrane 5616 BY4741 16_5 26 C 1 YCR090C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene biological_process molecular_function nucleus, cytoplasm 5809 BY4741 00_2 26 C 3 YCR091W KIN82 putative serine/threonine protein kinase KIN82|FPK2 Putative serine/threonine protein kinase; implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases); involved in the phosphorylation of upstream inhibitory kinase Ypk1p along with Fpk1p; has a redundant role in the cellular response to mating pheromone; KIN82 has a paralog, FPK1, that arose from the whole genome duplication response to chemical, protein phosphorylation, ion transport, lipid transport, conjugation kinase activity, transferase activity cellular_component 5810 BY4741 00_2 26 C 4 YCR092C MSH3 mismatch repair protein MSH3 Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability meiotic cell cycle, DNA repair, organelle fission, DNA recombination, cellular response to DNA damage stimulus DNA binding nucleus, cytoplasm 5811 BY4741 00_2 26 C 5 YCR094W CDC50 aminophospholipid translocase regulatory protein CDC50 Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication ion transport, lipid transport, endocytosis hydrolase activity, ATPase activity endomembrane system, membrane, Golgi apparatus, cytoplasm 5813 BY4741 00_2 26 C 6 YCR098C GIT1 - Plasma membrane permease; mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability transmembrane transport, ion transport transmembrane transporter activity membrane, plasma membrane 5815 BY4741 00_2 26 C 7 YCR099C - - Putative protein of unknown function biological_process molecular_function membrane 5816 BY4741 00_2 26 C 8 YCR100C - - Putative protein of unknown function biological_process molecular_function membrane 5817 BY4741 00_2 26 C 9 YCR101C - - Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene biological_process molecular_function membrane 5818 BY4741 00_2 26 C 10 YCR102C - - Putative protein of unknown function; involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family response to chemical molecular_function cellular_component 5819 BY4741 00_2 26 C 11 YCR105W ADH7 ADHVII NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance other oxidoreductase activity cellular_component 5821 BY4741 00_2 26 C 12 YCR106W RDS1 - Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide response to chemical DNA binding cellular_component 5822 BY4741 00_2 26 D 1 YDL130W-A STF1 AIS2 Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress; STF1 has a paralog, INH1, that arose from the whole genome duplication other enzyme binding mitochondrion, cytoplasm 5823 BY4741 00_2 26 D 2 YDR363W-A SEM1 proteasome regulatory particle lid subunit SEM1|HOD1|DSS1 Component of lid subcomplex of 26S proteasome regulatory subunit; involved in mRNA export mediated by TREX-2 complex (Sac3p-Thp1p); assumes different conformations in different contexts, functions as molecular glue stabilizing the Rpn3p/Rpn7p regulatory heterodimer, and tethers it to lid helical bundle; ortholog of human DSS1; protein abundance increases in response to DNA replication stress chromatin organization, proteolysis involved in cellular protein catabolic process, nucleobase-containing compound transport, protein complex biogenesis, histone modification, protein modification by small protein conjugation or removal, exocytosis, nuclear transport, regulation of cell cycle molecular_function nucleus, cytoplasm 5828 BY4741 00_2 26 D 3 YDR525W-A SNA2 - Protein of unknown function; has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function membrane, vacuole, cytoplasm 5829 BY4741 00_2 26 D 4 YDR535C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene biological_process molecular_function cellular_component 5830 BY4741 00_2 26 D 5 YDR536W STL1 glucose-inactivated glycerol proton symporter STL1 Glycerol proton symporter of the plasma membrane; subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock transmembrane transport, carbohydrate transport transmembrane transporter activity membrane, plasma membrane 5831 BY4741 00_2 26 D 6 YDR538W PAD1 phenylacrylic acid decarboxylase PAD1|POF1 Phenylacrylic acid decarboxylase; confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX; co-overproduction of Pad1p and Fdc1p greatly increases cinnamic acid decarboxylase activity monocarboxylic acid metabolic process lyase activity, RNA binding, mRNA binding mitochondrion, cytoplasm 5833 BY4741 00_2 26 D 7 YDR539W FDC1 putative phenylacrylic acid decarboxylase FDC1 Ferulic acid decarboxylase, also active on p-coumaric acid; essential for decarboxylation of aromatic carboxylic acids to corresponding vinyl derivatives; co-overproduction of Pad1p and Fdc1p greatly increases cinnamic acid decarboxylase activity; structure implicates Glu285 as the general base in the nonoxidative decarboxylation reaction catalyzed by Fdc1p; homolog of E. coli UbiD; GFP-fusion protein localizes to cytoplasm monocarboxylic acid metabolic process lyase activity cytoplasm 5834 BY4741 00_2 26 D 8 YDR540C IRC4 - Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus DNA recombination molecular_function nucleus, cytoplasm 5835 BY4741 00_2 26 D 9 YDR541C - carbonyl reductase (NADPH-dependent) Aldehyde reductase; substrates are both aromatic and aliphatic aldehydes; uses NADPH as cofactor response to chemical oxidoreductase activity cellular_component 