mat a Synced to SGD on Sat Jul 8 00:51:40 2017 Plate # Row Column ORF Gene Alias Description GO Biological Process GO Molecular Function GO Cellular Component background [00-1] A 1 - - - - - - - - [00-1] A 2 YAL064C-A TDA8 YAL065C-A Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene biological_process molecular_function cellular_component BY4742 [00-1] A 3 - cellular_component cellular_component cellular_component - - - - [00-1] A 4 YBL091C-A SCS22 phospholipid metabolism-regulating protein SCS22 Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect the ER and plasma membrane (PM); regulates PM PI4P levels by controlling access of the Sac1p phosphatase to its substrate, PI4P; human VAP homolog; similar to D. melanogaster inturned protein; SWAT-GFP and mCherry fusion proteins localize to the cytosol; SCS22 has a paralog, SCS2, that arose from the whole genome duplication lipid metabolic process molecular_function cytoplasm BY4742 [00-1] A 5 YBR269C SDH8 FMP21 Protein required for assembly of succinate dehydrogenase; interacts with flavinylated Sdh1p and may function as a chaperone for free Sdh1p, protecting its FAD cofactor from redox reactions before assembly of the complex; soluble protein of the mitochondrial matrix; respiratory defect of null mutant is functionally complemented by Drosophila and human orthologs mitochondrion organization, protein complex biogenesis molecular_function mitochondrion, cytoplasm BY4742 [00-1] A 6 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-1] A 7 YBR271W EFM2 S-adenosylmethionine-dependent methyltransferase S-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which dimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 613 and methylates EF3 (Yef3p) at lysine 187; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; involved in regulation of translational termination; predicted involvement in ribosome biogenesis peptidyl-amino acid modification, regulation of translation, protein alkylation transferase activity, methyltransferase activity cytoplasm BY4742 [00-1] A 8 - cytoplasm cytoplasm cytoplasm - - - - [00-1] A 9 YBR273C UBX7 CUI3 UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication sporulation, proteolysis involved in cellular protein catabolic process molecular_function nucleus, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-1] A 10 YBR274W CHK1 serine/threonine protein kinase CHK1 Serine/threonine kinase and DNA damage checkpoint effector; mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase protein phosphorylation, DNA replication, regulation of DNA metabolic process, cellular response to DNA damage stimulus, regulation of cell cycle kinase activity, transferase activity nucleus BY4742 [00-1] A 11 - nucleus nucleus nucleus - - - - [00-1] A 12 - - - - - - - - [00-1] B 1 YBR277C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W biological_process molecular_function cellular_component BY4742 [00-1] B 2 YBR278W DPB3 DNA polymerase epsilon noncatalytic subunit Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication DNA repair, chromatin organization, DNA replication, cellular response to DNA damage stimulus transferase activity, nucleotidyltransferase activity, DNA binding nucleus, chromosome BY4742 [00-1] B 3 YBR279W PAF1 - Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 transcription from RNA polymerase I promoter, peptidyl-amino acid modification, mitotic cell cycle, snoRNA processing, chromatin organization, DNA-templated transcription, termination, DNA recombination, mRNA processing, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation, DNA repair, protein phosphorylation, protein alkylation, rRNA processing, cellular response to DNA damage stimulus, histone modification, regulation of protein modification process, protein modification by small protein conjugation or removal, regulation of DNA metabolic process chromatin binding, nucleic acid binding transcription factor activity, enzyme binding, ion binding, transcription factor activity, protein binding nucleus, chromosome BY4742 [00-1] B 4 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [00-1] B 5 YBR281C DUG2 glutamine amidotransferase subunit DUG2 Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) other peptidase activity, transferase activity, hydrolase activity nucleus, cytoplasm BY4742 [00-1] B 6 YBR282W MRPL27 mitochondrial 54S ribosomal protein YmL27|YmL27 Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-1] B 7 YBR283C SSH1 - Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential protein targeting protein transporter activity, transmembrane transporter activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-1] B 8 YBR284W - metallo-dependent hydrolase superfamily protein Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4742 [00-1] B 9 YBR285W - - Putative protein of unknown function; YBR285W is not an essential gene biological_process molecular_function cellular_component BY4742 [00-1] B 10 YBR286W APE3 APY1 Vacuolar aminopeptidase Y; processed to mature form by Prb1p other peptidase activity, hydrolase activity vacuole, cytoplasm BY4742 [00-1] B 11 - vacuole, cytoplasm vacuole, cytoplasm vacuole, cytoplasm - - - - [00-1] B 12 - - - - - - - - [00-1] C 1 - - - - - - - - [00-1] C 2 YBR290W BSD2 - Heavy metal ion homeostasis protein; facilitates trafficking of Smf1p and Smf2p metal transporters to vacuole where they are degraded; acts as an adaptor protein with Rsp5p in the regulated endocytosis of Smf1p and is itself ubiquitylated by Rsp5p; controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification protein targeting, ion transport, proteolysis involved in cellular protein catabolic process other endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-1] C 3 YBR291C CTP1 - Mitochondrial inner membrane citrate transporter; member of the mitochondrial carrier family transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-1] C 4 YBR292C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-1] C 5 YBR293W VBA2 - Permease of basic amino acids in the vacuolar membrane transmembrane transport, ion transport, amino acid transport transmembrane transporter activity endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-1] C 6 - endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm - - - - [00-1] C 7 YBR295W PCA1 cation-transporting P-type ATPase PCA1|PAY2|CAD2 Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function transmembrane transport, ion transport, cellular ion homeostasis hydrolase activity, transmembrane transporter activity, ion binding, ATPase activity membrane, plasma membrane BY4742 [00-1] C 8 YBR296C PHO89 ITN1 Plasma membrane Na+/Pi cotransporter; active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification transmembrane transport, ion transport transmembrane transporter activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [00-1] C 9 YBR297W MAL33 transcription factor MAL33|MALR|MAL3R MAL-activator protein; part of complex locus MAL3; nonfunctional in genomic reference strain S288C carbohydrate metabolic process nucleic acid binding transcription factor activity nucleus BY4742 [00-1] C 10 YBR298C MAL31 maltose permease|MALT|MAL3T Maltose permease; high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C other transmembrane transporter activity membrane BY4742 [00-1] C 11 YBR300C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-1] C 12 - cellular_component cellular_component cellular_component - - - - [00-1] D 1 YCL001W-A - - Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III biological_process molecular_function cellular_component BY4742 [00-1] D 2 - cellular_component cellular_component cellular_component - - - - [00-1] D 3 YCR020W-B HTL1 - Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance chromatin organization, cellular response to DNA damage stimulus, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation chromatin binding, hydrolase activity, ATPase activity nucleus BY4742 [00-1] D 4 YCR024C SLM5 asparagine--tRNA ligase SLM5 Mitochondrial asparaginyl-tRNA synthetase mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm BY4742 [00-1] D 5 YCR024C-A PMP1 proteolipid ATPase Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication ion transport enzyme regulator activity membrane, plasma membrane BY4742 [00-1] D 6 YCR025C - - Putative protein of unknown function; conserved across S. cerevisiae strains; YCR025C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-1] D 7 YCR026C NPP1 nucleotide diphosphatase/phosphodiesterase NPP1 Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; involved in spore wall assembly; NPP1 has a paralog, NPP2, that arose from the whole genome duplication, and an npp1 npp2 double mutant exhibits reduced dityrosine fluorescence relative to the single mutants response to starvation, nucleobase-containing small molecule metabolic process nuclease activity, hydrolase activity cellular_component BY4742 [00-1] D 8 YCR027C RHB1 putative GTPase RHB1|RSG1 Putative Rheb-related GTPase; involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins ion transport, endosomal transport, amino acid transport GTPase activity, hydrolase activity membrane, cytoplasm, plasma membrane BY4742 [00-1] D 9 YCR028C FEN2 - Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress transmembrane transport, ion transport, endocytosis transmembrane transporter activity vacuole, membrane, cytoplasm, plasma membrane BY4742 [00-1] D 10 - vacuole, membrane, cytoplasm, plasma membrane vacuole, membrane, cytoplasm, plasma membrane vacuole, membrane, cytoplasm, plasma membrane - - - - [00-1] D 11 - - - - - - - - [00-1] D 12 YCR031C RPS14A uS11|ribosomal 40S subunit protein S14A|S11|rp59A|S14A|RPL59|CRY1 Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly RNA binding, structural constituent of ribosome, structural molecule activity, mRNA binding ribosome, cytoplasm BY4742 [00-1] E 1 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [00-1] E 2 - - - - - - - - [00-1] E 3 YCR034W ELO2 fatty acid elongase ELO2|FEN1|VBM2|GNS1 Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; ELO2 has a paralog, ELO1, that arose from the whole genome duplication; lethality of the elo2 elo3 double null mutation is functionally complemented by human ELOVL1 and weakly complemented by human ELOVL3 or ELOV7 monocarboxylic acid metabolic process, lipid metabolic process, endosomal transport transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-1] E 4 - endomembrane system, membrane, endoplasmic reticulum, cytoplasm endomembrane system, membrane, endoplasmic reticulum, cytoplasm endomembrane system, membrane, endoplasmic reticulum, cytoplasm - - - - [00-1] E 5 YCR036W RBK1 putative ribokinase Putative ribokinase carbohydrate metabolic process kinase activity, transferase activity, ion binding nucleus, cytoplasm BY4742 [00-1] E 6 YCR037C PHO87 SPX domain-containing inorganic phosphate transporter Low-affinity inorganic phosphate (Pi) transporter; acts upstream of Pho81p in regulation of the PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication transmembrane transport, ion transport, regulation of transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-1] E 7 YCR043C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene biological_process molecular_function site of polarized growth, endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-1] E 8 YCR044C PER1 COS16 Protein of the endoplasmic reticulum; required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 cellular ion homeostasis, lipid metabolic process, protein lipidation molecular_function endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-1] E 9 YCR045C RRT12 OSW3 Probable subtilisin-family protease; role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores meiotic cell cycle, sporulation, cell wall organization or biogenesis peptidase activity, hydrolase activity cell wall, nucleus, endomembrane system BY4742 [00-1] E 10 - cell wall, nucleus, endomembrane system cell wall, nucleus, endomembrane system cell wall, nucleus, endomembrane system - - - - [00-1] E 11 - - - - - - - - [00-1] E 12 - - - - - - - - [00-1] F 1 YCR049C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-1] F 2 YCR050C - - Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein biological_process molecular_function cellular_component BY4742 [00-1] F 3 YCR051W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene biological_process molecular_function nucleus, cytoplasm BY4742 [00-1] F 4 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-1] F 5 - - - - - - - - [00-1] F 6 - - - - - - - - [00-1] F 7 - - - - - - - - [00-1] F 8 YCR059C YIH1 - Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT regulation of protein modification process, protein phosphorylation cytoskeletal protein binding, enzyme regulator activity nucleus, ribosome, cytoplasm BY4742 [00-1] F 9 - nucleus, ribosome, cytoplasm nucleus, ribosome, cytoplasm nucleus, ribosome, cytoplasm - - - - [00-1] F 10 YCR061W - YCR062W Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation other molecular_function membrane, cytoplasm BY4742 [00-1] F 11 - membrane, cytoplasm membrane, cytoplasm membrane, cytoplasm - - - - [00-1] F 12 YCR063W BUD31 U2 snRNP complex subunit BUD31|CWC14 Component of the SF3b subcomplex of the U2 snRNP; increases efficiency of first and second step pre-mRNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis mitotic cell cycle, RNA splicing, cytokinesis, mRNA processing molecular_function nucleus BY4742 [00-1] G 1 - nucleus nucleus nucleus - - - - [00-1] G 2 YCR065W HCM1 - Forkhead transcription factor; drives S-phase activation of genes involved in chromosome segregation, spindle dynamics, budding; also activates genes involved in respiration, use of alternative energy sources (like proline), NAD synthesis, oxidative stress resistance; key factor in early adaptation to nutrient deficiency and diauxic shift; suppressor of calmodulin mutants with specific SPB assembly defects; ortholog of C. elegans lifespan regulator PHA-4 response to chemical, mitochondrion organization, cytoskeleton organization, response to oxidative stress, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, chromosome, cytoplasm BY4742 [00-1] G 3 YCR066W RAD18 E3 ubiquitin-protein ligase RAD18 E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA DNA repair, cellular response to DNA damage stimulus, protein modification by small protein conjugation or removal hydrolase activity, DNA binding, ATPase activity nucleus, chromosome BY4742 [00-1] G 4 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [00-1] G 5 YCR068W ATG15 triglyceride lipase ATG15|CVT17|AUT5 Phospholipase; preferentially hydrolyses phosphatidylserine, with minor activity against cardiolipin and phosphatidylethanolamine; required for lysis of autophagic and CVT bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway; required for the maintenance of lipid droplet quantity after the diauxic shift; regulates lipolysis; expression regulated by Yap1p during autophagy vesicle organization, protein maturation, lipid metabolic process hydrolase activity endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-1] G 6 - endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm - - - - [00-1] G 7 YCR071C IMG2 mitochondrial 54S ribosomal protein IMG2 Mitochondrial ribosomal protein of the large subunit; conserved in metazoa, with similarity to human mitochondrial ribosomal protein MRPL49 mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-1] G 8 - ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm - - - - [00-1] G 9 - - - - - - - - [00-1] G 10 YCR073W-A SOL2 YCRX13W Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication nucleobase-containing compound transport, nuclear transport molecular_function cytoplasm BY4742 [00-1] G 11 - cytoplasm cytoplasm cytoplasm - - - - [00-1] G 12 YCR076C FUB1 - Proteasome-binding protein; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene biological_process other cellular_component BY4742 [00-1] H 1 YCR077C PAT1 MRT1 Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; binds to mRNAs under glucose starvation, most often in the 3' UTR; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress; phosphorylation by PKA inhibits P body foci formation translational initiation, regulation of translation, chromatin organization, organelle assembly, chromosome segregation, RNA catabolic process chromatin binding, RNA binding, mRNA binding nucleus, chromosome, ribosome, cytoplasm BY4742 [00-1] H 2 YCR079W PTC6 type 2C protein phosphatase PTC6|PPP2|AUP1 Mitochondrial type 2C protein phosphatase (PP2C); has similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase protein dephosphorylation, mitochondrion organization phosphatase activity, hydrolase activity mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-1] H 3 YCR081W SRB8 MED12|YCR080W|SSN5|NUT6|GIG1 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression response to chemical, transcription from RNA polymerase II promoter transcription factor activity, protein binding nucleus BY4742 [00-1] H 4 YCR082W AHC2 - Component of the ADA histone acetyltransferase complex; Ahc2p and Ahc1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ahc1p to the complex peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification molecular_function nucleus, cytoplasm BY4742 [00-1] H 5 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-1] H 6 - - - - - - - - [00-1] H 7 YCR085W - - Putative protein of unknown function; conserved among S. cerevisiae strains; YCR085W is not an essential gene biological_process molecular_function cellular_component BY4742 [00-1] H 8 YCR086W CSM1 - Nucleolar protein that mediates homolog segregation during meiosis I; forms a complex with Lrs4p and then Mam1p at kinetochores; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation meiotic cell cycle, organelle fission, chromosome segregation, nucleus organization molecular_function nucleus, endomembrane system, chromosome, nucleolus BY4742 [00-1] H 9 YCR087C-A - LUG1 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene biological_process molecular_function nucleus, nucleolus BY4742 [00-1] H 10 YCR087W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene biological_process molecular_function membrane BY4742 [00-1] H 11 - membrane membrane membrane - - - - [00-1] H 12 - - - - - - - - [00-2] A 1 YCR090C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene biological_process molecular_function nucleus, cytoplasm BY4742 [00-2] A 2 YCR091W KIN82 putative serine/threonine protein kinase KIN82|FPK2 Putative serine/threonine protein kinase; implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases); involved in the phosphorylation of upstream inhibitory kinase Ypk1p along with Fpk1p; has a redundant role in the cellular response to mating pheromone; KIN82 has a paralog, FPK1, that arose from the whole genome duplication response to chemical, protein phosphorylation, ion transport, lipid transport, conjugation kinase activity, transferase activity cellular_component BY4742 [00-2] A 3 YCR092C MSH3 mismatch repair protein MSH3 Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability meiotic cell cycle, DNA repair, organelle fission, DNA replication, cellular response to DNA damage stimulus, DNA recombination, regulation of DNA metabolic process DNA binding nucleus, cytoplasm BY4742 [00-2] A 4 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-2] A 5 YCR094W CDC50 aminophospholipid translocase regulatory protein CDC50 Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication ion transport, lipid transport, endocytosis hydrolase activity, ATPase activity cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4742 [00-2] A 6 - cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm - - - - [00-2] A 7 YCR098C GIT1 - Plasma membrane permease; mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability transmembrane transport, ion transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [00-2] A 8 YCR099C - - Putative protein of unknown function biological_process molecular_function membrane BY4742 [00-2] A 9 YCR100C - - Putative protein of unknown function biological_process molecular_function membrane BY4742 [00-2] A 10 YCR101C - - Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-2] A 11 YCR102C - - Putative protein of unknown function; involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family response to chemical molecular_function cellular_component BY4742 [00-2] A 12 - cellular_component cellular_component cellular_component - - - - [00-2] B 1 YCR105W ADH7 NADP-dependent alcohol dehydrogenase|ADHVII NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance other oxidoreductase activity cellular_component BY4742 [00-2] B 2 YCR106W RDS1 - Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide response to chemical DNA binding cellular_component BY4742 [00-2] B 3 YDL130W-A STF1 ATPase-binding protein|AIS2 Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress; STF1 has a paralog, INH1, that arose from the whole genome duplication other enzyme binding mitochondrion, cytoplasm BY4742 [00-2] B 4 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-2] B 5 - - - - - - - - [00-2] B 6 - - - - - - - - [00-2] B 7 - - - - - - - - [00-2] B 8 YDR363W-A SEM1 proteasome regulatory particle lid subunit SEM1|HOD1|DSS1 19S proteasome regulatory particle lid subcomplex component; role in Ub-dependent proteolysis and proteasome stability; involved in TREX-2 mediated mRNA export, and in the prevention of transcription-associated genome instability; ubiquitinated by Nedd4-like E3-ligase, Rsp5p; human ortholog DSS1, a BRCA1 binding protein implicated in cancer, complements the yeast null; drives trinucleotide repeat expansion; protein abundance increases in response to DNA replication stress chromatin organization, proteolysis involved in cellular protein catabolic process, nucleobase-containing compound transport, protein complex biogenesis, histone modification, protein modification by small protein conjugation or removal, exocytosis, nuclear transport, regulation of cell cycle molecular_function nucleus, cytoplasm BY4742 [00-2] B 9 YDR525W-A SNA2 - Protein of unknown function; has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-2] B 10 YDR535C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-2] B 11 YDR536W STL1 glucose-inactivated glycerol proton symporter STL1 Glycerol proton symporter of the plasma membrane; subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock transmembrane transport, carbohydrate transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [00-2] B 12 - membrane, vacuole, plasma membrane, cytoplasm membrane, vacuole, plasma membrane, cytoplasm membrane, vacuole, plasma membrane, cytoplasm - - - - [00-2] C 1 YDR538W PAD1 phenylacrylic acid decarboxylase PAD1|POF1 Phenylacrylic acid decarboxylase; confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX; co-overproduction of Pad1p and Fdc1p greatly increases cinnamic acid decarboxylase activity monocarboxylic acid metabolic process lyase activity, RNA binding, mRNA binding mitochondrion, cytoplasm BY4742 [00-2] C 2 YDR539W FDC1 putative phenylacrylic acid decarboxylase FDC1 Ferulic acid decarboxylase, also active on p-coumaric acid; essential for decarboxylation of aromatic carboxylic acids to corresponding vinyl derivatives; co-overproduction of Pad1p and Fdc1p greatly increases cinnamic acid decarboxylase activity; structure implicates Glu285 as the general base in the nonoxidative decarboxylation reaction catalyzed by Fdc1p; homolog of E. coli UbiD; GFP-fusion protein localizes to cytoplasm monocarboxylic acid metabolic process lyase activity cytoplasm BY4742 [00-2] C 3 YDR540C IRC4 - Protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus DNA recombination molecular_function nucleus, cytoplasm BY4742 [00-2] C 4 YDR541C - carbonyl reductase (NADPH-dependent) Aldehyde reductase; substrates include both aromatic and aliphatic aldehydes; uses NADPH as cofactor; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively response to chemical oxidoreductase activity cellular_component BY4742 [00-2] C 5 - cellular_component cellular_component cellular_component - - - - [00-2] C 6 YER039C-A - - Putative protein of unknown function; YER039C-A is not an essential gene biological_process molecular_function membrane BY4742 [00-2] C 7 - membrane membrane membrane - - - - [00-2] C 8 - - - - - - - - [00-2] C 9 YER091C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-2] C 10 YER144C UBP5 putative ubiquitin-specific protease UBP5 Putative ubiquitin-specific protease; concentrates at the bud neck; UBP5 has a paralog, DOA4, that arose from the whole genome duplication protein modification by small protein conjugation or removal peptidase activity, hydrolase activity cellular bud, site of polarized growth BY4742 [00-2] C 11 YER188W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p biological_process molecular_function cellular_component BY4742 [00-2] C 12 YFL034C-A RPL22B eL22|ribosomal 60S subunit protein L22B|L22e|rp4|l1c|YL31|L22B|YFL035C-B Ribosomal 60S subunit protein L22A; required for translation of long 5' UTR of IME1 mRNA and meiotic entry; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-2] D 1 YFR032C RRT5 - Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation biological_process molecular_function membrane BY4742 [00-2] D 2 YFR032C-A RPL29 eL29|ribosomal 60S subunit protein L29|L29e|YL43|L29 Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-2] D 3 YFR033C QCR6 ubiquinol--cytochrome-c reductase subunit 6|UCR6|COR3 Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process transmembrane transporter activity, oxidoreductase activity nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-2] D 4 YFR034C PHO4 phosphate-sensing transcription factor PHO4|phoD Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p; function is regulated by phosphorylation at multiple sites and by phosphate availability chromatin organization, response to starvation, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [00-2] D 5 YFR035C - - Putative protein of unknown function; deletion mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component BY4742 [00-2] D 6 YFR036W CDC26 anaphase promoting complex subunit CDC26|SCD26|HIT3 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; relocalizes to the cytosol in response to hypoxia chromatin organization, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process transferase activity nucleus, cytoplasm BY4742 [00-2] D 7 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-2] D 8 YFR038W IRC5 putative ATPase Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci; SWAT-GFP and mCherry fusion proteins localize to the nucleus DNA recombination ATPase activity, hydrolase activity nucleus BY4742 [00-2] D 9 - nucleus nucleus nucleus - - - - [00-2] D 10 YFR040W SAP155 - Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress; SAP155 has a paralog, SAP4, that arose from the whole genome duplication mitotic cell cycle molecular_function cytoplasm BY4742 [00-2] D 11 YFR041C ERJ5 - Type I membrane protein with a J domain; required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response protein folding molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-2] D 12 - endomembrane system, endoplasmic reticulum, cytoplasm endomembrane system, endoplasmic reticulum, cytoplasm endomembrane system, endoplasmic reticulum, cytoplasm - - - - [00-2] E 1 YFR043C IRC6 - Clathrin coat accessory factor; involved in clathrin-mediated vesicle trafficking; may function to link the AP-1 clathrin adaptor complex with the Rab GTPase Ypt31p; has structural similarity to G-proteins; mouse homolog Aagab (p34) functionally complements irc6 null mutation; null mutant displays increased levels of spontaneous Rad52p foci other protein binding, bridging cellular_component BY4742 [00-2] E 2 YFR044C DUG1 metallodipeptidase Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p); human homolog CNDP2 can complement yeast dug1 mutant other peptidase activity, hydrolase activity ribosome, mitochondrion, cytoplasm BY4742 [00-2] E 3 YFR045W - - Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white other other membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-2] E 4 YFR046C CNN1 centromere-binding protein CNN1 Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T regulation of organelle organization, organelle assembly, protein complex biogenesis, chromosome segregation DNA binding nucleus, chromosome BY4742 [00-2] E 5 YFR047C BNA6 nicotinate-nucleotide diphosphorylase (carboxylating)|QPT1 Quinolinate phosphoribosyl transferase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p cofactor metabolic process, nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process transferase activity, transferring glycosyl groups, transferase activity nucleus, cytoplasm BY4742 [00-2] E 6 YFR048W RMD8 - Cytosolic protein required for sporulation biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-2] E 7 YFR049W YMR31 KGD4|mitochondrial 37S ribosomal protein YMR31 Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase; recruits E3 subunit (Lpd1p) to the E1-E2 (Kgd1p, Kgd2p) core; has similarity to human mitochondrial ribosomal protein MRP-S36 mitochondrial translation, mitochondrion organization, generation of precursor metabolites and energy, cellular respiration structural constituent of ribosome, oxidoreductase activity, structural molecule activity ribosome, mitochondrion, cytoplasm BY4742 [00-2] E 8 - ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm - - - - [00-2] E 9 - - - - - - - - [00-2] E 10 - - - - - - - - [00-2] E 11 YFR053C HXK1 hexokinase 1 Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication carbohydrate metabolic process, cofactor metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, carbohydrate transport, nucleobase-containing small molecule metabolic process kinase activity, transferase activity mitochondrion, cytoplasm BY4742 [00-2] E 12 YFR054C - - Putative protein of unknown function; conserved among S. cerevisiae strains biological_process molecular_function cellular_component BY4742 [00-2] F 1 YFR055W IRC7 cysteine-S-conjugate beta-lyase IRC7 Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner monocarboxylic acid metabolic process, cellular amino acid metabolic process lyase activity cellular_component BY4742 [00-2] F 2 YFR056C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W biological_process molecular_function cellular_component BY4742 [00-2] F 3 YFR057W - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-2] F 4 YGR219W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C biological_process molecular_function cellular_component BY4742 [00-2] F 5 YGR220C MRPL9 mitochondrial 54S ribosomal protein YmL9|YmL9 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-2] F 6 YGR221C TOS2 - Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p; TOS2 has a paralog, SKG6, that arose from the whole genome duplication cell budding molecular_function cellular bud, site of polarized growth BY4742 [00-2] F 7 YGR222W PET54 - Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing mitochondrial translation, RNA splicing, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis RNA binding mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-2] F 8 YGR223C HSV2 - Phosphatidylinositol 3,5-bisphosphate-binding protein; plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization other lipid binding, ion binding cytoplasmic vesicle, endomembrane system, vacuole, membrane, cytoplasm BY4742 [00-2] F 9 YGR224W AZR1 azole transporter Plasma membrane transporter of the major facilitator superfamily; involved in resistance to azole drugs such as ketoconazole and fluconazole other other membrane, plasma membrane BY4742 [00-2] F 10 YGR225W AMA1 SPO70 Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis; prevents premature assembly of the meiosis I spindle, required for DSB induced prophase I arrest meiotic cell cycle, organelle fission, sporulation, cell wall organization or biogenesis, regulation of cell cycle, chromosome segregation enzyme regulator activity nucleus BY4742 [00-2] F 11 YGR226C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W biological_process molecular_function cellular_component BY4742 [00-2] F 12 YGR227W DIE2 dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-dolichol alpha-1,2- glucosyltransferase|ALG10 Dolichyl-phosphoglucose-dependent alpha-1,2-glucosyltransferase; located in the ER; functions in pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation; has a role in regulation of ITR1 and INO1; human homolog ALG10B can complement yeast die2 null mutant protein glycosylation, lipid metabolic process transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-2] G 1 YGR228W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C biological_process molecular_function membrane BY4742 [00-2] G 2 YGR229C SMI1 KNR4 Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity mitotic cell cycle, cell wall organization or biogenesis, regulation of cell cycle molecular_function cellular bud, site of polarized growth BY4742 [00-2] G 3 YGR230W BNS1 - Protein of unknown function; overexpression bypasses need for Spo12p, but not required for meiosis; BNS1 has a paralog, SPO12, that arose from the whole genome duplication meiotic cell cycle, organelle fission molecular_function cellular_component BY4742 [00-2] G 4 YGR231C PHB2 prohibitin subunit PHB2 Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR) protein folding, organelle inheritance, mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-2] G 5 YGR232W NAS6 - Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 protein complex biogenesis molecular_function nucleus, cytoplasm BY4742 [00-2] G 6 YGR233C PHO81 VAC6|phoS Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress other enzyme regulator activity nucleus, cytoplasm BY4742 [00-2] G 7 YGR234W YHB1 flavohemoglobin|YHB4 Nitric oxide oxidoreductase; flavohemoglobin that plays role in oxidative and nitrosative stress responses; protects against nitration of cellular targets and against cell growth inhibition under aerobic or anaerobic conditions; yeast flavohemoglobin Yhb1p and human homolog neuroglobin NGB protect cells against alpha-synuclein cytotoxicity and aggregate formation; protein increases in abundance, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress response to chemical, response to oxidative stress oxidoreductase activity nucleus, mitochondrion, cytoplasm BY4742 [00-2] G 8 YGR235C MIC26 MOS2|MCS29|MIO27 Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic26p is a non-essential component of the complex mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-2] G 9 YGR236C SPG1 - Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [00-2] G 10 YGR237C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm BY4742 [00-2] G 11 - cytoplasm cytoplasm cytoplasm - - - - [00-2] G 12 - - - - - - - - [00-2] H 1 YGR240C PFK1 6-phosphofructokinase subunit alpha Alpha subunit of heterooctameric phosphofructokinase; involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes carbohydrate metabolic process, cofactor metabolic process, ion transport, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, cellular ion homeostasis, nucleobase-containing small molecule metabolic process kinase activity, transferase activity vacuole, mitochondrion, membrane, cytoplasm BY4742 [00-2] H 2 YGR241C YAP1802 - Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication endocytosis other cell cortex, cytoskeleton, cellular bud, membrane, site of polarized growth, cytoplasm BY4742 [00-2] H 3 YGR242W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C biological_process molecular_function ribosome, cytoplasm BY4742 [00-2] H 4 YGR243W MPC3 mitochondrial pyruvate carrier|FMP43 Highly conserved subunit of the mitochondrial pyruvate carrier (MPC); expressed during growth on nonfermentable carbon sources, and heterodimerizes with Mpc1p to form the respiratory isoform of MPC; MPC localizes to the mitochondrial inner membrane and mediates pyruvate uptake; MPC3 paralog, MPC2, heterodimerizes with Mpc1p to form the fermentative MPC isoform; protein abundance increases in response to DNA replication stress transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-2] H 5 YGR244C LSC2 succinate--CoA ligase (GDP-forming) subunit beta Beta subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration ligase activity mitochondrion, cytoplasm BY4742 [00-2] H 6 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-2] H 7 - - - - - - - - [00-2] H 8 YGR247W CPD1 2',3'-cyclic-nucleotide 3'-phosphodiesterase Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress nucleobase-containing small molecule metabolic process hydrolase activity cytoplasmic vesicle, cytoplasm BY4742 [00-2] H 9 - cytoplasmic vesicle, cytoplasm cytoplasmic vesicle, cytoplasm cytoplasmic vesicle, cytoplasm - - - - [00-2] H 10 YGR249W MGA1 - Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants other nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [00-2] H 11 - nucleus nucleus nucleus - - - - [00-2] H 12 - - - - - - - - [00-3] A 1 - - - - - - - - [00-3] A 2 - - - - - - - - [00-3] A 3 - - - - - - - - [00-3] A 4 YGR256W GND2 phosphogluconate dehydrogenase (decarboxylating) GND2 6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity membrane, plasma membrane, cytoplasm BY4742 [00-3] A 5 - membrane, plasma membrane, cytoplasm membrane, plasma membrane, cytoplasm membrane, plasma membrane, cytoplasm - - - - [00-3] A 6 - - - - - - - - [00-3] A 7 YGR259C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W biological_process molecular_function cellular_component BY4742 [00-3] A 8 YGR260W TNA1 - High affinity nicotinic acid plasma membrane permease; responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) transmembrane transport, ion transport transmembrane transporter activity vacuole, mitochondrion, membrane, cytoplasm, plasma membrane BY4742 [00-3] A 9 YGR261C APL6 YKS5 Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Golgi vesicle transport, protein targeting molecular_function membrane, cytoplasm BY4742 [00-3] A 10 YGR262C BUD32 serine/threonine protein kinase BUD32|LDB14 Protein kinase; component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; Pyrococcus Bud32 ortholog functions as a P-loop ATPase rather than a protein kinase in the context of the complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; mutation is functionally complemented by human TP53RK mitotic cell cycle, telomere organization, protein phosphorylation, cytokinesis, DNA recombination, transcription from RNA polymerase II promoter kinase activity, transferase activity, hydrolase activity, ATPase activity nucleus, cytoplasm BY4742 [00-3] A 11 YGR263C SAY1 steryl deacetylase Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; active both in the endoplasmic reticulum (ER) and in lipid droplets; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the ER response to chemical, lipid metabolic process hydrolase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-3] A 12 - membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm - - - - [00-3] B 1 - - - - - - - - [00-3] B 2 YGR266W - - Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, plasma membrane, cytoplasm BY4742 [00-3] B 3 - membrane, mitochondrial envelope, mitochondrion, plasma membrane, cytoplasm membrane, mitochondrial envelope, mitochondrion, plasma membrane, cytoplasm membrane, mitochondrial envelope, mitochondrion, plasma membrane, cytoplasm - - - - [00-3] B 4 YGR268C HUA1 - Cytoplasmic protein containing a zinc finger domain; sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly biological_process molecular_function cytoplasm BY4742 [00-3] B 5 YGR269W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C biological_process molecular_function cellular_component BY4742 [00-3] B 6 YGR270W YTA7 - Protein that localizes to chromatin; has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; relocalizes to the cytosol in response to hypoxia; potentially phosphorylated by Cdc28p invasive growth in response to glucose limitation, chromatin organization, transcription from RNA polymerase II promoter chromatin binding, histone binding nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-3] B 7 - nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm - - - - [00-3] B 8 - - - - - - - - [00-3] B 9 - - - - - - - - [00-3] B 10 - - - - - - - - [00-3] B 11 YGR275W RTT102 - Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress chromatin organization, transcription from RNA polymerase II promoter, chromosome segregation, DNA-templated transcription, elongation ATPase activity, hydrolase activity nucleus BY4742 [00-3] B 12 - nucleus nucleus nucleus - - - - [00-3] C 1 - - - - - - - - [00-3] C 2 - - - - - - - - [00-3] C 3 YGR279C SCW4 putative family 17 glucosidase Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating; SCW4 has a paralog, SCW10, that arose from the whole genome duplication conjugation RNA binding, mRNA binding, hydrolase activity, hydrolase activity, acting on glycosyl bonds cell wall, extracellular region, vacuole, cytoplasm BY4742 [00-3] C 4 - cell wall, extracellular region, vacuole, cytoplasm cell wall, extracellular region, vacuole, cytoplasm cell wall, extracellular region, vacuole, cytoplasm - - - - [00-3] C 5 YGR281W YOR1 ATP-binding cassette transporter YOR1|YRS1 Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter mediates export of many different organic anions including oligomycin; homolog of human cystic fibrosis transmembrane receptor (CFTR) other ATPase activity, hydrolase activity, transmembrane transporter activity membrane, plasma membrane BY4742 [00-3] C 6 YGR282C BGL2 glucan 1,3-beta-glucosidase|SCW9 Endo-beta-1,3-glucanase; major protein of the cell wall, involved in cell wall maintenance; involved in incorporation of newly synthesized mannoprotein molecules into the cell wall cell wall organization or biogenesis transferase activity, hydrolase activity, hydrolase activity, acting on glycosyl bonds cell wall, vacuole, cytoplasm BY4742 [00-3] C 7 YGR283C - putative methyltransferase Putative methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus; YGR283C has a paralog, YMR310C, that arose from the whole genome duplication other transferase activity, methyltransferase activity nucleus, nucleolus, ribosome, cytoplasm BY4742 [00-3] C 8 YGR284C ERV29 - Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Golgi vesicle transport protein binding, bridging membrane, cytoplasmic vesicle, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-3] C 9 YGR285C ZUO1 zuotin Ribosome-associated chaperone; zuotin functions in ribosome biogenesis and as a chaperone for nascent polypeptide chains in partnership with Ssz1p and SSb1/2; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p; human gene DNAJC2 can partially complement yeast zuo1 null mutant regulation of translation, rRNA processing, translational elongation, ribosomal subunit export from nucleus, protein folding, nuclear transport unfolded protein binding nucleus, ribosome, mitochondrion, nucleolus, cytoplasm BY4742 [00-3] C 10 YGR286C BIO2 biotin synthase Biotin synthase; catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant cofactor metabolic process, vitamin metabolic process, monocarboxylic acid metabolic process transferase activity mitochondrion, cytoplasm BY4742 [00-3] C 11 YGR287C IMA1 oligo-1,6-glucosidase IMA1 Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; preferred specificity for isomaltose, alpha-methylglucoside, and palatinose, but also exhibits alpha-1,2 glucosidase activity on sucrose and kojibiose, and can cleave the 1,3-alpha linkage of nigerose and turanose and the alpha-1,5 linkage of leucrose in vitro; member of the IMA isomaltase family carbohydrate metabolic process, oligosaccharide metabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds ribosome, mitochondrion, cytoplasm BY4742 [00-3] C 12 YGR288W MAL13 transcription factor MAL13|MALR MAL-activator protein; part of complex locus MAL1; nonfunctional in genomic reference strain S288C carbohydrate metabolic process nucleic acid binding transcription factor activity nucleus BY4742 [00-3] D 1 - nucleus nucleus nucleus - - - - [00-3] D 2 YGR290W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) biological_process molecular_function membrane BY4742 [00-3] D 3 - membrane membrane membrane - - - - [00-3] D 4 - - - - - - - - [00-3] D 5 - - - - - - - - [00-3] D 6 - - - - - - - - [00-3] D 7 YHR021W-A ECM12 - Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity biological_process molecular_function cellular_component BY4742 [00-3] D 8 YHR039C-B cellular_component cellular_component cellular_component - - - BY4742 [00-3] D 9 YHR079C-B - - - - - - BY4742 [00-3] D 10 YIL009C-A EST3 telomerase subunit EST3 Component of the telomerase holoenzyme; involved in telomere replication; synthesis of the full-length protein results from a programmed +1 ribosomal frameshift telomere organization GTPase activity, hydrolase activity, DNA binding, ATPase activity, helicase activity nucleus, chromosome, cytoplasm BY4742 [00-3] D 11 YIR017C MET28 - bZIP transcriptional activator in the Cbf1p-Met4p-Met28p complex; participates in the regulation of sulfur metabolism transcription from RNA polymerase II promoter, cellular amino acid metabolic process transcription factor binding nucleus BY4742 [00-3] D 12 YIR018W YAP5 - Basic leucine zipper (bZIP) iron-sensing transcription factor; involved in diauxic shift; YAP5 has a paralog, YAP7, that arose from the whole genome duplication response to chemical, chromatin organization, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity nucleus, chromosome BY4742 [00-3] E 1 YIR019C FLO11 MUC1|STA4 GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal and invasive growth, flocculation, and biofilm formation; major determinant of colony morphology; transcription regulated by the MAPK pathway (Ste12p and Tec1p) and the cAMP pathway (Flo8p); required for formation of fibrous interconnections between cells of a wild strain; role in co-flocculation with other yeast species; cleaved and shed from cells, contributing to their surface properties invasive growth in response to glucose limitation, pseudohyphal growth molecular_function vacuole, cellular bud, extracellular region, membrane, site of polarized growth, cytoplasm, plasma membrane BY4742 [00-3] E 2 YIR020C - - Protein of unknown function; mRNA identified as translated by ribosome profiling data; SWAT-GFP fusion protein localizes to the endoplasmic reticulum biological_process molecular_function cellular_component BY4742 [00-3] E 3 YIR020W-B cellular_component cellular_component cellular_component - - - BY4742 [00-3] E 4 YIR021W MRS1 PET157 Splicing protein; required for splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA; MRS1 has a paralog, CCE1, that arose from the whole genome duplication RNA splicing RNA binding mitochondrion, cytoplasm BY4742 [00-3] E 5 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-3] E 6 - - - - - - - - [00-3] E 7 YIR024C INA22 GIF1 F1F0 ATP synthase peripheral stalk assembly factor; subunit of the matrix-exposed inner mitochondrial membrane localized INA complex (Ina22p-Ina17p) involved in assembly of the F1F0 peripheral stalk; co-purifies with Aim43p, ATP synthase subunits, and cytochrome bc1 complex assembly factors; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect mitochondrion organization, protein complex biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-3] E 8 YIR025W MND2 - Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase mitotic cell cycle, meiotic cell cycle, organelle fission, regulation of organelle organization, proteolysis involved in cellular protein catabolic process, chromosome segregation, protein modification by small protein conjugation or removal, DNA recombination, regulation of cell cycle molecular_function nucleus BY4742 [00-3] E 9 YIR026C YVH1 tyrosine protein phosphatase YVH1 Dual specificity protein phosphatase; regulates growth, sporulation, and glycogen accumulation in a cAMP-dependent protein kinase cascade dependent manner; mutants are defective in 60S ribosome assembly; positively regulates pre-autophagosomal structure (PAS) formation upon nitrogen starvation or rapamycin treatment vacuole organization, meiotic cell cycle, organelle fission, sporulation, protein dephosphorylation, regulation of organelle organization, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly, cell wall organization or biogenesis, signaling phosphatase activity, hydrolase activity nucleus, cytoplasm BY4742 [00-3] E 10 YIR027C DAL1 allantoinase Allantoinase; converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression other hydrolase activity cellular_component BY4742 [00-3] E 11 YIR028W DAL4 allantoin permease Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation other transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-3] E 12 YIR029W DAL2 allantoicase|ALC1 Allantoicase; converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation other hydrolase activity cellular_component BY4742 [00-3] F 1 - cellular_component cellular_component cellular_component - - - - [00-3] F 2 YIR031C DAL7 malate synthase DAL7|MSL2|MLS2 Malate synthase; can accept butyryl-CoA as acyl-CoA donor in addition to traditional substrate acetyl-CoA; recycles glyoxylate generated during allantoin degradation; SWAT-GFP and mCherry fusion proteins localize to the cytosol; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation other transferase activity cytoplasm BY4742 F 3 - cytoplasm cytoplasm cytoplasm - - - - [00-3] F 4 YIR033W MGA2 - ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; MGA2 has a paralog, SPT23, that arose from the whole genome duplication response to chemical, chromatin organization, monocarboxylic acid metabolic process, lipid metabolic process, transcription from RNA polymerase II promoter molecular_function nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-3] F 5 YIR034C LYS1 saccharopine dehydrogenase (NAD+, L-lysine-forming) Saccharopine dehydrogenase (NAD+, L-lysine-forming); catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity cellular amino acid metabolic process RNA binding, mRNA binding, oxidoreductase activity cytoplasm BY4742 [00-3] F 6 YIR035C - sepiapterin reductase family protein Putative cytoplasmic short-chain dehydrogenase/reductase biological_process molecular_function membrane, cytoplasm BY4742 [00-3] F 7 YIR036C IRC24 sepiapterin reductase family protein IRC24 Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci biological_process oxidoreductase activity membrane, cytoplasm BY4742 [00-3] F 8 YIR037W HYR1 peroxiredoxin HYR1|ORP1|GPX3 Thiol peroxidase; functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor; HYR1 has a paralog, GPX1, that arose from the whole genome duplication response to chemical, response to oxidative stress oxidoreductase activity peroxisome, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-3] F 9 YIR038C GTT1 bifunctional glutathione transferase/peroxidase ER associated glutathione S-transferase; capable of homodimerization; glutathione transferase for Yvc1p vacuolar cation channel; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p other transferase activity, oxidoreductase activity endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [00-3] F 10 YIR039C YPS6 aspartyl protease Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance cell wall organization or biogenesis peptidase activity, hydrolase activity cell wall BY4742 [00-3] F 11 YIR042C - - Putative protein of unknown function; YIR042C is a non-essential gene biological_process molecular_function cellular_component BY4742 F 12 - cellular_component cellular_component cellular_component - - - - G 1 - - - - - - - - [00-3] G 2 YKL033W-A - putative hydrolase Putative protein of unknown function; similar to uncharacterized proteins from other fungi biological_process molecular_function cellular_component BY4742 G 3 - cellular_component cellular_component cellular_component - - - - [00-3] G 4 YKL162C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-3] G 5 YKR035W-A DID2 VPL30|VPS46|CHM1|FTI1 Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors protein targeting, endosomal transport molecular_function cytoplasmic vesicle, endomembrane system, cytoplasm BY4742 [00-3] G 6 YKR066C CCP1 cytochrome-c peroxidase Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress response to chemical, response to oxidative stress oxidoreductase activity mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-3] G 7 YKR067W GPT2 bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase GPT2|GAT1 Glycerol-3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen lipid metabolic process transferase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 G 8 - membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm - - - - [00-3] G 9 YKR069W MET1 uroporphyrinogen-III C-methyltransferase|MET20 S-adenosyl-L-methionine uroporphyrinogen III transmethylase; involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis cofactor metabolic process transferase activity, methyltransferase activity nucleus BY4742 [00-3] G 10 YKR070W - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 G 11 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-3] G 12 YKR072C SIS2 phosphopantothenoylcysteine decarboxylase complex subunit SIS2|HAL3 Negative regulatory subunit of protein phosphatase 1 (Ppz1p); involved in coenzyme A biosynthesis; subunit of phosphopantothenoylcysteine decarboxylase (PPCDC: Cab3p, Sis2p, Vhs3p) complex and the CoA-Synthesizing Protein Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p); SIS2 has a paralog, VHS3, that arose from the whole genome duplication mitotic cell cycle, cofactor metabolic process, nucleobase-containing small molecule metabolic process, response to osmotic stress, regulation of cell cycle enzyme regulator activity, lyase activity nucleus, cytoplasm BY4742 [00-3] H 1 YKR073C - - Putative protein of unknown function; conserved across S. cerevisiae strains biological_process molecular_function cellular_component BY4742 [00-3] H 2 YKR074W AIM29 - Protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cytoplasm BY4742 [00-3] H 3 YKR075C - - Protein of unknown function; similar to Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; YKR075C has a paralog, YOR062C, that arose from the whole genome duplication biological_process molecular_function nucleus, cytoplasm BY4742 [00-3] H 4 YKR076W ECM4 S-glutathionyl-(chloro)hydroquinone reductase|GTO2 S-glutathionyl-(chloro)hydroquinone reductase (GS-HQR); glutathione transferase involved in cell-surface biosynthesis and architecture; catalyzes glutathione (GSH)-dependent reduction of GS-trichloro-p-hydroquinone to trichloro-p-hydroquinone; expression upregulated upon exposure to genotoxic agents, such as methyl methanesulfonate, cisplatin and bleomycin; not an essential gene; similar to YGR154C; green fluorescent protein (GFP)-fusion protein localizes to cytoplasm other transferase activity cytoplasm BY4742 [00-3] H 5 YKR077W MSA2 - Putative transcriptional activator; interacts with G1-specific transcription factor MBF and G1-specific promoters; MSA2 has a paralog, MSA1, that arose from the whole genome duplication mitotic cell cycle molecular_function nucleus, cytoplasm BY4742 [00-3] H 6 YKR078W - - Cytoplasmic protein of unknown function; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P); YKR078W has a paralog, VPS5, that arose from the whole genome duplication biological_process ion binding, lipid binding cytoplasm BY4742 H 7 - cytoplasm cytoplasm cytoplasm - - - - [00-3] H 8 YKR080W MTD1 methylenetetrahydrofolate dehydrogenase (NAD(+)) NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase; plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity cytoplasm BY4742 H 9 - cytoplasm cytoplasm cytoplasm - - - - [00-3] H 10 YKR082W NUP133 RAT3 Subunit of Nup84p subcomplex of nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis; is involved in establishment of a normal nucleocytoplasmic concentration gradient of GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at nuclear periphery, including double-strand break repair, transcription and chromatin silencing; relocalizes to cytosol in response to hypoxia; homolog of human NUP133 response to heat, DNA repair, protein targeting, chromatin organization, cellular response to DNA damage stimulus, nucleobase-containing compound transport, transcription from RNA polymerase II promoter, nuclear transport, nucleus organization structural molecule activity nucleus, endomembrane system, membrane, cytoplasm BY4742 H 11 - nucleus, endomembrane system, membrane, cytoplasm nucleus, endomembrane system, membrane, cytoplasm nucleus, endomembrane system, membrane, cytoplasm - - - - [00-3] H 12 YKR084C HBS1 ribosome dissociation factor GTPase HBS1 GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled, particularly in 3' UTRs; genetically implicated in mRNA no-go decay; HBS1 has a paralog, SKI7, that arose from the whole genome duplication RNA catabolic process, regulation of translation GTPase activity, hydrolase activity cytoplasm BY4742 [00-4] A 1 - cytoplasm cytoplasm cytoplasm - - - - [00-4] A 2 - - - - - - - - [00-4] A 3 YKR087C OMA1 metalloendopeptidase Metalloendopeptidase of the mitochondrial inner membrane; important for adaptive responses to various homeostatic insults, preservation of normal mitochondrial function under damage-eliciting conditions, and stability of respiratory supercomplexes; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; zebrafish ortholog has a role in organ development and mouse ortholog is also required for respiratory supercomplex stability mitochondrion organization, proteolysis involved in cellular protein catabolic process, signaling peptidase activity, hydrolase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-4] A 4 YKR088C TVP38 - Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern mitotic cell cycle, organelle fission, cytoskeleton organization, chromosome segregation molecular_function membrane, endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-4] A 5 YKR089C TGL4 STC1 Multifunctional lipase/hydrolase/phospholipase; triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p; TGL4 has a paralog, TGL5, that arose from the whole genome duplication lipid metabolic process, cell budding transferase activity, hydrolase activity other BY4742 [00-4] A 6 YKR090W PXL1 - Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress signaling enzyme regulator activity cellular bud, site of polarized growth, cytoplasm BY4742 [00-4] A 7 YKR091W SRL3 DNA-binding protein SRL3|WHI7 GTB motif (G1/S transcription factor binding) containing protein; binds SBF-regulated promoters in hydroxyurea-treated cells; when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate; SRL3 has a paralog, WHI5, that arose from the whole genome duplication other DNA binding cytoplasm BY4742 [00-4] A 8 YKR092C SRP40 - Nucleolar serine-rich protein; role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 nuclear transport molecular_function nucleus, nucleolus BY4742 [00-4] A 9 YKR093W PTR2 - Integral membrane peptide transporter; mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p other other membrane, vacuole, plasma membrane, cytoplasm BY4742 [00-4] A 10 - membrane, vacuole, plasma membrane, cytoplasm membrane, vacuole, plasma membrane, cytoplasm membrane, vacuole, plasma membrane, cytoplasm - - - - [00-4] A 11 - - - - - - - - [00-4] A 12 - - - - - - - - [00-4] B 1 YKR097W PCK1 phosphoenolpyruvate carboxykinase PCK1|PPC1|JPM2 Phosphoenolpyruvate carboxykinase; key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol carbohydrate metabolic process lyase activity cytoplasm BY4742 [00-4] B 2 YKR098C UBP11 ubiquitin-specific protease UBP11 Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; UBP11 has a paralog, UBP7, that arose from the whole genome duplication biological_process peptidase activity, hydrolase activity cellular_component BY4742 [00-4] B 3 YKR099W BAS1 - Myb-related transcription factor; involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding nucleus BY4742 [00-4] B 4 YKR100C SKG1 YKR099C-A Transmembrane protein with a role in cell wall polymer composition; localizes on inner surface of plasma membrane at bud and in daughter cell; SKG1 has a paralog, AIM20, that arose from the whole genome duplication cell wall organization or biogenesis molecular_function cellular bud, site of polarized growth BY4742 [00-4] B 5 YKR101W SIR1 - Protein involved in silencing at mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin chromatin organization chromatin binding, DNA binding nucleus BY4742 [00-4] B 6 - nucleus nucleus nucleus - - - - [00-4] B 7 YKR103W NFT1 putative multidrug transporter NFT1 Putative transporter of the MRP subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds; MRP stands for multidrug resistance-associated protein other ATPase activity, hydrolase activity, transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-4] B 8 YKR104W - NFT1 Putative transporter of the MRP subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds; MRP stands for multidrug resistance-associated protein other ATPase activity, hydrolase activity, transmembrane transporter activity membrane BY4742 [00-4] B 9 YKR105C VBA5 basic amino acid transporter Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; VBA5 has a paralog, VBA3, that arose from a segmental duplication ion transport, amino acid transport molecular_function membrane, plasma membrane BY4742 [00-4] B 10 YLL018C-A COX19 - Protein required for cytochrome c oxidase assembly; located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs mitochondrion organization, ion transport, protein complex biogenesis ion binding mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-4] B 11 YLR262C-A TMA7 RBF7 Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress cytoplasmic translation molecular_function nucleus, cytoplasm BY4742 [00-4] B 12 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-4] C 1 YLR422W DCK1 - Dock family protein (Dedicator Of CytoKinesis), homolog of human DOCK1; upstream component for regulation through the small GTPase Rho5p; may form a complex with Lmo1p that acts as a GEF for Rho5p; interacts with Ino4p; cytoplasmic protein that relocates to mitochondria under oxidative stress; implicated in mitophagy; not an essential protein; DOCK proteins act as guanine nucleotide exchange factors mitochondrion organization, signaling molecular_function membrane, mitochondrion, plasma membrane, cytoplasm BY4742 [00-4] C 2 YLR423C ATG17 protein kinase regulatory subunit ATG17|APG17 Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells vacuole organization, protein phosphorylation, mitochondrion organization, organelle assembly, regulation of protein modification process enzyme regulator activity, structural molecule activity nucleus, cytoplasm BY4742 [00-4] C 3 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-4] C 4 YLR425W TUS1 Rho family guanine nucleotide exchange factor TUS1|SOP10 Guanine nucleotide exchange factor (GEF) that modulates Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate cell wall organization or biogenesis, signaling guanyl-nucleotide exchange factor activity cellular bud, site of polarized growth BY4742 [00-4] C 5 YLR426W TDA5 - Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele response to chemical molecular_function membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [00-4] C 6 YLR427W MAG2 - Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 biological_process molecular_function cytoplasm BY4742 [00-4] C 7 YLR428C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 biological_process molecular_function cellular_component BY4742 [00-4] C 8 YLR429W CRN1 - Coronin; cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly cytoskeleton organization, regulation of organelle organization, protein complex biogenesis protein binding, bridging, cytoskeletal protein binding cell cortex, cytoskeleton, cytoplasm BY4742 [00-4] C 9 - cell cortex, cytoskeleton, cytoplasm cell cortex, cytoskeleton, cytoplasm cell cortex, cytoskeleton, cytoplasm - - - - [00-4] C 10 YLR431C ATG23 CVT23 Peripheral membrane protein required for autophagy and CVT; required for cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p vacuole organization, protein targeting, organelle assembly molecular_function membrane, cytoplasm BY4742 [00-4] C 11 YLR432W IMD3 IMP dehydrogenase IMD3 Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in the de novo synthesis of GTP; member of a four-gene family in S. cerevisiae, constitutively expressed; IMD3 has a paralog, IMD4, that arose from the whole genome duplication nucleobase-containing small molecule metabolic process RNA binding, mRNA binding, oxidoreductase activity membrane, plasma membrane, cytoplasm BY4742 [00-4] C 12 YLR433C CNA1 calcineurin catalytic subunit A|CMP1 Calcineurin A; one isoform (the other is Cmp2p) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1; regulates the function of Aly1p alpha-arrestin; CNA1 has a paralog, CMP2, that arose from the whole genome duplication response to chemical, cellular ion homeostasis, conjugation, cell wall organization or biogenesis, signaling phosphatase activity, hydrolase activity cytoplasm BY4742 [00-4] D 1 YLR434C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W biological_process molecular_function cellular_component BY4742 [00-4] D 2 YLR435W TSR2 - Protein with a potential role in pre-rRNA processing rRNA processing, ribosomal small subunit biogenesis molecular_function nucleus, cytoplasm BY4742 [00-4] D 3 YLR436C ECM30 - Protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm BY4742 [00-4] D 4 YLR437C DIF1 - Protein that regulates nuclear localization of Rnr2p and Rnr4p; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half shows similarity to S. pombe Spd1 protein; DIF1 has a paralog, SML1, that arose from the whole genome duplication biological_process molecular_function cytoplasm BY4742 [00-4] D 5 - cytoplasm cytoplasm cytoplasm - - - - [00-4] D 6 YLR438W CAR2 ornithine-oxo-acid transaminase|cargB L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress; human homolog OAT complements yeast null mutant cellular amino acid metabolic process transferase activity nucleus, cytoplasm BY4742 [00-4] D 7 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-4] D 8 - - - - - - - - [00-4] D 9 YLR441C RPS1A eS1|ribosomal 40S subunit protein S1A|S1e|rp10A|S1A|RP10A Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-4] D 10 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [00-4] D 11 YLR443W ECM7 ZRG15 Putative integral membrane protein with a role in calcium uptake; non-essential protein; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency ion transport molecular_function membrane, vacuole, cytoplasm BY4742 [00-4] D 12 YLR444C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-4] E 1 YLR445W GMC2 - Protein involved in meiotic crossing over; component of the Synaptonemal Complex (SC) along with Ecm11p; required for the efficient loading of the SC transverse filament protein, Zip1p; promotes SUMOylation of Ecm11p; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor peptidyl-amino acid modification, meiotic cell cycle, organelle fission, protein modification by small protein conjugation or removal, DNA recombination molecular_function nucleus, chromosome BY4742 [00-4] E 2 YLR446W - hexokinase Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene biological_process molecular_function cellular_component BY4742 [00-4] E 3 YLR447C VMA6 H(+)-transporting V0 sector ATPase subunit d Subunit d of the V0 integral membrane domain of V-ATPase; part of the electrogenic proton pump found in the endomembrane system; required for V1 domain assembly on the vacuolar membrane; the V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase) has five subunits cellular ion homeostasis ATPase activity, hydrolase activity, transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-4] E 4 YLR448W RPL6B eL6|ribosomal 60S subunit protein L6B|L6e|rp18|YL16|L6B|L17B Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly RNA binding, structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [00-4] E 5 YLR449W FPR4 peptidylprolyl isomerase FPR4 Peptidyl-prolyl cis-trans isomerase (PPIase); nuclear proline isomerase; affects expression of multiple genes via its role in nucleosome assembly; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; contains a nucleoplasmin-like fold and can form pentamers peptidyl-amino acid modification, protein alkylation, chromatin organization, histone modification, regulation of protein modification process isomerase activity nucleus, chromosome, nucleolus BY4742 [00-4] E 6 YLR450W HMG2 hydroxymethylglutaryl-CoA reductase (NADPH) HMG2 HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at nuclear periphery upon DNA replication stress; HMG2 has a paralog, HMG1, that arose from the whole genome duplication; human homolog HMGCR can complement yeast hmg2 mutant lipid metabolic process oxidoreductase activity nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-4] E 7 YLR452C SST2 GTPase-activating protein SST2 GTPase-activating protein for Gpa1p; regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family response to chemical, conjugation, signaling enzyme regulator activity membrane, plasma membrane BY4742 [00-4] E 8 YLR453C RIF2 - Protein that binds to the Rap1p C-terminus; acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation; RIF2 has a paralog, ORC4, that arose from the whole genome duplication telomere organization DNA binding nucleus, chromosome BY4742 [00-4] E 9 YLR454W FMP27 - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-4] E 10 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-4] E 11 YLR456W - pyridoxal 5'-phosphate synthase Protein of unknown function; predicted to encode a pyridoxal 5'-phosphate synthase based on sequence similarity but purified protein does not possess this activity, nor does it bind flavin mononucleotide (FMN); null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4742 [00-4] E 12 - cellular_component cellular_component cellular_component - - - - [00-4] F 1 - - - - - - - - [00-4] F 2 - - - - - - - - [00-4] F 3 YLR460C - - Member of the quinone oxidoreductase family; up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine biological_process molecular_function cellular_component BY4742 [00-4] F 4 YLR461W PAU4 seripauperin PAU4 Member of the seripauperin multigene family; encoded mainly in subtelomeric regions; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to just the vacuole; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme biological_process molecular_function vacuole, cytoplasm BY4742 [00-4] F 5 YML009C MRPL39 mitochondrial 54S ribosomal protein YmL39|YmL39 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-4] F 6 YML010C-B ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm - - - BY4742 [00-4] F 7 YML021C UNG1 uracil-DNA glycosylase Uracil-DNA glycosylase; required for repair of uracil in DNA formed by spontaneous cytosine deamination; efficiently excises uracil from single-stranded DNA in vivo; not required for strand-specific mismatch repair; cell-cycle regulated, expressed in late G1; localizes to mitochondria and nucleus DNA repair, cellular response to DNA damage stimulus hydrolase activity, hydrolase activity, acting on glycosyl bonds nucleus, mitochondrion, cytoplasm BY4742 [00-4] F 8 YML081C-A ATP18 F1F0 ATP synthase subunit i Subunit of the mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms mitochondrion organization, transmembrane transport, ion transport, protein complex biogenesis, nucleobase-containing small molecule metabolic process hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-4] F 9 YMR060C SAM37 SAM complex subunit SAM37|TOM37|PET3027|MAS37 Component of the Sorting and Assembly Machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability protein targeting, mitochondrion organization, transmembrane transport, ion transport, protein complex biogenesis, lipid transport molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-4] F 10 YMR158C-B mitochondrion, membrane, mitochondrial envelope, cytoplasm mitochondrion, membrane, mitochondrial envelope, cytoplasm mitochondrion, membrane, mitochondrial envelope, cytoplasm - - - BY4742 [00-4] F 11 YMR169C ALD3 aldehyde dehydrogenase (NAD(+)) ALD3 Cytoplasmic aldehyde dehydrogenase; involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose cellular amino acid metabolic process oxidoreductase activity cytoplasm BY4742 [00-4] F 12 YMR174C PAI3 IA3 Cytoplasmic proteinase A (Pep4p) inhibitor; dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact other enzyme regulator activity nucleus, cytoplasm BY4742 [00-4] G 1 YMR175W SIP18 - Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress; SIP18 has a paralog, GRE1, that arose from the whole genome duplication response to chemical ion binding, lipid binding cytoplasm BY4742 [00-4] G 2 YMR194C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-4] G 3 YMR326C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 biological_process molecular_function cellular_component BY4742 [00-4] G 4 YNR032C-A HUB1 ubiquitin-like protein HUB1 Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear response to chemical, RNA splicing, cell morphogenesis, conjugation, mRNA processing other other BY4742 [00-4] G 5 YNR050C LYS9 saccharopine dehydrogenase (NADP+, L-glutamate-forming)|LYS13 Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 cellular amino acid metabolic process oxidoreductase activity cytoplasm BY4742 [00-4] G 6 YNR051C BRE5 - Ubiquitin protease cofactor; forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Golgi vesicle transport, protein modification by small protein conjugation or removal, regulation of transport RNA binding, mRNA binding cytoplasm BY4742 [00-4] G 7 - cytoplasm cytoplasm cytoplasm - - - - [00-4] G 8 - - - - - - - - [00-4] G 9 - - - - - - - - [00-4] G 10 - - - - - - - - [00-4] G 11 YNR056C BIO5 - Putative transmembrane protein involved in the biotin biosynthesis; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis cofactor metabolic process, vitamin metabolic process, monocarboxylic acid metabolic process transmembrane transporter activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [00-4] G 12 YNR057C BIO4 dethiobiotin synthase Dethiobiotin synthetase; catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; BIO3 and BIO4 were acquired by horizontal gene transfer (HGT) from bacteria; expression appears to be repressed at low iron levels cofactor metabolic process, vitamin metabolic process, monocarboxylic acid metabolic process ligase activity cytoplasm BY4742 [00-4] H 1 YNR058W BIO3 adenosylmethionine-8-amino-7-oxononanoate transaminase 7,8-diamino-pelargonic acid aminotransferase (DAPA); catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; BIO3 and BIO4 were acquired by horizontal gene transfer (HGT) from bacteria cofactor metabolic process, vitamin metabolic process, monocarboxylic acid metabolic process transferase activity cytoplasm BY4742 [00-4] H 2 YNR059W MNT4 putative alpha-1,3-mannosyltransferase Putative alpha-1,3-mannosyltransferase; not required for protein O-glycosylation; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively protein glycosylation transferase activity, transferring glycosyl groups, transferase activity cellular_component BY4742 [00-4] H 3 YNR060W FRE4 ferric-chelate reductase Ferric reductase; reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels other oxidoreductase activity membrane, plasma membrane BY4742 [00-4] H 4 YNR061C - - Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-4] H 5 YNR062C - - Putative membrane protein of unknown function biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-4] H 6 YNR063W - - Putative zinc-cluster protein of unknown function biological_process DNA binding cellular_component BY4742 [00-4] H 7 YNR064C - epoxide hydrolase Epoxide hydrolase; member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides response to chemical hydrolase activity cellular_component BY4742 [00-4] H 8 YNR065C - YSN1 Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-4] H 9 YNR066C - - Putative membrane-localized protein of unknown function biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-4] H 10 YNR067C DSE4 endo-1,3(4)-beta-glucanase|ENG1 Daughter cell-specific secreted protein with similarity to glucanases; degrades cell wall from the daughter side causing daughter to separate from mother other hydrolase activity, hydrolase activity, acting on glycosyl bonds cellular bud, cell wall, extracellular region, site of polarized growth BY4742 [00-4] H 11 YNR068C - BUL3 Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form biological_process molecular_function cellular_component BY4742 [00-4] H 12 - cellular_component cellular_component cellular_component - - - - [00-5] A 1 - - - - - - - - [00-5] A 2 - - - - - - - - [00-5] A 3 YER101C AST2 - Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication protein targeting molecular_function cytoplasm BY4742 [00-5] A 4 - cytoplasm cytoplasm cytoplasm - - - - [00-5] A 5 YER103W SSA4 Hsp70 family chaperone SSA4|YG107 Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication response to heat, protein targeting, transmembrane transport, protein folding hydrolase activity, unfolded protein binding, ATPase activity nucleus, cytoplasm BY4742 [00-5] A 6 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-5] A 7 - - - - - - - - [00-5] A 8 YER106W MAM1 - Monopolin; meiosis-specific kinetochore-associated protein involved in monopolar attachment of sister kinetochores to the meiotic spindle; subunit of monopolin, a complex that prevents biorientation of sister kinetochores to ensure homolog biorientation during meiosis I; regulates the conformation, enzyme kinetics and substrate specificity of the Dsn1p kinase, Hrr1p; expressed only during the first meiotic division meiotic cell cycle, protein phosphorylation, organelle fission, chromosome segregation, regulation of protein modification process molecular_function nucleus, chromosome BY4742 [00-5] A 9 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [00-5] A 10 YER108C - - - - - - BY4742 [00-5] A 11 YER109C - - - - - - BY4742 [00-5] A 12 YER110C KAP123 YRB4 Karyopherin beta; mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 protein targeting, pseudohyphal growth, nuclear transport protein transporter activity nucleus, endomembrane system, membrane, cytoplasm BY4742 [00-5] B 1 YER111C SWI4 SBF complex DNA-binding subunit SWI4|ART1 DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p response to heat, mitotic cell cycle, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, chromosome BY4742 [00-5] B 2 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [00-5] B 3 YER113C TMN3 - Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation invasive growth in response to glucose limitation, cellular ion homeostasis, pseudohyphal growth molecular_function cytoplasmic vesicle, endomembrane system, membrane, Golgi apparatus, cytoplasm BY4742 [00-5] B 4 YER114C BOI2 BEB1 Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication cell budding, signaling ion binding, lipid binding cellular bud, site of polarized growth BY4742 [00-5] B 5 YER115C SPR6 - Protein of unknown function; expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation meiotic cell cycle, sporulation molecular_function mitochondrion, cytoplasm BY4742 [00-5] B 6 YER116C SLX8 SUMO-targeted ubiquitin ligase complex subunit SLX8 Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; role in proteolysis of spindle positioning protein Kar9, DNA repair proteins Rad52p and Rad57p; stimulated by SUMO-modified substrates; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress; required for maintenance of genome integrity like human ortholog RNF peptidyl-amino acid modification, telomere organization, cellular response to DNA damage stimulus, protein modification by small protein conjugation or removal transferase activity nucleus, chromosome, cytoplasm BY4742 [00-5] B 7 YER117W RPL23B uL14|ribosomal 60S subunit protein L23B|L14|YL32|L23B|L17aB Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome nucleus, ribosome, cytoplasm BY4742 [00-5] B 8 YER118C SHO1 osmosensor SHO1|SSU81 Transmembrane osmosensor for filamentous growth and HOG pathways; involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol (HOG) response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p response to heat, signaling, response to osmotic stress enzyme binding, structural molecule activity, signal transducer activity endomembrane system, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [00-5] B 9 YER119C AVT6 aspartate/glutamate transporter Vacuolar aspartate and glutamate exporter; member of a family of seven genes (AVT1-7) related to vesicular GABA-glycine transporters; involved in compartmentalizing acidic amino acids in response to nitrogen starvation; AVT6 has a paralog, AVT5, that arose from the whole genome duplication transmembrane transport, ion transport, amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-5] B 10 YER119C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 biological_process molecular_function cellular_component BY4742 [00-5] B 11 YER120W SCS2 phosphatidylinositol-binding protein SCS2 Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to substrate PI4P in the PM; interacts with FFAT motifs in Opi1p, Swh1p, Osh2p, and Osh3p; involved in telomeric silencing; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication chromatin organization, regulation of transport, lipid transport, lipid metabolic process, protein targeting, organelle inheritance, nuclear transport ion binding, lipid binding nucleus, plasma membrane, endomembrane system, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm BY4742 [00-5] B 12 YER121W - - Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy biological_process molecular_function cellular_component BY4742 [00-5] C 1 YER122C GLO3 ADP-ribosylation factor GTPase-activating protein ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; shares functional similarity with Gcs1p vesicle organization, Golgi vesicle transport enzyme regulator activity cytoplasmic vesicle, endomembrane system, membrane, Golgi apparatus, cytoplasm BY4742 [00-5] C 2 YER123W YCK3 casein kinase YCK3|CKI3 Palmitoylated vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway protein phosphorylation kinase activity, transferase activity nucleus, vacuole, membrane, cytoplasm, plasma membrane BY4742 [00-5] C 3 YER124C DSE1 - Daughter cell-specific protein; may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress response to chemical, invasive growth in response to glucose limitation, conjugation, signaling molecular_function cellular bud, site of polarized growth BY4742 [00-5] C 4 - cellular bud, site of polarized growth cellular bud, site of polarized growth cellular bud, site of polarized growth - - - - [00-5] C 5 - - - - - - - - [00-5] C 6 - - - - - - - - [00-5] C 7 YER128W VFA1 - Protein that interacts with Vps4p and has a role in vacuolar sorting; stimulates the ATPase activity of Vps4; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology other enzyme regulator activity cytoplasmic vesicle, endomembrane system, cytoplasm BY4742 [00-5] C 8 YER129W SAK1 serine/threonine protein kinase SAK1|PAK1 Upstream serine/threonine kinase for the SNF1 complex; plays a role in pseudohyphal groth; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; SAK1 has a paralog, TOS3, that arose from the whole genome duplication peptidyl-amino acid modification, carbohydrate metabolic process, protein phosphorylation, DNA replication, pseudohyphal growth kinase activity, transferase activity cytoplasm BY4742 [00-5] C 9 YER130C COM2 - Transcription factor that binds IME1 Upstream Activation Signal (UAS)ru; COM2 transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; may bind the IME1 promoter under all growth conditions to negatively regulate its transcription in the absence of a positive regulator that binds more effectively; repressor activity may depend on phosphorylation by PKA; C. albicans homolog (MNL1) plays a role in adaptation to stress biological_process DNA binding cellular_component BY4742 [00-5] C 10 YER131W RPS26B eS26|ribosomal 40S subunit protein S26B|S26e|S26B Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication; human homolog can partially complement an RPS26A, RPS26B double null mutant; mutations in the human gene are associated with Diamond-Blackfan anemia cytoplasmic translation, organelle assembly, nucleobase-containing compound transport, ribosome assembly, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [00-5] C 11 YER132C PMD1 - Protein with an N-terminal kelch-like domain; putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions; PMD1 has a paralog, MDS3, that arose from the whole genome duplication sporulation molecular_function cytoplasm BY4742 [00-5] C 12 - cytoplasm cytoplasm cytoplasm - - - - [00-5] D 1 YER134C - Mg-dependent acid phosphatase|MDP-1 Magnesium-dependent acid phosphatase; member of the haloacid dehalogenase superfamily; non-essential gene biological_process phosphatase activity, hydrolase activity nucleus, cytoplasm BY4742 [00-5] D 2 YER135C - - Putative protein of unknown function; conserved among S. cerevisiae strains; YER135C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-5] D 3 - cellular_component cellular_component cellular_component - - - - [00-5] D 4 YER137C - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-5] D 5 YER139C RTR1 RNA polymerase II subunit B1 CTD phosphatase RTR1 CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication protein dephosphorylation, transcription from RNA polymerase II promoter phosphatase activity, hydrolase activity nucleus, cytoplasm BY4742 [00-5] D 6 YER140W EMP65 - Integral membrane protein of the ER; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS3; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies protein folding molecular_function membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [00-5] D 7 YER141W COX15 - Protein required for the hydroxylation of heme O to form heme A; heme A is an essential prosthetic group for cytochrome c oxidase cofactor metabolic process transmembrane transporter activity, oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-5] D 8 YER142C MAG1 MMS5 3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress DNA repair, cellular response to DNA damage stimulus DNA binding, hydrolase activity, hydrolase activity, acting on glycosyl bonds nucleus BY4742 [00-5] D 9 YER143W DDI1 VSM1 DNA damage-inducible v-SNARE binding protein; role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains proteolysis involved in cellular protein catabolic process ubiquitin-like protein binding membrane, plasma membrane BY4742 [00-5] D 10 YER145C FTR1 high-affinity iron permease FTR1 High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress cellular ion homeostasis, transmembrane transport, ion transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-5] D 11 - membrane, plasma membrane membrane, plasma membrane membrane, plasma membrane - - - - [00-5] D 12 - - - - - - - - [00-5] E 1 - - - - - - - - [00-5] E 2 YER149C PEA2 PPF2|DFG9 Coiled-coil 12S polarisome subunit; required for polarity establishment, apical bud growth, shmoo formation, filamentous differentiation; involved in Bni1p localization at sites of polarized growth, controlling polarized assembly of actin cables; role in apical growth affects diploid-specific bipolar bud site selection; retains Slt2p at bud tip to regulate ER inheritance; role in Ca2+ influx, cell fusion; S288C allele encoding Leu409 rather than Met linked with non-invasion mitotic cell cycle, response to chemical, cell morphogenesis, cell budding, cytoskeleton organization, regulation of organelle organization, cytokinesis, conjugation, pseudohyphal growth molecular_function cell cortex, cellular bud, site of polarized growth, cytoplasm BY4742 [00-5] E 3 YER150W SPI1 - GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; SPI1 has a paralog, SED1, that arose from the whole genome duplication other molecular_function cell wall BY4742 [00-5] E 4 YER151C UBP3 mRNA-binding ubiquitin-specific protease UBP3|BLM3 Ubiquitin-specific protease involved in transport and osmotic response; negatively regulates Ras/PKA signaling; interacts with Bre5p to coregulate anterograde, retrograde transport between ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; role in ribophagy; cleaves ubiquitin fusions but not polyubiquitin; protein abundance increases in response to DNA replication stress regulation of transport, Golgi vesicle transport, protein modification by small protein conjugation or removal, response to osmotic stress peptidase activity, RNA binding, hydrolase activity, mRNA binding cytoplasm BY4742 [00-5] E 5 YER152C - 2-aminoadipate transaminase Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene biological_process transferase activity nucleus, cytoplasm BY4742 [00-5] E 6 YER153C PET122 - Mitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis RNA binding, translation factor activity, RNA binding mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-5] E 7 YER154W OXA1 membrane insertase OXA1|PET1402 Mitochondrial inner membrane insertase; mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane; also has a role in insertion of carrier proteins into the inner membrane; acts as a voltage-gated ion channel, activated by substrate peptides; interacts with mitochondrial ribosomes; conserved from bacteria to animals protein targeting, mitochondrion organization, transmembrane transport, protein complex biogenesis protein transporter activity, transmembrane transporter activity ribosome, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-5] E 8 YER155C BEM2 TSL1|SUP9|IPL2 Rho GTPase activating protein (RhoGAP); involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence; potential GAP for Rho4p