5836 BY4741 00_2 26 D 10 YER039C-A - - Putative protein of unknown function; YER039C-A is not an essential gene biological_process molecular_function membrane 5838 BY4741 00_2 26 D 11 YER091C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 5841 BY4741 00_2 26 D 12 YER144C UBP5 putative ubiquitin-specific protease UBP5 Putative ubiquitin-specific protease; concentrates at the bud neck; UBP5 has a paralog, DOA4, that arose from the whole genome duplication protein modification by small protein conjugation or removal peptidase activity, hydrolase activity cellular bud, site of polarized growth 5842 BY4741 00_2 26 E 1 YER188W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p biological_process molecular_function cellular_component 5843 BY4741 00_2 26 E 2 YFL034C-A RPL22B ribosomal 60S subunit protein L22B|L22e|rp4|l1c|YL31|L22B|YFL035C-B Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 5844 BY4741 00_2 26 E 3 YFR032C RRT5 - Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation biological_process molecular_function membrane 5845 BY4741 00_2 26 E 4 YFR032C-A RPL29 ribosomal 60S subunit protein L29|L29e|YL43|L29 Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 5846 BY4741 00_2 26 E 5 YFR033C QCR6 ubiquinol--cytochrome-c reductase subunit 6|UCR6|COR3 Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process transmembrane transporter activity, oxidoreductase activity nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm 5847 BY4741 00_2 26 E 6 YFR034C PHO4 phosphate-sensing transcription factor PHO4|phoD Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p; function is regulated by phosphorylation at multiple sites and by phosphate availability chromatin organization, response to starvation, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 5848 BY4741 00_2 26 E 7 YFR035C - - Putative protein of unknown function; deletion mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component 5849 BY4741 00_2 26 E 8 YFR036W CDC26 anaphase promoting complex subunit CDC26|SCD26|HIT3 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; relocalizes to the cytosol in response to hypoxia chromatin organization, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process transferase activity nucleus, cytoplasm 5850 BY4741 00_2 26 E 9 YFR038W IRC5 - Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci DNA recombination ATPase activity, hydrolase activity cellular_component 5852 BY4741 00_2 Incorrect. 26 E 10 YFR040W SAP155 - Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress; SAP155 has a paralog, SAP4, that arose from the whole genome duplication mitotic cell cycle molecular_function cytoplasm 5854 BY4741 00_2 26 E 11 YFR041C ERJ5 - Type I membrane protein with a J domain; required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response protein folding molecular_function endomembrane system, endoplasmic reticulum, cytoplasm 5855 BY4741 00_2 26 E 12 YFR043C IRC6 - Clathrin coat accessory factor; involved in clathrin-mediated vesicle trafficking; may function to link the AP-1 clathrin adaptor complex with the Rab GTPase Ypt31p; has structural similarity to G-proteins; mouse homolog Aagab (p34) functionally complements irc6 null mutation; null mutant displays increased levels of spontaneous Rad52p foci other protein binding, bridging cellular_component 5857 BY4741 00_2 26 F 1 YFR044C DUG1 metallodipeptidase Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p); human homolog CNDP2 can complement yeast dug1 mutant other peptidase activity, hydrolase activity ribosome, mitochondrion, cytoplasm 5858 BY4741 00_2 26 F 2 YFR045W - - Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white other other membrane, mitochondrial envelope, mitochondrion, cytoplasm 5859 BY4741 00_2 26 F 3 YFR046C CNN1 centromere-binding protein CNN1 Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T regulation of organelle organization, organelle assembly, protein complex biogenesis, chromosome segregation, regulation of cell cycle DNA binding nucleus, chromosome 5860 BY4741 00_2 26 F 4 YFR047C BNA6 nicotinate-nucleotide diphosphorylase (carboxylating)|QPT1 Quinolinate phosphoribosyl transferase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p cofactor metabolic process, nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups, transferase activity nucleus, cytoplasm 5861 BY4741 00_2 26 F 5 YFR048W RMD8 - Cytosolic protein required for sporulation biological_process molecular_function cytoplasm 5862 BY4741 00_2 26 F 6 YFR049W YMR31 KGD4|mitochondrial 37S ribosomal protein YMR31 Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase; recruits E3 subunit (Lpd1p) to the E1-E2 (Kgd1p, Kgd2p) core; has similarity to human mitochondrial ribosomal protein MRP-S36 mitochondrial translation, mitochondrion organization, generation of precursor metabolites and energy, cellular respiration structural constituent of ribosome, oxidoreductase activity, structural molecule activity ribosome, mitochondrion, cytoplasm 5863 BY4741 00_2 26 F 7 YFR053C HXK1 hexokinase 1 Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication carbohydrate metabolic process, cofactor metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, carbohydrate transport, nucleobase-containing small molecule metabolic process kinase activity, transferase activity mitochondrion, cytoplasm 5867 