cytoskeleton organization, signaling RNA binding, enzyme regulator activity, mRNA binding cell cortex, mitochondrion, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane BY4742 [00-5] E 9 YER156C - - Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 biological_process molecular_function nucleus, cytoplasm BY4742 [00-5] E 10 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-5] E 11 YER158C - - Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4742 [00-5] E 12 - cellular_component cellular_component cellular_component - - - - [00-5] F 1 YER161C SPT2 EXA1|SIN1 Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components; relocalizes to the cytosol in response to hypoxia; similar to mammalian HMG1 proteins chromatin organization, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation DNA binding nucleus, cytoplasm BY4742 [00-5] F 2 YER162C RAD4 - Protein that recognizes and binds damaged DNA (with Rad23p) during NER; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins; Rad4p-Rad23p heterodimer binds to promoters of DNA damage response genes to repress their transcription in the absence of DNA damage; NER stands for nucleotide excision repair DNA repair, cellular response to DNA damage stimulus, transcription from RNA polymerase II promoter, proteolysis involved in cellular protein catabolic process DNA binding nucleus, cytoplasm BY4742 [00-5] F 3 YER163C GCG1 gamma-glutamylcyclotransferase Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle other transferase activity nucleus, cytoplasm BY4742 [00-5] F 4 YER164W CHD1 chromatin-remodeling ATPase CHD1 Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes transcription from RNA polymerase I promoter, peptidyl-amino acid modification, protein acylation, chromatin organization, DNA replication, DNA-templated transcription, termination, DNA-templated transcription, initiation, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation, protein complex biogenesis, histone modification, regulation of protein modification process, protein modification by small protein conjugation or removal, regulation of DNA metabolic process chromatin binding, hydrolase activity, histone binding, DNA binding, ATPase activity nucleus, chromosome, mitochondrion, nucleolus, cytoplasm BY4742 [00-5] F 5 - nucleus, chromosome, mitochondrion, nucleolus, cytoplasm nucleus, chromosome, mitochondrion, nucleolus, cytoplasm nucleus, chromosome, mitochondrion, nucleolus, cytoplasm - - - - [00-5] F 6 YER166W DNF1 aminophospholipid-translocating P4-type ATPase DNF1 Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF1 has a paralog, DNF2, that arose from the whole genome duplication response to chemical, ion transport, lipid transport, conjugation, endocytosis hydrolase activity, transmembrane transporter activity, ATPase activity endomembrane system, mitochondrion, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [00-5] F 7 YER167W BCK2 CTR7 Serine/threonine-rich protein involved in PKC1 signaling pathway; protein kinase C (PKC1) signaling pathway controls cell integrity; overproduction suppresses pkc1 mutations mitotic cell cycle, regulation of cell cycle molecular_function nucleus, cytoplasm BY4742 [00-5] F 8 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-5] F 9 YER169W RPH1 KDM4 JmjC domain-containing histone demethylase; targets tri- and dimethylated H3K36; associates with actively transcribed regions and promotes elongation; repressor of autophagy-related genes in nutrient-replete conditions; damage-responsive repressor of PHR1; phosphorylated by the Rad53p-dependent DNA damage checkpoint pathway and by a Rim1p-mediated event during starvation; target of stress-induced hormesis; RPH1 has a paralog, GIS1, that arose from the whole genome duplication chromatin organization, transcription from RNA polymerase II promoter, histone modification, DNA-templated transcription, elongation nucleic acid binding transcription factor activity, DNA binding, oxidoreductase activity nucleus BY4742 [00-5] F 10 YER170W ADK2 adenylate kinase ADK2|PAK3|AKY3 Mitochondrial adenylate kinase; catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background nucleobase-containing small molecule metabolic process kinase activity, transferase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-5] F 11 - membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm - - - - [00-5] F 12 - - - - - - - - [00-5] G 1 YER173W RAD24 RS1 Checkpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein meiotic cell cycle, DNA repair, organelle fission, cellular response to DNA damage stimulus, DNA recombination, regulation of cell cycle hydrolase activity, DNA binding, ATPase activity nucleus, chromosome BY4742 [00-5] G 2 YER174C GRX4 monothiol glutaredoxin GRX4 Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; with Grx3p, binds to Aft1p in iron-replete conditions, promoting its dissociation from promoters; mutant has increased aneuploidy tolerance; transcription regulated by Yap5p; GRX4 has a paralog, GRX3, that arose from the whole genome duplication response to chemical, chromatin organization, cytoskeleton organization, response to oxidative stress, histone modification, cellular ion homeostasis, regulation of protein modification process oxidoreductase activity, transcription factor binding nucleus BY4742 [00-5] G 3 YER175C TMT1 TAM1 Trans-aconitate methyltransferase; cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle biological_process transferase activity, methyltransferase activity cytoplasm BY4742 [00-5] G 4 YER176W ECM32 MTT1|HEL1 DNA dependent ATPase/DNA helicase; helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes regulation of translation RNA binding, mRNA binding, helicase activity, hydrolase activity cytoplasm BY4742 [00-5] G 5 YER177W BMH1 14-3-3 family protein BMH1|APR6 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling, aggresome formation, spindle position checkpoint; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; antiapoptotic gene similar to human 14-3-3; BMH1 has a paralog, BMH2, that arose from whole genome duplication mitotic cell cycle, meiotic cell cycle, organelle fission, generation of precursor metabolites and energy, cell wall organization or biogenesis, transcription from RNA polymerase II promoter, carbohydrate metabolic process, sporulation, regulation of organelle organization, cellular response to DNA damage stimulus, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, pseudohyphal growth, signaling, regulation of cell cycle ion binding, DNA binding, transcription factor binding nucleus, plasma membrane, membrane, cytoplasm BY4742 [00-5] G 6 YER178W PDA1 pyruvate dehydrogenase (acetyl-transferring) subunit E1 alpha E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex; catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes cofactor metabolic process, pseudohyphal growth, monocarboxylic acid metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4742 [00-5] G 7 YER179W DMC1 recombinase DMC1|ISC2 Meiosis-specific recombinase required for double-strand break repair; also required for pairing between homologous chromosomes; required for the normal morphogenesis of synaptonemal complex; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p meiotic cell cycle, organelle fission, DNA recombination, chromosome segregation DNA binding nucleus, chromosome BY4742 [00-5] G 8 YER180C ISC10 - Protein required for sporulation; transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells sporulation molecular_function cellular_component BY4742 [00-5] G 9 YER181C - - Mitochondrial protein of unknown function; conserved among S. cerevisiae strains; extensively overlaps a Ty1 LTR; YER181C is not an essential gene biological_process molecular_function membrane, mitochondrion, cytoplasm BY4742 [00-5] G 10 YER182W FMP10 - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-5] G 11 YER183C FAU1 5-formyltetrahydrofolate cyclo-ligase 5,10-methenyltetrahydrofolate synthetase; involved in folic acid biosynthesis cofactor metabolic process ligase activity mitochondrion, cytoplasm BY4742 [00-5] G 12 YER184C TOG1 - Transcriptional activator of oleate genes; regulates genes involved in fatty acid utilization; zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [00-5] H 1 YER185W PUG1 - Plasma membrane protein involved in protoprophyrin and heme transport; roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins other molecular_function membrane, plasma membrane BY4742 [00-5] H 2 YER186C - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-5] H 3 YER187W - - Putative protein of unknown function; induced in respiratory-deficient cells biological_process molecular_function cellular_component BY4742 [00-5] H 4 - cellular_component cellular_component cellular_component - - - - [00-5] H 5 YMR052C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane BY4742 [00-5] H 6 YMR052W FAR3 - Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress response to chemical, conjugation, signaling molecular_function endomembrane system, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4742 [00-5] H 7 YMR053C STB2 - Protein that interacts with Sin3p in a two-hybrid assay; part of a large protein complex with Sin3p and Stb1p; STB2 has a paralog, STB6, that arose from the whole genome duplication biological_process molecular_function nucleus, chromosome BY4742 [00-5] H 8 YMR054W STV1 H(+)-transporting V0 sector ATPase subunit a Subunit a of the vacuolar-ATPase V0 domain; one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole cellular ion homeostasis hydrolase activity, transmembrane transporter activity, ATPase activity cytoplasmic vesicle, endomembrane system, vacuole, membrane, Golgi apparatus, cytoplasm BY4742 [00-5] H 9 YMR055C BUB2 PAC7 Mitotic exit network regulator; forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage mitotic cell cycle, organelle fission, regulation of organelle organization, chromosome segregation, regulation of cell cycle enzyme regulator activity cytoskeleton, microtubule organizing center, cytoplasm BY4742 [00-5] H 10 YMR056C AAC1 ADP/ATP carrier protein AAC1 Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-5] H 11 YMR057C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 biological_process molecular_function cellular_component BY4742 [00-5] H 12 YMR058W FET3 ferroxidase FET3 Ferro-O2-oxidoreductase; multicopper oxidase that oxidizes ferrous (Fe2+) to ferric iron (Fe3+) for subsequent cellular uptake by transmembrane permease Ftr1p; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress response to chemical, transmembrane transport, ion transport, cellular ion homeostasis transmembrane transporter activity, oxidoreductase activity endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [00-6] A 1 - endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm, plasma membrane - - - - [00-6] A 2 - - - - - - - - [00-6] A 3 YMR062C ARG7 glutamate N-acetyltransferase|ECM40 Mitochondrial ornithine acetyltransferase; catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine cellular amino acid metabolic process transferase activity mitochondrion, cytoplasm BY4742 [00-6] A 4 YMR063W RIM9 - Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; meiotic cell cycle, sporulation molecular_function membrane, plasma membrane BY4742 [00-6] A 5 YMR064W AEP1 NCA1 Protein required for expression of the mitochondrial OLI1 gene; mitochondrial OLI1 gene encodes subunit 9 of F1-F0 ATP synthase regulation of translation other mitochondrion, cytoplasm BY4742 [00-6] A 6 YMR065W KAR5 FIG3 Protein required for nuclear membrane fusion during karyogamy; localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; similar to zebrafish Brambleberry protein; expression of the gene is regulated by pheromone organelle fusion, conjugation, nucleus organization molecular_function endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-6] A 7 YMR066W SOV1 - Mitochondrial protein of unknown function biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-6] A 8 YMR067C UBX4 CUI1 UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress response to chemical, sporulation, proteolysis involved in cellular protein catabolic process other nucleus, membrane, cytoplasm BY4742 [00-6] A 9 YMR068W AVO2 - Component of a complex containing the Tor2p kinase and other proteins; complex may have a role in regulation of cell growth cytoskeleton organization, cell wall organization or biogenesis, signaling molecular_function nucleus, cytoplasm BY4742 [00-6] A 10 YMR069W NAT4 NAA40 N alpha-acetyl-transferase; involved in acetylation of the N-terminal residues of histones H4 and H2A peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity nucleus, cytoplasm BY4742 [00-6] A 11 YMR070W MOT3 [MOT3]|[MOT3+]|ROX7 Transcriptional repressor, activator; role in cellular adjustment to osmotic stress including modulation of mating efficiency; involved in repression of subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of Tup1p-Cyc8p general repressor to promoters; relocalizes to cytosol under hypoxia; forms [MOT3+] prion under anaerobic conditions lipid metabolic process, transcription from RNA polymerase II promoter, response to osmotic stress nucleic acid binding transcription factor activity, DNA binding, transcription factor binding nucleus, cytoplasm BY4742 [00-6] A 12 YMR071C TVP18 - Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments; may have a role in intracellular sterol transport other molecular_function membrane, cytoplasmic vesicle, endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-6] B 1 YMR072W ABF2 DNA-binding protein ABF2|mtTFA|HM|p19 Mitochondrial DNA-binding protein; involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation; ABF2 has a paralog, IXR1, that arose from the whole genome duplication; human homolog TFAM can complement yeast abf2 mutant, rescuing the loss-of-mitochondrial DNA phenotype in a yeast abf2 strain organelle inheritance, mitochondrion organization DNA binding chromosome, mitochondrion, cytoplasm BY4742 [00-6] B 2 YMR073C IRC21 - Protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study response to chemical molecular_function cytoplasm BY4742 [00-6] B 3 - cytoplasm cytoplasm cytoplasm - - - - [00-6] B 4 YMR075C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W biological_process molecular_function cellular_component BY4742 [00-6] B 5 YMR075W RCO1 - Essential component of the Rpd3S histone deacetylase complex; interacts with Eaf3p chromatin organization, DNA replication, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, histone modification, DNA-templated transcription, elongation molecular_function nucleus, chromosome BY4742 [00-6] B 6 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [00-6] B 7 YMR077C VPS20 ESCRT-III subunit protein VPS20|VPL10|VPT20|CHM6 Myristoylated subunit of the ESCRT-III complex; the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes ion transport, vesicle organization, proteolysis involved in cellular protein catabolic process, nucleobase-containing compound transport, endosomal transport other cytoplasmic vesicle, endomembrane system, membrane, cytoplasm BY4742 [00-6] B 8 YMR078C CTF18 CHL12 Subunit of a complex with Ctf8p; shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint mitotic cell cycle, DNA repair, organelle fission, DNA replication, cellular response to DNA damage stimulus, chromosome segregation, DNA recombination molecular_function nucleus, chromosome, mitochondrion, cytoplasm BY4742 [00-6] B 9 - nucleus, chromosome, mitochondrion, cytoplasm nucleus, chromosome, mitochondrion, cytoplasm nucleus, chromosome, mitochondrion, cytoplasm - - - - [00-6] B 10 YMR080C NAM7 SUT2|ATP-dependent RNA helicase NAM7|SUP113|UPF1|MOF4|IFS2 ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; binds to the small ribosomal subunit via an interaction with Rps26; forms cytoplasmic foci upon DNA replication stress regulation of translation, chromatin organization, RNA catabolic process, protein modification by small protein conjugation or removal, DNA recombination RNA binding, hydrolase activity, ATPase activity, helicase activity, mRNA binding cytoplasm BY4742 [00-6] B 11 YMR081C ISF1 MBR3 Serine-rich, hydrophilic protein; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant; ISF1 has a paralog, MBR1, that arose from the whole genome duplication generation of precursor metabolites and energy, cellular respiration molecular_function cellular_component BY4742 [00-6] B 12 YMR082C - - Putative protein of unknown function; conserved among S. cerevisiae strains; YMR082C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-6] C 1 YMR083W ADH3 alcohol dehydrogenase ADH3 Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production cofactor metabolic process, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4742 [00-6] C 2 YMR084W - putative glutamine--fructose-6-phosphate transaminase Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 biological_process molecular_function cellular_component BY4742 [00-6] C 3 YMR085W - - Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 biological_process molecular_function cellular_component BY4742 [00-6] C 4 YMR086C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-6] C 5 YMR086W SEG1 - Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation, controls eisosome length and shape; diffusely distributed, forms heterogeneous patches at plasma membrane in small buds, also found in medium and large buds; expression repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes; SEG1 has a paralog, SEG2, that arose from the whole genome duplication other molecular_function ribosome, cytoplasm BY4742 [00-6] C 6 YMR087W - putative ADP-ribose 1''-phosphate phosphatase Putative ADP-ribose-1''-monophosphatase; converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain RNA splicing, tRNA processing phosphatase activity, hydrolase activity cellular_component BY4742 [00-6] C 7 YMR088C VBA1 - Permease of basic amino acids in the vacuolar membrane ion transport, amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-6] C 8 YMR089C YTA12 m-AAA protease subunit YTA12|RCA1 Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; overexpression of human AFG3L2 complements respiratory defect of yeast afg3 yta12 double null mutation, but overexpression of disease-associated AFG3L2 variants does not; expression of both human SPG7 (paraplegin) and AFG3L2 complements yeast yta12 afg3 double mutation protein targeting, mitochondrion organization, transmembrane transport, protein maturation, protein complex biogenesis peptidase activity, hydrolase activity, ion binding, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-6] C 9 YMR090W - - Putative protein of unknown function; similar to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability biological_process molecular_function cytoplasm BY4742 [00-6] C 10 YMR091C NPL6 RSC7 Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Ldb7p, and Htl1p to form a module important for a broad range of RSC functions chromatin organization, cellular response to DNA damage stimulus, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation ATPase activity, hydrolase activity nucleus BY4742 [00-6] C 11 YMR092C AIP1 - Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; inhibits elongation of aged ADP-actin filaments decorated with cofilin to maintain a high level of assembly-competent actin species; required to restrict cofilin localization to cortical patches; putative regulator of cytokinesis; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress cytoskeleton organization, regulation of organelle organization, cytokinesis, protein complex biogenesis, response to osmotic stress, regulation of cell cycle cytoskeletal protein binding nucleus, cell cortex, cytoskeleton, site of polarized growth, cytoplasm BY4742 [00-6] C 12 - nucleus, cell cortex, cytoskeleton, site of polarized growth, cytoplasm nucleus, cell cortex, cytoskeleton, site of polarized growth, cytoplasm nucleus, cell cortex, cytoskeleton, site of polarized growth, cytoplasm - - - - [00-6] D 1 YNR070W PDR18 ATP-binding cassette multidrug transporter PDR18 Putative transporter of the ATP-binding cassette (ABC) family; role in plasma membrane sterol incorporation; implicated in pleiotropic drug resistance; provides resistance to ethanol stress and contributes to a decreased intracellular accumulation of ethanol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies response to chemical hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, cytoplasm, plasma membrane BY4742 [00-6] D 2 YNR071C - aldose 1-epimerase superfamily protein Putative aldose 1-epimerase biological_process molecular_function cellular_component BY4742 [00-6] D 3 YNR072W HXT17 HLT4|hexose transporter HXT17 Putative transmembrane polyol transporter; supports growth on and uptake of mannitol and sorbitol with moderate affinity when overexpressed in a strain deleted for hexose family members; minor hexose transport activity when overexpressed in a similar strain; induced by raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose; HXT17 has a paralog, HXT13, that arose from a segmental duplication carbohydrate transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [00-6] D 4 YNR073C MAN2 putative mannitol dehydrogenase Mannitol dehydrogenase; MAN2 has a paralog, DSF1, that arose from a segmental duplication biological_process oxidoreductase activity cellular_component BY4742 [00-6] D 5 YNR074C AIF1 CPD1 Mitochondrial cell death effector; translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase response to chemical, response to oxidative stress oxidoreductase activity nucleus, mitochondrion, membrane, cytoplasm, plasma membrane BY4742 [00-6] D 6 YNR075W COS10 - Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins protein targeting, proteolysis involved in cellular protein catabolic process, endosomal transport, endocytosis molecular_function nucleus, cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-6] D 7 YOL013W-A - YOL013W-B Putative protein of unknown function; identified by SAGE biological_process molecular_function membrane BY4742 [00-6] D 8 YOL086C ADH1 alcohol dehydrogenase ADH1|ADC1 Alcohol dehydrogenase; fermentative isozyme active as homo- or heterotetramers; required for the reduction of acetaldehyde to ethanol, the last step in the glycolytic pathway; ADH1 has a paralog, ADH5, that arose from the whole genome duplication carbohydrate metabolic process, cofactor metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, cellular amino acid metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity membrane, cytoplasm, plasma membrane BY4742 - [00-6] D 9 YOL087C DUF1 - Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid biological_process ubiquitin-like protein binding cytoplasm BY4742 [00-6] D 10 YOL088C MPD2 protein disulfide isomerase MPD2 Member of the protein disulfide isomerase (PDI) family; exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p protein folding isomerase activity, oxidoreductase activity endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-6] D 11 YOL089C HAL9 - Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication transcription from RNA polymerase II promoter, response to osmotic stress DNA binding nucleus, mitochondrion, cytoplasm BY4742 [00-6] D 12 YOL090W MSH2 mismatch repair ATPase MSH2|PMS5 Protein that binds to DNA mismatches; forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP meiotic cell cycle, DNA repair, organelle fission, chromatin organization, DNA replication, cellular response to DNA damage stimulus, DNA recombination, regulation of DNA metabolic process hydrolase activity, DNA binding, ion binding, ATPase activity nucleus, chromosome BY4742 [00-6] E 1 YOL091W SPO21 MPC70 Component of the meiotic outer plaque of the spindle pole body; involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation; SPO21 has a paralog, YSW1, that arose from the whole genome duplication meiotic cell cycle, organelle fission, sporulation structural molecule activity cytoskeleton, microtubule organizing center BY4742 [00-6] E 2 YOL092W YPQ1 cationic amino acid transporter Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter; YPQ1 has a paralog, RTC2, that arose from the whole genome duplication transmembrane transport, ion transport, amino acid transport transmembrane transporter activity endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-6] E 3 YOL093W TRM10 tRNA (guanine(9)-N(1))-methyltransferase tRNA methyltransferase; methylates the N-1 position of guanine at position 9 in tRNAs; protein abundance increases in response to DNA replication stress; member of the SPOUT (SpoU-TrmD) methyltransferase family; human ortholog TRMT10A plays a role in the pathogenesis of microcephaly and early onset diabetes; an 18-mer originates from the TRM10 locus; genetic analysis shows the 18-mer is the translation regulator tRNA processing, RNA modification transferase activity, methyltransferase activity nucleus, cytoplasm BY4742 [00-6] E 4 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-6] E 5 YOL095C HMI1 ATP-dependent 3'-5' DNA helicase Mitochondrial inner membrane localized ATP-dependent DNA helicase; required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD mitochondrion organization helicase activity, ATPase activity, hydrolase activity mitochondrion, cytoplasm BY4742 [00-6] E 6 YOL096C COQ3 hexaprenyldihydroxybenzoate methyltransferase O-methyltransferase; catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein cofactor metabolic process transferase activity, methyltransferase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-6] E 7 - membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm - - - - [00-6] E 8 YOL098C SDD3 - Putative metalloprotease; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P biological_process molecular_function cytoplasm BY4742 [00-6] E 9 YOL099C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes biological_process molecular_function ribosome, cytoplasm BY4742 [00-6] E 10 YOL100W PKH2 serine/threonine protein kinase PKH2 Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh1p; PKH2 has a paralog, PKH1, that arose from the whole genome duplication peptidyl-amino acid modification, protein phosphorylation, organelle assembly, RNA catabolic process, endosomal transport, cell wall organization or biogenesis, signaling kinase activity, transferase activity nucleus, cell cortex, cytoplasm BY4742 [00-6] E 11 YOL101C IZH4 - Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress; IZH4 has a paralog, IZH1, that arose from the whole genome duplication cellular ion homeostasis molecular_function nucleus, membrane, vacuole, cytoplasm BY4742 [00-6] E 12 - nucleus, membrane, vacuole, cytoplasm nucleus, membrane, vacuole, cytoplasm nucleus, membrane, vacuole, cytoplasm - - - - [00-6] F 1 YOL103W ITR2 myo-inositol transporter ITR2|HRB612 Myo-inositol transporter; member of the sugar transporter superfamily; expressed constitutively; ITR2 has a paralog, ITR1, that arose from the whole genome duplication other transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [00-6] F 2 YOL104C NDJ1 TAM1 Protein that regulates meiotic SPB cohesion and telomere clustering; localizes to both spindle pole bodies (SPBs) and telomeres; required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement meiotic cell cycle, organelle fission, regulation of organelle organization, chromosome segregation, DNA recombination, regulation of DNA metabolic process, regulation of cell cycle DNA binding nucleus, cytoskeleton, microtubule organizing center, chromosome BY4742 [00-6] F 3 YOL105C WSC3 - Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity; involved in response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis; WSC3 has a paralog, WSC2, that arose from the whole genome duplication response to heat, signaling signal transducer activity site of polarized growth, vacuole, cytoplasm BY4742 [00-6] F 4 YOL106W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane BY4742 [00-6] F 5 YOL107W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein biological_process molecular_function cytoplasmic vesicle, endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-6] F 6 YOL108C INO4 - Transcription factor involved in phospholipid synthesis; required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain lipid metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [00-6] F 7 YOL109W ZEO1 - Peripheral membrane protein of the plasma membrane; interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria cell wall organization or biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, plasma membrane, cytoplasm BY4742 [00-6] F 8 YOL110W SHR5 ERF4 Palmitoyltransferase subunit; this complex adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization; Palmitoyltransferase is composed of Shr5p and Erf2 protein targeting, protein acylation, protein lipidation transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-6] F 9 YOL111C MDY2 GET5|TMA24 Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes response to chemical, protein targeting, cell morphogenesis, conjugation molecular_function nucleus, ribosome, cytoplasm BY4742 [00-6] F 10 YOL112W MSB4 Rab GTPase-activating protein MSB4 GTPase-activating protein of the Ras superfamily; acts primarily on Sec4p, localizes to the bud site and bud tip; msb3 msb4 double mutation causes defects in secretion and actin organization; similar to the TBC-domain Tre2 oncogene; MSB4 has a paralog, MSB3, that arose from the whole genome duplication; human homolog USP6NL can complement yeast msb3 msb4 double null mutant exocytosis, cytoskeleton organization enzyme regulator activity cellular bud, cell cortex, site of polarized growth, cytoplasm BY4742 [00-6] F 11 YOL113W SKM1 putative serine/threonine protein kinase SKM1 Member of the PAK family of serine/threonine protein kinases; similar to Ste20p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth; SKM1 has a paralog, CLA4, that arose from the whole genome duplication lipid transport, protein phosphorylation, regulation of transport, transcription from RNA polymerase II promoter kinase activity, transferase activity nucleus, membrane, plasma membrane BY4742 [00-6] F 12 YOL114C PTH4 - Protein similar to the human peptidyl-tRNA hydrolase gene ICT1; associates with mitochondrial large subunit; may function in translation termination; YOL114C is not an essential gene mitochondrial translation, mitochondrion organization hydrolase activity ribosome, mitochondrion, cytoplasm BY4742 [00-6] G 1 YOL115W PAP2 non-canonical poly(A) polymerase PAP2|TRF4 Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of TRAMP; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; required for mRNA surveillance and maintenance of genome integrity, serving as a link between RNA and DNA metabolism; overlapping but non-redundant functions with Trf5p; relocalizes to cytosol in response to hypoxia meiotic cell cycle, snoRNA processing, organelle fission, tRNA processing, DNA recombination, RNA modification, DNA repair, cellular response to DNA damage stimulus, RNA catabolic process, regulation of DNA metabolic process RNA binding, nucleotidyltransferase activity, hydrolase activity, transferase activity, lyase activity, mRNA binding, helicase activity, ATPase activity nucleus, nucleolus, cytoplasm BY4742 [00-6] G 2 YOL116W MSN1 PHD2|MSS10|HRB382|FUP1 Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress response to heat, carbohydrate metabolic process, response to chemical, invasive growth in response to glucose limitation, pseudohyphal growth, transcription from RNA polymerase II promoter, response to osmotic stress DNA binding nucleus, cytoplasm BY4742 [00-6] G 3 YOL117W RRI2 CSN10 Subunit of the COP9 signalosome (CSN) complex; this complex cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response response to chemical, protein modification by small protein conjugation or removal, conjugation, signaling molecular_function nucleus BY4742 [00-6] G 4 YOL118C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-6] G 5 YOL119C MCH4 - Protein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport other other membrane, vacuole, cytoplasm BY4742 [00-6] G 6 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [00-6] G 7 YOL121C RPS19A eS19|ribosomal 40S subunit protein S19A|S19e|rp55a|YS16A|S19A|S16aA Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication cytoplasmic translation, nucleobase-containing compound transport, ribosomal small subunit biogenesis, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [00-6] G 8 YOL122C SMF1 divalent metal ion transporter SMF1|SBS1 Divalent metal ion transporter; broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins cellular ion homeostasis, ion transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [00-6] G 9 - membrane, vacuole, plasma membrane, cytoplasm membrane, vacuole, plasma membrane, cytoplasm membrane, vacuole, plasma membrane, cytoplasm - - - - [00-6] G 10 YOL124C TRM11 tRNA (guanine-N2-)-methyltransferase Catalytic subunit of adoMet-dependent tRNA methyltransferase complex; required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain; another complex member is Trm112p tRNA processing, RNA modification RNA binding, transferase activity, methyltransferase activity cytoplasm BY4742 [00-6] G 11 - cytoplasm cytoplasm cytoplasm - - - - [00-6] G 12 YOL126C MDH2 malate dehydrogenase MDH2 Cytoplasmic malate dehydrogenase; one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 carbohydrate metabolic process, protein targeting, transmembrane transport, peroxisome organization oxidoreductase activity nucleus, cytoplasm BY4742 [00-6] H 1 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-6] H 2 YOL128C YGK3 serine/threonine protein kinase YGK3 Protein kinase related to mammalian GSK-3 glycogen synthase kinases; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation; YGK3 has a paralog, MCK1, that arose from the whole genome duplication response to heat, protein phosphorylation, response to osmotic stress kinase activity, transferase activity cellular_component BY4742 [00-6] H 3 YOL129W VPS68 - Vacuolar membrane protein of unknown function; involved in vacuolar protein sorting; also detected in the mitochondria protein targeting, endosomal transport molecular_function cytoplasmic vesicle, endomembrane system, vacuole, mitochondrion, membrane, cytoplasm BY4742 [00-6] H 4 - cytoplasmic vesicle, endomembrane system, vacuole, mitochondrion, membrane, cytoplasm cytoplasmic vesicle, endomembrane system, vacuole, mitochondrion, membrane, cytoplasm cytoplasmic vesicle, endomembrane system, vacuole, mitochondrion, membrane, cytoplasm - - - - [00-6] H 5 YOL131W - - Putative protein of unknown function; YOL131W has a paralog, STB1, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4742 [00-6] H 6 YOL132W GAS4 1,3-beta-glucanosyltransferase 1,3-beta-glucanosyltransferase; involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall meiotic cell cycle, sporulation, cell wall organization or biogenesis transferase activity vacuole, cell wall, membrane, cytoplasm BY4742 [00-6] H 7 - vacuole, cell wall, membrane, cytoplasm vacuole, cell wall, membrane, cytoplasm vacuole, cell wall, membrane, cytoplasm - - - - [00-6] H 8 - - - - - - - - [00-6] H 9 - - - - - - - - [00-6] H 10 YOL136C PFK27 6-phosphofructo-2-kinase|PFK-2 6-phosphofructo-2-kinase; catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process kinase activity, transferase activity cytoplasm BY4742 [00-6] H 11 YOL137W BSC6 - Protein of unknown function with 8 putative transmembrane segments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression biological_process molecular_function membrane, cytoplasmic vesicle, endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-6] H 12 YOL138C RTC1 SEA2 Subunit of SEACAT, a subcomplex of the SEA complex; Rtc1p, along with Mtc5p and Sea4p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; null suppresses cdc13-1 signaling molecular_function membrane, vacuole, ribosome, cytoplasm BY4742 [00-8] A 1 YBR232C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-8] A 2 - cellular_component cellular_component cellular_component - - - - [00-8] A 3 YDR424C DYN2 dynein light chain|SLC1 Cytoplasmic light chain dynein, microtubule motor protein; required for intracellular transport and cell division; involved in mitotic spindle positioning; forms complex with dynein intermediate chain Pac11p that promotes Dyn1p homodimerization, potentiates motor processivity; Dyn2p-Pac11p complex important for interaction of dynein motor complex with dynactin complex; acts as molecular glue to dimerize, stabilize Nup82-Nsp1-Nup159 complex module of cytoplasmic pore filaments mitotic cell cycle, cytoskeleton organization, protein complex biogenesis, nucleus organization hydrolase activity, ATPase activity peroxisome, nucleus, cytoskeleton, endomembrane system, cytoplasm BY4742 [00-8] A 4 YEL011W GLC3 1,4-alpha-glucan branching enzyme|GHA1 Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; glycogen accumulation defect of the null mutant is functionally complemented by human GBE1, which is associated with glycogen storage disease carbohydrate metabolic process, generation of precursor metabolites and energy transferase activity, transferring glycosyl groups, transferase activity cytoplasm BY4742 [00-8] A 5 - cytoplasm cytoplasm cytoplasm - - - - [00-8] A 6 YER064C VHR2 - Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress other molecular_function nucleus, cytoplasm BY4742 [00-8] A 7 YER077C MRX1 - Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-8] A 8 YER078C ICP55 aminopeptidase Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family protein maturation peptidase activity, hydrolase activity nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-8] A 9 YER088C DOT6 PBF2 Protein involved in rRNA and ribosome biogenesis; activated in stochastic pulses of nuclear localization; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; relative distribution to the nucleus increases upon DNA replication stress chromatin organization, transcription from RNA polymerase II promoter DNA binding nucleus, chromosome, cytoplasm BY4742 [00-8] A 10 - nucleus, chromosome, cytoplasm nucleus, chromosome, cytoplasm nucleus, chromosome, cytoplasm - - - - [00-8] A 11 YER090W TRP2 anthranilate synthase TRP2 Anthranilate synthase; catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p cellular amino acid metabolic process lyase activity cytoplasm BY4742 [00-8] A 12 YER091C MET6 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase Cobalamin-independent methionine synthase; involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs cellular amino acid metabolic process transferase activity, methyltransferase activity membrane, plasma membrane, cytoplasm BY4742 [00-8] B 1 YER092W IES5 - Non-essential INO80 chromatin remodeling complex subunit; deletion affects telomere maintenance via recombination telomere organization, DNA recombination molecular_function nucleus, chromosome BY4742 [00-8] B 2 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [00-8] B 3 YER093C-A AIM11 GEP8 Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; SWAT-GFP and mCherry fusion proteins localize to the mitochondria; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-8] B 4 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-8] B 5 YER095W RAD51 recombinase RAD51|MUT5 Strand exchange protein; forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein meiotic cell cycle, telomere organization, organelle fission, DNA recombination hydrolase activity, DNA binding, ATPase activity nucleus, chromosome BY4742 [00-8] B 6 YER096W SHC1 - Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication meiotic cell cycle, sporulation enzyme regulator activity membrane BY4742 [00-8] B 7 YER097W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-8] B 8 YER098W UBP9 putative ubiquitin-specific protease UBP9 Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication protein modification by small protein conjugation or removal peptidase activity, hydrolase activity cytoplasm BY4742 [00-8] B 9 YGR134W CAF130 CCR4-NOT core subunit CAF130 Subunit of the CCR4-NOT transcriptional regulatory complex; CCR4-NOT complex is evolutionarily-conserved and involved in controlling mRNA initiation, elongation, and degradation transcription from RNA polymerase II promoter, DNA-templated transcription, elongation molecular_function cytoplasm BY4742 [00-8] B 10 - cytoplasm cytoplasm cytoplasm - - - - [00-8] B 11 YGR210C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm BY4742 [00-8] B 12 YHL024W RIM4 - Putative RNA-binding protein; required for the expression of early and middle sporulation genes meiotic cell cycle, organelle fission, DNA replication, sporulation, DNA recombination RNA binding cytoplasm BY4742 [00-8] C 1 YHL025W SNF6 - Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions transcription from RNA polymerase I promoter, carbohydrate metabolic process, DNA repair, chromatin organization, rRNA processing, cellular response to DNA damage stimulus, ribosomal large subunit biogenesis, transcription from RNA polymerase II promoter, oligosaccharide metabolic process hydrolase activity, DNA binding, ATPase activity nucleus, cytoplasm BY4742 [00-8] C 2 YHR016C YSC84 LSB4 Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication cytoskeleton organization, endocytosis cytoskeletal protein binding cell cortex, cytoskeleton, cytoplasm BY4742 [00-8] C 3 YHR017W YSC83 - Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-8] C 4 YHR032W ERC1 - Member of the multi-drug and toxin extrusion (MATE) family; the MATE family is part of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine cofactor metabolic process molecular_function membrane BY4742 [00-8] C 5 YHR045W - - Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-8] C 6 YHR064C SSZ1 PDR13 Hsp70 protein that interacts with Zuo1p (a DnaJ homolog); interacts with Zuo1p to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP cytoplasmic translation, regulation of translation, rRNA processing, translational elongation unfolded protein binding cytoplasm BY4742 [00-8] C 7 YHR140W - - Putative integral membrane protein of unknown function biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-8] C 8 YHR162W MPC2 mitochondrial pyruvate carrier Highly conserved subunit of the mitochondrial pyruvate carrier (MPC); expressed during growth on fermentable carbon sources, and heterodimerizes with Mpc1p to form the fermentative isoform of MPC; MPC localizes to the mitochondrial inner membrane and mediates pyruvate uptake; MPC2 paralog, MPC3, heterodimerizes with Mpc1p to form the respiratory MPC isoform transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-8] C 9 YHR168W MTG2 putative GTPase MTG2 Putative GTPase; member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly cytoplasmic translation GTPase activity, hydrolase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-8] C 10 - membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm - - - - [00-8] C 11 - - - - - - - - [00-8] C 12 YHR181W SVP26 ERV26 Integral membrane protein of the early Golgi apparatus and ER; involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Golgi vesicle transport, protein glycosylation, cell wall organization or biogenesis protein binding, bridging cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4742 [00-8] D 1 YHR191C CTF8 - Subunit of a complex with Ctf18p; shares some subunits with Replication Factor C; required for sister chromatid cohesion mitotic cell cycle, organelle fission, chromosome segregation molecular_function chromosome BY4742 [00-8] D 2 YHR193C EGD2 - Alpha subunit of the nascent polypeptide-associated complex (NAC); involved in protein sorting and translocation; associated with cytoplasmic ribosomes protein targeting, protein folding unfolded protein binding, ion binding, lipid binding cytoplasm BY4742 [00-8] D 3 YLL030C RRT7 - Putative protein of unknown function; conserved across S. cerevisiae strains; identified in a screen for mutants with increased levels of rDNA transcription biological_process molecular_function cellular_component BY4742 [00-8] D 4 YLL044W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant biological_process molecular_function cellular_component BY4742 [00-8] D 5 YLL048C YBT1 bile acid-transporting ATPase YBT1|BAT1 Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates release of lumenal Ca2+ stores; similar to mammalian bile transporters; YBT1 has a paralog, VMR1, that arose from the whole genome duplication ion transport, lipid transport hydrolase activity, transmembrane transporter activity, ATPase activity endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-8] D 6 YLL049W LDB18 - Component of the dynactin complex; dynactin is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 mitotic cell cycle, cytoskeleton organization molecular_function cell cortex, cytoskeleton, microtubule organizing center, cytoplasm BY4742 [00-8] D 7 YLL059C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-8] D 8 YLR030W - - Putative protein of unknown function; S288C contains an in-frame stop codon between ORFs YLR030W and YLR031W biological_process molecular_function cellular_component BY4742 [00-8] D 9 YLR031W - - Putative protein of unknown function; S288C contains an in-frame stop codon between ORFs YLR030W and YLR031W; YLR031W has a paralog, YMR124W, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4742 [00-8] D 10 YLR032W RAD5 DNA helicase RAD5|SNM2|REV2 DNA helicase/Ubiquitin ligase; involved in error-free DNA damage tolerance (DDT), replication fork regression during postreplication repair by template switching, error-prone translesion synthesis; promotes synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; forms nuclear foci upon DNA replication stress; associates with native telomeres, cooperates with homologous recombination in senescent cells; human homolog HLTF can complement yeast null mutant DNA repair, cellular response to DNA damage stimulus, protein complex biogenesis, protein modification by small protein conjugation or removal hydrolase activity, DNA binding, ATPase activity, helicase activity nucleus, chromosome, cytoplasm BY4742 [00-8] D 11 - nucleus, chromosome, cytoplasm nucleus, chromosome, cytoplasm nucleus, chromosome, cytoplasm - - - - [00-8] D 12 YLR034C SMF3 putative divalent metal ion transporter SMF3 Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress cellular ion homeostasis, ion transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-8] E 1 YLR035C MLH2 mismatch repair protein MLH2 Protein involved in mismatch repair and meiotic recombination; only certain frameshift intermediates are mismatch repair substrates; forms a complex with Mlh1p meiotic cell cycle, DNA repair, organelle fission, DNA recombination, cellular response to DNA damage stimulus molecular_function nucleus BY4742 [00-8] E 2 YLR036C - - Putative protein predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein biological_process molecular_function membrane BY4742 [00-8] E 3 YLR037C PAU23 seripauperin PAU23|DAN2 Cell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth biological_process molecular_function cell wall, vacuole, cytoplasm BY4742 [00-8] E 4 YLR038C COX12 cytochrome c oxidase subunit VIb Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV mitochondrion organization, protein complex biogenesis transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-8] E 5 YLR039C RIC1 - Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes endosomal transport guanyl-nucleotide exchange factor activity nucleus, membrane, endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-8] E 6 YLR040C AFB1 - MATalpha-specific a-factor blocker; contributes to mating efficiency under certain conditions; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W response to chemical, conjugation molecular_function cell wall BY4742 [00-8] E 7 YLR041W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C biological_process molecular_function cellular_component BY4742 [00-8] E 8 YLR050C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-8] E 9 YLR052W IES3 - Subunit of the INO80 chromatin remodeling complex telomere organization, chromatin organization, DNA recombination molecular_function nucleus, chromosome BY4742 [00-8] E 10 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [00-8] E 11 YMR142C RPL13B eL13|ribosomal 60S subunit protein L13B|L13e|L13B Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-8] E 12 YMR158W MRPS8 mitochondrial 37S ribosomal protein MRPS8 Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-8] F 1 YMR171C EAR1 - Specificity factor required for Rsp5p-dependent ubiquitination; also required for sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p protein targeting, proteolysis involved in cellular protein catabolic process, endosomal transport molecular_function cytoplasmic vesicle, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-8] F 2 YMR181C - - Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4742 [00-8] F 3 YMR209C - - Putative S-adenosylmethionine-dependent methyltransferase; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; YMR209C is not an essential gene biological_process transferase activity, methyltransferase activity cellular_component BY4742 [00-8] F 4 YMR271C URA10 orotate phosphoribosyltransferase URA10 Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups, transferase activity cytoplasm BY4742 [00-8] F 5 YMR279C - - Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication transmembrane transport, ion transport transmembrane transporter activity membrane BY4742 [00-8] F 6 YMR306W FKS3 putative 1,3-beta-D-glucan synthase Protein involved in spore wall assembly; has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies meiotic cell cycle, sporulation, cell wall organization or biogenesis transferase activity, transferring glycosyl groups, transferase activity endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-8] F 7 YMR311C GLC8 - Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress carbohydrate metabolic process, generation of precursor metabolites and energy, chromosome segregation enzyme regulator activity nucleus, cytoplasm BY4742 [00-8] F 8 YMR312W ELP6 Elongator subunit ELP6|KTI4|TOT6|HAP3 Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity tRNA processing, protein modification by small protein conjugation or removal, transcription from RNA polymerase II promoter, RNA modification RNA binding, hydrolase activity, ATPase activity other BY4742 [00-8] F 9 YMR313C TGL3 bifunctional triglyceride lipase/lysophosphatidylethanolamine acyltransferase Bifunctional triacylglycerol lipase and LPE acyltransferase; major lipid particle-localized triacylglycerol (TAG) lipase; catalyzes acylation of lysophosphatidylethanolamine (LPE), a function which is essential for sporulation; protein level and stability of Tgl3p are markedly reduced in the absence of lipid droplets; required with Tgl4p for timely bud formation lipid metabolic process, cell budding transferase activity, hydrolase activity other BY4742 [00-8] F 10 - other other other - - - - [00-8] F 11 YMR315W - - Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress cofactor metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity nucleus, cytoplasm BY4742 [00-8] F 12 YMR316C-A - - Protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-8] G 1 YMR316C-B - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 5' end of ORF YMR317W biological_process molecular_function cellular_component BY4742 [00-8] G 2 YMR316W DIA1 - Protein of unknown function; involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern invasive growth in response to glucose limitation, pseudohyphal growth molecular_function cytoplasm BY4742 [00-8] G 3 YMR317W - - Putative protein of unknown function; has some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene biological_process molecular_function cellular_component BY4742 [00-8] G 4 YMR318C ADH6 NADP-dependent alcohol dehydrogenase|ADHVI NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress other oxidoreductase activity cellular_component BY4742 [00-8] G 5 YMR319C FET4 - Low-affinity Fe(II) transporter of the plasma membrane transmembrane transport, ion transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [00-8] G 6 YMR320W - - Putative protein of unknown function; conserved among S. cerevisiae strains; YMR320W is not an essential gene biological_process molecular_function cellular_component BY4742 [00-8] G 7 - cellular_component cellular_component cellular_component - - - - [00-8] G 8 YNL250W RAD50 MRX complex DNA-binding subunit Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress meiotic cell cycle, DNA repair, telomere organization, organelle fission, cellular response to DNA damage stimulus, DNA recombination, regulation of DNA metabolic process kinase activity, transferase activity, hydrolase activity, DNA binding, ATPase activity nucleus, mitochondrion, cytoplasm BY4742 [00-8] G 9 YNL252C MRPL17 mitochondrial 54S ribosomal protein YmL17/YmL30|YmL17|YmL30|MRPL30 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-8] G 10 - ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm - - - - [00-8] G 11 - - - - - - - - [00-8] G 12 YNL279W PRM1 pheromone-regulated protein PRM1 Pheromone-regulated multispanning membrane protein; involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p membrane fusion, conjugation molecular_function membrane, site of polarized growth, vacuole, cytoplasm BY4742 [00-8] H 1 - membrane, site of polarized growth, vacuole, cytoplasm membrane, site of polarized growth, vacuole, cytoplasm membrane, site of polarized growth, vacuole, cytoplasm - - - - [00-8] H 2 YNL300W TOS6 - Glycosylphosphatidylinositol-dependent cell wall protein; expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid biological_process molecular_function cell wall, cytoplasm BY4742 [00-8] H 3 - cell wall, cytoplasm cell wall, cytoplasm cell wall, cytoplasm - - - - [00-8] H 4 YNL316C PHA2 prephenate dehydratase PHA2 Prephenate dehydratase; catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway cellular amino acid metabolic process lyase activity cytoplasm BY4742 [00-8] H 5 YOL016C CMK2 calmodulin-dependent protein kinase CMK2 Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication protein phosphorylation, signaling kinase activity, transferase activity cytoplasm BY4742 [00-8] H 6 - cytoplasm cytoplasm cytoplasm - - - - [00-8] H 7 YOR096W RPS7A eS7|ribosomal 40S subunit protein S7A|S7e|rp30|S7A|RPS30 Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-8] H 8 YOR306C MCH5 - Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport ion transport other membrane, plasma membrane BY4742 [00-8] H 9 YOR317W FAA1 long-chain fatty acid-CoA ligase FAA1 Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; accounts for most acyl-CoA synthetase activity; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms ER foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4 cofactor metabolic process, ion transport, lipid transport ligase activity endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [00-8] H 10 - endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm, plasma membrane - - - - [00-8] H 11 YPL132W COX11 PSO7|LPI13 Protein required for delivery of copper to Cox1p; mitochondrial inner membrane protein; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p generation of precursor metabolites and energy, protein complex biogenesis, cellular respiration ion binding ribosome, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-8] H 12 YPL134C ODC1 mitochondrial 2-oxodicarboxylate carrier Mitochondrial inner membrane transporter; 2-oxodicarboxylate transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation; ODC1 has a paralog, ODC2, that arose from the whole genome duplication other transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-9] A 1 - membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm - - - - [00-9] A 2 - - - - - - - - [00-9] A 3 YML070W DAK1 dihydroxyacetone kinase Dihydroxyacetone kinase; required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation carbohydrate metabolic process, response to chemical kinase activity, transferase activity cytoplasm BY4742 [00-9] A 4 YML071C COG8 Golgi transport complex subunit COG8|DOR1 Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Golgi vesicle transport, protein targeting molecular_function endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-9] A 5 - endomembrane system, cytoplasm, Golgi apparatus endomembrane system, cytoplasm, Golgi apparatus endomembrane system, cytoplasm, Golgi apparatus - - - - [00-9] A 6 YML074C FPR3 peptidylprolyl isomerase FPR3|NPI46 Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; affects expression of multiple genes via its role in nucleosome assembly; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; FPR3 has a paralog, FPR4, that arose from the whole genome duplication meiotic cell cycle, organelle fission, chromatin organization, regulation of organelle organization, regulation of cell cycle isomerase activity nucleus, nucleolus BY4742 [00-9] A 7 YML090W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source biological_process molecular_function membrane BY4742 [00-9] A 8 - membrane membrane membrane - - - - [00-9] A 9 - - - - - - - - [00-9] A 10 - - - - - - - - [00-9] A 11 YML094W GIM5 PFD5 Subunit of the heterohexameric cochaperone prefoldin complex; prefoldin binds specifically to cytosolic chaperonin and transfers target proteins to it; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation transcription from RNA polymerase II promoter, protein complex biogenesis, DNA-templated transcription, elongation cytoskeletal protein binding cytoplasm BY4742 [00-9] A 12 YML095C RAD10 - Single-stranded DNA endonuclease (with Rad1p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein meiotic cell cycle, DNA repair, organelle fission, cellular response to DNA damage stimulus, DNA recombination hydrolase activity, DNA binding, nuclease activity nucleus BY4742 [00-9] B 1 YML095C-A nucleus nucleus nucleus - - - BY4742 [00-9] B 2 YML096W - putative asparagine synthase Putative protein with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 biological_process molecular_function cytoplasm BY4742 [00-9] B 3 YML097C VPS9 guanine nucleotide exchange factor VPS9|VPT9|VPL31 Guanine nucleotide exchange factor (GEF) and ubiquitin receptor; involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF MUK1; required for localization of the CORVET complex to endosomes; similar to mammalian ras inhibitors; contains a Ub-interacting CUE domain vacuole organization, protein targeting, Golgi vesicle transport, endosomal transport, organelle inheritance guanyl-nucleotide exchange factor activity, ubiquitin-like protein binding cytoplasm BY4742 [00-9] B 4 - cytoplasm cytoplasm cytoplasm - - - - [00-9] B 5 YML099C ARG81 ARGRII|ARGR2 Zinc finger transcription factor involved in arginine-responsive genes; Zn(2)-Cys(6) binuclear cluster domain type; involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p cellular amino acid metabolic process transcription factor activity, protein binding nucleus BY4742 [00-9] B 6 YML100W TSL1 trehalose 6-phosphate synthase/phosphatase complex subunit Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; contributes to survival to acute lethal heat stress; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication carbohydrate metabolic process, oligosaccharide metabolic process transferase activity, transferring glycosyl groups, phosphatase activity, transferase activity, hydrolase activity, enzyme regulator activity cytoplasm BY4742 [00-9] B 7 YML100W-A - - Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component BY4742 [00-9] B 8 YML101C CUE4 - Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE4 has a paralog, CUE1, that arose from the whole genome duplication biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-9] B 9 YML102C-A endomembrane system, endoplasmic reticulum, cytoplasm endomembrane system, endoplasmic reticulum, cytoplasm endomembrane system, endoplasmic reticulum, cytoplasm - - - BY4742 [00-9] B 10 YML102W CAC2 - Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure, deactivation of the DNA damage checkpoint after DNA repair, chromatin dynamics during transcription; and repression of divergent transcription; relocalizes to the cytosol in response to hypoxia chromatin organization histone binding nucleus, chromosome, cytoplasm BY4742 [00-9] B 11 YML103C NUP188 - Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188 response to heat, protein targeting, nucleobase-containing compound transport, nuclear transport, nucleus organization structural molecule activity nucleus, endomembrane system, membrane BY4742 [00-9] B 12 YML104C MDM1 - PtdIns-3-P binding protein that tethers the ER to vacuoles at NVJs; anchored in the ER membrane at nucleus-vacuole junctions and binds phosphatidylinositol 3-phosphate (PtdIns-3-P) in the vacuolar membrane via its Phox homology (PX) domain; expressed predominantly in late G1 to early S phase of the cell cycle; mutation affects nuclear and mitochondrial transmission to daughter buds; similar to 4 human genes, one of which (SNX14) is associated with neurological disease mitochondrion organization, organelle inheritance, nucleus organization lipid binding, ion binding vacuole, membrane, cytoplasm BY4742 [00-9] C 1 - vacuole, membrane, cytoplasm vacuole, membrane, cytoplasm vacuole, membrane, cytoplasm - - - - [00-9] C 2 YML106W URA5 orotate phosphoribosyltransferase URA5|PYR5 Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups, transferase activity nucleus, cytoplasm BY4742 [00-9] C 3 YML107C PML39 - Protein required for nuclear retention of unspliced pre-mRNAs; required along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes other molecular_function nucleus, endomembrane system, ribosome, cytoplasm BY4742 [00-9] C 4 YML108W - - Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus, cytoplasm BY4742 [00-9] C 5 YML109W ZDS2 CES4 Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintenance of Cdc55p in the cytoplasm where it promotes mitotic entry; interacts with silencing proteins at the telomere; implicated in the mitotic exit network through regulation of Cdc14p localization; ZDS2 has a paralog, ZDS1, that arose from the whole genome duplication mitotic cell cycle, chromatin organization, regulation of cell cycle molecular_function nucleus, cellular bud, site of polarized growth, cytoplasm BY4742 [00-9] C 6 YML110C COQ5 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase|DBI56 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; respiratory defect of the null mutant is partially complemented by human COQ5 cofactor metabolic process, generation of precursor metabolites and energy, cellular respiration transferase activity, methyltransferase activity mitochondrion, cytoplasm BY4742 [00-9] C 7 YML111W BUL2 ubiquitin-ubiquitin ligase BUL2 Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication protein modification by small protein conjugation or removal transferase activity cytoplasm BY4742 [00-9] C 8 YML112W CTK3 - Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress transcription from RNA polymerase I promoter, regulation of translation, protein phosphorylation, translational elongation, DNA-templated transcription, elongation molecular_function nucleus, nucleolus BY4742 [00-9] C 9 YML113W DAT1 - DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; relocalizes to the cytosol in response to hypoxia; not essential for viability transcription from RNA polymerase II promoter DNA binding nucleus, cytoplasm BY4742 [00-9] C 10 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-9] C 11 - - - - - - - - [00-9] C 12 YML116W ATR1 borate transporter|SNQ1 Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress response to chemical, transmembrane transport, ion transport transmembrane transporter activity vacuole, membrane, cytoplasm, plasma membrane BY4742 [00-9] D 1 YML117W NAB6 - Putative RNA-binding protein; associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP biological_process RNA binding, mRNA binding cytoplasm BY4742 [00-9] D 2 YML117W-A cytoplasm cytoplasm cytoplasm - - - BY4742 [00-9] D 3 YML118W NGL3 3'-5' poly(A) RNA exonuclease 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication RNA catabolic process nuclease activity, hydrolase activity cellular_component BY4742 [00-9] D 4 YML119W - - Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate biological_process molecular_function cytoplasm BY4742 [00-9] D 5 YML120C NDI1 NADH-ubiquinone reductase (H(+)-translocating) NDI1 NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in respiratory chain but does not pump protons, in contrast to higher eukaryotic multisubunit respiratory complex I; upon apoptotic stress, is activated in mitochondria by N-terminal cleavage, then translocates to cytoplasm to induce apoptosis; homolog of human AIFM2; yeast NDI1 complements several phenotypes of human cell line with mutated MT-ND4, implicated in Leber hereditary optic neuropathy cofactor metabolic process, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, cellular respiration oxidoreductase activity mitochondrion, cytoplasm BY4742 [00-9] D 6 YML121W GTR1 Rag GTPase GTR1 Subunit of a TORC1-stimulating GTPase complex; subunit of the heterodimeric Gtr1-Gtr2 GTPase complex that stimulates TORC1 in response to amino acid stimulation; tethered to the vacuolar membrane as part of the EGOC, a complex required for sorting of Gap1p and microautophagy; involved in phosphate transport and telomeric chromatin silencing; activated by the the Iml1p (GAP) subunit of the SEACIT complex; similar to human RagA and RagB transcription from RNA polymerase I promoter, chromatin organization, ion transport, transcription from RNA polymerase III promoter, signaling GTPase activity, hydrolase activity nucleus, cytoplasmic vesicle, endomembrane system, vacuole, membrane, cytoplasm BY4742 [00-9] D 7 YML122C - - Putative protein of unknown function; conserved among S. cerevisiae strains; YML122C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-9] D 8 YML123C PHO84 phosphate transporter PHO84|phoT High-affinity inorganic phosphate (Pi) transporter; also low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity transmembrane transport, ion transport transmembrane transporter activity endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [00-9] D 9 YML124C TUB3 alpha-tubulin TUB3 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication mitotic cell cycle, meiotic cell cycle, organelle fission, chromosome segregation molecular_function nucleus, cytoskeleton, microtubule organizing center, cytoplasm BY4742 [00-9] D 10 - nucleus, cytoskeleton, microtubule organizing center, cytoplasm nucleus, cytoskeleton, microtubule organizing center, cytoplasm nucleus, cytoskeleton, microtubule organizing center, cytoplasm - - - - [00-9] D 11 - - - - - - - - [00-9] D 12 - - - - - - - - [00-9] E 1 YML128C MSC1 - Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated meiotic cell cycle, organelle fission, DNA recombination molecular_function endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [00-9] E 2 YML129C COX14 - Mitochondrial cytochrome c oxidase (complex IV) assembly factor; also involved in translational regulation of Cox1p and prevention of Cox1p aggregation before assembly; associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; located in the mitochondrial membrane mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-9] E 3 - mitochondrion, membrane, mitochondrial envelope, cytoplasm mitochondrion, membrane, mitochondrial envelope, cytoplasm mitochondrion, membrane, mitochondrial envelope, cytoplasm - - - - [00-9] E 4 YML131W - - Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress biological_process molecular_function cytoplasm BY4742 [00-9] E 5 YMR004W MVP1 - Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with "foot domain" of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress protein targeting, endosomal transport lipid binding, ion binding nucleus, cytoplasmic vesicle, endomembrane system, cytoplasm BY4742 [00-9] E 6 - nucleus, cytoplasmic vesicle, endomembrane system, cytoplasm nucleus, cytoplasmic vesicle, endomembrane system, cytoplasm nucleus, cytoplasmic vesicle, endomembrane system, cytoplasm - - - - [00-9] E 7 YMR095C SNO1 putative pyridoxal 5'-phosphate synthase Protein of unconfirmed function; involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase vitamin metabolic process hydrolase activity cytoplasm BY4742 [00-9] E 8 YMR096W SNZ1 pyridoxine biosynthesis protein SNZ1 Protein involved in vitamin B6 biosynthesis; member of a stationary phase-induced gene family; coregulated with SNO1; interacts with Sno1p and with Yhr198p, perhaps as a multiprotein complex containing other Snz and Sno proteins vitamin metabolic process hydrolase activity other BY4742 [00-9] E 9 YMR097C MTG1 putative GTPase MTG1 Putative GTPase peripheral to the mitochondrial inner membrane; essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals mitochondrial translation, mitochondrion organization GTPase activity, hydrolase activity nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-9] E 10 YMR098C ATP25 LRC4 Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA; also required for the Oli1p ring formation; YMR098C is not an essential gene mitochondrion organization, protein complex biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-9] E 11 YMR099C - glucose-6-phosphate 1-epimerase Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase); likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS carbohydrate metabolic process isomerase activity nucleus, cytoplasm BY4742 [00-9] E 12 YMR100W MUB1 - MYND domain-containing protein; component of the Mub1p-Ubr2p-Rad6p ubiquitin ligase complex, required for ubiquitination and degradation of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; proposed to function as both a partner and substrate of the Ubr2p/Rad6p ubiquitin ligase; similar to the A. nidulans samB gene protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process molecular_function other BY4742 [00-9] F 1 YMR101C SRT1 ditrans,polycis-polyprenyl diphosphate synthase Forms the dehydrodolichyl diphosphate syntase (DDS) complex with NUS1; involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase protein glycosylation, lipid metabolic process transferase activity other BY4742 [00-9] F 2 YMR102C - - Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; not an essential gene; YMR102C has a paralog, DGR2, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4742 [00-9] F 3 YMR103C - - Putative protein of unknown function; conserved among S. cerevisiae strains; YMR103C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-9] F 4 - cellular_component cellular_component cellular_component - - - - [00-9] F 5 YMR105C PGM2 phosphoglucomutase PGM2|GAL5 Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress; PGM2 has a paralog, PGM1, that arose from the whole genome duplication carbohydrate metabolic process, cellular ion homeostasis, generation of precursor metabolites and energy, oligosaccharide metabolic process isomerase activity cytoplasm BY4742 [00-9] F 6 YMR106C YKU80 ATP-dependent DNA helicase YKU80|HDF2 Subunit of telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein via interaction with TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair; colocalizes with quiescent cell telomere hyperclusters DNA repair, telomere organization, chromatin organization, cellular response to DNA damage stimulus, DNA recombination RNA binding, DNA binding nucleus, chromosome BY4742 [00-9] F 7 YMR107W SPG4 - Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources biological_process molecular_function cellular_component BY4742 [00-9] F 8 - cellular_component cellular_component cellular_component - - - - [00-9] F 9 YMR109W MYO5 myosin 5 One of two type I myosin motors; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO5 has a paralog, MYO3, that arose from the whole genome duplication mitotic cell cycle, cell budding, cytokinesis, exocytosis, cell wall organization or biogenesis, response to osmotic stress, endocytosis hydrolase activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [00-9] F 10 YMR110C HFD1 hexadecenal dehydrogenase Dehydrogenase involved in ubiquinone and sphingolipid metabolism; oxidizes 4-hydroxybenzaldehyde into 4-hydroxybenzoic acid in ubiquinone biosynthesis; converts hexadecenal to hexadecenoic acid in sphingosine 1-phosphate breakdown pathway; located in the mitochondrial outer membrane and also in lipid particles; human homolog ALDH3A2, a fatty aldehyde dehydrogenase (FALDH) mutated in neurocutaneous disorder Sjogren-Larsson syndrome, can complement yeast hfd1 mutant cofactor metabolic process, cellular amino acid metabolic process, lipid metabolic process oxidoreductase activity cytoplasmic vesicle, endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm BY4742 [00-9] F 11 YMR111C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress biological_process molecular_function nucleus, cytoplasm BY4742 [00-9] F 12 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-9] G 1 - - - - - - - - [00-9] G 2 YMR114C - putative peptide hydrolase Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene biological_process molecular_function nucleus, ribosome, cytoplasm BY4742 [00-9] G 3 YMR115W MGR3 FMP24 Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA proteolysis involved in cellular protein catabolic process other membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-9] G 4 YMR116C ASC1 RACK1|guanine nucleotide-binding protein subunit beta|ASU9|NAD1|CPC2 G-protein beta subunit and guanine dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; required to prevent frameshifting at ribosomes stalled at repeated CGA codons; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation response to chemical, regulation of translation, invasive growth in response to glucose limitation, translational elongation, signaling enzyme regulator activity, signal transducer activity ribosome, cytoplasm BY4742 [00-9] G 5 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [00-9] G 6 - - - - - - - - [00-9] G 7 - - - - - - - - [00-9] G 8 YMR119W-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of ORF ASI1/YMR119W biological_process molecular_function cellular_component BY4742 [00-9] G 9 YMR120C ADE17 bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase ADE17 Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine nucleobase-containing small molecule metabolic process transferase activity, hydrolase activity membrane, plasma membrane, cytoplasm BY4742 [00-9] G 10 YMR121C RPL15B eL15|ribosomal 60S subunit protein L15B|L15e|rp15R|YL10|L15B|L13B Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome nucleus, nucleolus, ribosome, cytoplasm BY4742 [00-9] G 11 YMR122C - - Putative protein of unknown function; conserved among S. cerevisiae strains; YMR122C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-9] G 12 YMR123W PKR1 - V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress protein complex biogenesis molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-9] H 1 YMR124W EPO1 - Protein involved in septin-ER tethering; interacts with ER membrane protein, Scs2p, and Shs1p, a septin ring component, at bud neck to create ER diffusion barrier; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W has a paralog, YLR031W, that arose from the whole genome duplication other molecular_function cellular bud, site of polarized growth, cytoplasm BY4742 [00-9] H 2 YMR125W STO1 SUT1|CBP80|GCR3|CBC1 Large subunit of the nuclear mRNA cap-binding protein complex; interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 RNA catabolic process, RNA splicing, mRNA processing, response to osmotic stress RNA binding, mRNA binding nucleus BY4742 [00-9] H 3 YMR126C DLT1 - Protein of unknown function; mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) biological_process molecular_function cellular_component BY4742 [00-9] H 4 YMR127C SAS2 KAT8 Histone acetyltransferase (HAT) catalytic subunit of the SAS complex; acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family; other members are Sas4p and Sas5p peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity nucleus, chromosome BY4742 [00-9] H 5 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [00-9] H 6 YMR129W POM152 - Glycoprotein subunit of transmembrane ring of nuclear pore complex; contributes to nucleocytoplasmic transport, nuclear pore complex (NPC) biogenesis and spindle pole body duplication; homologous to human NUP210 protein targeting, cytoskeleton organization, nuclear transport, nucleus organization structural molecule activity nucleus, endomembrane system, mitochondrion, membrane, cytoplasm BY4742 [00-9] H 7 YMR130W - - Putative protein of unknown function; YMR130W is not an essential gene biological_process molecular_function cellular_component BY4742 [00-9] H 8 - cellular_component cellular_component cellular_component - - - - [00-9] H 9 YMR132C JLP2 - Protein of unknown function; contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) biological_process molecular_function cytoplasm BY4742 [00-9] H 10 YMR133W REC114 - Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division meiotic cell cycle, organelle fission, DNA recombination molecular_function nucleus, chromosome BY4742 [00-9] H 11 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [00-9] H 12 YMR135C GID8 glucose-induced degradation complex subunit GID8|DCR1 Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START mitotic cell cycle, carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process, regulation of cell cycle molecular_function nucleus, cytoplasm BY4742 [00-10] A 1 YEL012W UBC8 E2 ubiquitin-conjugating protein UBC8|GID3 Ubiquitin-conjugating enzyme that regulates gluconeogenesis; negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro carbohydrate metabolic process, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process transferase activity cytoplasm BY4742 [00-10] A 2 - cytoplasm cytoplasm cytoplasm - - - - [00-10] A 3 - - - - - - - - [00-10] A 4 - - - - - - - - [00-10] A 5 - - - - - - - - [00-10] A 6 - - - - - - - - [00-10] A 7 YER031C YPT31 Rab family GTPase YPT31|YPT8 Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication; localizes to the transitional and late Golgi Golgi vesicle transport, exocytosis, endosomal transport GTPase activity, hydrolase activity cytoplasmic vesicle, endomembrane system, mitochondrion, membrane, mitochondrial envelope, Golgi apparatus, cytoplasm BY4742 [00-10] A 8 YER046W SPO73 - Meiosis-specific protein required for prospore membrane morphogenesis; required for the proper shape of the prospore membrane (PSM) and for spore wall formation; functions cooperatively with SPO71 in PSM elongation; physically interacts with Spo71p; genetically antagonistic to SPO1, similar to SPO71; localizes to the PSM; required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis; dysferlin domain-only protein meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function membrane, cytoplasm BY4742 [00-10] A 9 YER063W THO1 - Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation nucleobase-containing compound transport, nuclear transport chromatin binding, RNA binding, DNA binding nucleus BY4742 [00-10] A 10 YER066W RRT13 - Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription biological_process molecular_function cellular_component BY4742 [00-10] A 11 - cellular_component cellular_component cellular_component - - - - [00-10] A 12 - - - - - - - - [00-10] B 1 YGR188C BUB1 protein kinase BUB1 Protein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication mitotic cell cycle, protein phosphorylation, organelle fission, regulation of organelle organization, chromosome segregation, regulation of cell cycle kinase activity, transferase activity nucleus, chromosome BY4742 [00-10] B 2 YGR201C - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-10] B 3 YGR204W ADE3 trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase ADE3 Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase; involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine cofactor metabolic process, nucleobase-containing small molecule metabolic process ligase activity, hydrolase activity, oxidoreductase activity nucleus, cytoplasm BY4742 [00-10] B 4 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-10] B 5 YHL002W HSE1 ESCRT-0 subunit protein HSE1 Subunit of the endosomal Vps27p-Hse1p complex; complex is required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes protein targeting, endosomal transport, ion transport, nucleobase-containing compound transport ubiquitin-like protein binding cytoplasmic vesicle, endomembrane system, cytoplasm BY4742 [00-10] B 6 - cytoplasmic vesicle, endomembrane system, cytoplasm cytoplasmic vesicle, endomembrane system, cytoplasm cytoplasmic vesicle, endomembrane system, cytoplasm - - - - [00-10] B 7 YHL011C PRS3 ribose phosphate diphosphokinase subunit PRS3 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes cell wall organization or biogenesis transferase activity cytoplasm BY4742 [00-10] B 8 - cytoplasm cytoplasm cytoplasm - - - - [00-10] B 9 YHL039W EFM1 protein-lysine N-methyltransferase Lysine methyltransferase; involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm peptidyl-amino acid modification, protein alkylation transferase activity, methyltransferase activity cytoplasm BY4742 [00-10] B 10 - cytoplasm cytoplasm cytoplasm - - - - [00-10] B 11 - - - - - - - - [00-10] B 12 YHR003C TCD1 tRNA threonylcarbamoyladenosine dehydratase tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD1 has a paralog, TCD2, that arose from the whole genome duplication tRNA processing, RNA modification lyase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-10] C 1 YHR004C NEM1 Nem1-Spo7 phosphatase catalytic subunit NEM1 Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard protein dephosphorylation, lipid metabolic process, nucleus organization phosphatase activity, hydrolase activity endomembrane system, mitochondrion, membrane, cytoplasm BY4742 [00-10] C 2 YHR005C GPA1 guanine nucleotide-binding protein subunit alpha|SCG1|DAC1|CDC70 Subunit of the G protein involved in pheromone response; GTP-binding alpha subunit of the heterotrimeric G protein; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates Vps34p at the endosome; protein abundance increases in response to DNA replication stress response to chemical, regulation of transport, organelle fusion, conjugation, nuclear transport, signaling, nucleus organization GTPase activity, hydrolase activity cytoplasmic vesicle, endomembrane system, membrane, cytoplasm, plasma membrane BY4742 [00-10] C 3 YHR006W STP2 - Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [00-10] C 4 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-10] C 5 YHR008C SOD2 superoxide dismutase SOD2 Mitochondrial manganese superoxide dismutase; protects cells against oxygen toxicity and oxidative stress; human mitochondrial SOD2 can complement a yeast null mutant and human cytoplasmic SOD1 can also complement when targeted to the mitochondrial matrix response to chemical, response to oxidative stress oxidoreductase activity mitochondrion, cytoplasm BY4742 [00-10] C 6 YHR009C TDA3 BTN3 Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 endosomal transport molecular_function cytoplasmic vesicle, endomembrane system, cytoplasm BY4742 [00-10] C 7 - cytoplasmic vesicle, endomembrane system, cytoplasm cytoplasmic vesicle, endomembrane system, cytoplasm cytoplasmic vesicle, endomembrane system, cytoplasm - - - - [00-10] C 8 YHR025W THR1 homoserine kinase Homoserine kinase; conserved protein required for threonine biosynthesis; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic filaments; expression is regulated by the GCN4-mediated general amino acid control pathway cellular amino acid metabolic process kinase activity, transferase activity cellular_component BY4742 [00-10] C 9 YHR026W VMA16 H(+)-transporting V0 sector ATPase subunit c''|PPA1 Subunit c'' of the vacuolar ATPase; v-ATPase functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain cellular ion homeostasis ATPase activity, hydrolase activity, transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-10] C 10 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [00-10] C 11 YHR041C SRB2 MED20|HRS2 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance response to chemical, DNA-templated transcription, initiation, transcription from RNA polymerase II promoter transcription factor activity, protein binding nucleus BY4742 [00-10] C 12 - nucleus nucleus nucleus - - - - [00-10] D 1 YHR059W FYV4 - Protein of unknown function; required for survival upon exposure to K1 killer toxin biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-10] D 2 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-10] D 3 YHR067W HTD2 hydroxyacyl-thioester dehydratase HTD2|RMD12 Mitochondrial 3-hydroxyacyl-thioester dehydratase; involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology lipid metabolic process, monocarboxylic acid metabolic process lyase activity mitochondrion, cytoplasm BY4742 [00-10] D 4 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-10] D 5 - - - - - - - - [00-10] D 6 - - - - - - - - [00-10] D 7 - - - - - - - - [00-10] D 8 - - - - - - - - [00-10] D 9 - - - - - - - - [00-10] D 10 - - - - - - - - [00-10] D 11 YHR127W - HSN1 Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis mitotic cell cycle, organelle fission, cytoskeleton organization, chromosome segregation molecular_function nucleus BY4742 [00-10] D 12 - nucleus nucleus nucleus - - - - [00-10] E 1 YHR131C - - Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study; YHR131C has a paralog, YNL144C, that arose from the whole genome duplication biological_process molecular_function cytoplasm BY4742 [00-10] E 2 - cytoplasm cytoplasm cytoplasm - - - - [00-10] E 3 - - - - - - - - [00-10] E 4 - - - - - - - - [00-10] E 5 - - - - - - - - [00-10] E 6 - - - - - - - - [00-10] E 7 - - - - - - - - [00-10] E 8 - - - - - - - - [00-10] E 9 YHR180W - - Putative protein of unknown function; conserved among S. cerevisiae strains biological_process molecular_function cellular_component BY4742 [00-10] E 10 YHR185C PFS1 ADY1 Sporulation protein required for prospore membrane formation; required for prospore membrane formation at selected spindle poles; ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function cellular_component BY4742 [00-10] E 11 - cellular_component cellular_component cellular_component - - - - [00-10] E 12 - - - - - - - - [00-10] F 1 YHR194W MDM31 - Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 mitochondrion organization, cellular ion homeostasis, lipid metabolic process, organelle inheritance molecular_function nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-10] F 2 - nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm - - - - [00-10] F 3 - - - - - - - - [00-10] F 4 - - - - - - - - [00-10] F 5 YLL007C LMO1 - Homolog of mammalian ELMO (Engulfment and celL MOtility); upstream component for regulation through the small GTPase Rho5p; may form a complex with Dck1p that acts as a GEF for Rho5p; cytoplasmic protein that relocates to mitochondria under oxidative stress; implicated in mitophagy; not an essential protein mitochondrion organization, signaling molecular_function membrane, mitochondrion, plasma membrane, cytoplasm BY4742 [00-10] F 6 - membrane, mitochondrion, plasma membrane, cytoplasm membrane, mitochondrion, plasma membrane, cytoplasm membrane, mitochondrion, plasma membrane, cytoplasm - - - - [00-10] F 7 YMR154C RIM13 CPL1 Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB protein maturation peptidase activity, hydrolase activity cellular_component BY4742 [00-10] F 8 - cellular_component cellular_component cellular_component - - - - [00-10] F 9 YNL274C GOR1 glyoxylate reductase Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress monocarboxylic acid metabolic process oxidoreductase activity nucleus, mitochondrion, cytoplasm BY4742 [00-10] F 10 - nucleus, mitochondrion, cytoplasm nucleus, mitochondrion, cytoplasm nucleus, mitochondrion, cytoplasm - - - - [00-10] F 11 - - - - - - - - [00-10] F 12 - - - - - - - - [00-10] G 1 - - - - - - - - [00-10] G 2 YOL141W PPM2 tRNA methyltransferase PPM2|TYW4 AdoMet-dependent tRNA methyltransferase; also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p tRNA processing, RNA modification transferase activity, methyltransferase activity mitochondrion, cytoplasm BY4742 [00-10] G 3 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-10] G 4 YOL143C RIB4 lumazine synthase RIB4 Lumazine synthase (DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin; DMRL synthase stands for 6,7-dimethyl-8-ribityllumazine synthase vitamin metabolic process isomerase activity nucleus, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-10] G 5 - nucleus, mitochondrial envelope, mitochondrion, cytoplasm nucleus, mitochondrial envelope, mitochondrion, cytoplasm nucleus, mitochondrial envelope, mitochondrion, cytoplasm - - - - [00-10] G 6 - - - - - - - - [00-10] G 7 - - - - - - - - [00-10] G 8 - - - - - - - - [00-10] G 9 - - - - - - - - [00-10] G 10 - - - - - - - - [00-10] G 11 YOL150C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-10] G 12 - cellular_component cellular_component cellular_component - - - - [00-10] H 1 - - - - - - - - [00-10] H 2 - - - - - - - - [00-10] H 3 - - - - - - - - [00-10] H 4 YOL158C ENB1 ARN4 Endosomal ferric enterobactin transporter; expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment ion transport transmembrane transporter activity cellular bud, membrane, vacuole, plasma membrane, cytoplasm BY4742 [00-10] H 5 YOL159C CSS3 - Protein of unknown function, secreted when constitutively expressed; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the cell periphery, SWAT-GFP fusion also localizes to the extracellular region, and mCherry fusion also localizes to the vacuole; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA biological_process molecular_function extracellular region BY4742 [00-10] H 6 YOL160W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-10] H 7 YOL162W - - Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family transmembrane transport transmembrane transporter activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-10] H 8 YOL163W - - Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family transmembrane transport transmembrane transporter activity membrane BY4742 [00-10] H 9 - membrane membrane membrane - - - - [00-10] H 10 - - - - - - - - [00-10] H 11 - - - - - - - - [00-10] H 12 YOR008C-A - - Putative protein of unknown function; includes a potential transmembrane domain; deletion results in slightly lengthened telomeres; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively biological_process molecular_function cellular_component BY4742 [00-11] A 1 - cellular_component cellular_component cellular_component - - - - [00-11] A 2 - - - - - - - - [00-11] A 3 - - - - - - - - [00-11] A 4 - - - - - - - - [00-11] A 5 - - - - - - - - [00-11] A 6 - - - - - - - - [00-11] A 7 YBR191W RPL21A eL21|ribosomal 60S subunit protein L21A|L21e|L21A|URP1 Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-11] A 8 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [00-11] A 9 YCL035C GRX1 dithiol glutaredoxin GRX1 Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress response to chemical, response to oxidative stress transferase activity, oxidoreductase activity nucleus, cytoplasm BY4742 [00-11] A 10 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-11] A 11 - - - - - - - - [00-11] A 12 - - - - - - - - [00-11] B 1 - - - - - - - - [00-11] B 2 - - - - - - - - [00-11] B 3 - - - - - - - - [00-11] B 4 - - - - - - - - [00-11] B 5 - - - - - - - - [00-11] B 6 - - - - - - - - [00-11] B 7 YDR071C PAA1 polyamine acetyltransferase Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication chromatin organization transferase activity cytoplasm BY4742 [00-11] B 8 YDR074W TPS2 trehalose-phosphatase TPS2|PFK3|HOG2 Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress response to heat, carbohydrate metabolic process, oligosaccharide metabolic process phosphatase activity, hydrolase activity mitochondrion, cytoplasm BY4742 [00-11] B 9 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-11] B 10 YER027C GAL83 SPM1 One of three possible beta-subunits of the Snf1 kinase complex; allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; necessary and sufficient for phosphorylation of the Mig2p transcription factor in response to alkaline stress; functionally redundant with SIP1 and SIP2 for the phosphorylation of Mig1p in response to glucose deprivation; contains a glycogen-binding domain protein phosphorylation, invasive growth in response to glucose limitation, protein complex biogenesis, signaling kinase activity, transferase activity nucleus, endomembrane system, cytoplasm BY4742 [00-11] B 11 YER037W PHM8 bifunctional nucleotidase/lysophosphatidic acid phosphatase Lysophosphatidic acid (LPA) phosphatase, nucleotidase; principle and physiological nucleotidase working on GMP, UMP and CMP; involved in LPA hydrolysis in response to phosphate starvation and ribose salvage pathway; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p; repressed by Gcn4p under normal conditions; PHM8 has a paralog, SDT1, that arose from the whole genome duplication response to starvation, nucleobase-containing small molecule metabolic process phosphatase activity, hydrolase activity nucleus, cytoplasm BY4742 [00-11] B 12 YGR155W CYS4 cystathionine beta-synthase CYS4|VMA41|STR4|NHS5 Cystathionine beta-synthase; catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog CBS cause homocystinuria; human CBS can complement yeast null mutant mitotic cell cycle, cellular amino acid metabolic process, regulation of cell cycle RNA binding, lyase activity, mRNA binding mitochondrion, cytoplasm BY4742 [00-11] C 1 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-11] C 2 - - - - - - - - [00-11] C 3 - - - - - - - - [00-11] C 4 - - - - - - - - [00-11] C 5 - - - - - - - - [00-11] C 6 - - - - - - - - [00-11] C 7 - - - - - - - - [00-11] C 8 YLR192C HCR1 translation initiation factor eIF3 core subunit j eIF3j component of translation initiation factor 3 (eIF3); dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of eIF3; required for 20S pre-rRNA processing; required at post-transcriptional step for efficient retrotransposition; absence decreases Ty1 Gag:GFP protein levels; binds eIF3 subunits Rpg1p, Prt1p and 18S rRNA; eIF3 also involved in programmed stop codon read through; human homolog EIF3J can complement yeast hcr1 mutant cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis RNA binding, translation factor activity, RNA binding cytoplasm BY4742 [00-11] C 9 - cytoplasm cytoplasm cytoplasm - - - - [00-11] C 10 YLR237W THI7 thiamine transporter THI7|THI10 Plasma membrane transporter responsible for the uptake of thiamine; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia transmembrane transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-11] C 11 YLR246W ERF2 palmitoyltransferase ERF2 Subunit of a palmitoyltransferase; this complex adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole; palmitoyltransferase is composed of Erf2p and Shr5p protein targeting, protein acylation, protein lipidation transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-11] C 12 - endomembrane system, membrane, endoplasmic reticulum, cytoplasm endomembrane system, membrane, endoplasmic reticulum, cytoplasm endomembrane system, membrane, endoplasmic reticulum, cytoplasm - - - - [00-11] D 1 YLR334C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here biological_process molecular_function membrane BY4742 [00-11] D 2 - membrane membrane membrane - - - - [00-11] D 3 YLR346C CIS1 - Protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; not an essential gene; YLR346C has a paralog, YGR035C, that arose from the whole genome duplication biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-11] D 4 YLR358C - - Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W biological_process molecular_function cellular_component BY4742 [00-11] D 5 YLR361C DCR2 phosphoprotein phosphatase Protein phosphatase; involved in downregulation of the unfolded protein response (UPR), at least in part through dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START; physically interacts with, dephosphorylates and destabilizes Sic1p; SWAT-GFP and mCherry fusion proteins localize to the vacuole mitotic cell cycle, response to chemical, protein dephosphorylation, signaling, regulation of cell cycle phosphatase activity, hydrolase activity vacuole, cytoplasm BY4742 [00-11] D 6 YLR370C ARC18 - Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches organelle inheritance, mitochondrion organization, cytoskeleton organization molecular_function cytoskeleton, mitochondrion, cytoplasm BY4742 [00-11] D 7 YLR382C NAM2 leucine--tRNA ligase NAM2|LeuRS|MSL1 Mitochondrial leucyl-tRNA synthetase; also has direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance; human homolog LARS2 can complement yeast null mutant, and is implicated in Perrault syndrome mitochondrial translation, RNA splicing, mitochondrion organization, cellular amino acid metabolic process, tRNA aminoacylation for protein translation RNA binding, ligase activity, mRNA binding mitochondrion, cytoplasm BY4742 [00-11] D 8 YLR394W CST9 SUMO ligase CST9|ZIP3 SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; associates with centromeres early in meiosis, then with chromosome axes and finally with double-strand break sites that are engaged in repair by crossovers; potential Cdc28p substrate peptidyl-amino acid modification, meiotic cell cycle, organelle fission, chromosome segregation, protein modification by small protein conjugation or removal, DNA recombination chromatin binding, transferase activity nucleus, chromosome BY4742 [00-11] D 9 YLR406C RPL31B eL31|ribosomal 60S subunit protein L31B|L31e|YL28|L34B|L31B Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication cytoplasmic translation, regulation of translation, translational elongation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-11] D 10 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [00-11] D 11 YML022W APT1 adenine phosphoribosyltransferase APT1 Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups, transferase activity nucleus, cytoplasm BY4742 [00-11] D 12 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-11] E 1 YML027W YOX1 - Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; phosphorylated by Cdc28p; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication mitotic cell cycle DNA binding, transcription factor activity, protein binding nucleus, chromosome, cytoplasm BY4742 [00-11] E 2 - nucleus, chromosome, cytoplasm nucleus, chromosome, cytoplasm nucleus, chromosome, cytoplasm - - - - [00-11] E 3 - - - - - - - - [00-11] E 4 - - - - - - - - [00-11] E 5 YML036W CGI121 - Component of the EKC/KEOPS complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; Cgi121p is dispensable for tRNA modification; other complex members are Bud32p, Kae1p, Pcc1p, and Gon7p telomere organization, DNA recombination, transcription from RNA polymerase II promoter molecular_function other BY4742 [00-11] E 6 YML038C YMD8 - Putative nucleotide sugar transporter; has similarity to Vrg4p ion transport, nucleobase-containing compound transport transmembrane transporter activity membrane, cytoplasmic vesicle, endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-11] E 7 YML041C VPS71 SWC6 Nucleosome-binding component of the SWR1 complex; SWR1 exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting protein targeting, chromatin organization chromatin binding nucleus, chromosome BY4742 [00-11] E 8 YML042W CAT2 carnitine O-acetyltransferase CAT2|YCAT Carnitine acetyl-CoA transferase; present in both mitochondria and peroxisomes; transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes other transferase activity peroxisome, mitochondrion, cytoplasm BY4742 [00-11] E 9 YML047C PRM6 pheromone-regulated K(+) transporter PRM6|KCH2 Potassium transporter that mediates K+ influx; activates high-affinity Ca2+ influx system (HACS) during mating pheromone response; expression up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; member of a fungal-specific gene family; PRM6 has a paralog, KCH1, that arose from the whole genome duplication transmembrane transport, ion transport transmembrane transporter activity vacuole, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane BY4742 [00-11] E 10 - vacuole, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane vacuole, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane vacuole, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane - - - - [00-11] E 11 - - - - - - - - [00-11] E 12 - - - - - - - - [00-11] F 1 YML075C HMG1 hydroxymethylglutaryl-CoA reductase (NADPH) HMG1 HMG-CoA reductase; catalyzes conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to nuclear envelope; overproduction induces formation of karmellae; forms foci at nuclear periphery upon DNA replication stress; HMG1 has a paralog, HMG2, that arose from the whole genome duplication; human homolog HMGCR can complement yeast hmg1 mutant cofactor metabolic process, lipid metabolic process oxidoreductase activity nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-11] F 2 YML076C WAR1 - Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, mitochondrion, cytoplasm BY4742 [00-11] F 3 - nucleus, mitochondrion, cytoplasm nucleus, mitochondrion, cytoplasm nucleus, mitochondrion, cytoplasm - - - - [00-11] F 4 YML086C ALO1 D-arabinono-1,4-lactone oxidase D-Arabinono-1,4-lactone oxidase; catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress response to chemical, response to oxidative stress oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-11] F 5 - membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm - - - - [00-11] F 6 - - - - - - - - [00-11] F 7 - - - - - - - - [00-11] F 8 YMR048W CSM3 - Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress mitotic cell cycle, meiotic cell cycle, DNA repair, organelle fission, DNA replication, cellular response to DNA damage stimulus, chromosome segregation, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, chromosome, cytoplasm BY4742 [00-11] F 9 YMR135W-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-11] F 10 - cellular_component cellular_component cellular_component - - - - [00-11] F 11 YMR137C PSO2 SNM1 Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress DNA repair, cellular response to DNA damage stimulus DNA binding, nuclease activity, hydrolase activity nucleus BY4742 [00-11] F 12 YMR138W CIN4 Arf family GTPase CIN4|UGX1|GTP1 GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog protein folding, protein complex biogenesis molecular_function cytoplasm BY4742 [00-11] G 1 YMR139W RIM11 serine/threonine protein kinase RIM11|MDS1|GSK3 Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress; RIM11 has a paralog, MRK1, that arose from the whole genome duplication response to heat, meiotic cell cycle, protein phosphorylation, sporulation, response to osmotic stress kinase activity, transferase activity cytoplasm BY4742 [00-11] G 2 YMR160W - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-11] G 3 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [00-11] G 4 YMR173W DDR48 DNA damage-responsive protein 48|FSP DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress DNA repair, cellular response to DNA damage stimulus GTPase activity, ATPase activity, hydrolase activity cytoplasm BY4742 [00-11] G 5 - cytoplasm cytoplasm cytoplasm - - - - [00-11] G 6 - - - - - - - - [00-11] G 7 YMR198W CIK1 - Kinesin-associated protein; required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; locus encodes a long and short transcript with differing functions; CIK1 has a paralog, VIK1, that arose from the whole genome duplication mitotic cell cycle, meiotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, organelle fusion, chromosome segregation, conjugation, regulation of cell cycle, nucleus organization hydrolase activity, ATPase activity nucleus, cytoskeleton, microtubule organizing center, cytoplasm BY4742 [00-11] G 8 - nucleus, cytoskeleton, microtubule organizing center, cytoplasm nucleus, cytoskeleton, microtubule organizing center, cytoplasm nucleus, cytoskeleton, microtubule organizing center, cytoplasm - - - - [00-11] G 9 YOR298C-A MBF1 SUF13 Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress transcription from RNA polymerase II promoter transcription factor activity, protein binding nucleus, mitochondrion, cytoplasm BY4742 [00-11] G 10 - nucleus, mitochondrion, cytoplasm nucleus, mitochondrion, cytoplasm nucleus, mitochondrion, cytoplasm - - - - [00-11] G 11 - - - - - - - - [00-11] G 12 - - - - - - - - [00-11] H 1 YOR364W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C biological_process molecular_function cellular_component BY4742 [00-11] H 2 - cellular_component cellular_component cellular_component - - - - [00-11] H 3 - - - - - - - - [00-11] H 4 YPL148C PPT2 holo-[acyl-carrier-protein] synthase Phosphopantetheine:protein transferase (PPTase); activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation other transferase activity mitochondrion, cytoplasm BY4742 [00-11] H 5 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-11] H 6 YPL183C RTT10 TRM734|ERE2 WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 endosomal transport molecular_function cytoplasmic vesicle, endomembrane system, cytoplasm BY4742 [00-11] H 7 YPL183W-A RTC6 putative mitochondrial 54S ribosomal protein RTC6|TAE4|GON5 Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-11] H 8 YPL189W GUP2 putative O-acyltransferase Probable membrane protein; possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; homolog of the mammalian Hedgehog pathway modulator HHAT; GUP2 has a paralog, GUP1, that arose from the whole genome duplication carbohydrate transport transferase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-11] H 9 - membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm - - - - [00-11] H 10 - - - - - - - - [00-11] H 11 YPL224C MMT2 MFT2 Putative metal transporter involved in mitochondrial iron accumulation; MMT2 has a paralog, MMT1, that arose from the whole genome duplication cellular ion homeostasis molecular_function membrane, mitochondrion, cytoplasm BY4742 [00-11] H 12 - membrane, mitochondrion, cytoplasm membrane, mitochondrion, cytoplasm membrane, mitochondrion, cytoplasm - - - - [00-12] A 1 YAL012W CYS3 cystathionine gamma-lyase CYS3|STR1|FUN35|CYI1 Cystathionine gamma-lyase; catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress cellular amino acid metabolic process lyase activity nucleus, cytoplasm BY4742 [00-12] A 2 YAL016W TPD3 protein phosphatase 2A structural subunit TPD3|FUN32 Regulatory subunit A of the heterotrimeric PP2A complex; the heterotrimeric protein phosphatase 2A (PP2A) complex also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and transcription by RNA polymerase III mitotic cell cycle, regulation of translation, organelle fission, regulation of organelle organization, chromosome segregation, regulation of cell cycle phosphatase activity, hydrolase activity nucleus, cytoskeleton, microtubule organizing center, cellular bud, site of polarized growth, cytoplasm BY4742 [00-12] A 3 YAL047C SPC72 gamma-tubulin complex subunit SPC72|LDB4 Gamma-tubulin small complex (gamma-TuSC) receptor; recruits the gamma-TuSC complex to the cytoplasmic side of the SPB, connecting nuclear microtubules to the SPB; involved in astral microtubule formation, stabilization, and with Stu2p, anchoring astral MTs at the cytoplasmic face of the SPB, and regulating plus-end MT dynamics; regulated by Cdc5 kinase mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, protein complex biogenesis, chromosome segregation, regulation of cell cycle structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm BY4742 [00-12] A 4 YAL054C ACS1 acetate--CoA ligase 1|FUN44 Acetyl-coA synthetase isoform; along with Acs2p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions peptidyl-amino acid modification, protein acylation, cofactor metabolic process, chromatin organization, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, histone modification ligase activity mitochondrion, membrane, cytoplasm BY4742 [00-12] A 5 YAL056C-A - YAL058C-A Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-12] A 6 YAR050W FLO1 flocculin FLO1|FLO4|FLO2 Lectin-like protein involved in flocculation; cell wall protein that binds mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; important for co-flocculation with other yeasts, mediating interaction with specific species; FLO1 has a paralog, FLO5, that arose from a segmental duplication response to heat, response to chemical, response to oxidative stress other cell wall BY4742 [00-12] A 7 YCL006C cell wall cell wall cell wall - - - BY4742 [00-12] A 8 - - - - - - - - [00-12] A 9 - - - - - - - - [00-12] A 10 YCL022C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W biological_process molecular_function cellular_component BY4742 [00-12] A 11 YCL023C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 biological_process molecular_function cellular_component BY4742 [00-12] A 12 YCL038C ATG22 AUT4 Vacuolar integral membrane protein required for efflux of amino acids; required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation transmembrane transport, ion transport, amino acid transport molecular_function membrane, vacuole, cytoplasm BY4742 [00-12] B 1 YCL058C FYV5 MDF1 Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis response to chemical, cellular ion homeostasis, conjugation, signaling molecular_function membrane, cytoplasm BY4742 [00-12] B 2 YCL074W membrane, cytoplasm membrane, cytoplasm membrane, cytoplasm - - - BY4742 [00-12] B 3 YCL075W - - - - - - BY4742 [00-12] B 4 YCL076W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-12] B 5 YGL199C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W biological_process molecular_function cellular_component BY4742 [00-12] B 6 YGL214W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C biological_process molecular_function cellular_component BY4742 [00-12] B 7 YGL217C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W biological_process molecular_function cellular_component BY4742 [00-12] B 8 YGL218W - SRF1 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance biological_process molecular_function cellular_component BY4742 [00-12] B 9 YGL235W - - Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 biological_process molecular_function cellular_component BY4742 [00-12] B 10 - cellular_component cellular_component cellular_component - - - - [00-12] B 11 YGR011W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-12] B 12 YGR018C - - Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W biological_process molecular_function cellular_component BY4742 [00-12] C 1 YGR022C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W biological_process molecular_function cellular_component BY4742 [00-12] C 2 YGR025W - - Putative protein of unknown function; conserved across S. cerevisiae strains biological_process molecular_function cellular_component BY4742 [00-12] C 3 - cellular_component cellular_component cellular_component - - - - [00-12] C 4 - - - - - - - - [00-12] C 5 YJR069C HAM1 nucleoside triphosphate pyrophosphohydrolase HAM1 Nucleoside triphosphate pyrophosphohydrolase; active against various substrates including ITP, dITP and XTP; mediates exclusion of non canonical purines, pyrimidines from dNTP pools; functions with YJL055W to mediate resistance to 5-FU; specifically reduces the incorporation of 5-FU into RNA without affecting uptake or incorporation of uracil into RNA; protein abundance increases in response to DNA replication stress; yeast HAM1 can complement knockdown of human homolog ITPA nucleobase-containing small molecule metabolic process hydrolase activity nucleus, cytoplasm BY4742 [00-12] C 6 YJR070C LIA1 MMD1 Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress peptidyl-amino acid modification, cytoskeleton organization oxidoreductase activity nucleus, cytoplasm BY4742 [00-12] C 7 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-12] C 8 YJR074W MOG1 Ran GTPase-binding protein MOG1 Conserved nuclear protein that interacts with GTP-Gsp1p; stimulates nucleotide release from Gsp1p; involved in nuclear protein import; nucleotide release is inhibited by Yrb1p protein targeting, nuclear transport enzyme binding nucleus BY4742 [00-12] C 9 YJR077C MIR1 PTP Mitochondrial phosphate carrier; imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-12] C 10 YJR080C AIM24 FMP26 Protein with a role in determining mitochondrial architecture; inner membrane protein that interacts physically and genetically with the MICOS complex and is required for its integrity mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-12] C 11 YJR084W - CSN12 Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome response to chemical, RNA splicing, conjugation, mRNA processing, signaling molecular_function chromosome BY4742 [00-12] C 12 - chromosome chromosome chromosome - - - - [00-12] D 1 YJR087W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2 biological_process molecular_function cellular_component BY4742 [00-12] D 2 YJR090C GRR1 SCF ubiquitin ligase complex subunit GRR1|SDC1|SSU2|COT2|CAT80 F-box protein component of an SCF ubiquitin-ligase complex; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCF(Grr1) complex; SCF(Grr1) acts as a ubiquitin-protein ligase directing ubiquitination of substrates such as: Gic2p, Mks1p, Mth1p, Cln1p, Cln2p and Cln3p; involved in carbon catabolite repression, glucose-dependent divalent cation transport, glucose transport, morphogenesis, and sulfite detoxification mitotic cell cycle, response to chemical, cellular response to DNA damage stimulus, proteolysis involved in cellular protein catabolic process, protein modification by small protein conjugation or removal, conjugation, regulation of cell cycle protein binding, bridging, transferase activity nucleus, cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [00-12] D 3 YJR091C JSN1 PUF1 Member of the Puf family of RNA-binding proteins; interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl; JSN1 has a paralog, PUF2, that arose from the whole genome duplication RNA catabolic process RNA binding, mRNA binding mitochondrion, cell wall, membrane, mitochondrial envelope, cytoplasm BY4742 [00-12] D 4 - mitochondrion, cell wall, membrane, mitochondrial envelope, cytoplasm mitochondrion, cell wall, membrane, mitochondrial envelope, cytoplasm mitochondrion, cell wall, membrane, mitochondrial envelope, cytoplasm - - - - [00-12] D 5 YJR094C IME1 transcription factor IME1 Master regulator of meiosis that is active only during meiotic events; activates transcription of early meiotic genes through interaction with Ume6p; degraded by the 26S proteasome following phosphorylation by Ime2p; transcription is negatively regulated in cis by the IRT1 long noncoding antisense RNA meiotic cell cycle, organelle fission, regulation of organelle organization, pseudohyphal growth, transcription from RNA polymerase II promoter, regulation of cell cycle transcription factor activity, protein binding nucleus BY4742 [00-12] D 6 YJR094W-A RPL43B eL43|ribosomal 60S subunit protein L43B|L43e|L43B Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-12] D 7 YJR095W SFC1 ACR1 Mitochondrial succinate-fumarate transporter; transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization ion transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-12] D 8 YJR096W - aldo-keto reductase superfamily protein Xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS carbohydrate metabolic process, response to chemical, response to oxidative stress oxidoreductase activity nucleus, cytoplasm BY4742 [00-12] D 9 YJR097W JJJ3 DPH4 Protein of unknown function; contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 peptidyl-amino acid modification ion binding nucleus, cytoplasm BY4742 [00-12] D 10 YJR098C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-12] D 11 YJR099W YUH1 ubiquitin-specific protease YUH1 Ubiquitin C-terminal hydrolase; cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p other peptidase activity, hydrolase activity cytoplasm BY4742 [00-12] D 12 YJR100C AIM25 SLM35 Mitochondria protein of unknown function; interacts genetically with TOR1 to regulate chronological lifespan, and the response to both heat shock and oxidative stress; involved in maintaining the integrity of the mitochondrial network; negative regulator of mitophagy flux; non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant is viable and displays an elevated frequency of mitochondrial genome loss; similar to murine NOR1 response to heat, response to chemical, mitochondrion organization, regulation of organelle organization, response to oxidative stress molecular_function mitochondrion, cytoplasm BY4742 [00-12] E 1 YJR104C SOD1 superoxide dismutase SOD1|CRS4 Cytosolic copper-zinc superoxide dismutase; detoxifies superoxide; stabilizes Yck1p and Yck2p kinases in glucose to repress respiration; phosphorylated by Dun1p, enters nucleus under oxidative stress to promote transcription of stress response genes; human ortholog SOD1 implicated in ALS complements a null allele; abundance increases under DNA replication stress and during exposure to boric acid; localization to mitochondrial intermembrane space is modulated by MICOS complex response to chemical, generation of precursor metabolites and energy, response to oxidative stress, cellular respiration, cellular ion homeostasis, cell wall organization or biogenesis, transcription from RNA polymerase II promoter oxidoreductase activity nucleus, mitochondrion, mitochondrial envelope, cytoplasm BY4742 [00-12] E 2 YJR106W ECM27 - Protein involved in calcium homeostasis and exit from quiescence; required for proper trehalose levels during quiescence; may play a role in cell wall biosynthesis, mutants are hypersensitive to antifungal, Papulacandin B; null mutants have increased plasmid loss; interacts with Pdr5p cellular ion homeostasis, regulation of cell cycle molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-12] E 3 YJR107W LIH1 putative lipase Putative lipase biological_process hydrolase activity cellular_component BY4742 [00-12] E 4 YJR109C CPA2 carbamoyl-phosphate synthase (glutamine-hydrolyzing) CPA2 Large subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor cellular amino acid metabolic process ligase activity cytoplasm BY4742 [00-12] E 5 - cytoplasm cytoplasm cytoplasm - - - - [00-12] E 6 YJR113C RSM7 mitochondrial 37S ribosomal protein RSM7 Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S7 ribosomal protein mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-12] E 7 YJR116W TDA4 - Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-12] E 8 YJR117W STE24 zinc metalloprotease|PIO2|AFC1 Highly conserved zinc metalloprotease; functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; cleaves both isoprenylated and non-prenylated oligopeptides; contains multiple transmembrane spans; human homolog ZMPSTE24 implicated in mandibuloacral dysplasia (MAD), and can complement yeast null mutant protein maturation, conjugation peptidase activity, hydrolase activity nucleus, endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm BY4742 [00-12] E 9 YJR118C ILM1 - Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth mitochondrion organization molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-12] E 10 YJR119C JHD2 histone demethylase|KDM5 JmjC domain family histone demethylase; promotes global demethylation of H3K4 and repression of noncoding intergenic transcription during sporulation; removes methyl groups added by Set1p methyltransferase; negatively regulated by H3K14 acetylation; protein levels regulated by Not4p polyubiquitin-mediated degradation; regulates sporulation timing by extending period of active transcription in opposition to programmed global transcriptional quiescence; regulates rDNA silencing RNA catabolic process, chromatin organization, regulation of organelle organization, transcription from RNA polymerase II promoter, histone modification oxidoreductase activity nucleus, cytoplasm BY4742 [00-12] E 11 YJR120W - - Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p lipid transport, mitochondrion organization, generation of precursor metabolites and energy, cellular respiration molecular_function cellular_component BY4742 [00-12] E 12 YJR121W ATP2 F1F0 ATP synthase subunit beta Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated transmembrane transport, ion transport, nucleobase-containing small molecule metabolic process hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-12] F 1 YJR122W IBA57 CAF17 Protein involved in incorporating iron-sulfur clusters into proteins; mitochondrial matrix protein; involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system cofactor metabolic process molecular_function mitochondrion, cytoplasm BY4742 [00-12] F 2 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-12] F 3 YJR124C - - Putative protein of unknown function; expression induced under calcium shortage biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-12] F 4 YJR125C ENT3 - Protein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p cytoskeleton organization, Golgi vesicle transport, endosomal transport lipid binding, ion binding cell cortex, cytoskeleton, cytoplasmic vesicle, endomembrane system, membrane, cytoplasm BY4742 [00-12] F 5 YJR126C VPS70 putative zinc metalloprotease Protein of unknown function involved in vacuolar protein sorting; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum protein targeting molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-12] F 6 YJR131W MNS1 mannosyl-oligosaccharide 1,2-alpha-mannosidase Alpha-1,2-mannosidase; involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation response to chemical, proteolysis involved in cellular protein catabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-12] F 7 YJR133W XPT1 xanthine phosphoribosyltransferase Xanthine-guanine phosphoribosyl transferase; required for xanthine utilization and for optimal utilization of guanine nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups, transferase activity cytoplasm BY4742 [00-12] F 8 YJR134C SGM1 - Protein of unknown function; required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus biological_process molecular_function cytoplasmic vesicle, endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-12] F 9 YJR139C HOM6 homoserine dehydrogenase|THR6 Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase); dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions cellular amino acid metabolic process oxidoreductase activity nucleus, cytoplasm BY4742 [00-12] F 10 YJR140C HIR3 HPC1 Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein peptidyl-amino acid modification, mitotic cell cycle, protein acylation, chromatin organization, histone modification, regulation of protein modification process, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation chromatin binding, DNA binding, transcription factor activity, protein binding nucleus BY4742 [00-12] F 11 - nucleus nucleus nucleus - - - - [00-12] F 12 YJR142W - - 8-oxo-dGTP diphosphatase of the Nudix hydrolase family; converts diphosphates of damaged forms of thiamin to monophosphates; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition biological_process hydrolase activity cellular_component BY4742 [00-12] G 1 YJR144W MGM101 MGM9 Protein with a role in mitochondrial DNA recombinational repair; also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage and mitochondrial genome maintenance DNA repair, mitochondrion organization, DNA recombination, cellular response to DNA damage stimulus DNA binding mitochondrion, cytoplasm BY4742 [00-12] G 2 YJR145C RPS4A eS4|ribosomal 40S subunit protein S4A|S4e|rp5|YS6|S7A|S4A Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-12] G 3 YJR148W BAT2 branched-chain-amino-acid transaminase BAT2|ECA40|TWT2 Cytosolic branched-chain amino acid (BCAA) aminotransferase; preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase; BAT2 has a paralog, BAT1, that arose from the whole genome duplication cellular amino acid metabolic process transferase activity nucleus, cytoplasm BY4742 [00-12] G 4 YJR150C DAN1 CCW13 Cell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth lipid transport molecular_function cell wall BY4742 [00-12] G 5 YJR153W PGU1 PSM1|PGL1 Endo-polygalacturonase; pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins carbohydrate metabolic process, pseudohyphal growth hydrolase activity, hydrolase activity, acting on glycosyl bonds extracellular region BY4742 [00-12] G 6 YKL005C BYE1 - Negative regulator of transcription elongation; contains a TFIIS-like domain that associates with chromatin and a PHD domain that interacts with H3K4me3; multicopy suppressor of temperature-sensitive ess1 mutations, binds RNA polymerase II large subunit transcription from RNA polymerase II promoter chromatin binding, histone binding nucleus BY4742 [00-12] G 7 YKL030W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1 biological_process molecular_function membrane BY4742 [00-12] G 8 - membrane membrane membrane - - - - [00-12] G 9 YLR146C SPE4 spermine synthase Spermine synthase; required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid cofactor metabolic process, vitamin metabolic process, monocarboxylic acid metabolic process transferase activity cytoplasm BY4742 [00-12] G 11 YLR286C CTS1 SCW2 Endochitinase; required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p other hydrolase activity, hydrolase activity, acting on glycosyl bonds nucleus, endomembrane system, vacuole, cell wall, cellular bud, extracellular region, site of polarized growth, endoplasmic reticulum, cytoplasm BY4742 [00-12] G 10 - nucleus, endomembrane system, vacuole, cell wall, cellular bud, extracellular region, site of polarized growth, endoplasmic reticulum, cytoplasm nucleus, endomembrane system, vacuole, cell wall, cellular bud, extracellular region, site of polarized growth, endoplasmic reticulum, cytoplasm nucleus, endomembrane system, vacuole, cell wall, cellular bud, extracellular region, site of polarized growth, endoplasmic reticulum, cytoplasm - - - - [00-12] G 12 YLR343W GAS2 1,3-beta-glucanosyltransferase 1,3-beta-glucanosyltransferase; involved with Gas4p in spore wall assembly; has similarity to Gas1p meiotic cell cycle, sporulation, cell wall organization or biogenesis transferase activity membrane, vacuole, cytoplasm BY4742 [00-12] H 1 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [00-12] H 2 - - - - - - - - [00-12] H 3 - - - - - - - - [00-12] H 4 YML067C ERV41 - Protein localized to COPII-coated vesicles; forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Golgi vesicle transport molecular_function membrane, cytoplasmic vesicle, endomembrane system, endoplasmic reticulum, cytoplasm, Golgi apparatus BY4742 [00-12] H 5 YML068W ITT1 - Protein that modulates the efficiency of translation termination; interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins regulation of translation molecular_function cellular_component BY4742 [00-12] H 6 YML072C TCB3 - Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact lipid metabolic process lipid binding cell cortex, endomembrane system, mitochondrion, cellular bud, endoplasmic reticulum, cytoplasm BY4742 [00-12] H 7 - cell cortex, endomembrane system, mitochondrion, cellular bud, endoplasmic reticulum, cytoplasm cell cortex, endomembrane system, mitochondrion, cellular bud, endoplasmic reticulum, cytoplasm cell cortex, endomembrane system, mitochondrion, cellular bud, endoplasmic reticulum, cytoplasm - - - - [00-12] H 8 YMR136W GAT2 - Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity nucleus BY4742 [00-12] H 9 YMR172W HOT1 - Transcription factor for glycerol biosynthetic genes; required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p DNA-templated transcription, initiation, transcription from RNA polymerase II promoter, response to osmotic stress nucleic acid binding transcription factor activity nucleus BY4742 [00-12] H 10 - nucleus nucleus nucleus - - - - [00-12] H 11 YOR300W - HUF1 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 mitotic cell cycle, cytokinesis molecular_function cellular_component BY4742 [00-12] H 12 YOR309C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 biological_process molecular_function cellular_component BY4742 [00-13] A 1 - cellular_component cellular_component cellular_component - - - - [00-13] A 2 - - - - - - - - [00-13] A 3 YJL003W COX16 - Mitochondrial inner membrane protein; required for assembly of cytochrome c oxidase mitochondrion organization, protein complex biogenesis molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-13] A 4 YJL004C SYS1 - Integral membrane protein of the Golgi; required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation protein targeting, vesicle organization, Golgi vesicle transport, endosomal transport molecular_function endomembrane system, membrane, Golgi apparatus, cytoplasm BY4742 [00-13] A 5 - endomembrane system, membrane, Golgi apparatus, cytoplasm endomembrane system, membrane, Golgi apparatus, cytoplasm endomembrane system, membrane, Golgi apparatus, cytoplasm - - - - [00-13] A 6 YJL006C CTK2 - Beta subunit of C-terminal domain kinase I (CTDK-I); which phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; relocalizes to the cytosol in response to hypoxia transcription from RNA polymerase I promoter, regulation of translation, protein phosphorylation, translational elongation, DNA-templated transcription, elongation molecular_function nucleus, nucleolus, cytoplasm BY4742 [00-13] A 7 - nucleus, nucleolus, cytoplasm nucleus, nucleolus, cytoplasm nucleus, nucleolus, cytoplasm - - - - [00-13] A 8 - - - - - - - - [00-13] A 9 - - - - - - - - [00-13] A 10 - - - - - - - - [00-13] A 11 - - - - - - - - [00-13] A 12 YJL012C VTC4 YJL012C-A|PHM3 Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) complex involved in synthesis and transfer of polyP to the vacuole; regulates membrane trafficking; role in non-autophagic vacuolar fusion; protein abundance increases in response to DNA replication stress vacuole organization, organelle fusion, membrane fusion, signaling kinase activity, transferase activity, ion binding endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-13] B 1 YJL013C MAD3 - Subunit of spindle-assembly checkpoint complex; involved in delaying anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover; MAD3 has a paralog, BUB1, that arose from the whole genome duplication mitotic cell cycle, meiotic cell cycle, organelle fission, regulation of organelle organization, regulation of cell cycle, chromosome segregation molecular_function nucleus BY4742 [00-13] B 2 - nucleus nucleus nucleus - - - - [00-13] B 3 - - - - - - - - [00-13] B 4 YJL016W TPH3 YJL017W Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; contains two adjacent PH-like domains; conserved in closely related Saccharomyces species biological_process molecular_function cytoplasm BY4742 [00-13] B 5 YJL017W cytoplasm cytoplasm cytoplasm - - - BY4742 [00-13] B 6 - - - - - - - - [00-13] B 7 - - - - - - - - [00-13] B 8 YJL020C BBC1 YJL021C|MTI1 Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches cytoskeleton organization cytoskeletal protein binding cell cortex, cytoskeleton, cytoplasm BY4742 [00-13] B 9 YJL021C cell cortex, cytoskeleton, cytoplasm cell cortex, cytoskeleton, cytoplasm cell cortex, cytoskeleton, cytoplasm - - - BY4742 [00-13] B 10 YJL022W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 biological_process molecular_function cellular_component BY4742 [00-13] B 11 YJL023C PET130 - Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-13] B 12 YJL024C APS3 YKS7 Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Golgi vesicle transport, protein targeting molecular_function membrane, cytoplasm BY4742 [00-13] C 1 - membrane, cytoplasm membrane, cytoplasm membrane, cytoplasm - - - - [00-13] C 2 - - - - - - - - [00-13] C 3 YJL027C - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-13] C 4 YJL028W - - Protein of unknown function; may interact with ribosomes, based on co-purification experiments biological_process molecular_function ribosome, cytoplasm BY4742 [00-13] C 5 YJL029C VPS53 - Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; required for vacuolar protein sorting; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p; human ortholog is implicated in progressive cerebello-cerebral atrophy type 2 (PCCA2) Golgi vesicle transport, endosomal transport molecular_function cytoplasmic vesicle, endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-13] C 6 YJR001W AVT1 - Vacuolar transporter; imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters transmembrane transport, ion transport, amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [00-13] C 7 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [00-13] C 8 - - - - - - - - [00-13] C 9 YJR004C SAG1 AG(ALPHA)1 Alpha-agglutinin of alpha-cells; binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor response to chemical, conjugation other cell wall, vacuole, cytoplasm BY4742 [00-13] C 10 YJR005W APL1 YAP80 Beta-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex other molecular_function cytoplasmic vesicle, endomembrane system, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane BY4742 [00-13] C 11 - cytoplasmic vesicle, endomembrane system, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane cytoplasmic vesicle, endomembrane system, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane cytoplasmic vesicle, endomembrane system, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane - - - - [00-13] C 12 - - - - - - - - [00-13] D 1 YJR008W MHO1 - Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility biological_process molecular_function nucleus, cytoplasm BY4742 [00-13] D 2 YJR009C TDH2 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) TDH2|GAPDH|GLD2 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; GAPDH-derived antimicrobial peptides are active against a wide variety of wine-related yeasts and bateria; TDH2 has a paralog, TDH3, that arose from the whole genome duplication carbohydrate metabolic process, cofactor metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, nucleobase-containing small molecule metabolic process chromatin binding, oxidoreductase activity nucleus, mitochondrion, cell wall, membrane, cytoplasm, plasma membrane BY4742 [00-13] D 3 YJR010C-A SPC1 signal peptidase complex subunit SPC1 Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress protein targeting, protein maturation molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-13] D 4 - membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm - - - - [00-13] D 5 YJR011C - - Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS biological_process molecular_function cellular_component BY4742 [00-13] D 6 - cellular_component cellular_component cellular_component - - - - [00-13] D 7 - - - - - - - - [00-13] D 8 YJR014W TMA22 RBF22 Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress biological_process RNA binding ribosome, cytoplasm BY4742 [00-13] D 9 YJR015W - - Putative protein of unknown function; localizes to endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; not an essential gene; YJR015W has a paralog, SNG1, that arose from the whole genome duplication biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-13] D 10 - membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm - - - - [00-13] D 11 - - - - - - - - [00-13] D 12 YJR018W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-13] E 1 YJR019C TES1 PTE1 Peroxisomal acyl-CoA thioesterase; likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids lipid metabolic process, monocarboxylic acid metabolic process hydrolase activity peroxisome, mitochondrion, cytoplasm BY4742 [00-13] E 2 YJR020W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-13] E 3 YJR021C REC107 MER2 Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation meiotic cell cycle, organelle fission, DNA recombination molecular_function nucleus, chromosome BY4742 [00-13] E 4 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [00-13] E 5 - - - - - - - - [00-13] E 6 YJR024C MDE1 methylthioribulose 1-phosphate dehydratase MDE1 5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant cellular amino acid metabolic process lyase activity cytoplasm BY4742 [00-13] E 7 YJR025C BNA1 3-hydroxyanthranilate 3,4-dioxygenase|HAD1 3-hydroxyanthranilic acid dioxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p cofactor metabolic process, nucleobase-containing small molecule metabolic process, cellular amino acid metabolic process oxidoreductase activity cytoplasm BY4742 [00-13] E 8 YJR026W cytoplasm cytoplasm cytoplasm - - - BY4742 [00-13] E 9 - - - - - - - - [00-13] E 10 - - - - - - - - [00-13] E 11 - - - - - - - - [00-13] E 12 YJR030C RBH2 RBH2 Putative protein of unknown function; expression repressed in carbon limited vs carbon replete chemostat cultures; non-essential gene; contains a PH-like domain; RBH2 has a paralog, RBH1, that arose from the whole genome duplication carbohydrate metabolic process molecular_function cellular_component BY4742 [00-13] F 1 - cellular_component cellular_component cellular_component - - - - [00-13] F 2 YJR032W CPR7 peptidylprolyl isomerase CPR7 Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; plays a role in determining prion variants response to heat, protein folding unfolded protein binding, isomerase activity cytoplasm BY4742 [00-13] F 3 YJR033C RAV1 SOI3 Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate cellular ion homeostasis, endosomal transport, protein complex biogenesis molecular_function membrane, cytoplasm BY4742 [00-13] F 4 - membrane, cytoplasm membrane, cytoplasm membrane, cytoplasm - - - - [00-13] F 5 YJR035W RAD26 DNA-dependent ATPase RAD26 Protein involved in transcription-coupled nucleotide excision repair; repairs UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein DNA repair, chromatin organization, cellular response to DNA damage stimulus ATPase activity, hydrolase activity nucleus, cytoplasm BY4742 [00-13] F 6 YJR036C HUL4 putative E3 ubiquitin-protein ligase HUL4 Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex protein modification by small protein conjugation or removal transferase activity nucleus, membrane BY4742 [00-13] F 7 YJR037W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces biological_process molecular_function cellular_component BY4742 [00-13] F 8 YJR038C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-13] F 9 - cellular_component cellular_component cellular_component - - - - [00-13] F 10 YJR040W GEF1 CLC Voltage-gated chloride channel; localized to the golgi, the endosomal system, and plasma membrane; involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism cellular ion homeostasis transmembrane transporter activity cytoplasmic vesicle, endomembrane system, vacuole, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm, plasma membrane BY4742 [00-13] F 11 - cytoplasmic vesicle, endomembrane system, vacuole, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm, plasma membrane cytoplasmic vesicle, endomembrane system, vacuole, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm, plasma membrane cytoplasmic vesicle, endomembrane system, vacuole, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm, plasma membrane - - - - [00-13] F 12 - - - - - - - - [00-13] G 1 YJR043C POL32 DNA polymerase delta subunit POL32|REV5 Third subunit of DNA polymerase delta; involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; forms a complex with Rev3p, Rev7p and Pol31p; interacts with Hys2p, PCNA (Pol30p), and Pol1p DNA repair, DNA replication, cellular response to DNA damage stimulus, RNA catabolic process, DNA recombination transferase activity, nucleotidyltransferase activity, hydrolase activity, nuclease activity nucleus, chromosome BY4742 [00-13] G 2 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [00-13] G 3 - - - - - - - - [00-13] G 4 - - - - - - - - [00-13] G 5 YJR047C ANB1 translation elongation factor eIF-5A|eIF-5A|eIF5A|TIF51B|HYP1 Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication; human EIF5A complements the inviability of the yeast hyp2 anb1 double null mutant regulation of translation, translational elongation RNA binding, translation factor activity, RNA binding ribosome, cytoplasm BY4742 [00-13] G 6 YJR048W CYC1 cytochrome c isoform 1 Cytochrome c, isoform 1; also known as iso-1-cytochrome c; electron carrier of mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication; human homolog CYC1 can complement yeast null mutant; mutations in human CYC1 cause insulin-responsive hyperglycemia nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, cellular respiration other mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-13] G 7 YJR049C UTR1 NADH/NAD(+) kinase ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication cofactor metabolic process, cellular ion homeostasis, nucleobase-containing small molecule metabolic process kinase activity, transferase activity nucleus, cytoplasm BY4742 [00-13] G 8 YJR050W ISY1 NTC30|UTR3 Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs; interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles RNA splicing, mRNA processing other nucleus BY4742 [00-13] G 9 YJR051W OSM1 fumarate reductase|FRDS2 Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; has two translation start sites, one at the annotated start codon which produces an ER-targeted form required for anaerobic growth, and one at codon 32 which produces a mitochondrially-targeted form; OSM1 has a paralog, FRD1, that arose from the whole genome duplication cofactor metabolic process, protein folding, nucleobase-containing small molecule metabolic process oxidoreductase activity endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [00-13] G 10 YJR052W RAD7 UV-damaged DNA-binding protein RAD7 Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad16p) during NER; required for repair of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex DNA repair, cellular response to DNA damage stimulus, proteolysis involved in cellular protein catabolic process, protein modification by small protein conjugation or removal transferase activity, hydrolase activity, DNA binding, ATPase activity nucleus BY4742 [00-13] G 11 YJR053W BFA1 IBD1 Subunit of a two-component GTPase-activating protein, Bfa1p-Bub2p; contributes to GAP activity, inactivating Tem1 by stimulating GTP hydrolysis following damage or misalignment of the mitotic spindle; functions as a guanine-nucleotide exchange inhibitor (GDI) for Tem1p; involved in multiple cell cycle checkpoint pathways that control mitotic exit; required when telomeres are damaged, but not for all types of chromosomal DNA damage; phosphorylated by the Polo-like kinase Cdc5p mitotic cell cycle, organelle fission, regulation of organelle organization, chromosome segregation, regulation of cell cycle enzyme regulator activity cytoskeleton, microtubule organizing center, cytoplasm BY4742 [00-13] G 12 - cytoskeleton, microtubule organizing center, cytoplasm cytoskeleton, microtubule organizing center, cytoplasm cytoskeleton, microtubule organizing center, cytoplasm - - - - [00-13] H 1 - - - - - - - - [00-13] H 2 YJR056C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus, cytoplasm BY4742 [00-13] H 3 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-13] H 4 YJR058C APS2 YAP17 Small subunit of the clathrin-associated adaptor complex AP-2; AP-2 is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex other molecular_function nucleus, cytoplasmic vesicle, endomembrane system, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane BY4742 [00-13] H 5 YJR059W PTK2 protein kinase PTK2|STK2 Serine/threonine protein kinase; involved in regulation of ion transport across plasma membrane; carboxyl terminus is essential for glucose-dependent Pma1p activation via phosphorylation of Pma1p-Ser899; enhances spermine uptake; PTK2 has a paralog, PTK1, that arose from the whole genome duplication mitotic cell cycle, protein phosphorylation, cellular ion homeostasis kinase activity, transferase activity nucleus, membrane, cytoplasm, plasma membrane BY4742 [00-13] H 6 YJR060W CBF1 GFII|CP1|CPF1|CEP1 Basic helix-loop-helix (bHLH) protein; forms homodimer to bind E-box consensus sequence CACGTG present at MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins, required for chromosome segregation; protein abundance increases in response to DNA replication stress carbohydrate metabolic process, response to chemical, chromatin organization, chromosome segregation, lipid metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding, transcription factor binding nucleus, chromosome, mitochondrion, cytoplasm BY4742 [00-13] H 7 YJR061W - - Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4742 [00-13] H 8 YJR062C NTA1 amidase|DEA1 Amidase; removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation other hydrolase activity mitochondrion, cytoplasm BY4742 [00-13] H 9 YJR063W RPA12 DNA-directed RNA polymerase I core subunit RPA12|A12.2|RRN4 RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex; physically interacts with transcriptional activator Msn4p, to regulate transcription of AYR1, a gene involved in lipid metabolism transcription from RNA polymerase I promoter, DNA-templated transcription, termination, transcription from RNA polymerase II promoter transferase activity, nucleotidyltransferase activity, transcription factor binding nucleus, nucleolus BY4742 [00-13] H 10 - nucleus, nucleolus nucleus, nucleolus nucleus, nucleolus - - - - [00-13] H 11 - - - - - - - - [00-13] H 12 - - - - - - - - [00-14] A 1 YBR189W RPS9B ribosomal 40S subunit protein S9B|rp21|YS11|S9B|S4|S13|SUP46|RPS13A Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication regulation of translation, rRNA processing, translational elongation, ribosomal small subunit biogenesis structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [00-14] A 2 YCR095C OCA4 - Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts biological_process molecular_function cytoplasm BY4742 [00-14] A 3 YCR102W-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-14] A 4 YDL133C-A RPL41B eL41|ribosomal 60S subunit protein L41B|L41e|YL41|L47B|L41B|YDL134C-A|RPL47B Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-14] A 5 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [00-14] A 6 - - - - - - - - [00-14] A 7 YDR058C TGL2 triglyceride lipase Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli lipid metabolic process hydrolase activity mitochondrion, cytoplasm BY4742 [00-14] A 8 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-14] A 9 YDR174W HMO1 HSM2 Chromatin associated high mobility group (HMG) family member; involved in compacting, bending, bridging and looping DNA; rDNA-binding component that regulates transcription from RNA polymerase I promoters; regulates start site selection of ribosomal protein genes via RNA polymerase II promoters; role in genome maintenance; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase; relocalizes to the cytosol in response to hypoxia transcription from RNA polymerase I promoter, DNA-templated transcription, initiation, transcription from RNA polymerase II promoter DNA binding nucleus, nucleolus, chromosome, cytoplasm BY4742 [00-14] A 10 YDR202C RAV2 - Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme cellular ion homeostasis, protein complex biogenesis molecular_function cytoplasm BY4742 [00-14] A 11 YDR205W MSC2 metal cation transporter MSC2 Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids transmembrane transport, ion transport, cellular ion homeostasis transmembrane transporter activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-14] A 12 YDR445C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-14] B 1 YDR537C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps verified ORF PAD1/YDR538W biological_process molecular_function cellular_component BY4742 [00-14] B 2 - cellular_component cellular_component cellular_component - - - - [00-14] B 3 YFR039C OSW7 - Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; may be involved in response to high salt and changes in carbon source; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; deletion mutant has decreased spore survival in Drosophila feces; OSW7 has a paralog, SHE10, that arose from the whole genome duplication; paralogs are redundant for spore wall dityrosine assembly meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-14] B 4 YGL219C MDM34 ERMES complex subunit MDM34|MMM2 Mitochondrial component of the ERMES complex; links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase mitochondrion organization, ion transport, lipid transport molecular_function endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm BY4742 [00-14] B 5 - endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm - - - - [00-14] B 6 YGR028W MSP1 YTA4 Highly-conserved N-terminally anchored AAA-ATPase; distributed in the mitochondrial outer membrane and peroxisomes; involved in mitochondrial protein sorting; functions as an extraction engine in local organelle surveillance to remove and initiate degradation of mistargeted proteins, ensuring fidelity of organelle-specific localization of tail-anchored proteins; contains an N-terminal transmembrane domain and C-terminal cytoplasmic ATPase domain protein targeting, mitochondrion organization hydrolase activity, ATPase activity peroxisome, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-14] B 7 YGR032W GSC2 1,3-beta-glucan synthase GSC2|FKS2 Catalytic subunit of 1,3-beta-glucan synthase; involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; GSC2 has a paralog, FKS1, that arose from the whole genome duplication meiotic cell cycle, carbohydrate metabolic process, sporulation, cell wall organization or biogenesis transferase activity, transferring glycosyl groups, transferase activity cellular bud, membrane, site of polarized growth, plasma membrane BY4742 [00-14] B 8 YGR038W ORM1 sphingolipid homeostasis protein ORM1 Protein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; ORM1 has a paralog, ORM2, that arose from the whole genome duplication response to chemical, lipid metabolic process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-14] B 9 YGR040W KSS1 mitogen-activated serine/threonine-protein kinase KSS1 Mitogen-activated protein kinase (MAPK); involved in signal transduction pathways that control filamentous growth and pheromone response; regulates septum assembly, and may directly phosphorylate Bni4p; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains mitotic cell cycle, response to chemical, protein phosphorylation, invasive growth in response to glucose limitation, cytokinesis, conjugation, signaling, regulation of cell cycle kinase activity, transferase activity, signal transducer activity, transcription factor binding nucleus, cellular bud, site of polarized growth BY4742 [00-14] B 10 - nucleus, cellular bud, site of polarized growth nucleus, cellular bud, site of polarized growth nucleus, cellular bud, site of polarized growth - - - - [00-14] B 11 - - - - - - - - [00-14] B 12 YGR050C - - Protein of unknown function; mRNA identified as translated by ribosome profiling data biological_process molecular_function cellular_component BY4742 [00-14] C 1 YGR053C - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [00-14] C 2 YGR063C SPT4 transcription elongation factor SPT4 Spt4p/5p (DSIF) transcription elongation factor complex subunit; the Spt4/5 complex binds to ssRNA in a sequence-specific manner, and along with RNAP I and II has multiple roles regulating transcriptional elongation, RNA processing, quality control, and transcription-coupled repair; localizes to kinetochores and heterochromatin, influencing chromosomal dynamics and silencing; required for transcription through long trinucleotide repeats in ORFs and non-protein coding regions transcription from RNA polymerase I promoter, chromatin organization, mRNA processing, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation, DNA repair, RNA splicing, rRNA processing, cellular response to DNA damage stimulus, chromosome segregation, regulation of DNA metabolic process RNA binding, enzyme binding, DNA binding, transcription factor activity, protein binding nucleus, chromosome BY4742 [00-14] C 3 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [00-14] C 4 YGR086C PIL1 lipid-binding protein PIL1 Eisosome core component; eisosomes are large immobile cell cortex structures associated with endocytosis; detected in phosphorylated state in mitochondria; phosphorylated on Thr233 upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; member of BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress response to heat, protein phosphorylation, regulation of protein modification process, endocytosis lipid binding mitochondrion, membrane, mitochondrial envelope, cytoplasm, plasma membrane BY4742 [00-14] C 5 YGR089W NNF2 - Protein that exhibits physical and genetic interactions with Rpb8p; Rpb8p is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-14] C 6 YGR092W DBF2 serine/threonine-protein kinase DBF2 Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis; DBF2 has a paralog, DBF20, that arose from the whole genome duplication protein phosphorylation, organelle fission, cytokinesis, cellular ion homeostasis, regulation of cell cycle kinase activity, transferase activity cytoskeleton, microtubule organizing center, cellular bud, site of polarized growth BY4742 [00-14] C 7 YGR093W DRN1 - Splicing factor that modulates turnover of branched RNAs by Dbr1p; interacts with spliceosomal components and branched RNA splicing products; enhances Dbr1p debranching in vitro; conserved protein with domain organization identical from yeast to human; N-terminal homology to Dbr1p N-terminus, but Dbr1p catalytic residues not conserved; relocalizes to the cytosol in response to hypoxia RNA splicing, mRNA processing enzyme regulator activity nucleus, cytoplasm BY4742 [00-14] C 8 YGR106C VOA1 - ER protein that functions in assembly of the V0 sector of V-ATPase; functions with other assembly factors; null mutation enhances the vacuolar ATPase (V-ATPase) deficiency of a vma21 mutant impaired in endoplasmic reticulum (ER) retrieval protein complex biogenesis molecular_function membrane, endomembrane system, vacuole, endoplasmic reticulum, cytoplasm BY4742 [00-14] C 9 YGR110W CLD1 carboxylic ester hydrolase Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 lipid metabolic process hydrolase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-14] C 10 - membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm - - - - [00-14] C 11 YGR117C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm BY4742 [00-14] C 12 YGR238C KEL2 - Protein that negatively regulates mitotic exit; forms a complex with Kel1p and Bud14p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; functions in a complex with Kel1p, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, cytokinesis, regulation of transport, conjugation, regulation of cell cycle molecular_function cellular bud, site of polarized growth BY4742 [00-14] D 1 YGR239C PEX21 - Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; constitutively expressed; partially redundant with Pex18p; required for import of the Gpd1p-Pnc1p heterodimer in which only Gpd1p has a peroxisomal targeting signal; relative distribution to cytoplasmic foci increases upon DNA replication stress protein targeting, transmembrane transport, peroxisome organization other peroxisome, cytoplasm BY4742 [00-14] D 2 YGR248W SOL4 6-phosphogluconolactonase SOL4 6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication cofactor metabolic process, nucleobase-containing small molecule metabolic process hydrolase activity nucleus, cytoplasm BY4742 [00-14] D 3 YGR250C RIE1 - Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress biological_process RNA binding, mRNA binding cytoplasm BY4742 [00-14] D 4 YJL129C TRK1 - Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication cellular ion homeostasis transmembrane transporter activity membrane, plasma membrane BY4742 [00-14] D 5 YJL132W - - Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-14] D 6 YJL136C RPS21B eS21|ribosomal 40S subunit protein S21B|S21e|YS25|S26B|S21B Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-14] D 7 YJL137C GLG2 glycogenin glucosyltransferase GLG2 Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication carbohydrate metabolic process, generation of precursor metabolites and energy transferase activity, transferring glycosyl groups, transferase activity cytoplasm BY4742 [00-14] D 8 YJL139C YUR1 mannosyltransferase YUR1 Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication protein glycosylation, cell wall organization or biogenesis transferase activity, transferring glycosyl groups, transferase activity endomembrane system, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4742 [00-14] D 9 YJL140W RPB4 DNA-directed RNA polymerase II subunit RPB4|B32|CTF15 RNA polymerase II subunit B32; forms dissociable heterodimer with Rpb7p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNAPII complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation response to heat, translational initiation, regulation of translation, nucleobase-containing compound transport, RNA catabolic process, DNA-templated transcription, initiation, mRNA processing, transcription from RNA polymerase II promoter, nuclear transport RNA binding, transferase activity, nucleotidyltransferase activity, DNA binding nucleus, cytoplasm BY4742 [00-14] D 10 YJL141C YAK1 serine/threonine protein kinase YAK1 Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose protein phosphorylation kinase activity, transferase activity nucleus, cytoplasm BY4742 [00-14] D 11 YJL151C SNA3 - Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles endosomal transport enzyme binding membrane, vacuole, cytoplasm BY4742 [00-14] D 12 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [00-14] E 1 YJL160C PIR5 beta-1,3-glucan linked protein Putative protein of unknown function; member of the PIR (Proteins with Internal Repeats) family of cell wall proteins; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, and mCherry fusion localizes to the vacuole; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication biological_process molecular_function vacuole, cytoplasm BY4742 [00-14] E 2 YJL161W FMP33 - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-14] E 3 YJL163C - - Putative protein of unknown function biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-14] E 4 YJL165C HAL5 protein kinase HAL5 Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication cellular ion homeostasis, protein phosphorylation, protein dephosphorylation kinase activity, transferase activity cellular_component BY4742 [00-14] E 5 YJL172W CPS1 Gly-Xaa carboxypeptidase Vacuolar carboxypeptidase S; expression is induced under low-nitrogen conditions proteolysis involved in cellular protein catabolic process peptidase activity, hydrolase activity vacuole, cytoplasm BY4742 [00-14] E 6 - vacuole, cytoplasm vacuole, cytoplasm vacuole, cytoplasm - - - - [00-14] E 7 YJL175W - - Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor biological_process molecular_function membrane BY4742 [00-14] E 8 YJL177W RPL17B uL22|ribosomal 60S subunit protein L17B|L22|YL17|L20B|L17B Ribosomal 60S subunit protein L17B; required for processing of 27SB pre-rRNA and formation of stable 66S assembly intermediates; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal large subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-14] E 9 YJL184W GON7 chromatin DNA-binding EKC/KEOPS complex subunit GON7|PCC2|LDB6 Component of the EKC/KEOPS protein complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; implicated in osmotic stress response; other complex members are Kae1p, Cgi121p, Pcc1p, and Bud32p telomere organization, DNA recombination, cell wall organization or biogenesis, transcription from RNA polymerase II promoter chromatin binding, DNA binding nucleus, chromosome BY4742 [00-14] E 10 YJL189W RPL39 eL39|ribosomal 60S subunit protein L39|L39e|YL40|L46|L39|SPB2|RPL46 Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-14] E 11 YJL191W RPS14B uS11|ribosomal 40S subunit protein S14B|S11|rp59B|S14B|CRY2 Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly RNA binding, structural constituent of ribosome, rRNA binding, structural molecule activity ribosome, cytoplasm BY4742 [00-14] E 12 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [00-14] F 1 YJL196C ELO1 fatty acid elongase ELO1 Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, ELO2, that arose from the whole genome duplication lipid metabolic process, monocarboxylic acid metabolic process transferase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-14] F 2 YJL200C ACO2 aconitate hydratase ACO2 Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol biological_process lyase activity mitochondrion, cytoplasm BY4742 [00-14] F 3 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-14] F 4 YJL206C - - Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment biological_process molecular_function cellular_component BY4742 [00-14] F 5 YJL213W - - Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p biological_process molecular_function ribosome, cytoplasm BY4742 [00-14] F 6 YKL096W-A CWP2 YKL097W-A|LPR1 Covalently linked cell wall mannoprotein; major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored cell wall organization or biogenesis structural molecule activity cell wall, cytoplasm BY4742 [00-14] F 7 YKL115C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRR1 biological_process molecular_function cellular_component BY4742 [00-14] F 8 YKL139W CTK1 cyclin-dependent serine/threonine protein kinase CTK1 Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; required for H3K36 trimethylation but not dimethylation by Set2p; suggested stimulatory role in 80S formation during translation initiation; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 transcription from RNA polymerase I promoter, peptidyl-amino acid modification, translational initiation, regulation of translation, protein phosphorylation, translational elongation, mRNA processing, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation kinase activity, transferase activity nucleus, nucleolus BY4742 [00-14] F 9 - nucleus, nucleolus nucleus, nucleolus nucleus, nucleolus - - - - [00-14] F 10 - - - - - - - - [00-14] F 11 - - - - - - - - [00-14] F 12 YKL194C MST1 threonine--tRNA ligase MST1 Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(UAG)Q2 in vitro; lacks a typical editing domain, but has pre-transfer editing activity stimulated by the unusual tRNA-Thr mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm BY4742 [00-14] G 1 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-14] G 2 YKL201C MNN4 YKL200C Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; coding sequence contains length polymorphisms in different strains; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication protein glycosylation, response to osmotic stress enzyme regulator activity membrane BY4742 [00-14] G 3 YKL202W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-14] G 4 YKL204W EAP1 - eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade RNA catabolic process, regulation of translation RNA binding, mRNA binding cytoplasm BY4742 [00-14] G 5 - cytoplasm cytoplasm cytoplasm - - - - [00-14] G 6 YKL215C OXP1 5-oxoprolinase 5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress other hydrolase activity cytoplasm BY4742 [00-14] G 7 YKL220C FRE2 ferric/cupric-chelate reductase Ferric reductase and cupric reductase; reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels ion transport oxidoreductase activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [00-14] G 8 - membrane, vacuole, plasma membrane, cytoplasm membrane, vacuole, plasma membrane, cytoplasm membrane, vacuole, plasma membrane, cytoplasm - - - - [00-14] G 9 YKR010C TOF2 - Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication chromatin organization, nucleus organization enzyme regulator activity nucleus, nucleolus, mitochondrion, cytoplasm BY4742 [00-14] G 10 YKR019C IRS4 - EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication chromatin organization, response to starvation, cell wall organization or biogenesis, signaling molecular_function mitochondrion, cytoplasm BY4742 [00-14] G 11 YKR023W - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-14] G 12 YKR027W BCH2 FMP50 Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication carbohydrate metabolic process, Golgi vesicle transport, cell wall organization or biogenesis molecular_function cytoplasmic vesicle, endomembrane system, mitochondrion, Golgi apparatus, cytoplasm BY4742 [00-14] H 1 YKR028W SAP190 - Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication mitotic cell cycle, tRNA processing, signaling, RNA modification molecular_function cytoplasm BY4742 [00-14] H 2 YKR029C SET3 histone-binding protein SET3 Defining member of the SET3 histone deacetylase complex; which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication meiotic cell cycle, organelle fission, chromatin organization, regulation of organelle organization, histone modification, regulation of cell cycle histone binding nucleus, chromosome BY4742 [00-14] H 3 YKR034W DAL80 UGA43 Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [00-14] H 4 YKR036C CAF4 - WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication organelle fission, mitochondrion organization, transcription from RNA polymerase II promoter, peroxisome organization molecular_function mitochondrion, cytoplasm BY4742 [00-14] H 5 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-14] H 6 YKR039W GAP1 amino acid permease GAP1 General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth ion transport, amino acid transport transmembrane transporter activity cytoplasmic vesicle, endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [00-14] H 7 YKR040C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W biological_process molecular_function membrane BY4742 [00-14] H 8 YKR041W - - Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking biological_process molecular_function nucleus, cytoskeleton, cytoplasm BY4742 [00-14] H 9 YKR046C PET10 - Protein of unknown function that localizes to lipid particles; localization suggests a role in lipid metabolism; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange generation of precursor metabolites and energy, cellular respiration molecular_function other BY4742 [00-14] H 10 YKR053C YSR3 sphinganine kinase YSR3|LBP2 Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication lipid metabolic process phosphatase activity, hydrolase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-14] H 11 YML035C AMD1 AMP deaminase|AMD3 AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools nucleobase-containing small molecule metabolic process hydrolase activity cytoplasm BY4742 [00-14] H 12 - cytoplasm cytoplasm cytoplasm - - - - [00-15] A 1 - - - - - - - - [00-15] A 2 - - - - - - - - [00-15] A 3 - - - - - - - - [00-15] A 4 - - - - - - - - [00-15] A 5 - - - - - - - - [00-15] A 6 - - - - - - - - [00-15] A 7 - - - - - - - - [00-15] A 8 - - - - - - - - [00-15] A 9 - - - - - - - - [00-15] A 10 - - - - - - - - [00-15] A 11 - - - - - - - - [00-15] A 12 - - - - - - - - [00-15] B 1 YFL013W-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component BY4742 [00-15] B 2 YFL014W HSP12 lipid-binding protein HSP12|HOR5|GLP1 Plasma membrane protein involved in maintaining membrane organization; involved in maintaining organization during stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension response to heat, response to chemical, response to oxidative stress, response to osmotic stress lipid binding nucleus, cytoplasmic vesicle, endomembrane system, membrane, cytoplasm, plasma membrane BY4742 [00-15] B 3 - nucleus, cytoplasmic vesicle, endomembrane system, membrane, cytoplasm, plasma membrane nucleus, cytoplasmic vesicle, endomembrane system, membrane, cytoplasm, plasma membrane nucleus, cytoplasmic vesicle, endomembrane system, membrane, cytoplasm, plasma membrane - - - - [00-15] B 4 YFL019C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YFL019C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-15] B 5 - cellular_component cellular_component cellular_component - - - - [00-15] B 6 - - - - - - - - [00-15] B 7 - - - - - - - - [00-15] B 8 YFL042C LAM5 LTC2|YFL043C Putative sterol transfer protein; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; has both GRAM and StART-like (VASt) domains; localizes to membrane contact sites throughout the cell, including nucleus-vacuole junctions and ER-mitochondrial contact sites lipid transport other endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-15] B 9 - endomembrane system, endoplasmic reticulum, cytoplasm endomembrane system, endoplasmic reticulum, cytoplasm endomembrane system, endoplasmic reticulum, cytoplasm - - - - [00-15] B 10 - - - - - - - - [00-15] B 11 - - - - - - - - [00-15] B 12 YFR019W FAB1 1-phosphatidylinositol-3-phosphate 5-kinase|SVL7 1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis lipid metabolic process kinase activity, transferase activity, lipid binding, ion binding cytoplasmic vesicle, endomembrane system, vacuole, mitochondrion, membrane, cytoplasm BY4742 [00-15] C 1 YFR024C cytoplasmic vesicle, endomembrane system, vacuole, mitochondrion, membrane, cytoplasm cytoplasmic vesicle, endomembrane system, vacuole, mitochondrion, membrane, cytoplasm cytoplasmic vesicle, endomembrane system, vacuole, mitochondrion, membrane, cytoplasm - - - BY4742 [00-15] C 2 YFR025C HIS2 histidinol-phosphatase Histidinolphosphatase; catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control cellular amino acid metabolic process phosphatase activity, hydrolase activity cytoplasm BY4742 [00-15] C 3 - cytoplasm cytoplasm cytoplasm - - - - [00-15] C 4 - - - - - - - - [00-15] C 5 YFR030W MET10 sulfite reductase subunit alpha Subunit alpha of assimilatory sulfite reductase; complex converts sulfite into sulfide other oxidoreductase activity other BY4742 [00-15] C 6 - other other other - - - - [00-15] C 7 - - - - - - - - [00-15] C 8 - - - - - - - - [00-15] C 9 YHR146W CRP1 - Protein that binds to cruciform DNA structures; CRP1 has a paralog, MDG1, that arose from the whole genome duplication biological_process DNA binding nucleus BY4742 [00-15] C 10 YHR171W ATG7 APG11|APG7|CVT2 Autophagy-related protein and dual specificity member of the E1 family; mediates the attachment of Atg12p to Atg5p and Atg8p to phosphatidylethanolamine which are required steps in autophagosome formation; E1 enzymes are also known as ubiquitin-activating enzymes; involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner protein targeting, mitochondrion organization, protein modification by small protein conjugation or removal, protein lipidation ligase activity mitochondrion, membrane, cytoplasm BY4742 [00-15] C 11 - mitochondrion, membrane, cytoplasm mitochondrion, membrane, cytoplasm mitochondrion, membrane, cytoplasm - - - - [00-15] C 12 YJL042W MHP1 - Microtubule-associated protein involved in microtubule organization; involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins cytoskeleton organization, regulation of organelle organization, cell wall organization or biogenesis structural molecule activity cytoskeleton BY4742 [00-15] D 1 YJL070C - metallo-dependent hydrolase superfamily protein Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-15] D 2 YJL078C PRY3 - Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p lipid transport molecular_function cell wall BY4742 [00-15] D 3 YJL094C KHA1 - Putative K+/H+ antiporter; has a probable role in intracellular cation homeostasis; localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies ion transport transmembrane transporter activity endomembrane system, mitochondrion, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4742 [00-15] D 4 - endomembrane system, mitochondrion, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm endomembrane system, mitochondrion, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm endomembrane system, mitochondrion, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm - - - - [00-15] D 5 YJL101C GSH1 glutamate--cysteine ligase Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress response to chemical, response to oxidative stress ligase activity other BY4742 [00-15] D 6 - other other other - - - - [00-15] D 7 YJL105W SET4 - Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4742 [00-15] D 8 - cellular_component cellular_component cellular_component - - - - [00-15] D 9 YJL128C PBS2 mitogen-activated protein kinase kinase PBS2|SSK4|SFS4|HOG4 MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition response to heat, cytoskeleton organization, response to osmotic stress, protein targeting, protein phosphorylation, regulation of protein modification process, nuclear transport, signaling enzyme binding, signal transducer activity, kinase activity, transferase activity, structural molecule activity cellular bud, site of polarized growth, cytoplasm BY4742 [00-15] D 10 YKR085C MRPL20 mitochondrial 54S ribosomal protein YmL20|YmL20 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-15] D 11 YKR094C RPL40B eL40|ubiquitin-ribosomal 60S subunit protein L40B fusion protein|L40e|L40B|UB12|CEP52B|UBI2 Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication cytoplasmic translation, organelle assembly, ribosomal subunit export from nucleus, ribosomal large subunit biogenesis, ribosome assembly, protein modification by small protein conjugation or removal, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm BY4742 [00-15] D 12 YKR095W MLP1 MPL1 Myosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs; MLP1 has a paralog, MLP2, that arose from the whole genome duplication protein targeting, chromatin organization, nucleobase-containing compound transport, RNA catabolic process, transcription from RNA polymerase II promoter, nuclear transport, nucleus organization RNA binding, mRNA binding nucleus, cytoskeleton, microtubule organizing center, endomembrane system, membrane BY4742 [00-15] E 1 YKR096W ESL2 - hEST1A/B (SMG5/6)-like protein; contributes to environment-sensing adaptive gene expression responses; Esl2p and Esl1p contain a 14-3-3-like domain and a putative PilT N-terminus ribonuclease domain; interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; ESL2 has a paralog, ESL1, that arose from the whole genome duplication biological_process molecular_function nucleus, ribosome, cytoplasm BY4742 [00-15] E 2 YKR102W FLO10 - Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth invasive growth in response to glucose limitation other cell wall BY4742 [00-15] E 3 YLR110C CCW12 - Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication response to chemical, conjugation, cell wall organization or biogenesis structural molecule activity vacuole, cell wall, cellular bud, site of polarized growth, cytoplasm BY4742 [00-15] E 4 YLR390W-A CCW14 ICWP|YLR391W|SSR1|YLR391W-A Covalently linked cell wall glycoprotein; present in the inner layer of the cell wall cell wall organization or biogenesis structural molecule activity cell wall, mitochondrion, cytoplasm BY4742 [00-15] E 5 YLR439W MRPL4 mitochondrial 54S ribosomal protein YmL4|YmL4 Mitochondrial ribosomal protein of the large subunit; homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-15] E 6 YLR442C SIR3 chromatin-silencing protein SIR3|STE8|MAR2|CMT1 Silencing protein; interacts with Sir2p, Sir4p, and histone H3/H4 tails to establish transcriptionally silent chromatin; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p; C-terminus assumes variant winged helix-turn-helix (wH) fold that mediates homodimerization, which is critical for holo-SIR complex loading; required for telomere hypercluster formation in quiescent yeast cells; has paralog ORC1 from whole genome duplication chromatin organization, RNA catabolic process chromatin binding, histone binding, DNA binding nucleus, chromosome, mitochondrion, nucleolus, cytoplasm BY4742 [00-15] E 7 YLR455W PDP3 - Component of the NuA3b histone acetyltransferase complex; regulates interaction between NuA3b and H3K36me3 at the transcribed regions of genes; contains PWWP domain; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia transcription from RNA polymerase II promoter, DNA-templated transcription, elongation chromatin binding nucleus, cytoplasm BY4742 [00-15] E 8 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-15] E 9 YML066C SMA2 - Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function membrane, vacuole, cytoplasm BY4742 [00-15] E 10 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [00-15] E 11 - - - - - - - - [00-15] E 12 YML115C VAN1 LDB13|VRG8|VRG7 Component of the mannan polymerase I; complex contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant protein glycosylation, cell wall organization or biogenesis transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, Golgi apparatus, cytoplasm BY4742 [00-15] F 1 YMR037C MSN2 stress-responsive transcriptional activator MSN2 Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes; relative distribution to nucleus increases upon DNA replication stress response to heat, response to chemical, chromatin organization, response to oxidative stress, response to starvation, transcription from RNA polymerase II promoter, response to osmotic stress nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [00-15] F 2 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-15] F 3 YMR104C YPK2 putative protein kinase YPK2|YKR2 Protein kinase similar to S/T protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; involved in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6); human homolog SGK2 can complement a ypk1 ypk2 double mutant peptidyl-amino acid modification, protein phosphorylation kinase activity, transferase activity nucleus, cytoplasm BY4742 [00-15] F 4 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-15] F 5 - - - - - - - - [00-15] F 6 YMR119W ASI1 putative ubiquitin-protein ligase ASI1 Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; the Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids response to chemical, proteolysis involved in cellular protein catabolic process transferase activity nucleus, membrane, endomembrane system BY4742 [00-15] F 7 YNR052C POP2 CCR4-NOT core DEDD family RNase subunit POP2|CAF1 RNase of the DEDD superfamily; subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation RNA catabolic process, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation nuclease activity, hydrolase activity site of polarized growth, cytoplasm BY4742 [00-15] F 8 YNR055C HOL1 - Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake ion transport transmembrane transporter activity membrane, vacuole, mitochondrion, plasma membrane, cytoplasm BY4742 [00-15] F 9 YNR069C BSC5 BUL3 Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro biological_process molecular_function cellular_component BY4742 [00-15] F 10 YOL125W TRM13 tRNA:m4X modification enzyme 2'-O-methyltransferase; responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases tRNA processing, RNA modification transferase activity, methyltransferase activity nucleus, cytoplasm BY4742 [00-15] F 11 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-15] F 12 - - - - - - - - [00-15] G 1 YOL147C PEX11 PMP27|PMP24 Peroxisomal protein required for medium-chain fatty acid oxidation; also required for peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p organelle fission, peroxisome organization, monocarboxylic