BY4741 00_2 26 F 8 YFR054C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 5868 BY4741 00_2 26 F 9 YFR055W IRC7 cysteine-S-conjugate beta-lyase IRC7 Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner monocarboxylic acid metabolic process, cellular amino acid metabolic process lyase activity cellular_component 5869 BY4741 00_2 26 F 10 YFR056C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W biological_process molecular_function cellular_component 5870 BY4741 00_2 26 F 11 YFR057W - - Putative protein of unknown function biological_process molecular_function cellular_component 5871 BY4741 00_2 26 F 12 YGR220C MRPL9 mitochondrial 54S ribosomal protein YmL9|YmL9 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 5873 BY4741 00_2 slow growth 26 G 1 YGR221C TOS2 - Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p; TOS2 has a paralog, SKG6, that arose from the whole genome duplication cell budding molecular_function cellular bud, site of polarized growth 5874 BY4741 00_2 26 G 2 YGR223C HSV2 - Phosphatidylinositol 3,5-bisphosphate-binding protein; plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization membrane invagination ion binding, lipid binding membrane, endomembrane system, vacuole, cytoplasm 5876 BY4741 00_2 26 G 3 YGR224W AZR1 - Plasma membrane transporter of the major facilitator superfamily; involved in resistance to azole drugs such as ketoconazole and fluconazole other other membrane, plasma membrane 5877 BY4741 00_2 26 G 4 YGR225W AMA1 SPO70 Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis; prevents premature assembly of the meiosis I spindle, required for DSB induced prophase I arrest meiotic cell cycle, organelle fission, sporulation, cell wall organization or biogenesis, chromosome segregation enzyme regulator activity nucleus 5878 BY4741 00_2 26 G 5 YGR226C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W biological_process molecular_function cellular_component 5879 BY4741 00_2 26 G 6 YGR227W DIE2 dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-dolichol alpha-1,2- glucosyltransferase|ALG10 Dolichyl-phosphoglucose-dependent alpha-1,2-glucosyltransferase; located in the ER; functions in pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation; has a role in regulation of ITR1 and INO1; human homolog ALG10B can complement yeast die2 null mutant carbohydrate metabolic process, protein glycosylation, lipid metabolic process transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 5880 BY4741 00_2 26 G 7 YGR228W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C biological_process molecular_function membrane 5881 BY4741 00_2 26 G 8 YGR230W BNS1 - Protein of unknown function; overexpression bypasses need for Spo12p, but not required for meiosis; BNS1 has a paralog, SPO12, that arose from the whole genome duplication meiotic cell cycle, organelle fission molecular_function cellular_component 5883 BY4741 00_2 26 G 9 YGR231C PHB2 prohibitin subunit PHB2 Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR) protein folding, organelle inheritance, mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 5884 BY4741 00_2 26 G 10 YGR232W NAS6 - Assembly chaperone for the 19S proteasome regulatory particle base; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 protein complex biogenesis molecular_function nucleus, cytoplasm 5885 BY4741 00_2 26 G 11 YGR233C PHO81 VAC6|phoS Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress other enzyme regulator activity nucleus, cytoplasm 5886 BY4741 00_2 26 G 12 YGR234W YHB1 YHB4 Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress response to chemical, response to oxidative stress oxidoreductase activity nucleus, mitochondrion, cytoplasm 5887 BY4741 00_2 26 H 1 YGR235C MIC26 MOS2|MCS29|MIO27 Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic26p is a non-essential component of the complex mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm 5888 BY4741 00_2 26 H 3 YGR236C SPG1 - Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm 5889 BY4741 00_2 26 H 4 YGR237C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm 5890 BY4741 00_2 26 H 5 YGR240C PFK1 6-phosphofructokinase subunit alpha Alpha subunit of heterooctameric phosphofructokinase; involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes carbohydrate metabolic process, cofactor metabolic process, ion transport, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, nucleobase-containing small molecule metabolic process kinase activity, transferase activity mitochondrion, cytoplasm 5893 BY4741 00_2 26 H 6 YGR241C YAP1802 - Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication endocytosis other membrane, cell cortex, cytoskeleton, cytoplasm 5894 BY4741 00_2 26 H 7 YGR242W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C biological_process molecular_function ribosome, cytoplasm 5895 BY4741 00_2 26 H 8 YGR243W MPC3 pyruvate transporter MPC3|FMP43 Highly conserved subunit of the mitochondrial pyruvate carrier (MPC); expressed during growth on nonfermentable carbon sources, and heterodimerizes with Mpc1p to form the respiratory isoform of MPC; MPC localizes to the mitochondrial inner membrane and mediates pyruvate uptake; MPC3 paralog, MPC2, heterodimerizes with Mpc1p to form the fermentative MPC isoform; protein abundance increases in response to DNA replication stress transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 5896 BY4741 00_2 26 H 9 YGR244C LSC2 succinate--CoA ligase (GDP-forming) subunit beta Beta subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration ligase activity mitochondrion, cytoplasm 5897 BY4741 00_2 26 H 10 YGR247W CPD1 2',3'-cyclic-nucleotide 3'-phosphodiesterase Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress nucleobase-containing small molecule metabolic process hydrolase activity cytoplasmic membrane-bounded vesicle, cytoplasm 5900 BY4741 00_2 26 H 11 YGR249W MGA1 - Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants other nucleic acid binding transcription factor activity, DNA binding nucleus 5902 BY4741 00_2 26 H 12 YBL001C ECM15 - Non-essential protein of unknown function; likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis biological_process molecular_function nucleus, cytoplasm 3025 BY4741 02_1 27 A 1 YOL152W FRE7 putative ferric-chelate reductase Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels ion transport oxidoreductase activity membrane, plasma membrane 7392 BY4741 00_18 27 A 2 YBL003C HTA2 histone H2A|H2A2 Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p DNA repair, chromatin organization, cellular response to DNA damage stimulus DNA binding nucleus, chromosome 3027 BY4741 02_1 27 A 3 YBL005W PDR3 drug-responsive transcription factor PDR3|TPE2|AMY2 Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting PDRE sites (PDR responsive elements); has a role in response to drugs and organic solvents; post-translationally up-regulated in cells lacking functional mitochondrial genome; involved in diauxic shift; relative distribution to nucleus increases upon DNA replication stress; APCC(Cdh1) substrate response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity nucleus, cytoplasm 3029 BY4741 02_1 27 A 4 YBL006C LDB7 RSC14 Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions chromatin organization, cell wall organization or biogenesis, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation ATPase activity, hydrolase activity nucleus 3032 BY4741 02_1 27 A 5 YBL007C SLA1 cytoskeletal protein-binding protein SLA1 Cytoskeletal protein binding protein; required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains cytoskeleton organization, protein complex biogenesis, cell wall organization or biogenesis, endocytosis protein binding, bridging, ubiquitin-like protein binding nucleus, cell cortex, cytoskeleton, site of polarized growth, cytoplasm 3033 BY4741 02_1 27 A 6 YBL008W HIR1 - Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores chromatin organization, protein complex biogenesis, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation chromatin binding, DNA binding, transcription factor activity, protein binding nucleus, chromosome 3034 BY4741 02_1 27 A 7 YBL009W ALK2 protein kinase ALK2 Protein kinase; along with its paralog, ALK1, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of cell polarity factors in mitosis, proper localization of formins and polarity factors, and survival in cells that activate spindle assembly checkpoint; phosphorylated in response to DNA damage; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins mitotic cell cycle, meiotic cell cycle, protein phosphorylation, organelle fission kinase activity, transferase activity cellular_component 3035 BY4741 02_1 27 A 8 YBL010C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles biological_process molecular_function cytoplasmic membrane-bounded vesicle, cytoplasm 3036 BY4741 02_1 27 A 9 YBL011W SCT1 bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase SCT1|GAT2 Glycerol 3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; dual substrate-specific acyltransferase of the glycerolipid biosynthesis pathway; prefers 16-carbon fatty acids; similar to Gpt2p; gene is constitutively transcribed lipid metabolic process transferase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 3037 BY4741 02_1 27 A 10 YBL012C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 3038 BY4741 02_1 "slow growth, petite" 27 A 11 YBL013W FMT1 - Methionyl-tRNA formyltransferase; catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate tRNA processing, RNA modification transferase activity mitochondrion, cytoplasm 3039 BY4741 02_1 27 A 12 YBL015W ACH1 - Protein with CoA transferase activity; particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth monocarboxylic acid metabolic process transferase activity, hydrolase activity mitochondrion, cytoplasm 3041 BY4741 02_1 27 B 1 YBL016W FUS3 mitogen-activated serine/threonine-protein kinase FUS3|DAC2 Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its; inhibits recruitment of Ste5p, Cdc42p-mediated asymmetry and mating morphogenesis response to chemical, regulation of transport, conjugation, invasive growth in response to glucose limitation, protein phosphorylation, transposition, regulation of protein modification process, nuclear transport, regulation of cell cycle, signaling signal transducer activity, kinase activity, transferase activity nucleus, mitochondrion, site of polarized growth, cytoplasm 3042 BY4741 02_1 27 B 2 YBL017C PEP1 VPT1|VPS10 Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments protein targeting other membrane, endomembrane system, cytoplasm, Golgi apparatus 3043 BY4741 02_1 27 B 3 YBL019W APN2 DNA-(apurinic or