acid metabolic process, lipid metabolic process molecular_function peroxisome, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-15] G 2 - peroxisome, endomembrane system, membrane, endoplasmic reticulum, cytoplasm peroxisome, endomembrane system, membrane, endoplasmic reticulum, cytoplasm peroxisome, endomembrane system, membrane, endoplasmic reticulum, cytoplasm - - - - [00-15] G 3 - - - - - - - - [00-15] G 4 - - - - - - - - [00-15] G 5 - - - - - - - - [00-15] G 6 - - - - - - - - [00-15] G 7 YPL268W PLC1 phosphatidylinositol phospholipase C Phospholipase C; hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate the signaling molecules inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in regulating many cellular processes; Plc1p and inositol polyphosphates are required for acetyl-CoA homeostasis which regulates global histone acetylation lipid metabolic process, signaling hydrolase activity nucleus, chromosome BY4742 [00-15] G 8 YPR007C REC8 SPO69 Meiosis-specific component of the sister chromatid cohesion complex; alpha-kleisin family member that maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; independent of its role in sister chromatid cohesion, Rec8p promotes allelic collisions and prevents nonspecific chromosome interactions; homolog of S. pombe Rec8p meiotic cell cycle, organelle fission, DNA recombination, chromosome segregation chromatin binding nucleus, chromosome BY4742 [00-15] G 9 YPR008W HAA1 - Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity nucleus, cytoplasm BY4742 [00-15] G 10 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-15] G 11 - - - - - - - - [00-15] G 12 YPR013C CMR3 - Putative zinc finger protein; YPR013C is not an essential gene biological_process DNA binding cellular_component BY4742 [00-15] H 1 - cellular_component cellular_component cellular_component - - - - [00-15] H 2 YPR022C SDD4 - Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS; overproduction of a truncation allele suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P biological_process DNA binding nucleus, cytoplasm BY4742 [00-15] H 3 YPR023C EAF3 - Component of the Rpd3S histone deacetylase complex; Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition peptidyl-amino acid modification, DNA repair, protein acylation, chromatin organization, DNA replication, cellular response to DNA damage stimulus, histone modification, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation molecular_function nucleus, chromosome BY4742 [00-15] H 4 YPR024W YME1 i-AAA protease YME1|YTA11|OSD1 Catalytic subunit of i-AAA protease complex; complex is located in mitochondrial inner membrane; responsible for degradation of unfolded or misfolded mitochondrial gene products; serves as nonconventional translocation motor to pull PNPase into intermembrane space; also has role in intermembrane space protein folding; mutation causes elevated rate of mitochondrial turnover; human homolog YME1L1 can complement yeast null mutant protein targeting, mitochondrion organization, transmembrane transport, proteolysis involved in cellular protein catabolic process, protein maturation, protein folding peptidase activity, hydrolase activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-15] H 5 YPR026W ATH1 alpha,alpha-trehalase ATH1 Acid trehalase required for utilization of extracellular trehalose; involved in intracellular trehalose degradation during growth recovery after saline stress carbohydrate metabolic process, response to chemical, oligosaccharide metabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds cell wall, vacuole, cytoplasm BY4742 [00-15] H 6 YPR031W NTO1 - Subunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification histone binding nucleus, cytoplasm BY4742 [00-15] H 7 YPR037C ERV2 flavin-linked sulfhydryl oxidase Flavin-linked sulfhydryl oxidase localized to the ER lumen; involved in disulfide bond formation within the endoplasmic reticulum (ER) other isomerase activity, oxidoreductase activity endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-15] H 8 YPR043W RPL43A eL43|ribosomal 60S subunit protein L43A|L43e|L43A Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-15] H 9 YPR050C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps verified ORF MAK3/YPR051W biological_process molecular_function cellular_component BY4742 [00-15] H 10 YPR064W - - Putative protein of unknown function; conserved among S. cerevisiae strains; YPR064W is not an essential gene biological_process molecular_function cellular_component BY4742 [00-15] H 11 YPR067W ISA2 - Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations cofactor metabolic process, vitamin metabolic process, monocarboxylic acid metabolic process ion binding mitochondrion, cytoplasm BY4742 [00-15] H 12 YPR078C - - Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible biological_process molecular_function cellular_component BY4742 [00-16] A 1 YAR002C-A ERP1 - Member of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Golgi vesicle transport molecular_function cytoplasmic vesicle, endomembrane system, mitochondrion, cytoplasm BY4742 A 2 - cytoplasmic vesicle, endomembrane system, mitochondrion, cytoplasm cytoplasmic vesicle, endomembrane system, mitochondrion, cytoplasm cytoplasmic vesicle, endomembrane system, mitochondrion, cytoplasm - - - - [00-16] A 3 YBR083W TEC1 ROC1 Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member invasive growth in response to glucose limitation, pseudohyphal growth, transcription from RNA polymerase II promoter, transposition nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding nucleus BY4742 [00-16] A 4 YBR084C-A RPL19A eL19|ribosomal 60S subunit protein L19A|L19e|rpl5L|YL14|L23A|L19A Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 A 5 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - A 6 - - - - - - - - [00-16] A 7 YBR090C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus, cytoplasm BY4742 [00-16] A 8 YBR100W nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - BY4742 [00-16] A 9 YBR112C CYC8 transcription regulator CYC8|[OCT]|[OCT1+]|SSN6|CRT8 General transcriptional co-repressor; acts together with Tup1p; also acts as part of a transcriptional co-activator complex that recruits the SWI/SNF and SAGA complexes to promoters; can form the prion [OCT+] chromatin organization, regulation of transport, cellular response to DNA damage stimulus, monocarboxylic acid metabolic process, lipid metabolic process, transcription from RNA polymerase II promoter enzyme binding, transcription factor activity, protein binding nucleus BY4742 A 10 - nucleus nucleus nucleus - - - - [00-16] A 11 YBR125C PTC4 type 2C protein phosphatase PTC4|GCT1 Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity protein dephosphorylation phosphatase activity, hydrolase activity cytoplasm BY4742 [00-16] A 12 YBR131W CCZ1 CVT16 Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex, which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; involved in localizing Ypt7p to the vacuolar membrane; required for macroautophagy, the CVT pathway and mitophagy vacuole organization, protein targeting, organelle fusion guanyl-nucleotide exchange factor activity, lipid binding, ion binding cytoplasmic vesicle, endomembrane system, cytoplasm BY4742 [00-16] B 1 YBR150C TBS1 - Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication biological_process DNA binding nucleus, mitochondrion, cytoplasm BY4742 [00-16] B 2 YBR168W PEX32 - Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p peroxisome organization molecular_function peroxisome, membrane, cytoplasm BY4742 [00-16] B 3 YBR169C SSE2 adenyl-nucleotide exchange factor SSE2 Member of Hsp110 subclass of the heat shock protein 70 (HSP70) family; serves as nucleotide exchange factor to load ATP onto the SSA class of cytosolic Hsp70s; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication protein folding enzyme regulator activity cytoplasm BY4742 [00-16] B 4 YBR270C BIT2 - Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication signaling molecular_function other BY4742 [00-16] B 5 YBR272C HSM3 - Evolutionarily conserved 19S regulatory particle assembly-chaperone; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation; ortholog of human 19S subunit S5b DNA repair, cellular response to DNA damage stimulus, protein complex biogenesis molecular_function nucleus, cytoplasm BY4742 [00-16] B 6 YBR275C RIF1 DNA-binding protein RIF1 Protein that binds to the Rap1p C-terminus; acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; involved in control of DNA replication; contributes to resection of DNA double strand breaks (DSBs); deletion results in telomere elongation DNA repair, telomere organization, chromatin organization, DNA replication, cellular response to DNA damage stimulus DNA binding nucleus, chromosome BY4742 [00-16] B 7 YBR276C PPS1 tyrosine/serine/threonine protein phosphatase PPS1 Protein phosphatase; has specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle regulation of protein modification process, protein phosphorylation, DNA replication, protein dephosphorylation, signaling phosphatase activity, hydrolase activity cellular_component BY4742 [00-16] B 8 YBR280C SAF1 SCF ubiquitin ligase complex subunit SAF1 F-Box protein involved in proteasome-dependent degradation of Aah1p; involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 proteolysis involved in cellular protein catabolic process transferase activity other BY4742 [00-16] B 9 YBR287W - ZSP1 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-16] B 10 YBR288C APM3 YKS6 Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Golgi vesicle transport, protein targeting molecular_function membrane, cytoplasm BY4742 [00-16] B 11 YBR289W SNF5 TYE4|SWI10|HAF4 Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions carbohydrate metabolic process, DNA repair, response to chemical, invasive growth in response to glucose limitation, chromatin organization, cellular response to DNA damage stimulus, DNA recombination, transcription from RNA polymerase II promoter, oligosaccharide metabolic process hydrolase activity, ATPase activity, transcription factor binding nucleus, cytoplasm BY4742 [00-16] B 12 YBR294W SUL1 sulfate permease|SFP2 High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates transmembrane transport, ion transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-16] C 1 YBR301W PAU24 seripauperin PAU24|DAN3 Cell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth biological_process molecular_function cell wall BY4742 [00-16] C 2 YCL026C-A FRM2 type II nitroreductase|YCLX08C Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin response to chemical, lipid metabolic process, response to oxidative stress, monocarboxylic acid metabolic process oxidoreductase activity nucleus, cytoplasm BY4742 [00-16] C 3 YCR028C-A RIM1 - ssDNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication; stimulates utilization by Mip1p DNA polymerase of RNA primers synthesized by Rpo41p DNA replication, mitochondrion organization, regulation of organelle organization, regulation of DNA metabolic process DNA binding mitochondrion, cytoplasm BY4742 [00-16] C 4 YCR030C SYP1 YCR029C-A Negative regulator of WASP-Arp23 complex; involved in endocytic site formation; directly inhibits Las17p stimulation of Arp23 complex-mediated actin assembly in vitro; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization cytoskeleton organization enzyme regulator activity cellular bud, membrane, cell cortex, site of polarized growth, cytoskeleton, cytoplasm BY4742 [00-16] C 5 YCR032W BPH1 - Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting cell wall organization or biogenesis molecular_function membrane, vacuole, mitochondrion, cytoplasm BY4742 [00-16] C 6 YCR033W SNT1 - Subunit of the Set3C deacetylase complex; interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance; relocalizes to the cytosol in response to hypoxia protein phosphorylation, chromatin organization, histone modification, regulation of protein modification process, signaling molecular_function nucleus, chromosome, cytoplasm BY4742 [00-16] C 7 YCR046C IMG1 mitochondrial 54S ribosomal protein IMG1 Mitochondrial ribosomal protein of the large subunit; required for respiration and for maintenance of the mitochondrial genome mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-16] C 8 YCR047C BUD23 18S rRNA (guanine1575-N7)-methyltransferase Methyltransferase that methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern; functional homolog of human WBSCR22 mitotic cell cycle, rRNA processing, cytokinesis, ribosomal subunit export from nucleus, ribosomal small subunit biogenesis, nuclear transport, RNA modification transferase activity, methyltransferase activity nucleus, nucleolus, cytoplasm BY4742 [00-16] C 9 YCR048W ARE1 sterol acyltransferase|SAT2 Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication lipid metabolic process transferase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-16] C 10 YCR053W THR4 threonine synthase THR4 Threonine synthase; conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway cellular amino acid metabolic process lyase activity nucleus, cytoplasm BY4742 [00-16] C 11 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-16] C 12 - - - - - - - - [00-16] D 1 YCR060W TAH1 - Component of conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly of large protein complexes such as box C/D snoRNPs and RNA polymerase II; contains a single TPR domain with at least two TPR motifs; plays a role in determining prion variants protein folding other nucleus, cytoplasm BY4742 [00-16] D 2 YCR062W nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - BY4742 [00-16] D 3 - - - - - - - - [00-16] D 4 YCR067C SED4 - Integral ER membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; SED4 has a paralog, SEC12, that arose from the whole genome duplication regulation of organelle organization, regulation of transport, vesicle organization, protein complex biogenesis, Golgi vesicle transport enzyme regulator activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-16] D 5 YCR069W CPR4 peptidylprolyl isomerase family protein CPR4|SCC3|YCR070W|CYP4 Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; CPR4 has a paralog, CPR8, that arose from the whole genome duplication biological_process isomerase activity membrane, vacuole, cytoplasm BY4742 [00-16] D 6 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [00-16] D 7 YCR073C SSK22 mitogen-activated protein kinase kinase kinase SSK22 MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; functionally redundant with Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p; SSK22 has a paralog, SSK2, that arose from the whole genome duplication protein phosphorylation, signaling, response to osmotic stress kinase activity, transferase activity, signal transducer activity cellular_component BY4742 [00-16] D 8 YCR075C ERS1 cystinosin-like protein ERS1 Protein involved in cystine transport; localizes to the vacuole, plasma membrane and endosome; similarity to human cystinosin, a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains; mutation is functionally complemented by human CTNS transmembrane transport, ion transport, amino acid transport transmembrane transporter activity cytoplasmic vesicle, endomembrane system, vacuole, membrane, cytoplasm, plasma membrane BY4742 [00-16] D 9 YCR083W TRX3 - Mitochondrial thioredoxin; highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p response to chemical, response to oxidative stress oxidoreductase activity mitochondrion, cytoplasm BY4742 [00-16] D 10 YCR084C TUP1 chromatin-silencing transcriptional regulator TUP1|UMR7|SFL2|ROX4|FLK1|CYC9|CRT4|AMM1|AER2|AAR1 General repressor of transcription; forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes response to chemical, invasive growth in response to glucose limitation, chromatin organization, regulation of transport, cellular response to DNA damage stimulus, monocarboxylic acid metabolic process, lipid metabolic process, transcription from RNA polymerase II promoter histone binding, lipid binding, enzyme binding, ion binding, transcription factor activity, protein binding nucleus BY4742 [00-16] D 11 YCR088W ABP1 - Actin-binding protein of the cortical actin cytoskeleton; important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; inhibits barbed-end actin filament elongation; phosphorylation within its Proline-Rich Regio, mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences; mammalian homolog of HIP-55 (hematopoietic progenitor kinase 1 [HPK1]-interacting protein of 55 kDa) cytoskeleton organization, regulation of organelle organization, protein complex biogenesis cytoskeletal protein binding cell cortex, site of polarized growth, cytoskeleton, cytoplasm BY4742 [00-16] D 12 YCR089W FIG2 - Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating; FIG2 has a paralog, AGA1, that arose from the whole genome duplication response to chemical, invasive growth in response to glucose limitation, cell morphogenesis, conjugation molecular_function cell wall BY4742 [00-16] E 1 YDL194W SNF3 glucose sensor Plasma membrane low glucose sensor, regulates glucose transport; high affinity sensor that contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication meiotic cell cycle, response to chemical, organelle fission, regulation of organelle organization, carbohydrate transport, signaling, regulation of cell cycle other membrane, plasma membrane BY4742 [00-16] E 2 YDR007W TRP1 phosphoribosylanthranilate isomerase TRP1 Phosphoribosylanthranilate isomerase; catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA); enhances vegetative growth at low and high temperatures when used as an auxotrophic marker in strains such as W303 cellular amino acid metabolic process isomerase activity cytoplasm BY4742 [00-16] E 3 - cytoplasm cytoplasm cytoplasm - - - - [00-16] E 4 YDR048C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF VMS1/YDR049W biological_process molecular_function cellular_component BY4742 [00-16] E 5 - cellular_component cellular_component cellular_component - - - - [00-16] E 6 YFR011C MIC19 AIM13|MCS19 Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic19p is peripheral to the inner membrane and may connect Mic60p with the Mic10p-Mic12p-Mic27p subcomplex; both yeast and human Mic19p become oxidized, and oxidation may regulate MICOS mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-16] E 7 YFR013W IOC3 - Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; promotes nucleosome shifts in the 5 prime direction; IOC3 has a paralog, ESC8, that arose from the whole genome duplication chromatin organization, chromosome segregation chromatin binding, DNA binding, ATPase activity, hydrolase activity nucleus BY4742 [00-16] E 8 - nucleus nucleus nucleus - - - - [00-16] E 9 YNL051W COG5 Golgi transport complex subunit COG5|COD4|API4 Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Golgi vesicle transport, protein targeting molecular_function endomembrane system, cytoplasm, Golgi apparatus BY4742 [00-16] E 10 YNL052W COX5A cytochrome c oxidase subunit Va Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-16] E 11 YNL056W OCA2 - Protein of unknown function; similar to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene biological_process molecular_function cytoplasm BY4742 [00-16] E 12 - cytoplasm cytoplasm cytoplasm - - - - [00-16] F 1 YNL065W AQR1 - Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress response to chemical, transmembrane transport, ion transport, amino acid transport transmembrane transporter activity vacuole, membrane, cytoplasm, plasma membrane BY4742 [00-16] F 2 YNL066W SUN4 putative glucosidase SUN4|SCW3 Cell wall protein related to glucanases; possibly involved in cell wall septation; member of the SUN family; SUN4 has a paralog, SIM1, that arose from the whole genome duplication mitochondrion organization hydrolase activity, hydrolase activity, acting on glycosyl bonds cell wall, mitochondrion, cytoplasm BY4742 [00-16] F 3 YNL067W RPL9B uS4|uL6|ribosomal 60S subunit protein L9B|L6|rp24|YL11|L9B|L8B Ribosomal 60S subunit protein L9B; homologous to mammalian ribosomal protein L9 and bacterial L6; RPL9B has a paralog, RPL9A, that arose from a single-locus duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-16] F 4 YNL068C FKH2 forkhead family transcription factor FKH2 Forkhead family transcription factor; rate-limiting activator of replication origins; evolutionarily conserved regulator of lifespan; binds multiple chromosomal elements with distinct specificities, cell cycle dynamics; positively regulates transcriptional elongation; facilitates clustering, activation of early-firing replication origins; negative role in chromatin silencing at HML and HMR; major role in expression of G2/M phase genes; relocalizes to cytosol under hypoxia mitotic cell cycle, chromatin organization, DNA replication, pseudohyphal growth, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, regulation of cell cycle, DNA-templated transcription, elongation chromatin binding, DNA binding, transcription factor activity, protein binding, transcription factor binding nucleus, cytoplasm BY4742 [00-16] F 5 YNL070W TOM7 YOK22|MOM7 Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex protein targeting, mitochondrion organization, transmembrane transport protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-16] F 6 YNL071W LAT1 dihydrolipoyllysine-residue acetyltransferase|PDA2|ODP2 Dihydrolipoamide acetyltransferase component (E2) of the PDC; the pyruvate dehydrogenase complex (PDC) catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA cofactor metabolic process, monocarboxylic acid metabolic process transferase activity mitochondrion, cytoplasm BY4742 [00-16] F 7 YNL072W RNH201 ribonuclease H2 catalytic subunit RNH201|Rnh2A|RNH35 Ribonuclease H2 catalytic subunit; removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; role in ribonucleotide excision repair; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome RNA catabolic process, DNA replication nuclease activity, hydrolase activity nucleus BY4742 [00-16] F 8 YNL073W MSK1 lysine--tRNA ligase MSK1 Mitochondrial lysine-tRNA synthetase; required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, nucleobase-containing compound transport, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm BY4742 [00-16] F 9 YNL074C MLF3 YMK1 Serine-rich protein of unknown function; predicted to be palmitoylated; overproduction suppresses growth inhibition caused by exposure to immunosuppressant leflunomide; MLF3 has a paralog, VHS2, that arose from the whole genome duplication response to chemical molecular_function cytoplasm BY4742 [00-16] F 10 YNL076W MKS1 LYS80 Pleiotropic negative transcriptional regulator; involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling pseudohyphal growth, transcription from RNA polymerase II promoter, signaling molecular_function cytoplasm BY4742 [00-16] F 11 YNL078W NIS1 JIP1 Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network mitotic cell cycle, organelle fission, regulation of organelle organization, regulation of cell cycle molecular_function cellular bud, nucleus, site of polarized growth BY4742 [00-16] F 12 YNL079C TPM1 tropomyosin TPM1 Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication vacuole organization, cell budding, mitochondrion organization, cytoskeleton organization, organelle inheritance, exocytosis, pseudohyphal growth cytoskeletal protein binding cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [00-16] G 1 YNL080C EOS1 - Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene response to chemical, response to oxidative stress, protein glycosylation molecular_function endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-16] G 2 YNL082W PMS1 ATP-binding mismatch repair protein ATP-binding protein required for mismatch repair; required for both mitosis and meiosis; functions as a heterodimer with Mlh1p; binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL meiotic cell cycle, DNA repair, organelle fission, cellular response to DNA damage stimulus hydrolase activity, DNA binding, ion binding, ATPase activity nucleus, cytoplasm BY4742 [00-16] G 3 YNL083W SAL1 Ca(2+)-binding ATP:ADP antiporter SAL1 ADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains ion transport, nucleobase-containing compound transport transmembrane transporter activity, ion binding mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-16] G 4 YNL085W MKT1 - Protein similar to nucleases that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress; localization to P-bodies under ethanol stress differs between strains cellular response to DNA damage stimulus nuclease activity, hydrolase activity nucleus, cytoplasm BY4742 [00-16] G 5 YNL087W TCB2 tricalbin ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to bud; TCB2 has a paralog, TCB1, that arose from the whole genome duplication lipid metabolic process lipid binding cellular bud, cell cortex, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-16] G 6 YNL090W RHO2 Rho family GTPase RHO2 Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; involved in the establishment of cell polarity and in microtubule assembly cytoskeleton organization, cell wall organization or biogenesis, signaling GTPase activity, hydrolase activity membrane BY4742 [00-16] G 7 YNL091W NST1 - Protein of unknown function; mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 response to osmotic stress molecular_function cytoplasm BY4742 [00-16] G 8 YNL093W YPT53 Rab family GTPase YPT53 Stress-induced Rab family GTPase; required for vacuolar protein sorting and endocytosis; involved in ionic stress tolerance; similar to Vps21p and Ypt52p; functional homolog of Vps21p; mammalian Rab5 homolog; YPT53 has a paralog, VPS21, that arose from the whole genome duplication protein targeting, Golgi vesicle transport, endosomal transport, endocytosis GTPase activity, hydrolase activity cytoplasmic vesicle, endomembrane system, cytoplasm BY4742 [00-16] G 9 YNL095C - - Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-16] G 10 YNL097C PHO23 - Component of the Rpd3L histone deacetylase complex; involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus shares significant sequence identity with the human candidate tumor suppressor p33-ING1 and its isoform ING3 transcription from RNA polymerase I promoter, response to heat, invasive growth in response to glucose limitation, chromatin organization, transcription from RNA polymerase II promoter histone binding nucleus, chromosome BY4742 [00-16] G 11 YNL099C OCA1 putative tyrosine protein phosphatase OCA1 Putative protein tyrosine phosphatase; required for cell cycle arrest in response to oxidative damage of DNA response to chemical, response to oxidative stress phosphatase activity, hydrolase activity cytoplasm BY4742 [00-16] G 12 YNL100W MIC27 AIM37|MCS27 Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; forms a subcomplex with Mic10p and Mic12p whose assembly and stability requires cardiolipin mitochondrion organization molecular_function membrane, mitochondrial envelope, vacuole, mitochondrion, cytoplasm BY4742 [00-16] H 1 YNL104C LEU4 2-isopropylmalate synthase LEU4 Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication cellular amino acid metabolic process transferase activity mitochondrion, cytoplasm BY4742 [00-16] H 2 YNL105W RRT16 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription biological_process molecular_function cellular_component BY4742 [00-16] H 3 YNL106C INP52 phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP52|SJL2 Polyphosphatidylinositol phosphatase; dephosphorylates a number of phosphatidylinositol phosphates (PtdInsPs, PIPs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain; INP52 has a paralog, INP53, that arose from the whole genome duplication lipid metabolic process phosphatase activity, hydrolase activity cell cortex, site of polarized growth, cytoskeleton, cytoplasm BY4742 [00-16] H 4 YNL107W YAF9 - Subunit of NuA4 histone H4 acetyltransferase and SWR1 complexes; may function to antagonize silencing near telomeres; interacts directly with Swc4p; has homology to human leukemogenic protein AF9; contains a YEATS domain DNA repair, chromatin organization, cellular response to DNA damage stimulus molecular_function nucleus, chromosome, cytoplasm BY4742 [00-16] H 5 YNL115C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [00-16] H 6 YNL119W NCS2 TUC2 Protein required for uridine thiolation of Lys(UUU) and Glu(UUC) tRNAs; required for the thiolation of uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae tRNA processing, invasive growth in response to glucose limitation, protein modification by small protein conjugation or removal, pseudohyphal growth, RNA modification molecular_function cytoplasm BY4742 [00-16] H 7 YNL120C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene biological_process molecular_function cellular_component BY4742 [00-16] H 8 YNL121C TOM70 protein channel TOM70|OMP1|MOM72|MAS70 Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication protein targeting, mitochondrion organization, transmembrane transport protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-16] H 9 YNL125C ESBP6 MCH3 Protein with similarity to monocarboxylate permeases; appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane other other membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [00-16] H 10 - membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm - - - - [00-16] H 11 YNL130C CPT1 diacylglycerol cholinephosphotransferase Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication lipid metabolic process transferase activity endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4742 [00-16] H 12 - endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, Golgi apparatus, cytoplasm endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, Golgi apparatus, cytoplasm endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, Golgi apparatus, cytoplasm - - - - [00-17] A 1 YAL024C LTE1 mitotic regulator LTE1|MSI2 Protein similar to GDP/GTP exchange factors; without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures mitotic cell cycle, organelle fission, regulation of organelle organization, regulation of cell cycle, chromosome segregation guanyl-nucleotide exchange factor activity cellular bud BY4742 [00-17] A 2 YBR299W MAL32 alpha-glucosidase MAL32|MALS|MAL3S Maltase (alpha-D-glucosidase); inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose carbohydrate metabolic process, oligosaccharide metabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds membrane, plasma membrane BY4742 [00-17] A 3 - membrane, plasma membrane membrane, plasma membrane membrane, plasma membrane - - - - [00-17] A 4 - - - - - - - - [00-17] A 5 YCR107W AAD3 putative aryl-alcohol dehydrogenase Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; AAD3 has a paralog, AAD15, that arose from a segmental duplication; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family other oxidoreductase activity cellular_component BY4742 [00-17] A 6 - cellular_component cellular_component cellular_component - - - - [00-17] A 7 - - - - - - - - [00-17] A 8 YDR242W AMD2 putative amidase Putative amidase biological_process hydrolase activity cellular_component BY4742 [00-17] A 9 YDR326C YSP2 LAM2|LTC4 Sterol-binding protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; contains two StART-like domains that bind sterols and a GRAM domain; co-localizes to puncta in the cortical ER with Sip3p; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes lipid transport lipid binding cell cortex, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [00-17] A 10 YDR417C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W biological_process molecular_function membrane BY4742 [00-17] A 11 YDR444W - putative hydrolase Putative hydrolase acting on ester bonds biological_process molecular_function cytoplasm BY4742 [00-17] A 12 YDR461W MFA1 mating pheromone a Mating pheromone a-factor; made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 response to chemical, conjugation, signaling other endomembrane system, extracellular region, endoplasmic reticulum, cytoplasm BY4742 [00-17] B 1 YDR493W MZM1 AIM8|FMP36 Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; member of the LYR protein family; human LYRM7 is a functional ortholog mitochondrion organization, protein complex biogenesis other mitochondrion, cytoplasm BY4742 [00-17] B 2 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-17] B 3 YDR500C RPL37B eL37|ribosomal 60S subunit protein L37B|L37e|YL35|L43|L37B Ribosomal 60S subunit protein L37B; required for processing of 27SB pre-rRNA and formation of stable 66S assembly intermediates; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal large subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-17] B 4 YDR502C SAM2 methionine adenosyltransferase SAM2|ETH2 S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; comparative analysis suggests that a mitochondrially targeted form may result from translation starting at a non-canonical codon upstream of the annotated start codon cofactor metabolic process, cellular amino acid metabolic process transferase activity cytoplasm BY4742 [00-17] B 5 YDR506C GMC1 putative oxidoreductase Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively meiotic cell cycle, organelle fission molecular_function cellular_component BY4742 [00-17] B 6 YDR512C EMI1 - Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs; deletion affects mitochondrial morphology meiotic cell cycle, sporulation, mitochondrion organization, transcription from RNA polymerase II promoter molecular_function cellular_component BY4742 [00-17] B 7 YDR515W SLF1 SRO99 RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cellular ion homeostasis, regulation of translation RNA binding, mRNA binding cytoplasm BY4742 [00-17] B 8 - cytoplasm cytoplasm cytoplasm - - - - [00-17] B 9 YER089C PTC2 type 2C protein phosphatase PTC2 Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication response to chemical, protein phosphorylation, protein dephosphorylation, cellular response to DNA damage stimulus, regulation of protein modification process, signaling, response to osmotic stress, regulation of cell cycle phosphatase activity, hydrolase activity nucleus, cytoplasm BY4742 [00-17] B 10 YFL001W DEG1 pseudouridine synthase DEG1|HRM3|PUS3 tRNA:pseudouridine synthase; introduces pseudouridines at position 38 or 39 in tRNA; also responsible for pseudouracil modification of some mRNAs; important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability tRNA processing, RNA modification isomerase activity nucleus, cytoplasm BY4742 [00-17] B 11 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-17] B 12 YFL003C MSH4 MutS family protein MSH4 Protein involved in meiotic recombination; required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein meiotic cell cycle, organelle fission, DNA recombination DNA binding nucleus, chromosome BY4742 [00-17] C 1 YFL004W VTC2 vacuolar transporter chaperone|PHM1 Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC2 has a paralog, VTC3, that arose from the whole genome duplication vacuole organization, organelle fusion, membrane fusion molecular_function endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-17] C 2 - endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm - - - - [00-17] C 3 YFL007W BLM10 YFL006W Proteasome activator; binds the core proteasome (CP) and stimulates proteasome-mediated protein degradation by inducing gate opening; required for sequestering CP into proteasome storage granule (PSG) during quiescent phase and for nuclear import of CP in proliferating cells; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 protein targeting, proteolysis involved in cellular protein catabolic process, protein complex biogenesis, nuclear transport enzyme regulator activity nucleus, membrane, cytoplasm BY4742 [00-17] C 4 YFL010C WWM1 - WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 biological_process molecular_function nucleus, mitochondrion, cytoplasm BY4742 [00-17] C 5 YFL010W-A AUA1 YFL011W-A Protein required for the negative regulation by ammonia of Gap1p; Gap1p is a general amino acid permease ion transport, amino acid transport molecular_function cellular_component BY4742 [00-17] C 6 YFL012W - - Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin biological_process molecular_function cellular_component BY4742 [00-17] C 7 YFL013C IES1 - Subunit of the INO80 chromatin remodeling complex; relocalizes to the cytosol in response to hypoxia chromatin organization molecular_function nucleus, chromosome, cytoplasm BY4742 [00-17] C 8 YFL016C MDJ1 - Co-chaperone that stimulates HSP70 protein Ssc1p ATPase activity; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones response to heat, mitochondrion organization, proteolysis involved in cellular protein catabolic process, protein folding enzyme regulator activity, unfolded protein binding mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 - [00-17] C 9 YFL033C RIM15 protein kinase RIM15|TAK1 Protein kinase involved in cell proliferation in response to nutrients; glucose-repressible; involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; phosphorylates Igo1p and Igo2p; substrate of Pho80p-Pho85p kinase response to heat, meiotic cell cycle, response to chemical, protein phosphorylation, organelle fission, regulation of organelle organization, response to oxidative stress, response to starvation, regulation of cell cycle kinase activity, transferase activity nucleus, cytoplasm BY4742 [00-17] C 10 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-17] C 11 YGR122C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; similar to YLR334C and YOL106W biological_process molecular_function cellular_component BY4742 [00-17] C 12 YGR162W TIF4631 translation initiation factor eIF4G|eiF4G1 Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication translational initiation, organelle assembly, ribosomal large subunit biogenesis RNA binding, mRNA binding, translation factor activity, RNA binding mitochondrion, cytoplasm BY4742 [00-17] D 1 YGR252W GCN5 histone acetyltransferase GCN5|AAS104|KAT2|SWI9|ADA4 Catalytic subunit of ADA and SAGA histone acetyltransferase complexes; modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; relocalizes to the cytosol in response to hypoxia; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation transferase activity, histone binding, transcription factor activity, protein binding nucleus, chromosome, cytoplasm BY4742 [00-17] D 2 YGR254W ENO1 phosphopyruvate hydratase ENO1|HSP48 Enolase I, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression repressed in response to glucose; protein abundance increases in response to DNA replication stress; N-terminally propionylated in vivo; ENO1 has a paralog, ENO2, that arose from the whole genome duplication vacuole organization, carbohydrate metabolic process, cofactor metabolic process, regulation of organelle organization, organelle fusion, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, membrane fusion, nucleobase-containing small molecule metabolic process lyase activity vacuole, mitochondrion, membrane, cytoplasm, plasma membrane BY4742 [00-17] D 3 YGR255C COQ6 putative N,N-dimethylaniline monooxygenase COQ6 Flavin-dependent monooxygenase involved in ubiquinone biosynthesis; responsible for hydroxylation at position C5 and deamination at C4 during ubiquinone (Coenzyme Q) biosynthesis; localizes to matrix face of mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human homolog COQ6 can complement yeast null mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) cofactor metabolic process oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-17] D 4 YGR257C MTM1 - Mitochondrial protein of the mitochondrial carrier family; high affinity pyridoxal 5'-phosphate (PLP) transporter, important for delivery of PLP cofactor to mitochondrial enzymes; involved in mitochondrial iron homeostasis and in activating mitochondrial Sod2p by facilitating insertion of an essential manganese cofactor ion transport ion binding membrane, mitochondrion, cytoplasm BY4742 [00-17] D 5 YGR258C RAD2 ssDNA endodeoxyribonuclease RAD2 Single-stranded DNA endonuclease; cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein DNA repair, cellular response to DNA damage stimulus, transcription from RNA polymerase II promoter nuclease activity, hydrolase activity nucleus BY4742 [00-17] D 6 YGR271W SLH1 putative RNA helicase Putative RNA helicase related to Ski2p; involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses cytoplasmic translation, regulation of translation RNA binding, mRNA binding, helicase activity, hydrolase activity ribosome, cytoplasm BY4742 [00-17] D 7 YGR272C ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - BY4742 [00-17] D 8 YGR273C - IBI1 Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; not an essential gene; YGR273C has a paralog, YMR295C, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4742 [00-17] D 9 YGR276C RNH70 RNA82|REX1 3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts tRNA processing, rRNA processing nuclease activity, hydrolase activity nucleus BY4742 [00-17] D 10 YGR289C MAL11 alpha-glucoside permease|MALT|AGT1 High-affinity maltose transporter (alpha-glucoside transporter); inducible; encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization carbohydrate metabolic process, carbohydrate transport, oligosaccharide metabolic process transmembrane transporter activity membrane BY4742 [00-17] D 11 YGR291C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-17] D 12 YGR292W MAL12 alpha-glucosidase MAL12|MALS Maltase (alpha-D-glucosidase); inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose carbohydrate metabolic process, oligosaccharide metabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds membrane, plasma membrane BY4742 [00-17] E 1 YGR295C COS6 - Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins protein targeting, proteolysis involved in cellular protein catabolic process, endosomal transport molecular_function cytoplasmic vesicle, endomembrane system, vacuole, membrane, cytoplasm BY4742 [00-17] E 2 YHR132W-A IGO2 phosphatase regulator Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; IGO2 has a paralog, IGO1, that arose from the whole genome duplication other enzyme regulator activity nucleus, cytoplasm BY4742 [00-17] E 3 YIL030C SSM4 E3 ubiquitin-protein ligase SSM4|KIS3|DOA10 Membrane-embedded ubiquitin-protein ligase; ER and inner nuclear membrane localized RING-CH domain E3 ligase involved in ER-associated protein degradation (ERAD); targets misfolded cytosolic/nucleoplasmic domains of soluble and membrane embedded proteins (ERAD-C) and a transmembrane domain containing substrate (ERAD-M), Sbh2p; C-terminal element (CTE), conserved in human ortholog MARCH10/TEB4, determines substrate selectivity response to chemical, proteolysis involved in cellular protein catabolic process transferase activity nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [00-17] E 4 - nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm - - - - [00-17] E 5 YIL058W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-17] E 6 YIL092W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus biological_process molecular_function nucleus, cytoplasm BY4742 [00-17] E 7 YIR023W DAL81 DURL|UGA35 Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism response to chemical, transcription from RNA polymerase II promoter, monocarboxylic acid metabolic process, cellular amino acid metabolic process transcription factor activity, protein binding nucleus BY4742 [00-17] E 8 YIR030C DCG1 - Protein of unknown function; expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain other molecular_function cellular_component BY4742 [00-17] E 9 YIR032C DAL3 ureidoglycolate hydrolase Ureidoglycolate lyase; converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression is sensitive to nitrogen catabolite repression; this enzyme is sometimes referred to "ureidoglycolate hydrolase" but should not be confused with the Arabidopsis thaliana ureidoglycolate hydrolase enzyme which converts ureidoglycolate to glyoxylate, ammonia and carbon dioxide other lyase activity membrane BY4742 [00-17] E 10 YIR043C membrane membrane membrane - - - BY4742 [00-17] E 11 YIR044C - - - - - - BY4742 [00-17] E 12 YJR003C MRX12 - Protein that associates with mitochondrial ribosome; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis biological_process molecular_function mitochondrion, cytoplasm BY4742 [00-17] F 1 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-17] F 2 YJR055W HIT1 - Protein involved in C/D snoRNP assembly; regulates abundance of Rsa1p; required for growth at high temperature; similar to human ZNHIT3 rRNA processing, ribosomal large subunit biogenesis molecular_function nucleus, cytoplasm BY4742 [00-17] F 3 YKL053C-A MDM35 - Mitochondrial intermembrane space protein; forms complex with Ups2p that transfers phosphatidylserine from outer membrane to inner membrane for phosphatidylethanolamine synthesis; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress mitochondrion organization, ion transport, protein complex biogenesis, lipid transport other nucleus, mitochondrion, mitochondrial envelope, cytoplasm BY4742 [00-17] F 4 YKR106W GEX2 glutathione exchanger Proton:glutathione antiporter; localized to the vacuolar and plasma membranes; expressed at a very low level; potential role in resistance to oxidative stress and modulation of the PKA pathway; GEX2 has a paralog, GEX1, that arose from a segmental duplication transmembrane transport, ion transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [00-17] F 5 - membrane, vacuole, plasma membrane, cytoplasm membrane, vacuole, plasma membrane, cytoplasm membrane, vacuole, plasma membrane, cytoplasm - - - - [00-17] F 6 YMR191W SPG5 - Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources protein complex biogenesis other cellular_component BY4742 [00-17] F 7 YMR322C SNO4 glutathione-independent methylglyoxalase family protein|HSP34 Possible chaperone and cysteine protease; required for transcriptional reprogramming during the diauxic shift and for survival in stationary phase; similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation other peptidase activity, hydrolase activity cellular_component BY4742 [00-17] F 8 YNL138W SRV2 adenylate cyclase-binding protein|CAP CAP (cyclase-associated protein); N-terminus binds adenylate cyclase and facilitates activation by RAS; N-terminus forms novel hexameric star-shaped shuriken structures that directly catalyze cofilin-mediated severing of actin filaments; C-terminus binds and recycles cofilin bound, ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis; N- and C-termini can function as physically separate proteins; mCAP1 is the mouse homolog cytoskeleton organization, regulation of organelle organization, signaling enzyme binding, cytoskeletal protein binding cell cortex, cytoskeleton, site of polarized growth, cytoplasm BY4742 [00-17] F 9 YNL140C - - Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C biological_process molecular_function cellular_component BY4742 [00-17] F 10 YNL142W MEP2 ammonium permease MEP2 Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation pseudohyphal growth, ion transport transmembrane transporter activity membrane, plasma membrane BY4742 [00-17] F 11 YNL315C ATP11 - Molecular chaperone; required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; N-terminally propionylated in vivo mitochondrion organization, protein complex biogenesis unfolded protein binding mitochondrion, cytoplasm BY4742 [00-17] F 12 YOL151W GRE2 methylglyoxal reductase (NADPH-dependent) GRE2 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; restores resistance to glycolaldehyde by coupling reduction of glycolaldehyde to ethylene glycol and oxidation of NADPH to NADP+; protein abundance increases in response to DNA replication stress; methylglyoxal reductase (NADPH-dependent) is also known as D-lactaldehyde dehydrogenase lipid metabolic process oxidoreductase activity nucleus, cytoplasm BY4742 [00-17] G 1 YOL152W FRE7 putative ferric-chelate reductase Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels ion transport oxidoreductase activity membrane, plasma membrane BY4742 [00-17] G 2 YOL155C HPF1 mannoprotein Haze-protective mannoprotein; reduces the particle size of aggregated proteins in white wines cell wall organization or biogenesis hydrolase activity, hydrolase activity, acting on glycosyl bonds cell wall, extracellular region BY4742 [00-17] G 3 YOR265W RBL2 - Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress protein complex biogenesis cytoskeletal protein binding cytoplasm BY4742 [00-17] G 4 YOR266W PNT1 - Mitochondrial integral inner membrane protein; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-17] G 5 YOR267C HRK1 putative serine/threonine protein kinase HRK1 Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress cellular ion homeostasis, protein phosphorylation kinase activity, transferase activity cytoplasm BY4742 [00-17] G 6 YOR268C - - Putative protein of unknown function; sporulation is abnormal in homozygous diploid; SWAT-GFP fusion protein localizes to the nucleus; YOR268C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-17] G 7 YOR269W PAC1 - Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly other cytoskeletal protein binding nucleus, cytoskeleton, cytoplasm BY4742 [00-17] G 8 YOR270C VPH1 H(+)-transporting V0 sector ATPase subunit a Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress; human homolog ATP6V0A4 implicated in renal tubular acidosis, can complement yeast null mutant ion transport, protein complex biogenesis, cellular ion homeostasis hydrolase activity, transmembrane transporter activity, ATPase activity vacuole, membrane, cytoplasm BY4742 [00-17] G 9 YOR271C FSF1 - Putative protein; predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis biological_process molecular_function membrane, mitochondrion, cytoplasm BY4742 [00-17] G 10 - membrane, mitochondrion, cytoplasm membrane, mitochondrion, cytoplasm membrane, mitochondrion, cytoplasm - - - - [00-17] G 11 YOR273C TPO4 - Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane ion transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [00-17] G 12 YOR274W MOD5 tRNA dimethylallyltransferase|[MOD+] Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; also has a role in tRNA gene-mediated silencing; gene encodes two isozymic forms; converts to a prion form, prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis; homolog of human TRIT1, a mutation in which is associated with severe combined respiratory chain defects chromatin organization, tRNA processing, transcription from RNA polymerase II promoter, RNA modification RNA binding, transferase activity nucleus, mitochondrion, nucleolus, cytoplasm BY4742 [00-17] H 1 YOR275C RIM20 - Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation invasive growth in response to glucose limitation, sporulation, protein maturation molecular_function nucleus, cytoplasm BY4742 [00-17] H 2 YOR276W CAF20 p20|CAP20|CAF2 Phosphoprotein of the mRNA cap-binding complex; involved in translational control; repressor of cap-dependent translation initiation; competes with eIF4G for binding to eIF4E regulation of translation, regulation of organelle organization, organelle assembly molecular_function cytoplasm BY4742 [00-17] H 3 YOR298C-A MBF1 SUF13 Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress transcription from RNA polymerase II promoter transcription factor activity, protein binding nucleus, mitochondrion, cytoplasm BY4742 [00-17] H 4 YOR302W - - CPA1 uORF; Arginine attenuator peptide, regulates translation of the CPA1 mRNA regulation of translation other cytoplasm BY4742 [00-17] H 5 YOR303W CPA1 carbamoyl-phosphate synthase (glutamine-hydrolyzing) CPA1 Small subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader cellular amino acid metabolic process ligase activity cytoplasm BY4742 [00-17] H 6 YPL004C LSP1 lipid-binding protein LSP1 Eisosome core component; eisosomes are large immobile patch structures at the cell cortex associated with endocytosis; phosphorylated on Thr233 upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family response to heat, protein phosphorylation, regulation of protein modification process, endocytosis lipid binding mitochondrion, membrane, mitochondrial envelope, cytoplasm, plasma membrane BY4742 [00-17] H 7 YPL017C IRC15 - Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci; IRC15 has a paralog, LPD1, that arose from the whole genome duplication mitotic cell cycle, meiotic cell cycle, organelle fission, cytoskeleton organization, protein complex biogenesis, chromosome segregation, DNA recombination, regulation of cell cycle transferase activity, methyltransferase activity, cytoskeletal protein binding cytoskeleton, cytoplasm BY4742 [00-17] H 8 YPL027W SMA1 - Protein of unknown function involved in prospore membrane assembly; involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p meiotic cell cycle, sporulation molecular_function membrane BY4742 [00-17] H 9 YPL034W - - Putative protein of unknown function; YPL034W is not essential gene biological_process molecular_function cellular_component BY4742 [00-17] H 10 YPL036W PMA2 H(+)-exporting P2-type ATPase PMA2 Plasma membrane H+-ATPase; isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential ion transport ATPase activity, hydrolase activity, transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm BY4742 [00-17] H 11 YPL078C ATP4 F1F0 ATP synthase subunit 4|LPF7 Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase; ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex, which in turn determines the shape of inner membrane cristae; phosphorylated transmembrane transport, ion transport, protein complex biogenesis, nucleobase-containing small molecule metabolic process hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-17] H 12 YPL137C GIP3 protein phosphatase regulator GIP3 Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication protein dephosphorylation, chromosome segregation, regulation of protein modification process enzyme regulator activity, enzyme binding endomembrane system, ribosome, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [00-18] A 1 YBR020W GAL1 galactokinase Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; human homolog GALK2 complements yeast null mutant; GAL1 has a paralog, GAL3, that arose from the whole genome duplication carbohydrate metabolic process, response to chemical kinase activity, transferase activity cytoplasm BY4742 [00-18] A 2 YBR075W cytoplasm cytoplasm cytoplasm - - - BY4742 [00-18] A 3 YDR417C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W biological_process molecular_function membrane BY4742 [00-18] A 4 - membrane membrane membrane - - - - [00-18] A 5 - - - - - - - - [00-18] A 6 - - - - - - - - [00-18] A 7 - - - - - - - - [00-18] A 8 - - - - - - - - [00-18] A 9 - - - - - - - - [00-18] A 10 - - - - - - - - [00-18] A 11 - - - - - - - - [00-18] A 12 - - - - - - - - [00-18] B 1 - - - - - - - - [00-18] B 2 - - - - - - - - [00-18] B 3 - - - - - - - - [00-18] B 4 - - - - - - - - [00-18] B 5 - - - - - - - - [00-18] B 6 YFL033C RIM15 protein kinase RIM15|TAK1 Protein kinase involved in cell proliferation in response to nutrients; glucose-repressible; involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; phosphorylates Igo1p and Igo2p; substrate of Pho80p-Pho85p kinase response to heat, meiotic cell cycle, response to chemical, protein phosphorylation, organelle fission, regulation of organelle organization, response to oxidative stress, response to starvation, regulation of cell cycle kinase activity, transferase activity nucleus, cytoplasm BY4742 [00-18] B 7 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [00-18] B 8 YFL063W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [00-18] B 9 - cellular_component cellular_component cellular_component - - - - [00-18] B 10 - - - - - - - - [00-18] B 11 YJL103C GSM1 - Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis nucleobase-containing small molecule metabolic process, transcription from RNA polymerase II promoter, generation of precursor metabolites and energy nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [00-18] B 12 YML073C RPL6A eL6|ribosomal 60S subunit protein L6A|L6e|rp18|YL16|L6A|L17A|YL16A Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly RNA binding, structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [00-18] C 1 YNL011C - - Putative protein of unknown function; YNL011C is not an essential gene biological_process molecular_function cellular_component BY4742 [00-18] C 2 YNL014W HEF3 translation elongation factor EF-3|ZRG7 Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication translational elongation RNA binding, translation factor activity, RNA binding, ATPase activity, hydrolase activity ribosome, cytoplasm BY4742 [00-18] C 3 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [00-18] C 4 YNL047C SLM2 phosphatidylinositol 4,5-bisphosphate-binding protein|LIT1 Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; TORC2 complex substrate and effector; SLM2 has a paralog, SLM1, that arose from the whole genome duplication endosomal transport, cytoskeleton organization, signaling ion binding, lipid binding membrane, plasma membrane BY4742 [00-18] C 5 YNL053W MSG5 tyrosine/serine/threonine protein phosphatase MSG5 Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p; MSG5 has a paralog, SDP1, that arose from the whole genome duplication response to chemical, protein phosphorylation, protein dephosphorylation, regulation of transport, regulation of protein modification process, conjugation, cell wall organization or biogenesis, nuclear transport, signaling phosphatase activity, hydrolase activity nucleus, cytoplasm BY4742 [00-18] C 6 YNL055C POR1 porin POR1|VDAC|YVDAC1|OMP2 Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; couples the glutathione pools of the intermembrane space (IMS) and the cytosol; interacts with Om45 and Om14 in the outer membrane; phosphorylated; protein abundance increases in response to DNA replication stress mitochondrion organization, ion transport, nucleobase-containing compound transport transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-18] C 7 YNL059C ARP5 - Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes; promotes nucleosome shifts in the 3 prime direction chromatin organization helicase activity, ATPase activity, hydrolase activity nucleus, chromosome BY4742 [00-18] C 8 YNL069C RPL16B uL13|ribosomal 60S subunit protein L16B|L13|rp23|YL15|L21B|L16B|RP23 Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-18] C 9 YNL086W SNN1 BLS1 Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; green fluorescent protein (GFP)-fusion protein localizes to endosomes vesicle organization molecular_function cytoplasmic vesicle, endomembrane system, cytoplasm BY4742 [00-18] C 10 YNL089C - - Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins biological_process molecular_function cellular_component BY4742 [00-18] C 11 YNL096C RPS7B eS7|ribosomal 40S subunit protein S7B|S7e|rp30|S7B Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [00-18] C 12 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [00-18] D 1 YNL109W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C biological_process molecular_function membrane BY4742 [00-18] D 2 YNL111C CYB5 - Cytochrome b5; involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation lipid metabolic process other membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [00-18] D 3 YNL147W LSM7 Sm-like protein LSM7 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress RNA splicing, rRNA processing, ribosomal small subunit biogenesis, RNA catabolic process, mRNA processing RNA binding nucleus, nucleolus, cytoplasm BY4742 [00-18] D 4 YNL220W ADE12 adenylosuccinate synthase|BRA9 Adenylosuccinate synthase; catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence nucleobase-containing small molecule metabolic process DNA binding, ligase activity cytoplasm BY4742 [00-18] D 5 YNL268W LYP1 lysine permease Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids ion transport, amino acid transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm BY4742 [00-18] D 6 YNL284C MRPL10 mitochondrial 54S ribosomal protein YmL10/YmL18|YmL10|YmL18|MRPL18 Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-18] D 7 YNL315C ATP11 - Molecular chaperone; required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; N-terminally propionylated in vivo mitochondrion organization, protein complex biogenesis unfolded protein binding mitochondrion, cytoplasm BY4742 [00-18] D 8 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-18] D 9 YNR033W ABZ1 4-amino-4-deoxychorismate synthase Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress monocarboxylic acid metabolic process, cellular amino acid metabolic process transferase activity cytoplasm BY4742 [00-18] D 10 YOL148C SPT20 ADA5 Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay peptidyl-amino acid modification, response to chemical, protein acylation, RNA splicing, chromatin organization, histone modification, mRNA processing, signaling transcription factor activity, protein binding nucleus BY4742 [00-18] D 11 YOL151W GRE2 methylglyoxal reductase (NADPH-dependent) GRE2 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; restores resistance to glycolaldehyde by coupling reduction of glycolaldehyde to ethylene glycol and oxidation of NADPH to NADP+; protein abundance increases in response to DNA replication stress; methylglyoxal reductase (NADPH-dependent) is also known as D-lactaldehyde dehydrogenase lipid metabolic process oxidoreductase activity nucleus, cytoplasm BY4742 [00-18] D 12 YOL152W FRE7 putative ferric-chelate reductase Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels ion transport oxidoreductase activity membrane, plasma membrane BY4742 [00-18] E 1 - membrane, plasma membrane membrane, plasma membrane membrane, plasma membrane - - - - [00-18] E 2 YPL158C AIM44 GPS1 Protein that regulates Cdc42p and Rho1p; functions in the late steps of cytokinesis and cell separation; sustains Rho1p at the cell division site after actomyosin ring contraction; inhibits the activation of Cdc42-Cla4 at the cell division site to prevent budding inside the old bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress mitotic cell cycle, cytokinesis molecular_function cellular bud, site of polarized growth BY4742 [00-18] E 3 YPL183W-A RTC6 putative mitochondrial 54S ribosomal protein RTC6|TAE4|GON5 Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [00-18] E 4 YPL194W DDC1 - DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress mitotic cell cycle, meiotic cell cycle, DNA repair, organelle fission, regulation of organelle organization, cellular response to DNA damage stimulus, DNA recombination, regulation of cell cycle molecular_function nucleus, chromosome BY4742 [00-18] E 5 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [00-18] E 6 - - - - - - - - [00-18] E 7 YPR011C - - Mitochondrial transporter; major substrates are adenosine 5'-phosphosulfate (APS) and 3'-phospho-adenosine 5'-phosphosulfate (PAPS); member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies transmembrane transport, ion transport, nucleobase-containing compound transport transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-18] E 8 YPR021C AGC1 amino acid transporter AGC1 Mitochondrial amino acid transporter; acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis; human homolog SLC25A13 complements yeast null mutant ion transport, amino acid transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-18] E 9 YPR083W MDM36 - Mitochondrial protein; required for normal mitochondrial morphology and inheritance; component of the mitochondria-ER-cortex-ancor (MECA); interacts with Num1p to link the ER and mitochondria at the cell cortex; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission organelle fission, organelle inheritance, mitochondrion organization molecular_function mitochondrion, cytoplasm BY4742 [00-18] E 10 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [00-18] E 11 YPR091C NVJ2 - Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial-lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments biological_process lipid binding endomembrane system, ribosome, endoplasmic reticulum, cytoplasm BY4742 [00-18] E 12 - endomembrane system, ribosome, endoplasmic reticulum, cytoplasm endomembrane system, ribosome, endoplasmic reticulum, cytoplasm endomembrane system, ribosome, endoplasmic reticulum, cytoplasm - - - - [00-18] F 1 YPR118W MRI1 S-methyl-5-thioribose-1-phosphate isomerase MRI1 5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway cellular amino acid metabolic process isomerase activity nucleus, cytoplasm BY4742 [00-18] F 2 YPR133W-A TOM5 MOM8A Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore protein targeting, mitochondrion organization, protein complex biogenesis protein transporter activity, transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [00-18] F 3 YPR151C SUE1 - Protein required for degradation of unstable forms of cytochrome c; located in the mitochondria other molecular_function mitochondrial envelope, mitochondrion, cytoplasm BY4742 [00-18] F 4 YMR074C SDD2 - Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress response to chemical, response to oxidative stress molecular_function nucleus, cytoplasm BY4742 [00-18] F 5 YMR118C SHH3 protein SHH3 Putative mitochondrial inner membrane protein of unknown function; although similar to paralogous Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase; SHH3 has a paralog, SDH3, that arose from the whole genome duplication biological_process molecular_function membrane, mitochondrion, cytoplasm BY4742 [00-18] F 6 YOL153C membrane, mitochondrion, cytoplasm membrane, mitochondrion, cytoplasm membrane, mitochondrion, cytoplasm - - - BY4742 [00-18] F 7 - - - - - - - - [00-18] F 8 - - - - - - - - [00-18] F 9 - - - - - - - - [00-18] F 10 - - - - - - - - [00-18] F 11 - - - - - - - - [00-18] F 12 - - - - - - - - [00-18] G 1 - - - - - - - - [00-18] G 2 - - - - - - - - [00-18] G 3 - - - - - - - - [00-18] G 4 - - - - - - - - [00-18] G 5 - - - - - - - - [00-18] G 6 - - - - - - - - [00-18] G 7 - - - - - - - - [00-18] G 8 - - - - - - - - [00-18] G 9 - - - - - - - - [00-18] G 10 - - - - - - - - [00-18] G 11 - - - - - - - - [00-18] G 12 - - - - - - - - [00-18] H 1 - - - - - - - - [00-18] H 2 - - - - - - - - [00-18] H 3 - - - - - - - - [00-18] H 4 - - - - - - - - [00-18] H 5 - - - - - - - - [00-18] H 6 - - - - - - - - [00-18] H 7 - - - - - - - - [00-18] H 8 - - - - - - - - [00-18] H 9 - - - - - - - - [00-18] H 10 - - - - - - - - [00-18] H 11 - - - - - - - - [00-18] H 12 - - - - - - - - [1-1] A 1 - - - - - - - - [1-1] A 2 YAL068C PAU8 seripauperin PAU8 Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions meiotic cell cycle, sporulation, transcription from RNA polymerase II promoter molecular_function cellular_component BY4739 [1-1] A 3 YAL067C SEO1 putative permease SEO1 Putative permease; member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide other other membrane BY4739 [1-1] A 4 YAL066W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4739 [1-1] A 5 YAL065C - - Putative protein of unknown function; shows sequence similarity to FLO1 and other flocculins biological_process molecular_function cellular_component BY4739 [1-1] A 6 - cellular_component cellular_component cellular_component - - - - [1-1] A 7 - - - - - - - - [1-1] A 8 - - - - - - - - [1-1] A 9 YAL062W GDH3 glutamate dehydrogenase (NADP(+)) GDH3|FUN51 NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication cellular amino acid metabolic process oxidoreductase activity nucleus, mitochondrion, cytoplasm BY4739 [1-1] A 10 YAL061W BDH2 putative dehydrogenase BDH2 Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 biological_process oxidoreductase activity nucleus, cytoplasm BY4742 [1-1] A 11 YAL060W BDH1 (R,R)-butanediol dehydrogenase|BDH NAD-dependent (R,R)-butanediol dehydrogenase; catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source other oxidoreductase activity cytoplasm BY4739 [1-1] A 12 YAL059W ECM1 - Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm ribosomal subunit export from nucleus, nuclear transport molecular_function nucleus, nucleolus BY4739 [1-1] B 1 YAL058W CNE1 calnexin|FUN48 Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast response to chemical, proteolysis involved in cellular protein catabolic process, protein folding unfolded protein binding endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4739 [1-1] B 2 - endomembrane system, membrane, endoplasmic reticulum, cytoplasm endomembrane system, membrane, endoplasmic reticulum, cytoplasm endomembrane system, membrane, endoplasmic reticulum, cytoplasm - - - - [1-1] B 3 YAL056W GPB2 KRH1 Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication meiotic cell cycle, response to chemical, invasive growth in response to glucose limitation, sporulation, pseudohyphal growth, transcription from RNA polymerase II promoter, signaling enzyme regulator activity, signal transducer activity mitochondrion, membrane, cytoplasm, plasma membrane BY4739 [1-1] B 4 YAL055W PEX22 ubiquitin-protein transferase activating protein PEX22|YAF5 Putative peroxisomal membrane protein; required for import of peroxisomal proteins; functionally complements a Pichia pastoris pex22 mutation protein targeting, transmembrane transport, peroxisome organization, regulation of protein modification process, protein modification by small protein conjugation or removal, signaling enzyme regulator activity peroxisome, membrane, cytoplasm BY4739 [1-1] B 5 - peroxisome, membrane, cytoplasm peroxisome, membrane, cytoplasm peroxisome, membrane, cytoplasm - - - - [1-1] B 6 YAL053W FLC2 flavin adenine dinucleotide transporter FLC2|HUF2 Putative calcium channel involved in calcium release under hypotonic stress; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication transmembrane transport, ion transport, nucleobase-containing compound transport, lipid metabolic process, protein folding, cell wall organization or biogenesis, response to osmotic stress transmembrane transporter activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4739 [1-1] B 7 YAL051W OAF1 oleate-activated transcription factor OAF1|YAF1 Oleate-activated transcription factor; subunit of a heterodimeric complex with Pip2p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; regulates chromatin silencing at telomeres; involved in diauxic shift; OAF1 has a paralog, PIP2, that arose from the whole genome duplication response to chemical, lipid metabolic process, chromatin organization, transcription from RNA polymerase II promoter, monocarboxylic acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus BY4739 [1-1] B 8 YAL049C AIM2 protein AIM2 Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor biological_process molecular_function cytoplasm BY4739 [1-1] B 9 - cytoplasm cytoplasm cytoplasm - - - - [1-1] B 10 - - - - - - - - [1-1] B 11 - - - - - - - - [1-1] B 12 YAL045C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A biological_process molecular_function cellular_component BY4739 [1-1] C 1 YAL044C GCV3 glycine decarboxylase subunit H H subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4739 [1-1] C 2 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [1-1] C 3 YAL042W ERV46 FUN9 Protein localized to COPII-coated vesicles; forms a complex with Erv41p; involved in the membrane fusion stage of transport Golgi vesicle transport molecular_function membrane, cytoplasmic vesicle, endomembrane system, endoplasmic reticulum, cytoplasm, Golgi apparatus BY4739 [1-1] C 4 YAL043C-a membrane, cytoplasmic vesicle, endomembrane system, endoplasmic reticulum, cytoplasm, Golgi apparatus membrane, cytoplasmic vesicle, endomembrane system, endoplasmic reticulum, cytoplasm, Golgi apparatus membrane, cytoplasmic vesicle, endomembrane system, endoplasmic reticulum, cytoplasm, Golgi apparatus - - - BY4739 [1-1] C 5 - - - - - - - - [1-1] C 6 YAL040C CLN3 cyclin CLN3|WHI1|FUN10|DAF1 G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote G1 to S phase transition; plays a role in regulating transcription of other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis; acetyl-CoA induces CLN3 transcription in response to nutrient repletion to promote cell-cycle entry; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1 vacuole organization, mitotic cell cycle, protein phosphorylation, organelle fusion, membrane fusion, regulation of protein modification process, regulation of cell cycle enzyme regulator activity nucleus BY4739 [1-1] C 7 YAL039C CYC3 holocytochrome c synthase CYC3|CCHL Cytochrome c heme lyase (holocytochrome c synthase); attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in mitochondrial intermembrane space; human homolog HCCS implicated in microphthalmia with linear skin defects (MLS), and can complement yeast null mutant protein complex biogenesis lyase activity mitochondrial envelope, mitochondrion, cytoplasm BY4739 [1-1] C 8 - mitochondrial envelope, mitochondrion, cytoplasm mitochondrial envelope, mitochondrion, cytoplasm mitochondrial envelope, mitochondrion, cytoplasm - - - - [1-1] C 9 YAL037W - - Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4739 [1-1] C 10 YAL036C RBG1 GTP-binding protein RBG1|FUN11 Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid cytoplasmic translation, response to starvation ion binding ribosome, cytoplasm BY4739 [1-1] C 11 YAL035W FUN12 translation initiation factor eIF5B|eIF5B|yIF2 Translation initiation factor eIF5B; GTPase that promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2 cytoplasmic translation, translational initiation, regulation of translation, rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly RNA binding, GTPase activity, hydrolase activity, ion binding, translation factor activity, RNA binding ribosome, mitochondrion, cytoplasm BY4739 [1-1] C 12 - ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm - - - - [1-1] D 1 - - - - - - - - [1-1] D 2 YAL034C FUN19 - Non-essential protein of unknown function; expression induced in response to heat stress; FUN19 has a paralog, YOR338W, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4739 [1-1] D 3 - cellular_component cellular_component cellular_component - - - - [1-1] D 4 - - - - - - - - [1-1] D 5 YAL031C GIP4 protein phosphatase regulator GIP4|FUN21 Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; protein overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate regulation of protein modification process, protein dephosphorylation, chromosome segregation enzyme binding, enzyme regulator activity cytoplasm BY4739 [1-1] D 6 YAL030W SNC1 SNAP receptor SNC1 Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication organelle fusion, vesicle organization, membrane fusion, Golgi vesicle transport, exocytosis, endocytosis other cytoplasmic vesicle, endomembrane system, cellular bud, membrane, site of polarized growth, Golgi apparatus, cytoplasm, plasma membrane BY4739 [1-1] D 7 YAL029C MYO4 myosin 4|SHE1|FUN22 Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication organelle inheritance cytoskeletal protein binding, hydrolase activity cellular bud, site of polarized growth, cytoskeleton, mitochondrion, cytoplasm BY4739 [1-1] D 8 YAL028W FRT2 HPH2 Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication response to osmotic stress molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4739 [1-1] D 9 YAL027W SAW1 DNA-binding protein SAW1 5'- and 3'-flap DNA binding protein; recruits Rad1p-Rad10p to single-strand annealing intermediates with 3' non-homologous tails for removal during double-strand break repair; complexes with Rad1p-Rad10p and stimulates its endonuclease activity; green fluorescent protein (GFP)-fusion protein localizes to the nucleus DNA repair, regulation of DNA metabolic process, cellular response to DNA damage stimulus DNA binding nucleus BY4739 [1-1] D 10 YAL026C DRS2 aminophospholipid-translocating P4-type ATPase DRS2|SWA3|FUN38 Trans-golgi network aminophospholipid translocase (flippase); maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation, endocytosis, protein trafficking between the Golgi and endosomal system and the cellular response to mating pheromone; autoinhibited by its C-terminal tail; localizes to the trans-Golgi network; mutations in human homolog ATP8B1 result in liver disease response to chemical, ribosome assembly, Golgi vesicle transport, lipid transport, conjugation, ion transport, organelle assembly, ribosomal small subunit biogenesis, endosomal transport, endocytosis hydrolase activity, ATPase activity Golgi apparatus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4739 [1-1] D 11 - Golgi apparatus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm Golgi apparatus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm Golgi apparatus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm - - - - [1-1] D 12 - - - - - - - - [1-1] E 1 YAL023C PMT2 dolichyl-phosphate-mannose-protein mannosyltransferase PMT2|FUN25 Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication response to chemical, proteolysis involved in cellular protein catabolic process, protein glycosylation, signaling transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4739 [1-1] E 2 YAL022C FUN26 nucleoside transmembrane transporter FUN26 High affinity, broad selectivity, nucleoside/nucleobase transporter; vacuolar membrane localized transporter which may regulate the balance of nicotinamide riboside (NmR) levels between the cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis; equilibrative nucleoside transporter (ENT) family member transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4739 [1-1] E 3 YAL021C CCR4 CCR4-NOT core exoribonuclease subunit CCR4|NUT21|FUN27 Component of the CCR4-NOT transcriptional complex; CCR4-NOT is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening mitotic cell cycle, DNA replication, RNA catabolic process, regulation of DNA metabolic process, transcription from RNA polymerase II promoter, regulation of cell cycle, DNA-templated transcription, elongation hydrolase activity, nuclease activity nucleus, cytoplasm BY4739 [1-1] E 4 YAL020C ATS1 KTI13|FUN28 Protein required for modification of wobble nucleosides in tRNA; acts with Elongator complex, Kti11p, and Kti12p; has a potential role in regulatory interactions between microtubules and the cell cycle; forms a stable heterodimer with Kti11p tRNA processing, cell budding, cytoskeleton organization, RNA modification molecular_function cytoplasm BY4739 [1-1] E 5 YAL019W FUN30 DNA-dependent ATPase FUN30 Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate DNA repair, chromatin organization, cellular response to DNA damage stimulus, transcription from RNA polymerase II promoter chromatin binding, hydrolase activity, DNA binding, ATPase activity nucleus, chromosome, mitochondrion, cytoplasm BY4739 [1-1] E 6 YAL018C LDS1 - Protein Involved in spore wall assembly; localizes to lipid droplets found on or outside of the prospore membrane; shares similarity with Lds2p and Rrt8p, and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function cell wall, membrane BY4739 [1-1] E 7 YAL017W PSK1 serine/threonine protein kinase PSK1|FUN31 PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication carbohydrate metabolic process, protein phosphorylation, generation of precursor metabolites and energy kinase activity, transferase activity cytoplasm BY4739 [1-1] E 8 - cytoplasm cytoplasm cytoplasm - - - - [1-1] E 9 YAL015C NTG1 bifunctional N-glycosylase/AP lyase NTG1|ogg2|SCR1|FUN33 DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; required for maintaining mitochondrial genome integrity; NTG1 has a paralog, NTG2, that arose from the whole genome duplication DNA repair, response to chemical, DNA replication, mitochondrion organization, regulation of organelle organization, cellular response to DNA damage stimulus, response to oxidative stress, regulation of DNA metabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds, lyase activity, nuclease activity nucleus, mitochondrion, cytoplasm BY4739 [1-1] E 10 YAL014C SYN8 syntaxin|SLT2|UIP2 Endosomal SNARE related to mammalian syntaxin 8 other other cytoplasmic vesicle, endomembrane system, cytoplasm BY4739 [1-1] E 11 YAL013W DEP1 Rpd3L histone deacetylase complex subunit DEP1|FUN54 Component of the Rpd3L histone deacetylase complex; required for diauxic shift-induced histone H2B deposition onto rDNA genes; transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation response to heat, carbohydrate metabolic process, chromatin organization, DNA replication, histone modification, lipid metabolic process, regulation of DNA metabolic process, transcription from RNA polymerase II promoter molecular_function nucleus, chromosome BY4742 [1-1] E 12 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [1-1] F 1 YAL011W SWC3 SWC1 Protein of unknown function; component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae chromatin organization molecular_function nucleus, chromosome, mitochondrion, cytoplasm BY4739 [1-1] F 2 YAL010C MDM10 FUN37 Subunit of both the ERMES and the SAM complex; component of ERMES complex which acts as a molecular tether between the mitochondria and the ER, necessary for efficient phospholipid exchange between organelles and for mitophagy; SAM/TOB complex component that functions in the assembly of outer membrane beta-barrel proteins; involved in mitochondrial inheritance and morphology; ERMES complex is often co-localized with peroxisomes and concentrated areas of pyruvate dehydrogenase protein targeting, mitochondrion organization, transmembrane transport, ion transport, protein complex biogenesis, lipid transport molecular_function endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm BY4739 [1-1] F 3 YAL009W SPO7 Nem1-Spo7 phosphatase regulatory subunit SPO7 Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation protein dephosphorylation, lipid metabolic process, nucleus organization phosphatase activity, hydrolase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4739 [1-1] F 4 YAL008W FUN14 MCP3 Integral mitochondrial outer membrane (MOM) protein; dosage suppressor of an MDM10 null that reduces ERMES-related phenotypes, such as alterations in mitochondrial morphology, protein complex assembly, and lipid profile; dosage suppressor of MDM12, MDM34, and MMM1 null mutant growth defects; novel mechanism of MOM import involving Tom70p, the TOM complex, and the TIM23 complex, requiring mitochondrial membrane potential and processing by the IMP complex for correct biogenesis mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4739 [1-1] F 5 YAL007C ERP2 - Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP2 has a paralog, ERP4, that arose from the whole genome duplication Golgi vesicle transport molecular_function cytoplasmic vesicle, endomembrane system, endoplasmic reticulum, cytoplasm BY4739 [1-1] F 6 YAL004W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C biological_process molecular_function cellular_component BY4739 [1-1] F 7 YAL005C SSA1 Hsp70 family ATPase SSA1|YG100 ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; required for ubiquitin-dependent degradation of short-lived proteins; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, cell wall; 98% identical to paralog Ssa2p with different functional specificity in propagation of yeast [URE3] prions, vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils cytoplasmic translation, transmembrane transport, proteolysis involved in cellular protein catabolic process, protein targeting, mitochondrion organization, protein folding, protein modification by small protein conjugation or removal, nuclear transport RNA binding, hydrolase activity, unfolded protein binding, ATPase activity nucleus, vacuole, cell wall, plasma membrane, membrane, cytoplasm BY4739 [1-1] F 8 - nucleus, vacuole, cell wall, plasma membrane, membrane, cytoplasm nucleus, vacuole, cell wall, plasma membrane, membrane, cytoplasm nucleus, vacuole, cell wall, plasma membrane, membrane, cytoplasm - - - - [1-1] F 9 YAL002W VPS8 CORVET complex membrane-binding subunit VPS8|VPL8|VPT8|FUN15 Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif protein targeting, endosomal transport enzyme binding membrane, cytoplasmic vesicle, endomembrane system, cytoplasm BY4739 [1-1] F 10 - membrane, cytoplasmic vesicle, endomembrane system, cytoplasm membrane, cytoplasmic vesicle, endomembrane system, cytoplasm membrane, cytoplasmic vesicle, endomembrane system, cytoplasm - - - - [1-1] F 11 YAR002W NUP60 FG-nucleoporin NUP60 FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; relocalizes to the cytosol in response to hypoxia; both NUP1 and NUP60 are homologous to human NUP153 response to heat, peptidyl-amino acid modification, chromatin organization, DNA repair, protein targeting, cellular response to DNA damage stimulus, nucleobase-containing compound transport, regulation of protein modification process, protein modification by small protein conjugation or removal, nuclear transport ion binding, lipid binding, structural molecule activity nucleus, endomembrane system, membrane, cytoplasm BY4739 [1-1] F 12 YAR003W SWD1 COMPASS subunit protein SWD1|SAF49|CPS50|FUN16 Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 peptidyl-amino acid modification, telomere organization, protein alkylation, chromatin organization, histone modification transferase activity, methyltransferase activity nucleus BY4739 [1-1] G 1 - nucleus nucleus nucleus - - - - [1-1] G 2 - - - - - - - - [1-1] G 3 - - - - - - - - [1-1] G 4 - - - - - - - - [1-1] G 5 YAR014C BUD14 protein phosphatase regulator BUD14 Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; forms a complex with Kel1p and Kel2p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress cell morphogenesis, cytoskeleton organization, regulation of organelle organization, cytokinesis, regulation of transport, protein complex biogenesis, regulation of cell cycle enzyme regulator activity nucleus, cellular bud, site of polarized growth, cytoplasm BY4739 [1-1] G 6 YAR015W ADE1 phosphoribosylaminoimidazolesuccinocarboxamide synthase N-succinyl-5-aminoimidazole-4-carboxamide ribotide synthetase; required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress nucleobase-containing small molecule metabolic process ligase activity nucleus, cytoplasm BY4739 [1-1] G 7 YAR018C KIN3 serine/threonine protein kinase KIN3|NPK1|FUN52 Nonessential serine/threonine protein kinase; possible role in DNA damage response; influences tolerance to high levels of ethanol protein phosphorylation, chromosome segregation kinase activity, transferase activity cellular_component BY4739 [1-1] G 8 - cellular_component cellular_component cellular_component - - - - [1-1] G 9 YAR020C PAU7 seripauperin PAU7 Member of the seripauperin multigene family; active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme biological_process molecular_function cellular_component BY4739 [1-1] G 10 YAR023C - DUP240 family protein Putative integral membrane protein; member of DUP240 gene family biological_process molecular_function cellular_component BY4739 [1-1] G 11 YAR027W UIP3 DUP240 family protein UIP3 Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family biological_process molecular_function nucleus, membrane, endomembrane system, vacuole, cytoplasm BY4739 [1-1] G 12 YAR028W - DUP240 family protein Putative integral membrane protein; member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function membrane, vacuole, cytoplasm BY4739 [1-1] H 1 YAR029W - DUP240 family protein Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function cytoplasm BY4742 [1-1] H 2 YAR031W PRM9 pheromone-regulated DUP240 family protein PRM9 Pheromone-regulated protein; contains 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family; PRM9 has a paralog, PRM8, that arose from a segmental duplication biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, plasma membrane, cytoplasm BY4739 [1-1] H 3 YAR030C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 biological_process molecular_function cellular_component BY4742 [1-1] H 4 - cellular_component cellular_component cellular_component - - - - [1-1] H 5 YAR035W YAT1 carnitine O-acetyltransferase YAT1 Outer mitochondrial carnitine acetyltransferase; minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated other transferase activity mitochondrion, cytoplasm BY4739 [1-1] H 6 YAR037W mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - BY4739 [1-1] H 7 YAR040C - - - - - - BY4739 [1-1] H 8 YAR042W SWH1 oxysterol-binding protein related protein SWH1|OSH1|YAR044W Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats and FFAT motif; interacts with ER anchor Scs2p at the nucleus-vacuole junction; regulated by sterol binding; SWH1 has a paralog, OSH2, that arose from the whole genome duplication lipid transport, exocytosis, endocytosis lipid binding, ion binding nucleus, cytoplasmic vesicle, endomembrane system, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4739 [1-1] H 9 YAR043C nucleus, cytoplasmic vesicle, endomembrane system, endoplasmic reticulum, Golgi apparatus, cytoplasm nucleus, cytoplasmic vesicle, endomembrane system, endoplasmic reticulum, Golgi apparatus, cytoplasm nucleus, cytoplasmic vesicle, endomembrane system, endoplasmic reticulum, Golgi apparatus, cytoplasm - - - BY4739 [1-1] H 10 YAR044W - - - - - - BY4739 [1-1] H 11 YAR047C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4739 [1-1] H 12 - cellular_component cellular_component cellular_component - - - - [2-1] A 1 YBL001C ECM15 - Non-essential protein of unknown function; likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis biological_process molecular_function nucleus, cytoplasm BY4742 [2-1] A 2 YBL002W HTB2 histone H2B Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation chromatin organization