apyrimidinic site) lyase APN2|ETH1 Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII DNA repair, cellular response to DNA damage stimulus lyase activity, nuclease activity, hydrolase activity nucleus 3045 BY4741 02_1 27 B 4 YBL021C HAP3 - Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding response to chemical, transcription from RNA polymerase II promoter, generation of precursor metabolites and energy, cellular respiration nucleic acid binding transcription factor activity, DNA binding nucleus 3047 BY4741 02_1 "slow growth, petite" 27 B 5 YBL022C PIM1 ATP-dependent Lon protease PIM1|LON1 ATP-dependent Lon protease; involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria proteolysis involved in cellular protein catabolic process, protein complex biogenesis peptidase activity, ATPase activity, hydrolase activity mitochondrion, cytoplasm 3048 BY4741 02_1 "slow growth, petite" 27 B 6 YBL024W NCL1 tRNA (cytosine-5-)-methyltransferase|TRM4 S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase; methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 response to chemical, tRNA processing, response to oxidative stress, RNA modification RNA binding, transferase activity, methyltransferase activity nucleus 3050 BY4741 02_1 27 B 7 YBL027W RPL19B ribosomal 60S subunit protein L19B|L19e|rpl5L|YL14|L23B|L19B Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 3053 BY4741 02_1 27 B 8 YBL028C - - Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis biological_process molecular_function nucleus, nucleolus, ribosome, cytoplasm 3054 BY4741 02_1 27 B 9 YBL029W - - Non-essential protein of unknown function biological_process molecular_function nucleus, cytoplasm 3055 BY4741 02_1 27 B 10 YBL031W SHE1 - Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function mitotic cell cycle, cytoskeleton organization cytoskeletal protein binding nucleus, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 3057 BY4741 02_1 27 B 11 YBL032W HEK2 KHD1 RNA binding protein involved in asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K telomere organization, DNA replication RNA binding, mRNA binding nucleus, chromosome, cytoplasm 3058 BY4741 02_1 27 B 12 YBL036C - - Putative non-specific single-domain racemase; based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS biological_process ion binding cellular_component 3062 BY4741 02_1 27 C 1 YBL037W APL3 - Alpha-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport other molecular_function endomembrane system, cellular bud, cytoplasmic membrane-bounded vesicle, membrane, site of polarized growth, cytoplasm, plasma membrane 3063 BY4741 02_1 27 C 2 YBL038W MRPL16 RML16|mitochondrial 54S ribosomal protein YmL47|YmL47 Mitochondrial ribosomal protein of the large subunit; homologous to bacterial L16 ribosomal protein; synthetic lethality with hac1 mutation suggests a possible role in synthesis of precursors for protein glycosylation mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm 3064 BY4741 02_1 "slow growth, petite" 27 C 4 YBL039C URA7 CTP synthase URA7 Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication lipid metabolic process, nucleobase-containing small molecule metabolic process ligase activity cytoplasm 3065 BY4741 02_1 27 C 5 YBL042C FUI1 - High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, plasma membrane 3068 BY4741 02_1 27 C 6 YBL043W ECM13 - Non-essential protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; ECM13 has a paralog, YJR115W, that arose from the whole genome duplication biological_process molecular_function cellular_component 3069 BY4741 02_1 27 C 7 YBL044W - - Putative protein of unknown function; YBL044W is not an essential protein biological_process molecular_function cellular_component 3070 BY4741 02_1 "slow growth, petite" 27 C 8 YBL045C COR1 ubiquinol--cytochrome-c reductase subunit COR1|QCR1 Core subunit of the ubiquinol-cytochrome c reductase complex; the ubiquinol-cytochrome c reductase complex (bc1 complex) is a component of the mitochondrial inner membrane electron transport chain generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm 3071 BY4741 02_1 "slow growth, petite" 27 C 9 YBL046W PSY4 HSM6 Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 protein dephosphorylation, cellular response to DNA damage stimulus, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm 3072 BY4741 02_1 27 C 10 YBL047C EDE1 BUD15 Scaffold protein involved in the formation of early endocytic sites; putative regulator of cytokinesis; homo-oligomerization is required for localization to and organization of endocytic sites; has a network of interactions with other endocytic proteins; binds membranes in a ubiquitin-dependent manner; may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death; homolog of mammalian Eps15 response to chemical, cytokinesis, signaling, regulation of cell cycle, endocytosis ubiquitin-like protein binding cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm 3073 BY4741 02_1 27 C 11 YBL048W RRT1 - Protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data biological_process molecular_function membrane 3074 BY4741 02_1 27 C 12 YBL049W MOH1 - Protein of unknown function, essential for stationary phase survival; not required for growth on nonfermentable carbon sources; possibly linked with vacuolar transport biological_process molecular_function