DNA binding nucleus, chromosome BY4742 [2-1] A 3 YBL003C HTA2 histone H2A|H2A2 Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p DNA repair, chromatin organization, cellular response to DNA damage stimulus DNA binding nucleus, chromosome BY4742 [2-1] A 4 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [2-1] A 5 YBL005W PDR3 drug-responsive transcription factor PDR3|TPE2|AMY2 Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting PDRE sites (PDR responsive elements); has a role in response to drugs and organic solvents; post-translationally up-regulated in cells lacking functional mitochondrial genome; involved in diauxic shift; relative distribution to nucleus increases upon DNA replication stress; APCC(Cdh1) substrate response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity nucleus, cytoplasm BY4742 [2-1] A 6 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [2-1] A 7 - - - - - - - - [2-1] A 8 YBL006C LDB7 RSC14 Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions chromatin organization, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation ATPase activity, hydrolase activity nucleus BY4742 [2-1] A 9 YBL007C SLA1 cytoskeletal protein-binding protein SLA1 Cytoskeletal protein binding protein; required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains cytoskeleton organization, protein complex biogenesis, endocytosis protein binding, bridging, ubiquitin-like protein binding nucleus, cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [2-1] A 10 YBL008W HIR1 - Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores chromatin organization, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation chromatin binding, DNA binding, transcription factor activity, protein binding nucleus, chromosome BY4742 [2-1] A 11 YBL009W ALK2 protein kinase ALK2 Protein kinase; along with its paralog, ALK1, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of cell polarity factors in mitosis, proper localization of formins and polarity factors, and survival in cells that activate spindle assembly checkpoint; phosphorylated in response to DNA damage; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins mitotic cell cycle, meiotic cell cycle, protein phosphorylation, organelle fission kinase activity, transferase activity cellular_component BY4742 [2-1] A 12 YBL010C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles biological_process molecular_function cytoplasmic vesicle, cytoplasm BY4742 [2-1] B 1 YBL011W SCT1 bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase SCT1|GAT2 Glycerol 3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; dual substrate-specific acyltransferase of the glycerolipid biosynthesis pathway; prefers 16-carbon fatty acids; similar to Gpt2p; gene is constitutively transcribed lipid metabolic process transferase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-1] B 2 YBL012C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [2-1] B 3 YBL013W FMT1 methionyl-tRNA formyltransferase Methionyl-tRNA formyltransferase; catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate tRNA processing, RNA modification transferase activity mitochondrion, cytoplasm BY4742 [2-1] B 4 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [2-1] B 5 YBL015W ACH1 acetyl-CoA hydrolase Protein with CoA transferase activity; particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth monocarboxylic acid metabolic process transferase activity, hydrolase activity mitochondrion, cytoplasm BY4742 [2-1] B 6 YBL016W FUS3 mitogen-activated serine/threonine-protein kinase FUS3|DAC2 Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its; inhibits recruitment of Ste5p, Cdc42p-mediated asymmetry and mating morphogenesis response to chemical, regulation of transport, conjugation, invasive growth in response to glucose limitation, protein phosphorylation, transposition, regulation of protein modification process, nuclear transport, regulation of cell cycle, signaling signal transducer activity, kinase activity, transferase activity nucleus, mitochondrion, site of polarized growth, cytoplasm BY4742 [2-1] B 7 YBL017C PEP1 type I sorting receptor|VPT1|VPS10 Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments protein targeting other membrane, endomembrane system, vacuole, cytoplasm, Golgi apparatus BY4742 [2-1] B 8 - membrane, endomembrane system, vacuole, cytoplasm, Golgi apparatus membrane, endomembrane system, vacuole, cytoplasm, Golgi apparatus membrane, endomembrane system, vacuole, cytoplasm, Golgi apparatus - - - - [2-1] B 9 YBL019W APN2 DNA-(apurinic or apyrimidinic site) lyase APN2|ETH1 Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII DNA repair, cellular response to DNA damage stimulus lyase activity, nuclease activity, hydrolase activity nucleus BY4742 [2-1] B 10 - nucleus nucleus nucleus - - - - [2-1] B 11 YBL021C HAP3 - Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding response to chemical, transcription from RNA polymerase II promoter, generation of precursor metabolites and energy, cellular respiration nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [2-1] B 12 YBL022C PIM1 ATP-dependent Lon protease PIM1|LON1 ATP-dependent Lon protease; involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria proteolysis involved in cellular protein catabolic process, protein complex biogenesis peptidase activity, ATPase activity, hydrolase activity mitochondrion, cytoplasm BY4742 [2-1] C 1 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [2-1] C 2 YBL024W NCL1 tRNA (cytosine-C5-)-methyltransferase|TRM4 S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase; methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 response to chemical, tRNA processing, response to oxidative stress, RNA modification RNA binding, transferase activity, methyltransferase activity nucleus BY4742 [2-1] C 3 YBL025W RRN10 - Protein involved in promoting high level transcription of rDNA; subunit of UAF (upstream activation factor) for RNA polymerase I transcription from RNA polymerase I promoter nucleic acid binding transcription factor activity, DNA binding nucleus, nucleolus BY4742 [2-1] C 4 - nucleus, nucleolus nucleus, nucleolus nucleus, nucleolus - - - - [2-1] C 5 YBL027W RPL19B eL19|ribosomal 60S subunit protein L19B|L19e|rpl5L|YL14|L23B|L19B Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [2-1] C 6 YBL028C - - Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis biological_process molecular_function nucleus, nucleolus, ribosome, cytoplasm BY4742 [2-1] C 7 YBL029W - - Non-essential protein of unknown function biological_process molecular_function nucleus, cytoplasm BY4742 [2-1] C 8 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [2-1] C 9 YBL031W SHE1 - Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function mitotic cell cycle, organelle fission, cytoskeleton organization, chromosome segregation cytoskeletal protein binding nucleus, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [2-1] C 10 YBL032W HEK2 KHD1 RNA binding protein involved in asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K telomere organization RNA binding, mRNA binding nucleus, chromosome, cytoplasm BY4742 Slow Growing [2-1] C 11 YBL033C RIB1 GTP cyclohydrolase II GTP cyclohydrolase II; catalyzes the first step of the riboflavin biosynthesis pathway vitamin metabolic process hydrolase activity nucleus, cytoplasm BY4742 [2-1] C 12 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [2-1] D 1 - - - - - - - - [2-1] D 2 YBL036C - - Putative non-specific single-domain racemase; based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS biological_process ion binding cellular_component BY4742 [2-1] D 3 YBL037W APL3 - Alpha-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport other molecular_function cytoplasmic vesicle, endomembrane system, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane BY4742 [2-1] D 4 YBL038W MRPL16 RML16|mitochondrial 54S ribosomal protein YmL47|YmL47 Mitochondrial ribosomal protein of the large subunit; homologous to bacterial L16 ribosomal protein; synthetic lethality with hac1 mutation suggests a possible role in synthesis of precursors for protein glycosylation mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [2-1] D 5 YBL039C URA7 CTP synthase URA7 Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication lipid metabolic process, nucleobase-containing small molecule metabolic process ligase activity vacuole, cytoplasm BY4742 [2-1] D 6 - vacuole, cytoplasm vacuole, cytoplasm vacuole, cytoplasm - - - - [2-1] D 7 - - - - - - - - [2-1] D 8 YBL042C FUI1 uridine permease High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [2-1] D 9 YBL043W ECM13 - Non-essential protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; ECM13 has a paralog, YJR115W, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4742 [2-1] D 10 YBL044W - - Putative protein of unknown function; YBL044W is not an essential protein biological_process molecular_function cellular_component BY4742 [2-1] D 11 YBL045C COR1 ubiquinol--cytochrome-c reductase subunit COR1|QCR1 Core subunit of the ubiquinol-cytochrome c reductase complex; the ubiquinol-cytochrome c reductase complex (bc1 complex) is a component of the mitochondrial inner membrane electron transport chain generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-1] D 12 YBL046W PSY4 HSM6 Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 protein dephosphorylation, cellular response to DNA damage stimulus, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm BY4742 [2-1] E 1 YBL047C EDE1 BUD15 Scaffold protein involved in the formation of early endocytic sites; putative regulator of cytokinesis; homo-oligomerization is required for localization to and organization of endocytic sites; has a network of interactions with other endocytic proteins; binds membranes in a ubiquitin-dependent manner; may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death; homolog of mammalian Eps15 response to chemical, cytokinesis, signaling, regulation of cell cycle, endocytosis ubiquitin-like protein binding cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [2-1] E 2 YBL048W RRT1 - Protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data biological_process molecular_function membrane BY4742 [2-1] E 3 YBL049W MOH1 - Protein of unknown function, essential for stationary phase survival; not required for growth on nonfermentable carbon sources; possibly linked with vacuolar transport biological_process molecular_function cellular_component BY4742 [2-1] E 4 - cellular_component cellular_component cellular_component - - - - [2-1] E 5 YBL051C PIN4 MDT1 Protein involved in G2/M phase progression and response to DNA damage; interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage mitotic cell cycle, cellular response to DNA damage stimulus, regulation of cell cycle RNA binding, mRNA binding cytoplasm BY4742 "Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p" [2-1] E 6 YBL052C SAS3 KAT6 Histone acetyltransferase catalytic subunit of NuA3 complex; acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity nucleus BY4742 [2-1] E 7 YBL053W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [2-1] E 8 YBL054W TOD6 PBF1 PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; activated in stochastic pulses of nuclear localization transcription from RNA polymerase II promoter DNA binding nucleus, chromosome, cytoplasm BY4742 [2-1] E 9 YBL055C - 3'-5'-exodeoxyribonuclease|Tat-D 3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases response to chemical, response to oxidative stress nuclease activity, hydrolase activity cytoplasm BY4742 [2-1] E 10 YBL056W PTC3 type 2C protein phosphatase PTC3 Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication protein phosphorylation, protein dephosphorylation, regulation of protein modification process, signaling, response to osmotic stress, regulation of cell cycle phosphatase activity, hydrolase activity nucleus, cytoplasm BY4742 [2-1] E 11 YBL057C PTH2 aminoacyl-tRNA hydrolase One of two mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth; see also PTH1 proteolysis involved in cellular protein catabolic process hydrolase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-1] E 12 YBL058W SHP1 protein phosphatase regulator SHP1|UBX1 UBX domain-containing substrate adaptor for Cdc48p; ubiquitin regulatory X domain-containing protein that acts as a substrate recruiting cofactor for Cdc48p; positively regulates Glc7p PPase activity to promote growth and mitotic progression in complex with Cdc48p; ubiquitinated protein interactor involved in ER-associated degradation (ERAD); regulated by nuclear Ub-dependent degradation (INMAD pathway) independent of the Asi and Doa10 complexes; homolog of human p47 (NSFL1C) vacuole organization, meiotic cell cycle, carbohydrate metabolic process, response to chemical, sporulation, organelle assembly, proteolysis involved in cellular protein catabolic process, generation of precursor metabolites and energy, chromosome segregation enzyme regulator activity nucleus, cytoplasm BY4742 Slow Growing [2-1] F 1 YBL059W - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication biological_process molecular_function mitochondrion, cytoplasm BY4742 [2-1] F 2 YBL060W YEL1 Arf family guanine nucleotide exchange factor YEL1 Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip lipid metabolic process guanyl-nucleotide exchange factor activity cellular bud, site of polarized growth, cytoplasm BY4742 [2-1] F 3 YBL061C SKT5 CSD4|CHS4|CAL2 Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication carbohydrate metabolic process, cell wall organization or biogenesis enzyme regulator activity cellular bud, cell cortex, site of polarized growth, cytoskeleton, cytoplasm BY4742 [2-1] F 4 YBL062W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [2-1] F 5 YBL063W KIP1 CIN9 Kinesin-related motor protein; required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions mitotic cell cycle, organelle fission, cytoskeleton organization, organelle assembly, chromosome segregation hydrolase activity, structural molecule activity nucleus, cytoskeleton, microtubule organizing center BY4742 [2-1] F 6 YBL064C PRX1 thioredoxin peroxidase PRX1 Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress response to chemical, response to oxidative stress oxidoreductase activity mitochondrion, cytoplasm BY4742 Mitochondrial peroxiredoxin (1-Cys Prx) with thioredoxin peroxidase activity [2-1] F 7 YBL065W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene biological_process molecular_function cellular_component BY4742 [2-1] F 8 YBL066C SEF1 - Putative transcription factor; has homolog in Kluyveromyces lactis biological_process molecular_function cellular_component BY4742 [2-1] F 9 YBL067C UBP13 ubiquitin-specific protease UBP13 Ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication other peptidase activity, hydrolase activity cellular_component BY4742 [2-1] F 10 YBL068W PRS4 ribose phosphate diphosphokinase subunit PRS4 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP; which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication; a missense mutation in the conserved residue R196 of its human homolog PRPS1 is pathogenic cell wall organization or biogenesis transferase activity cytoplasm BY4742 [2-1] F 11 YBL069W AST1 - Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication protein targeting molecular_function cytoplasmic vesicle, endomembrane system, membrane, Golgi apparatus, cytoplasm, plasma membrane BY4742 [2-1] F 12 YBL070C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [2-1] G 1 YBL071C - - Putative protein of unknown function; conserved among S. cerevisiae strains; YBL071C is not an essential gene biological_process molecular_function cellular_component BY4742 [2-1] G 2 YBL072C RPS8A eS8|ribosomal 40S subunit protein S8A|S8e|rp19|YS9|S8A|S14A Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [2-1] G 3 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [2-1] G 4 - - - - - - - - [2-1] G 5 YBL075C SSA3 Hsp70 family ATPase SSA3|YG106 ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication protein targeting, transmembrane transport, proteolysis involved in cellular protein catabolic process, protein folding hydrolase activity, unfolded protein binding, ATPase activity cytoplasm BY4742 [2-1] G 6 - cytoplasm cytoplasm cytoplasm - - - - [2-1] G 7 - - - - - - - - [2-1] G 8 YBL078C ATG8 ubiquitin-like protein ATG8|AUT7|CVT5|APG8 Component of autophagosomes and Cvt vesicles; regulator of Atg1p, targets it to autophagosomes; binds the Atg1p-Atg13p complex, triggering its vacuolar degradation; unique ubiquitin-like protein whose conjugation target is lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis vacuole organization, protein targeting, mitochondrion organization, organelle assembly, Golgi vesicle transport, membrane fusion other vacuole, membrane, cytoplasm BY4742 [2-1] G 9 YBL079W NUP170 NLE3 Subunit of inner ring of nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; interacts with genomic regions that contain ribosomal protein and subtelomeric genes, where it functions in nucleosome positioning and as a repressor of transcription; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication protein targeting, chromatin organization, protein complex biogenesis, chromosome segregation, nuclear transport, nucleus organization chromatin binding, structural molecule activity nucleus, endomembrane system, membrane BY4742 [2-1] G 10 YBL080C PET112 glutamyl-tRNA(Gln) amidotransferase subunit PET112 Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog mitochondrial translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm BY4742 [2-1] G 11 YBL081W - - Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport biological_process molecular_function cellular_component BY4742 [2-1] G 12 YBL082C ALG3 dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase|RHK1 Dolichol-P-Man dependent alpha(1-3) mannosyltransferase; involved in synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins; G353A missense mutation in human ortholog ALG3 implicated in carbohydrate deficient glycoprotein syndrome type IV, which is characterized by microcephaly, severe epilepsy, minimal psychomotor development, partial deficiency of sialic acids in serum glycoproteins; wild-type human ALG3 can complement yeast alg3 mutant protein glycosylation, lipid metabolic process transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [2-1] H 1 YBL083C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 biological_process molecular_function cellular_component BY4742 [2-1] H 2 - cellular_component cellular_component cellular_component - - - - [2-1] H 3 YBL085W BOI1 BOB1|GIN7 Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication cell budding lipid binding, ion binding cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane BY4742 [2-1] H 4 YBL086C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function cellular_component BY4742 [2-1] H 5 YBL087C RPL23A uL14|ribosomal 60S subunit protein L23A|L14|YL32|L23A|L17aA Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [2-1] H 6 YBL088C TEL1 DNA-binding protein kinase TEL1 Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; acts with Red1p and Mec1p to promote interhomolog recombination by phosphorylation of Hop1; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene DNA repair, telomere organization, protein phosphorylation, chromatin organization, cellular response to DNA damage stimulus, histone modification kinase activity, transferase activity, DNA binding nucleus, mitochondrion, cytoplasm BY4742 [2-1] H 7 YBL089W AVT5 amino acid transporter Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication other other membrane BY4742 "Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters" [2-1] H 8 YBL090W MRP21 mitochondrial 37S ribosomal protein MRP21|MRP50 Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences translational initiation, mitochondrial translation, mitochondrion organization structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm BY4742 [2-1] H 9 YBL091C MAP2 methionine aminopeptidase Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p protein maturation peptidase activity, hydrolase activity nucleus, cytoplasm BY4742 [2-1] H 10 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [2-1] H 11 YBL093C ROX3 SSX2|MED19|SSN7|NUT3 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme DNA-templated transcription, initiation, transcription from RNA polymerase II promoter transcription factor activity, protein binding nucleus BY4742 Slow Growing [2-1] H 12 YBL094C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W biological_process molecular_function cellular_component BY4742 [2-2] A 1 YBL095W MRX3 - Protein that associates with mitochondrial ribosome; likely functions in cristae junction formation; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-2] A 2 YBL096C - - Non-essential protein of unknown function biological_process molecular_function cellular_component BY4742 [2-2] A 3 - cellular_component cellular_component cellular_component - - - - [2-2] A 4 YBL098W BNA4 kynurenine 3-monooxygenase Kynurenine 3-monooxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease cofactor metabolic process, cellular amino acid metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-2] A 5 YBL099W ATP1 F1F0 ATP synthase subunit alpha Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated; N-terminally propionylated in vivo transmembrane transport, ion transport, nucleobase-containing small molecule metabolic process hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-2] A 6 YBL100C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the 5' end of ATP1 biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-2] A 7 YBL101C ECM21 ART2 Protein involved in regulating endocytosis of plasma membrane proteins; identified as a substrate for ubiquitination by Rsp5p and deubiquitination by Ubp2p; promoter contains several Gcn4p binding elements; ECM21 has a paralog, CSR2, that arose from the whole genome duplication endocytosis enzyme binding cytoplasm BY4742 [2-2] A 8 - cytoplasm cytoplasm cytoplasm - - - - [2-2] A 9 - - - - - - - - [2-2] A 10 YBL102W SFT2 - Tetra-spanning membrane protein found mostly in the late Golgi; non-essential; can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 endosomal transport molecular_function membrane, endomembrane system, cytoplasm, Golgi apparatus BY4742 [2-2] A 11 YBL103C RTG3 - bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways; forms a complex with another bHLH/Zip protein, Rtg1p, to activate the pathways; target of Hog1p response to chemical, protein targeting, mitochondrion organization, transcription from RNA polymerase II promoter, nuclear transport, signaling nucleic acid binding transcription factor activity, DNA binding, transcription factor binding nucleus, cytoplasm BY4742 [2-2] A 12 YBL104C SEA4 YBL103C-A Subunit of SEACAT, a subcomplex of the SEA complex; Sea4p, along with Rtc1p and Mtc5p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; contains an N-terminal beta-propeller fold and a C-terminal RING motif signaling molecular_function membrane, vacuole, cytoplasm BY4742 [2-2] B 1 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [2-2] B 2 YBL106C SRO77 putative Rab GTPase-binding protein SRO77|SNI2|SOP2 Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; regulates cell proliferation and colony development via the Rho1-Tor1 pathway; interacts with SNARE protein Sec9p; homolog of Drosophila lethal giant larvae tumor suppressor; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Golgi vesicle transport, exocytosis molecular_function cellular bud, membrane, site of polarized growth, plasma membrane BY4742 [2-2] B 3 YBL107C MIX23 MIC23 Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) biological_process molecular_function mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-2] B 4 YBR001C NTH2 alpha,alpha-trehalase NTH2 Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication carbohydrate metabolic process, oligosaccharide metabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds mitochondrion, cytoplasm BY4742 [2-2] B 5 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [2-2] B 6 YBR003W COQ1 trans-hexaprenyltranstransferase Hexaprenyl pyrophosphate synthetase; catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis cofactor metabolic process transferase activity mitochondrion, cytoplasm BY4742 [2-2] B 7 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [2-2] B 8 YBR005W RCR1 SSH6 Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication other molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-2] B 9 YBR006W UGA2 succinate-semialdehyde dehydrogenase (NAD(P)(+))|UGA5 Succinate semialdehyde dehydrogenase; involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm response to chemical, response to oxidative stress, monocarboxylic acid metabolic process, cellular amino acid metabolic process oxidoreductase activity cytoplasm BY4742 [2-2] B 10 YBR007C DSF2 - Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress biological_process molecular_function cellular bud, site of polarized growth BY4742 [2-2] B 11 YBR008C FLR1 - Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress response to chemical, transmembrane transport other nucleus, membrane, plasma membrane, cytoplasm BY4742 [2-2] B 12 YBR009C HHF1 histone H4 Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity peptidyl-amino acid modification, meiotic cell cycle, protein alkylation, chromatin organization, sporulation, histone modification, transcription from RNA polymerase II promoter DNA binding nucleus, chromosome BY4742 [2-2] C 1 YBR010W HHT1 histone H3|SIN2|BUR5 Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage meiotic cell cycle, DNA repair, chromatin organization, sporulation, cellular response to DNA damage stimulus DNA binding nucleus, chromosome BY4742 [2-2] C 2 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [2-2] C 3 YBR012C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by iron-regulated transcriptional activator Aft2p biological_process molecular_function cellular_component BY4742 [2-2] C 4 - cellular_component cellular_component cellular_component - - - - [2-2] C 5 - - - - - - - - [2-2] C 6 YBR013C - - Putative protein of unknown function; haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein; SWAT-GFP fusion protein localizes to the endoplasmic reticulum while mCherry fusion protein localizes to both the endoplasmic reticulum and vacuole biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-2] C 7 YBR014C GRX7 glutathione-disulfide reductase GRX7 Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication response to chemical, response to oxidative stress oxidoreductase activity endomembrane system, vacuole, membrane, Golgi apparatus, cytoplasm BY4742 [2-2] C 8 YBR015C MNN2 alpha-1,2-mannosyltransferase MNN2|LDB8|CRV4|TTP1 Alpha-1,2-mannosyltransferase; responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment protein glycosylation transferase activity, transferring glycosyl groups, transferase activity endomembrane system, cytoplasm, Golgi apparatus BY4742 [2-2] C 9 YBR016W - - Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication biological_process molecular_function cellular bud, membrane, plasma membrane BY4742 [2-2] C 10 - cellular bud, membrane, plasma membrane cellular bud, membrane, plasma membrane cellular bud, membrane, plasma membrane - - - - [2-2] C 11 YBR018C GAL7 UDP-glucose:hexose-1-phosphate uridylyltransferase Galactose-1-phosphate uridyl transferase; synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism; human homolog UGP2 can complement yeast null mutant carbohydrate metabolic process transferase activity, nucleotidyltransferase activity cytoplasm BY4742 [2-2] C 12 YBR019C GAL10 bifunctional UDP-glucose 4-epimerase/aldose 1-epimerase UDP-glucose-4-epimerase; catalyzes interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers; human homolog GALE implicated in galactosemia, can complement yeast null mutant carbohydrate metabolic process isomerase activity cytoplasm BY4742 [2-2] D 1 YBR020W GAL1 galactokinase Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; human homolog GALK2 complements yeast null mutant; GAL1 has a paralog, GAL3, that arose from the whole genome duplication carbohydrate metabolic process, response to chemical kinase activity, transferase activity cytoplasm BY4742 [2-2] D 2 YBR021W FUR4 uracil permease Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation transmembrane transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [2-2] D 3 YBR022W POA1 ADP-ribose 1''-phosphate phosphatase Phosphatase that is highly specific for ADP-ribose 1''-phosphate; a tRNA splicing metabolite; may have a role in regulation of tRNA splicing RNA splicing, tRNA processing phosphatase activity, hydrolase activity, hydrolase activity, acting on glycosyl bonds cellular_component BY4742 [2-2] D 4 YBR023C CHS3 chitin synthase CHS3|KTI2|DIT101|CSD2|CAL1 Chitin synthase III; catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan; contains overlapping di-leucine and di-acidic signals that mediate, respectively, intracellular trafficking by AP-1 and trafficking to plasma membrane by exomer complex; requires AP-3 complex for its intracellular retention meiotic cell cycle, carbohydrate metabolic process, sporulation, cell wall organization or biogenesis transferase activity, transferring glycosyl groups, transferase activity cytoplasmic vesicle, endomembrane system, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm BY4742 [2-2] D 5 YBR024W SCO2 putative thioredoxin peroxidase SCO2 Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication ion transport oxidoreductase activity mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-2] D 6 YBR025C OLA1 Obg-like ATPase P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; null mutant displays increased translation rate and increased readthrough of premature stop codons; protein abundance increases in response to hydrogen peroxide and to DNA replication stress biological_process ATPase activity, hydrolase activity cytoplasm BY4742 [2-2] D 7 YBR026C ETR1 MRF1'|MRF1 2-enoyl thioester reductase; member of the medium chain dehydrogenase/reductase family; localized to mitochondria, where it has a probable role in fatty acid synthesis; human MECR functionally complements the respiratory growth defect of the null mutant lipid metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, cellular respiration oxidoreductase activity mitochondrion, cytoplasm BY4742 [2-2] D 8 YBR027C - - Putative protein of unknown function; conserved among S. cerevisiae strains; YBR027C is not an essential gene biological_process molecular_function cellular_component BY4742 [2-2] D 9 YBR028C YPK3 putative protein kinase YPK3 AGC kinase; phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner; directly phosphorylated by TORC1; phosphorylates ribosomal protein Rps6a/b (S6), in a TORC-dependent manner; undergoes autophosphorylation peptidyl-amino acid modification, protein phosphorylation kinase activity, transferase activity cytoplasm BY4742 [2-2] D 10 - cytoplasm cytoplasm cytoplasm - - - - [2-2] D 11 YBR030W RKM3 protein-lysine N-methyltransferase Ribosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein; relocalizes to the cytosol in response to hypoxia peptidyl-amino acid modification, protein alkylation transferase activity, methyltransferase activity nucleus, cytoplasm BY4742 [2-2] D 12 YBR031W RPL4A uL4|ribosomal 60S subunit protein L4A|L4|rp2|YL2|L4A|L2A Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [2-2] E 1 YBR032W - - Putative protein of unknown function; conserved among S. cerevisiae strains; YBR032W is not an essential gene biological_process molecular_function cellular_component BY4742 [2-2] E 2 YBR033W EDS1 - Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication biological_process DNA binding cellular_component BY4742 [2-2] E 3 YBR034C HMT1 protein-arginine omega-N methyltransferase HMT1|RMT1|ODP1|HCP1 Nuclear SAM-dependent mono- and asymmetric methyltransferase; modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p, ribosomal protein Rps2p, and histones H3 and H4; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region peptidyl-amino acid modification, protein alkylation, nucleobase-containing compound transport, DNA-templated transcription, termination, nuclear transport, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation transferase activity, methyltransferase activity nucleus BY4742 [2-2] E 4 YBR035C PDX3 pyridoxamine-phosphate oxidase PDX3 Pyridoxine (pyridoxamine) phosphate oxidase; has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism cofactor metabolic process ion binding, oxidoreductase activity mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-2] E 5 YBR036C CSG2 mannosylinositol phosphorylceramide synthase regulatory subunit|CLS2 Endoplasmic reticulum membrane protein; required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress cellular ion homeostasis, lipid metabolic process enzyme regulator activity endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [2-2] E 6 YBR037C SCO1 Cu-binding protein SCO1|PET161 Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication ion transport, protein complex biogenesis oxidoreductase activity, ion binding mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-2] E 7 - mitochondrion, membrane, mitochondrial envelope, cytoplasm mitochondrion, membrane, mitochondrial envelope, cytoplasm mitochondrion, membrane, mitochondrial envelope, cytoplasm - - - - [2-2] E 8 YBR039W ATP3 F1F0 ATP synthase subunit gamma Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis transmembrane transport, ion transport, nucleobase-containing small molecule metabolic process hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-2] E 9 YBR040W FIG1 - Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating response to chemical, cell morphogenesis, conjugation molecular_function vacuole, cell wall, membrane, site of polarized growth, cytoplasm BY4742 [2-2] E 10 YBR041W FAT1 long-chain fatty acid transporter FAT1 Very long chain fatty acyl-CoA synthetase and fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids ion transport, monocarboxylic acid metabolic process, lipid transport, lipid metabolic process ligase activity peroxisome, endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [2-2] E 11 YBR042C CST26 putative acyltransferase|PSI1 Acyltransferase; enzyme mainly responsible for the introduction of saturated very long chain fatty acids into neo-synthesized molecules of phosphatidylinositol; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication lipid metabolic process transferase activity other BY4742 [2-2] E 12 YBR043C QDR3 AQR2 Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; involved in spore wall asembly; sequence similarity to DTR1 and QDR1, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin meiotic cell cycle, response to chemical, sporulation, transmembrane transport, cell wall organization or biogenesis transmembrane transporter activity membrane, plasma membrane BY4742 [2-2] F 1 YBR044C TCM62 - Protein involved in assembly of the succinate dehydrogenase complex; mitochondrial; putative chaperone mitochondrion organization, protein complex biogenesis unfolded protein binding membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-2] F 2 YBR045C GIP1 protein phosphatase regulator GIP1 Meiosis-specific regulatory subunit of the Glc7p protein phosphatase; regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p meiotic cell cycle, sporulation, protein dephosphorylation, regulation of protein modification process, cell wall organization or biogenesis enzyme regulator activity, enzyme binding membrane, cytoplasm BY4742 [2-2] F 3 YBR046C ZTA1 NADPH:quinone reductase NADPH-dependent quinone reductase; GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin response to chemical, response to oxidative stress RNA binding, oxidoreductase activity nucleus, cytoplasm BY4742 [2-2] F 4 YBR047W FMP23 - Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [2-2] F 5 YBR048W RPS11B uS17|ribosomal 40S subunit protein S11B|S17|rp41B|YS12|S18B|S11B Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [2-2] F 6 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [2-2] F 7 YBR050C REG2 protein phosphatase regulator REG2 Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease; REG2 has a paralog, REG1, that arose from the whole genome duplication transcription from RNA polymerase II promoter enzyme regulator activity cytoplasm BY4742 [2-2] F 8 YBR051W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase biological_process molecular_function cellular_component BY4742 [2-2] F 9 YBR052C RFS1 flavodoxin-like fold family protein Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication biological_process molecular_function membrane, cytoplasm BY4742 [2-2] F 10 YBR053C - - Putative protein of unknown function; induced by cell wall perturbation biological_process molecular_function cellular_component BY4742 [2-2] F 11 YBR054W YRO2 - Protein with a putative role in response to acid stress; null mutant is sensitive to acetic acid; transcription is regulated by Haa1p and induced in the presence of acetic acid; protein observed in plasma membrane foci in the presence of acetic acid; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies biological_process molecular_function cellular bud, membrane, endomembrane system, mitochondrion, endoplasmic reticulum, plasma membrane, cytoplasm BY4742 [2-2] F 12 - cellular bud, membrane, endomembrane system, mitochondrion, endoplasmic reticulum, plasma membrane, cytoplasm cellular bud, membrane, endomembrane system, mitochondrion, endoplasmic reticulum, plasma membrane, cytoplasm cellular bud, membrane, endomembrane system, mitochondrion, endoplasmic reticulum, plasma membrane, cytoplasm - - - - [2-2] G 1 YBR056W - 17-beta-hydroxysteroid dehydrogenase-like protein Putative glycoside hydrolase of the mitochondrial intermembrane space biological_process molecular_function mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-2] G 2 YBR057C MUM2 SPOT8 Protein essential for meiotic DNA replication and sporulation; cytoplasmic protein; subunit of the MIS complex which controls mRNA methylation during during the induction of sporulation; also interacts with Orc2p, which is a component of the origin recognition complex meiotic cell cycle, organelle fission, DNA replication, pseudohyphal growth, RNA modification other cytoplasm BY4742 [2-2] G 3 YBR058C UBP14 ubiquitin-specific protease UBP14|GID6 Ubiquitin-specific protease; specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process peptidase activity, hydrolase activity cytoplasm BY4742 [2-2] G 4 YBR059C AKL1 serine/threonine protein kinase AKL1 Ser-Thr protein kinase; member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization protein phosphorylation, cytoskeleton organization, regulation of transport, endocytosis kinase activity, transferase activity cellular bud, membrane, site of polarized growth, cytoplasm BY4742 [2-2] G 5 - cellular bud, membrane, site of polarized growth, cytoplasm cellular bud, membrane, site of polarized growth, cytoplasm cellular bud, membrane, site of polarized growth, cytoplasm - - - - [2-2] G 6 - - - - - - - - [2-2] G 7 YBR062C - - Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway biological_process molecular_function cellular_component BY4742 [2-2] G 8 YBR063C - - Putative protein of unknown function; YBR063C is not an essential gene biological_process molecular_function cellular_component BY4742 [2-2] G 9 YBR064W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR063C biological_process molecular_function cellular_component BY4742 [2-2] G 10 YBR065C ECM2 SLT11 Pre-mRNA splicing factor; facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p RNA splicing, mRNA processing molecular_function nucleus BY4742 [2-2] G 11 YBR066C NRG2 - Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; activated in stochastic pulses of nuclear localization in response to low glucose response to chemical, invasive growth in response to glucose limitation, pseudohyphal growth, transcription from RNA polymerase II promoter, response to osmotic stress DNA binding nucleus BY4742 [2-2] G 12 YBR067C TIP1 putative lipase Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins cell wall organization or biogenesis structural molecule activity, hydrolase activity cell wall, cytoplasm BY4742 [2-2] H 1 YBR068C BAP2 branched-chain amino acid permease BAP2 High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication transmembrane transport, ion transport, amino acid transport transmembrane transporter activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [2-2] H 2 YBR069C TAT1 amino acid transporter TAT1|VAP1|TAP1 Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress transmembrane transport, ion transport, amino acid transport transmembrane transporter activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [2-2] H 3 - endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane - - - - [2-2] H 4 YBR071W - - Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking biological_process molecular_function cellular bud, site of polarized growth, cytoplasm BY4742 [2-2] H 5 YBR072W HSP26 chaperone protein HSP26 Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic foci; oligomer activation requires heat-induced conformational change; also has mRNA binding activity response to heat, protein folding RNA binding, unfolded protein binding, mRNA binding nucleus, mitochondrion, cytoplasm BY4742 [2-2] H 6 YBR073W RDH54 DNA-dependent ATPase RDH54|TID1 DNA-dependent ATPase; DNA recombination/repair translocase, supercoils DNA and promotes DNA strand opening; stimulates strand exchange by modifying dsDNA topology; involved in recombinational repair of DNA double-strand breaks (DSBs) during mitosis and meiosis; phosphorylated in Mec1p-, Rad53p-dependent way in response to one DSB; contributes to remodelling of nucleosomes; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p meiotic cell cycle, DNA repair, organelle fission, cellular response to DNA damage stimulus, chromosome segregation, DNA recombination hydrolase activity, DNA binding, ATPase activity nucleus BY4742 [2-2] H 7 YBR074W PFF1 YBR075W Multi-spanning vacuolar membrane protease; glycosylated transmembrane protein bearing homology to the M28 family of metalloproteases; has a lumenal-facing protease domain; proposed role in vacuole physiology biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [2-2] H 8 YBR075W membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - BY4742 [2-2] H 9 YBR076W ECM8 - Non-essential protein of unknown function biological_process molecular_function cellular_component BY4742 [2-2] H 10 YBR077C SLM4 GSE1|NIR1|EGO3 Component of the EGO and GSE complexes; essential for integrity and function of EGO; EGO is involved in the regulation of microautophagy and GSE is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 signaling molecular_function membrane, cytoplasmic vesicle, endomembrane system, vacuole, cytoplasm BY4742 [2-2] H 11 YBR078W ECM33 - GPI-anchored protein of unknown function; possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p; ECM33 has a paralog, PST1, that arose from the whole genome duplication cell wall organization or biogenesis molecular_function cell wall, membrane, endomembrane system, mitochondrion, endoplasmic reticulum, plasma membrane, cytoplasm BY4742 [2-2] H 12 - cell wall, membrane, endomembrane system, mitochondrion, endoplasmic reticulum, plasma membrane, cytoplasm cell wall, membrane, endomembrane system, mitochondrion, endoplasmic reticulum, plasma membrane, cytoplasm cell wall, membrane, endomembrane system, mitochondrion, endoplasmic reticulum, plasma membrane, cytoplasm - - - - [2-3] A 1 - - - - - - - - [2-3] A 2 YBR081C SPT7 SAGA histone acetyltransferase complex subunit SPT7|GIT2 Subunit of the SAGA transcriptional regulatory complex; involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex peptidyl-amino acid modification, protein acylation, chromatin organization, protein complex biogenesis, histone modification, conjugation structural molecule activity nucleus, mitochondrion, cytoplasm BY4742 [2-3] A 3 - nucleus, mitochondrion, cytoplasm nucleus, mitochondrion, cytoplasm nucleus, mitochondrion, cytoplasm - - - - [2-3] A 4 - - - - - - - - [2-3] A 5 - - - - - - - - [2-3] A 6 YBR084W MIS1 trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase MIS1 Mitochondrial C1-tetrahydrofolate synthase; involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase cofactor metabolic process, vitamin metabolic process ligase activity, hydrolase activity, oxidoreductase activity mitochondrion, cytoplasm BY4742 [2-3] A 7 YBR085W AAC3 ADP/ATP carrier protein AAC3|ANC3 Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication transmembrane transport, generation of precursor metabolites and energy, cellular respiration transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-3] A 8 - membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm - - - - [2-3] A 9 - - - - - - - - [2-3] A 10 - - - - - - - - [2-3] A 11 - - - - - - - - [2-3] A 12 - - - - - - - - [2-3] B 1 YBR090C-A - - - - - - BY4742 [2-3] B 2 - - - - - - - - [2-3] B 3 YBR092C PHO3 acid phosphatase PHO3|phoC Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin vitamin metabolic process phosphatase activity, hydrolase activity endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-3] B 4 YBR093C PHO5 acid phosphatase PHO5|phoE Repressible acid phosphatase; 1 of 3 repressible acid phosphatases that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 response to starvation phosphatase activity, hydrolase activity cell wall BY4742 [2-3] B 5 YBR094W PBY1 putative tubulin tyrosine ligase Putative tubulin tyrosine ligase associated with P-bodies; may have a role in mRNA metabolism; yeast knockout collection strain identified as a pby1 null mutant is actually wild-type for PBY1 and deleted for mms4 biological_process molecular_function cytoplasm BY4742 [2-3] B 6 YBR095C RXT2 RAF60 Component of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth; relocalizes to the cytosol in response to hypoxia invasive growth in response to glucose limitation, chromatin organization, conjugation, transcription from RNA polymerase II promoter hydrolase activity nucleus, chromosome, cytoplasm BY4742 [2-3] B 7 YBR096W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-3] B 8 YBR097W VPS15 ubiquitin-binding serine/threonine protein kinase VPS15|VPT15|VPS40|VPL19|VAC4|GRD8 Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery vacuole organization, protein targeting, protein phosphorylation, organelle inheritance, endosomal transport, transcription from RNA polymerase II promoter, signaling, DNA-templated transcription, elongation kinase activity, transferase activity, ubiquitin-like protein binding nucleus, endomembrane system, vacuole, mitochondrion, membrane, cytoplasm BY4742 [2-3] B 9 YBR098W MMS4 YBR100W|SLX2 Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdc5p during mitotic cell cycle activates the function of Mms4p-Mus81p meiotic cell cycle, DNA repair, organelle fission, regulation of organelle organization, cellular response to DNA damage stimulus, chromosome segregation, DNA recombination, regulation of DNA metabolic process, regulation of cell cycle hydrolase activity, nuclease activity nucleus BY4742 [2-3] B 10 YBR099C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 biological_process molecular_function cellular_component BY4742 [2-3] B 11 YBR100W cellular_component cellular_component cellular_component - - - BY4742 [2-3] B 12 YBR101C FES1 - Hsp70 (Ssa1p) nucleotide exchange factor; required for the release of misfolded proteins from the Hsp70 system to the Ub-proteasome machinery for destruction; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress cytoplasmic translation, response to chemical, proteolysis involved in cellular protein catabolic process enzyme regulator activity ribosome, cytoplasm BY4742 [2-3] C 1 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [2-3] C 2 YBR103W SIF2 EMB1 WD40 repeat-containing subunit of Set3C histone deacetylase complex; complex represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus meiotic cell cycle, protein phosphorylation, organelle fission, chromatin organization, regulation of organelle organization, histone modification, regulation of protein modification process, signaling, regulation of cell cycle molecular_function nucleus, chromosome BY4742 [2-3] C 3 YBR104W YMC2 organic acid transporter Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication transmembrane transport transmembrane transporter activity membrane, mitochondrion, cytoplasm BY4742 [2-3] C 4 YBR105C VID24 glucose-induced degradation complex subunit VID24|GID4 GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles carbohydrate metabolic process, protein targeting, proteolysis involved in cellular protein catabolic process molecular_function membrane, cytoplasmic vesicle, cytoplasm BY4742 [2-3] C 5 YBR106W PHO88 SND3 Protein involved in SRP-independent targeting of substrates to the ER; component of an alternative ER targeting pathway that has partial functional redundancy with the GET pathway; preference for substrates with downstream transmembrane domains; interacts with Snd1p, Env10p/Snd2p, and Sec61p-translocon subunits; can compensate for loss of SRP; role in phosphate transport, interacting with pho88, and in the maturation of secretory proteins ion transport, protein maturation molecular_function endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm BY4742 [2-3] C 6 YBR107C IML3 MCM19 Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; forms a stable complex with Chl4p; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 mitotic cell cycle, meiotic cell cycle, organelle fission, sporulation, chromosome segregation molecular_function nucleus, chromosome BY4742 [2-3] C 7 YBR108W AIM3 - Protein that inhibits barbed-end actin filament elongation; interacts with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss cytoskeleton organization, regulation of organelle organization, protein complex biogenesis molecular_function membrane, cell cortex, cytoskeleton, cytoplasm BY4742 [2-3] C 8 - membrane, cell cortex, cytoskeleton, cytoplasm membrane, cell cortex, cytoskeleton, cytoplasm membrane, cell cortex, cytoskeleton, cytoplasm - - - - [2-3] C 9 - - - - - - - - [2-3] C 10 YBR111C YSA1 ADP-ribose diphosphatase|RMA2 Nudix hydrolase family member with ADP-ribose pyrophosphatase activity; shown to metabolize O-acetyl-ADP-ribose to AMP and acetylated ribose 5'-phosphate other hydrolase activity nucleus, mitochondrion, cytoplasm BY4742 [2-3] C 11 - nucleus, mitochondrion, cytoplasm nucleus, mitochondrion, cytoplasm nucleus, mitochondrion, cytoplasm - - - - [2-3] C 12 YBR113W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 biological_process molecular_function membrane BY4742 [2-3] D 1 YBR114W RAD16 DNA repair protein RAD16|PSO5 Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad7p) during NER; required for NER of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex DNA repair, cellular response to DNA damage stimulus, proteolysis involved in cellular protein catabolic process, protein modification by small protein conjugation or removal transferase activity, hydrolase activity, DNA binding, ATPase activity nucleus BY4742 [2-3] D 2 YBR115C LYS2 L-aminoadipate-semialdehyde dehydrogenase Alpha aminoadipate reductase; catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p cellular amino acid metabolic process oxidoreductase activity cytoplasm BY4742 [2-3] D 3 YBR116C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 biological_process molecular_function cellular_component BY4742 [2-3] D 4 YBR117C TKL2 transketolase TKL2 Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL2 has a paralog, TKL1, that arose from the whole genome duplication cofactor metabolic process, nucleobase-containing small molecule metabolic process transferase activity nucleus, cytoplasm BY4742 [2-3] D 5 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [2-3] D 6 YBR119W MUD1 U1A|U1-A U1 snRNP A protein; homolog of human U1-A; involved in nuclear mRNA splicing RNA splicing, mRNA processing RNA binding nucleus BY4742 [2-3] D 7 YBR120C CBP6 - Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other ribosome, mitochondrion, cytoplasm BY4742 [2-3] D 8 - ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm - - - - [2-3] D 9 - - - - - - - - [2-3] D 10 - - - - - - - - [2-3] D 11 - - - - - - - - [2-3] D 12 - - - - - - - - [2-3] E 1 YBR126C TPS1 alpha,alpha-trehalose-phosphate synthase (UDP-forming) TPS1|TSS1|GLC6|GGS1|FDP1|CIF1|BYP1 Synthase subunit of trehalose-6-P synthase/phosphatase complex; synthesizes the storage carbohydrate trehalose, which is critically important for survival of long-term desiccation; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid response to heat, carbohydrate metabolic process, response to chemical, response to oxidative stress, oligosaccharide metabolic process transferase activity, transferring glycosyl groups, transferase activity cytoplasm BY4742 [2-3] E 2 YBR127C VMA2 H(+)-transporting V1 sector ATPase subunit B|ATPSV|VAT2 Subunit B of V1 peripheral membrane domain of vacuolar H+-ATPase; electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm; protein abundance increases in response to DNA replication stress; human homolog ATP6V1B1, implicated in autosomal-recessive distal renal tubular acidosis (RTA) with sensorineural deafness, complements yeast null mutant transmembrane transport, protein complex biogenesis, cellular ion homeostasis hydrolase activity, transmembrane transporter activity, ATPase activity vacuole, membrane, cytoplasm BY4742 [2-3] E 3 YBR128C ATG14 APG14|CVT12 Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I; Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor; other members are Vps34, Vps15, and Vps30p protein targeting molecular_function membrane, vacuole, cytoplasm BY4742 [2-3] E 4 YBR129C OPY1 - Protein of unknown function; overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [2-3] E 5 YBR130C SHE3 - Protein adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance organelle inheritance RNA binding, mRNA binding cellular bud, site of polarized growth, cytoplasm BY4742 [2-3] E 6 - cellular bud, site of polarized growth, cytoplasm cellular bud, site of polarized growth, cytoplasm cellular bud, site of polarized growth, cytoplasm - - - - [2-3] E 7 YBR132C AGP2 - Plasma membrane regulator of polyamine and carnitine transport; has similarity to transporters but lacks transport activity; may act as a sensor that transduces environmental signals; has a positive or negative regulatory effect on transcription of many transporter genes transmembrane transport, ion transport, regulation of transport molecular_function endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [2-3] E 8 YBR133C HSL7 protein arginine N-methyltransferase Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent localization to the bud neck in budded cells and periodic Hsl1p-dependent phosphorylation; required with Hsl1p, and Elm1p for the mother-bud neck recruitment, phosphorylation, and degradation of Swe1p; interacts directly with Swe1p; relocalizes away from bud neck upon DNA replication stress; human homolog PRMT5 can complement yeast hsl7 mutant mitotic cell cycle, regulation of cell cycle transferase activity, methyltransferase activity cell cortex, cytoskeleton, microtubule organizing center, cellular bud, site of polarized growth, cytoplasm BY4742 [2-3] E 9 YBR134W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [2-3] E 10 - cellular_component cellular_component cellular_component - - - - [2-3] E 11 - - - - - - - - [2-3] E 12 YBR137W - - Protein with a role in ER delivery of tail-anchored membrane proteins; interacts with Sgt2p; binds to the TRC complex, which inserts proteins into the ER membrane; interacts with Msn5p karyopherin; YBR137W is not an essential gene protein targeting molecular_function cytoplasm BY4742 [2-3] F 1 YBR138C - HDR1 Cytoplasmic protein of unknown function; APCC(Cdh1) substrate; potentially phosphorylated by Cdc28p; YBR138C is not an essential gene biological_process molecular_function cytoplasm BY4742 [2-3] F 2 YBR139W - carboxypeptidase C Putative serine type carboxypeptidase; role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner other peptidase activity, hydrolase activity vacuole, cytoplasm BY4742 [2-3] F 3 - vacuole, cytoplasm vacuole, cytoplasm vacuole, cytoplasm - - - - [2-3] F 4 YBR141C BMT2 25S rRNA (adenine2142-N1)-methyltransferase Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 2142; belongs to Rossmann fold superfamily; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene biological_process transferase activity, methyltransferase activity nucleus, nucleolus BY4742 [2-3] F 5 - nucleus, nucleolus nucleus, nucleolus nucleus, nucleolus - - - - [2-3] F 6 - - - - - - - - [2-3] F 7 YBR144C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene biological_process molecular_function cellular_component BY4742 [2-3] F 8 YBR145W ADH5 alcohol dehydrogenase ADH5 Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication carbohydrate metabolic process, cofactor metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, cellular amino acid metabolic process, nucleobase-containing small molecule metabolic process oxidoreductase activity nucleus, cytoplasm BY4742 [2-3] F 9 YBR146W MRPS9 mitochondrial 37S ribosomal protein MRPS9 Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [2-3] F 10 YBR147W RTC2 cationic amino acid transporter|YPQ3|RRT11 Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter; RTC2 has a paralog, YPQ1, that arose from the whole genome duplication transmembrane transport, ion transport, amino acid transport transmembrane transporter activity vacuole, mitochondrion, membrane, cytoplasm BY4742 [2-3] F 11 YBR148W YSW1 - Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane; YSW1 has a paralog, SPO21, that arose from the whole genome duplication meiotic cell cycle, sporulation molecular_function membrane, cell cortex, cytoskeleton, cytoplasm BY4742 [2-3] F 12 YBR149W ARA1 D-arabinose 1-dehydrogenase (NAD(P)(+)) ARA1 NADP+ dependent arabinose dehydrogenase; involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product carbohydrate metabolic process oxidoreductase activity cytoplasm BY4742 [2-3] G 1 - cytoplasm cytoplasm cytoplasm - - - - [2-3] G 2 YBR151W APD1 - Protein of unknown function; required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus biological_process molecular_function nucleus, cytoplasm BY4742 [2-3] G 3 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [2-3] G 4 - - - - - - - - [2-3] G 5 - - - - - - - - [2-3] G 6 - - - - - - - - [2-3] G 7 YBR156C SLI15 - Subunit of the conserved chromosomal passenger complex (CPC); complex regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; other complex members are Ipl1p, Bir1p, and Nbl1p protein phosphorylation, cytokinesis, regulation of cell cycle, chromosome segregation enzyme regulator activity cytoskeleton BY4742 [2-3] G 8 YBR157C ICS2 - Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization biological_process molecular_function cellular_component BY4742 [2-3] G 9 YBR158W AMN1 CST13|ICS4 Protein required for daughter cell separation; multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) mitotic cell cycle, organelle fission, regulation of organelle organization, regulation of cell cycle other nucleus, cellular bud, cytoplasm BY4742 [2-3] G 10 YBR159W IFA38 ketoreductase Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides lipid metabolic process, monocarboxylic acid metabolic process oxidoreductase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-3] G 11 - membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm - - - - [2-3] G 12 YBR161W CSH1 mannosylinositol phosphorylceramide synthase catalytic subunit CSH1 Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication lipid metabolic process transferase activity, transferring glycosyl groups, transferase activity membrane, vacuole, cytoplasm BY4742 [2-3] H 1 YBR162C TOS1 - Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C biological_process molecular_function cell wall, vacuole, cytoplasm BY4742 [2-3] H 2 YBR162W-A YSY6 - Protein of unknown function; expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion other molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-3] H 3 YBR163W EXO5 DEM1 Mitochondrial 5'-3' exonuclease and sliding exonuclease; required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 mitochondrion organization nuclease activity, hydrolase activity mitochondrion, cytoplasm BY4742 [2-3] H 4 YBR164C ARL1 Arf family GTPase ARL1|DLP2 Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; role in membrane organization at trans-Golgi network; required for delivery of Atg9p to the phagophore assembly site during autophagy under high temperature stress; required with Ypt6p for starvation-induced autophagy; required for the CVT pathway under non-starvation conditions; G protein of the Ras superfamily, similar to ADP-ribosylation factor vacuole organization, protein targeting, organelle assembly, Golgi vesicle transport, endocytosis GTPase activity, hydrolase activity endomembrane system, Golgi apparatus, cytoplasm BY4742 [2-3] H 5 YBR165W UBS1 - Ubiquitin-conjugating enzyme suppressor that regulates Cdc34p; functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity protein modification by small protein conjugation or removal, nuclear transport molecular_function nucleus BY4742 [2-3] H 6 YBR166C TYR1 prephenate dehydrogenase (NADP(+)) Prephenate dehydrogenase involved in tyrosine biosynthesis; expression is dependent on phenylalanine levels cellular amino acid metabolic process oxidoreductase activity cytoplasm BY4742 [2-3] H 7 - cytoplasm cytoplasm cytoplasm - - - - [2-3] H 8 - - - - - - - - [2-3] H 9 - - - - - - - - [2-3] H 10 YBR170C NPL4 HRD4 Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) mitotic cell cycle, response to chemical, organelle fission, cytoskeleton organization, proteolysis involved in cellular protein catabolic process, RNA catabolic process, regulation of DNA metabolic process molecular_function nucleus, endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm BY4742 [2-3] H 11 YBR171W SEC66 KAR7|Sec63 complex subunit SEC66|SEC71|HSS1 Non-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec63p, Sec62p, and Sec72p protein targeting, transmembrane transport protein transporter activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-3] H 12 YBR172C SMY2 - GYF domain protein; involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication Golgi vesicle transport molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-4] A 1 YBR173C UMP1 RNS2 Chaperone required for correct maturation of the 20S proteasome; short-lived chaperone; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly cellular response to DNA damage stimulus, proteolysis involved in cellular protein catabolic process, protein complex biogenesis molecular_function nucleus, cytoplasm BY4742 [2-4] A 2 YBR174C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective biological_process molecular_function cellular_component BY4742 [2-4] A 3 YBR175W SWD3 SAF35|CPS30 Essential subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 peptidyl-amino acid modification, meiotic cell cycle, telomere organization, protein alkylation, organelle fission, chromatin organization, regulation of organelle organization, histone modification, regulation of cell cycle transferase activity, methyltransferase activity nucleus BY4742 [2-4] A 4 YBR176W ECM31 3-methyl-2-oxobutanoate hydroxymethyltransferase Ketopantoate hydroxymethyltransferase; required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate cofactor metabolic process, vitamin metabolic process, monocarboxylic acid metabolic process transferase activity mitochondrion, cytoplasm BY4742 [2-4] A 5 YBR177C EHT1 medium-chain fatty acid ethyl ester synthase/esterase Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication monocarboxylic acid metabolic process, lipid metabolic process transferase activity, hydrolase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-4] A 6 YBR178W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C biological_process molecular_function cellular_component BY4742 [2-4] A 7 YBR179C FZO1 mitofusin Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system mitochondrion organization, organelle fusion, membrane fusion GTPase activity, hydrolase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-4] A 8 YBR180W DTR1 - Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; sequence similarity to QDR1 and QDR3, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expressed during sporulation meiotic cell cycle, sporulation, transmembrane transport, cell wall organization or biogenesis transmembrane transporter activity membrane BY4742 [2-4] A 9 YBR181C RPS6B eS6|ribosomal 40S subunit protein S6B|S6e|RPS102|RPS101|LPG18 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [2-4] A 10 YBR182C SMP1 - MADS-box transcription factor involved in osmotic stress response; SMP1 has a paralog, RLM1, that arose from the whole genome duplication transcription from RNA polymerase II promoter, response to osmotic stress nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [2-4] A 11 YBR183W YPC1 phytoceramidase Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication lipid metabolic process transferase activity, hydrolase activity, hydrolase activity, acting on glycosyl bonds cell cortex, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [2-4] A 12 YBR184W - - Putative protein of unknown function; YBR184W is not an essential gene biological_process molecular_function cellular_component BY4742 [2-4] B 1 YBR185C MBA1 - Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-4] B 2 YBR186W PCH2 - Hexameric ring ATPase that remodels chromosome axis protein Hop1p; nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in rDNA; required for meiotic double-stranded break formation meiotic cell cycle, organelle fission, regulation of organelle organization, DNA recombination, regulation of cell cycle hydrolase activity, ATPase activity nucleus, nucleolus BY4742 [2-4] B 3 YBR187W GDT1 putative ribosome biosynthesis protein GDT1 Calcium transporter localized to the cis- and medial-Golgi apparatus; required for protein glycosylation; GFP-fusion protein localizes to the vacuole; TMEM165, a human gene which causes Congenital Disorders of Glycosylation is orthologous and functionally complements the null allele; expression pattern and physical interactions suggest a possible role in ribosome biogenesis; expression reduced in a gcr1 null mutant transmembrane transport, ion transport, cellular ion homeostasis transmembrane transporter activity endomembrane system, vacuole, membrane, Golgi apparatus, cytoplasm BY4742 [2-4] B 4 YBR188C NTC20 - Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs RNA splicing, mRNA processing other nucleus BY4742 [2-4] B 5 - nucleus nucleus nucleus - - - - [2-4] B 6 - - - - - - - - [2-4] B 7 - - - - - - - - [2-4] B 8 - - - - - - - - [2-4] B 9 - - - - - - - - [2-4] B 10 YBR194W AIM4 SOY1 Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress biological_process molecular_function cytoplasm BY4742 [2-4] B 11 YBR195C MSI1 CAC3 Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 affects multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of DNA damage checkpoint after DNA repair; chromatin dynamics during transcription; and repression of divergent noncoding transcription; Msi1p localizes to nucleus and cytoplasm and independently regulates the RAS/cAMP pathway via sequestration of Npr1p kinase chromatin organization histone binding nucleus, cytoplasm BY4742 [2-4] B 12 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [2-4] C 1 YBR197C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication biological_process molecular_function nucleus, cytoplasm BY4742 [2-4] C 2 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [2-4] C 3 YBR199W KTR4 putative mannosyltransferase Glycosyltransferase involved in protein glycosylation; transfers GDP-mannose to methyl-alpha-mannoside in vitro; member of the KRE2/MNT1 mannosyltransferase family of type II membrane proteins with a short cytoplasmic N-terminus, a membrane-spanning region and a highly conserved catalytic lumenal domain protein glycosylation transferase activity, transferring glycosyl groups, transferase activity endomembrane system, vacuole, cytoplasm, Golgi apparatus BY4742 [2-4] C 4 YBR200W BEM1 phosphatidylinositol-3-phosphate-binding protein BEM1|SRO1 Protein containing SH3-domains; involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p response to chemical, cell morphogenesis, conjugation lipid binding, structural molecule activity, ion binding mitochondrion, cellular bud, site of polarized growth, cytoplasm BY4742 [2-4] C 5 YBR201W DER1 derlin ER membrane protein that promotes export of misfolded polypeptides; required for ER-associated protein degradation of misfolded or unassembled proteins; initiates export of aberrant polypeptides from ER lumen by threading them into ER membrane and routing them to Hrd1p for ubiquitination; function normally requires N-terminal acetylation by NatB; N- and C- termini protrude into cytoplasm; similar to Dfm1p; homolog of mammalian derlin-1 response to chemical, proteolysis involved in cellular protein catabolic process other membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-4] C 6 - membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm - - - - [2-4] C 7 YBR203W COS111 - Protein required for antifungal drug ciclopirox olamine resistance; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies signaling molecular_function mitochondrion, cytoplasm BY4742 [2-4] C 8 YBR204C LDH1 triacylglycerol lipase Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) other hydrolase activity endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [2-4] C 9 YBR205W KTR3 mannosyltransferase KTR3 Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress protein glycosylation transferase activity, transferring glycosyl groups, transferase activity nucleus, membrane, vacuole, cytoplasm BY4742 [2-4] C 10 YBR206W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 biological_process molecular_function cellular_component BY4742 [2-4] C 11 YBR207W FTH1 - Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress transmembrane transport, ion transport, endocytosis transmembrane transporter activity endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [2-4] C 12 YBR208C DUR1,2 bifunctional urea carboxylase/allophanate hydrolase|DUR80 Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress other ligase activity, hydrolase activity cytoplasm BY4742 [2-4] D 1 YBR209W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YBR209W is not an essential gene biological_process molecular_function cellular_component BY4742 [2-4] D 2 YBR210W ERV15 - Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication mitotic cell cycle, cell budding, cytokinesis, Golgi vesicle transport molecular_function cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [2-4] D 3 - cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, cytoplasm cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, cytoplasm cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, cytoplasm - - - - [2-4] D 4 YBR212W NGR1 RBP1 RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase regulation of translation, mitochondrion organization RNA binding, mRNA binding cytoplasm BY4742 [2-4] D 5 YBR213W MET8 bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase MET8 Bifunctional dehydrogenase and ferrochelatase; involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis cofactor metabolic process lyase activity, oxidoreductase activity cellular_component BY4742 [2-4] D 6 YBR214W SDS24 - Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication endocytosis molecular_function cytoplasm BY4742 [2-4] D 7 YBR215W HPC2 - Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes mitotic cell cycle, chromatin organization, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation chromatin binding, DNA binding nucleus BY4742 [2-4] D 8 YBR216C YBP1 - Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication response to chemical, response to oxidative stress molecular_function cytoplasm BY4742 [2-4] D 9 YBR217W ATG12 APG12 Ubiquitin-like modifier involved in autophagy and the Cvt pathway; conserved; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg5p-Atg12p conjugate enhances E2 activity of Atg3p by rearranging its catalytic site, also forms a complex with Atg16p; the Atg5-Atg12/Atg16 complex binds to membranes and is essential for autophagosome formation vacuole organization, protein targeting, mitochondrion organization, organelle assembly, protein lipidation transferase activity, enzyme regulator activity cytoplasm BY4742 [2-4] D 10 YBR218C PYC2 pyruvate carboxylase 2 Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication carbohydrate metabolic process ligase activity cytoplasm BY4742 [2-4] D 11 YBR219C - - Putative protein of unknown function; YBR219C is not an essential gene biological_process molecular_function membrane BY4742 [2-4] D 12 YBR220C - - Putative protein of unknown function; YBR220C is not an essential gene biological_process molecular_function membrane BY4742 [2-4] E 1 YBR221C PDB1 pyruvate dehydrogenase (acetyl-transferring) subunit E1 beta E1 beta subunit of the pyruvate dehydrogenase (PDH) complex; PDH is an evolutionarily conserved multi-protein complex found in mitochondria cofactor metabolic process, monocarboxylic acid metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4742 [2-4] E 2 YBR222C PCS60 FAT2 Oxalyl-CoA synthetase; capable of catalyzing conversion of oxalate to oxalyl-CoA; catalyzes first step in pathway of oxalate degradation that functions to protect yeast from inhibitory effects of oxalate; peroxisomal protein that binds mRNA; localizes to both peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid; similar to E. coli long chain acyl-CoA synthetase other RNA binding, mRNA binding, ligase activity peroxisome, membrane, cytoplasm BY4742 [2-4] E 3 YBR223C TDP1 tyrosyl-DNA phosphodiesterase 1 Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topo I and topo II; mutations in the human homolog, TDP1, result in the a neurodegenerative disease, spinocerebellar ataxia with axonal neuropathy (SCAN1); yeast cells and human rhabdomyosarcoma lines that overexpress TDP1 both exhibit elevated dosage chromosomal instability (dCIN) DNA repair, cellular response to DNA damage stimulus hydrolase activity nucleus BY4742 [2-4] E 4 YBR224W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 biological_process molecular_function membrane BY4742 [2-4] E 5 YBR225W - - Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components biological_process molecular_function cellular_component BY4742 [2-4] E 6 YBR226C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W biological_process molecular_function cellular_component BY4742 [2-4] E 7 YBR227C MCX1 - Non-proteolytic ATPase of the AAA family; stimulates incorporation of the pyridoxal phosphate cofactor into Hem1p (5-aminolevulinic acid synthase); localized to the mitochondrial matrix; ortholog of vertebrate CLPX, which promotes erythropoiesis protein targeting, mitochondrion organization, transmembrane transport, regulation of protein modification process, protein folding hydrolase activity, ion binding, ATPase activity mitochondrion, cytoplasm BY4742 [2-4] E 8 YBR228W SLX1 endonuclease Endonuclease involved in DNA recombination and repair; subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p DNA replication nuclease activity, hydrolase activity nucleus, chromosome BY4742 [2-4] E 9 YBR229C ROT2 glucan 1,3-alpha-glucosidase ROT2|GLS2 Glucosidase II catalytic subunit; required to trim the final glucose in N-linked glycans; required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations carbohydrate metabolic process, protein glycosylation, cell wall organization or biogenesis hydrolase activity, hydrolase activity, acting on glycosyl bonds endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [2-4] E 10 YBR230C OM14 - Mitochondrial outer membrane receptor for cytosolic ribosomes; integral protein of the outer membrane that interacts with the nascent chain-associated complex (NAC) bound to ribosomes, contributing to co-translational mitochondrial import; interacts with porin (Por1p) and Om45p; abundance is decreased in cells grown in glucose relative to other carbon sources protein targeting, mitochondrion organization other membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-4] E 11 YBR231C SWC5 AOR1 Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia chromatin organization molecular_function nucleus, chromosome, cytoplasm BY4742 [2-4] E 12 - nucleus, chromosome, cytoplasm nucleus, chromosome, cytoplasm nucleus, chromosome, cytoplasm - - - - [2-4] F 1 YBR233W PBP2 HEK1 RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress telomere organization RNA binding, mRNA binding nucleus, cytoplasm BY4742 [2-4] F 2 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [2-4] F 3 YBR235W VHC1 - Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs); similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family transmembrane transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [2-4] F 4 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [2-4] F 5 - - - - - - - - [2-4] F 6 YBR238C - - Mitochondrial membrane protein; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span; YBR238C has a paralog, RMD9, that arose from the whole genome duplication generation of precursor metabolites and energy, cellular respiration RNA binding, mRNA binding mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-4] F 7 YBR239C ERT1 - Transcriptional regulator; involved in regulation of gluconeogenesis and fermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition; member of the zinc cluster family of proteins, similar to Rds2p carbohydrate metabolic process, response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [2-4] F 8 YBR240C THI2 phoF|PHO6 Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type vitamin metabolic process, transcription from RNA polymerase II promoter DNA binding nucleus BY4742 [2-4] F 9 YBR241C - - Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene; YBR241C has a paralog, VPS73, that arose from the whole genome duplication transmembrane transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [2-4] F 10 YBR242W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene; YBR242W has a paralog, YGL101W, that arose from the whole genome duplication biological_process molecular_function nucleus, cytoplasm BY4742 [2-4] F 11 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [2-4] F 12 YBR244W GPX2 glutathione peroxidase GPX2|AMI1 Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress response to chemical, response to oxidative stress oxidoreductase activity nucleus, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [2-4] G 1 YBR245C ISW1 chromatin-remodeling ATPase ISW1|SGN2 ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; with Ioc3p forms Isw1a complex involved in repression of transcription initiation; with Ioc2p and Ioc4p forms Isw1b complex involved in regulation of transcription elongation; Isw1b recruited to ORFs by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions; Isw1p import into nucleus depends on C-terminal bipartite nuclear targeting signal KRIR X19 KKAK transcription from RNA polymerase I promoter, chromatin organization, protein complex biogenesis, chromosome segregation, DNA-templated transcription, termination, DNA-templated transcription, initiation, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation chromatin binding, hydrolase activity, DNA binding, ATPase activity nucleus, chromosome, nucleolus BY4742 [2-4] G 2 YBR246W RRT2 DPH7|diphthamide synthase|ERE1 Methylesterase performing penultimate step of diphthamide biosynthesis; hydrolyzes methylated diphthine to produce diphthine and allows Dph6-catalyzed amidation reaction to occur; deletion leads to resistance to sordarin and accumulation of methylatediphthine; WD40 domain-containing protein; involved in endosomal recycling; forms complex with Rtt10p that functions in retromer-mediated pathway for recycling internalized cell-surface proteins peptidyl-amino acid modification, endosomal transport hydrolase activity cytoplasmic vesicle, endomembrane system, cytoplasm BY4742 [2-4] G 3 - cytoplasmic vesicle, endomembrane system, cytoplasm cytoplasmic vesicle, endomembrane system, cytoplasm cytoplasmic vesicle, endomembrane system, cytoplasm - - - - [2-4] G 4 YBR248C HIS7 imidazoleglycerol-phosphate synthase Imidazole glycerol phosphate synthase; glutamine amidotransferase:cyclase that catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor cellular amino acid metabolic process transferase activity, transferring glycosyl groups, transferase activity, hydrolase activity other BY4742 [2-4] G 5 YBR249C ARO4 3-deoxy-7-phosphoheptulonate synthase ARO4 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress other transferase activity nucleus, cytoplasm BY4742 [2-4] G 6 YBR250W SPO23 - Protein of unknown function; associates with meiosis-specific protein Spo1p meiotic cell cycle, organelle fission molecular_function cellular_component BY4742 [2-4] G 7 YBR251W MRPS5 mitochondrial 37S ribosomal protein MRPS5 Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [2-4] G 8 - ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm - - - - [2-4] G 9 - - - - - - - - [2-4] G 10 - - - - - - - - [2-4] G 11 YBR255W MTC4 - Protein of unknown function; required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 biological_process molecular_function cytoplasm BY4742 [2-4] G 12 - cytoplasm cytoplasm cytoplasm - - - - [2-4] H 1 - - - - - - - - [2-4] H 2 YBR258C SHG1 CPS15 Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres peptidyl-amino acid modification, protein alkylation, chromatin organization, histone modification transferase activity, methyltransferase activity nucleus BY4742 [2-4] H 3 YBR259W - - Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress biological_process molecular_function cellular_component BY4742 [2-4] H 4 YBR260C RGD1 - GTPase-activating protein (RhoGAP) for Rho3p and Rho4p; possibly involved in control of actin cytoskeleton organization signaling, response to osmotic stress lipid binding, enzyme regulator activity, ion binding cell cortex, cytoskeleton, cellular bud, membrane, site of polarized growth, cytoplasm BY4742 [2-4] H 5 YBR261C TAE1 N-terminal protein methyltransferase|NTM1 AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm peptidyl-amino acid modification, cytoplasmic translation, protein alkylation transferase activity, methyltransferase activity cytoplasm BY4742 [2-4] H 6 YBR262C MIC12 AIM5|MCS12|FMP51 Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; forms a subcomplex with Mic10p and Mic27p whose assembly and stability requires cardiolipin mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [2-4] H 7 YBR263W SHM1 glycine hydroxymethyltransferase SHM1|TMP3|SHMT1 Mitochondrial serine hydroxymethyltransferase; converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine cellular amino acid metabolic process transferase activity mitochondrion, cytoplasm BY4742 [2-4] H 8 YBR264C YPT10 Rab family GTPase YPT10 Rab family GTP-binding protein; contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles other GTPase activity, hydrolase activity membrane, vacuole, cytoplasm BY4742 [2-4] H 9 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [2-4] H 10 YBR266C SLM6 - Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase endocytosis molecular_function cellular_component BY4742 [2-4] H 11 YBR267W REI1 - Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network mitotic cell cycle, cell budding, ribosomal large subunit biogenesis, nuclear transport DNA binding ribosome, cytoplasm BY4742 [2-4] H 12 YBR268W MRPL37 mitochondrial 54S ribosomal protein YmL37|YmL37 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [3-1] A 1 YCL001W RER1 protein retrieval receptor Protein involved in retention of membrane proteins; including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Golgi vesicle transport molecular_function membrane, cytoplasmic vesicle, endomembrane system, vacuole, cytoplasm, Golgi apparatus BY4742 [3-1] A 2 YCL002C - - Putative protein of unknown function; YCL002C is not an essential gene biological_process molecular_function nucleus, membrane, cytoplasm BY4742 [3-1] A 3 YCL003W nucleus, membrane, cytoplasm nucleus, membrane, cytoplasm nucleus, membrane, cytoplasm - - - BY4742 [3-1] A 4 - - - - - - - - [3-1] A 5 YCL005W LDB16 - Protein involved in lipid droplet (LD) assembly; forms a complex with Sei1p at ER-LD contact sites, stabilizing contact sites; ensures that LDs bud from the ER towards the cytosolic side of the membrane; null mutants have decreased net negative cell surface charge and localized accumulation of phosphatidic acid (PA) marker proteins; GFP-fusion protein expression is induced in response to MMS; null mutant can be complemented by the human seipin, BSCL2 other molecular_function membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [3-1] A 6 YCL006C membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm - - - BY4742 [3-1] A 7 YCL007C - CWH36 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps verified ORF YCL005W-A; mutations in YCL007C were thought to confer sensitivity to calcofluor white, but this phenotype was later shown to be due to the defect in YCL005W-A biological_process molecular_function cellular_component BY4742 [3-1] A 8 YCL008C STP22 ubiquitin-binding ESCRT-I subunit protein STP22|AGS1|VPL15|VPS23 Component of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; prevents polyubiquitination of the arrestin-related protein Rim8p, thereby directing its monoubiquitination by Rsp5p; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype; protein targeting, ion transport, proteolysis involved in cellular protein catabolic process, nucleobase-containing compound transport, regulation of protein modification process, protein modification by small protein conjugation or removal, endosomal transport ubiquitin-like protein binding cytoplasmic vesicle, endomembrane system, membrane, cytoplasm, plasma membrane BY4742 [3-1] A 9 YCL009C ILV6 acetolactate synthase regulatory subunit Regulatory subunit of acetolactate synthase; acetolactate synthase catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria cellular amino acid metabolic process transferase activity, enzyme regulator activity mitochondrion, cytoplasm BY4742 [3-1] A 10 YCL010C SGF29 - Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation peptidyl-amino acid modification, protein acylation, chromatin organization, transcription from RNA polymerase II promoter, histone modification histone binding, transcription factor activity, protein binding nucleus BY4742 [3-1] A 11 YCL011C GBP2 single-stranded telomeric DNA-binding/mRNA-binding protein|RLF6 Poly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; relocalizes to the cytosol in response to hypoxia; GBP2 has a paralog, HRB1, that arose from the whole genome duplication telomere organization, nucleobase-containing compound transport, RNA catabolic process, nuclear transport RNA binding, DNA binding, mRNA binding nucleus, cytoplasm BY4742 [3-1] A 12 YCL012W nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - BY4742 [3-1] B 1 YCL013W - - - - - - BY4742 [3-1] B 2 YCL014W BUD3 YCL012W Guanine nucleotide exchange factor (GEF) for Cdc42p; activates Cdc42p in early G1, accounting for the first stage of biphasic activation, with Cdc24p accounting for the second stage in late G1; involved in the Cdc42p-mediated assembly of the axial landmark that dictates the site for the next round of budding, resulting in the axial budding pattern observed in haploids; localizes with septins to the bud neck contractile ring in mitosis mitotic cell cycle, cell budding, cytokinesis, conjugation guanyl-nucleotide exchange factor activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [3-1] B 3 YCL016C DCC1 - Subunit of a complex with Ctf8p and Ctf18p; shares some components with Replication Factor C; required for sister chromatid cohesion and telomere length maintenance mitotic cell cycle, organelle fission, cellular response to DNA damage stimulus, chromosome segregation molecular_function chromosome BY4742 [3-1] B 4 - chromosome chromosome chromosome - - - - [3-1] B 5 - - - - - - - - [3-1] B 6 - - - - - - - - [3-1] B 7 - - - - - - - - [3-1] B 8 - - - - - - - - [3-1] B 9 - - - - - - - - [3-1] B 10 YCL023C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 biological_process molecular_function cellular_component BY4742 [3-1] B 11 YCL024W KCC4 serine/threonine protein kinase Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication mitotic cell cycle, cytoskeleton organization, cell budding, protein phosphorylation, organelle assembly, protein complex biogenesis, regulation of cell cycle ion binding, kinase activity, transferase activity, lipid binding cell cortex, cytoskeleton, plasma membrane, cellular bud, membrane, site of polarized growth, cytoplasm BY4742 [3-1] B 12 YCL025C AGP1 amino acid transporter AGP1|YCC5 Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication transmembrane transport, ion transport, amino acid transport transmembrane transporter activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [3-1] C 1 YCL026C endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane - - - BY4742 [3-1] C 2 YCL027W FUS1 - Membrane protein localized to the shmoo tip; required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate response to chemical, cell morphogenesis, conjugation molecular_function endomembrane system, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [3-1] C 3 YCL028W RNQ1 prion domain-containing protein RNQ1|[PIN(+)] [PIN(+)] prion; an infectious protein conformation that is generally an ordered protein aggregate biological_process molecular_function cytoplasm BY4742 [3-1] C 4 YCL029C BIK1 PAC14|ARM5 Microtubule-associated protein; component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 mitotic cell cycle, organelle fission, cytoskeleton organization, organelle fusion, conjugation, regulation of organelle organization, chromosome segregation, protein complex biogenesis, nucleus organization cytoskeletal protein binding nucleus, cell cortex, cytoskeleton, chromosome, microtubule organizing center, site of polarized growth, cytoplasm BY4742 [3-1] C 5 YCL030C HIS4 trifunctional histidinol dehydrogenase/phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase; phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis cellular amino acid metabolic process hydrolase activity, oxidoreductase activity cytoplasm BY4742 [3-1] C 6 - cytoplasm cytoplasm cytoplasm - - - - [3-1] C 7 YCL032W STE50 - Adaptor protein for various signaling pathways; involved in mating response, invasive/filamentous growth, osmotolerance; acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction response to heat, response to chemical, protein phosphorylation, conjugation, response to osmotic stress, signaling enzyme regulator activity cytoplasm BY4742 [3-1] C 8 YCL033C MXR2 MSRB Methionine-R-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan response to chemical, response to oxidative stress oxidoreductase activity mitochondrion, cytoplasm BY4742 [3-1] C 9 YCL034W LSB5 - Protein involved in membrane-trafficking events at plasma membrane; interacts with actin regulators Sla1p and Las17p, ubiquitin, Arf3p to couple actin dynamics to membrane trafficking processes; similar structure to GGA family of proteins with N-terminal VHS domain and GAT domain; binds Las17p, which is homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly, actin polymerization; may mediate disassembly of Pan1 complex from endocytic coat cytoskeleton organization, endocytosis molecular_function cell cortex, cytoskeleton, cytoplasm BY4742 [3-1] C 10 - cell cortex, cytoskeleton, cytoplasm cell cortex, cytoskeleton, cytoplasm cell cortex, cytoskeleton, cytoplasm - - - - [3-1] C 11 YCL036W GFD2 YCD6 Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4742 [3-1] C 12 YCL037C SRO9 - Cytoplasmic RNA-binding protein; shuttles between nucleus and cytoplasm and is exported from the nucleus in an mRNA export-dependent manner; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication cytoplasmic translation RNA binding, mRNA binding cytoplasm BY4742 [3-1] D 1 YCL038C ATG22 AUT4 Vacuolar integral membrane protein required for efflux of amino acids; required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation transmembrane transport, ion transport, amino acid transport molecular_function membrane, vacuole, cytoplasm BY4742 [3-1] D 2 YCL039W GID7 glucose-induced degradation complex subunit GID7|MOH2 Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions carbohydrate metabolic process, proteolysis involved in cellular protein catabolic process molecular_function nucleus, cytoplasm BY4742 [3-1] D 3 YCL040W GLK1 glucokinase|HOR3 Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication carbohydrate metabolic process, cofactor metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process, carbohydrate transport, nucleobase-containing small molecule metabolic process kinase activity, transferase activity membrane, cytoplasm, plasma membrane BY4742 [3-1] D 4 - membrane, cytoplasm, plasma membrane membrane, cytoplasm, plasma membrane membrane, cytoplasm, plasma membrane - - - - [3-1] D 5 YCL042W - - Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm biological_process molecular_function cytoplasm BY4742 [3-1] D 6 - cytoplasm cytoplasm cytoplasm - - - - [3-1] D 7 YCL044C MGR1 - Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA proteolysis involved in cellular protein catabolic process other membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [3-1] D 8 YCL045C EMC1 - Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 protein folding molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [3-1] D 9 YCL046W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C biological_process molecular_function cellular_component BY4742 [3-1] D 10 YCL047C POF1 nicotinamide-nucleotide adenylyltransferase Nicotinamide mononucleotide-specific adenylyltransferase (NMNAT); catalyzes the conversion of nicotinamide mononucleotide (NMN) to nicotinamide adenine dinucleotide (NAD+); role in the nicotinamide riboside (NR) salvage pathway of NAD+ biosynthesis; involved in NR and NAD+ homeostasis; ATPase involved in protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion response to chemical, cofactor metabolic process, invasive growth in response to glucose limitation, proteolysis involved in cellular protein catabolic process, pseudohyphal growth, nucleobase-containing small molecule metabolic process transferase activity, nucleotidyltransferase activity, hydrolase activity, ATPase activity nucleus, cytoplasm BY4742 [3-1] D 11 YCL048W SPS22 - Protein of unknown function; SPS22 has a paralog, SPS2, that arose from the whole genome duplication; redundant with Sps2p for the organization of the beta-glucan layer of the spore wall meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function membrane, plasma membrane, cytoplasm BY4742 [3-1] D 12 YCL049C - - Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene biological_process molecular_function membrane BY4742 [3-1] E 1 YCL050C APA1 bifunctional AP-4-A phosphorylase/ADP sulfurylase|DTP1 AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication nucleobase-containing small molecule metabolic process transferase activity, nucleotidyltransferase activity, hydrolase activity nucleus, cytoplasm BY4742 [3-1] E 2 YCL051W LRE1 - Protein involved in control of cell wall structure and stress response; direct inhibitor of the nuclear Dbf2 related (NDR) kinase Cbk1p-Mob2p; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; LRE1 has a paralog, HLR1, that arose from the whole genome duplication regulation of protein modification process, protein phosphorylation, cell wall organization or biogenesis enzyme regulator activity cellular bud, site of polarized growth BY4742 [3-1] E 3 - cellular bud, site of polarized growth cellular bud, site of polarized growth cellular bud, site of polarized growth - - - - [3-1] E 4 - - - - - - - - [3-1] E 5 - - - - - - - - [3-1] E 6 YCL055W KAR4 - Transcription factor required for response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone meiotic cell cycle, response to chemical, organelle fission, organelle fusion, conjugation, transcription from RNA polymerase II promoter, nucleus organization nucleic acid binding transcription factor activity nucleus, cytoplasm BY4742 [3-1] E 7 YCL056C PEX34 - Protein that regulates peroxisome populations; peroxisomal integral membrane protein; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation peroxisome organization molecular_function peroxisome, membrane, cytoplasm BY4742 [3-1] E 8 YCL057W PRD1 metalloendopeptidase Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress other peptidase activity, hydrolase activity endomembrane system, vacuole, mitochondrion, mitochondrial envelope, Golgi apparatus, cytoplasm BY4742 [3-1] E 9 - endomembrane system, vacuole, mitochondrion, mitochondrial envelope, Golgi apparatus, cytoplasm endomembrane system, vacuole, mitochondrion, mitochondrial envelope, Golgi apparatus, cytoplasm endomembrane system, vacuole, mitochondrion, mitochondrial envelope, Golgi apparatus, cytoplasm - - - - [3-1] E 10 - - - - - - - - [3-1] E 11 YCL060C - - - - - - BY4742 [3-1] E 12 YCL061C MRC1 chromatin-modulating protein MRC1|YCL060C S-phase checkpoint protein required for DNA replication; couples DNA helicase and polymerase; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; defines a novel S-phase checkpoint with Hog1p that coordinates DNA replication and transcription upon osmostress; protects uncapped telomeres; Dia2p-dependent degradation mediates checkpoint recovery; mammalian claspin homolog mitotic cell cycle, DNA repair, telomere organization, organelle fission, chromatin organization, DNA replication, cellular response to DNA damage stimulus, chromosome segregation, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, chromosome BY4742 [3-1] F 1 YCL062W nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - BY4742 [3-1] F 2 YCL063W VAC17 YCL062W Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p; involved in regulation of asymmetric inheritance of aggregated/misfolded proteins and age reset vacuole organization, organelle inheritance other membrane, vacuole, cytoplasm BY4742 [3-1] F 3 YCL064C CHA1 L-serine/L-threonine ammonia-lyase CHA1 Catabolic L-serine (L-threonine) deaminase; catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine cellular amino acid metabolic process lyase activity mitochondrion, cytoplasm BY4742 [3-1] F 4 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [3-1] F 5 - - - - - - - - [3-1] F 6 - - - - - - - - [3-1] F 7 - - - - - - - - [3-1] F 8 YCL069W VBA3 basic amino acid transporter Permease of basic amino acids in the vacuolar membrane; VBA3 has a paralog, VBA5, that arose from a segmental duplication transmembrane transport, ion transport, amino acid transport transmembrane transporter activity endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [3-1] F 9 - endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm - - - - [3-1] F 10 - - - - - - - - [3-1] F 11 - - - - - - - - [3-1] F 12 - - - - - - - - [3-1] G 1 YCR001W - - Putative protein of unknown function; conserved among S. cerevisiae strains; YCR001W is not an essential gene biological_process molecular_function cellular_component BY4742 [3-1] G 2 YCR002C CDC10 septin CDC10 Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress mitotic cell cycle, organelle fission, cytoskeleton organization, sporulation, cytokinesis, organelle assembly, protein complex biogenesis GTPase activity, hydrolase activity, ion binding, lipid binding, structural molecule activity cell cortex, cytoskeleton, cellular bud, membrane, site of polarized growth, cytoplasm BY4742 [3-1] G 3 YCR003W MRPL32 mitochondrial 54S ribosomal protein YmL32|YmL32 Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress mitochondrial translation, mitochondrion organization structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [3-1] G 4 YCR004C YCP4 flavodoxin-like fold family protein Protein of unknown function; has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process other membrane, mitochondrion, plasma membrane, cytoplasm BY4742 [3-1] G 5 YCR005C CIT2 citrate (Si)-synthase CIT2 Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle; catalyzes condensation of acetyl coenzyme A and oxaloacetate to form citrate; expression is controlled by Rtg1p and Rtg2p transcription factors; SCF-Ucc1 regulates level of Cit2p to maintain citrate homeostasis; oxaloacetate-dependent positive feedback loop inhibits Cit2p ubiquitination; CIT2 has a paralog, CIT1, that arose from the whole genome duplication carbohydrate metabolic process, monocarboxylic acid metabolic process, cellular amino acid metabolic process transferase activity peroxisome, mitochondrion, cytoplasm BY4742 [3-1] G 6 YCR006C - - Putative protein of unknown function; conserved among S. cerevisiae strains; YCR006C is not an essential gene biological_process molecular_function cellular_component BY4742 [3-1] G 7 YCR007C - DUP240 family protein Putative integral membrane protein; member of DUP240 gene family; SWAT-GFP and mCherry fusion proteins localize to the cell periphery and vacuole; YCR007C is not an essential gene biological_process molecular_function vacuole, cytoplasm BY4742 [3-1] G 8 YCR008W SAT4 serine/threonine protein kinase SAT4|HAL4 Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; overexpression affects the Fe-S and lipoamide containing proteins in the mitochondrion; required for lipoylation of Lat1p, Kgd2p and Gcv3p; partially redundant with Hal5p; has similarity to Npr1p; localizes to the cytoplasm and mitochondrion mitotic cell cycle, cofactor metabolic process, protein phosphorylation, protein dephosphorylation, cellular ion homeostasis kinase activity, transferase activity mitochondrion, cytoplasm BY4742 [3-1] G 9 YCR009C RVS161 amphiphysin-like protein RVS161|SPE161|FUS7|END6 Amphiphysin-like lipid raft protein; N-BAR domain protein that interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress cytoskeleton organization, conjugation, response to starvation, response to osmotic stress, endocytosis lipid binding, cytoskeletal protein binding cell cortex, cytoskeleton, membrane, site of polarized growth, cytoplasm BY4742 [3-1] G 10 YCR010C ADY2 ATO1 Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm BY4742 [3-1] G 11 YCR011C ADP1 putative ATP-dependent permease ADP1 Putative ATP-dependent permease of the ABC transporter family transmembrane transport hydrolase activity, transmembrane transporter activity, ATPase activity endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [3-1] G 12 - endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm - - - - [3-1] H 1 - - - - - - - - [3-1] H 2 YCR014C POL4 DNA-directed DNA polymerase IV|POLX DNA polymerase IV; undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta DNA repair, cellular response to DNA damage stimulus transferase activity, nucleotidyltransferase activity nucleus BY4742 [3-1] H 3 YCR015C CTO1 - Protein required for cold tolerance; involved in phosphate uptake; YCR015C is not an essential gene other molecular_function cellular_component BY4742 [3-1] H 4 YCR016W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis biological_process molecular_function nucleus, nucleolus BY4742 [3-1] H 5 YCR017C CWH43 - GPI lipid remodelase; responsible for introducing ceramides into GPI anchors having a C26:0 fatty acid in sn-2 of the glycerol moiety; can also use lyso-GPI protein anchors and various base resistant lipids as substrates; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion lipid metabolic process, cell wall organization or biogenesis, protein lipidation molecular_function endomembrane system, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [3-1] H 6 - endomembrane system, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm, plasma membrane - - - - [3-1] H 7 YCR019W MAK32 - Protein necessary for stability of L-A dsRNA-containing particles other molecular_function cellular_component BY4742 [3-1] H 8 YCR020C PET18 HIT2 Protein of unknown function; has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin vitamin metabolic process molecular_function cytoplasm BY4742 [3-1] H 9 YCR020C-A MAK31 NAA38|SMX1|LSM9 Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family protein acylation transferase activity cytoplasm BY4742 [3-1] H 10 YCR021C HSP30 YRO1 Negative regulator of the H(+)-ATPase Pma1p; stress-responsive protein; hydrophobic plasma membrane localized; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase response to heat, response to chemical, cellular response to DNA damage stimulus, response to oxidative stress, response to osmotic stress molecular_function endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [3-1] H 11 YCR022C - - Putative protein of unknown function; conserved among S. cerevisiae strains; YCR022C is not an essential gene biological_process molecular_function cellular_component BY4742 [3-1] H 12 YCR023C - - Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [4-1] A 1 YDL001W RMD1 - Cytoplasmic protein required for sporulation biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [4-1] A 2 YDL002C NHP10 HMO2 Non-essential INO80 chromatin remodeling complex subunit; preferentially binds DNA ends, protecting them from exonucleatic cleavage; deletion affects telomere maintenance via recombination; related to mammalian high mobility group proteins DNA repair, telomere organization, chromatin organization, cellular response to DNA damage stimulus, DNA recombination DNA binding nucleus, chromosome BY4742 [4-1] A 3 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [4-1] A 4 - - - - - - - - [4-1] A 5 YDL005C MED2 - Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; relocalizes to the cytosol in response to hypoxia DNA-templated transcription, initiation, transcription from RNA polymerase II promoter transcription factor activity, protein binding, transcription factor binding nucleus, cytoplasm BY4742 [4-1] A 6 YDL006W PTC1 type 2C protein phosphatase PTC1|TPD1|KCS2|CWH47 Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation response to chemical, RNA splicing, protein phosphorylation, protein dephosphorylation, mitochondrion organization, regulation of protein modification process, tRNA processing, conjugation, organelle inheritance, signaling, response to osmotic stress phosphatase activity, hydrolase activity nucleus, cytoplasm BY4742 [4-1] A 7 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-1] A 8 - - - - - - - - [4-1] A 9 YDL009C - - Protein of unknown function; mRNA identified as translated by ribosome profiling data; SWAT-GFP and mCherry fusion proteins localize to the cytosol; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene biological_process molecular_function cytoplasm BY4742 [4-1] A 10 YDL010W GRX6 glutathione-disulfide reductase GRX6 Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication response to chemical, response to oxidative stress oxidoreductase activity, ion binding endomembrane system, vacuole, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4742 [4-1] A 11 YDL011C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W biological_process molecular_function cellular_component BY4742 [4-1] A 12 YDL012C - - Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication biological_process molecular_function cellular bud, membrane, plasma membrane BY4742 [4-1] B 1 YDL013W SLX5 SUMO-targeted ubiquitin ligase complex subunit SLX5|ULS2|HEX3 Subunit of the Slx5-Slx8 SUMO-targeted Ub ligase (STUbL) complex; role in Ub-mediated degradation of histone variant Cse4p preventing mislocalization to euchromatin; role in proteolysis of spindle positioning protein Kar9p, and DNA repair proteins Rad52p and Rad57p; forms SUMO-dependent nuclear foci, including DNA repair centers; contains a RING domain and two SIM motifs; associates with the centromere; required for maintenance of genome integrity like human ortholog RNF4 peptidyl-amino acid modification, telomere organization, cellular response to DNA damage stimulus, proteolysis involved in cellular protein catabolic process, protein modification by small protein conjugation or removal transferase activity, ubiquitin-like protein binding nucleus, chromosome BY4742 [4-1] B 2 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [4-1] B 3 - - - - - - - - [4-1] B 4 - - - - - - - - [4-1] B 5 - - - - - - - - [4-1] B 6 YDL018C ERP3 - Protein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport other molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-1] B 7 YDL019C OSH2 oxysterol-binding protein related protein OSH2 Member of an oxysterol-binding protein family with seven members; in S. cerevisiae, family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; contains FFAT motif; interacts with ER anchor Scs2p at patches at the plasma membrane and at the nuclear envelope; regulated by sterol binding; OSH2 has a paralog, SWH1, that arose from the whole genome duplication lipid transport, exocytosis, endocytosis lipid binding, ion binding nucleus, cell cortex, endomembrane system, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [4-1] B 8 YDL020C RPN4 stress-regulated transcription factor RPN4|UFD5|SON1 Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress DNA repair, response to chemical, cellular response to DNA damage stimulus, proteolysis involved in cellular protein catabolic process, regulation of DNA metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [4-1] B 9 YDL021W GPM2 phosphoglycerate mutase family protein GPM2 Homolog of Gpm1p phosphoglycerate mutase; converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional; GPM2 has a paralog, GPM3, that arose from the whole genome duplication biological_process molecular_function cytoplasm BY4742 [4-1] B 10 YDL022W GPD1 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1|OSR5|OSG1|HOR1|DAR1 NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases Ypk1p and Ypk2p, dephosphorylation increases catalytic activity; forms a heterodimer with Pnc1p to facilitate its peroxisomal import protein targeting, cofactor metabolic process, transmembrane transport, peroxisome organization, nucleobase-containing small molecule metabolic process, response to osmotic stress oxidoreductase activity peroxisome, nucleus, mitochondrion, cytoplasm BY4742 [4-1] B 11 YDL023C - SRF4 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance biological_process molecular_function cellular_component BY4742 [4-1] B 12 YDL024C DIA3 putative acid phosphatase DIA3 Protein of unknown function; involved in invasive and pseudohyphal growth invasive growth in response to glucose limitation, pseudohyphal growth phosphatase activity, hydrolase activity cell wall BY4742 [4-1] C 1 YDL025C RTK1 putative serine/threonine protein kinase RTK1 Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress biological_process kinase activity, transferase activity cellular_component BY4742 [4-1] C 2 YDL026W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-1] C 3 YDL027C MRX9 - Protein that associates with mitochondrial ribosome; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene biological_process molecular_function endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [4-1] C 4 - endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm - - - - [4-1] C 5 - - - - - - - - [4-1] C 6 - - - - - - - - [4-1] C 7 - - - - - - - - [4-1] C 8 YDL032W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene biological_process molecular_function cellular_component BY4742 [4-1] C 9 YDL033C SLM3 tRNA-5-taurinomethyluridine 2-sulfurtransferase|MTU1|MTO2 tRNA-specific 2-thiouridylase; responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human homolog TRMU is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF), and can complement yeast null mutant tRNA processing, mitochondrion organization, RNA modification transferase activity mitochondrion, cytoplasm BY4742 [4-1] C 10 YDL034W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene biological_process molecular_function cellular_component BY4742 [4-1] C 11 YDL035C GPR1 - Plasma membrane G protein coupled receptor (GPCR); interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis response to chemical, invasive growth in response to glucose limitation, pseudohyphal growth, signaling signal transducer activity membrane, plasma membrane BY4742 [4-1] C 12 YDL036C PUS9 pseudouridine synthase PUS9 Mitochondrial tRNA:pseudouridine synthase; catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence; PUS9 has a paralog, RIB2, that arose from the whole genome duplication tRNA processing, RNA modification isomerase activity nucleus, mitochondrion, cytoplasm BY4742 [4-1] D 1 YDL037C BSC1 - Protein of unconfirmed function; similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; in W303 strain one continuous open reading frame comprising of YDL037C, the intergenic region and YDL039C encodes the gene IMI1 biological_process molecular_function cellular_component BY4742 [4-1] D 2 YDL038C cellular_component cellular_component cellular_component - - - BY4742 [4-1] D 3 YDL039C PRM7 pheromone-regulated protein PRM7|YDL038C Pheromone-regulated protein; predicted to have one transmembrane segment; promoter contains Gcn4p binding elements; in W303 strain one continuous open reading frame comprising of YDL037C, the intergenic region and YDL039C encodes the IMI1 conjugation molecular_function membrane BY4742 [4-1] D 4 YDL040C NAT1 peptide alpha-N-acetyltransferase complex A subunit NAT1|NAA15|AAA1 Subunit of protein N-terminal acetyltransferase NatA; NatA comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins to influence multiple processes such as cell cycle progression, heat-shock resistance, mating, sporulation, telomeric silencing and early stages of mitophagy; orthologous to human NAA15; expression of both human NAA10 and NAA15 functionally complements ard1 nat1 double mutant although single mutations are not complemented by their orthologs protein acylation transferase activity ribosome, mitochondrion, cytoplasm BY4742 [4-1] D 5 YDL041W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C biological_process molecular_function cellular_component BY4742 [4-1] D 6 YDL042C SIR2 NAD-dependent histone deacetylase SIR2|MAR1 Conserved NAD+ dependent histone deacetylase of the Sirtuin family; deacetylation targets are primarily nuclear proteins; required for telomere hypercluster formation in quiescent yeast cells; involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and rDNA; negatively regulates initiation of DNA replication; functions as regulator of autophagy like mammalian homolog SIRT1, and also of mitophagy chromatin organization, DNA replication, chromosome segregation, DNA recombination, regulation of DNA metabolic process chromatin binding, hydrolase activity nucleus, chromosome, nucleolus BY4742 [4-1] D 7 - nucleus, chromosome, nucleolus nucleus, chromosome, nucleolus nucleus, chromosome, nucleolus - - - - [4-1] D 8 YDL044C MTF2 NAM1 Mitochondrial protein that interacts with mitochondrial RNA polymerase; interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription cytoplasmic translation, mRNA processing RNA binding mitochondrion, cytoplasm BY4742 [4-1] D 9 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [4-1] D 10 YDL045W-A MRP10 mitochondrial 37S ribosomal protein YmS-T|YmS-T Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs; oxidized by Mia40p during import into mitochondria mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [4-1] D 11 YDL046W NPC2 sterol transporter Sterol transport protein and functional homolog of human NPC2/He1; human NPC2 is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes; yeast NPC2 can complement mutations in human NPC2 lipid transport molecular_function vacuole, cytoplasm BY4742 [4-1] D 12 - vacuole, cytoplasm vacuole, cytoplasm vacuole, cytoplasm - - - - [4-1] E 1 YDL048C STP4 - Protein containing a Kruppel-type zinc-finger domain; similar to Stp1p, Stp2p; predicted transcription factor; relative distribution to the nucleus increases upon DNA replication stress; STP4 has a paralog, STP3, that arose from the whole genome duplication biological_process DNA binding nucleus, mitochondrion, cytoplasm BY4742 [4-1] E 2 YDL049C KNH1 - Protein with similarity to Kre9p; Kre9p is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance carbohydrate metabolic process molecular_function cell wall, extracellular region, vacuole, cytoplasm BY4742 [4-1] E 3 YDL050C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-1] E 4 YDL051W LHP1 YLA1|LAH1 RNA binding protein required for maturation of tRNA and U6 snRNA; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen tRNA processing RNA binding nucleus, nucleolus BY4742 [4-1] E 5 YDL052C SLC1 1-acylglycerol-3-phosphate O-acyltransferase SLC1 1-acyl-sn-glycerol-3-phosphate acyltransferase; catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes lipid metabolic process transferase activity endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-1] E 6 YDL053C PBP4 - Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus, cytoplasm BY4742 [4-1] E 7 YDL054C MCH1 - Protein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport other other membrane, vacuole, cytoplasm BY4742 [4-1] E 8 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [4-1] E 9 YDL056W MBP1 transcription factor MBP1 Transcription factor; involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes mitotic cell cycle nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [4-1] E 10 YDL057W - - Putative protein of unknown function; YDL057W is not an essential gene biological_process molecular_function cellular_component BY4742 [4-1] E 11 - cellular_component cellular_component cellular_component - - - - [4-1] E 12 YDL059C RAD59 - Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; regulates replication fork progression in DNA ligase I-deficient cells; paralog of Rad52p DNA repair, telomere organization, cellular response to DNA damage stimulus, DNA recombination, regulation of DNA metabolic process other nucleus, cytoplasm BY4742 [4-1] F 1 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-1] F 2 YDL061W - - - - - - BY4742 [4-1] F 3 YDL062W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential biological_process molecular_function cellular_component BY4742 [4-1] F 4 YDL063C SYO1 - Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; binds to nascent Rpl5p during translation; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus protein targeting, ribosomal large subunit biogenesis, nuclear transport unfolded protein binding nucleus, cytoplasm BY4742 [4-1] F 5 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-1] F 6 YDL065C PEX19 PAS12 Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning protein targeting, transmembrane transport, peroxisome organization, organelle inheritance other peroxisome, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [4-1] F 7 YDL066W IDP1 isocitrate dehydrogenase (NADP(+)) IDP1 Mitochondrial NADP-specific isocitrate dehydrogenase; catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4742 [4-1] F 8 YDL067C COX9 cytochrome c oxidase subunit VIIa Subunit VIIa of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [4-1] F 9 YDL068W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-1] F 10 YDL069C CBS1 - Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other ribosome, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [4-1] F 11 YDL070W BDF2 - Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress; BDF2 has a paralog, BDF1, that arose from the whole genome duplication chromatin organization, regulation of organelle organization histone binding, DNA binding, transcription factor binding nucleus BY4742 [4-1] F 12 YDL071C - IRC1 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W biological_process molecular_function cellular_component BY4742 [4-1] G 1 YDL072C YET3 - Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-1] G 2 YDL073W AHK1 - Scaffold protein in the HKR1 sub-branch of the Hog1p-signaling pathway; physically interacts with the cytoplasmic domain of Hkr1p, and with Sho1p, Pbs2p, and Ste11p; prevents cross-talk signaling from Hkr1p of the osmotic stress MAPK cascade to the Kss1p MAPK cascade; non-essential gene regulation of protein modification process, protein phosphorylation, signaling, response to osmotic stress enzyme binding, structural molecule activity cellular_component BY4742 [4-1] G 3 YDL074C BRE1 E3 ubiquitin-protein ligase BRE1 E3 ubiquitin ligase; forms heterodimer with Rad6p to regulate K63 polyubiquitination in response to oxidative stress and to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control; interacts with RNA-binding protein Npl3p, linking histone ubiquitination to mRNA processing; Bre1p-dependent histone ubiquitination promotes pre-mRNA splicing mitotic cell cycle, meiotic cell cycle, organelle fission, chromatin organization, DNA replication, DNA recombination, transcription from RNA polymerase II promoter, DNA repair, telomere organization, cellular response to DNA damage stimulus, histone modification, protein modification by small protein conjugation or removal, regulation of DNA metabolic process, regulation of cell cycle transferase activity, DNA binding nucleus, chromosome BY4742 [4-1] G 4 YDL075W RPL31A eL31|ribosomal 60S subunit protein L31A|L31e|YL28|L34A|L31A|RPL34 Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication cytoplasmic translation, regulation of translation, translational elongation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [4-1] G 5 YDL076C RXT3 - Component of the Rpd3L histone deacetylase complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress transcription from RNA polymerase I promoter molecular_function nucleus, chromosome BY4742 [4-1] G 6 YDL077C VAM6 VPS39|VPL22|VPL18|CVT4 Guanine nucleotide exchange factor for the GTPase Gtr1p; subunit of the HOPS endocytic tethering complex; vacuole membrane protein; functions as a Rab GTPase effector, interacting with both GTP- and GDP-bound conformations of Ypt7p; facilitates tethering and promotes membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; component of vacuole-mitochondrion contacts (vCLAMPs) important for lipid transfer between organelles vacuole organization, regulation of organelle organization, regulation of transport, organelle fusion, vesicle organization, protein complex biogenesis, membrane fusion, signaling guanyl-nucleotide exchange factor activity, lipid binding, enzyme binding, ion binding vacuole, membrane, cytoplasm BY4742 [4-1] G 7 YDL078C MDH3 malate dehydrogenase MDH3 Peroxisomal malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle cofactor metabolic process, monocarboxylic acid metabolic process, lipid metabolic process, nucleobase-containing small molecule metabolic process RNA binding, oxidoreductase activity, mRNA binding peroxisome, cytoplasm BY4742 [4-1] G 8 YDL079C MRK1 putative serine/threonine protein kinase MRK1 Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation; MRK1 has a paralog, RIM11, that arose from the whole genome duplication response to heat, protein phosphorylation, response to osmotic stress kinase activity, transferase activity cellular_component BY4742 [4-1] G 9 YDL080C THI3 branched-chain-2-oxoacid decarboxylase THI3|KID1 Regulatory protein that binds Pdc2p and Thi2p transcription factors; activates thiamine biosynthesis transcription factors Pdc2p and Thi2p by binding to them, but releases and de-activates them upon binding to thiamine pyrophosphate (TPP), the end product of the pathway; has similarity to decarboxylases but enzymatic activity is not detected vitamin metabolic process, transcription from RNA polymerase II promoter transcription factor binding nucleus BY4742 [4-1] G 10 YDL081C RPP1A P1A|ribosomal protein P1A|RPLA1 Ribosomal stalk protein P1 alpha; involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component cytoplasmic translation, regulation of protein modification process, protein phosphorylation structural molecule activity, structural constituent of ribosome, enzyme regulator activity ribosome, cytoplasm BY4742 [4-1] G 11 YDL082W RPL13A eL13|ribosomal 60S subunit protein L13A|L13e|L13A Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [4-1] G 12 YDL083C RPS16B uS9|ribosomal 40S subunit protein S16B|S9|rp61R|S16B Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis, DNA recombination structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [4-1] H 1 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [4-1] H 2 YDL085W NDE2 NADH-ubiquinone reductase (H(+)-translocating) NDE2|NDH2 Mitochondrial external NADH dehydrogenase; catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain; NDE2 has a paralog, NDE1, that arose from the whole genome duplication carbohydrate metabolic process, cofactor metabolic process, nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, monocarboxylic acid metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4742 [4-1] H 3 YDL086W - carboxymethylenebutenolidase Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene biological_process hydrolase activity mitochondrion, cytoplasm BY4742 [4-1] H 4 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [4-1] H 5 YDL088C ASM4 FG-nucleoporin ASM4|NUP59 FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication response to heat, nucleobase-containing compound transport, nuclear transport, nucleus organization lipid binding, DNA binding, ion binding nucleus, endomembrane system, membrane BY4742 [4-1] H 6 YDL089W NUR1 - Protein involved in regulation of mitotic exit; dephosphorylation target of Cdc14p in anaphase, which promotes timely rDNA segregation and allows mitotic progression; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate mitotic cell cycle, organelle fission, regulation of organelle organization, regulation of cell cycle molecular_function nucleus BY4742 [4-1] H 7 YDL090C RAM1 protein farnesyltransferase|STE16|SGP2|SCG2|FUS8|DPR1 Beta subunit of the CAAX farnesyltransferase (FTase); this complex prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit other transferase activity cytoplasm BY4742 [4-1] H 8 YDL091C UBX3 - Clathrin-coated vesicle component, regulator of endocytosis; copurifies with the DSC ubiquitin ligase complex; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p; required for efficient clathrin-mediated endocytosis; ortholog of fission yeast Ucp10 chromatin organization, histone modification, protein modification by small protein conjugation or removal, endocytosis other cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [4-1] H 9 - cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, cytoplasm cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, cytoplasm cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, cytoplasm - - - - [4-1] H 10 YDL093W PMT5 putative dolichyl-phosphate-mannose-protein mannosyltransferase PMT5 Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals protein glycosylation transferase activity, transferring glycosyl groups, transferase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-1] H 11 YDL094C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential biological_process molecular_function cellular_component BY4742 [4-1] H 12 YDL095W PMT1 dolichyl-phosphate-mannose-protein mannosyltransferase PMT1 Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen response to chemical, proteolysis involved in cellular protein catabolic process, protein glycosylation, signaling transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [4-2] A 1 YDL096C OPI6 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential biological_process molecular_function cellular_component BY4742 [4-2] A 2 - cellular_component cellular_component cellular_component - - - - [4-2] A 3 - - - - - - - - [4-2] A 4 YDL099W BUG1 - Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes Golgi vesicle transport molecular_function cytoplasm BY4742 [4-2] A 5 YDL100C GET3 guanine nucleotide exchange factor GET3|ARR4 Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; functions as a chaperone under ATP-depleted oxidative stress conditions; subunit of GET complex, involved in ATP dependent Golgi to ER trafficking and insertion of tail-anchored (TA) proteins into ER membrane under non-stress conditions; binds as dimer to transmembrane domain (TMD) cargo, shielding TMDs from aqueous solvent; protein abundance increases under DNA replication stress response to heat, response to chemical, protein targeting, Golgi vesicle transport, protein folding, conjugation, signaling guanyl-nucleotide exchange factor activity, hydrolase activity, unfolded protein binding, ATPase activity endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-2] A 6 YDL101C DUN1 serine/threonine protein kinase DUN1 Cell-cycle checkpoint S/T protein kinase; required for transient G2/M arrest after DNA damage, damage-induced transcription, and nuclear-to-cytoplasmic redistribution of Rnr2p-Rnr4p after genotoxic stress and iron deprivation; phosphorylates repair protein Rad55p, transcriptional repressor Sml1p, superoxide dismutase, and ribonucleotide reductase inhibitors Crt1p and Dif1p; functions in the Mec1p pathway to regulate dNTP pools and telomere length; postreplicative repair role peptidyl-amino acid modification, protein phosphorylation, DNA replication, cellular response to DNA damage stimulus, regulation of DNA metabolic process, regulation of cell cycle kinase activity, transferase activity nucleus BY4742 [4-2] A 7 - nucleus nucleus nucleus - - - - [4-2] A 8 - - - - - - - - [4-2] A 9 YDL104C QRI7 putative N(6)-L-threonylcarbamoyladenine synthase Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; highly conserved mitochondrial protein; essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification; when directed to the cytoplasm, complements the essential function of Kae1p in the KEOPS complex tRNA processing, mitochondrion organization, RNA modification molecular_function mitochondrion, cytoplasm BY4742 [4-2] A 10 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [4-2] A 11 YDL106C PHO2 phoB|GRF10|BAS2 Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p; relocalizes to the cytosol in response to hypoxia chromatin organization, nucleobase-containing small molecule metabolic process, transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [4-2] A 12 YDL107W MSS2 - Peripherally bound inner membrane protein of the mitochondrial matrix; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-2] B 1 - membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm - - - - [4-2] B 2 YDL109C - putative lipase Putative lipase; involved in lipid metabolism; not an essential gene; YDL109C has a paralog, ROG1, that arose from the whole genome duplication lipid metabolic process molecular_function cellular_component BY4742 [4-2] B 3 YDL110C TMA17 ADC17 ATPase dedicated chaperone that adapts proteasome assembly to stress; Tma17p is induced upon stress; interacts with Rpt6p to assist its pairing to Rpt3p and early steps in proteasome biogenesis; associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion protein complex biogenesis protein binding, bridging nucleus, ribosome, cytoplasm BY4742 [4-2] B 4 - nucleus, ribosome, cytoplasm nucleus, ribosome, cytoplasm nucleus, ribosome, cytoplasm - - - - [4-2] B 5 YDL112W TRM3 tRNA (guanosine(18)-2'-O)-methyltransferase 2'-O-ribose methyltransferase; catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs tRNA processing, RNA modification transferase activity, methyltransferase activity cytoplasm BY4742 [4-2] B 6 YDL113C ATG20 CVT20|SNX42 Sorting nexin family member; required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate protein targeting, mitochondrion organization, Golgi vesicle transport, endosomal transport lipid binding, ion binding cytoplasmic vesicle, endomembrane system, membrane, cytoplasm BY4742 [4-2] B 7 YDL114W - short-chain dehydrogenase/reductase Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene biological_process molecular_function membrane BY4742 [4-2] B 8 YDL115C IWR1 - RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress protein targeting, nuclear transport other nucleus, cytoplasm BY4742 [4-2] B 9 YDL116W NUP84 - Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 response to heat, DNA repair, protein targeting, chromatin organization, cellular response to DNA damage stimulus, nucleobase-containing compound transport, transcription from RNA polymerase II promoter, nuclear transport, nucleus organization structural molecule activity nucleus, endomembrane system, membrane BY4742 [4-2] B 10 YDL117W CYK3 - SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants mitotic cell cycle, cytokinesis enzyme regulator activity nucleus, cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [4-2] B 11 YDL118W - - Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein biological_process molecular_function cellular_component BY4742 [4-2] B 12 YDL119C HEM25 - Mitochondrial glycine transporter; required for the transport of glycine into mitochondria for initiation of heme biosynthesis, with YMC1 acting as a secondary transporter; homolog of human SLC25A38, a mitochondrial glycine transporter associated with nonsyndromic autosomal recessive congenital sideroblastic anemia; human SLC25A38 can complement the heme deficiency associated with the null mutant; GFP-fusion protein is induced in response to the DNA-damaging agent MMS ion transport, amino acid transport transmembrane transporter activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-2] C 1 - membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm - - - - [4-2] C 2 YDL121C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDL121C is not an essential protein biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-2] C 3 YDL122W UBP1 ubiquitin-specific protease UBP1 Ubiquitin-specific protease; removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains protein modification by small protein conjugation or removal peptidase activity, hydrolase activity endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-2] C 4 YDL123W SNA4 - Protein of unknown function; localized to the vacuolar outer membrane; predicted to be palmitoylated biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [4-2] C 5 YDL124W - aldo-keto reductase superfamily protein NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress response to chemical, response to oxidative stress oxidoreductase activity nucleus, membrane, plasma membrane, cytoplasm BY4742 [4-2] C 6 YDL125C HNT1 adenosine 5'-monophosphoramidase Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of histidine triad (HIT) superfamily of nucleotide-binding proteins; protein abundance increases in response to DNA replication stress; human homolog HINT1 can complement yeast hnt1 mutant nucleobase-containing small molecule metabolic process hydrolase activity nucleus, cytoplasm BY4742 [4-2] C 7 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-2] C 8 YDL127W PCL2 cyclin PCL2|CLN4 Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth; PCL2 has a paralog, PCL9, that arose from the whole genome duplication protein phosphorylation, cytoskeleton organization, regulation of protein modification process, regulation of cell cycle enzyme regulator activity nucleus, cellular bud, site of polarized growth BY4742 [4-2] C 9 YDL128W VCX1 MNR1|HUM1 Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity; involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter cellular ion homeostasis, transmembrane transport, ion transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [4-2] C 10 YDL129W - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus, cytoplasm BY4742 [4-2] C 11 YDL130W RPP1B ribosomal protein P1B|YP1beta|P1B|L44'|Ax|RPLA3|RPL44' Ribosomal protein P1 beta; component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation is regulated by phosphorylation and interaction with the P2 stalk component cytoplasmic translation, regulation of protein modification process, protein phosphorylation structural molecule activity, structural constituent of ribosome, enzyme regulator activity vacuole, ribosome, cytoplasm BY4742 [4-2] C 12 YDL131W LYS21 homocitrate synthase LYS21 Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication cellular amino acid metabolic process transferase activity nucleus BY4742 [4-2] D 1 - nucleus nucleus nucleus - - - - [4-2] D 2 YDL133W SRF1 - Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients other molecular_function membrane, vacuole, cytoplasm BY4742 [4-2] D 3 YDL134C PPH21 phosphoprotein phosphatase 2A catalytic subunit PPH21 Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication mitotic cell cycle, regulation of translation, organelle fission, cell budding, protein dephosphorylation, cytoskeleton organization, regulation of organelle organization, chromosome segregation, regulation of cell cycle phosphatase activity, hydrolase activity nucleus, cytoplasm BY4742 [4-2] D 4 YDL134C-A nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - BY4742 [4-2] D 5 YDL135C RDI1 - Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress vacuole organization, cytoskeleton organization, regulation of organelle organization, organelle fusion, membrane fusion, signaling enzyme regulator activity cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane BY4742 [4-2] D 6 YDL136W RPL35B uL29|ribosomal 60S subunit protein L35B|L35B|L29|SOS2 Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal large subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [4-2] D 7 YDL137W ARF2 Arf family GTPase ARF2 ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6 other GTPase activity, hydrolase activity endomembrane system, cytoplasm, Golgi apparatus BY4742 [4-2] D 8 YDL138W RGT2 glucose sensor Plasma membrane high glucose sensor that regulates glucose transport; low affinity sesnor that contains 12 predicted transmembrane segments and a long C-terminal tail required for hexose transporter induction; phosphorylation of the tail by Yck1p/Yck2p facilitates binding to the HXT co-repressors, Mth1p and Std1p; RGT2 has a paralog, SNF3, that arose from the whole genome duplication response to chemical, signaling molecular_function membrane, plasma membrane BY4742 [4-2] D 9 - membrane, plasma membrane membrane, plasma membrane membrane, plasma membrane - - - - [4-2] D 10 - - - - - - - - [4-2] D 11 - - - - - - - - [4-2] D 12 YDL142C CRD1 cardiolipin synthase|CLS1 Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function and for correct integration of membrane-multispanning proteins into the mitochondrial outer membrane; required to maintain tubular mitochondrial morphology and functions in mitochondrial fusion; also required for normal vacuolar ion homeostasis protein targeting, mitochondrion organization, cellular ion homeostasis, lipid metabolic process transferase activity mitochondrion, membrane, cytoplasm BY4742 [4-2] E 1 - mitochondrion, membrane, cytoplasm mitochondrion, membrane, cytoplasm mitochondrion, membrane, cytoplasm - - - - [4-2] E 2 YDL144C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus, cytoplasm BY4742 [4-2] E 3 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-2] E 4 YDL146W LDB17 - Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck endocytosis molecular_function cellular bud, cell cortex, site of polarized growth, cytoskeleton, cytoplasm BY4742 [4-2] E 5 - cellular bud, cell cortex, site of polarized growth, cytoskeleton, cytoplasm cellular bud, cell cortex, site of polarized growth, cytoskeleton, cytoplasm cellular bud, cell cortex, site of polarized growth, cytoskeleton, cytoplasm - - - - [4-2] E 6 - - - - - - - - [4-2] E 7 YDL149W ATG9 autophagy protein ATG9|CVT6|APG9|CVT7|AUT9 Transmembrane protein involved in forming Cvt and autophagic vesicles; cycles between the phagophore assembly site (PAS) and other cytosolic punctate structures, not found in autophagosomes; may be involved in membrane delivery to the PAS vacuole organization, protein targeting, mitochondrion organization, organelle assembly, protein complex biogenesis molecular_function cytoplasmic vesicle, mitochondrion, membrane, cytoplasm BY4742 [4-2] E 8 - cytoplasmic vesicle, mitochondrion, membrane, cytoplasm cytoplasmic vesicle, mitochondrion, membrane, cytoplasm cytoplasmic vesicle, mitochondrion, membrane, cytoplasm - - - - [4-2] E 9 YDL151C BUD30 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay mitotic cell cycle, meiotic cell cycle, sporulation, cytokinesis, transcription from RNA polymerase II promoter molecular_function cellular_component BY4742 [4-2] E 10 - cellular_component cellular_component cellular_component - - - - [4-2] E 11 - - - - - - - - [4-2] E 12 YDL154W MSH5 MutS family protein MSH5 Protein of the MutS family; forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans meiotic cell cycle, organelle fission, DNA recombination molecular_function nucleus BY4742 [4-2] F 1 YDL155W CLB3 B-type cyclin CLB3 B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress; CLB3 has a paralog, CLB4, that arose from the whole genome duplication mitotic cell cycle, protein phosphorylation, organelle fission, cytoskeleton organization, regulation of organelle organization, organelle assembly, regulation of protein modification process, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm BY4742 [4-2] F 2 YDL156W CMR1 - Nuclear protein with a role in protein quality control; localizes to the intranuclear quality control compartment (INQ) in response to proteasome inhibition or DNA replication stress; INQ likely sequesters proteins involved in DNA metabolism for degradation or re-folding; DNA-binding protein with preference for UV-damaged DNA; contains three WD domains (WD-40 repeat); human ortholog WDR76 also exhibits perinuclear localization under similar stress conditions cellular response to DNA damage stimulus, regulation of cell cycle DNA binding nucleus, chromosome, cytoplasm BY4742 [4-2] F 3 YDL157C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [4-2] F 4 YDL158C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-2] F 5 YDL159W STE7 mitogen-activated protein kinase kinase STE7 Signal transducing MAP kinase kinase; involved in pheromone response where it phosphorylates Fus3p; involved in the pseudohyphal/invasive growth pathway where it phosphorylates of Kss1p; phosphorylated by Ste11p; degraded by ubiquitin pathway response to chemical, protein phosphorylation, invasive growth in response to glucose limitation, transposition, regulation of protein modification process, conjugation, pseudohyphal growth, cell wall organization or biogenesis, signaling kinase activity, transferase activity, signal transducer activity site of polarized growth, cytoplasm BY4742 [4-2] F 6 YDL160C DHH1 DExD/H-box ATP-dependent RNA helicase DHH1 Cytoplasmic DEAD-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacting with both decapping and deadenylase complexes; role in translational repression, mRNA decay, and possibly mRNA export; interacts and cooperates with Ngr1p to promote specific mRNA decay; ATP- and RNA-bound form promotes processing body (PB) assembly, while ATPase stimulation by Not1p promotes PB disassembly; forms cytoplasmic foci on replication stress regulation of translation, regulation of organelle organization, organelle assembly, translational elongation, RNA catabolic process chromatin binding, RNA binding, hydrolase activity, ATPase activity, helicase activity, mRNA binding membrane, cytoplasm BY4742 [4-2] F 7 YDL161W ENT1 epsin Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress; ENT1 has a paralog, ENT2, that arose from the whole genome duplication cytoskeleton organization, endocytosis ubiquitin-like protein binding cytoplasmic vesicle, endomembrane system, cellular bud, site of polarized growth, cytoplasm BY4742 [4-2] F 8 YDL162C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly biological_process molecular_function cellular_component BY4742 [4-2] F 9 - cellular_component cellular_component cellular_component - - - - [4-2] F 10 - - - - - - - - [4-2] F 11 - - - - - - - - [4-2] F 12 - - - - - - - - [4-2] G 1 YDL167C NRP1 - Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis biological_process molecular_function cytoplasm BY4742 [4-2] G 2 YDL168W SFA1 bifunctional alcohol dehydrogenase/S-(hydroxymethyl)glutathione dehydrogenase|ADH5 Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4742 [4-2] G 3 YDL169C UGX2 - Protein of unknown function; transcript accumulates in response to any combination of stress conditions biological_process molecular_function cellular_component BY4742 [4-2] G 4 YDL170W UGA3 - Transcriptional activator for GABA-dependent induction of GABA genes; binds to DNA elements found in the promoters of target genes and increases their expression in the presence of GABA (gamma-aminobutyrate); zinc finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus; examples of GABA genes include UGA1, UGA2, and UGA4 response to chemical, transcription from RNA polymerase II promoter, monocarboxylic acid metabolic process, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding nucleus BY4742 [4-2] G 5 YDL171C GLT1 glutamate synthase (NADH) NAD(+)-dependent glutamate synthase (GOGAT); synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source; assembles into filaments as cells approach stationary phase and under cytosolic acidification and starvation conditions cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4742 [4-2] G 6 YDL172C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-2] G 7 YDL173W PAR32 - Protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene biological_process molecular_function cytoplasm BY4742 [4-2] G 8 YDL174C DLD1 D-lactate dehydrogenase Major mitochondrial D-lactate dehydrogenase; oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane monocarboxylic acid metabolic process oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-2] G 9 YDL175C AIR2 TRAMP complex RNA-binding subunit RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication; Air2p and Air1p have nonredundant roles in regulation of substrate specificity of the exosome RNA catabolic process, tRNA processing, RNA modification protein binding, bridging, RNA binding, transferase activity, nucleotidyltransferase activity, hydrolase activity, ATPase activity, helicase activity nucleus, nucleolus BY4742 [4-2] G 10 YDL176W - - Protein of unknown function; predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene biological_process molecular_function cellular_component BY4742 [4-2] G 11 YDL177C - - Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene biological_process molecular_function cellular_component BY4742 [4-2] G 12 YDL178W DLD2 D-lactate dehydrogenase|AIP2 D-2-hydroxyglutarate dehydrogenase, and minor D-lactate dehydrogenase; mitochondrial matrix protein that oxidizes D-2-hydroxyglutarate (D-2HG), an oncometabolite, to alpha-ketoglutarate with a minor role in lactate catabolism; located in the mitochondrial matrix monocarboxylic acid metabolic process cytoskeletal protein binding, ion binding, oxidoreductase activity mitochondrion, cytoplasm BY4742 [4-2] H 1 YDL179W PCL9 - Cyclin; forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p; PCL9 has a paralog, PCL2, that arose from the whole genome duplication mitotic cell cycle enzyme regulator activity cellular bud, site of polarized growth BY4742 [4-2] H 2 YDL180W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [4-2] H 3 YDL181W INH1 ATPase inhibitor Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase; inhibitory function is enhanced by stabilizing proteins Stf1p and Stf2p; has a calmodulin-binding motif and binds calmodulin in vitro; INH1 has a paralog, STF1, that arose from the whole genome duplication other enzyme regulator activity mitochondrion, cytoplasm BY4742 [4-2] H 4 YDL182W LYS20 homocitrate synthase LYS20 Homocitrate synthase isozyme and functions in DNA repair; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication DNA repair, chromatin organization, cellular response to DNA damage stimulus, cellular amino acid metabolic process transferase activity nucleus, mitochondrion, cytoplasm BY4742 [4-2] H 5 YDL183C - MKR1 Protein that may form an active mitochondrial KHE system; mitochondrial inner-membrane protein; non-essential gene; KHE system stands for K+/H+ exchanger system ion transport molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-2] H 6 YDL184C RPL41A eL41|ribosomal 60S subunit protein L41A|L41e|YL41|L47A|L41A|RPL47A Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [4-2] H 7 YDL185W VMA1 H(+)-transporting V1 sector ATPase subunit A|TFP1|CLS8 Subunit A of the V1 peripheral membrane domain of V-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits; involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner cellular ion homeostasis RNA binding, hydrolase activity, transmembrane transporter activity, ATPase activity, mRNA binding vacuole, membrane, cytoplasm BY4742 [4-2] H 8 YDL186W - - Putative protein of unknown function; YDL186W is not an essential gene biological_process molecular_function cellular_component BY4742 [4-2] H 9 YDL187C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-2] H 10 YDL188C PPH22 phosphoprotein phosphatase 2A catalytic subunit PPH22|PPH2 Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; dephosphorylates Tel1p/Mec1p-phosphorylated Cdc13p to promote telomerase release from telomeres at G2/M; PPH22 has a paralog, PPH21, that arose from the whole genome duplication mitotic cell cycle, regulation of translation, organelle fission, cell budding, protein dephosphorylation, cytoskeleton organization, regulation of organelle organization, chromosome segregation, regulation of cell cycle phosphatase activity, hydrolase activity nucleus, chromosome BY4742 [4-2] H 11 YDL189W RBS1 - Protein involved in assembly of the RNA polymerase III (Pol III) complex; high copy suppressor of Pol III assembly mutation and psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; physically interacts with Pol III; proposed to bind single-stranded nucleic acids via its R3H domain carbohydrate metabolic process, protein complex biogenesis molecular_function nucleus, cytoplasm BY4742 [4-2] H 12 YDL190C UFD2 ubiquitin-ubiquitin ligase UFD2 Ubiquitin chain assembly factor (E4); cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 response to chemical, regulation of protein modification process, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process transferase activity nucleus, cytoplasm BY4742 [4-3] A 1 YDL191W RPL35A uL29|ribosomal 60S subunit protein L35A|L35A|L29|SOS1 Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal large subunit biogenesis structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [4-3] A 2 YDL192W ARF1 Arf family GTPase ARF1 ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6 Golgi vesicle transport GTPase activity, hydrolase activity endomembrane system, cytoplasm, Golgi apparatus BY4742 [4-3] A 3 - endomembrane system, cytoplasm, Golgi apparatus endomembrane system, cytoplasm, Golgi apparatus endomembrane system, cytoplasm, Golgi apparatus - - - - [4-3] A 4 - - - - - - - - [4-3] A 5 - - - - - - - - [4-3] A 6 - - - - - - - - [4-3] A 7 YDL197C ASF2 - Anti-silencing protein; causes derepression of silent loci when overexpressed chromatin organization molecular_function nucleus, chromosome BY4742 [4-3] A 8 YDL198C GGC1 SHM1|YHM1 Mitochondrial GTP/GDP transporter; essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family mitochondrion organization, transmembrane transport, nucleobase-containing compound transport, cellular ion homeostasis transmembrane transporter activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [4-3] A 9 YDL199C - - Putative transporter; member of the sugar porter family transmembrane transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [4-3] A 10 YDL200C MGT1 methylated-DNA--protein-cysteine methyltransferase|MGMT DNA repair methyltransferase (6-O-methylguanine-DNA methylase); involved in protection against DNA alkylation damage DNA repair, cellular response to DNA damage stimulus transferase activity, methyltransferase activity nucleus BY4742 [4-3] A 11 YDL201W TRM8 tRNA (guanine46-N7)-methyltransferase Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p tRNA processing, RNA modification transferase activity, methyltransferase activity nucleus, cytoplasm BY4742 [4-3] A 12 YDL202W MRPL11 mitochondrial 54S ribosomal protein YmL11|YmL11 Mitochondrial ribosomal protein of the large subunit; localizes to vacuole in response to H2O2 mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [4-3] B 1 YDL203C ACK1 - Protein that functions in the cell wall integrity pathway; functions upstream of Pkc1p; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria cell wall organization or biogenesis, signaling molecular_function mitochondrion, cytoplasm BY4742 [4-3] B 2 YDL204W RTN2 - Reticulon protein; involved in nuclear pore assembly and maintenance of tubular ER morphology; promotes membrane curvature; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; interacts with Sec6p, Yip3p, and Sbh1p; less abundant than RTN1; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress peroxisome organization, organelle inheritance molecular_function nucleus, cell cortex, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-3] B 3 - nucleus, cell cortex, endomembrane system, endoplasmic reticulum, cytoplasm nucleus, cell cortex, endomembrane system, endoplasmic reticulum, cytoplasm nucleus, cell cortex, endomembrane system, endoplasmic reticulum, cytoplasm - - - - [4-3] B 4 YDL206W - - Putative protein of unknown function; YDL206W is not an essential protein biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [4-3] B 5 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [4-3] B 6 - - - - - - - - [4-3] B 7 - - - - - - - - [4-3] B 8 YDL210W UGA4 - GABA (gamma-aminobutyrate) permease; serves as a GABA transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane transmembrane transport, ion transport, amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [4-3] B 9 YDL211C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YDL211C has a paralog, TDA7, that arose from the whole genome duplication biological_process molecular_function vacuole, cytoplasm BY4742 [4-3] B 10 - vacuole, cytoplasm vacuole, cytoplasm vacuole, cytoplasm - - - - [4-3] B 11 YDL213C NOP6 - rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes ribosomal small subunit biogenesis RNA binding, rRNA binding nucleus, nucleolus BY4742 [4-3] B 12 YDL214C PRR2 serine/threonine protein kinase PRR2 Serine/threonine protein kinase; inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance; PRR2 has a paralog, NPR1, that arose from the whole genome duplication response to chemical, protein phosphorylation, conjugation, transcription from RNA polymerase II promoter kinase activity, transferase activity cellular_component BY4742 [4-3] C 1 YDL215C GDH2 glutamate dehydrogenase (NAD(+))|GDHB|GDH-B NAD(+)-dependent glutamate dehydrogenase; degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels; genetically interacts with GDH3 by suppressing stress-induced apoptosis other oxidoreductase activity mitochondrion, cytoplasm BY4742 [4-3] C 2 YDL216C RRI1 COP9 signalosome catalytic subunit RRI1|JAB1|CSN5 Catalytic subunit of the COP9 signalosome (CSN) complex; acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling; involved in modulation of genes controlling amino acid and lipid metabolism, and ergosterol biosynthesis response to chemical, lipid metabolic process, protein modification by small protein conjugation or removal, conjugation, signaling peptidase activity, hydrolase activity nucleus BY4742 [4-3] C 3 - nucleus nucleus nucleus - - - - [4-3] C 4 YDL218W - - Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin biological_process molecular_function membrane, cytoplasm BY4742 [4-3] C 5 YDL219W DTD1 D-tyrosyl-tRNA(Tyr) deacylase D-Tyr-tRNA(Tyr) deacylase; functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes cellular amino acid metabolic process hydrolase activity cytoplasm BY4742 [4-3] C 6 - cytoplasm cytoplasm cytoplasm - - - - [4-3] C 7 - - - - - - - - [4-3] C 8 YDL222C FMP45 - Integral membrane protein localized to mitochondria; required for sporulation and maintaining sphingolipid content; similar to SUR7; FMP45 has a paralog, YNL194C, that arose from the whole genome duplication meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function cell cortex, mitochondrion, membrane, cytoplasm, plasma membrane BY4742 [4-3] C 9 YDL223C HBT1 - Shmoo tip protein, substrate of Hub1p ubiquitin-like protein; mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis; HBT1 has a paralog, YNL195C, that arose from the whole genome duplication response to chemical, cell morphogenesis, conjugation molecular_function membrane, plasma membrane BY4742 [4-3] C 10 YDL224C WHI4 - Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication regulation of translation RNA binding cytoplasm BY4742 [4-3] C 11 YDL225W SHS1 septin SHS1|SEP7 Component of the septin ring that is required for cytokinesis; present at the ends of rod-like septin hetero-oligomers; C-terminal extension is important for recruitment of Bni5p to the mother-bud neck, which in turn is required for Myo1p recruitment and cytokinesis; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress mitotic cell cycle, organelle fission, cytoskeleton organization, organelle assembly, cytokinesis, protein complex biogenesis ion binding cell cortex, cytoskeleton, cellular bud, membrane, site of polarized growth, cytoplasm BY4742 [4-3] C 12 YDL226C GCS1 - ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; required for prospore membrane formation; regulates phospholipase Spo14p; shares functional similarity with Glo3p; GCS1 has a paralog, SPS18, that arose from the whole genome duplication cytoskeleton organization, Golgi vesicle transport enzyme regulator activity, cytoskeletal protein binding cytoskeleton, cytoplasmic vesicle, endomembrane system, Golgi apparatus, cytoplasm BY4742 [4-3] D 1 YDL227C HO - Site-specific endonuclease; required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p, and Ash1p DNA recombination nuclease activity, hydrolase activity nucleus BY4742 [4-3] D 2 YDL229W SSB1 Hsp70 family ATPase SSB1|YG101 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication cytoplasmic translation, regulation of translation, rRNA processing, translational elongation, ribosomal subunit export from nucleus, protein folding, response to starvation, nuclear transport hydrolase activity, unfolded protein binding, ATPase activity membrane, cytoplasm, plasma membrane BY4742 [4-3] D 3 YDL230W PTP1 tyrosine protein phosphatase PTP1 Phosphotyrosine-specific protein phosphatase; dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation invasive growth in response to glucose limitation, protein dephosphorylation, pseudohyphal growth phosphatase activity, hydrolase activity mitochondrion, cytoplasm BY4742 [4-3] D 4 YDL231C BRE4 - Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport endocytosis molecular_function membrane BY4742 [4-3] D 5 YDL232W OST4 olichyl-diphosphooligosaccharide--protein glycotransferase OST4 Subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes protein asparagine-linked glycosylation; type I membrane protein required for incorporation of Ost3p or Ost6p into the OST complex protein glycosylation transferase activity, transferring glycosyl groups, protein binding, bridging, transferase activity endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm BY4742 [4-3] D 6 YDL233W MFG1 - Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene biological_process molecular_function nucleus, cytoplasm BY4742 [4-3] D 7 YDL234C GYP7 - GTPase-activating protein for yeast Rab family members; members include Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking; contains a PH-like domain vacuole organization, membrane fusion, regulation of organelle organization, organelle fusion enzyme regulator activity cytoplasm BY4742 [4-3] D 8 - cytoplasm cytoplasm cytoplasm - - - - [4-3] D 9 YDL236W PHO13 4-nitrophenylphosphatase Conserved phosphatase acting as a metabolite repair enzyme; shows specific dephosphorylating activity on two side-products of central carbohydrate metabolism, 2-phosphoglycolate and 4-phosphoerythronate; alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity; human ortholog PGP shows similar substrate specificity, deletion causes similar metabolite accumulation phenotypes, suggesting conserved role in eliminating glycolytic byproducts carbohydrate metabolic process, protein dephosphorylation phosphatase activity, hydrolase activity nucleus, cytoplasm BY4742 [4-3] D 10 YDL237W AIM6 LRC1 Protein of unknown function; required for respiratory growth; YDL237W is not an essential gene biological_process molecular_function cellular_component BY4742 [4-3] D 11 YDL238C GUD1 guanine deaminase Guanine deaminase; a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures nucleobase-containing small molecule metabolic process hydrolase activity cytoplasm BY4742 [4-3] D 12 YDL239C ADY3 - Protein required for spore wall formation; subunit of the leading edge protein (LEP) complex (Ssp1-Ady3-Don1-Irc10) that forms a ring-like structure at the leading edge of the prospore membrane during meiosis II; mediates assembly of the LEP complex, formation of the ring-like structure via interaction with spindle pole body components and prospore membrane maturation; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole meiotic cell cycle, sporulation, mitochondrion organization, organelle inheritance, cell wall organization or biogenesis molecular_function cytoskeleton, microtubule organizing center, membrane BY4742 [4-3] E 1 YDL240W LRG1 - GTPase-activating protein (GAP); contains Rho1p-specific GAP activity, interacting with activated forms of Rho1p; functions along with Sac7p as a negative regulator of the Pkc1p-mediated cell wall integrity signaling pathway; negative regulator of cell wall 1,3-beta-glucan biosynthesis; required for efficient cell fusion; contains a RhoGAP domain and three Lin-11-Isl1-Mec-3 (LIM) domains carbohydrate metabolic process, conjugation, cell wall organization or biogenesis, signaling enzyme regulator activity mitochondrion, cellular bud, site of polarized growth, cytoplasm BY4742 [4-3] E 2 YDL241W - - Putative protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; YDL241W is not an essential gene biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-3] E 3 YDL242W - - Putative protein of unknown function; conserved across S. cerevisiae strains biological_process molecular_function cellular_component BY4742 [4-3] E 4 YDL243C AAD4 putative aryl-alcohol dehydrogenase Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family other oxidoreductase activity cellular_component BY4742 [4-3] E 5 YDR001C NTH1 alpha,alpha-trehalase NTH1 Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; phosphorylated and activated by Cdc28p at the G1/S phase transition to coordinately regulate carbohydrate metabolism and the cell cycle; inhibited by Dcs1p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication carbohydrate metabolic process, response to chemical, oligosaccharide metabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds cytoplasm BY4742 [4-3] E 6 - cytoplasm cytoplasm cytoplasm - - - - [4-3] E 7 YDR003W RCR2 SSH5 Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication other molecular_function membrane, vacuole, cytoplasm BY4742 [4-3] E 8 YDR004W RAD57 putative DNA-dependent ATPase RAD57 Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p meiotic cell cycle, DNA repair, telomere organization, organelle fission, cellular response to DNA damage stimulus, DNA recombination hydrolase activity, ATPase activity nucleus BY4742 [4-3] E 9 YDR005C MAF1 RNA polymerase III-inhibiting protein MAF1 Highly conserved negative regulator of RNA polymerase III; involved in tRNA processing and stability; inhibits tRNA degradation via rapid tRNA decay (RTD) pathway; binds N-terminal domain of Rpc160p subunit of Pol III to prevent closed-complex formation; regulated by phosphorylation mediated by TORC1, protein kinase A, Sch9p, casein kinase 2; localizes to cytoplasm during vegetative growth and translocates to nucleus and nucleolus under stress conditions chromatin organization, transcription from RNA polymerase III promoter, transcription from RNA polymerase II promoter enzyme binding nucleus, nucleolus, cytoplasm BY4742 [4-3] E 10 YDR006C SOK1 - Protein of unknown function; overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 signaling molecular_function nucleus BY4742 [4-3] E 11 - nucleus nucleus nucleus - - - - [4-3] E 12 YDR008C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps TRP1/YDR007W on opposite strand biological_process molecular_function cellular_component BY4742 [4-3] F 1 YDR009W GAL3 transcriptional regulator GAL3 Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication carbohydrate metabolic process, response to chemical, transcription from RNA polymerase II promoter other nucleus, cytoplasm BY4742 [4-3] F 2 YDR010C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-3] F 3 YDR011W SNQ2 ATP-binding cassette transporter SNQ2 Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species response to chemical, cellular ion homeostasis hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, cytoplasm, plasma membrane BY4742 [4-3] F 4 - mitochondrion, membrane, cytoplasm, plasma membrane mitochondrion, membrane, cytoplasm, plasma membrane mitochondrion, membrane, cytoplasm, plasma membrane - - - - [4-3] F 5 YDR014W RAD61 WPL1 Subunit of a complex that inhibits sister chromatid cohesion; also negatively regulates chromosome condensation; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; binds Smc3p ATPase head of cohesin; related to the human Wapl protein that controls the association of cohesin with chromatin mitotic cell cycle, organelle fission, regulation of organelle organization, chromosome segregation, regulation of cell cycle molecular_function nucleus, chromosome BY4742 [4-3] F 6 YDR015C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A biological_process molecular_function cellular_component BY4742 [4-3] F 7 - cellular_component cellular_component cellular_component - - - - [4-3] F 8 YDR017C KCS1 inositol polyphosphate kinase KCS1 Inositol hexakisphosphate and inositol heptakisphosphate kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response, RNA polymerase I-mediated rRNA transcription and telomere maintenance; inositol hexakisphosphate is also known as IP6; inositol heptakisphosphate is also known as IP7 carbohydrate metabolic process kinase activity, transferase activity cytoplasm BY4742 [4-3] F 9 YDR018C - putative acyltransferase Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication lipid metabolic process transferase activity nucleus, membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-3] F 10 YDR019C GCV1 glycine decarboxylase subunit T|GSD1 T subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4742 [4-3] F 11 YDR020C DAS2 putative uridine kinase DAS2|RRT3 Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases biological_process molecular_function nucleus, cytoplasm BY4742 [4-3] F 12 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-3] G 1 YDR022C ATG31 CIS1 Autophagy-specific protein required for autophagosome formation; forms a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; constitutively phosphorylated, and phosphorylation of residue S174 is required for function; high-copy suppressor of CIK1 deletion mitochondrion organization molecular_function cytoplasm BY4742 [4-3] G 2 - cytoplasm cytoplasm cytoplasm - - - - [4-3] G 3 YDR024W FYV1 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin biological_process molecular_function nucleus, cytoplasm BY4742 [4-3] G 4 YDR025W RPS11A uS17|ribosomal 40S subunit protein S11A|S17|rp41A|YS12|S18A|S11A Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; N-terminally propionylated in vivo; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [4-3] G 5 YDR026C NSI1 YTT1 RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication transcription from RNA polymerase I promoter, chromatin organization, DNA-templated transcription, termination DNA binding nucleus, nucleolus, chromosome, ribosome, cytoplasm BY4742 [4-3] G 6 YDR027C VPS54 TCS3|CGP1|LUV1 Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; potentially phosphorylated by Cdc28p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p meiotic cell cycle, sporulation, Golgi vesicle transport, endosomal transport, cell wall organization or biogenesis molecular_function cytoplasmic vesicle, endomembrane system, mitochondrion, Golgi apparatus, cytoplasm BY4742 [4-3] G 7 YDR028C REG1 protein phosphatase regulator REG1|SRN1|SPP43|PZF240|HEX2 Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; REG1 has a paralog, REG2, that arose from the whole genome duplication carbohydrate metabolic process, response to chemical, chromatin organization, generation of precursor metabolites and energy, response to starvation, transcription from RNA polymerase II promoter enzyme regulator activity cytoplasm BY4742 [4-3] G 8 YDR029W - - Putative protein of unknown function; conserved among S. cerevisiae strains; YDR029W is not an essential gene biological_process molecular_function cellular_component BY4742 [4-3] G 9 YDR030C RAD28 - Protein involved in DNA repair; related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair DNA repair, cellular response to DNA damage stimulus molecular_function nucleus BY4742 [4-3] G 10 YDR031W MIX14 MIC14 Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress generation of precursor metabolites and energy, cellular respiration molecular_function nucleus, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-3] G 11 YDR032C PST2 flavodoxin-like fold family protein Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication biological_process molecular_function membrane, mitochondrion, plasma membrane, cytoplasm BY4742 [4-3] G 12 YDR033W MRH1 - Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; long-lived protein that is asymmetrically retained in the plasma membrane of mother cells; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; null mutation confers sensitivity to acetic acid biological_process molecular_function membrane, endomembrane system, mitochondrion, endoplasmic reticulum, plasma membrane, cytoplasm BY4742 [4-3] H 1 YDR034C LYS14 - Transcriptional activator involved in regulating lysine biosynthesis; involved in the regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [4-3] H 2 YDR035W ARO3 3-deoxy-7-phosphoheptulonate synthase ARO3 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan other transferase activity nucleus, mitochondrion, cytoplasm BY4742 [4-3] H 3 YDR036C EHD3 MRP5 3-hydroxyisobutyryl-CoA hydrolase; member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis biological_process hydrolase activity mitochondrion, cytoplasm BY4742 [4-3] H 4 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [4-3] H 5 - - - - - - - - [4-3] H 6 YDR042C - - Putative protein of unknown function; expression is increased in ssu72-ts69 mutant biological_process molecular_function cellular_component BY4742 [4-3] H 7 YDR043C NRG1 transcriptional regulator NRG1 Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; activated in stochastic pulses of nuclear localization in response to low glucose response to chemical, invasive growth in response to glucose limitation, pseudohyphal growth, transcription from RNA polymerase II promoter, response to osmotic stress nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding, transcription factor binding nucleus BY4742 [4-3] H 8 - nucleus nucleus nucleus - - - - [4-3] H 9 - - - - - - - - [4-3] H 10 YDR046C BAP3 amino acid transporter BAP3|PAP1 Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication transmembrane transport, ion transport, amino acid transport transmembrane transporter activity endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [4-3] H 11 - endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm, plasma membrane - - - - [4-3] H 12 - - - - - - - - [4-4] A 1 YDR049W VMS1 - Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans response to chemical, proteolysis involved in cellular protein catabolic process peptidase activity, hydrolase activity endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm BY4742 [4-4] A 2 - endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm - - - - [4-4] A 3 YDR051C DET1 acid phosphatase DET1 Acid phosphatase; involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel lipid transport phosphatase activity, hydrolase activity nucleus, cytoplasm BY4742 [4-4] A 4 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-4] A 5 - - - - - - - - [4-4] A 6 - - - - - - - - [4-4] A 7 YDR055W PST1 HPF2 Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1; PST1 has a paralog, ECM33, that arose from the whole genome duplication cell wall organization or biogenesis molecular_function cell wall, membrane, vacuole, plasma membrane, cytoplasm BY4742 [4-4] A 8 YDR056C EMC10 - Putative protein of unknown function; subunit of evolutionarily conserved EMC (Endoplasmic Reticulum Membrane Complex) implicated in ERAD (ER-associated degradation) and proper assembly of multi-pass transmembrane (TM) proteins; EMC acts in yeast as an ER-mitochondria tether that interacts with outer membrane protein Tom5p of TOM (Translocase of the Mitochondrial Outer Membrane) complex; YDR056C is not an essential protein biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-4] A 9 YDR057W YOS9 - ER quality-control lectin; integral subunit of the HRD ligase; participates in efficient ER retention of misfolded proteins by recognizing them and delivering them to Hrd1p; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family response to chemical, proteolysis involved in cellular protein catabolic process, signaling other endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [4-4] A 10 YDR059C UBC5 E2 ubiquitin-conjugating protein UBC5 Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication response to heat, protein modification by small protein conjugation or removal transferase activity other BY4742 [4-4] A 11 - other other other - - - - [4-4] A 12 YDR061W - - Protein with similarity to ABC transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance biological_process molecular_function mitochondrion, cytoplasm BY4742 [4-4] B 1 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [4-4] B 2 YDR063W AIM7 GMF1|GMF Protein that interacts with Arp2/3 complex; interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss cytoskeleton organization, regulation of organelle organization, protein complex biogenesis molecular_function nucleus, cell cortex, cytoskeleton, cytoplasm BY4742 [4-4] B 3 - nucleus, cell cortex, cytoskeleton, cytoplasm nucleus, cell cortex, cytoskeleton, cytoplasm nucleus, cell cortex, cytoskeleton, cytoplasm - - - - [4-4] B 4 YDR065W RRG1 - Protein of unknown function; required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies cellular ion homeostasis, mitochondrion organization molecular_function mitochondrion, cytoplasm BY4742 [4-4] B 5 YDR066C RTR2 putative protein-serine/threonine phosphatase Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication biological_process molecular_function nucleus, cytoplasm BY4742 [4-4] B 6 YDR067C OCA6 protein-tyrosine-phosphatase Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT biological_process molecular_function cytoplasm BY4742 [4-4] B 7 YDR068W DOS2 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm BY4742 [4-4] B 8 YDR069C DOA4 ubiquitin-specific protease DOA4|UBP4|SSV7|NPI2|MUT4|DOS1 Ubiquitin hydrolase; deubiquitinates intralumenal vesicle (ILVs) cargo proteins; required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins destined for the vacuole; DOA4 has a paralog, UBP5, that arose from the whole genome duplication DNA replication, ion transport, vesicle organization, proteolysis involved in cellular protein catabolic process, nucleobase-containing compound transport, regulation of DNA metabolic process, endocytosis peptidase activity, hydrolase activity cytoplasmic vesicle, endomembrane system, mitochondrion, cytoplasm BY4742 [4-4] B 9 YDR070C FMP16 - Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress biological_process molecular_function mitochondrion, cytoplasm BY4742 [4-4] B 10 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [4-4] B 11 YDR072C IPT1 MIC2|inositolphosphotransferase|KTI6|SYR4 Inositolphosphotransferase; involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin lipid metabolic process transferase activity membrane BY4742 [4-4] B 12 YDR073W SNF11 - Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions transcription from RNA polymerase II promoter ATPase activity, hydrolase activity nucleus, cytoplasm BY4742 [4-4] C 1 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-4] C 2 YDR075W PPH3 phosphoprotein phosphatase PP4 catalytic subunit PPH3 Catalytic subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form active complex, Psy4p may provide substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes meiotic cell cycle, response to chemical, organelle fission, protein dephosphorylation, DNA recombination, DNA repair, regulation of organelle organization, cellular response to DNA damage stimulus, regulation of DNA metabolic process, regulation of cell cycle, signaling phosphatase activity, hydrolase activity nucleus, chromosome, cytoplasm BY4742 [4-4] C 3 YDR076W RAD55 putative DNA-dependent ATPase RAD55 Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p meiotic cell cycle, DNA repair, organelle fission, DNA recombination, cellular response to DNA damage stimulus ATPase activity, hydrolase activity nucleus BY4742 [4-4] C 4 YDR077W SED1 - Major stress-induced structural GPI-cell wall glycoprotein; associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites; SED1 has a paralog, SPI1, that arose from the whole genome duplication mitochondrion organization, cell wall organization or biogenesis structural molecule activity cell wall, ribosome, mitochondrion, cytoplasm BY4742 [4-4] C 5 YDR078C SHU2 - Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Csm2, Shu1, and promotes error-free DNA repair, Shu complex mediates inhibition of Srs2p function; promotes formation of Rad51p filaments; important for both mitotic and meiotic homologous recombination, and contains a conserved SWIM domain that is necessary for both DNA repair, DNA recombination, cellular response to DNA damage stimulus molecular_function chromosome BY4742 [4-4] C 6 YDR079W PET100 - Chaperone that facilitates the assembly of cytochrome c oxidase; integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme mitochondrion organization, protein complex biogenesis unfolded protein binding membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-4] C 7 YDR080W VPS41 VPL20|VAM2|SVL2|FET2|CVT8 Subunit of the HOPS endocytic tethering complex; vacuole membrane protein that functions as a Rab GTPase effector, interacting specifically with the GTP-bound conformation of Ypt7p, facilitating tethering, docking and promoting membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; Yck3p-mediated phosphorylation regulates the organization of vacuolar fusion sites vacuole organization, regulation