cellular_component 3075 BY4741 02_1 27 D 1 YBL051C PIN4 MDT1 Protein involved in G2/M phase progression and response to DNA damage; interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage mitotic cell cycle, cellular response to DNA damage stimulus RNA binding, mRNA binding cytoplasm 3077 BY4741 02_1 27 D 2 YBL052C SAS3 KAT6 Histone acetyltransferase catalytic subunit of NuA3 complex; acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity nucleus 3078 BY4741 02_1 27 D 3 YBL053W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 3079 BY4741 02_1 27 D 4 YBL054W TOD6 PBF1 PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; activated in stochastic pulses of nuclear localization transcription from RNA polymerase II promoter DNA binding nucleus, chromosome, cytoplasm 3080 BY4741 02_1 27 D 5 YBL055C - 3'-5'-exodeoxyribonuclease|Tat-D 3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases response to chemical, response to oxidative stress nuclease activity, hydrolase activity cytoplasm 3081 BY4741 02_1 27 D 6 YBL056W PTC3 type 2C protein phosphatase PTC3 Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication protein phosphorylation, protein dephosphorylation, regulation of protein modification process, signaling, response to osmotic stress, regulation of cell cycle phosphatase activity, hydrolase activity nucleus, cytoplasm 3082 BY4741 02_1 27 D 7 YBL057C PTH2 aminoacyl-tRNA hydrolase One of two mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth; see also PTH1 proteolysis involved in cellular protein catabolic process hydrolase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm 3083 BY4741 02_1 27 D 8 YBL058W SHP1 protein phosphatase regulator SHP1|UBX1 UBX (ubiquitin regulatory X) domain-containing protein; regulates Glc7p phosphatase activity; shp1 mutants are impaired in growth and mitotic progression; functions in growth and mitotic progression require Cdc48p binding; mitotic phenotype is caused by reduced Glc7p activity; interacts with ubiquitylated proteins, required for degradation of a ubiquitylated model substrate vacuole organization, meiotic cell cycle, carbohydrate metabolic process, response to chemical, sporulation, cytoskeleton organization, organelle assembly, proteolysis involved in cellular protein catabolic process, generation of precursor metabolites and energy, chromosome segregation, membrane invagination enzyme regulator activity nucleus, cytoplasm 3084 BY4741 02_1 27 D 9 YBL059W - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication biological_process molecular_function mitochondrion, cytoplasm 3085 BY4741 02_1 27 D 10 YBL060W YEL1 Arf family guanine nucleotide exchange factor YEL1 Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip lipid metabolic process guanyl-nucleotide exchange factor activity cellular bud, site of polarized growth, cytoplasm 3086 BY4741 02_1 27 D 11 YBL061C SKT5 CSD4|CHS4|CAL2 Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication carbohydrate metabolic process, cell wall organization or biogenesis enzyme regulator activity cellular bud, cell cortex, site of polarized growth, cytoskeleton, cytoplasm 3087 BY4741 02_1 27 D 12 YBL062W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 3088 BY4741 02_1 27 E 1 YBL063W KIP1 CIN9 Kinesin-related motor protein; required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions mitotic cell cycle, organelle fission, cytoskeleton organization, organelle assembly, protein complex biogenesis, chromosome segregation hydrolase activity, structural molecule activity nucleus, cytoskeleton, microtubule organizing center 3089 BY4741 02_1 27 E 2 YBL064C PRX1 thioredoxin peroxidase PRX1 Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress response to chemical, response to oxidative stress oxidoreductase activity mitochondrion, cytoplasm 3090 BY4741 02_1 27 E 3 YBL065W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene biological_process molecular_function cellular_component 3091 BY4741 02_1 27 E 4 YBL066C SEF1 - Putative transcription factor; has homolog in Kluyveromyces lactis biological_process molecular_function cellular_component 3092 BY4741 02_1 27 E 5 YBL067C UBP13 ubiquitin-specific protease UBP13 Ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication other peptidase activity, hydrolase activity cellular_component 3093 BY4741 02_1 27 E 6 YBL068W PRS4 ribose phosphate diphosphokinase subunit PRS4 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP; which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication; a missense mutation in the conserved residue R196 of its human homolog PRPS1 is pathogenic cell wall organization or biogenesis transferase activity cytoplasm 3094 BY4741 02_1 27 E 7 YBL069W AST1 - Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication protein targeting molecular_function membrane, endomembrane system, plasma membrane, cytoplasm, Golgi apparatus 3095 BY4741 02_1 27 E 8 YBL070C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 3096 BY4741 02_1 27 E 9 YBL071C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component 3097 BY4741 02_1 27 E 10 YBL072C RPS8A ribosomal 40S subunit protein S8A|S8e|rp19|YS9|S8A|S14A Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 3098 BY4741 02_1 27 E 11 YBL075C SSA3 Hsp70 family ATPase SSA3|YG106 ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication protein targeting, transmembrane transport, proteolysis involved in cellular protein catabolic process, protein folding hydrolase activity, unfolded protein binding, ATPase activity cytoplasm 3101 BY4741 02_1 27 E 12 YBL078C ATG8 ubiquitin-like protein ATG8|AUT7|CVT5|APG8 Component of autophagosomes and Cvt vesicles; regulator of Atg1p, targets it to autophagosomes; binds the Atg1p-Atg13p complex, triggering its vacuolar degradation; unique ubiquitin-like protein whose conjugation target is lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis vacuole organization, protein targeting, mitochondrion organization, organelle assembly, Golgi vesicle transport, membrane fusion, membrane invagination other vacuole, membrane, cytoplasm 3104 BY4741 02_1 27 F 1 YBL079W NUP170 NLE3 Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication protein targeting, chromatin organization, protein complex biogenesis, chromosome segregation, nuclear transport, nucleus organization structural molecule activity nucleus, endomembrane system, membrane 3105 BY4741 02_1 27 F 2 YBL080C PET112 glutamyl-tRNA(Gln) amidotransferase subunit PET112 Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog mitochondrial translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm 3106 BY4741 02_1 "slow growth, petite" 27 F 3 YBL081W - - Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport biological_process molecular_function cellular_component 3107 BY4741 02_1 27 F 4 YBL082C ALG3 dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase|RHK1 Dolichol-P-Man dependent alpha(1-3) mannosyltransferase; involved in synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins; G353A missense mutation in human ortholog ALG3 implicated in carbohydrate deficient glycoprotein syndrome type IV, which is characterized by microcephaly, severe epilepsy, minimal psychomotor development, partial deficiency of sialic acids in serum glycoproteins; wild-type human ALG3 can complement yeast alg3 mutant carbohydrate metabolic process, protein glycosylation, lipid metabolic process transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm 3108 BY4741 02_1 27 F 5 YBL083C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 biological_process molecular_function cellular_component 3109 BY4741 02_1 27 F 6 YBL085W BOI1 BOB1|GIN7 Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication cell budding lipid binding, ion binding cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane 3111 BY4741 02_1 27 F 7 YBL086C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function cellular_component 3112 BY4741 02_1 27 F 8 YBL087C RPL23A ribosomal 60S subunit protein L23A|L14|YL32|L23A|L17aA Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm 3113 BY4741 02_1 27 F 9 YBL088C TEL1 DNA-binding protein kinase TEL1 Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; acts with Red1p and Mec1p to promote interhomolog recombination by phosphorylation of Hop1; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene DNA repair, telomere organization, protein phosphorylation, chromatin organization, cellular response to DNA damage stimulus, histone modification kinase activity, transferase activity, DNA binding nucleus, mitochondrion, cytoplasm 3114 BY4741 02_1 27 F 10 YBL089W AVT5 - Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication other other membrane 3115 BY4741 02_1 27 F 11 YBL090W MRP21 mitochondrial 37S ribosomal protein MRP21|MRP50 Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences translational initiation, mitochondrial translation, mitochondrion organization structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm 3116 BY4741 02_1 "slow growth, petite" 27 F 12 YBL091C MAP2 methionine aminopeptidase Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p protein maturation peptidase activity, hydrolase activity nucleus, cytoplasm 3117 BY4741 02_1 27 G 1 YPL158C AIM44 GPS1 Protein that regulates Cdc42p and Rho1p; functions in the late steps of cytokinesis and cell separation; sustains Rho1p at the cell division site after actomyosin ring contraction; inhibits the activation of Cdc42-Cla4 at the cell division site to prevent budding inside the old bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress mitotic cell cycle, cytokinesis molecular_function cellular bud, site of polarized growth 7394 BY4741 00_18 27 G 2 YBL094C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W biological_process molecular_function cellular_component 3120 BY4741 02_1 27 G 3 YGL002W ERP6 - Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication Golgi vesicle transport molecular_function membrane, mitochondrion, cytoplasm 4370 BY4741 07_1 27 G 4 YGL003C CDH1 HCT1 Activator of anaphase-promoting complex/cyclosome (APC/C); cell-cycle regulated; directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p mitotic cell cycle, protein phosphorylation, organelle fission, cytoskeleton organization, regulation of organelle organization, proteolysis involved in cellular protein catabolic process, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm 4371 BY4741 07_1 27 G 5 YGL004C RPN14 - Assembly chaperone for the 19S proteasome regulatory particle base; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p proteolysis involved in cellular protein catabolic process, protein complex biogenesis molecular_function