of transport, organelle fusion, protein maturation, protein targeting, regulation of organelle organization, vesicle organization, protein complex biogenesis, membrane fusion enzyme binding, ion binding, lipid binding vacuole, cytoplasmic vesicle, endomembrane system, membrane, cytoplasm BY4742 [4-4] C 8 - vacuole, cytoplasmic vesicle, endomembrane system, membrane, cytoplasm vacuole, cytoplasmic vesicle, endomembrane system, membrane, cytoplasm vacuole, cytoplasmic vesicle, endomembrane system, membrane, cytoplasm - - - - [4-4] C 9 - - - - - - - - [4-4] C 10 YDR083W RRP8 25S rRNA (adenine645-N1)-methyltransferase Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 rRNA processing, ribosomal large subunit biogenesis, RNA modification transferase activity, methyltransferase activity nucleus, nucleolus BY4742 [4-4] C 11 YDR084C TVP23 - Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern other molecular_function membrane, endomembrane system, cytoplasm, Golgi apparatus BY4742 [4-4] C 12 YDR085C AFR1 - Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication response to chemical, cell morphogenesis, conjugation, signaling signal transducer activity other BY4742 [4-4] D 1 - other other other - - - - [4-4] D 2 - - - - - - - - [4-4] D 3 - - - - - - - - [4-4] D 4 YDR089W VTC5 - Novel subunit of the vacuolar transporter chaperone complex; vacuolar transmembrane protein that regulates biosynthesis of polyphosphate; deletion reduces and overexpression increases polyP accumulation; SPX domain (Syg1, Pho81, Xpr1)-containing protein involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress cellular ion homeostasis molecular_function membrane, vacuole, cytoplasm BY4742 [4-4] D 5 YDR090C - - Putative protein of unknown function biological_process molecular_function membrane BY4742 [4-4] D 6 - membrane membrane membrane - - - - [4-4] D 7 YDR092W UBC13 E2 ubiquitin-conjugating protein UBC13 E2 ubiquitin-conjugating enzyme; involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus DNA repair, protein modification by small protein conjugation or removal, cellular response to DNA damage stimulus, protein complex biogenesis transferase activity nucleus, cytoplasm BY4742 [4-4] D 8 YDR093W DNF2 aminophospholipid-translocating P4-type ATPase DNF2 Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF2 has a paralog, DNF1, that arose from the whole genome duplication response to chemical, ion transport, lipid transport, conjugation, endocytosis hydrolase activity, ATPase activity endomembrane system, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [4-4] D 9 YDR094W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2/YDR093W biological_process molecular_function cellular_component BY4742 [4-4] D 10 YDR095C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-4] D 11 YDR096W GIS1 histone demethylase GIS1 Histone demethylase and transcription factor; regulates genes during nutrient limitation; activity modulated by proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminus that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; relocalizes to the cytosol in response to hypoxia; GIS1 has a paralog, RPH1, that arose from the whole genome duplication chromatin organization, histone modification, lipid metabolic process, response to starvation, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, oxidoreductase activity, DNA binding nucleus, mitochondrion, cytoplasm BY4742 [4-4] D 12 YDR097C MSH6 PMS6|mismatch repair ATPase MSH6|PMS3 Protein required for mismatch repair in mitosis and meiosis; forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p meiotic cell cycle, DNA repair, organelle fission, DNA replication, cellular response to DNA damage stimulus, regulation of DNA metabolic process hydrolase activity, DNA binding, ion binding, ATPase activity nucleus, cytoplasm BY4742 [4-4] E 1 YDR098C GRX3 monothiol glutaredoxin GRX3 Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; with Grx4p, binds to Aft1p in iron-replete conditions, promoting its dissociation from promoters; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36; GRX3 has a paralog, GRX4, that arose from the whole genome duplication response to chemical, chromatin organization, cytoskeleton organization, response to oxidative stress, histone modification, regulation of protein modification process, cellular ion homeostasis oxidoreductase activity, transcription factor binding nucleus, cytoplasm BY4742 [4-4] E 2 YDR099W BMH2 14-3-3 family protein BMH2|SCD3 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; abundance relative to Bmh1p increases during sporulation mitotic cell cycle, meiotic cell cycle, DNA replication, generation of precursor metabolites and energy, cell wall organization or biogenesis, carbohydrate metabolic process, sporulation, cellular response to DNA damage stimulus, regulation of protein modification process, protein modification by small protein conjugation or removal, pseudohyphal growth, signaling, regulation of cell cycle ion binding, DNA binding nucleus, plasma membrane, membrane, cytoplasm BY4742 [4-4] E 3 YDR100W TVP15 - Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p other molecular_function membrane, cytoplasmic vesicle, endomembrane system, cytoplasm, Golgi apparatus BY4742 [4-4] E 4 YDR101C ARX1 putative hydrolase Nuclear export factor for the ribosomal pre-60S subunit; shuttling factor which directly binds FG rich nucleoporins and facilities translocation through the nuclear pore complex; interacts directly with Alb1p; responsible for Tif6p recycling defects in the absence of Rei1; associated with the ribosomal export complex ribosomal subunit export from nucleus, nuclear transport molecular_function nucleus, nucleolus, ribosome, cytoplasm BY4742 [4-4] E 5 YDR102C - - Putative protein of unknown function; conserved among S. cerevisiae strains; YDR102C is not an essential gene; homozygous diploid deletion strain exhibits high budding index biological_process molecular_function cellular_component BY4742 [4-4] E 6 YDR103W STE5 NUL3|HMD3 Pheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation response to chemical, protein phosphorylation, invasive growth in response to glucose limitation, transposition, regulation of protein modification process, conjugation, signaling lipid binding, enzyme binding, structural molecule activity, ion binding nucleus, membrane, site of polarized growth, cytoplasm, plasma membrane BY4742 [4-4] E 7 YDR104C SPO71 - Meiosis-specific protein required for prospore membrane morphogenesis; localizes to the prospore membrane (PSM) during sporulation; required for PSM elongation and closure; genetically antagonistic to SPO1; recruits Vps13p to the PSM during sporulation; interacts and functions cooperatively with Spo73p; mutants have defects in the PSM, aberrant spore wall formation and reduced PtdIns-phosphate pools in the PSM; contains three PH-like domains meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function membrane, plasma membrane BY4742 [4-4] E 8 YDR105C TMS1 - Vacuolar membrane protein of unknown function; is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [4-4] E 9 YDR107C TMN2 - Protein with a role in cellular adhesion and filamentous growth; similar to Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments; TMN2 has a paralog, EMP70, that arose from the whole genome duplication cellular ion homeostasis, invasive growth in response to glucose limitation, pseudohyphal growth molecular_function membrane, vacuole, cytoplasm BY4742 [4-4] E 10 YDR108W TRS85 GSG1|MUM1 Component of transport protein particle (TRAPP) complex III; TRAPPIII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating endosome-Golgi traffic and required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role vacuole organization, meiotic cell cycle, protein targeting, organelle fission, organelle assembly, vesicle organization, Golgi vesicle transport molecular_function cytoplasmic vesicle, endomembrane system, Golgi apparatus, cytoplasm BY4742 [4-4] E 11 YDR109C - putative phosphotransferase Putative kinase biological_process molecular_function cellular_component BY4742 [4-4] E 12 YDR110W FOB1 replication fork barrier binding protein FOB1|HRM1 Nucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to replication fork barrier (RFB), and rDNA repeat segregation; related to retroviral integrases chromatin organization, DNA replication, chromosome segregation, DNA recombination, regulation of DNA metabolic process, nucleus organization DNA binding nucleus, chromosome, nucleolus BY4742 [4-4] F 1 YDR111C ALT2 alanine transaminase ALT2 Catalytically inactive alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p; ALT2 has a paralog, ALT1, that arose from the whole genome duplication biological_process transferase activity nucleus, cytoplasm BY4742 [4-4] F 2 YDR112W IRC2 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ALT2/YDR111C; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function cellular_component BY4742 [4-4] F 3 - cellular_component cellular_component cellular_component - - - - [4-4] F 4 YDR114C - - Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium biological_process molecular_function cellular_component BY4742 [4-4] F 5 YDR115W MRX14 putative mitochondrial 54S ribosomal protein Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress mitochondrial translation, mitochondrion organization structural constituent of ribosome, structural molecule activity nucleus, ribosome, mitochondrion, cytoplasm BY4742 [4-4] F 6 YDR116C MRPL1 mitochondrial 54S ribosomal protein MRPL1 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [4-4] F 7 YDR117C TMA64 RBF64 Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity rRNA processing RNA binding ribosome, cytoplasm BY4742 [4-4] F 8 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [4-4] F 9 YDR119W VBA4 - Protein of unknown function; proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [4-4] F 10 YDR120C TRM1 tRNA (guanine26-N2)-dimethyltransferase tRNA methyltransferase; two forms of protein are made by alternative translation starts; localizes to both nucleus and mitochondrion to produce modified base N2,N2-dimethylguanosine in tRNAs in both compartments; nuclear Trm1p is evenly distributed around inner nuclear membrane in WT, but mislocalizes as puncta near ER-nucleus junctions in spindle pole body (SPB) mutants; both Trm1p inner nuclear membrane targeting and maintenance depend upon SPB tRNA processing, RNA modification transferase activity, methyltransferase activity nucleus, endomembrane system, mitochondrion, membrane, cytoplasm BY4742 [4-4] F 11 YDR121W DPB4 DNA polymerase epsilon noncatalytic subunit Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization DNA repair, chromatin organization, DNA replication, cellular response to DNA damage stimulus chromatin binding, transferase activity, nucleotidyltransferase activity, DNA binding nucleus, chromosome BY4742 [4-4] F 12 YDR122W KIN1 serine/threonine protein kinase KIN1 Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; KIN1 has a paralog, KIN2, that arose from the whole genome duplication protein phosphorylation, exocytosis kinase activity, transferase activity membrane, plasma membrane BY4742 [4-4] G 1 YDR123C INO2 SCS1|DIE1 Transcription factor; component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion; involved in diauxic shift lipid metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity nucleus BY4742 [4-4] G 2 YDR124W - - Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor biological_process molecular_function cellular_component BY4742 [4-4] G 3 YDR125C ECM18 alpha/beta hydrolase family protein Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication biological_process molecular_function mitochondrion, cytoplasm BY4742 [4-4] G 4 YDR126W SWF1 palmitoyltransferase SWF1|PSL10 Palmitoyltransferase that acts on transmembrane proteins; including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion vacuole organization, meiotic cell cycle, protein acylation, cytoskeleton organization, regulation of transport, organelle fusion, cell wall organization or biogenesis, protein lipidation, sporulation, membrane fusion, exocytosis transferase activity cell cortex, cytoskeleton, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [4-4] G 5 YDR127W ARO1 pentafunctional protein ARO1p Pentafunctional arom protein; catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids other lyase activity, kinase activity, transferase activity, oxidoreductase activity cytoplasm BY4742 [4-4] G 6 YDR128W MTC5 SEA3 Subunit of SEACAT, a subcomplex of the SEA complex; Mtc1p, along with Rtc1p and Sea4p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; relative distribution to the vacuolar membrane decreases upon DNA replication stress signaling molecular_function membrane, vacuole, cytoplasm BY4742 [4-4] G 7 YDR129C SAC6 fimbrin|ABP67 Fimbrin, actin-bundling protein; cooperates with Scp1p in organization and maintenance of the actin cytoskeleton; phosphorylated by Cdc28p/Clb2p in metaphase on T103, to regulate conformation, and modulate actin filament binding affinity and actin cable dynamics; relocalizes from the plasma membrane to the cytoplasm upon DNA replication stress; human homologs PLS3 and LCP1 implicated in spinocerebellar ataxia type 2 (SCA2) can each complement yeast null mutant cytoskeleton organization protein binding, bridging, cytoskeletal protein binding cell cortex, cytoskeleton, cellular bud, membrane, site of polarized growth, cytoplasm, plasma membrane BY4742 [4-4] G 8 YDR130C FIN1 - Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, chromosome segregation, regulation of cell cycle cytoskeletal protein binding, structural molecule activity nucleus, cytoskeleton, microtubule organizing center, chromosome, cytoplasm BY4742 [4-4] G 9 YDR131C - - F-box protein subunit of SCF ubiquitin ligase complex; substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex proteolysis involved in cellular protein catabolic process protein binding, bridging, transferase activity other BY4742 [4-4] G 10 YDR132C - - Protein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication biological_process molecular_function nucleus, mitochondrion, cytoplasm BY4742 [4-4] G 11 YDR133C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps YDR134C biological_process molecular_function cellular_component BY4742 [4-4] G 12 YDR134C cellular_component cellular_component cellular_component - - - BY4742 [4-4] H 1 YDR135C YCF1 ATP-binding cassette glutathione S-conjugate transporter YCF1 Vacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR vacuole organization, response to chemical, transmembrane transport, ion transport, organelle fusion, membrane fusion hydrolase activity, transmembrane transporter activity, ATPase activity vacuole, membrane, cytoplasm BY4742 [4-4] H 2 YDR136C VPS61 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect protein targeting molecular_function cellular_component BY4742 [4-4] H 3 YDR137W RGP1 - Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p); this complex catalyzes nucleotide exchange on Ypt6p endosomal transport guanyl-nucleotide exchange factor activity membrane, endomembrane system, cytoplasm, Golgi apparatus BY4742 [4-4] H 4 YDR138W HPR1 TRF1 Subunit of THO/TREX complexes; this complex couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p transcription from RNA polymerase I promoter, DNA repair, cellular response to DNA damage stimulus, nucleobase-containing compound transport, DNA recombination, mRNA processing, transcription from RNA polymerase II promoter, nuclear transport, DNA-templated transcription, elongation structural molecule activity nucleus, chromosome BY4742 [4-4] H 5 YDR139C RUB1 NEDD8 family protein RUB1 Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) protein modification by small protein conjugation or removal other cytoplasm BY4742 [4-4] H 6 YDR140W MTQ2 S-adenosylmethionine-dependent methyltransferase S-adenosylmethionine-dependent methyltransferase; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC; member of the seven beta-strand family regulation of translation, protein alkylation transferase activity, methyltransferase activity nucleus, cytoplasm BY4742 [4-4] H 7 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-4] H 8 YDR142C PEX7 PEB1|PAS7 Peroxisomal signal receptor for peroxisomal matrix proteins; recognizes the N-terminal nonapeptide signal (PTS2); WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) protein targeting, transmembrane transport, peroxisome organization other peroxisome, cytoplasm BY4742 [4-4] H 9 YDR143C SAN1 ubiquitin-protein ligase SAN1 Ubiquitin-protein ligase; involved in proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition; prefers a window of exposed hydrophobicity that causes a particular level of protein insolubility, suggesting that San1p evolved to target highly aggregation-prone proteins response to chemical, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process transferase activity nucleus, cytoplasm BY4742 [4-4] H 10 YDR144C MKC7 aspartyl protease|YPS2 GPI-anchored aspartyl protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p; MKC7 has a paralog, YPS1, that arose from the whole genome duplication cell wall organization or biogenesis, signaling peptidase activity, hydrolase activity cell wall, vacuole, cytoplasm BY4742 [4-4] H 11 - cell wall, vacuole, cytoplasm cell wall, vacuole, cytoplasm cell wall, vacuole, cytoplasm - - - - [4-4] H 12 YDR146C SWI5 DNA-binding transcription factor SWI5 Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication mitotic cell cycle, organelle fission nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding nucleus, cytoplasm BY4742 [4-5] A 1 YDR147W EKI1 bifunctional choline kinase/ethanolamine kinase EKI1 Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication lipid metabolic process kinase activity, transferase activity cytoplasm BY4742 [4-5] A 2 YDR148C KGD2 alpha-ketoglutarate dehydrogenase KGD2 Dihydrolipoyl transsuccinylase; component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated mitochondrion organization, generation of precursor metabolites and energy, cellular respiration transferase activity mitochondrion, cytoplasm BY4742 [4-5] A 3 YDR149C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1/YDR150W; null mutation blocks anaerobic growth biological_process molecular_function cellular_component BY4742 [4-5] A 4 YDR150W NUM1 PAC12 Protein required for nuclear migration; component of the mitochondria-ER-cortex-ancor (MECA); required for the association of mitochondria with the cell cortex and for accurate distribution of mitochondrial network; interacts with Mdm36p to link the ER and mitochondria at the cortex; localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex organelle fission, mitochondrion organization, cytoskeleton organization, organelle inheritance cytoskeletal protein binding cell cortex, endomembrane system, mitochondrion, cellular bud, site of polarized growth, endoplasmic reticulum, cytoplasm BY4742 [4-5] A 5 YDR151C CTH1 putative mRNA-binding protein CTH1 Member of the CCCH zinc finger family; similar to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis; CTH1 has a paralog, TIS11, that arose from the whole genome duplication RNA catabolic process, cellular ion homeostasis RNA binding, mRNA binding nucleus BY4742 [4-5] A 6 YDR152W GIR2 - Highly-acidic RWD domain-containing cytoplasmic protein; forms a highly conserved complex with Rbg2p that is responsible for efficient cell growth under amino acid starvation and binds translational activator Gcn1p in dose-dependent manner according to stress level; associates with translating ribosomes; intrinsically unstructured protein whose stability is enhanced upon binding Rbg2p cytoplasmic translation, response to starvation other cytoplasm BY4742 [4-5] A 7 YDR153C ENT5 - Protein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Golgi vesicle transport, endosomal transport lipid binding, ion binding cytoplasmic vesicle, endomembrane system, membrane, cytoplasm BY4742 [4-5] A 8 YDR154C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component BY4742 [4-5] A 9 YDR155C CPR1 peptidylprolyl isomerase CPR1|CPH1|CYP1 Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; N-terminally propionylated in vivo; protein abundance increases in response to DNA replication stress meiotic cell cycle, organelle fission, chromatin organization, sporulation, regulation of organelle organization, histone modification, regulation of cell cycle RNA binding, isomerase activity, mRNA binding nucleus, chromosome, mitochondrion, mitochondrial envelope, cytoplasm BY4742 [4-5] A 10 YDR156W RPA14 DNA-directed RNA polymerase I subunit RPA14|A14 RNA polymerase I subunit A14 transcription from RNA polymerase I promoter transferase activity, nucleotidyltransferase activity nucleus, nucleolus BY4742 [4-5] A 11 YDR157W - - Putative protein of unknown function; conserved across S. cerevisiae strains biological_process molecular_function cellular_component BY4742 [4-5] A 12 YDR158W HOM2 aspartate-semialdehyde dehydrogenase|THR2 Aspartic beta semi-aldehyde dehydrogenase; catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis cellular amino acid metabolic process oxidoreductase activity nucleus, membrane, plasma membrane, cytoplasm BY4742 [4-5] B 1 YDR159W SAC3 LEP1 mRNA export factor; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) mitotic cell cycle, DNA repair, organelle fission, cellular response to DNA damage stimulus, nucleobase-containing compound transport, ribosomal small subunit biogenesis, RNA catabolic process, mRNA processing, nuclear transport other nucleus, endomembrane system BY4742 [4-5] B 2 - nucleus, endomembrane system nucleus, endomembrane system nucleus, endomembrane system - - - - [4-5] B 3 YDR161W ACL4 - Specific assembly chaperone for ribosomal protein Rpl4p; binds to an evolutionarily conserved surface extension of nascent Rpl4p and chaperones Rpl4p until its assembly into the pre-ribosome; transcriptionally co-regulated with rRNA and ribosome biosynthesis genes response to chemical, proteolysis involved in cellular protein catabolic process molecular_function nucleus, cytoplasm BY4742 [4-5] B 4 YDR162C NBP2 - Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) response to heat, protein phosphorylation, regulation of protein modification process, cell wall organization or biogenesis, signaling, response to osmotic stress protein binding, bridging nucleus, cytoplasm BY4742 [4-5] B 5 YDR163W CWC15 U2-type spliceosomal complex subunit CWC15 Non-essential protein involved in pre-mRNA splicing; component of a complex containing Cef1p; has similarity to S. pombe Cwf15p RNA splicing, mRNA processing molecular_function nucleus BY4742 [4-5] B 6 - nucleus nucleus nucleus - - - - [4-5] B 7 YDR165W TRM82 - Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p; relocalizes to the cytosol in response to hypoxia; mutation in human ortholog WDR4 causes microcephalic primordial dwarfism tRNA processing, RNA modification transferase activity, methyltransferase activity nucleus, cytoplasm BY4742 [4-5] B 8 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-5] B 9 - - - - - - - - [4-5] B 10 - - - - - - - - [4-5] B 11 YDR169C STB3 - Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress response to chemical, transcription from RNA polymerase II promoter DNA binding nucleus, cytoplasm BY4742 [4-5] B 12 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-5] C 1 YDR171W HSP42 heat shock protein HSP42 Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress cytoskeleton organization unfolded protein binding cytoskeleton, cytoplasm BY4742 [4-5] C 2 - cytoskeleton, cytoplasm cytoskeleton, cytoplasm cytoskeleton, cytoplasm - - - - [4-5] C 3 YDR173C ARG82 inositol polyphosphate multikinase|ARGRIII|IPK2|ARGR3 Inositol polyphosphate multikinase (IPMK); sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes lipid metabolic process, transcription from RNA polymerase II promoter, cellular amino acid metabolic process protein binding, bridging, kinase activity, transferase activity nucleus BY4742 [4-5] C 4 - nucleus nucleus nucleus - - - - [4-5] C 5 YDR175C RSM24 mitochondrial 37S ribosomal protein RSM24 Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [4-5] C 6 YDR176W NGG1 histone acetyltransferase NGG1|SWI7|ADA3 Subunit of chromatin modifying histone acetyltransferase complexes; member of the ADA complex, the SAGA complex, and the SLIK complex; transcriptional regulator involved in glucose repression of Gal4p-regulated genes peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity nucleus BY4742 [4-5] C 7 - nucleus nucleus nucleus - - - - [4-5] C 8 YDR178W SDH4 succinate dehydrogenase membrane anchor subunit SDH4|ACN18 Membrane anchor subunit of succinate dehydrogenase (SDH); involved in coupling the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; has similarity to human SDH subunit D (SDHD), which is implicated in paraganglioma generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [4-5] C 9 YDR179C CSN9 - Subunit of the Cop9 signalosome; Cop9 signalosome is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling response to chemical, protein modification by small protein conjugation or removal, conjugation, signaling molecular_function nucleus BY4742 [4-5] C 10 YDR179W-A NVJ3 - Protein with a potential role in tethering ER and vacuoles; localizes to nucleus-vacuole junctions in an Mdm1p-dependent manner; contains a lipid-binding PXA domain other ion binding, lipid binding other BY4742 [4-5] C 11 - other other other - - - - [4-5] C 12 YDR181C SAS4 - Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p chromatin organization transferase activity nucleus, chromosome BY4742 [4-5] D 1 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [4-5] D 2 YDR183W PLP1 - Protein that interacts with CCT (chaperonin containing TCP-1) complex; has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators response to chemical, protein folding, transcription from RNA polymerase II promoter other cellular_component BY4742 [4-5] D 3 YDR184C ATC1 LIC4 Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress mitotic cell cycle, cellular ion homeostasis, cell budding, cytokinesis molecular_function nucleus, cytoplasm BY4742 [4-5] D 4 YDR185C UPS3 GEP2 Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations; UPS3 has a paralog, UPS2, that arose from the whole genome duplication mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-5] D 5 YDR186C SND1 - Protein involved in SRP-independent targeting of substrates to the ER; component of an alternative ER targeting pathway that has partial functional redundancy with the GET pathway; preference for substrates with downstream transmembrane domains; interacts with Env10p/Snd2p and Pho88p/Snd3p; can compensate for loss of SRP; may interact with ribosomes, based on co-purification experiments; GFP-fusion protein localizes to the cytoplasm biological_process molecular_function ribosome, cytoplasm BY4742 [4-5] D 6 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [4-5] D 7 - - - - - - - - [4-5] D 8 - - - - - - - - [4-5] D 9 - - - - - - - - [4-5] D 10 YDR191W HST4 NAD-dependent histone deacetylase HST4 NAD(+)-dependent protein deacetylase; deacetylation targets are primarily mitochondrial proteins; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism; accumulates in mitochondria in response to biotin starvation and may link biotin metabolism with energy homeostasis; member of the Sir2 family and may be the functional equivalent of human SIRT3 chromatin organization, monocarboxylic acid metabolic process, histone modification, lipid metabolic process, response to starvation hydrolase activity nucleus, mitochondrion, cytoplasm BY4742 [4-5] D 11 YDR192C NUP42 FG-nucleoporin NUP42|UIP1|RIP1 FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p response to heat, protein targeting, nucleobase-containing compound transport, nuclear transport, response to osmotic stress structural molecule activity nucleus, membrane, endomembrane system BY4742 [4-5] D 12 YDR193W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane BY4742 [4-5] E 1 YDR194C MSS116 ATP-dependent RNA helicase Mitochondrial transcription elongation factor; DEAD-box protein; required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing; promotes RNA folding by stabilizing an early assembly intermediate RNA splicing, DNA-templated transcription, elongation RNA binding, hydrolase activity, ATPase activity, helicase activity, mRNA binding mitochondrion, cytoplasm BY4742 [4-5] E 2 YDR195W REF2 RNA-processing protein REF2 RNA-binding protein; involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes; putative regulatory subunit of type 1 protein phosphatase Glc7p, required for actomyosin ring formation, and for timely dephosphorylation and release of Bnr1p from the division site; relocalizes to the cytosol in response to hypoxia mitotic cell cycle, snoRNA processing, protein dephosphorylation, cytoskeleton organization, regulation of organelle organization, cytokinesis, DNA-templated transcription, termination, mRNA processing, transcription from RNA polymerase II promoter, regulation of cell cycle chromatin binding, RNA binding nucleus, cytoplasm BY4742 [4-5] E 3 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-5] E 4 YDR197W CBS2 CBP7 Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other ribosome, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [4-5] E 5 YDR198C RKM2 protein-lysine N-methyltransferase Ribosomal protein lysine methyltransferase; responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp peptidyl-amino acid modification, protein alkylation transferase activity, methyltransferase activity cellular_component BY4742 [4-5] E 6 YDR199W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64/YDR200C; computationally predicted to have thiol-disulfide oxidoreductase activity biological_process molecular_function cellular_component BY4742 [4-5] E 7 YDR200C VPS64 FAR9 Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance; VPS64 has a paralog, FAR10, that arose from the whole genome duplication response to chemical, protein targeting, conjugation, signaling molecular_function endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [4-5] E 8 - endomembrane system, membrane, endoplasmic reticulum, cytoplasm endomembrane system, membrane, endoplasmic reticulum, cytoplasm endomembrane system, membrane, endoplasmic reticulum, cytoplasm - - - - [4-5] E 9 - - - - - - - - [4-5] E 10 YDR203W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF RAV2/YDR202C biological_process molecular_function cellular_component BY4742 [4-5] E 11 YDR204W COQ4 ubiquinone biosynthesis protein COQ4 Protein with a role in ubiquinone (Coenzyme Q) biosynthesis; possibly functioning in stabilization of Coq7p; located on matrix face of mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex; human homolog COQ4 can complement yeast coq4 null mutant cofactor metabolic process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-5] E 12 - membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm - - - - [4-5] F 1 YDR206W EBS1 - Protein involved in translation inhibition and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors; EBS1 has a paralog, EST1, that arose from the whole genome duplication RNA catabolic process, regulation of translation, DNA recombination molecular_function cytoplasm BY4742 [4-5] F 2 YDR207C UME6 DNA-binding transcriptional regulator UME6|RIM16|NIM2|CAR80 Rpd3L histone deacetylase complex subunit; key transcriptional regulator of early meiotic genes; involved in chromatin remodeling and transcriptional repression via DNA looping; binds URS1 upstream regulatory sequence, represses transcription by recruiting conserved histone deacetylase Rpd3p (through co-repressor Sin3p) and chromatin-remodeling factor Isw2p; couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms compl meiotic cell cycle, response to chemical, organelle fission, chromatin organization, lipid metabolic process, transcription from RNA polymerase II promoter, carbohydrate metabolic process, regulation of organelle organization, pseudohyphal growth, regulation of cell cycle nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding, transcription factor binding nucleus, chromosome BY4742 [4-5] F 3 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [4-5] F 4 YDR209C - - Putative protein of unknown function; conserved among S. cerevisiae strains; YDR209C is not an essential gene; partially overlaps uncharacterized gene YDR210W biological_process molecular_function cellular_component BY4742 [4-5] F 5 YDR210W - - Predicted tail-anchored plasma membrane protein; contains a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function cellular_component BY4742 [4-5] F 6 - cellular_component cellular_component cellular_component - - - - [4-5] F 7 - - - - - - - - [4-5] F 8 YDR213W UPC2 MOX4 Sterol regulatory element binding protein; induces sterol biosynthetic genes, upon sterol depletion; acts as a sterol sensor, binding ergosterol in sterol rich conditions; relocates from intracellular membranes to perinuclear foci upon sterol depletion; redundant activator of filamentation with ECM22, up-regulating the expression of filamentous growth genes; contains a Zn[2]-Cys[6] binuclear cluster; UPC2 has a paralog, ECM22, that arose from the whole genome duplication response to chemical, regulation of transport, lipid metabolic process, lipid transport, response to starvation, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, membrane, cytoplasm BY4742 [4-5] F 9 YDR214W AHA1 - Co-chaperone that binds Hsp82p and activates its ATPase activity; plays a role in determining prion variants; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress response to heat, protein folding enzyme regulator activity cytoplasm BY4742 [4-5] F 10 YDR215C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein biological_process molecular_function cellular_component BY4742 [4-5] F 11 YDR216W ADR1 DNA-binding transcription factor ADR1 Carbon source-responsive zinc-finger transcription factor; required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization response to chemical, chromatin organization, regulation of organelle organization, peroxisome organization, monocarboxylic acid metabolic process, lipid metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding, transcription factor binding nucleus, cytoplasm BY4742 [4-5] F 12 YDR217C RAD9 chromatin-binding protein RAD9 DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p mitotic cell cycle, DNA repair, cellular response to DNA damage stimulus, transcription from RNA polymerase II promoter, regulation of cell cycle histone binding, enzyme regulator activity, DNA binding nucleus, chromosome BY4742 [4-5] G 1 YDR218C SPR28 septin SPR28 Sporulation-specific homolog of the CDC3/10/11/12 family of genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation; the yeast CDC3/10/11/12 family is a family of bud neck microfilament genes meiotic cell cycle, sporulation structural molecule activity cell cortex, cytoskeleton, cell wall, membrane, cytoplasm BY4742 [4-5] G 2 YDR219C MFB1 - Mitochondria-associated F-box protein; involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding mitochondrion organization, proteolysis involved in cellular protein catabolic process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-5] G 3 YDR220C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component BY4742 [4-5] G 4 YDR221W GTB1 - Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress protein glycosylation, carbohydrate metabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-5] G 5 YDR222W - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YDR222W has a paralog, YLR225C, that arose from the whole genome duplication biological_process molecular_function cytoplasm BY4742 [4-5] G 6 YDR223W CRF1 - Transcriptional corepressor; involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain; CRF1 has a paralog, IFH1, that arose from the whole genome duplication transcription from RNA polymerase II promoter transcription factor activity, protein binding nucleus, chromosome, cytoplasm BY4742 [4-5] G 7 - nucleus, chromosome, cytoplasm nucleus, chromosome, cytoplasm nucleus, chromosome, cytoplasm - - - - [4-5] G 8 YDR225W HTA1 histone H2A|SPT11|H2A1 Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; N-terminally propionylated in vivo DNA repair, chromatin organization, cellular response to DNA damage stimulus, transcription from RNA polymerase II promoter DNA binding nucleus, chromosome BY4742 [4-5] G 9 YDR226W ADK1 adenylate kinase ADK1|AKY2|AKY1 Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress; mutations affecting Adk1p catalytic activity deregulate expression of phosphate utilization genes PHO5 and PHO84; human homolog AK1 can complement yeast adk1 mutant DNA replication, nucleobase-containing small molecule metabolic process kinase activity, transferase activity mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-5] G 10 YDR227W SIR4 chromatin-silencing protein SIR4|UTH2|STE9|ASD1 SIR protein involved in assembly of silent chromatin domains; silent information regulator (SIR) along with SIR2 and SIR3; involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; some alleles of SIR4 prolong lifespan; required for telomere hypercluster formation in quiescent yeast cells chromatin organization chromatin binding, DNA binding nucleus, chromosome BY4742 [4-5] G 11 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [4-5] G 12 YDR229W IVY1 - Phospholipid-binding protein that interacts with both Ypt7p and Vps33p; may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase vacuole organization, organelle fusion, membrane fusion lipid binding, ion binding vacuole, membrane, cytoplasm BY4742 [4-5] H 1 YDR230W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20/YDR231C biological_process molecular_function cellular_component BY4742 [4-5] H 2 YDR231C COX20 - Mitochondrial inner membrane protein; required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase mitochondrion organization, protein maturation, generation of precursor metabolites and energy, protein complex biogenesis, cellular respiration unfolded protein binding mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [4-5] H 3 - mitochondrion, membrane, mitochondrial envelope, cytoplasm mitochondrion, membrane, mitochondrial envelope, cytoplasm mitochondrion, membrane, mitochondrial envelope, cytoplasm - - - - [4-5] H 4 YDR233C RTN1 - Reticulon protein; involved in nuclear pore assembly and maintenance of tubular ER morphology; promotes membrane curvature; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; increases tubular ER when overexpressed; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; member of the RTNLA subfamily peroxisome organization, protein complex biogenesis, organelle inheritance, nucleus organization molecular_function cell cortex, endomembrane system, mitochondrion, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4742 [4-5] H 5 YDR234W LYS4 homoaconitate hydratase LYS4|LYS3 Homoaconitase; catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway cellular amino acid metabolic process lyase activity mitochondrion, cytoplasm BY4742 [4-5] H 6 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [4-5] H 7 - - - - - - - - [4-5] H 8 YDR237W MRPL7 mitochondrial 54S ribosomal protein YmL7/YmL5|YmL7|YmL5 Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [4-5] H 9 - ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm - - - - [4-5] H 10 YDR239C - - Protein of unknown function; may interact with ribosomes, based on co-purification experiments biological_process molecular_function ribosome, cytoplasm BY4742 [4-5] H 11 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [4-5] H 12 YDR241W BUD26 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay mitotic cell cycle, cytokinesis molecular_function cellular_component BY4742 [4-6] A 1 YDR242W AMD2 putative amidase Putative amidase biological_process hydrolase activity cellular_component BY4742 [4-6] A 2 - cellular_component cellular_component cellular_component - - - - [4-6] A 3 YDR244W PEX5 PAS10 Peroxisomal membrane signal receptor for peroxisomal matrix proteins; receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins; required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions protein targeting, transmembrane transport, peroxisome organization protein binding, bridging peroxisome, membrane, cytoplasm BY4742 [4-6] A 4 YDR245W MNN10 alpha-1,6-mannosyltransferase|REC41|SLC2|BED1 Subunit of a Golgi mannosyltransferase complex; complex mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family; other members of the complex are Anp1p, Mnn9p, Mnn11p, and Hoc1p mitotic cell cycle, cytokinesis, protein glycosylation, cell wall organization or biogenesis transferase activity, transferring glycosyl groups, transferase activity endomembrane system, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4742 [4-6] A 5 - endomembrane system, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm endomembrane system, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm endomembrane system, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm - - - - [4-6] A 6 YDR247W VHS1 putative serine/threonine protein kinase VHS1 Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; VHS1 has a paralog, SKS1, that arose from the whole genome duplication mitotic cell cycle, protein phosphorylation kinase activity, transferase activity cytoplasm BY4742 [4-6] A 7 YDR248C - gluconokinase Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 biological_process molecular_function vacuole, cytoplasm BY4742 [4-6] A 8 YDR249C - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [4-6] A 9 YDR250C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-6] A 10 YDR251W PAM1 - Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype; PAM1 has a paralog, SVL3, that arose from the whole genome duplication pseudohyphal growth molecular_function cellular bud, site of polarized growth BY4742 [4-6] A 11 YDR252W BTT1 CCR4-NOT core subunit BTT1 Heterotrimeric nascent polypeptide-associated complex beta3 subunit; complex binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3; BTT1 has a paralog, EGD1, that arose from the whole genome duplication protein targeting, protein folding unfolded protein binding cytoplasm BY4742 [4-6] A 12 YDR253C MET32 - Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; lack of such a loop for MET31 may account for the differential actions of Met32p and Met31p; MET32 has a paralog, MET31, that arose from the whole genome duplication mitotic cell cycle, cellular amino acid metabolic process, transcription from RNA polymerase II promoter, regulation of cell cycle nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding, transcription factor binding nucleus, cytoplasm BY4742 [4-6] B 1 YDR254W CHL4 MCM17|CTF17 Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; forms a stable complex with Iml3p; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, and Mif2p; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 mitotic cell cycle, meiotic cell cycle, organelle fission, regulation of organelle organization, organelle assembly, protein complex biogenesis, chromosome segregation, regulation of cell cycle structural molecule activity nucleus, chromosome BY4742 [4-6] B 2 YDR255C RMD5 ubiquitin-protein ligase RMD5|GID2 Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain carbohydrate metabolic process, protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process transferase activity nucleus, cytoplasm BY4742 [4-6] B 3 YDR256C CTA1 catalase A Catalase A; breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation response to chemical, response to oxidative stress oxidoreductase activity peroxisome, mitochondrion, cytoplasm BY4742 [4-6] B 4 YDR257C RKM4 ribosomal lysine N-methyltransferase|SET7|RMS1 Ribosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein peptidyl-amino acid modification, protein alkylation transferase activity, methyltransferase activity nucleus BY4742 [4-6] B 5 YDR258C HSP78 chaperone ATPase HSP78 Oligomeric mitochondrial matrix chaperone; cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates response to heat, protein folding, mitochondrion organization ATPase activity, hydrolase activity mitochondrion, cytoplasm BY4742 [4-6] B 6 YDR259C YAP6 HAL7 Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding nucleus BY4742 [4-6] B 7 YDR260C SWM1 APC13 Subunit of the anaphase-promoting complex (APC); APC is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation mitotic cell cycle, meiotic cell cycle, organelle fission, sporulation, regulation of organelle organization, proteolysis involved in cellular protein catabolic process, chromosome segregation, protein modification by small protein conjugation or removal, cell wall organization or biogenesis, regulation of cell cycle molecular_function nucleus BY4742 [4-6] B 8 YDR261C EXG2 glucan exo-1,3-beta-glucosidase Exo-1,3-beta-glucanase; involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor cell wall organization or biogenesis hydrolase activity, hydrolase activity, acting on glycosyl bonds cell wall BY4742 [4-6] B 9 YDR262W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment biological_process molecular_function vacuole, cytoplasm BY4742 [4-6] B 10 YDR263C DIN7 exodeoxyribonuclease DIN7|DIN3 Mitochondrial nuclease functioning in DNA repair and replication; modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination; DIN7 has a paralog, EXO1, that arose from the whole genome duplication DNA repair, cellular response to DNA damage stimulus nuclease activity, hydrolase activity mitochondrion, cytoplasm BY4742 [4-6] B 11 YDR264C AKR1 palmitoyltransferase AKR1 Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant response to chemical, protein targeting, protein acylation, regulation of transport, conjugation, signaling, protein lipidation, endocytosis transferase activity endomembrane system, membrane, Golgi apparatus, cytoplasm BY4742 [4-6] B 12 YDR265W PEX10 ubiquitin-protein ligase peroxin 10|PAS4 Peroxisomal membrane E3 ubiquitin ligase; required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders protein targeting, protein modification by small protein conjugation or removal, transmembrane transport, peroxisome organization transferase activity peroxisome, membrane, cytoplasm BY4742 [4-6] C 1 YDR266C HEL2 E3 ubiquitin-protein ligase HEL2 RING finger ubiquitin ligase (E3); involved in ubiquitination and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor chromatin organization, protein modification by small protein conjugation or removal, histone modification transferase activity ribosome, cytoplasm BY4742 [4-6] C 2 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [4-6] C 3 YDR268W MSW1 tryptophan--tRNA ligase MSW1 Mitochondrial tryptophanyl-tRNA synthetase mitochondrial translation, tRNA aminoacylation for protein translation, mitochondrion organization, cellular amino acid metabolic process ligase activity mitochondrion, cytoplasm BY4742 [4-6] C 4 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [4-6] C 5 YDR270W CCC2 Cu(2+)-transporting P-type ATPase CCC2 Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; similar to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism; human homologs ATP7A and ATP7B both complement yeast null mutant transmembrane transport, ion transport, cellular ion homeostasis hydrolase activity, transmembrane transporter activity, ion binding, ATPase activity cytoplasmic vesicle, endomembrane system, membrane, Golgi apparatus, cytoplasm BY4742 [4-6] C 6 - cytoplasmic vesicle, endomembrane system, membrane, Golgi apparatus, cytoplasm cytoplasmic vesicle, endomembrane system, membrane, Golgi apparatus, cytoplasm cytoplasmic vesicle, endomembrane system, membrane, Golgi apparatus, cytoplasm - - - - [4-6] C 7 YDR272W GLO2 hydroxyacylglutathione hydrolase GLO2 Cytoplasmic glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO2 has a paralog, GLO4, that arose from the whole genome duplication monocarboxylic acid metabolic process hydrolase activity cytoplasm BY4742 [4-6] C 8 YDR273W DON1 - Meiosis-specific component of the spindle pole body; subunit of the leading edge protein (LEP) complex (Ssp1-Ady3-Don1-Irc10) that forms a ring-like structure at the leading edge of the prospore membrane (PSM) during meiosis II; required for PSM growth and closure; DON1 has a paralog, CUE5, that arose from the whole genome meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function membrane BY4742 [4-6] C 9 YDR274C - - Putative protein of unknown function; conserved among S. cerevisiae strains; YDR274C is not an essential gene biological_process molecular_function cellular_component BY4742 [4-6] C 10 YDR275W BSC2 - Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; null mutant displays increased translation rate and increased readthrough of premature stop codons; BSC2 has a paralog, IRC23, that arose from the whole genome duplication biological_process molecular_function other BY4742 [4-6] C 11 YDR276C PMP3 SNA1 Small plasma membrane protein; confers resistance to amphotericin B and is a potential target of this common antifungal drug; related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature; not essential for viability; deletion causes hyperpolarization of the plasma membrane potential response to chemical, ion transport ion binding, lipid binding membrane, plasma membrane BY4742 [4-6] C 12 YDR277C MTH1 HTR1|DGT1|BPC1 Negative regulator of the glucose-sensing signal transduction pathway; required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation; MTH1 has a paralog, STD1, that arose from the whole genome duplication carbohydrate transport, signaling molecular_function cellular_component BY4742 [4-6] D 1 YDR278C - - Putative protein of unknown function; conserved among S. cerevisiae strains; YDR278C is not an essential gene biological_process molecular_function cellular_component BY4742 [4-6] D 2 YDR279W RNH202 Rnh2B Ribonuclease H2 subunit; required for RNase H2 activity; role in ribonucleotide excision repair; related to human AGS2 that causes Aicardi-Goutieres syndrome RNA catabolic process, DNA replication nuclease activity, hydrolase activity nucleus BY4742 [4-6] D 3 - nucleus nucleus nucleus - - - - [4-6] D 4 YDR281C PHM6 - Protein of unknown function; expression is regulated by phosphate levels biological_process molecular_function vacuole, cytoplasm BY4742 [4-6] D 5 YDR282C MRX10 - Mitochondrial inner membrane protein of unknown function; associates with mitochondrial ribosome; localizes to the inner membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation biological_process molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-6] D 6 YDR283C GCN2 serine/threonine-protein kinase GCN2|AAS102|NDR2|AAS1 Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control translational initiation, regulation of translation, protein phosphorylation, cellular response to DNA damage stimulus, response to starvation, regulation of cell cycle RNA binding, kinase activity, transferase activity ribosome, cytoplasm BY4742 [4-6] D 7 YDR284C DPP1 bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase|ZRG1 Diacylglycerol pyrophosphate (DGPP) phosphatase; zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism lipid metabolic process phosphatase activity, hydrolase activity membrane, vacuole, cytoplasm BY4742 [4-6] D 8 YDR285W ZIP1 - Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; required for meiotic recombination between non-allelc sites; potential Cdc28p substrate peptidyl-amino acid modification, meiotic cell cycle, organelle fission, regulation of organelle organization, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, DNA recombination, regulation of DNA metabolic process, regulation of cell cycle ubiquitin-like protein binding nucleus, chromosome BY4742 [4-6] D 9 YDR286C - - Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site biological_process molecular_function cellular_component BY4742 [4-6] D 10 YDR287W INM2 inositol monophosphate 1-phosphatase INM2 Inositol monophosphatase; involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy carbohydrate metabolic process phosphatase activity, hydrolase activity cellular_component BY4742 [4-6] D 11 - cellular_component cellular_component cellular_component - - - - [4-6] D 12 YDR289C RTT103 - Protein involved in transcription termination by RNA polymerase II; interacts with exonuclease Rat1p and Rai1p; has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition mRNA processing, cellular response to DNA damage stimulus, transposition enzyme binding nucleus, chromosome BY4742 [4-6] E 1 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [4-6] E 2 YDR291W HRQ1 ATP-dependent 3'-5' DNA helicase 3'-5' DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; acts with Rad4p in nucleotide-excision repair; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome (RTS) DNA repair, cellular response to DNA damage stimulus helicase activity, ATPase activity, hydrolase activity nucleus BY4742 [4-6] E 3 - nucleus nucleus nucleus - - - - [4-6] E 4 YDR293C SSD1 mRNA-binding translational repressor SSD1|SRK1|RLT1|MCS1|CLA1 Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function regulation of translation, cell wall organization or biogenesis RNA binding, mRNA binding nucleus, cellular bud, site of polarized growth, cytoplasm BY4742 [4-6] E 5 YDR294C DPL1 sphinganine-1-phosphate aldolase DPL1|BST1 Dihydrosphingosine phosphate lyase; regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate lipid metabolic process, response to starvation, signaling lyase activity cell cortex, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-6] E 6 YDR295C HDA2 PLO2 Subunit of the HDA1 histone deacetylase complex; possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex contains Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance; relocalizes to the cytosol in response to hypoxia chromatin organization, histone modification, transcription from RNA polymerase II promoter chromatin binding, hydrolase activity, DNA binding nucleus, chromosome, cytoplasm BY4742 [4-6] E 7 YDR296W MHR1 XTC1 Mitochondrial ribosomal protein of the large subunit; also involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress response to chemical, mitochondrial translation, DNA replication, mitochondrion organization, regulation of organelle organization, response to oxidative stress, DNA recombination, regulation of DNA metabolic process structural constituent of ribosome, DNA binding, structural molecule activity nucleus, ribosome, mitochondrion, cytoplasm BY4742 [4-6] E 8 YDR297W SUR2 sphingosine hydroxylase|SYR2 Sphinganine C4-hydroxylase; catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis lipid metabolic process oxidoreductase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-6] E 9 YDR298C ATP5 F1F0 ATP synthase subunit 5|OSC1 Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated transmembrane transport, ion transport, nucleobase-containing small molecule metabolic process hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [4-6] E 10 - mitochondrion, membrane, mitochondrial envelope, cytoplasm mitochondrion, membrane, mitochondrial envelope, cytoplasm mitochondrion, membrane, mitochondrial envelope, cytoplasm - - - - [4-6] E 11 YDR300C PRO1 glutamate 5-kinase Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; required for nitrogen starvation-induced ribophagy but not for nonselective autophagy; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication cellular amino acid metabolic process kinase activity, transferase activity cytoplasm BY4742 [4-6] E 12 - cytoplasm cytoplasm cytoplasm - - - - [4-6] F 1 - - - - - - - - [4-6] F 2 - - - - - - - - [4-6] F 3 YDR304C CPR5 peptidylprolyl isomerase family protein CPR5|CYP5 Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the ER; catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER; CPR5 has a paralog, CPR2, that arose from the whole genome duplication biological_process isomerase activity endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-6] F 4 YDR305C HNT2 bis(5'-adenosyl)-triphosphatase|APH1 Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins nucleobase-containing small molecule metabolic process hydrolase activity nucleus, mitochondrion, cytoplasm BY4742 [4-6] F 5 YDR306C - - F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain proteolysis involved in cellular protein catabolic process protein binding, bridging, transferase activity other BY4742 [4-6] F 6 YDR307W PMT7 putative dolichyl-phosphate-mannose--protein mannosyltransferase Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-6] F 7 - membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm - - - - [4-6] F 8 YDR309C GIC2 - Redundant rho-like GTPase Cdc42p effector; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region; GIC2 has a paralog, GIC1, that arose from the whole genome duplication mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, regulation of cell cycle lipid binding, enzyme regulator activity, enzyme binding, ion binding cellular bud, membrane, site of polarized growth, plasma membrane BY4742 [4-6] F 9 YDR310C SUM1 - Transcriptional repressor that regulates middle-sporulation genes; required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint chromatin organization, DNA replication, regulation of DNA metabolic process, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [4-6] F 10 - nucleus nucleus nucleus - - - - [4-6] F 11 YDR312W SSF2 rRNA-binding ribosome biosynthesis protein Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication ribosome assembly, conjugation, organelle assembly, ribosomal large subunit biogenesis RNA binding, rRNA binding nucleus, nucleolus BY4742 [4-6] F 12 YDR313C PIB1 phosphatidylinositol-3-phosphate-binding ubiquitin-protein ligase RING-type ubiquitin ligase of the endosomal and vacuolar membranes; binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain protein modification by small protein conjugation or removal transferase activity, lipid binding, ion binding cytoplasmic vesicle, endomembrane system, vacuole, membrane, cytoplasm BY4742 [4-6] G 1 YDR314C RAD34 - Protein involved in nucleotide excision repair (NER); homologous to RAD4 DNA repair, cellular response to DNA damage stimulus molecular_function nucleus BY4742 [4-6] G 2 YDR315C IPK1 inositol pentakisphosphate 2-kinase|GSL1 Inositol 1,3,4,5,6-pentakisphosphate 2-kinase; nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable; human IPPK can complement ipk1 null mutant RNA catabolic process kinase activity, transferase activity nucleus BY4742 [4-6] G 3 YDR316W OMS1 putative RNA methyltransferase Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif and is predicted to be an RNA methyltransferase; multicopy suppressor of respiratory defects caused by OXA1 mutations biological_process transferase activity, methyltransferase activity mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-6] G 4 YDR317W HIM1 - Protein of unknown function involved in DNA repair DNA repair, cellular response to DNA damage stimulus molecular_function cellular_component BY4742 [4-6] G 5 YDR318W MCM21 CTF5 Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; modified by sumoylation; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 mitotic cell cycle, meiotic cell cycle, organelle fission, regulation of organelle organization, chromosome segregation, DNA recombination, regulation of DNA metabolic process, regulation of cell cycle molecular_function nucleus, chromosome BY4742 [4-6] G 6 YDR319C YFT2 FIT2A Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens lipid metabolic process molecular_function membrane BY4742 [4-6] G 7 YDR320C SWA2 BUD24|AUX1 Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles organelle inheritance ubiquitin-like protein binding membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-6] G 8 YDR321W ASP1 asparaginase ASP1 Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia; important enzyme for the treatment of acute lymphoblastic leukemia; has low glutaminase activity and dependence; synthesized constitutively monocarboxylic acid metabolic process, cellular amino acid metabolic process hydrolase activity other BY4742 [4-6] G 9 YDR322W MRPL35 mitochondrial 54S ribosomal protein YmL35|YmL35 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [4-6] G 10 YDR323C PEP7 VPT19|VPS19|VPL21|VAC1 Adaptor protein involved in vesicle-mediated vacuolar protein sorting; multivalent adaptor protein; facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance vacuole organization, organelle fusion, vesicle organization, membrane fusion, Golgi vesicle transport, organelle inheritance, exocytosis, endosomal transport lipid binding, ion binding nucleus, cytoplasmic vesicle, endomembrane system, membrane, cytoplasm BY4742 [4-6] G 11 - nucleus, cytoplasmic vesicle, endomembrane system, membrane, cytoplasm nucleus, cytoplasmic vesicle, endomembrane system, membrane, cytoplasm nucleus, cytoplasmic vesicle, endomembrane system, membrane, cytoplasm - - - - [4-6] G 12 - - - - - - - - [4-6] H 1 - - - - - - - - [4-6] H 2 - - - - - - - - [4-6] H 3 - - - - - - - - [4-6] H 4 YDR329C PEX3 PAS3 Peroxisomal membrane protein (PMP); required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p protein targeting, transmembrane transport, peroxisome organization, organelle inheritance protein binding, bridging peroxisome, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [4-6] H 5 YDR330W UBX5 - UBX domain-containing protein that interacts with Cdc48p; ubiquitin regulatory X is also known as UBX proteolysis involved in cellular protein catabolic process molecular_function nucleus, cytoplasm BY4742 [4-6] H 6 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-6] H 7 YDR332W IRC3 double-stranded DNA-dependent ATPase Double-stranded DNA-dependent helicase of the DExH/D-box family; required for maintenance of the mitochondrial (mt) genome; null mutant accumulates double-stranded breaks in mt DNA; localizes to the mt matrix mitochondrion organization ATPase activity, hydrolase activity mitochondrion, cytoplasm BY4742 [4-6] H 8 YDR333C RQC1 - Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Rkr1p-Tae2p-Cdc48p-Npl4p-Ufd1p) is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Rkr1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC regulation of translation, translational elongation, proteolysis involved in cellular protein catabolic process molecular_function ribosome, cytoplasm BY4742 [4-6] H 9 YDR334W SWR1 chromatin-remodeling protein SWR1 Swi2/Snf2-related ATPase; structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; relocalizes to the cytosol in response to hypoxia; chronological aging factor that mediates lifespan extension by dietary restriction chromatin organization structural molecule activity nucleus, chromosome, cytoplasm BY4742 [4-6] H 10 YDR335W MSN5 karyopherin MSN5|STE21|KAP142 Karyopherin; involved in nuclear import and export of proteins, including import of replication protein A and export of Far1p and transcription factors Swi5p, Swi6p, Msn2p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm; exportin-5 homolog nucleobase-containing compound transport, nuclear transport protein transporter activity nucleus, membrane, cytoplasm BY4742 [4-6] H 11 YDR336W MRX8 - Protein that associates with mitochondrial ribosome; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene biological_process molecular_function cellular_component BY4742 [4-6] H 12 YDR337W MRPS28 mitochondrial 37S ribosomal protein MRPS28 Mitochondrial ribosomal protein of the small subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [4-7] A 1 YDR338C - - Putative protein of unknown function; member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily response to chemical transmembrane transporter activity membrane BY4742 [4-7] A 2 - membrane membrane membrane - - - - [4-7] A 3 YDR340W - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [4-7] A 4 - cellular_component cellular_component cellular_component - - - - [4-7] A 5 YDR344C - - Putative protein of unknown function; conserved among S. cerevisiae strains biological_process molecular_function cellular_component BY4742 [4-7] A 6 YDR345C HXT3 hexose transporter HXT3 Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication transmembrane transport, carbohydrate transport transmembrane transporter activity membrane, plasma membrane BY4742 [4-7] A 7 YDR346C SVF1 SGI1 Protein with a potential role in cell survival pathways; required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance response to chemical, response to oxidative stress molecular_function nucleus, cytoplasm BY4742 [4-7] A 8 YDR347W MRP1 mitochondrial 37S ribosomal protein MRP1 Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [4-7] A 9 YDR348C PAL1 - Protein of unknown function thought to be involved in endocytosis; physically interacts with Ede1p and is found at endocytic sites at cell periphery during early stages of endocytosis; green fluorescent protein (GFP)-fusion protein localizes to bud neck; potential Cdc28p substrate; similar to S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress; PAL1 has a paralog, YHR097C, that arose from the whole genome duplication biological_process molecular_function cellular bud, cell cortex, site of polarized growth, cytoplasm BY4742 [4-7] A 10 YDR349C YPS7 putative aspartic endopeptidase Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum cell wall organization or biogenesis peptidase activity, hydrolase activity endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-7] A 11 YDR350C ATP22 TCM10 Specific translational activator for the mitochondrial ATP6 mRNA; Atp6p encodes a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane mitochondrial translation, regulation of translation, mitochondrion organization, regulation of organelle organization, protein complex biogenesis other mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [4-7] A 12 YDR351W SBE2 - Protein required for bud growth; involved in transport of cell wall components from the Golgi to the cell surface; SBE2 has a paralog, SBE22, that arose from the whole genome duplication cell wall organization or biogenesis molecular_function endomembrane system, cytoplasm, Golgi apparatus BY4742 [4-7] B 1 YDR352W YPQ2 - Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter transmembrane transport, ion transport, amino acid transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [4-7] B 2 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [4-7] B 3 YDR354W TRP4 anthranilate phosphoribosyltransferase Anthranilate phosphoribosyl transferase; transferase of the tryptophan biosynthetic pathway; catalyzes the phosphoribosylation of anthranilate; subject to the general control system of amino acid biosynthesis cellular amino acid metabolic process transferase activity, transferring glycosyl groups, transferase activity nucleus, cytoplasm BY4742 [4-7] B 4 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-7] B 5 - - - - - - - - [4-7] B 6 YDR357C CNL1 BLC1 Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm vesicle organization molecular_function cytoplasm BY4742 [4-7] B 7 YDR358W GGA1 ubiquitin-binding protein Golgi-localized protein with homology to gamma-adaptin; interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi; GGA1 has a paralog, GGA2, that arose from the whole genome duplication Golgi vesicle transport, protein targeting, endosomal transport, proteolysis involved in cellular protein catabolic process ubiquitin-like protein binding endomembrane system, cytoplasm, Golgi apparatus BY4742 [4-7] B 8 YDR359C EAF1 VID21 Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 DNA repair, chromatin organization, cellular response to DNA damage stimulus, protein complex biogenesis molecular_function nucleus BY4742 [4-7] B 9 YDR360W OPI7 - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C biological_process molecular_function cellular_component BY4742 [4-7] B 10 - cellular_component cellular_component cellular_component - - - - [4-7] B 11 - - - - - - - - [4-7] B 12 YDR363W ESC2 - Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member peptidyl-amino acid modification, mitotic cell cycle, DNA repair, organelle fission, chromatin organization, cellular response to DNA damage stimulus, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, DNA recombination, regulation of cell cycle molecular_function nucleus BY4742 [4-7] C 1 YDR364C CDC40 SLU4|SLT15|PRP17 Pre-mRNA splicing factor; important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression, particularly at the G1/S phase transition; required for DNA synthesis during mitosis and meiosis; has WD repeats; thermosensitivity of the cdc40 null mutant is functionally complemented by a chimeric construct containing the N-terminal 156 amino acids of yeast Cdc40p fused to the C-terminal two thirds (297 amino acids) of human CDC40 RNA splicing, mRNA processing other nucleus BY4742 [4-7] C 2 - nucleus nucleus nucleus - - - - [4-7] C 3 - - - - - - - - [4-7] C 4 YDR368W YPR1 trifunctional aldehyde reductase/carbonyl reductase (NADPH)/glucose 1-dehydrogenase (NADP(+)) YPR1 NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication; human homolog AKR1B1 can complement yeast null mutant carbohydrate metabolic process, response to chemical, response to oxidative stress oxidoreductase activity nucleus, cytoplasm BY4742 [4-7] C 5 YDR369C XRS2 - Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling meiotic cell cycle, DNA repair, telomere organization, organelle fission, sporulation, cellular response to DNA damage stimulus, DNA recombination protein binding, bridging, DNA binding nucleus BY4742 [4-7] C 6 YDR370C DXO1 - mRNA 5'-end-capping quality-control protein; has distributive, 5'-3' exoRNase activity; similar to Rai1p; RNA catabolic process nuclease activity, hydrolase activity cytoplasm BY4742 [4-7] C 7 YDR371W CTS2 putative chitinase Putative chitinase; functionally complements A. gossypii cts2 mutant sporulation defect sporulation molecular_function cytoplasm BY4742 [4-7] C 8 YDR372C VPS74 MNN3|API1 Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 response to chemical, Golgi vesicle transport, lipid metabolic process, signaling lipid binding, enzyme binding, ion binding nucleus, endomembrane system, membrane, Golgi apparatus, cytoplasm BY4742 [4-7] C 9 - nucleus, endomembrane system, membrane, Golgi apparatus, cytoplasm nucleus, endomembrane system, membrane, Golgi apparatus, cytoplasm nucleus, endomembrane system, membrane, Golgi apparatus, cytoplasm - - - - [4-7] C 10 YDR374C PHO92 mRNA-binding phosphate metabolism regulator Posttranscriptional regulator of phosphate metabolism; facilitates PHO4 mRNA degradation by interacting with Pop2p; regulates PHO4 mRNA stability by binding to PHO4's 3'UTR in a phosphate-dependent manner; contains highly conserved YTH (YT521-B Homology) domain that exhibits RNA-binding activity; human homolog YTHDF2 can complement yeast null mutant other RNA binding, mRNA binding cytoplasm BY4742 [4-7] C 11 YDR375C BCS1 bifunctional AAA family ATPase chaperone/translocase BCS1 Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane and delivers it to an assembly intermediate of respiratory Complex III; also required for assembly of the Qcr10p subunit; mutation is functionally complemented by human homolog BCS1L, linked to neonatal diseases mitochondrion organization, protein complex biogenesis protein transporter activity, hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [4-7] C 12 - mitochondrion, membrane, mitochondrial envelope, cytoplasm mitochondrion, membrane, mitochondrial envelope, cytoplasm mitochondrion, membrane, mitochondrial envelope, cytoplasm - - - - [4-7] D 1 YDR377W ATP17 F1F0 ATP synthase subunit f Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis transmembrane transport, ion transport, nucleobase-containing small molecule metabolic process hydrolase activity, transmembrane transporter activity, ATPase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [4-7] D 2 YDR378C LSM6 U4/U6-U5 snRNP complex subunit LSM6 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA RNA splicing, rRNA processing, ribosomal small subunit biogenesis, mRNA processing RNA binding nucleus, nucleolus, cytoplasm BY4742 [4-7] D 3 YDR379W RGA2 - GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p; RGA2 has a paralog, RGA1, that arose from the whole genome duplication cytoskeleton organization, signaling enzyme regulator activity cell cortex, cytoskeleton, cellular bud, site of polarized growth, cytoplasm BY4742 [4-7] D 4 YDR380W ARO10 phenylpyruvate decarboxylase ARO10 Phenylpyruvate decarboxylase; catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway; involved in protein N-terminal Met and Ala catabolism cellular amino acid metabolic process lyase activity cytoplasm BY4742 [4-7] D 5 - cytoplasm cytoplasm cytoplasm - - - - [4-7] D 6 YDR382W RPP2B ribosomal protein P2B|YP2beta|P2B|L45|YPA1|RPL45 Ribosomal protein P2 beta; a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P2 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm cytoplasmic translation, regulation of protein modification process, protein phosphorylation structural molecule activity, structural constituent of ribosome, enzyme regulator activity vacuole, ribosome, cytoplasm BY4742 [4-7] D 7 YDR383C NKP1 - Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 biological_process molecular_function chromosome BY4742 [4-7] D 8 YDR384C ATO3 putative ammonium permease ATO3 Plasma membrane protein, putative ammonium transporter; regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrion, plasma membrane, cytoplasm BY4742 [4-7] D 9 YDR385W EFT2 elongation factor 2 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication regulation of translation, translational elongation RNA binding, translation factor activity, RNA binding ribosome, cytoplasm BY4742 [4-7] D 10 YDR386W MUS81 SLX3 Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); helix-hairpin-helix protein; phosphorylation of non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates function of Mms4p-Mus81p meiotic cell cycle, DNA repair, organelle fission, cellular response to DNA damage stimulus, chromosome segregation, DNA recombination hydrolase activity, enzyme regulator activity, nuclease activity nucleus BY4742 [4-7] D 11 YDR387C CIN10 - Putative transporter; member of the sugar porter family; non-essential gene; overexpression results in elevated colony sectoring, an indicator of chromosomal instability transmembrane transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [4-7] D 12 YDR388W RVS167 amphiphysin Calmodulin-binding actin-associated protein; roles in endocytic membrane tabulation and constriction, and exocytosis; N-BAR domain protein that interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin endocytosis cytoskeletal protein binding, lipid binding cell cortex, site of polarized growth, cytoskeleton, cytoplasm BY4742 [4-7] E 1 YDR389W SAC7 - GTPase activating protein (GAP) for Rho1p; regulator of a Tor2p-mediated, Rho1p GTPase switch that controls organization of the actin cytoskeleton; negative regulator of the RHO1-PKC1-MAPK cell integrity (CWI) and membrane fluidity homeostasis signaling pathways; potential Cdc28p substrate; SAC7 has a paralog, BAG7, that arose from the whole genome duplication cytoskeleton organization, regulation of organelle organization, cell wall organization or biogenesis, signaling enzyme regulator activity cell cortex, cytoplasm BY4742 [4-7] E 2 - cell cortex, cytoplasm cell cortex, cytoplasm cell cortex, cytoplasm - - - - [4-7] E 3 YDR391C - - Putative protein of unknown function; possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm BY4742 [4-7] E 4 YDR392W SPT3 transcriptional regulator SPT3 Subunit of the SAGA and SAGA-like transcriptional regulatory complexes; interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters; relocalizes to the cytosol in response to hypoxia peptidyl-amino acid modification, protein acylation, invasive growth in response to glucose limitation, chromatin organization, histone modification, pseudohyphal growth, transcription from RNA polymerase II promoter transcription factor activity, protein binding nucleus, cytoplasm BY4742 [4-7] E 5 YDR393W SHE9 MDM33 Protein required for normal mitochondrial morphology; mitochondrial inner membrane protein; may be involved in fission of the inner membrane; forms a homo-oligomeric complex mitochondrion organization molecular_function membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-7] E 6 - membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm membrane, mitochondrial envelope, mitochondrion, cytoplasm - - - - [4-7] E 7 YDR395W SXM1 KAP108 Nuclear transport factor (karyopherin); involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 nucleobase-containing compound transport, nuclear transport protein transporter activity nucleus, membrane, cytoplasm BY4742 [4-7] E 8 - nucleus, membrane, cytoplasm nucleus, membrane, cytoplasm nucleus, membrane, cytoplasm - - - - [4-7] E 9 - - - - - - - - [4-7] E 10 - - - - - - - - [4-7] E 11 YDR399W HPT1 hypoxanthine phosphoribosyltransferase|HGPRTase|HPRT|BRA6 Dimeric hypoxanthine-guanine phosphoribosyltransferase; catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome nucleobase-containing small molecule metabolic process transferase activity, transferring glycosyl groups, transferase activity nucleus, cytoplasm BY4742 [4-7] E 12 YDR400W URH1 trifunctional uridine nucleosidase/nicotinamide riboside hydrolase/nicotinic acid riboside hydrolase Uridine nucleosidase (uridine-cytidine N-ribohydrolase); cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways cofactor metabolic process, nucleobase-containing small molecule metabolic process hydrolase activity, hydrolase activity, acting on glycosyl bonds nucleus, cytoplasm BY4742 [4-7] F 1 YDR401W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-7] F 2 YDR402C DIT2 putative cytochrome P450|CYP56 N-formyltyrosine oxidase; sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively meiotic cell cycle, sporulation, cell wall organization or biogenesis oxidoreductase activity cellular_component BY4742 [4-7] F 3 YDR403W DIT1 - Sporulation-specific enzyme required for spore wall maturation; involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure meiotic cell cycle, sporulation, cell wall organization or biogenesis other cellular_component BY4742 [4-7] F 4 - cellular_component cellular_component cellular_component - - - - [4-7] F 5 YDR405W MRP20 mitochondrial 54S ribosomal protein YmL41|YmL41|MRPL41 Mitochondrial ribosomal protein of the large subunit mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [4-7] F 6 YDR406W PDR15 ATP-binding cassette multidrug transporter PDR15 Plasma membrane ATP binding cassette (ABC) transporter; multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element; PDR15 has a paralog, PDR5, that arose from the whole genome duplication response to chemical ATPase activity, hydrolase activity, transmembrane transporter activity membrane BY4742 [4-7] F 7 - membrane membrane membrane - - - - [4-7] F 8 YDR408C ADE8 phosphoribosylglycinamide formyltransferase Phosphoribosyl-glycinamide transformylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway nucleobase-containing small molecule metabolic process transferase activity nucleus, cytoplasm BY4742 [4-7] F 9 YDR409W SIZ1 SUMO ligase SIZ1|ULL1 SUMO E3 ligase; promotes attachment of small ubiquitin-related modifier sumo (Smt3p) to primarily cytoplasmic proteins; regulates Rsp5p ubiquitin ligase activity and is in turn itself regulated by Rsp5p; required for sumoylation of septins and histone H3 variant Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; localizes to the septin ring; acts as an adapter between E2, Ubc9p and substrates; tends to compensate for survival of DNA damage in absence of Nfi1p peptidyl-amino acid modification, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal transferase activity nucleus, cell cortex, cytoskeleton, chromosome, cytoplasm BY4742 [4-7] F 10 YDR410C STE14 protein-S-isoprenylcysteine carboxyl O-methyltransferase Farnesyl cysteine-carboxyl methyltransferase; mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane protein alkylation, protein maturation transferase activity, methyltransferase activity nucleus, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [4-7] F 11 YDR411C DFM1 - Endoplasmic reticulum (ER) localized protein; involved in ER-associated protein degradation (ERAD), ER stress, and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p response to chemical, proteolysis involved in cellular protein catabolic process, signaling molecular_function endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [4-7] F 12 - endomembrane system, membrane, endoplasmic reticulum, cytoplasm endomembrane system, membrane, endoplasmic reticulum, cytoplasm endomembrane system, membrane, endoplasmic reticulum, cytoplasm - - - - [4-7] G 1 - - - - - - - - [4-7] G 2 YDR414C ERD1 LDB2 Predicted membrane protein required for lumenal ER protein retention; mutants secrete the endogenous ER protein, BiP (Kar2p) protein glycosylation molecular_function membrane BY4742 [4-7] G 3 YDR415C - putative aminopeptidase Putative aminopeptidase; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to the vacuole biological_process molecular_function vacuole, cytoplasm BY4742 [4-7] G 4 - vacuole, cytoplasm vacuole, cytoplasm vacuole, cytoplasm - - - - [4-7] G 5 - - - - - - - - [4-7] G 6 YDR418W RPL12B uL11|ribosomal 60S subunit protein L12B|L11|YL23|L15B|L12B Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [4-7] G 7 YDR419W RAD30 DNA-directed DNA polymerase eta|DBH1 DNA polymerase eta; involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; involved in formation of post-replicative damage-induced genome-wide cohesion; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV mitotic cell cycle, DNA repair, organelle fission, cellular response to DNA damage stimulus, chromosome segregation transferase activity, nucleotidyltransferase activity nucleus, chromosome, mitochondrion, cytoplasm BY4742 [4-7] G 8 YDR420W HKR1 - Mucin family member that functions as an osmosensor in the HOG pathway; large, highly glycosylated protein that functions redundantly with Msb2p in Sho1p-mediated osmostresss induction of the HOG signaling pathway; cytoplasmic domain interacts with Ahk1p a scaffold protein that prevents cross talk with the Kss1p MAPK of the filamentous growth pathway; mutant displays defects in beta-1,3 glucan synthesis and bud site selection mitotic cell cycle, carbohydrate metabolic process, cytokinesis, cell wall organization or biogenesis, signaling, response to osmotic stress signal transducer activity membrane, site of polarized growth, plasma membrane BY4742 [4-7] G 9 YDR421W ARO80 - Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids transcription from RNA polymerase II promoter, cellular amino acid metabolic process nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [4-7] G 10 YDR422C SIP1 - Alternate beta-subunit of the Snf1p kinase complex; may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions protein phosphorylation, signaling, protein complex biogenesis kinase activity, transferase activity vacuole, cytoplasm BY4742 [4-7] G 11 YDR423C CAD1 YAP2 AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [4-7] G 12 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-7] H 1 YDR425W SNX41 - Sorting nexin; involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p endosomal transport ion binding, lipid binding cytoplasmic vesicle, endomembrane system, cytoplasm BY4742 [4-7] H 2 YDR426C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 biological_process molecular_function cellular_component BY4742 [4-7] H 3 - cellular_component cellular_component cellular_component - - - - [4-7] H 4 YDR428C BNA7 arylformamidase Formylkynurenine formamidase; involved in the de novo biosynthesis of NAD from tryptophan via kynurenine cofactor metabolic process, nucleobase-containing small molecule metabolic process hydrolase activity cellular_component BY4742 [4-7] H 5 - cellular_component cellular_component cellular_component - - - - [4-7] H 6 YDR430C CYM1 MOP112 Lysine-specific metalloprotease of the pitrilysin family; metalloprotease of the intermembrane space; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology proteolysis involved in cellular protein catabolic process peptidase activity, hydrolase activity mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-7] H 7 YDR431W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-7] H 8 YDR432W NPL3 mRNA-binding protein NPL3|NAB1|NOP3|MTS1|MTR13 RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ubiquitin ligase Bre1p, linking histone ubiquitination to mRNA processing; may have role in telomere maintenance; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in cytoplasm; protein abundance increases in response to DNA replication stress RNA splicing, regulation of translation, nucleobase-containing compound transport, DNA-templated transcription, termination, mRNA processing, transcription from RNA polymerase II promoter, nuclear transport, DNA-templated transcription, elongation RNA binding, enzyme binding, mRNA binding nucleus, cytoplasm BY4742 [4-7] H 9 YDR433W - KRE22 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data cellular response to DNA damage stimulus molecular_function cellular_component BY4742 [4-7] H 10 - cellular_component cellular_component cellular_component - - - - [4-7] H 11 YDR435C PPM1 leucine carboxy methyltransferase Carboxyl methyltransferase; methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits; required for methionine to inhibit autophagy and promote growth protein alkylation, protein complex biogenesis transferase activity, methyltransferase activity cellular_component BY4742 [4-7] H 12 YDR436W PPZ2 salt homeostasis regulator Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance cellular ion homeostasis phosphatase activity, hydrolase activity cellular_component BY4742 [4-8] A 1 - cellular_component cellular_component cellular_component - - - - [4-8] A 2 YDR438W THI74 - Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication; shows sequence homology to human gene SLC35F3, a thiamine transporter implicated in hypertension biological_process molecular_function membrane, vacuole, mitochondrion, cytoplasm BY4742 [4-8] A 3 YDR439W LRS4 - Nucleolar protein that forms a complex with Csm1p; and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation meiotic cell cycle, organelle fission, chromatin organization, chromosome segregation, nucleus organization molecular_function nucleus, chromosome, nucleolus BY4742 [4-8] A 4 YDR440W DOT1 histone methyltransferase DOT1|KMT4|PCH1 Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response peptidyl-amino acid modification, mitotic cell cycle, meiotic cell cycle, DNA repair, protein alkylation, organelle fission, chromatin organization, regulation of organelle organization, cellular response to DNA damage stimulus, histone modification, DNA recombination, regulation of cell cycle chromatin binding, transferase activity, histone binding, methyltransferase activity nucleus BY4742 [4-8] A 5 YDR441C APT2 adenine phosphoribosyltransferase APT2 Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication biological_process molecular_function cytoplasm BY4742 [4-8] A 6 YDR442W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-8] A 7 YDR443C SSN2 SSX5|MED13|RYE3|UME2|SRB9|SCA1|NUT8 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation response to chemical, transcription from RNA polymerase II promoter transcription factor activity, protein binding nucleus BY4742 [4-8] A 8 YDR446W ECM11 - Meiosis-specific protein; component of the Synaptonemal Complex (SC) along with Gmc2p; required for efficient crossover formation and for the efficient loading of the SC transverse filament protein, Zip1p; is SUMOlytaed in a Gmc2p manner, and SUMOylation is required for its function in meiosis; GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure meiotic cell cycle, organelle fission, DNA recombination molecular_function nucleus, chromosome BY4742 [4-8] A 9 YDR447C RPS17B eS17|ribosomal 40S subunit protein S17B|S17e|rp51B|S17B|RPL51B|RP51B Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation, organelle assembly, ribosomal small subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [4-8] A 10 YDR448W ADA2 chromatin-binding transcription regulator ADA2|SWI8 Transcription coactivator; component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification, regulation of protein modification process, transcription from RNA polymerase II promoter chromatin binding, transferase activity, lipid binding, ion binding, transcription factor activity, protein binding nucleus BY4742 [4-8] A 11 - nucleus nucleus nucleus - - - - [4-8] A 12 YDR450W RPS18A uS13|ribosomal 40S subunit protein S18A|S18A|S13 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress cytoplasmic translation, rRNA processing, nucleobase-containing compound transport, ribosomal small subunit biogenesis, nuclear transport structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm BY4742 [4-8] B 1 YDR451C YHP1 - Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication mitotic cell cycle, transcription from RNA polymerase II promoter DNA binding, transcription factor activity, protein binding nucleus, chromosome BY4742 [4-8] B 2 YDR452W PPN1 endopolyphosphatase|PHM5 Dual endo- and exopolyphosphatase with a role in phosphate metabolism; acts as both an endopolyphosphatase cleaving long chains of polyphosphate distributively to generate shorter polymer chains and as an exopolyphosphatase catalyzing the hydrolysis of terminal phosphate from polyphosphate; localizes to the vacuole, nucleus and cytosol; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress other hydrolase activity nucleus, membrane, vacuole, cytoplasm BY4742 [4-8] B 3 YDR453C TSA2 thioredoxin peroxidase TSA2|cTPxII Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress; TSA2 has a paralog, TSA1, that arose from the whole genome duplication response to chemical, protein folding, response to oxidative stress oxidoreductase activity cytoplasm BY4742 [4-8] B 4 - cytoplasm cytoplasm cytoplasm - - - - [4-8] B 5 YDR455C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W biological_process molecular_function cellular_component BY4742 [4-8] B 6 YDR456W NHX1 bifunctional K:H/Na:H antiporter NHX1|VPL27|NHA2|VPS44 Na+/H+ and K+/H+ exchanger; required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion; ortholog of human NHE9, which is linked to autism transmembrane transport, ion transport, cellular ion homeostasis transmembrane transporter activity cytoplasmic vesicle, endomembrane system, vacuole, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4742 [4-8] B 7 YDR457W TOM1 E3 ubiquitin-protein ligase TOM1 E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase mitotic cell cycle, organelle fission, rRNA processing, ribosomal large subunit biogenesis, proteolysis involved in cellular protein catabolic process, ribosomal small subunit biogenesis, protein modification by small protein conjugation or removal, nuclear transport, nucleus organization transferase activity nucleus, membrane, nucleolus BY4742 [4-8] B 8 YDR458C HEH2 - Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle; HEH2 has a paralog, SRC1, that arose from the whole genome duplication biological_process molecular_function nucleus, endomembrane system BY4742 [4-8] B 9 YDR459C PFA5 palmitoyltransferase PFA5 Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain protein acylation, protein lipidation transferase activity membrane, plasma membrane BY4742 [4-8] B 10 - membrane, plasma membrane membrane, plasma membrane membrane, plasma membrane - - - - [4-8] B 11 - - - - - - - - [4-8] B 12 YDR462W MRPL28 mitochondrial 54S ribosomal protein YmL28|YmL28 Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [4-8] C 1 YDR463W STP1 SSY2|BAP1 Transcription factor; contains a N-terminal regulatory motif (RI) that acts as a cytoplasmic retention determinant and as an Asi dependent degron in the nucleus; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, membrane, plasma membrane, cytoplasm BY4742 [4-8] C 2 - nucleus, membrane, plasma membrane, cytoplasm nucleus, membrane, plasma membrane, cytoplasm nucleus, membrane, plasma membrane, cytoplasm - - - - [4-8] C 3 YDR465C RMT2 protein-arginine N5-methyltransferase Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress peptidyl-amino acid modification, protein alkylation transferase activity, methyltransferase activity nucleus, cytoplasm BY4742 [4-8] C 4 YDR466W PKH3 protein kinase PKH3 Protein kinase with similarity to mammalian PDK1 and yeast Pkh1p/Phk2p; yeast Pkh1p and Pkh2p are two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant protein phosphorylation, cell wall organization or biogenesis, signaling kinase activity, transferase activity cellular_component BY4742 [4-8] C 5 YDR467C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-8] C 6 - cellular_component cellular_component cellular_component - - - - [4-8] C 7 YDR469W SDC1 SAF19|CPS25 Subunit of the COMPASS (Set1C) complex; COMPASS methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 peptidyl-amino acid modification, protein alkylation, chromatin organization, histone modification transferase activity, methyltransferase activity nucleus BY4742 [4-8] C 8 YDR470C UGO1 - Outer membrane component of the mitochondrial fusion machinery; binds to Fzo1p and Mgm1p to link these two GTPases during mitochondrial fusion; involved in fusion of both the outer and inner membranes; facilitates dimerization of Fzo1p during fusion; import into the outer membrane is mediated by Tom70p and Mim1p; has similarity to carrier proteins but likely not a transporter; similar to human SLC25A46 implicated in optic atroprophy spectrum disorder mitochondrion organization, organelle fusion, membrane fusion molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [4-8] C 9 YDR471W RPL27B eL27|ribosomal 60S subunit protein L27B|L27e|L27B Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [4-8] C 10 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [4-8] C 11 - - - - - - - - [4-8] C 12 YDR474C - - - - - - BY4742 [4-8] D 1 - - - - - - - - [4-8] D 2 YDR476C - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-8] D 3 YDR477W SNF1 AMP-activated serine/threonine-protein kinase catalytic subunit SNF1|PAS14|HAF3|GLC2|CCR1|CAT1 AMP-activated S/T protein kinase; forms a complex with Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; regulates nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth and acts as a non-canonical GEF, activating Arf3p during invasive growth; SUMOylation by Mms21p inhibits its function and targets Snf1p for destruction via the Slx5-Slx8 Ub ligase response to chemical, regulation of translation, cell wall organization or biogenesis, carbohydrate metabolic process, invasive growth in response to glucose limitation, protein phosphorylation, pseudohyphal growth, response to starvation guanyl-nucleotide exchange factor activity, kinase activity, transferase activity nucleus, vacuole, mitochondrion, endomembrane system, cytoplasm BY4742 [4-8] D 4 - nucleus, vacuole, mitochondrion, endomembrane system, cytoplasm nucleus, vacuole, mitochondrion, endomembrane system, cytoplasm nucleus, vacuole, mitochondrion, endomembrane system, cytoplasm - - - - [4-8] D 5 YDR479C PEX29 - ER-resident protein involved in peroxisomal biogenesis; ER-localized protein that associates with peroxisomes; interacts with Pex30p and reticulons Rtn1p and Yop1p to regulate peroxisome biogenesis from the ER; role in peroxisomal-destined vesicular flow from the ER; regulates peroxisomal size, number and distribution; Pex28p and Pex29p may act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress peroxisome organization molecular_function peroxisome, membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-8] D 6 YDR480W DIG2 RST2 MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG2 has a paralog, DIG1, that arose from the whole genome duplication response to chemical, invasive growth in response to glucose limitation, pseudohyphal growth, transcription from RNA polymerase II promoter transcription factor binding nucleus BY4742 [4-8] D 7 YDR481C PHO8 alkaline phosphatase PHO8|phoH Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN cofactor metabolic process, protein dephosphorylation, nucleobase-containing small molecule metabolic process phosphatase activity, hydrolase activity membrane, vacuole, cytoplasm BY4742 [4-8] D 8 YDR482C CWC21 U2-type spliceosomal complex subunit CWC21 Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p RNA splicing, mRNA processing molecular_function nucleus BY4742 [4-8] D 9 YDR483W KRE2 alpha-1,2-mannosyltransferase KRE2|MNT1 Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication protein glycosylation, cell wall organization or biogenesis transferase activity, transferring glycosyl groups, transferase activity endomembrane system, vacuole, membrane, Golgi apparatus, cytoplasm BY4742 [4-8] D 10 YDR484W VPS52 SAC2 Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; involved in localization of actin and chitin; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p Golgi vesicle transport, endosomal transport other cytoplasmic vesicle, endomembrane system, cytoplasm, Golgi apparatus BY4742 [4-8] D 11 YDR485C VPS72 SWC2 Htz1p-binding component of the SWR1 complex; exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; may function as a lock that prevents removal of H2AZ from nucleosomes; required for vacuolar protein sorting chromatin organization histone binding nucleus, chromosome, cytoplasm BY4742 [4-8] D 12 YDR486C VPS60 CHM5|MOS10 Protein involved in late endosome to vacuole transport; cytoplasmic and vacuolar membrane protein; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p endosomal transport molecular_function membrane, vacuole, cytoplasm BY4742 [4-8] E 1 - membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm membrane, vacuole, cytoplasm - - - - [4-8] E 2 YDR488C PAC11 dynein intermediate chain Dynein intermediate chain, microtubule motor protein; required for intracellular transport and cell division; acts in cytoplasmic dynein pathway; forms complex with dynein light chain Dyn2p that promotes Dyn1p homodimerization and potentiates motor processivity; Dyn2p-Pac11p complex is also important for interaction of dynein motor complex with dynactin complex; forms cortical cytoplasmic microtubule capture site with Num1p; essential in the absence of CIN8 mitotic cell cycle, cytoskeleton organization ATPase activity, hydrolase activity cytoskeleton, cytoplasm BY4742 [4-8] E 3 - cytoskeleton, cytoplasm cytoskeleton, cytoplasm cytoskeleton, cytoplasm - - - - [4-8] E 4 YDR490C PKH1 serine/threonine protein kinase PKH1 Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh2p; PKH1 has a paralog, PKH2, that arose from the whole genome duplication peptidyl-amino acid modification, protein phosphorylation, organelle assembly, RNA catabolic process, endosomal transport, cell wall organization or biogenesis, signaling, endocytosis kinase activity, transferase activity cell cortex, cytoplasm BY4742 [4-8] E 5 YDR491C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane BY4742 [4-8] E 6 YDR492W IZH1 PAQR-type receptor Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc; IZH1 has a paralog, IZH4, that arose from the whole genome duplication cellular ion homeostasis molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-8] E 7 - membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm - - - - [4-8] E 8 YDR494W RSM28 mitochondrial 37S ribosomal protein RSM28 Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation translational initiation, mitochondrial translation, mitochondrion organization structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm BY4742 [4-8] E 9 YDR495C VPS3 CORVET complex subunit VPS3|VPT17|VPL3|PEP6 Component of CORVET membrane tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase vacuole organization, protein targeting, cellular ion homeostasis, organelle inheritance molecular_function cytoplasmic vesicle, endomembrane system, cytoplasm BY4742 [4-8] E 10 YDR496C PUF6 - Pumilio-homology domain protein; binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; co-sediments with the 60S ribosomal subunit and is required for its biogenesis regulation of translation, ribosomal large subunit biogenesis RNA binding, mRNA binding nucleus, ribosome, nucleolus, cytoplasm BY4742 [4-8] E 11 YDR497C ITR1 myo-inositol transporter ITR1 Myo-inositol transporter; member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress; ITR1 has a paralog, ITR2, that arose from the whole genome duplication transmembrane transport, pseudohyphal growth transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [4-8] E 12 - membrane, vacuole, plasma membrane, cytoplasm membrane, vacuole, plasma membrane, cytoplasm membrane, vacuole, plasma membrane, cytoplasm - - - - [4-8] F 1 - - - - - - - - [4-8] F 2 - - - - - - - - [4-8] F 3 YDR501W PLM2 - Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication cellular response to DNA damage stimulus chromatin binding nucleus, chromosome BY4742 [4-8] F 4 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [4-8] F 5 YDR503C LPP1 phosphatidate phosphatase LPP1 Lipid phosphate phosphatase; catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA lipid metabolic process phosphatase activity, hydrolase activity membrane BY4742 [4-8] F 6 YDR504C SPG3 - Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-8] F 7 YDR505C PSP1 GIN5 Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication biological_process RNA binding, mRNA binding mitochondrion, cytoplasm BY4742 [4-8] F 8 YDR506C GMC1 putative oxidoreductase Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively meiotic cell cycle, organelle fission molecular_function cellular_component BY4742 [4-8] F 9 YDR507C GIN4 protein kinase GIN4|ERC47 Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication mitotic cell cycle, protein phosphorylation, cell budding, cytoskeleton organization, organelle assembly, protein complex biogenesis, regulation of cell cycle kinase activity, transferase activity cellular bud, site of polarized growth BY4742 [4-8] F 10 YDR508C GNP1 glutamine permease GNP1 High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication transmembrane transport, ion transport, amino acid transport transmembrane transporter activity endomembrane system, mitochondrion, cellular bud, membrane, site of polarized growth, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [4-8] F 11 YDR509W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [4-8] F 12 - cellular_component cellular_component cellular_component - - - - [4-8] G 1 YDR511W SDH7 ACN9 Mitochondrial protein involved in assembly of succinate dehydrogenase; has a role in maturation of the Sdh2p subunit; localized to the mitochondrial intermembrane space; required for acetate utilization and gluconeogenesis; mutation in Drosophila ortholog SDHAF3 causes reduced succinate dehydrogenase activity and neuronal and muscular dysfunction; member of the LYR protein family carbohydrate metabolic process, mitochondrion organization, protein complex biogenesis molecular_function mitochondrial envelope, mitochondrion, cytoplasm BY4742 [4-8] G 2 YDR512C EMI1 - Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs; deletion affects mitochondrial morphology meiotic cell cycle, sporulation, mitochondrion organization, transcription from RNA polymerase II promoter molecular_function cellular_component BY4742 [4-8] G 3 YDR513W GRX2 dithiol glutaredoxin GRX2|TTR1 Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has two in-frame start codons resulting in a shorter isoform that is retained in the cytosol and a longer form translocated to the mitochondrial matrix; GRX2 has a paralog, GRX1, that arose from the whole genome duplication response to chemical, response to oxidative stress transferase activity, oxidoreductase activity nucleus, mitochondrion, cytoplasm BY4742 [4-8] G 4 YDR514C - - Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication biological_process molecular_function nucleus, mitochondrion, cytoplasm BY4742 [4-8] G 5 - nucleus, mitochondrion, cytoplasm nucleus, mitochondrion, cytoplasm nucleus, mitochondrion, cytoplasm - - - - [4-8] G 6 YDR516C EMI2 putative glucokinase Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress meiotic cell cycle, sporulation, transcription from RNA polymerase II promoter molecular_function cytoplasm BY4742 [4-8] G 7 YDR517W GRH1 - Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress Golgi vesicle transport molecular_function membrane, cytoplasmic vesicle, endomembrane system, cytoplasm, Golgi apparatus BY4742 [4-8] G 8 YDR518W EUG1 protein disulfide isomerase EUG1 Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER protein folding unfolded protein binding, isomerase activity, oxidoreductase activity endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [4-8] G 9 YDR519W FPR2 peptidylprolyl isomerase family protein FPR2|FKB2 Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress; mutation is functionally complemented by human FKBP2 biological_process isomerase activity nucleus, membrane, endomembrane system, vacuole, endoplasmic reticulum, cytoplasm BY4742 [4-8] G 10 YDR520C URC2 RRT4 Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism nucleobase-containing small molecule metabolic process DNA binding nucleus, cytoplasm BY4742 [4-8] G 11 YDR521W - - Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol biological_process molecular_function cellular_component BY4742 [4-8] G 12 YDR522C SPS2 - Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component meiotic cell cycle, sporulation, cell wall organization or biogenesis molecular_function cell wall, membrane, plasma membrane BY4742 [4-8] H 1 YDR523C SPS1 putative serine/threonine protein kinase SPS1 Putative protein serine/threonine kinase; localizes to the nucleus and cytoplasm; required for efficient spore packaging, prospore membrane development and closure and localization of enzymes involved in spore wall synthesis; interacts with and required for Ssp1p phosphorylation and turnover; member of the GCKIII subfamily of STE20 kinases; multiply phosphorylated on S/T residues; interacts with 14-3-3 proteins, Bmh1p and Bmh2p; expressed at the end of meiosis meiotic cell cycle, protein phosphorylation, sporulation, cell wall organization or biogenesis kinase activity, transferase activity nucleus, membrane, cytoplasm BY4742 [4-8] H 2 YDR524C AGE1 SAT1 ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif Golgi vesicle transport ion binding, lipid binding, enzyme regulator activity cytoplasmic vesicle, endomembrane system, cytoplasm, Golgi apparatus BY4742 [4-8] H 3 YDR525W API2 - Putative protein of unknown function; conserved among S. cerevisiae strains; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology biological_process molecular_function cellular_component BY4742 [4-8] H 4 - cellular_component cellular_component cellular_component - - - - [4-8] H 5 - - - - - - - - [4-8] H 6 YDR528W HLR1 - Protein involved in regulation of cell wall composition and integrity; also involved in cell wall response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; HLR1 has a paralog, LRE1, that arose from the whole genome duplication cell wall organization or biogenesis molecular_function cytoplasm BY4742 [4-8] H 7 YDR529C QCR7 ubiquinol--cytochrome-c reductase subunit 7|UCR7|CRO1|COR4 Subunit 7 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly mitochondrion organization, generation of precursor metabolites and energy, protein complex biogenesis, cellular respiration, nucleobase-containing small molecule metabolic process transmembrane transporter activity, oxidoreductase activity mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [4-8] H 8 YDR530C APA2 bifunctional AP-4-A phosphorylase/ADP sulfurylase Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication nucleobase-containing small molecule metabolic process hydrolase activity nucleus, cytoplasm BY4742 [4-8] H 9 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [4-8] H 10 YDR532C KRE28 - Subunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Spc105p; modified by sumoylation biological_process molecular_function nucleus, cytoskeleton, microtubule organizing center, chromosome BY4742 [4-8] H 11 YDR533C HSP31 glutathione-independent methylglyoxalase Methylglyoxalase that converts methylglyoxal to D-lactate; involved in oxidative stress resistance, diauxic shift, and stationary phase survival; has similarity to E. coli Hsp31 and C. albicans Glx3p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress response to chemical, response to oxidative stress, monocarboxylic acid metabolic process lyase activity cytoplasm BY4742 [4-8] H 12 YDR534C FIT1 - Mannoprotein that is incorporated into the cell wall; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell wall other molecular_function cell wall BY4742 [5-2] A 1 YEL001C IRC22 - Protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [5-2] A 2 - endomembrane system, endoplasmic reticulum, cytoplasm endomembrane system, endoplasmic reticulum, cytoplasm endomembrane system, endoplasmic reticulum, cytoplasm - - - - [5-2] A 3 YEL003W GIM4 tubulin-binding prefolding complex subunit GIM4|PFD2 Subunit of the heterohexameric cochaperone prefoldin complex; complex binds specifically to cytosolic chaperonin and transfers target proteins to it protein complex biogenesis cytoskeletal protein binding cytoplasm BY4742 [5-2] A 4 YEL004W YEA4 - Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter; required for cell wall chitin synthesis; localized to the ER carbohydrate metabolic process, transmembrane transport, ion transport, nucleobase-containing compound transport, cell wall organization or biogenesis transmembrane transporter activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [5-2] A 5 YEL005C VAB2 VAB31 Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Vps21p-GFP; has potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of; Vab2p-GFP-fusion localizes to cytoplasm in punctate pattern vesicle organization molecular_function cytoplasm BY4742 [5-2] A 6 YEL006W YEA6 NAD+ transporter|NDT2 Putative mitochondrial NAD+ transporter; member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog; YEA6 has a paralog, YIA6, that arose from the whole genome duplication transmembrane transport, nucleobase-containing compound transport other membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [5-2] A 7 YEL007W MIT1 TOS9 Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 biological_process molecular_function nucleus, cytoplasm BY4742 [5-2] A 8 YEL008W - - Putative protein of unknown function; conserved among S. cerevisiae strains; YEL008W is not an essential gene; predicted to be involved in metabolism biological_process molecular_function cellular_component BY4742 [5-2] A 9 YEL009C GCN4 amino acid starvation-responsive transcription factor GCN4|AAS101|ARG9|AAS3 bZIP transcriptional activator of amino acid biosynthetic genes; activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels response to chemical, protein complex biogenesis, DNA-templated transcription, initiation, response to starvation, transcription from RNA polymerase II promoter chromatin binding, nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding nucleus BY4742 [5-2] A 10 YEL010W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function cellular_component BY4742 [5-2] A 11 - cellular_component cellular_component cellular_component - - - - [5-2] A 12 - - - - - - - - [5-2] B 1 YEL013W VAC8 protein anchor VAC8|YEB3 Phosphorylated and palmitoylated vacuolar membrane protein; interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions vacuole organization, protein targeting, organelle assembly, organelle fusion, vesicle organization, membrane fusion, organelle inheritance other vacuole, membrane, cytoplasm BY4742 [5-2] B 2 YEL014C - - Putative protein of unknown function; conserved among S. cerevisiae strains biological_process molecular_function cellular_component BY4742 [5-2] B 3 YEL015W EDC3 LSM16|DCP3 Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; mediates decay of the RPS28B mRNA via binding to both Rps28Bp (or Rps28Ap) and the RPS28B mRNA; mediates decay of the YRA1 mRNA by a different, translation-independent mechanism; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress RNA catabolic process, organelle assembly RNA binding, mRNA binding nucleus, cytoplasm BY4742 [5-2] B 4 YEL016C NPP2 nucleotide diphosphatase/phosphodiesterase NPP2 Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; involved in spore wall assembly; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; NPP2 has a paralog, NPP1, that arose from the whole genome duplication; npp1 npp2 double mutant exhibits reduced dityrosine fluorescence relative to single mutants nucleobase-containing small molecule metabolic process nuclease activity, hydrolase activity endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [5-2] B 5 YEL017C-A PMP2 proteolipid ATPase Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication ion transport molecular_function membrane, vacuole, cytoplasm BY4742 [5-2] B 6 YEL017W GTT3 - Protein of unknown function may be involved in glutathione metabolism; function suggested by computational analysis of large-scale protein-protein interaction data; N- and C-terminal fusion proteins localize to the cell periphery biological_process molecular_function nucleus BY4742 [5-2] B 7 YEL018W EAF5 - Non-essential subunit of the NuA4 acetyltransferase complex; Esa1p-associated factor; relocalizes to the cytosol in response to hypoxia DNA repair, cellular response to DNA damage stimulus molecular_function nucleus, cytoplasm BY4742 [5-2] B 8 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [5-2] B 9 YEL020C PXP1 putative indolepyruvate decarboxylase family protein Peroxisomal matrix protein; well-conserved in fungi; contains tripartite homology domain of thiamine pyrophosphate (TPP) enzymes; targeted to peroxisomes by Pex5p; contains low sequence identity with Pdc1p; mRNA identified as translated by ribosome profiling data biological_process molecular_function peroxisome, cytoplasm BY4742 [5-2] B 10 - peroxisome, cytoplasm peroxisome, cytoplasm peroxisome, cytoplasm - - - - [5-2] B 11 - - - - - - - - [5-2] B 12 YEL023C - - Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene biological_process molecular_function cellular_component BY4742 [5-2] C 1 YEL024W RIP1 ubiquinol--cytochrome-c reductase catalytic subunit RIP1 Ubiquinol-cytochrome-c reductase; a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly generation of precursor metabolites and energy, cellular respiration, nucleobase-containing small molecule metabolic process molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [5-2] C 2 YEL025C - SRI1 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus, cytoplasm BY4742 [5-2] C 3 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [5-2] C 4 YEL027W VMA3 H(+)-transporting V0 sector ATPase subunit c|CUP5|GEF2|CLS7 Proteolipid subunit c of the V0 domain of vacuolar H(+)-ATPase; dicyclohexylcarbodiimide binding subunit; required for vacuolar acidification and important for copper and iron metal ion homeostasis vacuole organization, protein targeting, ion transport, cellular ion homeostasis, endocytosis transmembrane transporter activity vacuole, membrane, cytoplasm BY4742 [5-2] C 5 YEL028W - - Putative protein of unknown function; conserved among S. cerevisiae strains; YEL028C is not an essential gene biological_process molecular_function cellular_component BY4742 [5-2] C 6 YEL029C BUD16 putative pyridoxal kinase BUD16 Putative pyridoxal kinase; a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) mitotic cell cycle, cofactor metabolic process, cytokinesis kinase activity, transferase activity cellular_component BY4742 [5-2] C 7 YEL030W ECM10 Hsp70 family ATPase ECM10|SSC3 Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication protein targeting, protein folding, mitochondrion organization molecular_function mitochondrion, cytoplasm BY4742 [5-2] C 8 YEL031W SPF1 ion-transporting P-type ATPase SPF1|PER9|PIO1|COD1 P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 transmembrane transport, cellular ion homeostasis hydrolase activity, transmembrane transporter activity, ATPase activity endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm BY4742 [5-2] C 9 - endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm - - - - [5-2] C 10 YEL033W MTC7 - Protein of unknown function; predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant biological_process molecular_function cellular_component BY4742 [5-2] C 11 - cellular_component cellular_component cellular_component - - - - [5-2] C 12 - - - - - - - - [5-2] D 1 YEL036C ANP1 MNN8|GEM3 Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol protein glycosylation transferase activity, transferring glycosyl groups, transferase activity membrane, endomembrane system, cytoplasm, Golgi apparatus BY4742 [5-2] D 2 YEL037C RAD23 - Protein with ubiquitin-like N terminus; subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover; Rad4p-Rad23p heterodimer binds to promoters of DNA damage response genes to repress their transcription in the absence of DNA damage response to chemical, proteolysis involved in cellular protein catabolic process, transcription from RNA polymerase II promoter protein binding, bridging, hydrolase activity, DNA binding, ubiquitin-like protein binding nucleus, mitochondrion, cytoplasm BY4742 [5-2] D 3 YEL038W UTR4 putative acireductone synthase UTR4 Protein with sequence similarity to acireductone synthases; involved in methionine salvage; found in both the cytoplasm and nucleus cellular amino acid metabolic process phosphatase activity, hydrolase activity nucleus, cytoplasm BY4742 [5-2] D 4 YEL039C CYC7 cytochrome c isoform 2 Cytochrome c isoform 2, expressed under hypoxic conditions; also known as iso-2-cytochrome c; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication nucleobase-containing small molecule metabolic process, generation of precursor metabolites and energy, cellular respiration other mitochondrial envelope, mitochondrion, cytoplasm BY4742 [5-2] D 5 YEL040W UTR2 CRH2 Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck carbohydrate metabolic process, cell wall organization or biogenesis transferase activity, transferring glycosyl groups, transferase activity cell cortex, cytoskeleton, cell wall, cellular bud, site of polarized growth, cytoplasm BY4742 [5-2] D 6 YEL041W YEF1 NADH/NAD(+) kinase ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication cofactor metabolic process, nucleobase-containing small molecule metabolic process kinase activity, transferase activity cellular_component BY4742 [5-2] D 7 YEL042W GDA1 guanosine diphosphatase Guanosine diphosphatase located in the Golgi; involved in the transport of GDP-mannose into the Golgi lumen, converting GDP to GMP after mannose is transferred to substrates; null mutants are defective in sporulation and pre-meiotic S phase entry; orthologous to human ENTPD6, a meiosis-associated non-obstructive azoospermia (NOA) related gene identified in GWAS studies protein glycosylation hydrolase activity endomembrane system, cytoplasm, Golgi apparatus BY4742 [5-2] D 8 YEL043W - - Predicted cytoskeleton protein involved in intracellular signaling; based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold biological_process molecular_function endomembrane system, ribosome, endoplasmic reticulum, cytoplasm BY4742 [5-2] D 9 YEL044W IES6 - Component of the INO80 chromatin remodeling complex; critical for INO80 function; involved in regulation of chromosome segregation and maintenance of normal centromeric chromatin structure; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes chromatin organization, chromosome segregation molecular_function nucleus, chromosome BY4742 [5-2] D 10 YEL045C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress biological_process molecular_function membrane BY4742 [5-2] D 11 YEL046C GLY1 threonine aldolase GLY1 Threonine aldolase; catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis cellular amino acid metabolic process lyase activity cytoplasm BY4742 [5-2] D 12 YEL047C FRD1 fumarate reductase|FRDS1 Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication response to chemical oxidoreductase activity membrane, ribosome, mitochondrion, plasma membrane, cytoplasm BY4742 [5-2] E 1 YEL048C TCA17 - Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; promotes association of TRAPPII-specific subunits with the TRAPP core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder protein complex biogenesis, Golgi vesicle transport, endosomal transport molecular_function cytoplasmic vesicle, endomembrane system, Golgi apparatus, cytoplasm BY4742 [5-2] E 2 YEL049W PAU2 seripauperin PAU2 Member of the seripauperin multigene family; encoded mainly in subtelomeric region; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme biological_process molecular_function cellular_component BY4742 [5-2] E 3 YEL050C RML2 mitochondrial 54S ribosomal protein RML2 Mitochondrial ribosomal protein of the large subunit (L2); has similarity to E. coli L2 ribosomal protein; mutant allele (fat21) causes inability to utilize oleate, and induce oleic acid oxidation; may interfere with activity of the Adr1p transcription factor mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [5-2] E 4 YEL051W VMA8 H(+)-transporting V1 sector ATPase subunit D Subunit D of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits cellular ion homeostasis ATPase activity, hydrolase activity, transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [5-2] E 5 YEL052W AFG1 - Protein that may act as a chaperone for cytochrome c oxidase subunits; conserved protein; may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain response to chemical, protein targeting, transmembrane transport, proteolysis involved in cellular protein catabolic process, peroxisome organization, response to oxidative stress molecular_function mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [5-2] E 6 YEL053C MAK10 NAA35 Non-catalytic subunit of the NatC N-terminal acetyltransferase; required for replication of dsRNA virus; expression is glucose-repressible; human NatC ortholog, Naa35, requires co-expression of the human catalytic subunit, Naa30, to functionally complement the null allele protein acylation transferase activity cytoplasm BY4742 [5-2] E 7 YEL054C RPL12A uL11|ribosomal 60S subunit protein L12A|L11|YL23|L15A|L12A Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication cytoplasmic translation, organelle assembly, ribosomal large subunit biogenesis, ribosome assembly structural constituent of ribosome, structural molecule activity ribosome, cytoplasm BY4742 [5-2] E 8 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [5-2] E 9 YEL056W HAT2 - Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing peptidyl-amino acid modification, protein acylation, chromatin organization, histone modification transferase activity, histone binding nucleus, cytoplasm BY4742 [5-2] E 10 YEL057C SDD1 - Protein of unknown function; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P; may have a role in telomere maintenance; target of UME6 regulation biological_process molecular_function cellular_component BY4742 [5-2] E 11 - cellular_component cellular_component cellular_component - - - - [5-2] E 12 - - - - - - - - [5-2] F 1 YEL059W HHY1 ENV6 Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking biological_process molecular_function membrane BY4742 [5-2] F 2 YEL060C PRB1 proteinase B|CVT1 Vacuolar proteinase B (yscB) with H3 N-terminal endopeptidase activity; serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress; PRB1 has a paralog, YSP3, that arose from the whole genome duplication sporulation, response to starvation peptidase activity, hydrolase activity vacuole, cytoplasm BY4742 [5-2] F 3 YEL061C CIN8 kinesin motor protein CIN8|SDS15|KSL2 Kinesin motor protein; involved in mitotic spindle assembly and chromosome segregation mitotic cell cycle, organelle fission, cytoskeleton organization, organelle assembly, chromosome segregation hydrolase activity, ATPase activity nucleus, cytoskeleton, chromosome, mitochondrion, cytoplasm BY4742 [5-2] F 4 YEL062W NPR2 nitrogen permease regulating protein NPR2 Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of the SEA complex, a coatomer-related complex that associates dynamically with the vacuole; Npr2p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and resulting in inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; homolog of human tumor suppressor NPRL2 vacuole organization, response to chemical, regulation of organelle organization, organelle assembly, ion transport, response to starvation, amino acid transport, signaling molecular_function vacuole, membrane, cytoplasm BY4742 [5-2] F 5 YEL063C CAN1 arginine permease CAN1 Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication transmembrane transport, ion transport, amino acid transport transmembrane transporter activity endomembrane system, mitochondrion, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [5-2] F 6 YEL064C AVT2 - Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [5-2] F 7 YEL065W SIT1 siderophore transporter|ARN3 Ferrioxamine B transporter; member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p transmembrane transport, cellular ion homeostasis transmembrane transporter activity cytoplasmic vesicle, endomembrane system, vacuole, membrane, cytoplasm BY4742 [5-2] F 8 YEL066W HPA3 D-amino-acid N-acetyltransferase D-Amino acid N-acetyltransferase that detoxifies D-amino acids; catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; acetylates histones and polyamines, also autoacetylates response to chemical, cellular amino acid metabolic process transferase activity nucleus, cytoplasm BY4742 [5-2] F 9 YEL067C - - Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [5-2] F 10 YEL068C - - Protein of unknown function; expressed at both mRNA and protein levels; SWAT-GFP and seamless-GFP fusion proteins localize to the endoplasmic reticulum and mCherry fusion protein localizes to the vacuole biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [5-2] F 11 - endomembrane system, endoplasmic reticulum, cytoplasm endomembrane system, endoplasmic reticulum, cytoplasm endomembrane system, endoplasmic reticulum, cytoplasm - - - - [5-2] F 12 - - - - - - - - [5-2] G 1 YEL071W DLD3 D-lactate dehydrogenase 2-hydroxyglutarate transhydrogenase, and minor D-lactate dehydrogenase; converts D-2-hydroxyglutarate (D-2HG), an oncometabolite, to alpha-ketoglutarate in the presence of FAD, with concomitant reduction of pyruvate to D-lactate; minor lactate dehydrogenase activity; component of the retrograde regulon that consists of genes whose expression are stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source; located in the cytoplasm monocarboxylic acid metabolic process ion binding, oxidoreductase activity cytoplasm BY4742 [5-2] G 2 YEL072W RMD6 - Protein required for sporulation biological_process molecular_function cellular_component BY4742 [5-2] G 3 - cellular_component cellular_component cellular_component - - - - [5-2] G 4 - - - - - - - - [5-2] G 5 - - - - - - - - [5-2] G 6 - - - - - - - - [5-2] G 7 - - - - - - - - [5-2] G 8 - - - - - - - - [5-2] G 9 - - - - - - - - [5-2] G 10 YER001W MNN1 alpha-1,3-mannosyltransferase MNN1 Alpha-1,3-mannosyltransferase; integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family protein glycosylation transferase activity, transferring glycosyl groups, transferase activity endomembrane system, cytoplasm, Golgi apparatus BY4742 [5-2] G 11 YER002W NOP16 - Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleus, nucleolus BY4742 [5-2] G 12 - nucleus, nucleolus nucleus, nucleolus nucleus, nucleolus - - - - [5-2] H 1 YER004W FMP52 - Protein of unknown function; localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation biological_process molecular_function membrane, endomembrane system, mitochondrial envelope, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [5-2] H 2 YER005W YND1 apyrase|YEJ5|APY1 Apyrase with wide substrate specificity; helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity protein glycosylation hydrolase activity membrane, cytoplasmic vesicle, endomembrane system, cytoplasm, Golgi apparatus BY4742 [5-2] H 3 - membrane, cytoplasmic vesicle, endomembrane system, cytoplasm, Golgi apparatus membrane, cytoplasmic vesicle, endomembrane system, cytoplasm, Golgi apparatus membrane, cytoplasmic vesicle, endomembrane system, cytoplasm, Golgi apparatus - - - - [5-2] H 4 YER007C-A TMA20 RBF20 Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress other RNA binding ribosome, cytoplasm BY4742 [5-2] H 5 YER007W PAC2 - Microtubule effector required for tubulin heterodimer formation; binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl protein folding, protein complex biogenesis cytoskeletal protein binding cellular_component BY4742 [5-2] H 6 - cellular_component cellular_component cellular_component - - - - [5-2] H 7 - - - - - - - - [5-2] H 8 YER010C - bifunctional 4-hydroxy-4-methyl-2-oxoglutarate aldolase/oxaloacetate decarboxylase Bifunctional HMG aldolase/oxaloacetate decarboxylase; requires divalent metal ions for activity; competitively inhibited by oxalate; forms a ring-shaped homotrimer; similar to members of the prokaryotic RraA family of class II (divalent metal ion dependent) pyruvate aldolases from the meta cleavage pathways of protocatechuate and gallate monocarboxylic acid metabolic process lyase activity cellular_component BY4742 [5-2] H 9 YER011W TIR1 GPI-anchored mannoprotein|SRP1 Cell wall mannoprotein; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking; member of the Srp1p/Tip1p family of serine-alanine-rich proteins biological_process structural molecule activity cell wall, vacuole, cytoplasm BY4742 [5-2] H 10 - cell wall, vacuole, cytoplasm cell wall, vacuole, cytoplasm cell wall, vacuole, cytoplasm - - - - [5-2] H 11 - - - - - - - - [5-2] H 12 YER014W HEM14 oxygen-dependent protoporphyrinogen oxidase Protoporphyrinogen oxidase; a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides cofactor metabolic process oxidoreductase activity membrane, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [5-3] A 1 YER014C-A BUD25 - Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern mitotic cell cycle, cytokinesis molecular_function cellular_component BY4742 [5-3] A 2 - cellular_component cellular_component cellular_component - - - - [5-3] A 3 YER016W BIM1 microtubule-binding protein BIM1|EB1|YEB1 Microtubule plus end-tracking protein; together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally; homolog of human end binding protein 1 (EB1) mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, protein complex biogenesis, chromosome segregation, regulation of cell cycle cytoskeletal protein binding, structural molecule activity cytoskeleton, microtubule organizing center, cytoplasm BY4742 [5-3] A 4 YER017C AFG3 AAA family ATPase AFG3|YTA10 Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging; expression of human homolog AFG3L2 can complement yeast yta12 afg3 double mutant cytoplasmic translation, protein targeting, mitochondrion organization, transmembrane transport, protein maturation, protein complex biogenesis peptidase activity, hydrolase activity, ATPase activity vacuole, mitochondrion, membrane, mitochondrial envelope, cytoplasm BY4742 [5-3] A 5 - vacuole, mitochondrion, membrane, mitochondrial envelope, cytoplasm vacuole, mitochondrion, membrane, mitochondrial envelope, cytoplasm vacuole, mitochondrion, membrane, mitochondrial envelope, cytoplasm - - - - [5-3] A 6 YER019W ISC1 inositol phosphosphingolipid phospholipase Inositol phosphosphingolipid phospholipase C; mitochondrial membrane localized; hydrolyzes complex sphingolipids to produce ceramide; activates genes required for non-fermentable carbon source metabolism during diauxic shift; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance; ortholog of mammalian neutral sphingomyelinase type 2 lipid metabolic process, response to osmotic stress hydrolase activity endomembrane system, mitochondrion, membrane, mitochondrial envelope, endoplasmic reticulum, cytoplasm BY4742 [5-3] A 7 YER019C-A SBH2 Arf family guanine nucleotide exchange factor SBH2|SEB2 Ssh1p-Sss1p-Sbh2p complex component; involved in protein translocation into the endoplasmic reticulum; SBH2 has a paralog, SBH1, that arose from the whole genome duplication protein targeting guanyl-nucleotide exchange factor activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [5-3] A 8 YER020W GPA2 guanine nucleotide-binding protein subunit alpha|SSP101 Nucleotide binding alpha subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p, has signaling role in response to nutrients; required for the recruitment of Ras-GTP at the plasma membrane and in the nucleus meiotic cell cycle, response to chemical, sporulation, pseudohyphal growth, signaling GTPase activity, hydrolase activity mitochondrion, membrane, cytoplasm, plasma membrane BY4742 [5-3] A 9 - mitochondrion, membrane, cytoplasm, plasma membrane mitochondrion, membrane, cytoplasm, plasma membrane mitochondrion, membrane, cytoplasm, plasma membrane - - - - [5-3] A 10 - - - - - - - - [5-3] A 11 - - - - - - - - [5-3] A 12 YER024W YAT2 carnitine O-acetyltransferase YAT2 Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane other transferase activity cytoplasm BY4742 [5-3] B 1 - cytoplasm cytoplasm cytoplasm - - - - [5-3] B 2 - - - - - - - - [5-3] B 3 - - - - - - - - [5-3] B 4 YER028C MIG3 - Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genesenvironment response to chemical, cellular response to DNA damage stimulus, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, chromosome BY4742 [5-3] B 5 - nucleus, chromosome nucleus, chromosome nucleus, chromosome - - - - [5-3] B 6 YER030W CHZ1 - Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress chromatin organization histone binding nucleus BY4742 [5-3] B 7 - nucleus nucleus nucleus - - - - [5-3] B 8 YER032W FIR1 PIP1 Protein involved in 3' mRNA processing; interacts with Ref2p; APCC(Cdh1) substrate; potential Cdc28p substrate mRNA processing molecular_function cellular bud, site of polarized growth BY4742 [5-3] B 9 YER033C ZRG8 - Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency cell wall organization or biogenesis molecular_function cellular bud, site of polarized growth, mitochondrion, cytoplasm BY4742 [5-3] B 10 YER034W - - Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus, cytoplasm BY4742 [5-3] B 11 YER035W EDC2 - RNA-binding protein that directly activates mRNA decapping; binds mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein increases in abundance and relocalizes to nucleolus and to nuclear foci upon DNA replication stress; EDC2 has a paralog, EDC1, that arose from the whole genome duplication RNA catabolic process, regulation of translation RNA binding, mRNA binding nucleus, nucleolus, cytoplasm BY4742 [5-3] B 12 - nucleus, nucleolus, cytoplasm nucleus, nucleolus, cytoplasm nucleus, nucleolus, cytoplasm - - - - [5-3] C 1 - - - - - - - - [5-3] C 2 - - - - - - - - [5-3] C 3 YER038W-A FMP49 - Mitochondrial protein of unknown function; almost completely overlaps ORF HVG1/YER039C biological_process molecular_function mitochondrion, cytoplasm BY4742 [5-3] C 4 YER039C HVG1 putative GDP-mannose transporter|YEM9 Protein of unknown function; HVG1 has a paralog, VRG4, that arose from the whole genome duplication biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [5-3] C 5 YER040W GLN3 nitrogen-responsive transcriptional regulator GLN3 Transcriptional activator of genes regulated by nitrogen catabolite repression; localization and activity regulated by quality of nitrogen source and Ure2p response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [5-3] C 6 YER041W YEN1 crossover junction endodeoxyribonuclease Holliday junction resolvase; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1; similar to S. cerevisiae endonuclease Rth1p DNA repair, cellular response to DNA damage stimulus nuclease activity, hydrolase activity nucleus, cytoplasm BY4742 [5-3] C 7 YER042W MXR1 peptide-methionine-S-sulfoxide reductase|msrA Methionine-S-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan; reduced activity of human homolog implicated in Alzheimer disease response to chemical, response to oxidative stress oxidoreductase activity nucleus, cytoplasm BY4742 [5-3] C 8 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [5-3] C 9 YER044C ERG28 BUD18 Endoplasmic reticulum membrane protein; may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p lipid metabolic process protein binding, bridging membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [5-3] C 10 YER044C-A MEI4 - Meiosis-specific protein involved in forming DSBs; involved in double-strand break (DSBs) formation during meiotic recombination; required for chromosome synapsis and production of viable spores meiotic cell cycle, organelle fission molecular_function nucleus, chromosome BY4742 [5-3] C 11 YER045C ACA1 - ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [5-3] C 12 - nucleus nucleus nucleus - - - - [5-3] D 1 YER046W-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SAP1/YER047C biological_process molecular_function cellular_component BY4742 [5-3] D 2 YER047C SAP1 putative AAA family ATPase SAP1 Putative ATPase of the AAA family; interacts with the Sin1p transcriptional repressor in the two-hybrid system biological_process ATPase activity, hydrolase activity cytoplasm BY4742 [5-3] D 3 YER048C CAJ1 - Nuclear type II J heat shock protein of the E. coli dnaJ family; contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly protein folding molecular_function nucleus BY4742 [5-3] D 4 YER049W TPA1 oxidative DNA demethylase Fe(II)/2-oxoglutarate-dependent dioxygenase family member; catalyzes the repair of methyl-base lesions in both ss and dsDNA by oxidative demethylation; Poly(rA)-binding protein involved in mRNA poly(A) tail length and mRNA stability; role in translation termination efficiency; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to human prolyl 4-hydroxylase OGFOD1; binds Fe(II) and 2-oxoglutarate RNA catabolic process, DNA repair, cellular response to DNA damage stimulus RNA binding, ion binding, oxidoreductase activity nucleus BY4742 [5-3] D 5 YER050C RSM18 mitochondrial 37S ribosomal protein RSM18 Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S18 ribosomal protein mitochondrial translation, mitochondrion organization structural molecule activity, structural constituent of ribosome ribosome, mitochondrion, cytoplasm BY4742 [5-3] D 6 YER051W JHD1 [Histone H3]-lysine-36 demethylase|KDM2|JHDM1 JmjC domain family histone demethylase specific for H3-K36; similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe chromatin organization, transcription from RNA polymerase II promoter, histone modification, DNA-templated transcription, elongation histone binding, oxidoreductase activity cellular_component BY4742 [5-3] D 7 YER052C HOM3 aspartate kinase|THR3|SIL4|BOR1 Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis cellular amino acid metabolic process kinase activity, transferase activity cytoplasm BY4742 [5-3] D 8 YER053C PIC2 Cu/Pi carrier Mitochondrial copper and phosphate carrier; imports copper and inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature cellular ion homeostasis, transmembrane transport, ion transport transmembrane transporter activity membrane, mitochondrion, cytoplasm BY4742 [5-3] D 9 YER054C GIP2 - Putative regulatory subunit of protein phosphatase Glc7p; involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p; GIP2 has a paralog, PIG2, that arose from the whole genome duplication carbohydrate metabolic process, protein dephosphorylation, generation of precursor metabolites and energy enzyme regulator activity cytoplasm BY4742 [5-3] D 10 YER055C HIS1 ATP phosphoribosyltransferase ATP phosphoribosyltransferase; a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control cellular amino acid metabolic process transferase activity, transferring glycosyl groups, transferase activity cellular_component BY4742 [5-3] D 11 YER056C FCY2 purine-cytosine permease|BRA7 Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress transmembrane transport, nucleobase-containing compound transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [5-3] D 12 YER056C-A RPL34A eL34|ribosomal 60S subunit protein L34A|L34e|L34A Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome other BY4742 [5-3] E 1 YER057C HMF1 putative isoleucine biosynthesis protein HMF1|HIG1 Member of the p14.5 protein family; functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro; HMF1 has a paralog, MMF1, that arose from the whole genome duplication biological_process molecular_function nucleus, mitochondrial envelope, mitochondrion, cytoplasm BY4742 [5-3] E 2 YER058W PET117 - Protein required for assembly of cytochrome c oxidase mitochondrion organization, protein complex biogenesis molecular_function mitochondrion, cytoplasm BY4742 [5-3] E 3 YER059W PCL6 - Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding; PCL6 has a paralog, PCL7, that arose from the whole genome duplication carbohydrate metabolic process, generation of precursor metabolites and energy enzyme regulator activity other BY4742 [5-3] E 4 YER060W FCY21 purine-cytosine permease Putative purine-cytosine permease; very similar to Fcy2p but cannot substitute for its function transmembrane transport transmembrane transporter activity membrane, plasma membrane BY4742 [5-3] E 5 YER060W-A FCY22 purine-cytosine permease Putative purine-cytosine permease; very similar to Fcy2p but cannot substitute for its function transmembrane transport transmembrane transporter activity membrane, vacuole, plasma membrane, cytoplasm BY4742 [5-3] E 6 YER061C CEM1 fatty acid synthase CEM1 Mitochondrial beta-keto-acyl synthase; possible role in fatty acid synthesis; required for mitochondrial respiration; human homolog OXSM can complement yeast cem1 null mutant lipid metabolic process, monocarboxylic acid metabolic process transferase activity mitochondrion, cytoplasm BY4742 [5-3] E 7 YER062C GPP2 HOR2|glycerol-1-phosphatase HOR2 DL-glycerol-3-phosphate phosphatase involved in glycerol biosynthesis; also known as glycerol-1-phosphatase; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; GPP2 has a paralog, GPP1, that arose from the whole genome duplication carbohydrate metabolic process, response to osmotic stress phosphatase activity, hydrolase activity nucleus, cytoplasm BY4742 [5-3] E 8 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [5-3] E 9 - - - - - - - - [5-3] E 10 YER065C ICL1 isocitrate lyase 1 Isocitrate lyase; catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose carbohydrate metabolic process, monocarboxylic acid metabolic process lyase activity cytoplasm BY4742 [5-3] E 11 - cytoplasm cytoplasm cytoplasm - - - - [5-3] E 12 YER066C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YER067W biological_process molecular_function cellular_component BY4742 [5-3] F 1 YER067W RGI1 - Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion or in response to DNA replication stress; RGI1 has a paralog, RGI2, that arose from the whole genome duplication generation of precursor metabolites and energy molecular_function nucleus, cytoplasm BY4742 [5-3] F 2 YER067C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF RGI1/YER067W biological_process molecular_function cellular_component BY4742 [5-3] F 3 YER068W MOT2 CCR4-NOT core ubiquitin-protein ligase subunit MOT2|SIG1|NOT4 Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication proteolysis involved in cellular protein catabolic process, RNA catabolic process, protein modification by small protein conjugation or removal, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation transferase activity, DNA binding cytoplasm BY4742 [5-3] F 4 YER068C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF ARG5,6/YER069W biological_process molecular_function cellular_component BY4742 [5-3] F 5 YER069W ARG5,6 argC|argB|bifunctional acetylglutamate kinase/N-acetyl-gamma-glutamyl-phosphate reductase Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine cellular amino acid metabolic process kinase activity, transferase activity, oxidoreductase activity mitochondrion, cytoplasm BY4742 [5-3] F 6 YER070W RNR1 ribonucleotide-diphosphate reductase subunit RNR1|SDS12|RIR1|CRT7 Major isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits; relative distribution to the nucleus increases upon DNA replication stress; RNR1 has a paralog, RNR3, that arose from the whole genome duplication nucleobase-containing small molecule metabolic process oxidoreductase activity nucleus, cytoplasm BY4742 [5-3] F 7 YER071C TDA2 - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function site of polarized growth, cytoplasm BY4742 [5-3] F 8 YER072W VTC1 PHM4|NRF1 Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity; protein abundance increases in response to DNA replication stress vacuole organization, organelle fusion, membrane fusion RNA binding, mRNA binding endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [5-3] F 9 YER073W ALD5 aldehyde dehydrogenase (NAD(P)(+)) ALD5 Mitochondrial aldehyde dehydrogenase; involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed monocarboxylic acid metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4742 [5-3] F 10 YER074W RPS24A eS24|ribosomal 40S subunit protein S24A|S24e|S24A|RPS24EA Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication cytoplasmic translation, rRNA processing, ribosomal small subunit biogenesis structural constituent of ribosome, structural molecule activity ribosome, mitochondrion, cytoplasm BY4742 [5-3] F 11 YER075C PTP3 tyrosine protein phosphatase PTP3 Phosphotyrosine-specific protein phosphatase; involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm response to chemical, protein phosphorylation, sporulation, protein dephosphorylation, regulation of protein modification process, conjugation, signaling, response to osmotic stress phosphatase activity, hydrolase activity nucleus, cytoplasm BY4742 [5-3] F 12 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [5-3] G 1 - - - - - - - - [5-3] G 2 - - - - - - - - [5-3] G 3 YER079W - - Putative protein of unknown function biological_process molecular_function nucleus, cytoplasm BY4742 [5-3] G 4 YER080W AIM9 FMP29 Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion, cytoplasm BY4742 [5-3] G 5 YER081W SER3 phosphoglycerate dehydrogenase SER3 3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase; 3PG dehydrogenase that catalyzes the first step in serine and glycine biosynthesis; also functions as an alpha-ketoglutarate reductase, converting alpha-ketoglutarate to D-2-hydroxyglutarate (D-2HG); localizes to the cytoplasm; SER3 has a paralog, SER33, that arose from the whole genome duplication cellular amino acid metabolic process oxidoreductase activity cytoplasm BY4742 [5-3] G 6 - cytoplasm cytoplasm cytoplasm - - - - [5-3] G 7 YER083C GET2 GET complex subunit GET2|RMD7|HUR2 Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Golgi vesicle transport other membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [5-3] G 8 YER084W - - Protein of unknown function; expressed at both mRNA and protein levels biological_process molecular_function cellular_component BY4742 [5-3] G 9 YER085C - - Putative protein of unknown function biological_process molecular_function cellular_component BY4742 [5-3] G 10 YER086W ILV1 threonine ammonia-lyase ILV1|ISO1 Threonine deaminase, catalyzes first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation cellular amino acid metabolic process lyase activity mitochondrion, cytoplasm BY4742 [5-3] G 11 YER087W AIM10 putative proline--tRNA ligase AIM10 Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss biological_process ligase activity mitochondrion, cytoplasm BY4742 [5-3] G 12 YER087C-A - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF AIM10/YER087W biological_process molecular_function cellular_component BY4742 [5-3] H 1 - cellular_component cellular_component cellular_component - - - - [5-3] H 2 - - - - - - - - [5-3] H 3 - - - - - - - - [5-3] H 4 - - - - - - - - [5-3] H 5 - - - - - - - - [5-3] H 6 - - - - - - - - [5-3] H 7 - - - - - - - - [5-3] H 8 - - - - - - - - [5-3] H 9 - - - - - - - - [5-3] H 10 - - - - - - - - [5-3] H 11 - - - - - - - - [5-3] H 12 - - - - - - - - [6-1] A 1 - - - - - - - - [6-1] A 2 - - - - - - - - [6-1] A 3 - - - - - - - - [6-1] A 4 - - - - - - - - [6-1] A 5 - - - - - - - - [6-1] A 6 YFL006W - - - - - - BY4742 [6-1] A 7 - - - - - - - - [6-1] A 8 - - - - - - - - [6-1] A 9 - - - - - - - - [6-1] A 10 - - - - - - - - [6-1] A 11 YFL011W HXT10 hexose transporter HXT10 Putative hexose transporter; expressed at low levels and expression is repressed by glucose transmembrane transport, carbohydrate transport transmembrane transporter activity vacuole, mitochondrion, membrane, cytoplasm, plasma membrane BY4742 [6-1] A 12 - vacuole, mitochondrion, membrane, cytoplasm, plasma membrane vacuole, mitochondrion, membrane, cytoplasm, plasma membrane vacuole, mitochondrion, membrane, cytoplasm, plasma membrane - - - - [6-1] B 1 - - - - - - - - [6-1] B 2 - - - - - - - - [6-1] B 3 - - - - - - - - [6-1] B 4 - - - - - - - - [6-1] B 5 YFL015C - - Putative protein of unknown function; conserved across S. cerevisiae strains; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene biological_process molecular_function cellular_component BY4742 [6-1] B 6 - cellular_component cellular_component cellular_component - - - - [6-1] B 7 - - - - - - - - [6-1] B 8 YFL018C LPD1 dihydrolipoyl dehydrogenase|HPD1 Dihydrolipoamide dehydrogenase; the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes; LPD1 has a paralog, IRC15, that arose from the whole genome duplication monocarboxylic acid metabolic process, cellular amino acid metabolic process oxidoreductase activity mitochondrion, cytoplasm BY4742 [6-1] B 9 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [6-1] B 10 - - - - - - - - [6-1] B 11 YFL020C PAU5 seripauperin PAU5 Member of the seripauperin multigene family; encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme biological_process molecular_function membrane, vacuole, cytoplasm BY4742 [6-1] B 12 YFL021W GAT1 MEP80|NIL1 Transcriptional activator of nitrogen catabolite repression genes; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p; different translational starts produce two major and two minor isoforms that are differentially regulated and localized response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding, transcription factor activity, protein binding nucleus, cytoplasm BY4742 [6-1] C 1 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [6-1] C 2 YFL023W BUD27 URI1 Unconventional prefoldin protein involved in translation initiation; required for correct assembly of RNAP I, II, and III in an Rpb5p-dependent manner; shuttles between nucleus and cytoplasm; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP translational initiation, protein complex biogenesis molecular_function ribosome, cytoplasm BY4742 [6-1] C 3 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [6-1] C 4 YFL025C BST1 PER17 GPI inositol deacylase of the endoplasmic reticulum (ER); negatively regulates COPII vesicle formation; prevents production of vesicles with defective subunits; required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules; functional ortholog of human PGAP1, mutation of which is associated with intellectual disability and encephalopathy response to chemical, vesicle organization, proteolysis involved in cellular protein catabolic process, Golgi vesicle transport, lipid metabolic process hydrolase activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [6-1] C 5 YFL026W STE2 alpha-factor pheromone receptor STE2 Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells response to chemical, conjugation, signaling, protein complex biogenesis signal transducer activity membrane, plasma membrane BY4742 [6-1] C 6 YFL027C GYP8 - GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport other enzyme regulator activity other BY4742 [6-1] C 7 YFL028C CAF16 putative ATP-binding cassette family ATPase CAF16 Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator transcription from RNA polymerase II promoter ATPase activity, hydrolase activity cytoplasm BY4742 [6-1] C 8 - cytoplasm cytoplasm cytoplasm - - - - [6-1] C 9 YFL030W AGX1 alanine--glyoxylate transaminase Alanine:glyoxylate aminotransferase (AGT); catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; similar to mammalian and plant alanine:glyoxylate aminotransferases; human homolog AGXT complements yeast null mutant cellular amino acid metabolic process transferase activity mitochondrion, cytoplasm BY4742 [6-1] C 10 YFL031W HAC1 transcription factor HAC1|IRE15|ERN4 Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; two functional forms of Hac1p are produced; translation initiation is repressed under non-stress conditions; protein abundance increases in response to DNA replication stress meiotic cell cycle, response to chemical, transcription from RNA polymerase II promoter, signaling nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [6-1] C 11 YFL032W - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene biological_process molecular_function cellular_component BY4742 [6-1] C 12 - cellular_component cellular_component cellular_component - - - - [6-1] D 1 YFL034W MIL1 - Predicted lipase; binds variant medium clathrin chain Apm2p and contributes to its membrane recruitment; putative integral membrane protein that interacts with Rpp0p component of ribosomal stalk biological_process molecular_function membrane BY4742 [6-1] D 2 - membrane membrane membrane - - - - [6-1] D 3 - - - - - - - - [6-1] D 4 YFL035C-B - - - - - - BY4742 [6-1] D 5 YFL036W RPO41 DNA-directed RNA polymerase Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation; Rpo41p also synthesizes RNA primers for mitochondrial DNA replication DNA replication, mitochondrion organization transferase activity, nucleotidyltransferase activity mitochondrion, cytoplasm BY4742 [6-1] D 6 - mitochondrion, cytoplasm mitochondrion, cytoplasm mitochondrion, cytoplasm - - - - [6-1] D 7 - - - - - - - - [6-1] D 8 - - - - - - - - [6-1] D 9 YFL040W - - Putative transporter; member of the sugar porter family; YFL040W is not an essential gene; may have a role in intracellular sterol transport transmembrane transport transmembrane transporter activity membrane, vacuole, cytoplasm BY4742 [6-1] D 10 YFL041W FET5 ferroxidase FET5 Multicopper oxidase; integral membrane protein with similarity to Fet3p; may have a role in iron transport ion transport oxidoreductase activity membrane, endomembrane system, vacuole, endoplasmic reticulum, cytoplasm BY4742 [6-1] D 11 - membrane, endomembrane system, vacuole, endoplasmic reticulum, cytoplasm membrane, endomembrane system, vacuole, endoplasmic reticulum, cytoplasm membrane, endomembrane system, vacuole, endoplasmic reticulum, cytoplasm - - - - [6-1] D 12 YFL043C - - - - - - BY4742 [6-1] E 1 YFL044C OTU1 ubiquitin-specific protease OTU1|YOD1 Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress protein modification by small protein conjugation or removal peptidase activity, hydrolase activity nucleus, cytoplasm BY4742 [6-1] E 2 - nucleus, cytoplasm nucleus, cytoplasm nucleus, cytoplasm - - - - [6-1] E 3 YFL046W FMP32 - Putative assembly factor for cytochrome c oxidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; has similarity to human MCUR1/CCDC90A mitochondrion organization, protein complex biogenesis molecular_function mitochondrion, cytoplasm BY4742 [6-1] E 4 YFL047W RGD2 - GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress signaling enzyme regulator activity cellular bud, site of polarized growth, cytoplasm BY4742 [6-1] E 5 YFL048C EMP47 - Integral membrane component of ER-derived COPII-coated vesicles; functionS in ER to Golgi transport; EMP47 has a paralog, EMP46, that arose from the whole genome duplication Golgi vesicle transport other cytoplasmic vesicle, endomembrane system, vacuole, membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm BY4742 [6-1] E 6 YFL049W SWP82 - Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions transcription from RNA polymerase II promoter ATPase activity, hydrolase activity nucleus, cytoplasm BY4742 [6-1] E 7 YFL050C ALR2 putative Mg(2+) transporter ALR2 Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition transmembrane transport, ion transport transmembrane transporter activity membrane, plasma membrane BY4742 [6-1] E 8 YFL051C - - Putative protein of unknown function; SWAT-GFP fusion protein localizes to the cell periphery while mCherry fusion protein localizes to both the cell periphery and vacuole; YFL051C is not an essential gene biological_process molecular_function other BY4742 [6-1] E 9 YFL052W ZNF1 DNA-binding domain containing protein|ROP1 Zinc cluster transcription factor that regulates respiratory growth; binds to promoters of genes involved in respiration, gluconeogenesis, and the glyoxylate shunt; required for normal carbon source utilization and stress response response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus BY4742 [6-1] E 10 YFL053W DAK2 dihydroxyacetone kinase Dihydroxyacetone kinase; required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation carbohydrate metabolic process, response to chemical kinase activity, transferase activity cellular_component BY4742 [6-1] E 11 YFL054C AQY3 - Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol transmembrane transport transmembrane transporter activity membrane BY4742 [6-1] E 12 YFL055W AGP3 - Low-affinity amino acid permease; may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition transmembrane transport, ion transport, amino acid transport transmembrane transporter activity endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane BY4742 [6-1] F 1 YFL056C endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane endomembrane system, membrane, endoplasmic reticulum, cytoplasm, plasma membrane - - - BY4742 [6-1] F 2 - - - - - - - - [6-1] F 3 - - - - - - - - [6-1] F 4 YFR001W LOC1 - Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; relocalizes from nucleus to cytoplasm upon DNA replication stress rRNA processing, ribosomal large subunit biogenesis, ribosomal small subunit biogenesis RNA binding, mRNA binding nucleus, nucleolus, cytoplasm BY4742 [6-1] F 5 - nucleus, nucleolus, cytoplasm nucleus, nucleolus, cytoplasm nucleus, nucleolus, cytoplasm - - - - [6-1] F 6 - - - - - - - - [6-1] F 7 - - - - - - - - [6-1] F 8 - - - - - - - - [6-1] F 9 YFR006W - putative Xaa-Pro dipeptidase Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene biological_process molecular_function cytoplasm BY4742 [6-1] F 10 YFR007W YFH7 AIM12 Putative kinase with similarity to the PRK/URK/PANK kinase subfamily; the PRK/URK/PANK subfamily of P-loop kinases is also known as phosphoribulokinase/uridine kinase/bacterial pantothenate kinase other kinase activity, transferase activity, ATPase activity, hydrolase activity cellular_component BY4742 [6-1] F 11 YFR008W FAR7 - Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress response to chemical, conjugation, signaling molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [6-1] F 12 YFR009W GCN20 putative AAA family ATPase GCN20 Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA regulation of translation, translational elongation ATPase activity, hydrolase activity ribosome, cytoplasm BY4742 [6-1] G 1 YFR010W UBP6 ubiquitin-specific protease UBP6 Ubiquitin-specific protease; situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains en bloc, rather than from the distal tip of the chain; negatively regulates degradation of ubiquitinated proteins by the proteasome; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance; human homolog UBP14 complements yeast null mutant protein modification by small protein conjugation or removal, proteolysis involved in cellular protein catabolic process peptidase activity, hydrolase activity other BY4742 [6-1] G 2 - other other other - - - - [6-1] G 3 YFR012W DCV1 - Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication biological_process molecular_function membrane BY4742 [6-1] G 4 - membrane membrane membrane - - - - [6-1] G 5 YFR014C CMK1 calmodulin-dependent protein kinase CMK1 Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication protein phosphorylation, signaling kinase activity, transferase activity cytoplasm BY4742 [6-1] G 6 YFR015C GSY1 glycogen (starch) synthase GSY1 Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress carbohydrate metabolic process, generation of precursor metabolites and energy transferase activity, transferring glycosyl groups, transferase activity mitochondrion, cytoplasm BY4742 [6-1] G 7 YFR016C - - Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFR016C is not an essential gene biological_process molecular_function cellular bud, cytoplasm BY4742 [6-1] G 8 YFR017C IGD1 - Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication carbohydrate metabolic process, generation of precursor metabolites and energy enzyme regulator activity cytoplasm BY4742 [6-1] G 9 YFR018C - - Putative protein of unknown function; SWAT-GFP and seamless GFP fusion proteins localize to the endoplasmic reticulum and mCherry fusion protein localizes to the vacuole biological_process molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [6-1] G 10 - endomembrane system, endoplasmic reticulum, cytoplasm endomembrane system, endoplasmic reticulum, cytoplasm endomembrane system, endoplasmic reticulum, cytoplasm - - - - [6-1] G 11 YFR020W CSS2 - Protein of unknown function, secreted when constitutively expressed; SWAT-GFP fusion protein localizes to the endoplasmic reticulum (ER) and extracellular region, while mCherry fusion protein localizes to the ER and vacuole; mRNA identified as translated by ribosome profiling data; CSS2 is a non-essential gene biological_process molecular_function extracellular region, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [6-1] G 12 YFR021W ATG18 phosphoinositide binding protein ATG18|SVP1|AUT10|CVT18|NMR1 Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood protein targeting, protein maturation, endosomal transport lipid binding, ubiquitin-like protein binding, ion binding cytoplasmic vesicle, endomembrane system, vacuole, membrane, cytoplasm BY4742 [6-1] H 1 YFR022W ROG3 ART7 Alpha-arrestin involved in ubiquitin-dependent endocytosis; contributes to desensitization of agonist-occupied alpha-factor receptor Ste2p by Rsp5p-independent internalization; PPXY motif-mediated binding of the ubiquitin ligase Rsp5p is not required for adaptation; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; SWAT-GFP and mCherry fusion proteins localize to the cytosol; ROG3 has a paralog, ROD1, that arose from the whole genome duplication response to chemical, conjugation, regulation of transport, signaling, endocytosis enzyme binding cytoplasm BY4742 [6-1] H 2 YFR023W PES4 - Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication biological_process molecular_function cellular_component BY4742 [6-1] H 3 - cellular_component cellular_component cellular_component - - - - [6-1] H 4 YFR024C-A LSB3 YFR024C Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication other molecular_function cellular bud, site of polarized growth, mitochondrion, cytoplasm BY4742 [6-1] H 5 - cellular bud, site of polarized growth, mitochondrion, cytoplasm cellular bud, site of polarized growth, mitochondrion, cytoplasm cellular bud, site of polarized growth, mitochondrion, cytoplasm - - - - [6-1] H 6 YFR026C ULI1 - Protein of unknown function; involved in and induced by the endoplasmic reticulum unfolded protein response (UPR); SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum response to chemical, signaling molecular_function endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [6-1] H 7 - endomembrane system, endoplasmic reticulum, cytoplasm endomembrane system, endoplasmic reticulum, cytoplasm endomembrane system, endoplasmic reticulum, cytoplasm - - - - [6-1] H 8 - - - - - - - - [6-1] H 9 - - - - - - - - [6-1] H 10 - - - - - - - - [6-1] H 11 - - - - - - - - [6-1] H 12 YFR031C-A RPL2A uL2|ribosomal 60S subunit protein L2A|L2|rp8|YL6|L5A|L2A|RPL5B Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication cytoplasmic translation structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [7-1] A 1 - ribosome, cytoplasm ribosome, cytoplasm ribosome, cytoplasm - - - - [7-1] A 2 YGL002W ERP6 - Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication Golgi vesicle transport molecular_function membrane, endomembrane system, mitochondrion, endoplasmic reticulum, cytoplasm BY4742 [7-1] A 3 YGL003C CDH1 HCT1 Activator of anaphase-promoting complex/cyclosome (APC/C); antagonist of the spindle assembly checkpoint; directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including: Cdc20p, Ase1p, Cin8p, Fin1p and Clb5p; partially active in metaphase, and fully active in anaphase; cell-cycle regulated mitotic cell cycle, organelle fission, cytoskeleton organization, regulation of organelle organization, proteolysis involved in cellular protein catabolic process, chromosome segregation, regulation of protein modification process, protein modification by small protein conjugation or removal, regulation of cell cycle enzyme regulator activity nucleus, cytoplasm BY4742 [7-1] A 4 YGL004C RPN14 - Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p proteolysis involved in cellular protein catabolic process, protein complex biogenesis molecular_function nucleus, cytoplasm BY4742 [7-1] A 5 YGL005C COG7 Golgi transport complex subunit COG7|COD5 Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Golgi vesicle transport, protein targeting molecular_function endomembrane system, mitochondrion, cytoplasm, Golgi apparatus BY4742 [7-1] A 6 YGL006W PMC1 calcium-transporting ATPase PMC1 Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a transmembrane transport, ion transport, cellular ion homeostasis hydrolase activity, transmembrane transporter activity, ATPase activity vacuole, membrane, cytoplasm BY4742 [7-1] A 7 YGL007W BRP1 - Putative protein of unknown function; conserved among S. cerevisiae strains; located in the upstream region of PMA1; deletion leads to polyamine resistance due to downregulation of PMA1 biological_process molecular_function cellular_component BY4742 [7-1] A 8 - cellular_component cellular_component cellular_component - - - - [7-1] A 9 YGL009C LEU1 3-isopropylmalate dehydratase LEU1 Isopropylmalate isomerase; catalyzes the second step in the leucine biosynthesis pathway cellular amino acid metabolic process lyase activity cytoplasm BY4742 [7-1] A 10 YGL010W MPO1 - Protein involved in metabolism of phytosphingosine; not an essential gene lipid metabolic process molecular_function membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [7-1] A 11 - membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm membrane, endomembrane system, endoplasmic reticulum, cytoplasm - - - - [7-1] A 12 YGL012W ERG4 delta(24(24(1)))-sterol reductase C-24(28) sterol reductase; catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol lipid metabolic process oxidoreductase activity membrane, endomembrane system, endoplasmic reticulum, cytoplasm BY4742 [7-1] B 1 YGL013C PDR1 drug-responsive transcription factor PDR1|TPE3|TPE1|TIL1|SMR2|NRA2|CYH3|BOR2|ANT1|AMY1 Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; relocalizes to the cytosol in response to hypoxia; PDR1 has a paralog, PDR3, that arose from the whole genome duplication response to chemical, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, cytoplasm BY4742 [7-1] B 2 YGL014W PUF4 YGL023 Member of the PUF protein family; PUF family is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors RNA catabolic process, regulation of translation, chromatin organization RNA binding, mRNA binding cytoplasm BY4742 [7-1] B 3 YGL015C - - Putative protein of unknown function; null mutants accumulate cargo in the Golgi biological_process molecular_function membrane BY4742 [7-1] B 4 YGL016W KAP122 PDR6 Karyopherin beta; responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance protein targeting, nuclear transport protein transporter activity nucleus, membrane, endomembrane system, cytoplasm BY4742 [7-1] B 5 - nucleus, membrane, endomembrane system, cytoplasm nucleus, membrane, endomembrane system, cytoplasm nucleus, membrane, endomembrane system, cytoplasm - - - - [7-1] B 6 - - - - - - - - [7-1] B 7 YGL019W CKB1 casein kinase 2 regulatory subunit CKB1 Beta regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases transcription from RNA polymerase I promoter, peptidyl-amino acid modification, protein phosphorylation, transcription from RNA polymerase III promoter, cellular response to DNA damage stimulus enzyme regulator activity nucleus, nucleolus BY4742 [7-1] B 8 - nucleus, nucleolus nucleus, nucleolus nucleus, nucleolus - - - - [7-1] B 9 YGL021W ALK1 protein kinase ALK1 Protein kinase; along with its paralog, ALK2, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of cell polarity factors in mitosis, proper localization of formins and polarity factors, and survival in cells that activate spindle assembly checkpoint; phosphorylated in response to DNA damage; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins mitotic cell cycle, protein phosphorylation, organelle fission kinase activity, transferase activity cellular_component BY4742 [7-1] B 10 - cellular_component cellular_component cellular_component - - - - [7-1] B 11 - - - - - - - - [7-1] B 12 YGL024W - SST3 Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF PGD1/YGL025C biological_process molecular_function membrane BY4742 [7-1] C 1 YGL025C PGD1 MED3|HRS1 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor DNA-templated transcription, initiation, transcription from RNA polymerase II promoter DNA binding, transcription factor activity, protein binding, transcription factor binding nucleus BY4742 [7-1] C 2 YGL026C TRP5 tryptophan synthase TRP5 Tryptophan synthase; catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis cellular amino acid metabolic process lyase activity nucleus, cytoplasm BY4742 [7-1] C 3 YGL027C CWH41 DER7|GLS1 Processing alpha glucosidase I; ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress carbohydrate metabolic process, protein glycosylation, cell wall organization or biogenesis hydrolase activity, hydrolase activity, acting on glycosyl bonds endomembrane system, vacuole, membrane, endoplasmic reticulum, cytoplasm BY4742 [7-1] C 4 YGL028C SCW11 putative glucan endo-1,3-beta-D-glucosidase Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p biological_process hydrolase activity, hydrolase activity, acting on glycosyl bonds cell wall BY4742 [7-1] C 5 - cell wall cell wall cell wall - - - - [7-1] C 6 - - - - - - - - [7-1] C 7 YGL031C RPL24A eL24|ribosomal 60S subunit protein L24A|L24e|rp29|YL21|L30A|L24A|RPL30A Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication cytoplasmic translation RNA binding, structural molecule activity, structural constituent of ribosome ribosome, cytoplasm BY4742 [7-1] C 8 YGL032C AGA2 - Adhesion subunit of a-agglutinin of a-cells; C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds response to chemical, conjugation other cell wall, endomembrane system, vacuole, endoplasmic reticulum, cytoplasm BY4742 [7-1] C 9 YGL033W HOP2 - Meiosis-specific protein that localizes to chromosomes; prevents synapsis between nonhomologous chromosomes and ensures synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair; heterodimer of Hop2p-Mnd1p stimulates the Dmc1p-mediated strand invasion meiotic cell cycle, organelle fission, DNA recombination, chromosome segregation DNA binding nucleus, chromosome BY4742 [7-1] C 10 YGL034C - - Putative protein of unknown function; conserved among S. cerevisiae strains; YGL034C is not an essential gene biological_process molecular_function cellular_component BY4742 [7-1] C 11 YGL035C MIG1 transcription factor MIG1|TDS22|SSN1|CAT4 Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion; activated in stochastic pulses of nuclear localization, shuttling between cytosol and nucleus depending on external glucose levels and its phosphorylation state response to chemical, response to starvation, transcription from RNA polymerase II promoter nucleic acid binding transcription factor activity, DNA binding nucleus, endomembrane system, cytoplasm BY4742 [7-1] C 12 YGL036W - - Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YGL036W is not an essential gene biological_process molecular_function cytoplasm BY4742 [7-1] D 1 YGL037C PNC1 nicotinamidase Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; lacks a peroxisomal targeting signal but is imported into peroxisomes via binding to Gpd1p; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress cofactor metabolic process, chromatin organization, nucleobase-containing small molecule metabolic process, regulation of DNA metabolic process hydrolase activity peroxisome, nucleus, cytoplasm BY4742 [7-1] D 2 - peroxisome, nucleus, cytoplasm peroxisome, nucleus, cytoplasm peroxisome, nucleus, cytoplasm - - - - [7-1] D 3 YGL039W - carbonyl reductase (NADPH-dependent) Aldehyde reductase; reduces aliphatic aldehyde substrates using NADH as cofactor; shown to reduce carbonyl compounds to chiral alcohols response to chemical oxidoreductase activity cytoplasm BY4742 [7-1] D 4 - cytoplasm cytoplasm cytoplasm - - - - [7-1] D 5 YGL041C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data biological_process molecular_function membrane BY4742 [7-1] D 6 YGL042C - - Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 biological_process molecular_function cellular_component BY4742 [7-1] D 7 YGL043W DST1 SII|S-II|TFIIS|P37|PPR2 General transcription elongation factor TFIIS; enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress transcription from RNA polymerase I promoter, transcription from RNA polymerase III promoter, protein complex biogenesis, DNA-templated transcription, termination, DNA-templated transcription, initiation, mRNA processing, transcription from RNA polymerase II promoter, DNA-templated transcription, elongation enzyme binding, DNA binding, transcription factor activity, protein binding nucleus BY4742 [7-1] D 8 - nucleus nucleus nucleus - - - - [7-1] D 9 YGL045W RIM8 ART9|YGL046W|PAL3 Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family meiotic cell cycle, organelle fission, invasive growth in response to glucose limitation, protein maturation, endocytosis molecular_function membrane, cytoplasm, plasma membrane BY4742 [7-1] D 10 YGL046W membrane, cytoplasm, plasma membrane membrane, cytoplasm, plasma membrane membrane, cytoplasm, plasma membrane - - - BY4742 [7-1] D 11 - - - - - - - - [7-1] D 12 - - - - - - - - [7-1] E 1 YGL049C TIF4632 translation initiation factor eIF4G|eIF4G2 Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication translational initiation, organelle assembly RNA binding, translation factor activity, RNA binding, mRNA binding cytoplasm BY4742 [7-1] E 2 YGL050W TYW3 tRNA methyltransferase TYW3 tRNA methyltransferase required for synthesis of wybutosine; a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions tRNA processing, RNA modification transferase activity, methyltransferase activity cellular_component BY4742 [7-1] E 3 - cellular_component cellular_component cellular_component - - - - [7-1] E 4 YGL053W PRM8 pheromone-regulated DUP240 family protein PRM8 Pheromone-regulated protein; contains with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family; PRM8 has a paralog, PRM9, that arose from a segmental duplication biological_process molecular_function membrane, endomembrane system, endoplasmic reticulum, plasma membrane, cytoplasm BY4742 [7-1] E 5 YGL054C ERV14 cornichon family protein COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p and Nha1p antiporter to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication mitotic cell cycle, meiotic cell cycle, cell budding, sporulation, cytokinesis, Golgi vesicle transport other cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, cytoplasm BY4742 [7-1] E 6 - cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, cytoplasm cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, cytoplasm cytoplasmic vesicle, endomembrane system, membrane, endoplasmic reticulum, cytoplasm - - - - [7-1] E 7 YGL056C SDS23 - Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; SDS23 has a paralog, SDS24, that arose from the whole genome duplication other molecular_function nucleus, cytoplasm BY4742 [7-1] E 8 YGL057C GEP7 - Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion, cytoplasm BY4742 [7-1] E 9 YGL058W RAD6 E2 ubiquitin-conjugating protein RAD6|PSO8|UBC2 Ubiquitin-conjugating enzyme (E2); involved in postreplication repair as a heterodimer with Rad18p, regulation of K63 polyubiquitination in response to oxidative stress, DSBR and checkpoint control as a heterodimer with Bre1p, ubiquitin-mediated N-end rule protein degradation as a heterodimer with Ubr1p, ERAD with Ubr1p in the absence of canonical ER membrane ligases, and Rpn4p turnover as part of proteasome homeostasis, in complex with Ubr2p and Mub1p mitotic cell cycle, meiotic cell cycle, response to chemical, organelle fission, chromatin organization, regulation of transport, proteolysis involved in cellular protein catabolic process, DNA-templated transcription, termination, DNA recombination, transcription from RNA polymerase II promoter, DNA repair, telomere organization, cellular response to DNA damage stimulus, histone modification, protein modification by small protein conjugation or removal, regulation of cell cycle hydrolase activity, transferase activity, DNA binding, ATPase activity nucleus, chromosome, cytoplasm BY4742 [7-1] E 10 YGL059W PKP2 protein kinase PKP2 Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress peptidyl-amino acid modification, protein phosphorylation kinase activity, transferase activity mitochondrion, cytoplasm BY4742 [7-1] E 11 YGL060W YBP2 YBH1 Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication mitotic cell cycle, organelle fission, cytoskeleton organization molecular_function nucleus, chromosome, cytoplasm BY4742 [7-1] E 12 - nucleus, chromosome, cytoplasm nucleus, chromosome, cytoplasm nucleus, chromosome, cytoplasm - - - - [7-1] F 1 YGL062W PYC1 pyruvate carboxylase 1 Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication carbohydrate metabolic process ligase activity cytoplasm BY4742 [7-1] F 2 YGL063W PUS2 pseudouridine synthase PUS2 Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification; mutation also affects pseudouridylation of some nuclear-encoded mRNAs; PUS2 has a paralog, PUS1, that arose from the whole genome duplication tRNA processing, RNA modification isomerase activity mitochondrion, cytoplasm BY4742 [7-1] F 3 YGL064C MRH4 ATP-dependent RNA helicase Mitochondrial ATP-dependent RNA helicase of the DEAD-box family; required for assembly of the large subunit of mitochondrial ribosomes; binds to the large subunit rRNA, 21S_rRNA; localizes to the matrix face of the mitochondrial inner membrane and associates with the large subunit precursor and with mature ribosomes organelle assembly, ribosomal large subunit biogenesis, ribosome assembly RNA binding, hydrolase activity, rRNA binding, ATPase activity, helicase activity ribosome, mitochondrion, cytoplasm BY4742 [7-1] F 4 - ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm ribosome, mitochondrion, cytoplasm - - - - [7-1] F 5 YGL066W SGF73 deubiquitination module subunit SGF73|SCA7 Subunit of DUBm module o