nucleus, cytoplasm 4372 BY4741 07_1 27 G 6 YGL005C COG7 Golgi transport complex subunit COG7|COD5 Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Golgi vesicle transport, protein targeting molecular_function endomembrane system, mitochondrion, cytoplasm, Golgi apparatus 4373 BY4741 07_1 27 G 7 YGL006W PMC1 calcium-transporting ATPase PMC1 Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a transmembrane transport, ion transport, cellular ion homeostasis hydrolase activity, transmembrane transporter activity, ATPase activity vacuole, membrane, cytoplasm 4374 BY4741 07_1 27 G 8 YGL007W BRP1 - Dubious ORF located in the upstream region of PMA1; deletion leads to polyamine resistance due to downregulation of PMA1 biological_process molecular_function cellular_component 4375 BY4741 07_1 27 G 9 YGL010W MPO1 - Protein involved in metabolism of phytosphingosine; not an essential gene lipid metabolic process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm 4378 BY4741 07_1 27 G 10 YGL012W ERG4 delta(24(24(1)))-sterol reductase C-24(28) sterol reductase; catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol lipid metabolic process oxidoreductase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm 4380 BY4741 07_1 "growth on -met, no growth on -lys, mates like alpha, no growth on drop-in media. PCR mating type alpha" 27 G 11 YGL013C PDR1 drug-responsive transcription factor PDR1|TPE3|TPE1|TIL1|SMR2|NRA2|CYH3|BOR2|ANT1|AMY1 Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; relocalizes to the cytosol in response to hypoxia; PDR1 has a paralog, PDR3, that arose from the whole genome duplication response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm 4381 BY4741 07_1 27 G 12 YGL014W PUF4 YGL023 Member of the PUF protein family; PUF family is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors RNA catabolic process, regulation of translation, chromatin organization, regulation of organelle organization RNA binding, mRNA binding cytoplasm 4382 BY4741 07_1 27 H 1 YGL015C - - Putative protein of unknown function; null mutants accumulate cargo in the Golgi biological_process molecular_function membrane 4383 BY4741 07_1 27 H 3 YGL016W KAP122 PDR6 Karyopherin beta; responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance protein targeting, nuclear transport protein transporter activity nucleus, membrane, endomembrane system, cytoplasm 4384 BY4741 07_1 27 H 4 YGL017W ATE1 - Arginyl-tRNA-protein transferase; catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway proteolysis involved in cellular protein catabolic process transferase activity cytoplasm 4385 BY4741 07_1 27 H 5 YGL019W CKB1 casein kinase 2 regulatory subunit CKB1 Beta regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases transcription from RNA polymerase I promoter, protein phosphorylation, transcription from RNA polymerase III promoter, cellular response to DNA damage stimulus enzyme regulator activity nucleus, nucleolus 4387 BY4741 07_1 27 H 6 YGL021W ALK1 protein kinase ALK1 Protein kinase; along with its paralog, ALK2, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of cell polarity factors in mitosis, proper localization of formins and polarity factors, and survival in cells that activate spindle assembly checkpoint; phosphorylated in response to DNA damage; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins mitotic cell cycle, protein phosphorylation, organelle fission kinase activity, transferase activity cellular_component 4389 BY4741 07_1 27 H 7 YGL023C PIB2 - Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 biological_process molecular_function mitochondrion, cytoplasm 4391 BY4741 07_1 27 H 8 YGL024W - SST3 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF PGD1/YGL025C biological_process molecular_function membrane 4392 BY4741 07_1 27 H 9 YGL025C PGD1 MED3|HRS1 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor DNA-templated transcription, initiation, transcription from RNA polymerase II promoter transcription factor activity, protein binding, transcription factor binding nucleus 4393 BY4741 07_1 27 H 10 YGL026C TRP5 tryptophan synthase TRP5 Tryptophan synthase; catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis cellular amino acid metabolic process lyase activity nucleus, cytoplasm 4394 BY4741 07_1 no growth on drop-in media 27 H 11 YGL027C CWH41 DER7|GLS1 Processing alpha glucosidase I; ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress carbohydrate metabolic process, protein glycosylation, cell wall organization or biogenesis hydrolase activity, hydrolase activity, acting on glycosyl bonds endomembrane system, membrane, endoplasmic reticulum, cytoplasm 4395 BY4741 07_1 27 H 12 YGL028C SCW11 - Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p biological_process hydrolase activity, hydrolase activity, acting on glycosyl bonds cell wall 4396 BY4741 07_1 28 A 1 YPL194W DDC1 - DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress mitotic cell cycle, meiotic cell cycle, DNA repair, organelle fission, regulation of organelle organization, cellular response to DNA damage stimulus, DNA recombination, regulation of cell cycle molecular_function nucleus, chromosome 7396 BY4741 00_18 28 A 2 YGL031C RPL24A ribosomal 60S subunit protein L24A|L24e|rp29|YL21|L30A|L24A|RPL30A Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genom